63 results on '"Dian‐Peng Li"'
Search Results
2. Lindenane‐Type Sesquiterpene Dimers Mitigate Lipopolysaccharide‐Induced Inflammation by Inhibiting Toll‐Like Receptor Signaling
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Xue‐Rong Yang, Jing‐Ru Song, Xiao‐Hua Jiang, Fei Dong, Xiao‐Jie Yan, Jian‐Xing Li, Xue‐Ying Zhao, Dian‐Peng Li, and Feng‐Lai Lu
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Molecular Medicine ,Bioengineering ,General Chemistry ,General Medicine ,Molecular Biology ,Biochemistry - Published
- 2023
3. Design, synthesis, and antiproliferative evaluation of novel longifolene-derived tetraline pyrimidine derivatives with fluorescence properties
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Qing-Min Li, Gui-Shan Lin, Wen-Gui Duan, Yu-Cheng Cui, Fang-Yao Li, Fu-Hou Lei, and Dian-Peng Li
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Materials Chemistry ,General Chemistry ,Catalysis - Abstract
In the search for novel compounds with both survivin inhibitory activity and fluorescence properties, 18 novel longifolene-derived tetralin pyrimidine compounds were designed using survivin as the target and synthesized from the sustainable natural resource longifolene.
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- 2022
4. Rhodomollosides A and B, glycosides of methyl everninate from the aerial parts of Rhododendron molle
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Xue-Rong Yang, Naonobu Tanaka, Jing-Ru Song, Feng-Lai Lu, Xiao-Jie Yan, Jian-Xing Li, Xue-Ying Zhao, Yoshiki Kashiwada, and Dian-Peng Li
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Pharmacology ,Complementary and alternative medicine ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,General Medicine ,Analytical Chemistry - Published
- 2021
5. Acylated iridoid glucoside and xanthones from Canscora lucidissima: Their structures and ferroptosis inhibitory activity
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Naonobu Tanaka, Ikko Shibata, Yusuke Tasaki, Yuki Yoshino, Daisuke Tsuji, Feng-Lai Lu, Xiao-Jie Yan, Xue-Rong Yang, Kohji Itoh, Reiko Akagi, Dian-Peng Li, and Yoshiki Kashiwada
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Pharmacology ,Drug Discovery ,General Medicine - Published
- 2023
6. Design and synthesis of mogrol derivatives modified on a ring with anti-inflammatory and anti-proliferative activities
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Jing-Ru Song, Na Li, Yu-Lu Wei, Feng-Lai Lu, and Dian-Peng Li
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History ,Lung Neoplasms ,Polymers and Plastics ,Organic Chemistry ,Clinical Biochemistry ,Anti-Inflammatory Agents ,NF-kappa B ,Pharmaceutical Science ,Antineoplastic Agents ,Biochemistry ,Industrial and Manufacturing Engineering ,Carcinoma, Non-Small-Cell Lung ,Drug Discovery ,Molecular Medicine ,Humans ,Business and International Management ,Molecular Biology - Abstract
A class of novel mogrol derivatives modified on A ring were synthesized. The screening result showed that indole-fused derivatives exhibited lower toxicity and better anti-inflammatory activity in LPS-induced RAW 264.7 cells model than mogrol and other compounds. Derivative B8 exerted superior inhibitory result of NO production (IC
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- 2022
7. Enhanced hydrogen generation performances and mechanism of Al-water reaction catalyzed by flower-like BiOCl@CNTs
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Tianhao Zhou, Fen Xu, Lixian Sun, Jiaxi Liu, Xiaolei Guo, Sheng Wei, Chenchen Zhang, Lumin Liao, Jinfan Wu, Zirong Huang, Kexiang Zhang, and Dian-Peng Li
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Biomaterials ,Materials Chemistry ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2023
8. [Effects of simulated rainfall enhancement on sediment CO
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Yu-Xin, Zhang, Jing-Bai-Lun, Jiang, Dian-Peng, Li, Mei-Si, Yao, Tao, Sun, Jian-Qin, Zhou, and Hong-Tao, Jia
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China ,Greenhouse Gases ,Lakes ,Seasons ,Carbon Dioxide ,Methane - Abstract
Understanding the responses of lake sediment carbon process to climate change is an important part of a comprehensive understanding of lake carbon budget. To explore the effects of future rainfall increase on sediment carbon flux, undisturbed sediment samples were collected from the bottom of dry lake Barikun in Hami, Xinjiang for the incubation experiment. Based on the increase rate of precipitation (4 mm·10 a明确湖泊沉积物碳过程对气候变化的响应,是全面了解湖泊生态系统碳收支的重要环节。为探究未来降雨增加对沉积物碳通量的影响,本研究以新疆哈密巴里坤盐湖干涸湖底原状沉积物为对象,基于1960年以来新疆哈密地区降雨量增加速率(4 mm·10 a
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- 2022
9. Comparative Study on the Antidiabetic Efficacy of Unripe and Ripe Fruit Extracts of Siraitia grosvenorii and the Possible Mechanism of Action
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Yang Ziming, Xiaojie Yan, Xiangli Xu, Theophine Chinwuba Akunne, Dian-Peng Li, and Fenglai Lu
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Mechanism of action ,Traditional medicine ,Fruit extracts ,medicine ,food and beverages ,medicine.symptom ,Biology ,Siraitia grosvenorii - Abstract
Aims: The ripe fruit of Siraitia grosvenorii (Swingle) C. Jeffrey (Cucurbitaceae) is used in Traditional Chinese Medicine (TCM) for treatment of diabetes. The ripe fruit is very edible, nutritious and of high sweet taste, while the unripe fruit which is usually very bitter and not edible, has not been widely studied. Therefore, the aim of this study is to evaluate the antidiabetic activity of the unripe fruit of S. grosvenorii and to compare it with that of the ripe fruit as well as to determine their possible mechanism of action. Methodology: The method of streptozotocin-nicotinamide induced diabetic animal model in mice was employed and mice with blood glucose greater than 9.0 mmol/l were selected for treatment with unripe fruit extract (UFE) and ripe fruit extract (RFE) for 24 days. Alpha-glucosidase and alpha amylase enzymes inhibitory assays for determination of possible mechanism of action, were performed. Results: The RFE exhibited better efficacy in antidiabetic activity than the UFE, though the later displayed better potency in activity. The RFE, UFE (300 mg/kg) and glibenclamide exhibited significant (P
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- 2020
10. Preparative Isolation of Piceatannol Derivatives from Passion Fruit (Passiflora edulis) Seeds by High-Speed Countercurrent Chromatography Combined with High-Performance Liquid Chromatography and Screening for α-Glucosidase Inhibitory Activities
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Zhi-Qi Zou, Ling-Ling Yu, Yun-Chang Xie, Li Lianchun, Zheng-Hong Pan, De-Sheng Ning, Fu Yuxia, and Dian-Peng Li
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0106 biological sciences ,Piceatannol ,Circular dichroism ,Chromatography ,biology ,Electrospray ionization ,010401 analytical chemistry ,General Chemistry ,biology.organism_classification ,01 natural sciences ,High-performance liquid chromatography ,0104 chemical sciences ,Solvent ,Passiflora ,chemistry.chemical_compound ,Countercurrent chromatography ,chemistry ,Polyphenol ,General Agricultural and Biological Sciences ,010606 plant biology & botany - Abstract
Passiflora edulis Sims (passion fruit) seeds are often discarded as byproducts during juice processing. In fact, the seeds are of considerable commercial value in the food and cosmetics industry because of their rich polyphenols, especially piceatannol. In this study, high-speed countercurrent chromatography (HSCCC) was applied for the separation of stilbene polyphenols from passion fruit seeds. The n-hexane-ethyl acetate-methanol-water (1:2:1:2.8, v/v) was found to be the optimum two-phase solvent for the preparation of two major stilbenes, scirpusin B (8) and piceatannol (9) with purities of 90.2% and 94.8%, respectively. In addition, a continuous semipreparative HPLC was applied to further purify the HSCCC fractions containing minor stilbenes and obtain four new piceatannol derivatives (1-4) along with three known ones (5-7). The structures of these new compounds were determined using spectroscopic methods, including NMR, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), and circular dichroism (CD). The isolated compounds were evaluated for α-glucosidase inhibitory activities in vitro. The result suggested that all of them exhibited more significant activity than acarbose, and passiflorinol B (2) had the strongest activity, with a IC50 value of 1.7 μM.
