11 results on '"Heng Huang"'
Search Results
2. Effect of receptor dimerization on membrane lipid raft structure continuously quantified on single cells by camera based fluorescence correlation spectroscopy.
- Author
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Heng Huang, M Fethullah Simsek, Weixiang Jin, and Arnd Pralle
- Subjects
Medicine ,Science - Abstract
Membrane bound cell signaling is modulated by the membrane ultra-structure, which itself may be affected by signaling. However, measuring the interaction of membrane proteins with membrane structures in intact cells in real-time poses considerable challenges. In this paper we present a non-destructive fluorescence method that quantifies these interactions in single cells, and is able to monitor the same cell continuously to observe small changes. This approach combines total internal fluorescence microscopy with fluorescence correlation spectroscopy to measure the protein's diffusion and molecular concentration in different sized areas simultaneously. It correctly differentiates proteins interacting with membrane fences from proteins interacting with cholesterol-stabilized domains, or lipid rafts. This method detects small perturbations of the membrane ultra-structure or of a protein's tendency to dimerize. Through continuous monitoring of single cells, we demonstrate how dimerization of GPI-anchored proteins increases their association with the structural domains. Using a dual-color approach we study the effect of dimerization of one GPI-anchored protein on another type of GPI-anchored protein expressed in the same cell. Scans over the cell surface reveal a correlation between cholesterol stabilized domains and membrane cytoskeleton.
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- 2015
- Full Text
- View/download PDF
3. Mitosis phase enrichment with identification of mitotic centromere-associated kinesin as a therapeutic target in castration-resistant prostate cancer.
- Author
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Kanishka Sircar, Heng Huang, Limei Hu, Yuexin Liu, Jasreman Dhillon, David Cogdell, Armen Aprikian, Eleni Efstathiou, Nora Navone, Patricia Troncoso, and Wei Zhang
- Subjects
Medicine ,Science - Abstract
The recently described transcriptomic switch to a mitosis program in castration-resistant prostate cancer (CRPC) suggests that mitotic proteins may be rationally targeted at this lethal stage of the disease. In this study, we showed upregulation of the mitosis-phase at the protein level in our cohort of 51 clinical CRPC cases and found centrosomal aberrations to also occur preferentially in CRPC compared with untreated, high Gleason-grade hormone-sensitive prostate cancer (P
- Published
- 2012
- Full Text
- View/download PDF
4. Associations of CFH polymorphisms and CFHR1-CFHR3 deletion with blood pressure and hypertension in Chinese population.
- Author
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Wei Gan, Johnna Wu, Ling Lu, Xu Xiao, Heng Huang, Fulong Wang, Jingwen Zhu, Liang Sun, Gang Liu, Yi Pan, Huaixing Li, Xu Lin, and Yan Chen
- Subjects
Medicine ,Science - Abstract
Dysregulation of the complement system has been linked to pathogenesis of hypertension. However, whether genetic changes of complement factor H (CFH) and its related genes are associated with hypertension is unknown. We genotyped three SNPs in the CFH gene cluster that are closely linked to age-related macular degeneration, namely rs1061170 (Y402H), rs2274700 (A473A) and rs7542235 (CFHR1-3Δ), and tested for their associations with blood pressure and hypertension risk in a population-based cohort including 3,210 unrelated Chinese Hans (50-70 years of age) from Beijing and Shanghai. We found that rs2274700 (A473A) and rs7542235 (CFHR1-3Δ) were both significantly associated with diastolic blood pressure (DBP) (β = 0.632-1.431, P≤0.038) and systolic blood pressure (SBP) (β = 1.567-4.445, P≤0.008), and rs2274700 (A473A) was associated with hypertension risk (OR [95%CI]: 1.175 [1.005-1.373], P = 0.048). Notably, the associations of rs2274700 (A473A) with DBP (P = 2.1×10(-3)), SBP (P = 8×10(-5)) and hypertension risk (P = 7.9×10(-3)) were significant only in the individuals with low CRP levels (
- Published
- 2012
- Full Text
- View/download PDF
5. High fat diet induces formation of spontaneous liposarcoma in mouse adipose tissue with overexpression of interleukin 22.
