52 results on '"Franck Remoue"'
Search Results
2. Seasonal prevalence of Plasmodium falciparum infection and use of insecticide-treated nets among children in three agroecosystems in Aboisso, Côte d’Ivoire
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Cécile Agnimou Malanfoua Sadia-Kacou, Jean T. Coulibaly, Anne Poinsignon, Yao Tano, Maurice A Adja, Franck Remoue, Benjamin G. Koudou, Serge B. Assi, and Allassane Ouattara
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Agroecosystem ,Wet season ,Veterinary medicine ,Multivariate analysis ,General Veterinary ,biology ,business.industry ,Cash crop ,Plasmodium falciparum ,General Medicine ,Plasmodium falciparum infection ,medicine.disease ,biology.organism_classification ,Infectious Diseases ,Agriculture ,Insect Science ,parasitic diseases ,medicine ,Parasitology ,business ,Malaria - Abstract
Agroecosystems have been associated with risk of malaria. The aim of this study was to determine the relationship between three agroecosystems: (i) rubber plantation (RP); (ii) oil palm plantation (OPP); (iii) no cash crop plantation (NCCP) and the prevalence of Plasmodium falciparum infection among children living in the Aboisso region. In the three villages within (Ehania-V5) or close (N’zikro) or far from (Ayebo) to each agroecosystem (RP, OPP, and NCCP), two cross-sectional parasitological surveys were carried out during the dry and the peak of the long wet seasons. A total of 586 children aged 1–14 years were recruited in the three villages to determine the prevalence of malaria using conventional microscopy. Plasmodium falciparum was the dominant species with an overall infection prevalence of 40.8%. There was a significant difference in prevalence between agroecosystems, during both the dry (p = 0.002) and wet seasons (p
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- 2021
3. Seasonal variation and intra urban heterogeneity of the entomological risk of transmission of dengue and yellow fever in Abidjan, Côte d'Ivoire
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Négnorogo Guindo‐Coulibaly, Mintokapieu Didier Stephane Kpan, Akré Maurice Adja, Affoué Mireille Nadia Kouadio, Konan Fabrice Assouho, Dounin Danielle Zoh, Konan Rodolphe Mardoché Azongnibo, Franck Remoue, and Florence Fournet
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Dengue ,Cote d'Ivoire ,General Veterinary ,Aedes ,Insect Science ,Larva ,Yellow Fever ,Animals ,Humans ,Parasitology ,Mosquito Vectors ,Seasons ,Ecology, Evolution, Behavior and Systematics - Abstract
Dengue and yellow fever are prevalent in Côte d'Ivoire and Aedes (Stegomyia) aegypti (Linnaeus), (Diptera: Culicidae), is known as the main vector. We aimed to assess seasonal variation and spatial heterogeneity in the transmission of both arbovirus diseases in Abidjan. Entomological surveys targeting larvae of A. aegypti, were carried out between November 2015 and August 2016 covering the four climatic seasons including a cohort of 100 houses randomly selected in three neighbourhoods. A. aegypti was the predominant species (96.6%) of mosquitoes resulting from the rearing of harvested larvae, and the only vector of dengue and yellow fever recorded during the study period. The highest proportion of water storage containers (45.5%) which represented the major breeding sites infested by the larvae of A. aegypti, was observed in Anoumabo. The house indices5% and/or Breteau indices20 recorded in each neighbourhood, during the different climatic seasons, indicated that there was, a high and permanent, heterogeneity in the transmission risk of dengue and yellow fever between the three neighbourhoods. In terms of transmission risk, Anoumabo was the neighbourhood with the highest risk compared to the two others, then, particular attention should be paid to this site in terms of surveillance by vector control programme in Abidjan.