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- 2020
11. Structural Characterization and Assessment of Anti-Inflammatory Activities of Polyphenols and Depsidone Derivatives from
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Rui-Jie, He, Ya-Feng, Wang, Bing-Yuan, Yang, Zhang-Bin, Liu, Dian-Peng, Li, Bi-Qun, Zou, and Yong-Lin, Huang
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Lactones ,Plant Extracts ,Melastomataceae ,Anti-Inflammatory Agents ,Polyphenols ,Depsides - Abstract
The roots of
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- 2022
12. Two new isoquinolines from
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Zhi-Wei, Ouyang, Ji-Man, He, Feng, Qin, Ling, Li, Wen-Di, Tang, Dian-Peng, Li, and Jiang-Ke, Qin
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Two new isoquinolines (
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- 2022
13. The generally useful estimate of solvent systems method facilitates off‐line two‐dimensional countercurrent chromatography for isolating compositions from Siraitia grosvenorii roots
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Fenglai Lu, Jiayi Sun, Xiaohua Jiang, Xuerong Yang, Hongwei Liu, Xiaojie Yan, Yueyuan Chen, and Dian‐Peng Li
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Filtration and Separation ,Analytical Chemistry - Abstract
Solvent system selection is a crucial and the most time-consuming step for successful countercurrent chromatography separation. A thin-layer chromatography based generally useful estimate of solvent systems method has been developed to simplify the solvent system selection. We herein utilized the method to select a solvent system for off-line two-dimensional high-performance countercurrent chromatography to separate chemical compositions from a complex fraction of the Siraitia grosvenorii root extract. The first-dimensional countercurrent separation using chloroform/methanol/water (10:5.5:4.5, v/v) yielded four compounds with high purity and three mixture fractions (Fr I, III, and VII). The second-dimensional countercurrent separation conducted on Fr I, III, and VII using the hexane/ethyl acetate/methanol/water (4:6:6:4, 3:7:3:7, v/v) and chloroform/methanol/water (10:9:6, v/v) solvent systems, respectively, produced another four compounds. Four triterpenoids and four lignans were finally isolated, including two novel compounds. Hence, the generally useful estimate of solvent systems method is a feasible and efficient approach for selecting an applicable solvent system for separating complex samples. In addition, the off-line two-dimensional countercurrent chromatography method can improve both the peak resolution and the capacity of countercurrent chromatography. This article is protected by copyright. All rights reserved.
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- 2023
14. Vicenin 3 ameliorates ECM degradation by regulating the MAPK pathway in SW1353 chondrocytes
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Xiao-Hua Jiang, Yueyuan Chen, Xue-Rong Yang, Dian-Peng Li, Xiao-Jie Yan, and Fenglai Lu
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MAPK/ERK pathway ,Cancer Research ,Thrombospondin ,Metalloproteinase ,Chemistry ,p38 mitogen-activated protein kinases ,Articles ,General Medicine ,Matrix metalloproteinase ,Chondrocyte ,Cell biology ,Extracellular matrix ,osteoarthritis ,medicine.anatomical_structure ,vicenin 3 ,Immunology and Microbiology (miscellaneous) ,MMP-13 ,MAPK signaling pathway ,medicine ,collagen type II and aggrecan ,Aggrecan - Abstract
Aberrant destruction of the articular extracellular matrix (ECM) has been considered to be one of the pathological features of osteoarthritis (OA) which results in chondrocyte changes and articular cartilage degeneration. The MAPK signaling pathway serves a key role by releasing cartilage-degrading enzymes from OA chondrocytes. However, the use of MAPK inhibitors for OA is hindered by their potential long-term toxicity. Vicenin 3 is one of the major components of the Jian-Gu injection which is effective in the clinical treatment of OA. However, its potential impact on OA remain poorly understood. Therefore, the present study aimed to assess the effects of vicenin 3 on interleukin (IL)-1β-treated SW1353 chondrocytes, which mimic the microenvironment of OA. These chondrocytes were pretreated with vicenin 3 (0, 5 and 20 µM) for 1 h and subsequently stimulated with IL-1β (10 ng/ml) for 24 h. Nitric oxide (NO) production was measured using the Griess reaction, whereas the production of prostaglandin E2 (PGE2), matrix metalloproteinases (MMPs), A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTSs), collagen type II and aggrecan were measured using ELISA. The mRNA expression of MMPs and ADAMTSs were measured using reverse transcription-quantitative PCR. The protein expression levels of MAPK were measured using western blotting. Vicenin 3 was found to significantly inhibit IL-1β-induced production of NO and PGE. Increments in the expression levels of MMP-1, MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5 induced by IL-1β, in addition to the IL-1β-induced degradation of collagen type II and aggrecan, were all reversed by vicenin 3 treatment. Furthermore, vicenin 3 suppressed IL-1β-stimulated MAPK activation, an effect that was similar to that exerted by SB203580, a well-known p38 MAPK inhibitor. In conclusion, vicenin 3 may confer therapeutic potential similar to that of the p38 MAPK inhibitor for the treatment of OA.
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- 2021
15. Three new compounds from the leaves of
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Ya-Feng, Wang, Rui-Jie, He, Dian-Peng, Li, and Yong-Lin, Huang
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Plant Leaves ,Monophenol Monooxygenase ,Plant Extracts ,Methanol ,Glycoside Hydrolase Inhibitors ,alpha-Glucosidases ,Lipase ,Fagaceae - Abstract
Three new compounds (6
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- 2021
16. Three new compounds from the leaves of Castanopsis tibetana Hance
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Yong-Lin Huang, Dian-Peng Li, Rui-Jie He, and Ya-Feng Wang
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chemistry.chemical_compound ,chemistry ,Castanopsis tibetana ,Organic Chemistry ,Plant Science ,Purpurogallin ,Carboxylate ,Biochemistry ,Analytical Chemistry ,Nuclear chemistry - Abstract
Three new compounds (6S,9S)-6′-galloyl-roseoside (1), purpurogallin ethyl carboxylate (2), and tibetana A (3) were isolated from 80% methanol extract of the leaves of Castanopsis tibetana Hance. Their structures were elucidated based on comprehensive spectroscopic methods and chemical data, including optical rotation, UV, MS, 1 D and 2 D NMR spectra. Compounds 1 and 3 were evaluated for their α-glucosidase inhibitory activity, pancreatic lipase inhibitory activity, and tyrosinase inhibitory activity.
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- 2021
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17. Structural Characterization and Assessment of Anti-Inflammatory Activities of Polyphenols and Depsidone Derivatives from Melastoma malabathricum subsp. normale
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Rui-Jie He, Ya-Feng Wang, Bing-Yuan Yang, Zhang-Bin Liu, Dian-Peng Li, Bi-Qun Zou, and Yong-Lin Huang
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Chemistry (miscellaneous) ,Organic Chemistry ,Drug Discovery ,Melastoma malabathricum subsp. normale (D. Don) Karst. Mey ,Melastoma ,polyphenols ,complex tannin ,depsidone derivatives ,anti-inflammatory ,Molecular Medicine ,Pharmaceutical Science ,Physical and Theoretical Chemistry ,Analytical Chemistry - Abstract
The roots of Melastoma malabathricum subsp. normale (D. Don) Karst. Mey have been used in traditional ethnic medicine systems in China to treat inflammation-triggered ailments, such as trauma, toothache, and fever. Therefore, the aim of this study is to screen for compounds with anti-inflammatory activity in the title plant. The extract of M. malabathricum subsp. normale roots was separated using various chromatographic methods, such as silica gel, ODS C18, MCI gel, and Sephadex LH-20 column chromatography, as well as semi-preparative HPLC. One new complex tannin, named whiskey tannin D (1), and an undescribed tetracyclic depsidone derivative, named guanxidone B (2), along with nine known polyphenols (2–10) and three known depsidone derivatives (12–14) were obtained from this plant. The structures of all compounds were elucidated by extensive NMR and CD experiments in conjunction with HR-ESI-MS data. All these compounds were isolated from this plant for the first time. Moreover, compounds 1–4, 8, and 10–14 were obtained for the first time from the genus Melastoma, and compounds 1, 2, and 11–14 have not been reported from the family Melastomataceae. This is the first report of complex tannin and depsidone derivatives from M. malabathricum subsp. normale, indicating their chemotaxonomic significance to this plant. Compounds 1–12 were investigated for their anti-inflammatory activities on the production of the nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and compounds 1, 11, and 12 showed anti-inflammatory activities with IC50 values of 6.46 ± 0.23 µM, 8.02 ± 0.35 µM, and 9.82 ± 0.43 µM, respectively. The structure–activity relationship showed that the catechin at glucose C-1 in ellagitannin was the key to its anti-inflammatory activity, while CH3O- at C-16 of aromatic ring A in depsidone derivatives had little effect on its anti-inflammatory activity. The study of structure–activity relationships is helpful to quickly discover new anti-inflammatory drugs. The successful isolation and structure identification of these compounds, especially complex tannin 1, not only provide materials for the screening of anti-inflammatory compounds, but also provide a basis for the study of chemical taxonomy of the genus Melastoma.
