146 results on '"Y Y, Pei"'
Search Results
2. Baicalin decreases SGK1 expression in the hippocampus and reverses depressive-like behaviors induced by corticosterone
- Author
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B.-Y. Wang, Y.-C. Li, H.-B. Li, M. Bai, L.-L. Wang, J.-D. Shen, and Y.-Y. Pei
- Subjects
medicine.medical_specialty ,Motor Activity ,Protein Serine-Threonine Kinases ,Hippocampus ,Immediate early protein ,Immediate-Early Proteins ,Random Allocation ,chemistry.chemical_compound ,Receptors, Glucocorticoid ,Glucocorticoid receptor ,Dietary Sucrose ,Corticosterone ,11-beta-Hydroxysteroid Dehydrogenase Type 2 ,Internal medicine ,medicine ,Animals ,Hippocampus (mythology) ,RNA, Messenger ,Swimming ,Flavonoids ,Brain-derived neurotrophic factor ,Depressive Disorder ,Dose-Response Relationship, Drug ,Chemistry ,Brain-Derived Neurotrophic Factor ,General Neuroscience ,Taste Perception ,Feeding Behavior ,Antidepressive Agents ,Disease Models, Animal ,Endocrinology ,SGK1 ,Baicalin ,Behavioural despair test - Abstract
The present study was to investigate whether baicalin can prevent repeated exogenous corticosterone injection-induced depressive-like behaviors and explore its possible mechanisms. After a 21-day treatment with baicalin (10 and 20 mg/kg), sucrose preference in the sucrose preference test (SPT) and immobility time in forced swimming test (FST) were observed, serum corticosterone levels and brain-derived neurotrophic factor (BDNF) contents in the hippocampus were examined by enzyme-linked immunosorbent assay (ELISA). In addition, quantitative real-time polymerase chain reaction (qPCR) and western blot were used to detect the mRNA and protein expression in the hippocampus. The results showed that 21-day cortiscosterone injections caused depressive-like behaviors in mice, including the reduced sucrose preference and increased duration of immobility. Baicalin reversed these behavioral changes described above and restored serum corticosterone levels. Additionally, baicalin up-regulated the mRNA and protein expression of glucocorticoid receptor (GR) and BDNF, accompanied with the down-regulation of serum- and glucocorticoid-regulated kinase 1 (SGK1) in the hippocampus. Moreover, baicalin significantly increased the protein expression of 11β-hydroxysteroid dehydrogenase-2 (11β-HSD2) in the hippocampus. The present results confirmed the antidepressant-like effects of baicalin in a mice model of depression induced by corticosterone and suggested that its mechanism was possibly involved in reducing serum corticosterone and thereby increasing BDNF in the hippocampus.
- Published
- 2015
- Full Text
- View/download PDF
3. Differential expression of two C-type lectins in grass carp Ctenopharyngodon idella and their response to grass carp reovirus
- Author
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C S, Ju, L B, He, Y Y, Pei, Y, Jiang, R, Huang, Y M, Li, L J, Liao, S H, Jang, and Y P, Wang
- Subjects
Fish Proteins ,Carps ,DNA, Complementary ,Embryo, Nonmammalian ,Galectins ,Embryonic Development ,Gene Expression Regulation, Developmental ,Reoviridae ,Mannose-Binding Lectin ,Reoviridae Infections ,Fish Diseases ,Liver ,Animals ,RNA, Messenger ,Cloning, Molecular ,Intestinal Mucosa - Abstract
The cDNAs of two C-type lectins in grass carp Ctenopharyngodon idella, galactose-binding lectin (galbl) and mannose-binding lectin (mbl), were cloned and analysed in this study. Both of them exhibited the highest expression level in liver, whereas their expression pattern differed in early phase of embryonic development. Following exposure to grass carp reovirus (GCRV), the mRNA expression level of galbl and mbl was significantly up-regulated in liver and intestine.
- Published
- 2015
4. Characterizations of four toll-like receptor 4s in grass carp Ctenopharyngodon idellus and their response to grass carp reovirus infection and lipopolysaccharide stimulation
- Author
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Y Y, Pei, R, Huang, Y M, Li, L J, Liao, Z Y, Zhu, and Y P, Wang
- Subjects
Fish Proteins ,Lipopolysaccharides ,Toll-Like Receptor 4 ,Fish Diseases ,Carps ,Gene Expression Regulation ,Host-Pathogen Interactions ,Animals ,Reoviridae ,Reoviridae Infections - Abstract
In this study, the subcellular localization, tissue distribution and response to grass carp reovirus (GCRV) infection and lipopolysaccharide (LPS) stimulation of four grass carp Ctenopharyngodon idellus toll-like receptor 4 (tlr4) genes were investigated. All four genes were constitutively expressed in all tissues studied, but the subcellular localization and tissue exhibiting the highest expression differed for each protein. Following GCRV infection, all the four tlr4s were upregulated in all tissues examined, and stimulation of C. idellus kidney (CIK) cells with LPS resulted in downregulation of all four tlr4s. These results provide a foundation for further investigation of tlr4 genes in bony fishes.
- Published
- 2014
5. Enhancement of dissolution of cyclosporine A using solid dispersions with polyoxyethylene (40) stearate
- Author
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C, Liu, S J, Zhu, Y, Zhou, Y P, Wei, and Y Y, Pei
- Subjects
Diglycerides ,Excipients ,Chemical Phenomena ,Solubility ,Chemistry, Physical ,Cyclosporine ,Microscopy, Electron, Scanning ,Thermodynamics ,Crystallography, X-Ray ,Algorithms ,Immunosuppressive Agents ,Polyethylene Glycols - Abstract
A solid dispersion containing cyclosporine A (CyA) and polyoxyethylene (40) stearate (PS) was prepared by the solvent-melt method and characterized by powder X-ray diffraction (PXRD), hot-stage microscopy (HSM), scanning electron microscopy (SEM) and dissolution studies. The crystalline peaks of CyA disappeared in the PXRD spectra of solid dispersions but were seen in those of physical mixtures, demonstrating the amorphous state of the drug in solid dispersions. The solubility of CyA in aqueous solutions of PS was increased linearly with increasing amount of PS in water. Dissolution of the drug from solid dispersions and physical mixtures was dramatically enhanced compared to the drug powder alone in water at 37 degrees C.
- Published
- 2006
6. PEGylated recombinant human tumor necrosis factor alpha: preparation and anti-tumor potency
- Author
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Y P, Li, Y Y, Pei, J, Ding, Z M, Shen, X Y, Zhang, Z H, Gu, and J J, Zhou
- Subjects
Male ,Mice ,Tumor Necrosis Factor-alpha ,Tumor Cells, Cultured ,Animals ,Succinimides ,Antineoplastic Agents ,Sarcoma 180 ,Protein Processing, Post-Translational ,Recombinant Proteins ,Polyethylene Glycols - Abstract
To assess the merits of polyethylene glycol-modified recombinant human tumor necrosis factor alpha (PEG-rHuTNF-alpha).The rHuTNF-alpha was modified with N-succinimidyl succinate monomethoxy polyethylene glycol (SS-PEG) of three different molecular weights. The PEG-rHuTNF-alpha was separated into fractions of various molecular weights by gel filtration chromatography. In vitro activities of various fractions were determined with L929 cell assay and in vivo anti-tumor potencies of main fractions were studied with respect to necrosis of S-180 solid tumor.The rHuTNF-alpha could be modified using SS-PEG under mild conditions. The main fraction of PEG5000-rHuTNF-alpha contained four PEG molecules, and PEG12000-rHuTNF-alpha and PEG20000-rHuTNF-alpha contained two PEG molecules, respectively. There was a higher activity when rHuTNF-alpha was coupled to less numbers of the same molecular weight PEG molecules. When PEG-rHuTNF-alpha was of the same molecular weight, rHuTNF-alpha modified with bigger molecular weight PEG molecules had a higher activity. PEG-rHuTNF-alpha was resistant to proteolysis, and over 70 % activity remained after 8 h, but the activity of rHuTNF-alpha was time-dependently diminished by incubation with bovine trypsin. PEG5000-rHuTNF-alpha (1500 IU per mouse) had a similar anti-tumor potency compared with rHuTNF-alpha (3000 IU per mouse). PEG12000-rHuT NF-alpha (1500 IU per mouse) had an increased anti-tumor potency compared with rHuTNF-alpha (3000 IU per mouse). In particular, PEG20000-rHuTNF-alpha at a dose of 1500 IU per mouse had a higher anti-tumor potency than rHuTNF-alpha at a dose of 6000 IU per mouse.PEG-modified rHuTNF-alpha could be more suitable for therapeutic use
- Published
- 2001
7. Preparation and dissolution property of ipriflavone solid dispersion
- Author
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Y P, Li, X Y, Zhang, J J, Zhou, and Y Y, Pei
- Subjects
Solubility ,Technology, Pharmaceutical ,Isoflavones - Abstract
To prepare and identify ipriflavone (IP) solid dispersion, and determine its dissolution property.The solvent method was used for preparation and differential scanning calorimetry (DSC), X-ray diffraction and infrared spectrophotometry for identification of IP solid dispersion. The dissolution of the dispersion was determined with paddle method.The dissolution of IP solid dispersion consisting of IP and povidone-k30 (PVP) (1:8) in artificial gastric juice is 6.15 times as high as that of IP alone. The DSC curves, X-ray diffraction patterns and infrared spectrophotometries of IP have been changed obviously by the dispersion.The dissolution of IP is increased by solid dispersion method.
- Published
- 2001
8. Pharmacokinetics of intragastric ipriflavone solid dispersion in rats
- Author
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Y P, Li, J J, Zhou, X Y, Zhang, and Y Y, Pei
- Subjects
Male ,Rats, Sprague-Dawley ,Animals ,Biological Availability ,Bone Remodeling ,Isoflavones ,Rats - Abstract
To evaluate pharmacokinetic behavior of ipriflavone solid dispersion in rats.The plasma concentrations of ipriflavone in rats were determined by HPLC with UV detector.Plasma concentration-time curves after ig ipriflavone solid dispersion 250 mg.kg-1 in rats were fitted with one-compartment model. Pharmacokinetic parameters were as follows: Ke = 0.21 h-1, T1/2Ke = 5.19 h, Ka = 1.71 h-1, T1/2Ka = 0.41 h, Tmax = 0.67 h, Cmax = 429 micrograms.L-1, AUC = 3916 micrograms.h.L-1; The relative bioavailability of ipriflavone solid dispersion was 323%.Ipriflavone in solid dispersion was absorbed more effectively than that in physical mixture in rats.