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- 2022
4. Spatial Heterogeneity and Seasonal Distribution of
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Mintokapieu Didier Stéphane, Kpan, Akré Maurice, Adja, Négnorogo, Guindo-Coulibaly, Konan Fabrice, Assouho, Affoué Mireille Nadia, Kouadio, Konan Rodolphe Mardoché, Azongnibo, Dounin Danielle, Zoh, Bi Zahouli Julien, Zahouli, Franck, Remoue, and Florence, Fournet
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Cote d'Ivoire ,Aedes ,Animals ,Mosquito Vectors ,Seasons ,Arboviruses - Abstract
Although the urban areas of Abidjan, Côte d'Ivoire have faced recurrent outbreaks of
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- 2021
5. Encyclopedia of Malaria
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Anne Poinsignon, Sylvie Cornelie, Papa M. Drame, Franck Remoue, Herbert Noukpo, Alexandra Marie, and Souleymane Doucoure
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0303 health sciences ,03 medical and health sciences ,0302 clinical medicine ,030231 tropical medicine ,Biology ,Malaria vector ,Virology ,030304 developmental biology - Published
- 2021
6. Operational Evaluation of the Effectiveness of Long-lasting Insecticidal Nets on Human-Vector Contact in an African Urban Malaria Context
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Franck Remoue, André B. Sagna, Akré M. Adja, Bertin N’Cho Tchiekoi, Christophe Rogier, Serge B. Assi, Alphonsine A. Koffi, Dipomin F. Traoré, and Mamadou Dagnogo
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Long lasting ,medicine.medical_specialty ,LLIN use ,030231 tropical medicine ,urban areas ,Psychological intervention ,Context (language use) ,Major Articles ,03 medical and health sciences ,0302 clinical medicine ,salivary biomarkers ,Environmental health ,parasitic diseases ,Anopheles ,Medicine ,030212 general & internal medicine ,biology ,business.industry ,Public health ,medicine.disease ,biology.organism_classification ,Urban malaria ,Infectious Diseases ,AcademicSubjects/MED00290 ,Oncology ,Vector (epidemiology) ,business ,gSG6-P1 ,Malaria - Abstract
Background Malaria is still a major public health concern in Côte d’Ivoire despite mass distribution of long-lasting insecticidal nets (LLINs) as a key preventive strategy. This study intended to evaluate the operational effectiveness of LLINs on the level of human-vector contact using 1 antibody-based biomarker of exposure to Anopheles in urban areas. Methods This cross-sectional study collected socio-demographic data and use of LLINs from 9 neighborhoods in the city of Bouaké (Côte d’Ivoire). Dry blood spots performed in children aged >6 months and adults were used to evaluate immunoglobulin G (IgG) responses to the Anopheles gSG6-P1 salivary peptide. Results IgG response levels to the salivary peptide were significantly lower in individuals who declared having “always” (n = 270) slept under an LLIN compared with those who had “often” (n = 2087) and “never” (n = 88) slept under an LLIN (P Conclusions The human IgG level to this gSG6-P1 salivary peptide could be a useful tool to evaluate the actual effectiveness of LLINs and help design behavioral change interventions that are crucial for sustaining universal coverage.
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- 2020
7. Pattern of antibody responses to Plasmodium falciparum antigens in individuals differentially exposed to Anopheles bites
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Kakou G, Aka, Dipomin F, Traoré, André B, Sagna, Dounin D, Zoh, Serge B, Assi, Bertin N'cho, Tchiekoi, Akré M, Adja, Franck, Remoue, and Anne, Poinsignon
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Male ,Adolescent ,Research ,Plasmodium falciparum ,Immunity ,Antibodies, Protozoan ,Infant ,Insect Bites and Stings ,Antigens, Protozoan ,Salivary proteins ,Malaria ,Exposure ,Immunomodulation ,Cote d'Ivoire ,Cross-Sectional Studies ,Child, Preschool ,parasitic diseases ,Anopheles ,Antibody Formation ,Animals ,Humans ,Female ,Malaria, Falciparum ,Child - Abstract