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- 2022
18. Classification of Flavonoid Metabolomes via Data Mining and Quantification of Hydroxyl NMR Signals
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Dian-Peng Li, Richard B. van Breemen, Shao-Nong Chen, Lingyi Huang, Yang Yu, David C. Lankin, Li-She Gan, Guido F. Pauli, and James B. McAlpine
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chemistry.chemical_classification ,Flavonoids ,Chromatography ,Magnetic Resonance Spectroscopy ,Molecular Structure ,Extramural ,010401 analytical chemistry ,Flavonoid ,Nuclear magnetic resonance spectroscopy ,010402 general chemistry ,01 natural sciences ,Article ,0104 chemical sciences ,Analytical Chemistry ,chemistry ,Hydroxides ,Data Mining - Abstract
Utilizing the distinct HMBC crossed-peak patterns of lower-field range (LFR; 11.80‒14.20 ppm) hydroxyl singlets, presented NMR methodology characterizes flavonoid metabolomes both qualitatively and quantitatively. It enables simultaneous classification of the structural types of 5-OH flavonoids and biogenetically related 2’-OH chalcones, as well as quantification of individual metabolites from (1)H NMR spectra, even in complex mixtures. Initially, metabolite-specific LFR 1D (1)H and 2D HMBC patterns were established via literature mining and experimental data interpretation, demonstrating that LFR HMBC patterns encode the different structural types of 5-OH flavonoids/2’-OH chalcones. Taking advantage of the simplistic multiplicity of the H,H-uncoupled LFR 5-/2’-OH singlets, individual metabolites could subsequently be quantified by peak fitting quantitative (1)H NMR (PF-qHNMR). Metabolomic analysis of enriched fractions from three medicinal licorice (Glycyrrhiza) species established proof-of-concept for distinguishing three major structural types and eight subtypes in biomedical applications. The method identified fifteen G. uralensis (GU) phenols from the six possible subtypes of 5,7-diOH (iso)flav(an)ones with 6-, 8-, and non-prenyl substitution, including the new 6-prenyl-licoisoflavanone (1) and two previously unknown compounds (4 and 7). Relative (100%) qNMR established quantitative metabolome patterns suitable for species discrimination and plant metabolite studies. Absolute qNMR with combined external and internal (solvent) calibration (ECIC) identified and quantified 158 GU metabolites. HMBC-supported qHNMR analysis of flavonoid metabolomes (“flavonomics”) empowers the exploration of structure-abundance-activity relationships of designated bioactivity. Its ability to identify and quantify numerous metabolites simultaneously and without identical reference materials opens new avenues for natural product discovery and botanical quality control and can be adopted to other flavonoid- and chalcone containing taxa.
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- 2020
19. Preparative Isolation of Piceatannol Derivatives from Passion Fruit (
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Zheng-Hong, Pan, De-Sheng, Ning, Yu-Xia, Fu, Dian-Peng, Li, Zhi-Qi, Zou, Yun-Chang, Xie, Ling-Ling, Yu, and Lian-Chun, Li
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Passiflora ,Plant Extracts ,Fruit ,Seeds ,Stilbenes ,Glycoside Hydrolase Inhibitors ,alpha-Glucosidases ,Countercurrent Distribution ,Chromatography, High Pressure Liquid - Published
- 2020
20. Synthesis and anti-proliferation activity of mogrol derivatives bearing quinoline and triazole moieties
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Na Li, Jing-Ru Song, and Dian-Peng Li
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Clinical Biochemistry ,Pharmaceutical Science ,Antineoplastic Agents ,Apoptosis ,01 natural sciences ,Biochemistry ,Structure-Activity Relationship ,chemistry.chemical_compound ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,Cytotoxicity ,STAT3 ,Molecular Biology ,Cell Proliferation ,A549 cell ,Dose-Response Relationship, Drug ,Molecular Structure ,biology ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,Quinoline ,Cancer ,Cell Cycle Checkpoints ,Triazoles ,Cell cycle ,medicine.disease ,In vitro ,respiratory tract diseases ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Quinolines ,biology.protein ,Molecular Medicine ,Drug Screening Assays, Antitumor - Abstract
A series of novel derivatives based on mogrol were designed and synthesized in attempt to improve anti-lung cancer activity. The cytotoxicity against human lung cancer cells including A549 and NCI-H460 were performed by Cell Counting Kit-8 (CCK8) assay in vitro. The screening result showed that compound 8f exhibited the strongest activity with an IC50 value of 4.47 μM against A549 cell, and could induce the cell apoptosis in a dose-dependent manner and arrest cell cycle at G0/G1 phase. Besides, compound 8f displayed anti-proliferation effect on A549 cell through inhibiting phosphorylation of signal transducer and activator of transcription 3 (STAT3). Furthermore, compared with morgol, compound 10a significantly improved the cytotoxicity against NCI-H460 with the IC50 value of 17.13 μM. The research stimulated the development of potential therapeutic agent for lung cancer from the natural mogrol.
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- 2021
21. Soil microorganisms alleviate the allelopathic effect of Eucalyptus grandis × E. urophylla leachates on Brassica chinensis
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Fenglai Lu, Huashou Li, Dian-Peng Li, Yueyuan Chen, Rensen Zeng, and Lilang Zheng
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0106 biological sciences ,biology ,Microorganism ,fungi ,Brassica ,food and beverages ,Biomass ,Forestry ,Plant community ,biology.organism_classification ,complex mixtures ,010603 evolutionary biology ,01 natural sciences ,Eucalyptus ,Horticulture ,Germination ,Soil water ,Botany ,Allelopathy ,010606 plant biology & botany - Abstract
Soil microbes may be critical players in determining the allelopathic potential of some plants. Low levels of plant community biodiversity in Eucalyptus plantations have been attributed to the allelopathic potential of these tree species. The role of soil microbes in the allelopathic effect of leaf leachates of the hybrid tree Eucalyptus grandis × E. urophylla, was tested in Petri dish assays with Brassica chinensis as a receiver plant. Soils were collected from either a local garden (soil A) or a Eucalyptus plantation (soil B) and half of each soil was sterilized to remove microbes. These soils were then treated with E. grandis × E. urophylla leachates for 0–72 h. Seed germination of B. chinensis was significantly inhibited in soils treated with leaf leachates relative to untreated soils. The inhibitory effect of the leaf leachates was more pronounced in sterilized soils. Total phenolic content was obvious lower in nonsterile leachate-treated soils than in sterile soils. Biomass of B. chinensis was negatively correlated with the total phenolic content in soils. Our findings suggest that soil microbes can alleviate the allelopathic potential of Eucalyptus and thereby its negative impact on plant growth.
- Published
- 2017
22. Phytochemical and chemotaxonomic study on Castanopsis fargesii Franch
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Dian-Peng Li, Ya-Feng Wang, Yong-Lin Huang, and Rui-Jie He
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chemistry.chemical_classification ,food.ingredient ,biology ,Traditional medicine ,010405 organic chemistry ,Plant composition ,Castanopsis ,010402 general chemistry ,biology.organism_classification ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Fagaceae ,food ,Triterpene ,chemistry ,Phytochemical ,Chemotaxonomy ,Genus ,Ecology, Evolution, Behavior and Systematics ,Castanopsis fargesii - Abstract
In the course of a phytochemical and chemotaxonomical investigation for the Castanopsis (Fagaceae) species, fifteen compounds, including two triterpene hexahydroxydiphenoyl esters (1 and 2), seven gallotannins (3–9), three flavonoids (10–12), and three other compounds (13–15) were isolated from the leaves of Castanopsis fargesii Franch. Their structures were elucidated by means of spectroscopic methods and in comparison with published data. All these compounds were obtained from this species for the first time. Moreover, ethyl glucopyranoside (15) was firstly isolated from the genus Castanopsis. 6-O-Galloyl-β- d -glucose (3) and 3,4′,5,7-tetrahydroxyflavone (11) have not been reported from any species in Fagaceae family. The chemotaxonomic significance of these compounds was summarized.