- Published
- 2001
9. An improved HPLC assay for ciprofloxacin in biological samples
- Author
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Y Y, Pei, X, Meng, and C H, Nightingale
- Subjects
Ciprofloxacin ,Animals ,Humans ,Rabbits ,Sensitivity and Specificity ,Chromatography, High Pressure Liquid - Abstract
A simple and sensitive high performance liquid chromatographic method was developed for determination of ciprofloxacin (Cip) in 0.1 ml of rabbit and human serum and CSF. Separation of Cip and pipemidic acid (internal standard) was achieved on Nucleosil C18 (4.6 mm x 250 mm) using fluorescence detection with lambda exc 274 nm and lambda emi 418 nm. The mobile phase was composed of acetonitrile and phosphate buffer 10 mmol.L-1 (pH 2.7) containing tetrabutylammonium hydrogen sulfate 5 mmol.L-1 (18:82, vol:vol) and the flow rate was set at 1.0 ml.min-1. The retention times were 5.9 min for Cip and 4.0 min for pipemidic acid. The inter-day coefficient of variation was6.97% (n = 5) and the intra-day coefficient of variation was3.33% (n = 5). The limits of detection were 3 ng.ml-1 serum and 5 ng.ml-1 CSF, (ror = 0.9996). Application of this method was demonstrated with simultaneous measurements of concentration-time profiles of Cip in rabbit serum and CSF during iv infusions at constant rates of 0.33, 1.0, and 2.5 mg.kg-1.h-1.
- Published
- 1994
10. Clinical study on the pharmacokinetics of lithium carbonate
- Author
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J S, Ku, Z Y, Wu, Y S, Fang, and Y Y, Pei
- Subjects
Adult ,Male ,Lithium Carbonate ,Humans ,Female ,Lithium ,Middle Aged ,Models, Biological - Abstract
To investigate clinical pharmacokinetics of lithium carbonate, 28 hospitalized mental patients and 8 healthy volunteers were studied. The serum concentration/time profile in the single dose peroral experiments could be fitted to a pharmacokinetic model using an open two compartmental system. Three methods, 72-h and 24-h residual method and Ritschel's repeated one-point method were used and the results compared with one another. According to the results of this paper, we suggest the use of Ritschel's repeated one-point method for clinical dosage regimen determination.
- Published
- 1987
11. [Relation of in vitro release of the isosorbide dinitrate to blood pressure changes after administration to anesthetized cats]
- Author
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Y M, Wang, Y Z, Han, Y D, Shao, N Z, Xi, Y Y, Pei, and J G, Chen
- Subjects
Male ,Delayed-Action Preparations ,Cats ,Animals ,Anesthesia ,Blood Pressure ,Female ,Isosorbide Dinitrate - Published
- 1981
12. Sentinel-1 TOPS interferometry for along-track displacement measurement.
- Author
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H J Jiang, Y Y Pei, and J Li
- Published
- 2017
- Full Text
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13. [A Mendelian randomization study on the relationship between insomnia and osteoporosis].
- Author
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Liu HZ, Fu XM, Li XJ, Wang YH, Hu XD, Xu HJ, Wang AN, Lyu ZH, Dong S, and Pei Y
- Subjects
- Humans, Risk Factors, Osteoporosis genetics, Osteoporosis epidemiology, Mendelian Randomization Analysis, Sleep Initiation and Maintenance Disorders genetics, Sleep Initiation and Maintenance Disorders epidemiology, Polymorphism, Single Nucleotide, Genome-Wide Association Study
- Abstract
Objective: To explore the relationship between insomnia and osteoporosis. Methods: Mendelian randomization (MR) analysis were used in this study. The single nucleotide polymorphisms (SNPs) related to insomnia from genome-wide association analysis research data were selected as the instrumental variables by using inverse variance weighted (IVW), MR-Egger regression, weighted median method, maximum likelihood, penalized weighted median estimator, and Mendelian randomization robust adjusted profile score (MR-RAPS) to determine the causal relationship between insomnia and osteoporosis. Odds ratio ( OR ) and 95% confidence interval ( CI ) values were used to evaluate the association between insomnia and osteoporosis. Cochran's Q -test was used to detect heterogeneity of SNPs, MR-Egger regression was used to test for level pleiotropy, and the leave-one-out method was used to test sensitivity, MR pleiotropy residual sum and outlier (MR-PRESSO) method and radial MR were used to detect erroneous outliers. Results: The screening criteria were set based on the three major assumptions of MR; finally, 31 SNPs were included in the MR analysis. The results of MR causal effect analysis using the IVW method showed that insomnia increased the risk of osteoporosis by about 0.7% ( OR =1.007, 95% CI 1.001-1.014, P =0.044); heterogeneity testing showed heterogeneity between SNPs ( Q =57.91, P <0.001); and the MR- Egger intercept test did not indicate horizontal pleiotropy in this study (intercept value=3.807×10
-5 , P =0.888). Leave-one-out method showed that no single SNP had a significant impact on the overall results. No abnormal SNP was detected according to the MR-PRESSO results ( P =0.059), and radial MR did not detect any outliers. Conclusion: Mendelian randomization analysis showed that insomnia can increase the risk of osteoporosis.- Published
- 2024
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14. [Clinical analysis of adverse reactions in patients with multidrug-resistant and rifampicin-resistant pulmonary tuberculosis treated with delamanid-containing regimen].
- Author
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Gao MQ, Gao JT, Ma XG, Shu W, Du J, Liang RX, Wu GH, Pei Y, Yan XF, Cai QS, Lyu KY, Cai C, Wu YQ, Li XJ, Liu QQ, Jin L, Wu QH, Xiong Y, Li MW, Zhou YQ, Kuang HB, Wang XF, Ren F, Chen XH, Geng SJ, Zhou Y, Sha W, Yang GL, Wang H, Zhan Y, Liu YH, and Li L
- Subjects
- Humans, Male, Female, Adult, Prospective Studies, Middle Aged, Aged, China, Young Adult, Drug-Related Side Effects and Adverse Reactions etiology, Tuberculosis, Multidrug-Resistant drug therapy, Rifampin adverse effects, Oxazoles adverse effects, Oxazoles therapeutic use, Oxazoles administration & dosage, Antitubercular Agents adverse effects, Tuberculosis, Pulmonary drug therapy, Nitroimidazoles adverse effects, Nitroimidazoles therapeutic use, Nitroimidazoles administration & dosage
- Abstract
Objective: To explore the characteristics of adverse drug reactions during the 24-week therapy with delamanid-containing regimen for patients with multidrug-resistant and rifampicin-resistant pulmonary tuberculosis (MDR/RR-PTB). Methods: The prospective multicenter study was conducted from June 2020 to June 2023. A total of 608 eligible patients with MDR/RR-PTB were enrolled in 26 tuberculosis medical institutions in China including 364 males and 79 females, aged 39.6(19.0-68.0) years. Patients were treated with chemotherapy regimens containing delamanid. Patients were closely supervised during treatment of medication, and all adverse reactions occurring during treatment were monitored and recorded. The clinical characteristics of adverse reactions were evaluated by descriptive analysis. Chi-square test and multivariate logistic regression were used to analyze the related factors of QTcF interval prolongation (QT corrected with Fridericia's formula). Results: Of the 608 patients enrolled in this study, 325 patients (53.5%) reported 710 adverse events within 24 weeks of treatment. The top 6 most common complications were hematological abnormalities (143 patients, 23.5%), QT prolongation (114 patients, 18.8%), liver toxicity (85 patients, 14.0%), gastrointestinal reaction (41 patients, 6.7%), peripheral neuropathy (25 patients, 4.1%) and mental disorders (21 patients, 3.5%). The prolongation of QT interval mostly occurred in the 12th week after the first dose of medication. Serious adverse reactions occurred in 21 patients (3.5%). There were 7 patients (1.2%) with mental disorders, including 2 patients (0.3%) with severe mental disorders. Conclusions: The safety of dalamanid-based regimen in the staged treatment of MDR/RR-PTB patients was generally good, and the incidence of adverse reactions was similar to that reported in foreign studies. This study found that the incidence of QT interval prolongation in Chinese patients was higher than that reported overseas, suggesting that the monitoring of electrocardiogram should be strengthened when using drugs containing delamanid that may cause QT interval prolongation.
- Published
- 2024
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15. [Analysis of clinical effect of arthroscopic release at different time in treating knee adhesion after arthroscopic surgery].
- Author
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Zhao F, Zeng HY, Jia ST, Pei Y, Cheng X, Zhang X, Huang HJ, and Wang JQ
- Subjects
- Humans, Female, Male, Adult, Retrospective Studies, Tissue Adhesions, Treatment Outcome, Postoperative Complications, Time Factors, Arthroscopy, Range of Motion, Articular, Knee Joint surgery
- Abstract
Objective: To explore the efficacy of arthroscopic release in treating postoperative knee adhesion and investigate the influence of release timing on the treatment outcomes. Methods: A total of 50 patients who accepted arthroscopic release in Peking University Third Hospital from February 2017 to December 2021 were included in the retrospective cohort. The study cohort comprised 28 men and 22 women, with a mean age of (30.8±11.9) years. All the primary surgeries were manipulated under arthroscopes. A comparison was made between pre-and postoperative range of motion (ROM), visual analog scale (VAS), International Knee Documentation Committee (IKDC) scores, and Tegner activity scale scores for the patients. According to the interval between the appearance of adhesion and arthroscopic release, the patients were divided into four groups:<3 months group ( n =12), 3-6 months group ( n =16),>6-12 months group ( n =14), and>12 months group ( n =8). Inter-group comparisons on postoperative ROM, IKDC scores, and Tegner activity scale scores and improvement values of each outcome were conducted. Results: All the patients were followed up for (36.4±19.7) months. Patients gained significant improvement in flexion, extension, IKDC scores, and Tegner scores (125.0°±20.0° vs 75.7°±27.5°, 2.3°±4.8° vs 7.4°±7.3°, 69.8±17.7 vs 51.4±12.8, 4.1±2.1 vs 2.2±1.1) (all P <0.05), while the VAS scores did not show significant improvement. There were no significant differences among different groups in postoperative extension, IKDC scores or Tegner scores, nor in their improvements. However, patients in the ≤6 months group could gain better postoperative flexion and improvement in flexion than those in the >6 months group (129.9°±20.0° vs 118.8°±17.4°, 58.6°±32.8° vs 37.3°±23.1°) ( P <0.05). Conclusions: Arthroscopic release presents a great effect in treating knee adhesion after arthroscopic operation. Once the symptoms of adhesion appear and physical rehabilitation fails to improve the ROM, one should accept early surgical intervention (less than 6 months) for a better outcome.
- Published
- 2024
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16. Beneficial effects of pioglitazone and α-lipoic acid in patients with polycystic ovaries syndrome.
