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Numerous studies have investigated the risk factors of Alzheimer’s disease (AD); however, AD-risk factors related miRNAs were rarely reported. In this study, AD-risk factor related miRNAs of 105 Chinese individuals (45 AD patients and 60 cognitively normal controls) were investigated. The results showed that Hsa-miR-185-5p, Hsa-miR-20a-5p, and Hsa-miR-497-5p were related to AD and education, Hsa-miR-185-5p, Hsa-miR-181c-5p, Hsa-miR-664a-3p, Hsa-miR-27a-3p, Hsa-miR-451a, and Hsa-miR-320a were related to AD and depression. Target prediction of above miRNAs showed that these miRNAs were involved in the generation and clearance of amyloid-beta (Aβ), important molecules related to cognition, and disease-activated microglia response to AD. It is worth noting that Hsa-miR-185-5p was related to both education and depression, whose decreased expression pattern in AD patients was alleviated by education and enhanced by depression, and participates in Aβ generation and accumulation. Our results indicated that certain education and depression factors can contribute to AD progression by modulating miRNA expression, implying that preventive interventions might alter AD progression in Chinese patients.

Immune infiltration remains at a high level in clear cell renal cell carcinoma (ccRCC). It has been confirmed that immune cell infiltration in tumor microenvironment (TME) is intimately bound up with the progression and the clinical outcome of ccRCC. The prognostic model, developed based on different immune subtypes of ccRCC, has a predictive value in patients’ prognosis. RNA sequencing data, somatic mutation data of ccRCC and clinical information were acquired from the cancer genome atlas (TCGA) database. The key immune-related genes (IRGs) were selected and by univariate Cox, LASSO, and multivariate Cox regression analyses. Then the ccRCC prognostic model was developed. The applicability of this model was verified in the independent dataset GSE29609. Thirteen IRGs including CCL7, ATP6V1C2, ATP2B3, ELAVL2, SLC22A8, DPP6, EREG, SERPINA7, PAGE2B, ADCYAP1, ZNF560, MUC20, and ANKRD30A were finally selected and a 13-IRGs prognostic model was developed. Survival analysis demonstrated that when compared with the low-risk group, patients in the high-risk group had a lower overall survival (p

Bone fracture is one of the most common injuries in the human musculoskeletal system. This study was performed to investigate the effects of celastrol on bone wound healing in rats. Bone wound models of Sprague-Dawley rats were treated with low (10 μg/kg) and high (100 μg/kg) celastrol for 14 days. Serum calcium (Ca), phosphorus (P), and alkaline phosphatase (ALP) contents, bone mechanical properties, bone mineral density (BMD), and the levels of osteogenesis-related and inflammation-related proteins were assessed at the end of the experiments. Rats feeding with celastrol grew normally as control. Compared with model, celastrol administration significantly increased fracture strength, elastic load (0.12 vs 0.16 kg/m), bending energy (11.23 vs 14.23 n x mm), and BMD (0.49 vs 0.54 g/cm3), particularly at a high dose. Serum Ca (2.2 vs 2.7 mmol/L) and ALP (217.3 vs 245.8 IU/L) contents were significantly increased after a high dose celastrol administration, although P content did not change. Western blot analyses showed that OPG (0.72 vs 1.15) and COL-1 (0.20 vs 0.42) but not RUNX2 were upregulated significantly after celastrol administration, and IL-1α (0.82 vs 0.37), IL-6 (0.62 vs 0.28), MCP-1(0.68 vs 0.18), and VEGF (0.62 vs 0.42) were significantly downregulated, while IFN-γ was upregulated (0.29 vs 0.46). Our data demonstrate that celastrol effectively promotes the healing of bone wound in rats and may be further explored as a therapeutic agent to treat bone fracture.