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- 2018
23. Hypoglycemic effects of esculeoside A are mediated via activation of AMPK and upregulation of IRS-1
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Yueyuan Chen, Jinglei Liu, Fenglai Lu, Lei Wang, Dian-Peng Li, Yang Ziming, and Zhang Li
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Male ,AMPK ,medicine.medical_specialty ,Sapogenins ,medicine.medical_treatment ,AMP-Activated Protein Kinases ,db/db mice ,Liver glucose metabolism ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Downregulation and upregulation ,Internal medicine ,Diabetes mellitus ,Glucokinase ,medicine ,Animals ,Humans ,Hypoglycemic Agents ,Insulin ,Phosphorylation ,IRS-1 ,Protein kinase A ,Esculeoside a ,biology ,Chemistry ,lcsh:Other systems of medicine ,General Medicine ,lcsh:RZ201-999 ,medicine.disease ,Up-Regulation ,030205 complementary & alternative medicine ,Mice, Inbred C57BL ,Insulin receptor ,Endocrinology ,Diabetes Mellitus, Type 2 ,Complementary and alternative medicine ,030220 oncology & carcinogenesis ,Insulin Receptor Substrate Proteins ,biology.protein ,Phosphoenolpyruvate carboxykinase ,Research Article - Abstract
Background Tomato fruit (Lycopersicon esculentum Mill.) has been suggested to be useful for the prevention of diabetes. Esculeoside A is the main saponin compounds in tomatoes. This study investigated the hypoglycemic effects and the underlying mechanism of esculeoside A in C57BLKS/Leprdb (db/db) mice. Methods Wild-type C57BLKS (db/dm) mice were used in the db/dm mouse group and db/db mice were randomly divided into 2 groups: untreated and treated db/db mouse groups. Esculeoside A (100 mg/kg) was administered by gavage for 56 days to the treated db/db mouse group. Distilled water was administered to the db/dm mouse group and the untreated db/db mouse group. The blood and liver biochemical parameters and the expression of liver insulin signaling-related proteins were examined. Results The results showed that esculeoside A reduced the fasting blood glucose (FBG) levels and improved the glucose tolerance. Further investigation revealed that hepatic protein expressions of total AMP-activated protein kinase (T-AMPK), phosphorylated AMP-activated protein kinase (p-AMPK), insulin receptor substrate-1 (IRS-1), and glucokinase (GCK) were significantly upregulated after esculeoside A treatment. In contrast, the hepatic protein expression of phosphoenolpyruvate carboxykinase (PEPCK) was significantly downregulated by esculeoside A treatment. Conclusion These findings suggested that esculeoside A has a potential of alleviating the metabolic abnormalities in db/db mice via regulation of AMPK/IRS-1 pathway. Our findings supported a possible application of esculeoside A as a functional supplement for diabetes treatment.
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- 2019
24. Ellagitannins and Oligomeric Proanthocyanidins of Three Polygonaceous Plants
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Takashi Tanaka, Yun-Qiu Li, Ya-Feng Wang, Zhi-Hong Jiang, Yosuke Matsuo, Yoshinori Saito, Juri Takayoshi, Dian-Peng Li, Gen-Ichiro Nonaka, Yong-Lin Huang, and Masako Kitaoka
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Models, Molecular ,Polygonum ,Magnetic Resonance Spectroscopy ,Hydrolyzable Tannin ,Pharmaceutical Science ,Persicaria ,ellagitannin ,010402 general chemistry ,01 natural sciences ,Article ,Polygonaceae ,Analytical Chemistry ,lcsh:QD241-441 ,chemistry.chemical_compound ,lcsh:Organic chemistry ,Ellagitannin ,hydrolyzable tannin ,Drug Discovery ,Organic chemistry ,Proanthocyanidins ,Physical and Theoretical Chemistry ,proanthocyanidin ,chemistry.chemical_classification ,biology ,010405 organic chemistry ,Organic Chemistry ,Polyphenols ,biology.organism_classification ,Hydrolyzable Tannins ,0104 chemical sciences ,Rhizome ,chemistry ,Proanthocyanidin ,Chemistry (miscellaneous) ,Molecular Medicine ,Ellagic acid - Abstract
The aim of this study was to characterize hydrolyzable tannins in Polygonaceous plants, as only a few plants have previously been reported to contain ellagitannins. From Persicaria chinensis, a new hydrolyzable tannin called persicarianin was isolated and characterized to be 3-O-galloyl-4,6-(S)-dehydrohexahydroxydiphenoyl-d-glucose. Interestingly, acid hydrolysis of this compound afforded ellagic acid, despite the absence of a hexahydroxydiphenoyl group. From the rhizome of Polygonum runcinatum var. sinense, a large amount of granatin A, along with minor ellagitannins, helioscpoinin A, davicratinic acids B and C, and a new ellagitannin called polygonanin A, were isolated. Based on 2D nuclear magnetic resonance (NMR) spectroscopic examination, the structure of polygonanin A was determined to be 1,6-(S)-hexahydroxydiphenoyl-2,4-hydroxychebuloyl-β-d-glucopyranose. These are the second and third hydrolyzable tannins isolated from Polygonaceous plants. In addition, oligomeric proanthocyanidins of Persicaria capitatum and P. chinensis were characterized by thiol degradation. These results suggested that some Polygonaceous plants are the source of hydrolyzable tannins not only proanthocyanidins., Molecules, 26(2), art.no.337; 2021
- Published
- 2021
25. Sarcaglabrin A, a conjugate of C15 and C10 terpenes from the aerial parts of Sarcandra glabra
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Naonobu Tanaka, Daisuke Tsuji, Xue-Rong Yang, Dian-Peng Li, Yoshiki Kashiwada, Fenglai Lu, Xiao-Jie Yan, and Kohji Itoh
- Subjects
Ocimene ,Terpene ,chemistry.chemical_compound ,chemistry ,Stereochemistry ,Monoterpene ,Organic Chemistry ,Drug Discovery ,Sesquiterpene ,Biochemistry ,Human cancer ,Sarcandra glabra ,Conjugate - Abstract
A new C25 terpene consisting of C15 and C10 terpenes, sarcaglabrin A, and two new sesquiterpene dimers, sarcaglabrins B and C, were isolated from the aerial parts of a medicinal plant Sarcandra glabra used in Guangxi Zhuang Autonomous Region, China. Their structures were elucidated on the basis of detailed spectroscopic analyses including calculation of the ECD spectrum. Sarcaglabrin A was assigned as a conjugate of lindenane type sesquiterpene and ocimene type monoterpene, while sarcaglabrins B and C were elucidated to be lindenane sesquiterpene dimers. Their cytotoxicity against human cancer cell lines were evaluated.
- Published
- 2020
26. Limonoids from the aerial parts of Munronia pinnata
- Author
-
Kohji Itoh, Xiao-Jie Yan, Dian-Peng Li, Yoshiki Kashiwada, Daisuke Tsuji, Xue-Rong Yang, Fenglai Lu, and Naonobu Tanaka
- Subjects
Meliaceae ,biology ,010405 organic chemistry ,Chemistry ,Stereochemistry ,Organic Chemistry ,Prieurianin ,010402 general chemistry ,biology.organism_classification ,Limonoid ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Munronia pinnata ,Drug Discovery ,medicine ,Moiety ,medicine.drug - Abstract
Six new limonoids, munropins A−F (1–6), were isolated from the aerial parts of a Chinese medicinal plant, Munronia pinnata (Meliaceae). The structures of 1–6 were assigned by detailed analyses of their spectroscopic data. Munropins A (1) and B (2) are limonoids possessing a prieurianin skeleton with α,β-unsaturated γ-lactam moieties at C-17. Munropins C (3), D (4), and E (5) are also prieurianin type limonoids with an α,β-unsaturated γ-lactone moiety, an acetyl group, and an acetoxyacetyl group at C-17, respectively. Munropin F (6) was assigned as a nimbolinin type limonoid with a γ-hydroxy-α,β-unsaturated γ-lactone moiety.
- Published
- 2019
27. [Study on the Chemical Constituents of Litchi chinensis Pericarp( Ⅱ)]
- Author
-
Xiao-li, Guan, Yong-lin, Huang, Ya-feng, Wang, and Dian-peng, Li
- Subjects
Glucosides ,Litchi ,Proanthocyanidins ,Quercetin ,Glycosides ,Catechin ,Chromatography, High Pressure Liquid ,Mass Spectrometry ,Drugs, Chinese Herbal - Abstract
To study the chemical constituents of Litchi chinensis pericarp.The compounds were isolated by Sephadex LH-20, MCI gel CHP 20 P, Toyopearl Butly-650 C, Toyopearl WH-40 F column chromatographies and semi-preparative HPLC, the structures were elucidated by NMR and mass spectroscopic MS data analysis.14 compounds were obtained and their structures were identified as quercetin( 1), chrysoeriol( 2),kaemperol-3-O-β-D-glucoside( 3), manghaslin( 4), isorhamnetin-3-O-robinobioside( 5), ( +)-gallocatechin( 6), (-) epicatechin-3-O-gallate( 7), cinnamtannin B-1( 8), isolariciresinol-9-O-β-D-xyloside( 9), ( +)-5-methoxyisolariciresinol-9-O-β-D-xylopyranoside( 10), vanillic acid( 11), 3, 4, 3’, 4’-tetrahydroxy biphenyl( 12), tachioside( 13) and isotachioside( 14).Compounds 1 ~ 7,9 ~ 14 are obtained from this plant pericarp for the first time.