- Author
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Pei Y, Liu YY, Sun M, Zheng J, Zhou TT, Wang B, Hu H, and Wang ZL
- Subjects
- Female, Humans, Animals, Mice, Pioglitazone therapeutic use, Body Weight, Insulin, Thioctic Acid therapeutic use, Polycystic Ovary Syndrome drug therapy
- Abstract
Objective: Changes in hormone levels, improper lipid metabolism, and oxidative stress all significantly contribute to the pathogenic process of polycystic ovarian syndrome (PCOS). According to earlier research, pioglitazone and alpha-lipoic acid are crucial in the emergence of PCOS. The beneficial effects of pioglitazone and alpha-lipoic acid on PCOS were examined in the current study., Patients and Methods: The 120 patients with PCOS received three months of treatment in pioglitazone groups (n=40 case, 30 mg/time, 1 time/day), α-lipoic acid (n=40 case, 0.6 g/time, 1 time/day), and combination therapy (n=40 case, pioglitazone 30 mg/time, 1 time/day and α-lipoic acid, 0.6 g/time, 1 time/day). Before and after therapy, the following factors were evaluated: the hormonal profile, fasting serum insulin, body weight, body mass index (BMI), menstruation status, oxidative stress, and indications of lipid metabolism., Results: The combination of pioglitazone and α-lipoic acid has a significantly improving effect on BMI, body weight, oxidative stress levels, lipid metabolism, and menstrual status. A significant increase in body weight, BMI, and follicle-stimulating hormone (FSH) levels were found in mice after being treated with α-lipoic acid alone. However, the use pioglitazone alone improves body weight, BMI, the calculation of insulin resistance index (HOMA-IR), Area under the curve (AUC)-insulin, fasting glucose/insulin (G/I) ratio, total testosterone, and malondialdehyde (MDA) levels in post-treatment than pre-treatment., Conclusions: These findings suggest that pioglitazone alone has a better effect than alpha-lipoic acid in improving oxidative stress levels, BMI, and menstrual cyclicity. Additionally, treatment with pioglitazone and alpha-lipoic acid did demonstrate a greater effect than monotherapy with each medication alone.
- Published
- 2023
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17. Randomized phase II adjuvant trial to compare two treatment durations of icotinib (2 years versus 1 year) for stage II-IIIA EGFR-positive lung adenocarcinoma patients (ICOMPARE study).
- Author
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Lv C, Wang R, Li S, Yan S, Wang Y, Chen J, Wang L, Liu Y, Guo Z, Wang J, Pei Y, Yu L, Wu N, Lu F, Gao F, Chen J, Liu Y, Wang X, Li S, Han B, Zhang L, Ma Y, Ding L, Wang Y, Yuan X, and Yang Y
- Subjects
- Humans, Duration of Therapy, ErbB Receptors genetics, Carcinoma, Non-Small-Cell Lung drug therapy, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung pathology, Lung Neoplasms drug therapy, Lung Neoplasms genetics, Lung Neoplasms pathology, Adenocarcinoma of Lung drug therapy, Adenocarcinoma of Lung genetics
- Abstract
Background: Despite the prolonged median disease-free survival (DFS) by adjuvant targeted therapy in non-small-cell lung cancer patients with epidermal growth factor receptor (EGFR) mutations, the relationship between the treatment duration and the survival benefits in patients remains unknown., Patients and Methods: In this multicenter, randomized, open-label, phase II trial, eligible patients aged 18-75 years with EGFR-mutant, stage II-IIIA lung adenocarcinoma and who had not received adjuvant chemotherapy after complete tumor resection were enrolled from eight centers in China. Patients were randomly assigned (1 : 1) to receive either 1-year or 2-year icotinib (125 mg thrice daily). The primary endpoint was DFS assessed by investigator. The secondary endpoints were overall survival (OS) and safety. This study was registered at ClinicalTrials.gov (NCT01929200)., Results: Between September 2013 and October 2018, 109 patients were enrolled (1-year group, n = 55; 2-year group, n = 54). Median DFS was 48.9 months [95% confidence interval (CI) 33.1-70.1 months] in the 2-year group and 32.9 months (95% CI 26.6-44.8 months) in the 1-year group [hazard ratio (HR) 0.51; 95% CI 0.28-0.94; P = 0.0290]. Median OS for patients was 75.8 months [95% CI 64.4 months-not evaluable (NE)] in the 2-year group and NE (95% CI 66.3 months-NE) in the 1-year group (HR 0.34; 95% CI 0.13-0.95; P = 0.0317). Treatment-related adverse events (TRAEs) were observed in 41 of 55 (75%) patients in the 1-year group and in 36 of 54 (67%) patients in the 2-year group. Grade 3-4 TRAEs occurred in 4 of 55 (7%) patients in the 1-year group and in 3 of 54 (6%) patients in the 2-year group. No treatment-related deaths or interstitial lung disease was reported., Conclusions: Two-year adjuvant icotinib was shown to significantly improve DFS and provide an OS benefit in EGFR-mutant, stage II-IIIA lung adenocarcinoma patients compared with 1-year treatment in this exploratory phase II study., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2023
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18. [Diagnosis and treatment of aldosterone-producing adenoma presenting as bilateral adrenal lesions: a case report].
- Author
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Li H, Pei Y, Yang GQ, Li YJ, Ni Q, Chen YL, Ba JM, Lyu ZH, and Mu YM
- Subjects
- Humans, Aldosterone, Hyperaldosteronism diagnosis, Adrenal Gland Neoplasms diagnosis, Adrenal Gland Neoplasms complications, Adenoma diagnosis
- Published
- 2023
- Full Text
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19. The secret of H 2 S to keep plants young and fresh and its products.
- Author
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Liu D and Pei Y
- Subjects
- Plants, Reactive Oxygen Species, Hydrogen Sulfide
- Abstract
Recently, accumulating evidence has shown that hydrogen sulphide (H
2 S), a newly determined gasotransmitter, plays important roles in senescence, which is an essential biological process for plant fitness and an important agricultural trait that is critical for the yield and quality of farm produce. Here, in this review, we summarize the roles of H2 S in senescence, both before and after the harvesting of agricultural products, and the underlying mechanism is also discussed. During the plant growth process, the function of H2 S in the leaf senescence process has been studied extensively, and H2 S plays roles during the whole process, including the initiation, reorganization and terminal stages. While during the postharvest stage, H2 S can prevents farm products from deterioration resulting from over-ripening, pathogen attack and incorrect storage. The underlying H2 S-related mechanisms during different stages of the senescence process are summarized and compared. The most prominent interaction occurs between H2 S and reactive oxygen species, and the molecular mechanism is explored. Additionally, the conserved action mode of H2 S in different life processes and different species is also discussed. In the future, multi-omics analyses over time will be needed to investigate the detailed mechanisms of H2 S, and a safety attribute analysis of H2 S is also required before it can be used in agricultural production., (© 2021 German Society for Plant Sciences and The Royal Botanical Society of the Netherlands.)- Published
- 2022
- Full Text
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20. [Diagnosis and treatment of 21-hydroxylase deficiency with testicular adrenal rest tumors:a report of three cases and literature review].
- Author
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Zhang Q, Zang L, Zhang CY, Gu WJ, Li B, Jia XF, Chen K, Pei Y, Du J, Guo QH, Ba JM, Lyu ZH, Dou JT, and Mu YM
- Subjects
- Adult, Humans, Infant, Male, Retrospective Studies, Adrenal Hyperplasia, Congenital diagnosis, Adrenal Hyperplasia, Congenital drug therapy, Adrenal Rest Tumor diagnosis, Adrenal Rest Tumor drug therapy, Testicular Neoplasms diagnosis, Testicular Neoplasms therapy
- Abstract
Objective: To provide insight into the diagnosis for clinicians, the clinical characteristics, diagnosis and treatment history of 3 patients with 21-hydroxylase deficiency (21-OHD) and testicular adrenal rest tumors (TART) were analyzed. Methods: The clinical, laboratory and imaging data of 3 male patients with 21-OHD and TART, confirmed with CYP21 gene sequencing, from May 2010 to May 2021 in the First Medical Center of Chinese PLA General Hospital were analyzed retrospectively. The treatment strategy and clinical outcome were followed up. Results: All the 3 patients were first diagnosed with bilateral adrenal mass at the age of 27-42 years old. They were 145-162 cm tall. The levels of progesterone, 17-hydroxyprogesterone, and adrenocorticotropic hormone (ACTH) of the 3 patients were relatively high, and that of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) of the 3 patients were low. Testosterone level of 1 patient was significantly elevated, and that of the other 2 patients was below the lower limit of normal range. Testicular ultrasound showed heterogeneous hyperechoic masses in both testes. CT of the adrenal glands showed bilateral adrenal enlargement with mass. All 3 patients were treated with dexamethasone. After 4-96 months of follow-up, 17-hydroxyprogesterone level was kept above the median normal level. One of the patients got married and had a baby after treatment. The sizes of adrenal hyperplasia and testicular masses reduced to various degrees with the change of the testicular masses being proportional to that of adrenal hyperplasia. Conclusions: Patients with 21-OHD are prone to have TART, leading to the impaired testicular function. Early glucocorticold therapy is beneficial to the reduction of TART and restoration of testicular function.
- Published
- 2022
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21. [Mid-to-long term clinical outcomes and predictors after anterior cruciate ligament revision].
- Author
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Jiang YF, Wang J, Wang YJ, Liu J, Pei Y, Liu XP, Ao YF, and Ma Y
- Subjects
- Anterior Cruciate Ligament surgery, Humans, Knee Joint surgery, Reoperation, Treatment Outcome, Anterior Cruciate Ligament Injuries surgery, Anterior Cruciate Ligament Reconstruction
- Abstract
Objective: To assess the mid-to-long term clinical outcomes after anterior cruciate ligament (ACL) revision surgery and to analyze their predictors., Methods: The medical records of 235 patients undergoing ACL revision surgery between Jan. 2001 and Dec. 2015 at Department of Sports Medicine, Peking University Third Hospital were reviewed. Data were collected including demographic information, information related to revision surgery (time and cause of graft failure, date of revision surgery, surgical technique, combined injuries and management, etc .), as well as information related to primary ACL reconstruction (time, cause and mechanism of first-time ACL rupture, date of primary ACL reconstruction, surgical technique, combined injuries and management, etc .). Patients were followed up at least 2 years after revision surgery for clinical outcomes [Tegner score, Lysholm score, and International Knee Documentation Committee (IKDC) subjective knee score]. Post-revision surgeries on the involved knee and the contralateral knee joint were also documented. Multivariate regression model was used to analyze the predictors of clinical outcomes after ACL revision surgery., Results: A total of 166 (70.63%) patients were followed up at a mean of (4.44±2.40) years (2.03-14.63 years). Clinical outcomes improved significantly at the last follow-up from pre-operative level, with the Lysholm, Tegner, and IKDC scores improving from 70.51±21.25, 3.39±1.77, 63.78±15.04 to 88.64±14.36, 4.67±1.739, 80.23±13.31 ( P < 0.05), respectively. Three (1.81%) patients experienced infection while 39 (23.49%) patients underwent surgery after revision surgery during the follow-up. Compared with that those occurred during sports, graft failure that occurred during daily activities or due to surgical technical errors that led to poorer clinical outcomes, with the Lysholm, Tegner, and IKDC scores of 9.90 (95% CI : 1.49-18.31), 1.41 (95% CI : 0.10-2.72), 10.35 (95% CI : 0.17-20.54), and 8.53 (95% CI : 1.31-15.75), 1.28 (95% CI : 0.14-2.43), 9.39 (95% CI : 1.03-17.74) lower, respectively. Compared with antero-medial portal, transtibial technique for placement of the femoral bone tunnel showed poorer Lysholm scores of 11.18 (95% CI : 4.73-17.63, P =0.001). Concurrent repair of medial meniscus yielded higher IKDC scores of 11.06 (95% CI : 1.21-20.92, P =0.029) than those with intact medical meniscus. Other factors showed no significant effect., Conclusion: ACL revision surgery is able to restore knee stability and improve knee function. Graft failure caused by sports, concurrent repair of medical meniscus and antero-medial portal technique predicts better outcomes after revision surgery.