During the COVID-19 pandemic, fresh live broadcasting has been widely present in consumers’ daily lives but has been scarcely examined in academic research. The major purpose of the current study is to examine how fresh live broadcast features (visibility, interactivity, and authenticity) impact consumers’ willingness to buy through consumers’ perceived value and perceived trust based on the stimulus–organism-response theory. A total of 307 Chinese webcast users participated in this study. The data were collected through an online questionnaire survey and analyzed by SPSS and Amos software. The findings discovered that the fresh live broadcast features positively impact consumers’ perceived utility value and trust, and the visibility and interactivity enhance the perceived hedonistic value of consumers. Moreover, perceived value and perceived trust mediate between fresh live broadcast features and consumers’ willingness to buy. This study emphasizes the important role of fresh live features and provides insight for fresh sellers to increase consumer willingness to buy based on the perspective of consumers’ perceived value and trust.

Although the number of studies on online reviews is growing, the impact of reviewer photo on consumer purchase decision-making has not yet been examined systematically. In particular, the underlying neural mechanisms have remained underexplored. Thus, the present study investigated whether and how reviewer photos affects consumers to make a purchase decision by using event-related potentials (ERPs). At the behavioral level, participants demonstrated a higher purchase rate with a shorter RT in situations with reviewer photos compared to situations without reviewer photos. Meanwhile, at the neural level, compared with situations without reviewer photos, situations with reviewer photos attracted more rapid attention resources at the early automatic processing phase, which induced a greater P2 amplitude, then mobilized more sustained attention allocation at the cognitive monitoring phase due to its evolutionary significance which elicited a more negative N2 amplitude, and finally resulted in a better evaluative categorization with higher motivational and emotional arousal due to its social presence which evoked a larger late positive potential (LPP) amplitude at the late elaborate cognitive processing phase. Those results illuminated the neural pathway of purchase decision-making when consumers were exposed in different conditions of reviewer photo. Moreover, the current study provided evidence for the underlying influence of reviewer photo on purchase decision-making in online shopping.

Numerous studies have investigated the risk factors of Alzheimer’s disease (AD), however, AD- risk factors related miRNAs were rarely reported. In this study, AD- risk factor related miRNAs of 119 Chinese individuals (47 AD patients and 62 cognitively normal controls) were investigated. The results showed that education, tea consumption, leisure activity, and social connection were low Body Mass Index (BMI), depression, and smoking were potentially risk factors for AD patients. Moreover, we screened exosome microRNAs (miRNAs) for the aforementioned lifestyle-related factors. Canonical correlation analyses revealed that our clinical observations were correlated with the miRNA expression profile for education, depression, leisure activity, and social connection. Furthermore, we predicted the targets of differentially expressed miRNAs and found that many of them were reported to be involved in the generation and clearance of Amyloid-beta (Aβ), important molecules related to cognition, and disease-activated microglia response to AD. Our results indicated that certain education and depression factors can contribute to AD progression by modulating miRNA expression, implying that preventive interventions might alter AD progression in Chinese patients.

Distant brand extension as an essential strategy of obtaining benefits was highly focused on the normal marketing practice and academic research. In the current study, we aim to recognize that how individuals with different levels of empathy respond to distant extensions under corporate social responsibility (CSR) and corporate competence (CC) associations to explore the corresponding neural mechanisms using event-related potentials (ERPs). We divided subjects into two groups involving a high empathy (HE) group and a low empathy (LE) group according to an empathy measure questionnaire. The subjects first faced a brand name following the CSR or CC association descriptions, and then, they were asked to evaluate the new product of brand by a five-point scale. Current results revealed that the participants of the HE group were more apt to accept the distant extension products than those of the LE group. Additionally, in the HE group, products from a brand with CSR associations were more acceptable than CC associations. Moreover, a larger N2 amplitude was elicited in the LE group than in the HE group. For the LE group, an augment N2 was found under CSR than CC associations, reflecting that LE consumers might perceive conflict when evaluating distant extensions and allocate more cognitive resources to deal with CSR information. At the later stage, the HE group showed a greater P3 than the LE group. For the HE group, an increased P3 was elicited under CSR than CC associations, suggesting that empathic individuals might show motivational salience and helping willingness toward distant extension products, especially under the CSR scenario. These results provide potential electrophysiological evidence for the positive impact of brand associations on the evaluation of distant brand extension in the case of subdividing different empathic individuals.