Background In malaria-endemic areas, human populations are frequently exposed to immunomodulatory salivary components injected during mosquito blood feeding. The consequences on pathogen-specific immune responses are not well known. This study evaluated and compared the humoral responses specific to merozoite stage vaccine candidates of Plasmodium falciparum, in children differentially exposed to Anopheles bites in a natural setting. Methods The cross-sectional study was carried out in Bouaké (Côte d’Ivoire) where entomological data and blood samples from children (0–14 years) were collected in two sites with similar malaria prevalence. Antibody (IgG, IgG1, IgG3) responses to PfAMA1 and PfMSP1 were evaluated by ELISA. Univariate and multivariate analysis were performed to assess the relationship between the immune responses to P. falciparum antigens and exposure to Anopheles bites in the total cohort and in each site, separately. The individual level of exposure to Anopheles bites was evaluated by quantifying specific IgG response to the Anopheles gSG6-P1 salivary peptide, which represents a proxy of Anopheles exposure. Results The anti-Plasmodium humoral responses were different according to the level of exposure of children, with those highly exposed to Anopheles presenting significantly lower antibody responses to PfMSP1 in total population (IgG and IgG3) and in Petessou village (IgG, IgG1, IgG3). No significant difference was seen for PfAMA1 antigen between children differently exposed to Anopheles. In Dar-es-Salam, a neighbourhood where a high Culex density was reported, children presented very low antibody levels specific to both antigens, and no difference according to the exposure to Anopheles bites was found. Conclusion These findings may suggest that immunomodulatory components of Anopheles saliva, in addition to other factors, may participate to the modulation of the humoral response specific to Plasmodium merozoite stage antigens. This epidemiological observation may form a starting point for additional work to decipher the role of mosquito saliva on the modulation of the anti-Plasmodium acquired immunity and clinical protection in combining both field and ex vivo immunological studies.
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- 2019
8. List of Contributors
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John F. Andersen, Sarah Bonnet, Nathalie Boulanger, Guy Caljon, Adriana Costero-Saint Denis, Iliano V. Coutinho-Abreu, Waldionê de Castro, Carl De Trez, Ranadhir Dey, Erol Fikrig, Stephen Higgs, Yan-Jang S. Huang, Shaden Kamhawi, Randall Kincaid, Michail Kotsyfakis, Wolfgang W. Leitner, Erin E. McClure, Hira L. Nakhasi, Sukanya Narasimhan, Fabiano Oliveira, Joao H.F. Pedra, Anne Poinsignon, Franck Remoue, José M.C. Ribeiro, Andre Sagna, Tyler R. Schleicher, Tiago D. Serafim, Dana K. Shaw, Benoît Stijlemans, Jesus G. Valenzuela, Jan Van Den Abbeele, Dana L. Vanlandingham, Esther von Stebut, Tonu Wali, and Stephen Wikel
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- 2017
9. Human IgG antibody response toAedes aegyptiNterm-34 kDa salivary peptide as an indicator to identify areas at high risk for dengue transmission: a retrospective study in urban settings of Vientiane city, Lao PDR
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Bounpone Sidavong, Audrey Dubot-Pérès, Emmanuel Elanga Ndille, François Mouchet, Florence Fournet, Sylvie Cornelie, Franck Remoue, and Souleymane Doucoure
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Adult ,Male ,Risk ,medicine.medical_specialty ,Endemic Diseases ,Urban Population ,Enzyme-Linked Immunosorbent Assay ,Aedes aegypti ,Dengue virus ,medicine.disease_cause ,Dengue fever ,Dengue ,Aedes ,medicine ,Dengue transmission ,Animals ,Humans ,Seroprevalence ,Cities ,Salivary Proteins and Peptides ,Child ,Retrospective Studies ,Gynecology ,biology ,Urbanization ,Public Health, Environmental and Occupational Health ,biology.organism_classification ,medicine.disease ,Insect Vectors ,Flavivirus ,Infectious Diseases ,Antibody response ,Laos ,Child, Preschool ,Immunoglobulin G ,Antibody Formation ,Female ,Parasitology ,Biomarkers - Abstract
Objective Using human IgG antibody response to the Aedes Nterm-34 kDa salivary peptide as an indicator of human exposure to Aedes bites in surveying exposed populations from areas at risk of dengue virus (DENV) transmission in urban settings of Vientiane city, Lao PDR. Methods Enzyme-linked immunosorbent assay tests were performed to measure the IgG response to Nterm-34 kDa peptide in blood samples collected within a flavivirus seroprevalence survey carried out in 2006 including 3558 randomly selected individuals. The level of IgG response to the Nterm-34 kDa peptide in individuals was analysed in relation to the level of urbanisation of the individual's residence, areas that presented significant differences in the prevalence of recent DENV infection. Results No differences were observed in the anti-Nterm-34 kDa IgG level between DENV-positive and DENV-negative individuals. However, the level of specific IgG response was higher among individuals living in slightly urbanised neighbourhoods than among those in more highly urbanised areas (P