- Published
- 2021
22. Immigration and Oral Health in Older Adults: An Integrative Approach.
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Wu B, Mao W, Qi X, and Pei Y
- Subjects
- Acculturation, Aged, Humans, Oral Health, Oral Hygiene, Emigrants and Immigrants, Emigration and Immigration
- Abstract
The aim of this study was to develop an integrative framework on aging, immigration, and oral health. The methodology was a critical review that used immigration as a social determinant framework through which to evaluate its impact on the oral health of older immigrants. We reviewed recent empirical evidence on factors related to oral health in older immigrants. In a systematic search across multiple databases, we identified 12 eligible studies in this review. Among the eligible studies, most were conducted among East Asian immigrants (8 articles), followed by non-Hispanic White/European origin (2 articles), Mexican origins (1 article), and Iran and other Middle East regions (1 article). The research revealed knowledge gaps in the evidence base, including the dynamic relationship between acculturation and oral health, the role of environmental factors on oral health for immigrants, psychosocial stressors and their relationship with oral health, and oral health literacy, norms, and attitude to dental care utilization and oral hygiene practices. The development of the integrative framework suggests the pathways/mechanisms through which immigration exerts influences on oral health in later life. This provides opportunities for researchers, practitioners, and policy makers to gain greater insights into the complex associations between immigration and oral health among older adults.
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- 2021
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23. Early outcome and safety of bedaquiline-containing regimens for treatment of MDR- and XDR-TB in China: a multicentre study.
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Gao M, Gao J, Xie L, Wu G, Chen W, Chen Y, Pei Y, Li G, Liu Y, Shu W, Fan L, Wu Q, Du J, Chen X, Tang P, Xiong Y, Li M, Cai Q, Jin L, Mei Z, Pang Y, and Li L
- Subjects
- Adult, Antitubercular Agents administration & dosage, China epidemiology, Diarylquinolines administration & dosage, Drug Therapy, Combination, Female, Humans, Male, Middle Aged, Antitubercular Agents therapeutic use, Diarylquinolines therapeutic use, Tuberculosis, Multidrug-Resistant drug therapy, Tuberculosis, Multidrug-Resistant epidemiology
- Abstract
Objectives: Bedaquiline treatment significantly improves multidrug-resistant tuberculosis (MDR-TB) patient treatment outcomes. However, safety and efficacy data are lacking for bedaquiline used with background regimens to treat Chinese TB patients. Here, we describe our initial clinical experience for bedaquiline treatment of a large multicentre cohort of MDR-TB and extensively drug-resistant tuberculosis (XDR-TB) patients in China., Methods: Patients (177) received 24-week bedaquiline treatment combined with personalized anti-TB drug background regimens. As primary efficacy endpoints, times to initial sputum culture conversion were measured., Results: Of 177 MDR-TB patients completing the 24-week treatment course, sputum culture conversion occurred for 151/177 (85.3%), while 26 had unfavourable outcomes, including 3/177 (1.7%) deaths and 23/177 (13.0%) non-responders at treatment completion. The median time to sputum culture conversion was 4 (interquartile range 2-8) weeks. Conversion rates were 33/39 (84.6%, 95% confidence interval (CI) 73.3-95.9) for MDR-TB patients, 47/56 (83.9%, 95% CI 74.3-93.6) for pre-XDR-TB patients and 71/82 (86.6%, 95% CI 79.2-94.0) for XDR-TB patients. Multivariate analysis demonstrated that patients with low body mass index (odds ratio 7.356; 95% CI 2.652-20.401) were at significantly high risk of unfavourable outcomes, with serious adverse events noted in 15 (8.5%) patients, including six with corrected QT interval (QTc) prolongation times (>500 ms)., Conclusion: Bedaquiline, when included in background regimens for treatment of MDR-TB and XDR-TB patients in China, was safe and associated with a high rate of culture conversion., (Copyright © 2020 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.)
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- 2021
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24. [Clinical aspects of pregnancy-related lymphocytic hypophysitis].
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Li ZW, Yang GQ, Dou JT, Lyu ZH, Pei Y, Du J, Ba JM, Guo QH, and Mu YM
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- Adult, Female, Humans, Magnetic Resonance Imaging, Pituitary Gland, Pregnancy, Young Adult, Autoimmune Hypophysitis diagnosis, Hypopituitarism, Pituitary Diseases diagnosis
- Abstract
Objective: To analyze clinical features, diagnosis, treatment and prognosis of pregnancy-related lymphocytic hypophysitis (LyH). Methods: The clinical data of 16 cases diagnosed as pregnancy-related LyH at Chinese PLA General Hospital between October 2010 and November 2019 were reviewed. Results: Sixteen patients were included (aged 20-40 years). All patients' symptoms occurred from the last 2 months of pregnancy to 12 months postpartum, with 6 cases in the third trimester and 10 cases during postpartum, and all the patients were diagnosed after delivery. Six patients had lymphocytic adenohypophysitis (LAH), 4 patients had lymphocytic infundibuloneurohypophysitis (LINH), 4 patients had lymphocytic panhypophysitis (LPH), and 2 had lymphocytic hypothalamitis. Eight patients presented with symptoms of intracranial space-occupying lesions, 14 patients had symptoms of anteriorpituitary hormone deficiencies, 9 patients had central diabetes insipidus (CDI), and 2 had hyperprolactinemia. Pituitary MRI showed that the pituitary presented with diffuse enlargement, pituitary stalk thickening, disappearing of high-intensity signals in posterior pituitary and space-occupying lesions in the infundibulum of hypothalamus. Nine patients were treated with immunosuppressive agent, 3 patients alleviated the space-occupying effects after surgery, and 4 patients recovered spontaneously. Fourteen patients were followed up with a period of 3-98 months. Four patients had a relapse, 2 patients had a complete remission, and 12 patients needed long-term hormone replacement therapy. Conclusions: Clinical manifestations of pregnancy-related LyH are diverse. LyH should be suspected in pregnant or postpartum women with a sellar mass to avoid missed diagnosis or misdiagnosis. Immunosuppressant therapy is effective. Overall, LyH patients have a favorable prognosis.
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- 2020
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25. Melatonin activates autophagy via the NF-κB signaling pathway to prevent extracellular matrix degeneration in intervertebral disc.
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Chen F, Liu H, Wang X, Li Z, Zhang J, Pei Y, Zheng Z, and Wang J
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- Adolescent, Adult, Aged, Animals, Annulus Fibrosus surgery, Disease Models, Animal, Extracellular Matrix metabolism, Extracellular Matrix ultrastructure, Female, Humans, Intervertebral Disc metabolism, Intervertebral Disc ultrastructure, Intervertebral Disc Degeneration metabolism, Male, Microscopy, Electron, Transmission, Middle Aged, NF-kappa B metabolism, Nucleus Pulposus ultrastructure, Punctures, Rats, Signal Transduction, Young Adult, Antioxidants pharmacology, Autophagy drug effects, Extracellular Matrix drug effects, Intervertebral Disc drug effects, Intervertebral Disc Degeneration pathology, Melatonin pharmacology, NF-kappa B drug effects
- Abstract
Objective: This study investigated whether melatonin alleviates intervertebral disc degeneration (IVDD) by promoting autophagy through inhibiting the NF-κB signaling pathway., Methods: Magnetic resonance imaging (MRI), hematoxylin and eosin (H&E) staining and Safranin-O staining were used to measure disc degeneration in rat needle puncture IVDD models, and melatonin was injected intraperitoneally in the treated group to test its function. The expression of autophagy and extracellular matrix (ECM) degeneration related-markers were measured in the discs using immunohistochemistry. Transmission electron microscopy was used to evaluate the activation of autophagy in human nucleus pulposus (NP) tissues with different degenerated statuses. The expression of autophagy and disc degeneration related-markers were detected in NP cells by Western blot, RT-qPCR, and immunofluorescence analyses. NF-κB signaling pathway involvement was studied by lentivirus-mediated knockdown, Western blotting, and immunohistochemistry and immunofluorescence staining., Results: Melatonin prevented IVDD development in vivo and in vitro. Compared to non-degenerated disc tissues, degenerated human NP tissues showed a decrease in the autophagy-specific marker LC3B and the numbers of autophagosomes and autolysosomes, whereas the p62 level was increased; similar results were observed in rat IVDD models, indicating a negative correlation between autophagy and IVDD. Furthermore, both in vivo and in vitro studies found that melatonin application induced autophagy and reduced ECM disc degradation. Melatonin was also shown to regulate autophagy by inhibiting the NF-κB signaling pathway in vivo and vitro., Conclusion: This study indicates that melatonin prevents IVDD by promoting autophagy, indicating its possible therapeutic potential for controlling the progression of IVDD., (Copyright © 2020 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.)
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- 2020
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26. [A case of lymphocytic hypophysitis presenting as spontaneous resolution of a pituitary mass].
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Wang YJ, Du J, Pei Y, Chen K, Yang GQ, Wang XL, Guo QH, Lyu ZH, Ba JM, Dou JT, and Mu YM
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- Autoimmune Diseases pathology, Humans, Hypopituitarism, Inflammation diagnosis, Inflammation pathology, Magnetic Resonance Imaging, Autoimmune Diseases diagnosis, Autoimmune Hypophysitis diagnosis, Pituitary Gland pathology
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- 2020
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27. [Clinical efficacy and safety of apatinib combined with chemotherapy for osteosarcoma and soft tissue sarcoma with pulmonary metastasis].