- Published
- 2018
28. New clerodane diterpenoids from the twigs and leaves of Croton euryphyllus
- Author
-
Xing-De Wu, Dian-Peng Li, De-Sheng Ning, Shihong Lv, Zheng-Hong Pan, and Huang Sisi
- Subjects
Magnetic Resonance Spectroscopy ,Stereochemistry ,Dimer ,Clinical Biochemistry ,Molecular Conformation ,Pharmaceutical Science ,Crystallography, X-Ray ,Ring (chemistry) ,PC12 Cells ,Biochemistry ,Diterpenes, Clerodane ,Cyclobutane ,chemistry.chemical_compound ,Nerve Growth Factor ,Drug Discovery ,Neurites ,Animals ,Molecular Biology ,Cell Proliferation ,Organic Chemistry ,Croton euryphyllus ,Cycloaddition ,Terpenoid ,Rats ,Plant Leaves ,chemistry ,Molecular Medicine ,Croton - Abstract
Three new nor-clerodane diterpenoids, crotoeurins A-C (1-3), together with four known ones were isolated from the twigs and leaves of Croton euryphyllus. Among them, crotoeurin A (1) is a new nor-clerodane diterpenoid dimer with a unique cyclobutane ring via a [2+2] cycloaddition. Their structures were elucidated by spectroscopic analyses and the stereochemistry of 1 was confirmed by single-crystal X-ray diffraction analysis. Compounds 1-3 exhibited neurite outgrowth-promoting activity on NGF-mediated PC12 cells at concentration of 10 mu M. (C) 2015 Elsevier Ltd. All rights reserved.
- Published
- 2015
29. Chemical Characterization of the Essential Oils of Eucalyptus.grandis×Eucalyptus.urophylla Hybrids and Six Pure Eucalyptus species Grown in Guangxi (China)
- Author
-
Dian Peng Li, Jiao Hong Wei, Yong-Lin Huang, Zhen Yi Liu, Feng Lai Lu, and Yue Yuan Chen
- Subjects
Horticulture ,Chemotype ,General Engineering ,Composition (visual arts) ,Gas chromatography–mass spectrometry ,Biology ,Pulp and paper industry ,Chemical composition ,Eucalyptus ,Hybrid - Abstract
Hydrodistillation of the fresh leaves of E.grandis×E.urophylla hybrids and pure species E.grandis, E.urophylla, E.pellita, E.camaldulensis, E. dunni, and E.saligna collected from the trees grown in Guangxi Huangmian Forest (China), afforded essential oils in yields varying from 0.24±0.15% to 5.36±0.28%, according to the species. Analysis by GC (FID) and GC/MS allowed the identification of 67 compounds, representing 82.55% to 91.03% of the total oil composition. The dominant compounds were 1,8-cineole in E.saligna, E.urophylla, E.grandis×E.urophylla, E.grandis, E.pellita and E. dunni (67.48, 57.12, 48.21, 23.64, 20.34, 20.22%), p-Cymene in E.camaldulensis,E. dunni and E.grandis(21.32, 14.74, 13.38%), α-pinene in E.grandis and E.grandis×E.urophylla (21.77, 15.55%), α-terpinene in E. dunni and E.grandis (17.96, 9.35%), α-terpineol in E.pellita and E.saligna (19.24,11.54%), respectively. Hierarchical Cluster Analysis separated the characterized essential oils into two groups, each constituting a chemotype. E.grandis×E.urophylla and E.urophylla were classified in the same group, while E.grandis in the other.
- Published
- 2014
30. An efficient approach to the cyclotrimerisation of alkynes: solvent-free synthesis of 1,3,5-trisubstituted benzenes using p-toluenesulfonic acid monohydrate
- Author
-
Xiao-jing Feng, Yingi-ming Pan, Qi Gao, Heng-shan Wang, Fang-ping Bao, and Dian-peng Li
- Subjects
lcsh:QD241-441 ,chemistry.chemical_compound ,Solvent free ,lcsh:Organic chemistry ,chemistry ,Organic Chemistry ,p-Toluenesulfonic acid ,Organic chemistry ,Environmentally friendly ,Catalysis - Abstract
An environmentally friendly, efficient method for transforming alkynes into substituted benzenes catalyzed by p-toluenesulfonic acid monohydrate ( p-TsOH·H 2O) under solvent-free conditions has been developed, which conforms to the principles of "green" chemistry and overcomes the shortcomings of previous methods for the synthesis of substituted benzenes. The reaction is quite general and provides good to excellent yields.
- Published
- 2013
31. Fingerprint Analysis of Flos Lonicerae by High-Performance Liquid Chromatography with Evaporative Light Scattering Detection
- Author
-
Xiao Jie Yan, Jin Lei Liu, Hai Ying Jiang, Chuan Ming Fu, Feng Lai Lu, Yue Yuan Chen, and Dian Peng Li
- Subjects
Chromatography ,biology ,Formic acid ,General Engineering ,Analytical chemistry ,Flos ,biology.organism_classification ,High-performance liquid chromatography ,Light scattering ,Volumetric flow rate ,chemistry.chemical_compound ,Chlorogenic acid ,chemistry ,Fingerprint ,Chromatography detector - Abstract
A high-performance liquid chromatographic method (HPLC) with evaporative light scattering detection (ELSD) has been developed for fingerprint analysis of Flos Lonicerae. The samples were separated with an Agilent C18 column using acetonitrile (A) and 0.1% formic acid solution under gradient conditions (0→10 min:11.5%→15% A, 10→12 min:15% →22% A, 12→18min:22%→33% A; 18→30min: 33%→45% A) as the mobile phase at a flow rate of 0.8ml min−1 within 30 min. The ELSD conditions were optimized at nebulizer-gas flow rate 2.7 L min−1 and drift tube temperature 105°C. Precision experiments showed relative standard deviation (R.S.D.) of peak area and retention time were better than 2.5%, inter-day and intra-day variabilities showed that R.S.D. was ranged from 0.66% to 4.17%. Accuracy validation showed that average recovery was between 95.33% and 104.10%. The method was validated to achieve the satisfactory precision and recovery. Relative retention time and relative peak area were used to identify the common peaks for fingerprint analysis. There are nine common peaks in the fingerprint. The quality of sixteen batches of Flos Lonicerae samples was evaluated by hierarchical clustering analysis, which classfied all bacthes into two clusters. Furthermore, the contents of important medicinal compounds (chlorogenic acid, macranthoidin B and dipsacoside B) in different batches of Flos Lonicerae samples were determined simultaneously using the developed HPLC-ELSD method. The developed analytical procedure was proved to be a reliable and rapid method for the quality control of Flos Lonicerae.
- Published
- 2012
32. Quantification and Enrichment of Esculeoside a from Fresh Tomato Fruits
- Author
-
Dian Peng Li, Jian Zhang Wu, Jin Lei Liu, Feng Lai Lu, Yue Yuan Chen, and Yong Xin Wen
- Subjects
Chromatography ,biology ,Elution ,Chemistry ,Extraction (chemistry) ,General Engineering ,food and beverages ,biology.organism_classification ,High-performance liquid chromatography ,Lycopersicon ,Adsorption ,Volume (thermodynamics) ,Chromatography detector ,Pectinase - Abstract
Esculeoside A, the tomato saponins from Lycopersicon esculentum MILL, may be metabolized into various steroidal hormones such as pregnane derivatives that are expected to exhibit various bioactivities in the body, such as anti-osteoporosis, antimenopausal disorder and anti-tumor actions. In order to enrich large amount of esculeoside A for further scientific research, we firstly developed a high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) method for its quantification, and used it to examined the content of esculeoside A in different cultivated varieties of tomato fruits and tomato extract. Statistical analysis of the obtained data demonstrated that our method was simple, and has achieved desired linearity, precision and accuracy. Then we have explored the feasibility of using pectinase as a means for assisting esculeoside A extraction from fresh tomato fruits, and several factors such as the enzyme concentration, pH value of enzyme solution, temperature, incubation time which influenced on extraction were investigated. Moreover, in our previous study, the adsorption characteristics on several types of macroporous resins have been compared. Among them, D-101 resin showed the best separation behaviors on esculeoside A. So in the present paper, in order to get the best parameters for D101 adsorption, the adsorption kinetic curve, the appropriate ethanol concentration for elution and breakthrough volume were analyzed.