To evaluate the prognostic value of the percentage of high-grade patterns (micropapillary, solid, and complex glands) in early-stage lung adenocarcinoma (LUAD).A total of 1049 patients undergoing radical surgery with pathological T1-2N0M0 LUAD were screened retrospectively, and 191 patients were involved in the final analysis. Disease-free survival (DFS) was evaluated using the Kaplan-Meier curve and Cox regression analysis. The optimal cut-off value was determined using maximally selected rank statistics.The entire cohort was divided into quartile groups based on the percentage of high-grade patterns: Group 1 (≤ 30%), Group 2 (31-55%), Group 3 (56-85%), and Group 4 (≥ 86%). There were significant differences in smoking history (P = 0.041), EGFR mutations (P0.001), and ALK rearrangement (P = 0.010) between the four groups, but no significant differences in other clinicopathological features. Kaplan-Meier analysis showed that a higher percentage of high-grade patterns predicted worse DFS (P = 0.001), and multivariate analysis indicated that the percentage of high-grade patterns was an independent predictor (Group 2 vs. Group 1, HR = 2.136, P = 0.228; Group 3 vs. Group 1, HR = 3.355, P = 0.035; Group 4 vs. Group 1, HR = 5.147, P = 0.003, respectively). A cut-off value of 20% (P = 0.048) and 50% (P0.001) for high-grade patterns were tested, and both revealed a significant difference in distinguishing DFS between subgroups.The percentage of high-grade patterns is associated with the prognosis of early-stage invasive LUAD. A higher percentage indicates a worse prognosis.

Brand extension, as a marketing strategy, is frequently utilized by enterprises to produce new products. There exist several critical factors determining its success, such as brand reputation and perceived fit. The present study adopts the event-related potentials (ERPs) method to explore the underlying neural mechanism of the joint influence of the two factors on consumers’ evaluation of brand extension. Specifically, consumers were presented with a brand with corporate social responsibility (CSR) or corporate ability (CA) reputation, following attached to an extension product (high fit vs. low fit). And then, they were given a 5-point scale to report their acceptance intention (AI) toward the brand extension. Behavioral data showed a higher AI and a shorter reaction time for high fit in contrast to low fit conditions. For low fit conditions, consumers were more inclined to accept the extension product with a brand with CSR than CA reputation. Neurophysiologically, CSR reputation evoked a larger P2 amplitude and LPP amplitude than CA reputation. Moreover, the low fit conditions elicited a more positive LPP amplitude than the high fit conditions in the context of a brand with a CSR reputation. Yet, for a brand with a CA reputation, the effect of perceived fit was not found. These results may reflect early attention resources engagement and altruistic motivation at the late stage during brand extension evaluation. The findings provided neurological evidence for which of the two types of brand reputation (CSR vs. CA) have a more positive effect on brand extension.

There is a connection between the frontal negative slow wave (FNSW) and the arousal inhibition in the hedonic purchase context. To calculate the FNSW (400–800 ms), event-related potentials (ERPs) method was applied to depict the neural substrates on prudent and impulsive consumers’ behaviors within various states of promotion. Promotion types include the pure price promotion and the mixed promotion (a mixture of a charitable donation and a discount). Behaviorally, consumers response more quickly in the pure price promotion condition and they express a preference for the mixed promotion. More importantly, a larger FNSW emerged in the impulsive consumers than the prudent, suggesting that the former might tend to control their eagerness to consume hedonic items. Compared with the price promotion as the worse option, the mixed promotion as the better option caused more perceptual conflict, leading to an increase in N2 amplitude. It suggests that consumers incline to reject the worse offers. These results also reveal that people primarily have to search negative promotion information by their insight and subsequently impulsive consumers inhibit the responses to the promotion information. The method of ERPs and FNSW should be helpful for marketing researchers and professionals on hedonic consumption and sales promotion.