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Li SL, Yang QK, Chen P, Zheng K, Wang W, Pei Y, and Zhang XJ
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- Adult, Bone Neoplasms pathology, Child, China, Humans, Sarcoma drug therapy, Sarcoma secondary, Soft Tissue Neoplasms pathology, Treatment Outcome, Antineoplastic Agents therapeutic use, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Bone Neoplasms drug therapy, Lung Neoplasms secondary, Osteosarcoma drug therapy, Osteosarcoma secondary, Pyridines therapeutic use, Soft Tissue Neoplasms drug therapy
- Abstract
Objective: To compare the clinical efficacy and drug safety between oral apatinib combined with conventional chemotherapy and conventional chemotherapy alone for the treatment of osteosarcoma and soft tissue sarcoma patients with pulmonary metastasis. Methods: Thirty-three osteosarcoma and soft tissue sarcoma patients with pulmonary metastasis who were treated in the Department of Bone and Soft Tissue Tumor Surgery, Cancer Hospital of China Medical University from January 2015 to December 2017 were enrolled in this study. Patients with osteosarcoma received methotrexate, adriamycin (ADM), cisplatin (CDDP), ifosfamide (IFO) sequential regimen; patients with soft tissue sarcoma were treated with IFO and ADM regimen. Eighteen of these patients received an additional oral dose of apatinib. The patients were followed up regularly for changes in primary tumors and metastases, adverse reactions and prognosis. Results: Before treatment, the maximum diameter of pulmonary metastases in patients of apatinib group and routine treatment group were (4.46±1.70) cm and (4.53±2.00) cm, respectively, without significant difference ( P =0.909). After treatment, the maximum diameter of pulmonary metastases in patients of apatinib group was (1.46±1.39) cm, significantly smaller than (3.02±1.20) cm of routine treatment group ( P =0.002). After treatment, the maximum diameter of the primary lesions in the apatinib group and the conventional treatment group median decreased 0.31 cm and 0.12 cm, respectively, without significant difference ( P =0.542). After treatment, the maximum diameter of the lung metastases in the apatinib group median decreased 0.59 cm, significantly more than 0.18 cm of the conventional treatment group ( P =0.027). The median progression-free survival (PFS) was 9.4 months in the 33 patients. The median PFS was 9.6 months and 8.3 months in the apatinib group and the conventional treatment group, respectively, without significant difference ( P =0.593). Specific adverse reactions both occurred in apatinib group and routine treatment group, mainly including oral mucosal reactions and digestive tract reactions (including nausea, vomiting and diarrhea). Conclusions: Apatinib can effectively reduce the volume of primary and metastatic lesions in patients with bone and soft tissue sarcoma accompanied by lung metastasis without reducing the survival rate or causing uncontrollable adverse reactions. The safety and clinical efficacy of apatinib are significant.
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- 2019
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28. [Differences of brain functional alterations between subtypes of Cushing's syndrome patients].
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Liu DD, Zhou W, Li PL, Zhang JL, Chen W, Gu WJ, Pei Y, Du J, Zang L, Ba JM, Lü ZH, Mu YM, Shan BC, Zhang YL, Ma L, and Dou JT
- Subjects
- Adrenocorticotropic Hormone, Adult, Brain, Depression, Female, Humans, Hydrocortisone, Male, Middle Aged, Cushing Syndrome
- Abstract
Objective: To compare the differences of brain functional damage of subtypes of patients with Cushing's syndrome (CS). Methods: A total of 11 adrenocorticotropic hormone (ACTH)-dependent CS patients and 29 ACTH-independent CS patients were recruited from Chinese PLA General Hospital between September 2015 and March 2017 with confirmed CS. The psychiatric scales and brain task functional magnetic resonance imaging (fMRI) were evaluated. Results: A total of 40 patients (34 females, 6 males) with a mean age of (39.20±12.10) years and a median education level of 12 (9, 16) years were enrolled. ACTH-dependent patients had significantly worse performance than the ACTH-independent patients in response to the depression evaluation (64.6±6.1 vs 56.2±12.8, P= 0.008), positive emotion (17.8±4.2 vs 24.3±7.2, P= 0.008) and CS life quality [31(29,33) vs 42(29,51), P= 0.040]. In the reaction to positive target pictures, ACTH-dependent CS patients showed stronger activation in left superior temporal gyrus compared with patients in ACTH-independent group, while the activation degree of their bilateral dorsal anterior cingulate cortex, bilateralsuperior frontal gyrus and left middle frontal gyrus was much worse. In the reactions to negative target pictures, ACTH-dependent CS patients had weaker activation in bilateral cerebellum, left superior frontal gyrus, left middle frontal gyrus, left precuneus and right postcentral gyrus, compared with patients in the ACTH-independent CS group ( P< 0.01, AlphaSim corrected). The activation degree of some regions whose brain function was different between the two groups was correlated to the cortisol level, ACTH level, 24 h urinary free cortisol (UFC) level, depression evaluation and negative emotion assessment (all P< 0.05). Conclusions: The severity of the depression and the life quality of patients in ACTH-dependent group are worse than ACTH-independent CS patients. The brain function of ACTH-dependent CS patients is much weaker.
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- 2019
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29. Transcatheter aortic valve replacement with balloon-expandable valve : Analysis of initial experience in China.
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Shen Y, Zhang H, Zhang L, Li H, Mao H, Pei Y, Jing Z, and Lu Q
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- Aged, Aged, 80 and over, Aortic Valve, China, Female, Humans, Male, Prosthesis Design, Retrospective Studies, Treatment Outcome, Aortic Valve Stenosis therapy, Heart Valve Prosthesis, Heart Valve Prosthesis Implantation, Transcatheter Aortic Valve Replacement
- Abstract
Background: Transcatheter aortic valve replacement (TAVR) is widely applied for the treatment of severe aortic stenosis (AS) in developed countries; however, in China, it is still in the early stage of utilization. On the basis of previous studies, this work explored the feasibility of TAVR in patients with severe AS in China and analyzed the cause of death in four cases., Methods: This retrospective study included 20 patients who had severe AS and underwent TAVR with a balloon-expandable system (Edwards SAPIEN XT) in our hospital from January 2011 to June 2016. The valve and heart functions of 16 survivors before and after the TAVR procedure were compared. TAVR endpoints, device success, and adverse events were assessed according to the definitions of the Valve Academic Research Consortium-2 (VARC-2)., Results: There were 13 male and seven female patients aged 65-81 years (average, 73.15) who underwent TAVR. The TAVR approach was transfemoral in 19 patients and transapical in one patient. Four patients died (two of coronary artery occlusion and two of aortic annulus rupture) during the TAVR procedure or shortly after; six patients had mild paravalvular leakage, and the rest of the patients showed a significant improvement in cardiac function. During the follow-up period (2-62 months), one patient died of lung cancer 13 months after the TAVR procedure., Conclusion: TAVR with a balloon-expandable system is safe and effective and can be used for patients with severe AS in China. It requires careful patient selection and preoperative assessment so as to reduce the 30-day postoperative mortality rate.
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- 2018
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30. Protective Effects of an Obesity-Associated Polymorphism (CDKAL1 rs9356744) on Prediabetes: The Cardiometabolic Risk in Chinese (CRC) Study.
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Liang J, Zhu Y, Liu XK, Qiu QQ, Sun YT, Wang Y, Pei Y, Yang MQ, and Qi L
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- Adult, Aged, Body Mass Index, China, Female, Humans, Male, Middle Aged, Polymorphism, Genetic genetics, Protective Factors, Diabetes Mellitus, Type 2 blood, Diabetes Mellitus, Type 2 genetics, Obesity genetics, Prediabetic State blood, Prediabetic State genetics, tRNA Methyltransferases genetics
- Abstract
Background: Obesity is strongly associated with insulin resistance and elevated plasma glucose levels. The rs9356744 polymorphism in the CDKAL1 gene is associated with body mass index (BMI) only in East Asians. Here, we examined the effect of the rs9356744 polymorphism on glucose-related traits and prediabetes in Chinese adults., Methods: A total of 2 357 participants were enrolled from the Cardiometabolic Risk in Chinese (CRC) Study, including 499 persons with prediabetes, 204 persons with type 2 diabetes, and 1 654 normoglycemic controls. The rs9356744 polymorphism in CDKAL1 was genotyped and analyzed in all participants., Results: Despite the positive relationship between obesity and glucose traits, the T allele of rs9356744, which is associated with a predisposition to obesity, was correlated with lower levels of 2-h oral glucose tolerance test (OGTT) plasma glucose (2hPG) (β=- 0.2104 and P =0.0233), glycated hemoglobin (HbA1c) (β=- 0.0551 and P =0.0298) and higher levels of homeostasis model of assessment β-cell function (HOMA-B) (β=5.282 and P =0.0424). After further adjustment for BMI, the levels of HOMA-B maintained a similar increased trend across rs9356744 genotype (β=3.277 and P =0.1958). In stratified analyses, the associations of rs9356744 with 2hPG and HbA1c were significant for individuals with a low BMI. Moreover, an antagonism action of BMI and rs9356744 on 2hPG ( P for interaction=0.0055) was observed. In addition, we found a protective effect of rs9356744 on prediabetes., Conclusions: The CDKAL1 rs9356744 T allele associated with a predisposition to obesity showed a protective effect on HbA1c, 2hPG, and prediabetes. BMI was mediator of the association between the genetic variant and HbA1c, 2hPG, and prediabetes., Competing Interests: Jun Liang, Yan Zhu, Xue-Kui Liu, Qin-Qin Qiu, Yu-Ting Sun, Yu Wang, Ying Pei, and Man-Qing Yang declare that they have no conflict of interest., (© Georg Thieme Verlag KG Stuttgart · New York.)
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- 2018
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31. [Clinical features and prognosis of 18 cases of primary lymphocytic hypophysitis].
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Wei Q, Yang GQ, Li YJ, Zang L, Pei Y, Du J, Gu WJ, Ba JM, Lü ZH, Dou JT, Mu YM, and Lu JM
- Subjects
- Female, Humans, Magnetic Resonance Imaging, Male, Pituitary Diseases, Pituitary Gland, Pregnancy, Prognosis, Autoimmune Hypophysitis
- Abstract
Objective: To analyze clinical features, prognosis and treatment of lymphocytic hypophysitis (LYH). Methods: The clinical data, treatments and outcomes of 18 cases diagnosed as LYH at Chinese PLA General Hospital between January 2001 and July 2017 was respectively reviewed. Results: Eighteen patients with histology-proven LYH (13 females and 5 males ) were identified. All lymphocytic adenohypophysitis (LAH) were females( n =6), two of whom were associated with pregnancy. Eleven patients (6 females and 5 males) had lymphocytic panhypophysitis (LPH) and one(female) had hypothalamitis. Pre-treatment evaluation revealed that 11 patients presented with symptoms of intracranial space-occupying lesions, 12 patients had symptoms of anterior pituitary hormone deficiencies, and 12 patients had central diabetes insipidus (CDI). All patients had space-occupying lesions on magnetic resonance imaging (MRI), which were symmetrically enlarged and homogenously enhanced with or without pituitary stalk thickening. Before or after surgery, 11 patients received immunosuppressant therapy or radiotherapy to alleviate space-occupying effect. After 4-204 months follow-up, 5 patients had a relapse and received immunosuppressants, radiotherapy or surgery to achieve remission. Full recovery (both symptomatic and radiographic) was seen in 6 patients, and 11 patients maintained stable replacement therapy. Conclusions: LYH presents with acute space-occupying effects such as headache, visual disturbances, hypopituitarism, CDI and mild hyperprolactinemia, especially with characteristic radiographic manifestations. Usually, surgery reliably establishes diagnosis, and immunosuppressant therapy is a necessity. On the whole, LYH has a good prognosis.