- Published
- 2011
33. Triterpene hexahydroxydiphenoyl esters and a quinic acid purpurogallin carbonyl ester from the leaves of Castanopsis fissa
- Author
-
Dian-Peng Li, Takashi Tanaka, Yong-Lin Huang, Yosuke Matsuo, Takaaki Tsujita, Isao Kouno, and Gen-Ichiro Nonaka
- Subjects
Purpurogallin ,Quinic Acid ,Plant Science ,Castanopsis fissa ,Horticulture ,Fagaceae ,Biochemistry ,Terpene ,chemistry.chemical_compound ,Ellagitannin ,Triterpene ,Organic chemistry ,Molecular Biology ,chemistry.chemical_classification ,Glycoside ,Esters ,General Medicine ,Quinic acid ,Galloyl quinic acid ,Triterpenes ,Plant Leaves ,Benzocycloheptenes ,chemistry ,Polyphenol ,Benzotropolone ,Myricitrin - Abstract
Triterpene hexahydroxydiphenoyl (HHDP) esters have only been isolated from Castanopsis species, and the distribution of these esters in nature is of chemotaxonomical interest. In this study, the chemical constituents of the leaves of Castanopsis fissa were examined in detail to identify and isolate potential HHDP esters. Together with 53 known compounds, 3,4-di-O-galloyl-1-O-purpurogallin carbonyl quinic acid (1) and 3,24-(S)-HHDP-2α,3β,23,24-tetrahydroxytaraxastan-28,20β-olide (2) were isolated and their structures were elucidated by spectroscopic and chemical methods. The polyphenols of the leaves were mainly composed of galloyl quinic acids, triterpenes HHDP esters, ellagitannins and flavonol glycosides. In particular, the isolation yields of 1,3,4-trigalloyl quinic acid and compound 2 were 1.53% and 0.27%, respectively, from the fresh leaves. The presence of lipid soluble HHDP esters of oleanane-type triterpenes as one of the major metabolites is an important chemotaxonomical discovery. Lipase inhibition activities and ORAC values of the major constituents were compared. The triterpene HHDP ester showed moderate lipase inhibition activity and myricitrin gave the largest ORAC value., Phytochemistry, 72(16), pp.2006-2014; 2011
- Published
- 2011
34. Based on GA Mixed with Trust Region Method Solving Nonlinear Least Square Problems
- Author
-
Miao Hu, Bo Geng, Qi Chen Wang, Tai Yong Wang, and Dian Peng Li
- Subjects
Nonlinear system ,Mathematical optimization ,Trust region ,Rate of convergence ,Computer Science::Neural and Evolutionary Computation ,Genetic algorithm ,Convergence (routing) ,General Medicine ,Rosenbrock function ,Algorithm ,Randomness ,Premature convergence ,Mathematics - Abstract
Nonlinear least square is one of the unconstrained optimization problems. In order to solve the least square trust region sub-problem, a genetic algorithm (GA) of global convergence was applied, and the premature convergence of genetic algorithms was also overcome through optimizing the search range of GA with trust region method (TRM), and the convergence rate of genetic algorithm was increased by the randomness of the genetic search. Finally, an example of banana function was established to verify the GA, and the results show the practicability and precision of this algorithm.
- Published
- 2011
35. Analysis of Natural Frequency of Simply Supported Beam with Vertical Elastic Constraint
- Author
-
Bo Geng, Miao Hu, Jin Zhou Lin, Qi Chen Wang, and Dian Peng Li
- Subjects
business.industry ,Modal analysis ,Numerical analysis ,Mathematical analysis ,Natural frequency ,General Medicine ,Structural engineering ,Finite element method ,Vibration ,Bending stiffness ,Vertical direction ,business ,Beam (structure) ,Mathematics - Abstract
Modal analysis of a simply supported beam with elastic support in vertical direction was conducted. Based on Bernoulli-Euler beam model, the first three nature frequency equations and the vibration mode function were obtained. And with different stiffness in vertical direction, the nature frequency equations were solved using numerical method, at the same time, curves of the relationship between support stiffness and nature frequency were acquired, and the corresponding functions were fitted with least square method simultaneously. Based on a typical beam structure, the accuracy of the functions was validated using finite element method, and the relative error was less than 3%.
- Published
- 2011
36. Preparative isolation and purification of five phenylethanoid glycosides from Chirita eburnea
- Author
-
Yueyuan Chen, Yongxin Wen, Dian-Peng Li, Wenjuan Chen, and Yong-Lin Huang
- Subjects
chemistry.chemical_classification ,Chromatography ,Macroporous resin ,biology ,Chemistry ,Glycoside ,Plant Science ,General Chemistry ,Phenylethanoid ,biology.organism_classification ,Isolation (microbiology) ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,Chirita - Published
- 2011
37. Studies on chemical fingerprints of Siraitia grosvenorii fruits (Luo Han Guo) by HPLC
- Author
-
Yueyuan Chen, Toshihiro Nohara, Fenglai Lu, Dian-Peng Li, Chuanming Fu, Yong-Lin Huang, Jinlei Liu, and Yongxin Wen
- Subjects
Quality Control ,Plants, Medicinal ,Chromatography ,Chemistry ,Uv spectrum ,Reference Standards ,High-performance liquid chromatography ,Cucurbitaceae ,Fruit ,Cluster Analysis ,Molecular Medicine ,Spectrophotometry, Ultraviolet ,Siraitia grosvenorii ,Retention time ,Chromatography, High Pressure Liquid ,Drugs, Chinese Herbal - Abstract
A novel, efficient, and accurate fingerprinting method using high performance liquid chromatography-photodiode array detection has been developed and optimized for the investigation and demonstration of the variance in chemical properties among Siraitia grosvenorii fruits from different origins. The effects of growth stages, cultivated varieties, collection locations, and fruit portions of the herb on chromatographic fingerprints were examined. Eleven compounds were identified on chromatograms by comparing the retention time and UV spectrum of each peak separately with those of external references. The results revealed that chromatographic fingerprints, combining similarity or hierarchical clustering analysis along with reference compounds, could efficiently identify and distinguish S. grosvenorii fruits from different sources, which provided helpful clues for studying the plants' secondary metabolites and benefitted quality control.
- Published
- 2011
38. New Pregnane Glycosides from Brucea javanica and Their Antifeedant Activity
- Author
-
Yueyuan Chen, Dian-Peng Li, Jinlei Liu, Fenglai Lu, Yong-Lin Huang, Qiao-Dan Pan, and Yongxin Wen
- Subjects
Stereochemistry ,ved/biology.organism_classification_rank.species ,Bioengineering ,Pieris rapae ,Stereoisomerism ,Biochemistry ,chemistry.chemical_compound ,Appetite Depressants ,Appetite depressants ,Brucea ,Animals ,Glycosides ,Molecular Biology ,chemistry.chemical_classification ,Larva ,Molecular Structure ,biology ,ved/biology ,Pregnane ,Glycoside ,General Chemistry ,General Medicine ,Pregnanes ,biology.organism_classification ,Brucea javanica ,chemistry ,Molecular Medicine ,Butterflies - Abstract
Three new pregnane glycosides, 3-O-β-D-glucopyranosyl-(1→2)-α-L-arabinopyranosyl-(20R)-pregn-5-ene-3β,20-diol (1), 3-O-α-L-arabinopyranosyl-(20R)-pregn-5-ene-3β,20-diol-20-O-β-D-glucopyranoside (2), 3-O-α-L-arabinopyranosyl-(20R)-pregn-5-ene-3β,20-diol-20-O-β-D-glucopyranosyl-(1→2)-β-D-glucopyranoside (3) were isolated along with four known compounds, 4-7, from the leaves and stems of Brucea javanica. Their structures were determined by detailed analyses of 1D- and 2D-NMR spectroscopic data. All of the compounds isolated from Brucea javanica were tested for the antifeedant activities against the larva of Pieris rapae. Compounds 1, 3, and 5 showed significant antifeedant activities after 72 h incubation.