Since few reports have mentioned the role of FOSL1 in the radiotherapy sensitivity of glioma, this study would dig deep into this aspect. Cancer stem cells (CSCs) isolated by magnetic bead assay were identified by microscopy, qRT-PCR and western blot. The number of apoptotic cells was counted 72 h after X-ray irradiation to evaluate the sensitivity of cancer cells to radiotherapy. The effects of radiotherapy, FOSL1 and miR-27a-5p on basic cell functions were detected by functional experiments. The expressions of FOSL1, apoptosis-related genes and miR-27a-5p were detected by qRT-PCR and western blot as needed. The differential expression of FOSL1 and the effect of miR-27a-5p on survival rate were analyzed using GEPIA and UALCAN, respectively. FOSL1 was found highly expressed in glioma cells and patients. The appearance of spherical cells and high expressions of CSC-related markers indicated the successful isolation of CSC-like cells. The increment of X-ray dose enhanced the sensitivity of cancer cells to radiotherapy. Radiotherapy down-regulated cell viability and the expressions of FOSL1 and Bcl-2, but up-regulated cell apoptosis and the expressions of cleaved caspase-3 and Bax, which could be partially reversed by overexpressed FOSL1 or further enhanced by shFOSL1. MiR-27a-5p was highly expressed in in patients with glioma, which was associated with poor prognosis, while shFOSL1-inhibited miR-27a-5p expression enhanced the sensitivity of glioma stem cells to radiotherapy. In vivo experiments further verified the results obtained from in vitro experiments. Silent FOSL1 strengthened the radiosensitivity of glioma by down-regulating miR-27a-5p.

Continental strike-slip shear zones that may bear important information about the evolution of convergent tectonics often occur to accommodate plate convergence. When and how shearing along the shear zones responds to plate interactions, however, are often debated. In this study, we investigated the Oligocene–Miocene leucocratic dikes from the Ailao Shan–Red River shear zone, which was active during India-Eurasia plate convergence, to constrain the timing and mechanism of ductile shearing along the shear zone. The dikes are structurally grouped into pre-, syn-, and postkinematic types with respect to ductile shearing. Prekinematic dikes from ca. 41 to 30 Ma have low whole-rock 87Sr/86Sr(i) values (0.707–0.710), generally high εNd(t) values (–3.31∼–7.98), and variable εHf(t) values (–7.9∼+5.7). Their magma sources involved high thermal perturbation inducing partial melting of the lower crust, and contributions from the mantle that were possibly related to extensional collapse of the orogenic belt prior to tectonic extrusion of the Sundaland block. Syn- and postkinematic dikes from ca. 28 to 20 Ma dominantly have high whole-rock 87Sr/86Sr(i) (0.707–0.725) and low εNd(t) (–5.83 to –9.76) values, and either negative or positive zircon εHf(t) values (broadly in the range of –12 to + 7.6) for coeval but separate crustal magma sources. The results imply that major shearing accompanying retrograde metamorphism along the Ailao Shan–Red River shear zone was localized to crustal level. A synthesis of regional structural data suggests that Oligocene–Miocene shearing along the Ailao Shan–Red River shear zone and lateral tectonic extrusion of the Sundaland block proceeded in response to progressive India-Eurasia plate convergence. Distributed and inhomogeneous middle- to lower-crustal flow along the boundaries of and within the Sundaland block occurred during the tectonic extrusion.

In order to better understand how price and evaluation impact consumers' decision making, this research examined the neural bases underlying consumers' cognition of the two important clues when shopping online. An ERP experiment was conducted involving 19 Chinese students and the behavior data revealed that product price and evaluation significantly influenced their willingness to purchase mobile phones online, and the speed with which they made purchase decisions supported cue-diagnosis theory. Further, ERP results indicated three neural stages of decision-making: early automatic cognition with emotional valence and negativity bias effects elicited by product evaluation ratings, an evaluation stage exhibiting an attention, and a final stage featuring an evaluation-categorization pattern associated with pleasurable sensations. Specifically, those three neural stages of online decision-making were coded with corresponding event-related potentials in brain, that is, N1 and P2 in automatic cognition stage, P300 in evaluation phase, LPP in concluding phase. Hence, the research results reveal neurological effects of product price and evaluation on online purchases and deepen our comprehension of consumers' cognitive process.