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- 2018
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32. [Clinical characteristics of 128 hospitalized patients with syndrome of inappropriate antidiuretics of different etiologies].
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Chen G, Li LL, Sun J, Gu WJ, Jin N, Yan WH, Chen K, Du J, Wang XL, Zang L, Pei Y, Guo QH, Yang GQ, Ba JM, Lyu ZH, Lu JM, Mu YM, and Dou JT
- Subjects
- Central Nervous System Diseases, Humans, Hyponatremia pathology, Inappropriate ADH Syndrome complications, Inappropriate ADH Syndrome pathology, Neoplasms pathology, Thorax, Central Nervous System pathology, Hyponatremia diagnosis, Inappropriate ADH Syndrome diagnosis, Inappropriate ADH Syndrome etiology, Neurophysins, Protein Precursors, Vasopressins
- Abstract
Objective: To summarize and analyze the clinical features and etiologies in hospitalized patients with syndrome of inappropriate antidiuretics (SIAD) during the past 25 years. Methods: All data of 128 patients with SIAD admitted to Chinese PLA General Hospital since January 1991 to January 2016 were collected. SIAD was diagnosed based on the 1957 criterion. Results: (1) The most frequent causes of increased inappropriate secretion of vasopressin were malignant tumors, lung diseases (e. g. pneumonia), and central nervous system diseases, in which malignant tumors accounted for 38.28% of the SIAD. (2) During the past 25 years, the proportion of malignant diseases declined from 4/7 to 35.29%, while, the proportion of pulmonary infection increased from 1/7 to 35.29% ( P <0.05). (3) The patients with malignant tumors had the lowest serum sodium and serum osmolality among all SIAD patients. (4) CT scan had a high diagnostic value for chest and brain detection. (5) Among three SIAD subjects with unknown reasons at onset, two were diagnosed with small cell lung cancer and one with gastric cancer during follow-up. Conclusion: The etiology of SIAD is complex and it could be attributed to multifarious etiological factors. Malignant tumors account for the largest proportion of all patients, and pulmonary infection was ranked in second place. Cautions on tumors have to be taken when serum sodium of a SIAD patient is below 118.1 mmol/L.
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- 2017
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33. Vertebral fracture in postmenopausal Chinese women: a population-based study.
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Cui L, Chen L, Xia W, Jiang Y, Cui L, Huang W, Wang W, Wang X, Pei Y, Zheng X, Wang Q, Ning Z, Li M, Wang O, Xing X, Lin Q, Yu W, Weng X, Xu L, and Cummings SR
- Subjects
- Absorptiometry, Photon, Aged, Aged, 80 and over, Bone Density physiology, China epidemiology, Exercise physiology, Female, Femur Neck physiopathology, Humans, Lumbar Vertebrae physiopathology, Middle Aged, Osteoporosis, Postmenopausal complications, Osteoporosis, Postmenopausal physiopathology, Osteoporotic Fractures etiology, Osteoporotic Fractures physiopathology, Prevalence, ROC Curve, Risk Factors, Spinal Fractures etiology, Spinal Fractures physiopathology, Osteoporosis, Postmenopausal epidemiology, Osteoporotic Fractures epidemiology, Spinal Fractures epidemiology
- Abstract
In a random sample of postmenopausal Chinese women, the prevalence of radiographic vertebral fractures increased from 13% between ages 50 and 59 to over 50% after age 80 years. A model with seven clinical risk factors predicted the probability of vertebral fractures as well with as without BMD and better than a model with only three risk factors. More than half an hour of outdoor activity per day might correlate with lower risk of vertebral fracture in this population., Introduction: We aimed to describe the prevalence and develop a model for prediction of radiographic vertebral fractures in a large random sample of postmenopausal Chinese women., Methods: We enrolled 1760 women from an age-stratified random sample of postmenopausal women in Beijing, China. The presence of vertebral fracture was assessed by semi-quantitative grading of lateral thoracolumbar radiographs, risk factors by interview, bone mineral density (BMD) of the proximal femur and lumbar spine by dual x-ray absorptiometry (DXA), and markers of bone turnover from a fasting blood sample. Associations of these factors were analyzed in logistic models and discrimination by areas of receiver operating characteristics curves (AUC)., Results: The prevalence of vertebral fracture, ranged from 13.4% ages 50 to 59 years old to 58.1% at age 80 years or older. Older age, a history of non-vertebral fracture, lower femoral neck BMD T-score, body mass index (BMI), height loss, housework, and less than half an hour of outdoor activity were significantly associated with increased probability of having a vertebral fracture. A model with those seven factors had a similar AUC with or without BMD and performed better than a simple model with three factors., Conclusion: This study is from a true random sample of postmenopausal women in urban China with high response rate. The prevalence of vertebral fractures in postmenopausal women in Beijing increases from 13% under age 60 to over 50% by age 80 years. A model with seven clinical risk factors with or without BMD is better than simple models and may guide the use of spine x-rays to identify women with vertebral fractures. More than half an hour of outdoor activity might correlate with lower risk of vertebral fracture in this population.
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- 2017
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34. Mutational analysis of HOXA10 gene in Chinese patients with cryptorchidism.
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Cheng Z, Wang M, Xu C, Pei Y, Liu JC, Huang H, He D, and Lu P
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- Adolescent, Asian People genetics, Child, Child, Preschool, China, DNA Mutational Analysis, Genetic Association Studies, Genetic Predisposition to Disease, Homeobox A10 Proteins, Humans, Infant, Male, Mutation, Cryptorchidism genetics, Homeodomain Proteins genetics
- Abstract
Cryptorchidism is one of the most common congenital anomalies and affects 2-4% of full-term new born boys. Its aetiology is poorly understood at present. HOXA10 plays a pivotal role in regulation of testicular descent. Male mice mutant for Hoxa10 exhibit unilateral or bilateral cryptorchidism as a result of impaired development of the gubernaculums. In this study, we performed mutation analysis of HOXA10 gene in a cohort of 98 cryptorchid patients. And we found a mutation (N27K) in a boy with unilateral cryptorchidism. The mutation was not detected in 106 healthy controls. Both in silico analyses and functional studies showed that the mutation affected the function of HOXA10. The results demonstrated that mutation in HOXA10 gene contributes to the pathogenesis of cryptorchidism, but may not be a common cause., (© 2016 Blackwell Verlag GmbH.)
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- 2017
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35. Correction: Identification of Novel Pre-Erythrocytic Malaria Antigen Candidates for Combination Vaccines with Circumsporozoite Protein.
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Speake C, Pichugin A, Sahu T, Malkov V, Morrison R, Pei Y, Juompan L, Milman N, Zarling S, Anderson C, MacDonald NJ, Wong-Madden S, Wendler J, Ishizuka A, MacMillen ZW, Garcia V, Kappe SH, Krzych U, and Duffy PE
- Abstract
[This corrects the article DOI: 10.1371/journal.pone.0159449.].
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- 2016
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36. Neddylation controls basal MKK7 kinase activity in breast cancer cells.
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Zhu T, Wang J, Pei Y, Wang Q, Wu Y, Qiu G, Zhang D, Lv M, Li W, and Zhang J
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- Breast Neoplasms enzymology, Breast Neoplasms metabolism, Cell Line, Tumor, Cell Proliferation, Epithelial-Mesenchymal Transition, Humans, JNK Mitogen-Activated Protein Kinases metabolism, Molecular Chaperones metabolism, NEDD8 Protein, Nuclear Pore Complex Proteins metabolism, Phenotype, Phosphorylation, Ubiquitin-Protein Ligases metabolism, Breast Neoplasms pathology, MAP Kinase Kinase 7 metabolism, Ubiquitins metabolism
- Abstract
The c-Jun NH2-terminal protein kinase (JNK) pathway has been implicated in mammary tumor development. However, the molecular mechanisms regulating JNK activity in breast cancer cells remain unclear. Here, we report that the inhibition of ubiquitination-like post-translational modification neddylation through different strategies results in enhanced basal JNK phosphorylation in human breast cancer cells. The upregulation of basal JNK phosphorylation upon neddylation inhibition is independent of the deneddylation of Cullins, the well-characterized neddylation substrates. Since augmented basal JNK phosphorylation via ectopic MKK7 expression impedes proliferation and the epithelial-to-mesenchymal transition (EMT) phenotype, the neddylation system might contribute to mammary tumor development partially through limiting basal JNK phosphorylation. Further exploration reveals that MKK7, a JNK-specific MAP2K, undergoes neddylation in human breast cancer cells. MKK7 co-precipitates with a fragment of Ran-binding protein 2 (RanBP2), a large multimodular and pleiotropic protein that has been recognized as a SUMO E3 ligase. Knockdown of RanBP2 attenuates MKK7 neddylation and augments basal JNK phosphorylation without affecting the neddylation of Cullins, whereas ectopic expression of a RanBP2 fragment possessing SUMO E3 activity (RanBP2ΔFG) manifests the opposite effects. In vitro neddylation assays confirm that RanBP2ΔFG works as the neddylation E3 ligase for MKK7. The basal kinase activity of endogenous MKK7 increases upon RanBP2 knockdown but decreases upon the ectopic expression of RanBP2ΔFG. Furthermore, purified MKK7 shows reduced basal kinase activity after in vitro neddylation by RanBP2ΔFG. Consistently, RanBP2 knockdown leads to reduced proliferation and impaired EMT phenotype in human breast cancer cells and the effects of RanBP2 knockdown are reversed by simultaneous MKK7 knockdown. Taken together, our data suggest that MKK7 undergoes neddylation in human breast cancer cells, which limits its basal kinase activity.
- Published
- 2016
- Full Text
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37. Serum uric acid and non-alcoholic fatty liver disease in non-hypertensive Chinese adults: the Cardiometabolic Risk in Chinese (CRC) study.
- Author
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Liang J, Pei Y, Gong Y, Liu XK, Dou LJ, Zou CY, Qiu QQ, Yang MQ, and Qi L
- Subjects
- Adult, Asian People, Biomarkers blood, China, Female, Humans, Hypertension blood, Male, Risk Factors, Non-alcoholic Fatty Liver Disease blood, Uric Acid blood
- Abstract
Objective: The causal relationship between serum uric acid (SUA) level and non-alcoholic fatty liver disease (NAFLD) has not yet been clarified. The objective of the study was to determine the association between SUA and NAFLD, as well as assess the interactions between SUA and other metabolic risk factors regarding NAFLD., Patients and Methods: The study samples related to a community-based health examination survey conducted in Central China. Initially, a total of 24,878 patients with medical examination were included. After excluding the individuals with confounding factors, the remaining 21,798 subjects with biomarkers available were included in the present study., Results: The data show that the risk of NAFLD significantly increased with the elevated SUA levels. Further adjustments for sex, age, and other confounding metabolic factors did not change the increasing trend of NAFLD risk. The odds ratios [ORs, 95% confidence interval (CI)] of NAFLD across the increasing quintiles of SUA were 1.00, 1,530 (1.174-1.995), 2.24 (1.714-2.886), 2.636 (2.019-3.441), and 3.714 (2.828-4.877) (p for trend < 0.0001). Also, significant interaction was found between SUA and prehypertension in relation to the NAFLD risk (p for interaction < 0.05)., Conclusions: SUA was significantly associated with NAFLD risk, independent of other metabolic risk factors, and SUA also had significant interaction with prehypertension regarding the risk of NAFLD.