- Author
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Zheng Wang, Ling Yang, Yuhui Jiang, Zhi-Qiang Ling, Zhigang Li, Yuan Cheng, Heng Huang, Lingdi Wang, Yi Pan, Zhenzhen Wang, Xiaoqiang Yan, and Yan Chen
- Subjects
Medicine ,Science - Abstract
Interleukin 22 (IL-22) is a T-cell secreted cytokine that modulates inflammatory response in nonhematopoietic tissues such as epithelium and liver. The function of IL-22 in adipose tissue is currently unknown. We generated a transgenic mouse model with overexpression of IL-22 specifically in adipose tissue. The IL-22 transgenic mice had no apparent changes in obesity and insulin resistance after feeding with high fat diet (HFD). Unexpectedly, all the IL-22 transgenic mice fed with HFD for four months developed spontaneous tumors in epididymal adipose tissue. Histological analysis indicated that the tumors were well-differentiated liposarcomas with infiltration of inflammatory cells. IL-22 overexpression promotes production of inflammatory cytokines such as IL-1β and IL-10 and stimulates ERK phosphorylation in adipose tissue. Furthermore, IL-22 treatment in differentiated 3T3-L1 adipocytes could induce IL-1β and IL-10 expression, together with stimulation of ERK phosphorylation. Taken together, our study not only established a novel mouse model with spontaneous liposarcoma, but also revealed that IL-22 overexpression may collaborate with diet-induced obesity to impact on tumor development in mouse.
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- 2011
- Full Text
- View/download PDF
6. Effects of Helicobacter pylori treatment on the incidences of autoimmune diseases and inflammatory bowel disease in patients with diabetes mellitus
- Author
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Nai-Wei Sheu, Shu-Heng Huang, Deng-Chyang Wu, John Y. Kao, and Kun-Der Lin
- Subjects
Medicine ,Science - Abstract
Background Helicobacter pylori infection is known to decrease the incidences of autoimmune diseases and inflammatory bowel disease(IBD). Our aim was investigating the effect of H. pylori treatment in diabetes mellitus(DM) patients. Methods Adults with newly-diagnosed H. pylori infection or peptic ulcer disease(PUD) within the general population and DM population were identified from the National Health Insurance Research Database of Taiwan from 2000–2010. 79,181 patients were assigned to the 3 groups: general population with PUD without H. pylori treatment(PUD-HPRx in general population), DM patients with PUD without H. pylori treatment(PUD-HPRx in DM), and DM patients with PUD who received H. pylori treatment(PUD+HPRx in DM). Results Higher incidences of autoimmune diseases and IBD were observed in the PUD+HPRx in DM group than in the PUD-HPRx in general population and PUD-HPRx in DM groups (autoimmune diseases = 5.14% vs 3.47% and 3.65%; IBD = 5.60% vs 3.17% and 3.25%; PConclusions The results revealed that H. pylori treatment increased the incidences of autoimmune diseases and IBD and decreased the all-cause mortality in the DM group with PUD. The effect was more significant in younger patients. This finding assists in realizing the influence of H. pylori treatment in the DM population.
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- 2022
7. Effects of Helicobacter pylori treatment on the incidences of autoimmune diseases and inflammatory bowel disease in patients with diabetes mellitus
- Author
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Nai-Wei Sheu, Shu-Heng Huang, Deng-Chyang Wu, John Y. Kao, and Kun-Der Lin
- Subjects
Adult ,Multidisciplinary ,Helicobacter pylori ,Incidence ,Chronic Disease ,Diabetes Mellitus ,Humans ,Inflammatory Bowel Diseases ,Autoimmune Diseases ,Helicobacter Infections - Abstract
Background Helicobacter pylori infection is known to decrease the incidences of autoimmune diseases and inflammatory bowel disease(IBD). Our aim was investigating the effect of H. pylori treatment in diabetes mellitus(DM) patients. Methods Adults with newly-diagnosed H. pylori infection or peptic ulcer disease(PUD) within the general population and DM population were identified from the National Health Insurance Research Database of Taiwan from 2000–2010. 79,181 patients were assigned to the 3 groups: general population with PUD without H. pylori treatment(PUD-HPRx in general population), DM patients with PUD without H. pylori treatment(PUD-HPRx in DM), and DM patients with PUD who received H. pylori treatment(PUD+HPRx in DM). Results Higher incidences of autoimmune diseases and IBD were observed in the PUD+HPRx in DM group than in the PUD-HPRx in general population and PUD-HPRx in DM groups (autoimmune diseases = 5.14% vs 3.47% and 3.65%; IBD = 5.60% vs 3.17% and 3.25%; P Conclusions The results revealed that H. pylori treatment increased the incidences of autoimmune diseases and IBD and decreased the all-cause mortality in the DM group with PUD. The effect was more significant in younger patients. This finding assists in realizing the influence of H. pylori treatment in the DM population.