- Published
- 2011
39. Research on Mechanical Structure Optimization Oriented to Dynamic Characteristics
- Author
-
Dian Peng Li, Zhi Feng Qiao, Miao Hu, Shuai Yang, and Tai Yong Wang
- Subjects
Engineering ,business.product_category ,business.industry ,Structure (category theory) ,Control engineering ,General Medicine ,Finite element method ,Machine tool ,Dynamic simulation ,Generator (circuit theory) ,Bevel gear ,Research Object ,business ,Dynamic testing - Abstract
Basic principles of dynamic design were proposed for the characteristics of machine dynamic performance. Take some curved-tooth bevel gear generator as research object. Its dynamic performance was analyzed and structure was optimized through theoretical analysis, finite element method (FEM) simulation and dynamic test analysis. Results show that these principles are suitable for dynamic performance optimization and design of machine tool.
- Published
- 2010
40. Two New Galloyl Glucosides from the Leaves of Castanopsis fordii
- Author
-
Wang Yafeng, Dian-Peng Li, Zhang-Bin Liu, Yong-Lin Huang, Li Ge, Rui-jie He, Ke-Di Yang, and Jin-Lei Liu
- Subjects
Pharmacology ,Chemistry ,Organic Chemistry ,Botany ,Analytical Chemistry ,Castanopsis fordii - Published
- 2018
41. TEM Observations of the Growth of Intermetallic Compounds at the SnBi/Cu Interface
- Author
-
Zhi-Quan Liu, Dian-Peng Li, P. J. Shang, and Jian Ku Shang
- Subjects
Materials science ,Metallurgy ,Intermetallic ,Condensed Matter Physics ,Microstructure ,Grain size ,Electronic, Optical and Magnetic Materials ,Chemical engineering ,Transmission electron microscopy ,Materials Chemistry ,Crystallite ,Electrical and Electronic Engineering ,Single crystal ,Eutectic system ,Directional solidification - Abstract
The microstructure of the eutectic SnBi/Cu interface was investigated by transmission electron microscopy to study the growth mechanisms of the intermetallic compounds (IMCs). Although the growth kinetics of the total IMC layer were similar, the individual Cu3Sn layer grew faster on polycrystalline Cu than on single-crystal substrates. It was found that, on polycrystalline Cu, newly formed Cu3Sn grains with a smaller grain size nucleated and grew at both the Cu/Cu3Sn and Cu3Sn/Cu6Sn5 interfaces during reflow and solid-state aging. The consumption of Cu6Sn5 to form Cu3Sn was faster at the Cu3Sn/Cu6Sn5 interface. While on single-crystal Cu new Cu3Sn grains nucleated only at the Cu/Cu3Sn interface, the directional growth of the initial columnar Cu3Sn controlled the advance of the Cu3Sn/Cu6Sn5 interface.
- Published
- 2009
42. Fast-growing transgenic common carp mounting compensatory growth
- Author
-
Wentao Hu, Yu-Tu Wang, Dian-Peng Li, Chao-Nan Fu, and Zhaoyi Zhu
- Subjects
Starvation ,medicine.medical_specialty ,Transgene ,Period (gene) ,Aquatic Science ,Biology ,Growth hormone ,biology.organism_classification ,Accelerated Growth ,Cyprinus ,Common carp ,Animal science ,Endocrinology ,Internal medicine ,medicine ,Compensatory growth (organism) ,medicine.symptom ,Ecology, Evolution, Behavior and Systematics - Abstract
Compensatory growth is a phase of accelerated growth apparent when favourable conditions are restored after a period of growth depression. To investigate if F-2 common 'all-fish' growth hormone gene transgenic common carp (Cyprinus carpio) could mount compensatory growth, a 9 week study at 29 degrees C was performed. The control group was fed to satiation twice a day throughout the experiment. The other two groups were deprived of feed for 1 or 2 weeks, respectively, and then fed to satiation during the re-feeding period. At the end of the experiment, the live masses of fish in the deprived groups were still significantly lower than those of the controls. During the re-feeding period, size-adjusted mean specific growth rates and mean feed intakes were significantly higher in the deprived fish than in the controls, indicating a partial compensatory growth response in these fish. No significant differences were found in food conversion efficiency between the deprived and control fish during re-feeding, suggesting that hyperphagia was the mechanism responsible for increased growth rates. The proximate composition of the deprived fish at the end of the experiment was similar to that of the control fish. This study is, to our knowledge, the first to report that fast-growing transgenic fish can achieve partial compensation of growth following starvation. (c) 2007 The Authors Journal compilation (c) 2007 The Fisheries Society of the British Isles.
- Published
- 2007
43. Growth and energy budget of F2'all-fish' growth hormone gene transgenic common carp
- Author
-
Zuoyan Zhu, Wentao Hu, Chao-Nan Fu, Yu-Sa Wang, and Dian-Peng Li
- Subjects
medicine.medical_specialty ,biology ,Metabolism ,Aquatic Science ,biology.organism_classification ,Energy budget ,Feed conversion ratio ,Cyprinus ,Common carp ,Endocrinology ,Animal science ,Excretory system ,Internal medicine ,medicine ,Dry matter ,Growth rate ,Ecology, Evolution, Behavior and Systematics - Abstract
The growth and energy budget for F-2 'all-fish' growth hormone gene transgenic common carp Cyprinus carpio of two body sizes were investigated at 29.2 degrees C for 21 days. Specific growth rate, feed intake, feed efficiency, digestibility coefficients of dry matter and protein, gross energy intake (I-E), and the proportion of I-E utilized for heat production (H-E) were significantly higher in the transgenics than in the controls. The proportion of I-E directed to waste products [faecal energy (F-E) and excretory energy loss (Z(E) + U-E) where Z(E) is through the gills and U-E through the kidney], and the proportion of metabolizable energy (M-E) for recovered energy (R-E) were significantly lower in the transgenics than in the controls. The average energy budget equation of transgenic fish was as follows: 100 I-E = 19.3 F-E + 6.0 (Z(E) + U-E) + 45.2 H-E + 29.5 R-E or 100 M-E = 60.5 H-E + 39.5 R-E. The average energy budget equation of the controls was: 100 I-E = 25.2 F-E + 7.4 (Z(E) + U-E) + 35.5 H-E + 31.9 R-E or 100 M-E = 52.7 H-E + 47.3 R-E. These findings indicate that the high growth rate of 'all-fish' transgenic common carp relative to their non-transgenic counterparts was due to their increased feed intake, reduced lose of waste productions and improved feed efficiency. The benefit of the increased energy intake by transgenic fish, however, was diminished by their increased metabolism.
- Published
- 2007
44. Cucurbitane Glycosides from Unripe Fruits of Siraitia grosvenori
- Author
-
Yongxin Wen, Tsuyoshi Ikeda, Gen-ichiro Nonaka, Tatsunori Sakamoto, Jinlei Liu, Dian-Peng Li, and Toshihiro Nohara
- Subjects
chemistry.chemical_classification ,biology ,Traditional medicine ,Stereochemistry ,Glycoside ,Tumor cells ,General Chemistry ,General Medicine ,biology.organism_classification ,Cucurbitane ,Siraitia ,chemistry.chemical_compound ,chemistry ,Triterpene ,Drug Discovery - Abstract
Studies on the constituents of the unripe fruits of Siraitia grosvenori led to the isolation of three new cucurbitane triterpene glycosides, 11-oxomogroside III (10), 11-dehydroxymogroside III (11), and 11-oxomogroside IV A (12). Their structures were determined on the basis of detailed analyses of 1D, 2D-NMR spectroscopic methods. All of the compounds isolated from the unripe fruits of S. grosvenori were tested for cytotoxic activities against tumor cells, HCT-116 and SMMC-7721.
- Published
- 2007
45. ChemInform Abstract: New Clerodane Diterpenoids from the Twigs and Leaves of Croton euryphyllus
- Author
-
De-Sheng Ning, Huang Sisi, Dian-Peng Li, Xing-De Wu, Shihong Lv, and Zheng-Hong Pan
- Subjects
Terpene ,chemistry.chemical_compound ,chemistry ,Stereochemistry ,Dimer ,General Medicine ,Croton euryphyllus ,Ring (chemistry) ,Cycloaddition ,Terpenoid ,Cyclobutane - Abstract
Three new nor-clerodane diterpenoids, crotoeurins A-C (1-3), together with four known ones were isolated from the twigs and leaves of Croton euryphyllus. Among them, crotoeurin A (1) is a new nor-clerodane diterpenoid dimer with a unique cyclobutane ring via a [2+2] cycloaddition. Their structures were elucidated by spectroscopic analyses and the stereochemistry of 1 was confirmed by single-crystal X-ray diffraction analysis. Compounds 1-3 exhibited neurite outgrowth-promoting activity on NGF-mediated PC12 cells at concentration of 10 mu M. (C) 2015 Elsevier Ltd. All rights reserved.