- Published
- 2015
38. Woven silk fabric-reinforced silk nanofibrous scaffolds for regenerating load-bearing soft tissues.
- Author
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Han F, Liu S, Liu X, Pei Y, Bai S, Zhao H, Lu Q, Ma F, Kaplan DL, and Zhu H
- Subjects
- Amniotic Fluid cytology, Animals, Bombyx, Cell Line, Humans, Mechanical Phenomena, Nanofibers ultrastructure, Silk ultrastructure, Spectroscopy, Fourier Transform Infrared, Stem Cells cytology, Stress, Mechanical, Weight-Bearing, X-Ray Diffraction, Nanofibers chemistry, Regeneration, Silk chemistry, Tissue Engineering methods, Tissue Scaffolds chemistry
- Abstract
Although three-dimensional (3-D) porous regenerated silk scaffolds with outstanding biocompatibility, biodegradability and low inflammatory reactions have promising application in different tissue regeneration, the mechanical properties of regenerated scaffolds, especially suture retention strength, must be further improved to satisfy the requirements of clinical applications. This study presents woven silk fabric-reinforced silk nanofibrous scaffolds aimed at dermal tissue engineering. To improve the mechanical properties, silk scaffolds prepared by lyophilization were reinforced with degummed woven silk fabrics. The ultimate tensile strength, elongation at break and suture retention strength of the scaffolds were significantly improved, providing suitable mechanical properties strong enough for clinical applications. The stiffness and degradation behaviors were then further regulated by different after-treatment processes, making the scaffolds more suitable for dermal tissue regeneration. The in vitro cell culture results indicated that these scaffolds maintained their excellent biocompatibility after being reinforced with woven silk fabrics. Without sacrifice of porous structure and biocompatibility, the fabric-reinforced scaffolds with better mechanical properties could facilitate future clinical applications of silk as matrices in skin repair., (Copyright © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
39. Meta-analysis of associations between the TP53 Arg72Pro polymorphism with risk of head and neck carcinomas based on case-control studies.
- Author
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Ren WH, Jiang DK, Pei Y, Wang SQ, Yang XM, and Yu L
- Subjects
- Case-Control Studies, Genetic Association Studies, Humans, Carcinoma genetics, Head and Neck Neoplasms genetics, Mutation, Missense, Polymorphism, Single Nucleotide, Tumor Suppressor Protein p53 genetics
- Abstract
Genetic factors have been shown to play a role in the development of head and neck cancers (HNCs). However, studies investigating the association between the TP53 Arg72Pro polymorphism and HNCs susceptibility have yielded conflicting results. Hence, we performed a meta-analysis of all eligible studies (up to January 1, 2012) to derive a more precise estimation of this association in order to increase understanding of the possible risk factors of HNCs. Twenty-seven case-control studies involving 3966 cases and 4387 controls were included in our analysis. Overall, no evidence of association was observed between the TP53 Arg72Pro single nucleotide polymorphism (SNP) and the risk of HNCs in any genetic model (Arg/Arg vs Pro/Pro: odds ratio (OR) = 0.83, 95% confidence interval (CI): 0.65-1.06; Arg/Pro vs Pro/Pro: OR = 0.88, 95%CI= 0.70-1.10; Arg/Arg+Arg/Pro vs Pro/Pro: OR = 0.87, 95%CI= 0.70-1.09; Arg/Arg vs Arg/Pro+Pro/Pro: OR = 0.95, 95%CI= 0.82-1.11). Nevertheless, the TP53 Arg72Pro polymorphism shows diverse effects across different subtypes of HNCs. For example, there was a lack of association of this polymorphism with oral cavity cancer, whereas a significant association with nasopharyngeal cancer was observed. Results of this meta-analysis suggest that the TP53 Arg72Pro polymorphism might have different effects on the risk of various subtypes of HNCs.
- Published
- 2014
- Full Text
- View/download PDF
40. Robot-aided motion planning for knee joint rehabilitation with two robot-manipulators.
- Author
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Pei Y, Kim Y, Obinata G, Genda E, and Stefanov D
- Subjects
- Biomechanical Phenomena, Humans, Movement, Physical Therapy Modalities, Joint Diseases rehabilitation, Knee Joint physiopathology, Robotics
- Abstract
In this paper, we propose a simultaneous design method of motion and external force trajectories for knee joint rehabilitation based on the biomechanical analysis of the lower limb. In this method we assume to use two robots manipulators which provide forces and moments at shank and thigh. We developed a 7 degree of freedom musculoskeletal model of lower limb with 19 muscles. The valuation function of rehabilitation efficiency e has been maximized by Genetic Algorithm (GA) that refers to the musculoskeletal model and tunes motion trajectory of the robots and forces acting on the shank and thigh.
- Published
- 2013
- Full Text
- View/download PDF
41. Building a disease risk model of osteoporosis based on traditional Chinese medicine symptoms and western medicine risk factors.
- Author
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Zhou XH, Li SL, Tian F, Cai BJ, Xie YM, Pei Y, Kang S, Fan M, and Li JP
- Subjects
- Adult, Aged, Clinical Medicine statistics & numerical data, Cross-Sectional Studies, Data Mining, Female, Humans, Menopause, Middle Aged, Models, Statistical, Risk Factors, Support Vector Machine, Surveys and Questionnaires, Health Surveys statistics & numerical data, Medicine, Chinese Traditional, Models, Biological, Osteoporosis diagnosis, Osteoporosis epidemiology
- Abstract
In the Traditional Chinese Medicine (TCM) cross-sectional survey conducted by our team, we were interested in determining the risk factors of osteoporosis. To analyze this TCM study, we had to deal with three statistical problems: (1) a very large number of potential risk factors, (2) interactions among potential risk factors, and (3) nonlinear effects of some continuous-scale risk factors. To address these analytic issues, we used two data mining methods, support vector machine recursive feature elimination and random forest; to deal with the curse of high-dimensional risk factors, we applied another data mining technique of association rule learning to discover the potential associations among risk factors. Finally, we employed the generalized partial linear model (GPLM) to determine nonlinear effects of an important continuous-scale risk factor. The final GPLM model shows that TCM symptoms play an important role in assessing the risk of osteoporosis. The GPLM also reveals a nonlinear effect of the important risk factor, menopause years, which might be missed by the generalized linear model., (Copyright © 2012 John Wiley & Sons, Ltd.)
- Published
- 2012
- Full Text
- View/download PDF
42. Robot-aided rehabilitation task design for inner shoulder muscles.
- Author
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Pei Y, Kim Y, Obinata G, Genda E, and Stefanov D
- Subjects
- Biomechanical Phenomena, Computer Simulation, Humans, Models, Anatomic, Range of Motion, Articular, Muscle, Skeletal physiopathology, Rehabilitation methods, Robotics, Shoulder physiopathology, Task Performance and Analysis, Therapy, Computer-Assisted methods
- Abstract
In this paper, we propose a simultaneous design method of motion and external force trajectories for shoulder inner muscles in the robot-aided rehabilitation system, based on a biomechanical analysis of patient's body. The rehabilitation efficiency evaluation function was maximized by Genetic Algorithm (GA), where the structure of spline curves parameters are pre-defined, and the structural parameters are explored to design smooth rehabilitation motion and external force trajectories.
- Published
- 2012
- Full Text
- View/download PDF
43. Trajectory planning of a robot for lower limb rehabilitation.
- Author
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Pei Y, Kim Y, Obinata G, Hase K, and Stefanov D
- Subjects
- Computer Simulation, Humans, Models, Biological, Movement, Muscle Contraction, Leg physiopathology, Man-Machine Systems, Motion Therapy, Continuous Passive methods, Movement Disorders rehabilitation, Muscle, Skeletal physiopathology, Robotics methods, Therapy, Computer-Assisted methods
- Abstract
We introduce a method for lower-limb physical rehabilitation by means of a robot that applies preliminary defined forces to a patient's foot while moving it on a preliminary defined trajectory. We developed a special musculoskeletal model that takes into consideration the generated muscle forces of 27 musculotendon actuators and joint stiffness of the leg and allows the calculation of the motion trajectory of the robot and the forces that the robot needs to apply to the foot in each moment of the therapeutic exercise. Robotic treatment programs are customized for the individual patient by using a genetic algorithm (GA) that refers to the musculoskeletal model and calculates the parameters of the spline curves of the motion trajectory of the robot and forces acting on the foot.
- Published
- 2011
- Full Text
- View/download PDF
44. The promyelocytic leukemia zinc-finger gene, PLZF, is frequently downregulated in malignant mesothelioma cells and contributes to cell survival.
- Author
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Cheung M, Pei J, Pei Y, Jhanwar SC, Pass HI, and Testa JR
- Subjects
- Apoptosis genetics, Apoptosis physiology, Blotting, Western, Caspase 3 metabolism, Cell Cycle genetics, Cell Cycle physiology, Cell Survival genetics, Cell Survival physiology, Cyclin-Dependent Kinase Inhibitor p15 genetics, Cyclin-Dependent Kinase Inhibitor p16 genetics, Gene Dosage, Gene Expression Regulation, Neoplastic, Humans, Kruppel-Like Transcription Factors metabolism, Mesothelioma genetics, Mesothelioma metabolism, Mesothelioma pathology, Myeloid Cell Leukemia Sequence 1 Protein, Poly(ADP-ribose) Polymerases metabolism, Promyelocytic Leukemia Zinc Finger Protein, Proto-Oncogene Proteins c-bcl-2 metabolism, Reverse Transcriptase Polymerase Chain Reaction, Tumor Cells, Cultured, Tumor Suppressor Protein p14ARF genetics, Chromosome Deletion, Chromosomes, Human, Pair 9 genetics, Down-Regulation, Kruppel-Like Transcription Factors genetics
- Abstract
DNA copy number analysis was performed, using single-nucleotide polymorphism mapping arrays, to fine map genomic imbalances in human malignant mesothelioma (MM) cell lines derived from primary tumors. Chromosomal losses accounted for the majority of genomic imbalances. All 22 cell lines examined showed homozygous deletions of 9p21.3, centering at the CDKN2A/ARF and CDKN2B loci. Other commonly underrepresented segments included 1p36, 1p22, 3p21-22, 4q13, 4q34, 11q23, 13q12-13, 14q32, 15q15, 18q12, and 22q12, each observed in 55-90% of cell lines. Focal deletions of 11q23 encompassed the transcriptional repressor gene promyelocytic leukemia zinc finger (PLZF), which was validated by analysis of genomic DNA using real-time polymerase chain reaction (PCR). Semi-quantitative RT-PCR and immunoblot analysis revealed that PLZF is greatly downregulated in MM cell lines compared with non-malignant mesothelial cells. Ectopic expression of PLZF in PLZF-deficient MM cells resulted in decreased cell viability, reduced colony formation, as well as increased apoptosis, the latter based on results of various cell death assays and the observation of increased cleavage of caspase 3, PARP, and Mcl-1. These data indicate that deletions of PLZF are a common occurrence in MM and that downregulation of PLZF may contribute to MM pathogenesis by promoting cell survival.