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- 2021
8. Multivariate multiple regression models of poly(ethylene-terephthalate) film degradation under outdoor and multi-stressor accelerated weathering exposures
- Author
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Devin A. Gordon, Laura S. Bruckman, Roger H. French, Wei-Heng Huang, and David M. Burns
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Atmospheric Science ,Multivariate statistics ,Light ,Polymers ,02 engineering and technology ,01 natural sciences ,7. Clean energy ,Random Allocation ,010104 statistics & probability ,Materials Testing ,Longitudinal Studies ,Materials ,Multidisciplinary ,Moisture ,Optical Materials ,Polyethylene Terephthalates ,Hydrolysis ,Physics ,Chemical Reactions ,Regression analysis ,Activation Energy ,021001 nanoscience & nanotechnology ,Chemistry ,Macromolecules ,Physical Sciences ,Regression Analysis ,Medicine ,0210 nano-technology ,Research Article ,Ultraviolet radiation ,Amorphous Solids ,Science ,Materials Science ,Soil science ,Weathering ,Cross-validation ,Meteorology ,Electromagnetic radiation ,Linear regression ,0101 mathematics ,Weather ,Photolysis ,Humidity ,Models, Theoretical ,Polymer Chemistry ,Gloss (optics) ,13. Climate action ,Multivariate Analysis ,Earth Sciences ,Glass ,Ultraviolet A - Abstract
Developing materials for use in photovoltaic (PV) systems requires knowledge of their performance over the warranted lifetime of the PV system. Poly(ethylene-terephthalate) (PET) is a critical component of PV module backsheets due to its dielectric properties and low cost. However, PET is susceptible to environmental stressors and degrades over time. Changes in the physical properties of nine PET grades were modeled after outdoor and accelerated weathering exposures to characterize the degradation process of PET and assess the influence of stabilizing additives and weathering factors. Multivariate multiple regression (MMR) models were developed to quantify changes in color, gloss, and haze of the materials. Natural splines were used to capture the non-linear relationship between predictors and responses. Model performance was evaluated via adjusted-R2 and root mean squared error values from leave-one-out cross validation analysis. All models described over 85% of the variation in the data with low relative error. Model coefficients were used to assess the influence of weathering stressors and material additives on the property changes of films. Photodose was found to be the primary degradation stressor and moisture was found to increase the degradation rate of PET. Direct moisture contact was found to impose more stress on the material than airbone moisture (humidity). Increasing the concentration of TiO2 was found to generally decrease the degradation rate of PET and mitigate hydrolytic degradation. MMR models were compared to physics-based models and agreement was found between the two modeling approaches. Cross-correlation of accelerated exposures to outdoor exposures was achieved via determination of cross-correlation scale factors. Cross-correlation revealed that direct moisture contact is a key factor for reliable accelerated weathering testing and provided a quantitative method to determine when accelerated exposure results can be made more aggressive to better approximate outdoor exposure conditions.
- Published
- 2018
9. Mitosis Phase Enrichment with Identification of Mitotic Centromere-Associated Kinesin As a Therapeutic Target in Castration-Resistant Prostate Cancer
- Author
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Armen Aprikian, Nora M. Navone, Jasreman Dhillon, Limei Hu, Eleni Efstathiou, Yuexin Liu, Wei Zhang, Kanishka Sircar, David Cogdell, Patricia Troncoso, and Heng Huang
- Subjects
Male ,Urology ,Cancer Treatment ,lcsh:Medicine ,Kinesins ,Mitosis ,Antineoplastic Agents ,Biology ,urologic and male genital diseases ,Transcriptome ,Prostate cancer ,Downregulation and upregulation ,medicine ,Humans ,Castration ,Molecular Targeted Therapy ,lcsh:Science ,Centrosome ,Gene knockdown ,Multidisciplinary ,lcsh:R ,Prostate Diseases ,Cancers and Neoplasms ,Prostatic Neoplasms ,medicine.disease ,Molecular biology ,Up-Regulation ,Gene expression profiling ,Genitourinary Tract Tumors ,Oncology ,Drug Resistance, Neoplasm ,Cancer research ,Androgens ,Kinesin ,Medicine ,lcsh:Q ,Neoplasm Grading ,Research Article - Abstract
The recently described transcriptomic switch to a mitosis program in castration-resistant prostate cancer (CRPC) suggests that mitotic proteins may be rationally targeted at this lethal stage of the disease. In this study, we showed upregulation of the mitosis-phase at the protein level in our cohort of 51 clinical CRPC cases and found centrosomal aberrations to also occur preferentially in CRPC compared with untreated, high Gleason-grade hormone-sensitive prostate cancer (P