The energy harvesting of a hydrokinetic turbine based on tandem flapping hydrofoils is investigated experimentally and numerically. The mechanisms with crankshafts and connecting rods are adopted to convert the oscillatory motions of the hydrofoils into rotary motions. The hind hydrofoil performs oscillatory motion in the vortex flow of the fore hydrofoil. The longitudinal spacing L x between the two hydrofoils is fixed while the phase difference e between the flapping motions of the two hydrofoils can be shifted from − π to π . The global phase shift Φ , which is the combination of L x , e and the Strouhal number S t , is adopted to describe the vortex interaction modes. The effects of S t and Φ on the energy harvesting have been tested. Experimental data show that the highest hydrodynamic efficiency of single hydrofoil is 25.2 % at S t = 0.235 . The optimum parameters for the energy harvesting of tandem hydrofoils have been found. The highest hydrodynamic efficiency is found at S t = 0.24 with Φ / 2 π ≈ 0.3 . Numerical simulations are performed to study the vortex interaction modes of these typical cases. The beneficial vortex pattern and the detrimental vortex pattern, which result in the highest and lowest efficiency of the turbine, have been investigated.

Aging is a primary risk factor for neurodegenerative diseases, such as Alzheimer's disease (AD). SIRT2, an NAD

For this paper, an experiment of low-pressure Argon inductively coupled plasma (ICP) was conducted in a closed chamber. Obvious $E$ – $H$ mode transition could be observed in the experiment. To solve the problem that microwave interference diagnosis can only measure the average electron density along the transmission path of the microwave, a new microwave interference diagnosis method based on fluid dynamics modeling is put forward to gain an accurate distribution of electron density. Using this new method, the spatial resolution of electron density could be improved. Finally, the electron density of low-pressure ICP was diagnosed both with this method and the Langmuir probe. The results revealed that the electron density increased with power and the peak value reached $7.8\times 10^{17}\text{m}^{-3}$ at the radio frequency power of 700 W. The consistency in the distribution trend of electron density that resulted from two different methods illustrates that the new method can provide a relatively accurate spatial distribution detail of electron density. The electron temperature was measured via the Langmuir probe and the results show that the temperature declines with power.

Adding electronegative gas to the inert gas is an important means of adjusting the plasma parameter distribution. In this paper, a fluid model of the Ar/O 2 inductivly coupled plasma (ICP) is used to investigate the parameter distribution, while the electron energy distribution function (EEDF) and the transport coefficients are obtained by the Boltzmann equation solver module to improve the accuracy. The spatial-temporal evolution of the electron density and the electron temperature of the ICP are obtained and the influence of the discharge power and the oxygen mole ratio on the distribution of the ICP parameters is compared. Additionally, the physical mechanism is researched using the diffusion-transport theory of the plasma. In order to verify the reliability of the model results, an experiment is carried out to diagnose the discharge parameters. The results of the simulation and the experiment are performed for different power and oxygen mole ratios and a good qualitative and numerical agreement is obtained, indicating that the inclusion of the simulation results in a high accuracy.

The basis of plasma stealth technology is the attenuation of electromagnetic waves by plasma. In this study, the calculation principle of the finite-difference time-domain (FDTD) method is introduced and a FDTD time–space coupling model of electromagnetic wave propagation in plasma is established. The time-domain variation characteristics of electromagnetic waves entering the plasma are analyzed. The plasma parameter distribution under different conditions obtained using the COMSOL fluid mechanics model is introduced into the FDTD model. The plasma reflectivity measurement experiment was carried out in a microwave anechoic chamber, and the influence of different experimental conditions on the plasma reflectivity was analyzed. The variation of reflectivity under different plasma parameter distributions is obtained. The results show that increasing electron density and plasma thickness and enhanced plasma distribution uniformity are beneficial for improving the attenuation effect of plasma on electromagnetic waves. These results provide a reference for the inductively coupled plasma parameter distribution in a closed quartz cavity, which provides a basis for the plasma to attenuate the electromagnetic waves.