1. Deficiency of calpain-6 inhibits primary ciliogenesis
- Author
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Bo Hye Kim, Gyuyeong Rah, Je Yeong Ko, Kyung Hyun Yoo, Sumin Oh, Jong Hoon Park, and Do Yeon Kim
- Subjects
Tubulin code ,Motility ,Acetylated α-tubulin ,Microtubules ,Biochemistry ,Ciliopathies ,Mice ,03 medical and health sciences ,Primary cilia ,Downregulation and upregulation ,Tubulin ,Microtubule ,Ciliogenesis ,Animals ,Hedgehog Proteins ,Cilia ,Molecular Biology ,0303 health sciences ,biology ,Calpain ,Cilium ,030302 biochemistry & molecular biology ,Acetylation ,Articles ,General Medicine ,Microarray Analysis ,Hedgehog signaling pathway ,Cell biology ,Calpain-6 ,NIH 3T3 Cells ,biology.protein ,mRNA microarray ,Signal Transduction - Abstract
The primary cilium is a microtubule-based structure projecting from a cell. Although the primary cilium shows no motility, it can recognize environmental stimuli. Thus, ciliary defects cause severe abnormalities called ciliopathies. Ciliogenesis is a very complex process and involves a myriad of components and regulators. In order to excavate the novel positive regulators of ciliogenesis, we performed mRNA microarray using starved NIH/3T3 cells. We selected 62 murine genes with corresponding human orthologs, with significantly upregulated expression at 24 h after serum withdrawal. Finally, calpain-6 was selected as a positive regulator of ciliogenesis. We found that calpain-6 deficiency reduced the percentage of ciliated cells and impaired sonic hedgehog signaling. It has been speculated that this defect might be associated with decreased levels of α-tubulin acetylation at lysine 40. This is the first study to report a novel role of calpain-6 in the formation of primary cilia. [BMB Reports 2019; 52(10): 619-624].
- Published
- 2019