- Published
- 2010
- Full Text
- View/download PDF
45. Occupational contact dermatitis (drug-induced medicamentosa): six case reports.
- Author
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Zou J, Liu L, Sun S, Zhao Y, Pei Y, and Liu G
- Subjects
- Acute Kidney Injury chemically induced, Adult, Chemical Industry, Drug Eruptions etiology, Epidermolysis Bullosa chemically induced, Fever chemically induced, Humans, Liver Failure, Acute chemically induced, Male, Naphthalenes toxicity, Quinolones toxicity, Dermatitis, Occupational etiology
- Abstract
We report six patients who had dermatitis after contacting certain chemical substances (one worker with contact 1-chloromethyl naphthalene and five with contact Hydroxy-phthalimide). They appeared like drug-induced erythma multiforme, bullosa epidermalysis from the point of contact gradually spreading to the whole body, combining high temperature, digestive system dysfunction, such as liver dysfunction etc. We can call this occupational drug-induced medicamentosa. The course of dermatosis diseases is 1 - 2 months. Hormone and sustaining treatment may be effective. We discuss chemistry substance, clinical presentation, diagnoses, treatment and relevant prognosis.
- Published
- 2007
- Full Text
- View/download PDF
46. Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.).
- Author
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An X, Zhang Q, Yan Y, Li Q, Zhang Y, Wang A, Pei Y, Tian J, Wang H, Hsam SL, and Zeller FJ
- Subjects
- Amino Acid Sequence, Base Composition, Cloning, Molecular, Gene Deletion, Genetic Markers, Glutens chemistry, Glutens metabolism, Molecular Sequence Data, Mutagenesis, Insertional, Phylogeny, Plant Proteins chemistry, Plant Proteins classification, Plant Proteins metabolism, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Prolamins, Protein Subunits chemistry, Protein Subunits genetics, Protein Subunits metabolism, Sequence Alignment, Triticum metabolism, Glutens genetics, Plant Proteins genetics, Triticum genetics
- Abstract
Three novel low molecular weight (LMW) glutenin subunits from cultivated einkorn (Triticum monococcum L., A(m)A(m), 2n = 2x = 14) were characterized by SDS-PAGE and molecular weights determined by MALDI-TOF-MS. Their coding genes were amplified and cloned with designed AS-PCR primers, revealing three complete gene sequences. All comprised upstream, open reading frame (ORF), downstream and no introns were present. The deduced amino acid sequences showed that all three genes, named as LMW-M1, LMW-M3 and LMW-M5, respectively, belonged to the LMW-i type subunits with the predicted molecular weight between 38.5206 and 38.7028 kDa. They showed high similarity with other LMW-i type genes from hexaploid bread wheats, but also displayed unique features. Particularly, LMW-M5 subunit contained an extra cysteine residue in the C-terminus except for eight conserved cysteines, which resulted from a single-nucleotide polymorphism (SNP) of the T-C transition, namely arginine --> cysteine substitution at position 242 from the N-terminal end. This is the first report that the LMW-i subunit contained nine cysteines residues that could result in a more highly cross-linked and more elastic glutenin suggesting that LMW-M5 gene may associates with good quality properties. In addition, a total of 25 SNPs and one insertions/deletions (InDels) were detected among three LMW-i genes, which could result in significant functional changes in polymer formation of gluten. It is anticipated that these SNPs could be used as reliable genetic markers during wheat quality improvement. The phylogenetic analysis indicated that LMW-i type genes apparently differed from LMW-m and LMW-s type genes and diverged early from the primitive LMW-GS gene family, at about 12.92 million years ago (MYA) while the differentiation of A(m) and A genomes was estimated at 3.98 MYA.
- Published
- 2006
- Full Text
- View/download PDF
47. Structural biology of RNA silencing and its functional implications.
- Author
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Patel DJ, Ma JB, Yuan YR, Ye K, Pei Y, Kuryavyi V, Malinina L, Meister G, and Tuschl T
- Subjects
- Bacterial Proteins chemistry, Bacterial Proteins genetics, Bacterial Proteins metabolism, Humans, MicroRNAs biosynthesis, Models, Biological, Models, Molecular, Peptide Initiation Factors chemistry, Peptide Initiation Factors genetics, Peptide Initiation Factors metabolism, Protein Structure, Tertiary, RNA, Messenger chemistry, RNA, Messenger genetics, RNA, Messenger metabolism, RNA, Small Interfering chemistry, RNA, Small Interfering genetics, RNA, Small Interfering metabolism, Ribonuclease III metabolism, Viral Proteins chemistry, Viral Proteins genetics, Viral Proteins metabolism, RNA Interference
- Abstract
We outline structure-function contributions from our laboratories on protein-RNA recognition events that monitor siRNA length, 5 -phosphate and 2-nucleotide 3 overhangs, as well as the architecture of Argonaute, its externally bound siRNA complex, and Argonaute-based models involving guide-strand-mediated mRNA binding, cleavage, and release.
- Published
- 2006
- Full Text
- View/download PDF
48. Mitochondrial DNA deletions in primate embryonic and adult stem cells.
- Author
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Gibson TC, Pei Y, Quebedeaux TM, and Brenner CA
- Subjects
- Animals, Cell Line, DNA Primers, Fertilization in Vitro, Genetic Vectors genetics, Polymerase Chain Reaction, Retroviridae, Stromal Cells chemistry, Bone Marrow Cells cytology, DNA, Mitochondrial genetics, Embryo, Mammalian cytology, Gene Deletion, Macaca mulatta genetics, Oocytes chemistry, Stem Cells chemistry
- Abstract
Mitochondrial DNA (mtDNA) mutations occur naturally in skeletal muscle fibers from aged rhesus macaques. In addition, mtDNA mutations have been observed in germinal vesicle oocytes from fertile monkeys. The goal of this study was to determine whether the rhesus macaque mitochondrial common deletion was present in oocytes and embryos generated by in-vitro embryo production (IVP), as well as in rhesus adult and embryonic stem cell lines. The rhesus common deletion was detected in IVP-generated embryos, three IVP-derived embryonic stem cell lines (ORMES 1, 2 and 7), one in-vivo-derived embryonic stem cell line (R4) and multiple passages of an adult bone marrow stromal cell (BMSC) line. Mitochondrial DNA from an adult adipose stromal cell (ATSC) line was compared with mtDNA from an immortalized line transfected with a retroviral vector expressing telomerase, ATSC-TERT. Multiple passages of the ATSC line harboured a dramatically higher level of the rhesus common deletion than the immortalized ATSC-TERT line. Accumulation of mtDNA mutations in oocytes, embryos and subsequent embryonic stem cell lines, as well as adult stem cell lines, may contribute to mitochondrial dysfunction, and thereby impair ATP production. The authors believe this information establishes a compelling argument for the parallel development of embryonic stem cell technology in non-human primates and humans.
- Published
- 2006
- Full Text
- View/download PDF
49. Ectopic expression of the human adenine nucleotide translocase, isoform 3 (ANT-3). Characterization of ligand binding properties.
- Author
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Carroll AK, Clevenger WR, Szabo T, Ackermann LE, Pei Y, Ghosh SS, Glasco S, Nazarbaghi R, Davis RE, and Anderson CM
- Subjects
- Adenosine Diphosphate metabolism, Animals, Atractyloside analogs & derivatives, Atractyloside chemistry, Atractyloside metabolism, Bongkrekic Acid metabolism, Cattle, Cell Line, Energy Metabolism, Gene Expression, Humans, In Vitro Techniques, Iodine Radioisotopes, Kinetics, Ligands, Mitochondria, Heart metabolism, Moths, Protein Binding, Recombinant Proteins genetics, Recombinant Proteins metabolism, Adenine Nucleotide Translocator 3 genetics, Adenine Nucleotide Translocator 3 metabolism, Mitochondrial Proteins genetics, Mitochondrial Proteins metabolism
- Abstract
The adenine nucleotide translocase (ANT) is a key component in maintaining cellular energy homeostasis, and has also been implicated in formation of the mitochondrial permeability transition pore. Human ANT-3 was cloned from a human heart cDNA library and expressed as a histidine-tagged fusion protein in the mitochondria of the Trichoplusia ni. cell line. Overexpression resulted in a concomitant decrease in the endogenous ANT content, allowing for the characterization of binding of known ANT ligands to the human protein. Binding affinities for bongkrekic acid (BKA), ADP, and atractyloside (ATR) were measured in mitochondria from the human ANT-3 expressing cell line, and compared to similar preparations from bovine heart mitochondria by use of a novel radioiodinated derivative of ATR. Binding to ANT-3 by the high affinity inhibitors BKA and ATR, as well as the lower affinity natural ligand ADP, was similar to that measured in bovine heart mitochondria, and to that previously reported for mammalian heart mitochondria. Characterizations such as these of human ANT isoforms may lead to drug development for enhanced mitochondrial function and cellular viability.
- Published
- 2005
- Full Text
- View/download PDF
50. Serum-free culture of rhesus monkey embryonic stem cells.
- Author
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Pei Y, Ma J, Zhang X, and Ji W
- Subjects
- Alkaline Phosphatase metabolism, Animals, Cell Differentiation physiology, Cell Line, Genetic Markers genetics, RNA, Messenger metabolism, Reverse Transcriptase Polymerase Chain Reaction, Totipotent Stem Cells enzymology, Cell Culture Techniques methods, Culture Media, Serum-Free, Macaca mulatta physiology, Totipotent Stem Cells cytology
- Abstract
Previous studies have shown that the maintenance and proliferation of undifferentiated rhesus monkey embryonic stem (rES) cells requires medium supplemented with fetal bovine serum (FBS). Due to the uncharacterized composition and variation in serum nature, the present study aimed to replace the serum-containing medium with a serum-free medium in the rES cell culture. The results showed that after the initial 48-h culture in the routinely used serum-containing medium, rES cells can grow and proliferate for a prolonged period in the serum-free medium composed of DMEM supplemented with a cocktail of BSA, IGF-1, TGF-alpha, bFGF, aFGF, estradiol, and progesterone. rES cells cultured in the serum-free medium maintained high level of alkaline phosphatase activity and OCT4 level. There was no indication of differentiation as judged by the marker gene expression of all three embryonic germ layers and trophoblast. In addition, serum-free culture would not affect the passage capacity and differentiation potential of rES cells. This work will facilitate the future study of induced differentiation of rES cells and other applications.
- Published
- 2003
- Full Text
- View/download PDF
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