- Published
- 2012
10. High fat diet induces formation of spontaneous liposarcoma in mouse adipose tissue with overexpression of interleukin 22
- Author
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Zhigang Li, Lingdi Wang, Yan(陈雁) Chen, Zheng Wang, Zhi-Qiang Ling, Yuhui Jiang, Xiaoqiang Yan, Heng Huang, Ling Yang, ZhenZhen(王甄真) Wang, Yuan Cheng, and Yi(潘怡) Pan
- Subjects
Genetically modified mouse ,Lifestyle Causes of Cancer ,Male ,medicine.medical_specialty ,Mouse ,Adipose tissue macrophages ,Immunology ,Adipose tissue ,Gene Expression ,lcsh:Medicine ,Inflammation ,Mice, Transgenic ,Biology ,Diet, High-Fat ,Proinflammatory cytokine ,Mice ,Model Organisms ,Internal medicine ,Bone and Soft Tissue Sarcomas ,Basic Cancer Research ,medicine ,Adipocytes ,Animals ,Phosphorylation ,Extracellular Signal-Regulated MAP Kinases ,lcsh:Science ,Nutrition ,Multidisciplinary ,Cancer Risk Factors ,Interleukins ,lcsh:R ,Interleukin ,Cancers and Neoplasms ,Animal Models ,Liposarcoma ,Epithelium ,Interleukin 10 ,Endocrinology ,medicine.anatomical_structure ,Oncology ,Adipose Tissue ,Immune System ,Cytokines ,Medicine ,lcsh:Q ,medicine.symptom ,Research Article - Abstract
Interleukin 22 (IL-22) is a T-cell secreted cytokine that modulates inflammatory response in nonhematopoietic tissues such as epithelium and liver. The function of IL-22 in adipose tissue is currently unknown. We generated a transgenic mouse model with overexpression of IL-22 specifically in adipose tissue. The IL-22 transgenic mice had no apparent changes in obesity and insulin resistance after feeding with high fat diet (HFD). Unexpectedly, all the IL-22 transgenic mice fed with HFD for four months developed spontaneous tumors in epididymal adipose tissue. Histological analysis indicated that the tumors were well-differentiated liposarcomas with infiltration of inflammatory cells. IL-22 overexpression promotes production of inflammatory cytokines such as IL-1β and IL-10 and stimulates ERK phosphorylation in adipose tissue. Furthermore, IL-22 treatment in differentiated 3T3-L1 adipocytes could induce IL-1β and IL-10 expression, together with stimulation of ERK phosphorylation. Taken together, our study not only established a novel mouse model with spontaneous liposarcoma, but also revealed that IL-22 overexpression may collaborate with diet-induced obesity to impact on tumor development in mouse.
- Published
- 2011
11. Mitosis Phase Enrichment with Identification of Mitotic Centromere-Associated Kinesin As a Therapeutic Target in Castration-Resistant Prostate Cancer.
- Author
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Sircar, Kanishka, Heng Huang, Limei Hu, Yuexin Liu, Dhillon, Jasreman, Cogdell, David, Aprikian, Armen, Efstathiou, Eleni, Navone, Nora, Troncoso, Patricia, and Wei Zhang
- Subjects
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PROSTATE cancer , *MITOSIS , *DRUG therapy , *CASTRATION , *GENE expression , *PROTEINS - Abstract
The recently described transcriptomic switch to a mitosis program in castration-resistant prostate cancer (CRPC) suggests that mitotic proteins may be rationally targeted at this lethal stage of the disease. In this study, we showed upregulation of the mitosis-phase at the protein level in our cohort of 51 clinical CRPC cases and found centrosomal aberrations to also occur preferentially in CRPC compared with untreated, high Gleason-grade hormone-sensitive prostate cancer (P<0.0001). Expression profiling of chemotherapy-resistant CRPC samples (n = 25) was performed, and the results were compared with data from primary chemotherapy-naïve CRPC (n = 10) and hormone-sensitive prostate cancer cases (n = 108). Our results showed enrichment of mitosis-phase genes and pathways, with progression to both castration-resistant and chemotherapyresistant disease. The mitotic centromere-associated kinesin (MCAK) was identified as a novel mitosis-phase target in prostate cancer that was overexpressed in multiple CRPC gene-expression datasets. We found concordant gene expression of MCAK between our parent and murine CRPC xenograft pairs and increased MCAK protein expression with clinical progression of prostate cancer to a castration-resistant disease stage. Knockdown of MCAK arrested the growth of prostate cancer cells suggesting its utility as a potential therapeutic target. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
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