- Published
- 2015
46. Exploring in vitro, in vivo metabolism of mogroside V and distribution of its metabolites in rats by HPLC-ESI-IT-TOF-MS(n)
- Author
-
Ming-Ying Shang, Lei Wang, Fenglai Lu, Zhen-Cong Huang, Ru-Feng Wang, Guang-Xue Liu, Shao-Qing Cai, Feng Xu, Yong-Lin Huang, and Dian-Peng Li
- Subjects
Male ,Spectrometry, Mass, Electrospray Ionization ,Glycosylation ,Clinical Biochemistry ,Saponin ,Pharmaceutical Science ,Administration, Oral ,Hydroxylation ,Methylation ,Analytical Chemistry ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Feces ,Biotransformation ,In vivo ,Drug Discovery ,Animals ,Humans ,Tissue Distribution ,Spectroscopy ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,Chromatography ,Molecular Structure ,Metabolism ,Triterpenes ,Gastrointestinal Microbiome ,Intestines ,Metabolic pathway ,chemistry ,Biochemistry ,Mogroside ,Sweetening Agents ,Microsomes, Liver ,Drugs, Chinese Herbal - Abstract
Mogroside V, a cucurbitane-type saponin, is not only the major bioactive constituent of traditional Chinese medicine Siraitiae Fructus, but also a widely used sweetener. To clarify its biotransformation process and identify its effective forms in vivo, we studied its metabolism in a human intestinal bacteria incubation system, a rat hepatic 9000g supernatant (S9) incubation system, and rats. Meanwhile, the distribution of mogroside V and its metabolites was also reported firstly. Seventy-seven new metabolites, including 52 oxidation products formed by mono- to tetra- hydroxylation/dehydrogenation, were identified with the aid of HPLC in tandem with ESI ion trap (IT) TOF multistage mass spectrometry (HPLC-ESI-IT-TOF-MS(n)). Specifically, 14 metabolites were identified in human intestinal bacteria incubation system, 4 in hepatic S9 incubation system, 58 in faeces, 29 in urine, 14 in plasma, 34 in heart, 33 in liver, 39 in spleen, 39 in lungs, 42 in kidneys, 45 in stomach, and 51 in small intestine. The metabolic pathways of mogroside V were proposed and the identified metabolic reactions were deglycosylation, hydroxylation, dehydrogenation, isomerization, glucosylation, and methylation. Mogroside V and its metabolites were distributed unevenly in the organs of treated rats. Seven bioactive metabolites of mogroside V were identified, among which mogroside IIE was abundant in heart, liver, spleen and lung, suggesting that it may contribute to the bioactivities of mogroside V. Mogroside V was mainly excreted in urine, whereas its metabolites were mainly excreted in faeces. To our knowledge, this is the first report that a plant constituent can be biotransformed into more than 65 metabolites in vivo. These findings will improve understanding of the in vivo metabolism, distribution, and effective forms of mogroside V and congeneric molecules.
- Published
- 2015
47. Optimazation research on hydrolysis condition of walnut protein
- Author
-
Yueyuan Chen, Weibin Liang, Dian-Peng Li, Xiaojie Yan, Yang Ziming, and Jinlei Liu
- Subjects
Hydrolysis ,Computer science ,Organic chemistry - Published
- 2015
48. Enrichment and purification of phenols from Callicarpa nudiflora Hook. et Arn by HP-20 macroporous resin
- Author
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Lei Wang, Gu Zhixin, Dian-Peng Li, Huang Yonglin, Yan Xiaojie, Fenglai Lu, and Huang Sheng
- Subjects
Chromatography ,Macroporous resin ,Ethanol ,biology ,Computer science ,Elution ,biology.organism_classification ,Separation process ,Solvent ,chemistry.chemical_compound ,Adsorption ,chemistry ,Desorption ,Phenols ,Callicarpa nudiflora - Abstract
Keywords: C. nudiflora; phenols; HP-20 macroporous resin Abstract: Objective To study the technological parameters of the purification process of phenols from Callicarpa nudiflora (C. nudiflora ) with HP-20 macroporous resin.Methods The kinetic adsorption and desorption experiments were carried out on HP-20 macroporous resin (HP-20) to optimize the separation process of phenols. Additionally, the effects of four parameters including adsorption flow rate, elute flow rate, volume of 10% ethanol and 50% ethanol solution for elution were explored by a L4/3 orthogonal experiment. Finally, content of phenols in samples before and after being treated by HP-20 were compared. Results The results showed that a good separation and purification of crude total phenols extract from C. nudiflora was achieved using HP-20. The optimum conditions were found to be: initial concentration of 50 mg·mL -1 , absorb flow rate of 1 mL·min -1 , elute flow rate of 3 mL·min -1 , 5 BV of 10% ethanol solution for prewash and 10 BV of 50% ethanol solution for desorption solvent. Conclusion The content of phenols is above 40% in C. nudiflora after being treated by HP-20, indicating that HP-20 could be successfully applied to enrich and purify phenols in C. nudiflora.
- Published
- 2015
49. Cucurbitane Glycosides from Unripe Fruits of Lo Han Kuo (Siraitia grosvenori)
- Author
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Tsuyoshi Ikeda, Dian-Peng Li, Toshihiro Nohara, Nanae Matsuoka, Hourui Zhang, Gen-Ichiro Nonaka, and Tatsunori Sakamoto
- Subjects
chemistry.chemical_classification ,biology ,Traditional medicine ,Flavonoid glycosides ,Stereochemistry ,Glycoside ,General Chemistry ,General Medicine ,Mogroside V ,Cucurbitane ,biology.organism_classification ,chemistry.chemical_compound ,Siraitia ,chemistry ,Triterpene ,Mogroside ,Drug Discovery ,Kaempferol - Abstract
From the unripe fruits of Lo Han Kuo (Siraitia grosvenori), a Chinese medicinal plant, two new cucurbitane triterpene glycosides, 20-hydroxy-11-oxomogroside IA(1) (1) and 11-oxomogroside IIE (2), were isolated along with five known cucurbitane glycosides, 11-oxomogroside IA(1) (3), mogroside IIE (4), mogroside III (5), mogroside IVA (6), and mogroside V (7), and two flavonoid glycosides, kaempferol 7-O-alpha-L-rhamnopyranoside (8) and kaempferol 3,7-di-O-alpha-L-rhamnopyranoside (9). Their structures were determined on the basis of detailed analyses of 1D, 2D-NMR spectroscopic methods and by comparing with literature values. This paper describes the first investigation of unripe bitter Lo Han Kuo fruits.
- Published
- 2006
50. Identification of blapsins A and B as potent small-molecule 14-3-3 inhibitors from the insect Blaps japanensis
- Author
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Xiao-Ping Dong, Hui Guo, Yong-Ming Yan, Bernd Schneider, Lixin Zhang, Yong-Xian Cheng, Huanqin Dai, Haian Fu, Yuhong Du, and Dian-Peng Li
- Subjects
Magnetic Resonance Spectroscopy ,Stereochemistry ,media_common.quotation_subject ,Clinical Biochemistry ,Pharmaceutical Science ,Antineoplastic Agents ,Enzyme-Linked Immunosorbent Assay ,Insect ,Biochemistry ,Structure-Activity Relationship ,chemistry.chemical_compound ,Chlorocebus aethiops ,Drug Discovery ,Ic50 values ,Animals ,Structure–activity relationship ,Functional studies ,Chromans ,Molecular Biology ,Phenylacetates ,media_common ,Ethanol ,Tissue Extracts ,Chemistry ,Organic Chemistry ,Nuclear magnetic resonance spectroscopy ,Elisa assay ,Small molecule ,Coleoptera ,14-3-3 Proteins ,COS Cells ,Solvents ,Molecular Medicine - Abstract
In this study, we report three novel naturally occurring compounds, blapsins A (1) and B (2), and blapsamide (3) from the ethanol extract of the stink beetle, Blaps japanensis. The structures of these compounds were determined using spectroscopic methods. Compound 3 is a phenolic compound bearing a formamido group in the structure. Functional studies revealed that compounds 1 and 2 potently inhibited 14-3-3 protein-protein interactions (PPIs) with IC50 values of 9.2 and 10.0 mu M as determined by an ELISA assay, and 2.0 and 2.5 mu M in an FP assay, respectively. These compounds represent the first example of natural small-molecule 14-3-3 inhibitors. (C) 2012 Elsevier Ltd. All rights reserved.
- Published
- 2012
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