1. The potential application of gold-apoferritin nanocages conjugated with 2-amino-2-deoxy-glucose for imaging of breast cancer cells
- Author
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N. Tülin Güray, Tuğba Nur Aslan, Elif Asik, and Mürvet Volkan
- Subjects
Cell ,Breast Neoplasms ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Inorganic Chemistry ,Nanocages ,medicine ,Animals ,Humans ,Glucosamine ,010405 organic chemistry ,Chemistry ,Cancer ,medicine.disease ,Imaging agent ,In vitro ,Molecular Imaging ,Nanostructures ,0104 chemical sciences ,Nanomedicine ,medicine.anatomical_structure ,Apoptosis ,Colloidal gold ,Apoferritins ,MCF-7 Cells ,Biophysics ,Adenocarcinoma ,Gold - Abstract
Development of biocompatible and multifunctional nanoprobes for tumor targeting, imaging, and therapy still remains a great challenge. Herein, gold nanoparticles (AuNPs) were synthesized in the cavity of horse spleen apoferritin protein (HoSAF) and protein surface was labeled with 2-amino-2-deoxy-glucose (2DG) as a cell surface glucose transport protein specific targeting probe to study the feasibility of its usage as a computer tomography (CT) contrast agent with tumor targeting capability through in vitro experiments. 2DG conjugated and gold-loaded apoferritin (Au-HoSAF-2DG) nanoparticles (NPs) showed selective targeting for human breast adenocarcinoma (MCF-7) cells when compared to normal breast (MCF-10A) cells. This AuNP-based imaging agent was found to be non-cytotoxic in a given concentration range with an apoptotic effect upon longer exposure times towards MCF-7 cells, while MCF-10A cells were affected less. This selective cell death would also be useful for further cancer treatments with the ability of X-ray attenuation in in vitro X-ray and computed tomography (CT) imaging.
- Published
- 2020