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- 2014
10. First insights into the cattle serological response to tsetse salivary antigens: A promising direct biomarker of exposure to tsetse bites
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Zakaria Bengaly, Guiguigbaza-Kossigan Dayo, Bruno Bucheton, Martin Bienvenu Somda, Antoine Sanon, Anne Poinsignon, Franck Remoue, Issa Sidibé, Emilie Dama, Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])
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Veterinary medicine ,Saliva ,Time Factors ,Glossina ,Tsetse Flies ,030231 tropical medicine ,Cattle Diseases ,Context (language use) ,Biology ,Antibodies ,Serology ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Burkina Faso ,African animal trypanosomosis ,parasitic diseases ,medicine ,Animals ,Serologic Tests ,Antigens ,ComputingMilieux_MISCELLANEOUS ,Antibody ,030304 developmental biology ,2. Zero hunger ,0303 health sciences ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,General Veterinary ,Pan african ,Insect Bites and Stings ,General Medicine ,medicine.disease ,Biomarker ,Immunology ,Cattle ,Parasitology ,Seasons ,%22">Glossina ,Trypanosomiasis ,Biomarkers ,Biomarker of exposure - Abstract
In the context of the Pan African Tsetse and Trypanosomiasis Eradication Campaign, the value of tsetse saliva antibodies as a biomarker of cattle exposure to tsetse flies was evaluated, as this could provide an alternative and complementary tool to conventional entomological methods. Serum immune reactivity to Glossina (G.) palpalis (p.)gambiensis, G. tachinoides and G. morsitans (m.) submorsitans whole saliva extracts (WSE) were monitored in cattle from both tsetse free and tsetse infested areas, and in cows experimentally exposed to tsetse flies and other hematophagous arthropods. In the tsetse infested area, cattle IgG responses to Glossina WSE were significantly higher during the dry season (p < 0.0001) when herds are most exposed to tsetse flies and in infected animals (p = 0.01) as expected in the case of a biomarker of exposure. Experimental studies further confirmed this as a quick rise of specific IgGs was observed in animals exposed to tsetse flies (within weeks), followed by a rapid clearance after exposure was stopped. In contrast to the two other tsetse species, G. m. submorsitans WSE enabled to detect exposure to all tsetse species and were associated with low level of cross-reactivity to other blood sucking arthropods. Finally, IgG responses to G. m. submorsitans salivary antigens enabled to distinguish different groups of cows according to exposure levels, thus indicating that tsetse saliva antibodies are not only indicators of tsetse exposure but also are correlated to the intensity of tsetse contacts (p = 0.0031). Implementation of this new sero-epidemiological marker of cattle exposure to tsetse flies in the framework of tsetse elimination campaigns is discussed.
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- 2013
11. First screening ofAedes albopictusimmunogenic salivary proteins
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François Favier, Dorothée Missé, François Mouchet, Edith Demettre, Sylvie Cornelie, Martial Séveno, Jean Pierre Hervé, Sirilaksana Patramool, Philippe Gasque, H. Rutee, Jean-Sébastien Dehecq, Souleymane Doucoure, and Franck Remoue
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Aedes ,0303 health sciences ,Saliva ,Antigenicity ,Aedes albopictus ,biology ,Protein family ,fungi ,030231 tropical medicine ,virus diseases ,Serpin ,biology.organism_classification ,Virology ,3. Good health ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,Insect Science ,Genetics ,biology.protein ,Antibody ,Molecular Biology ,030304 developmental biology - Abstract
Study of the human antibody (Ab) response to Aedes salivary proteins can provide new biomarkers to evaluate human exposure to vector bites. The identification of genus- and/or species-specific proteins is necessary to improve the accuracy of biomarkers. We analysed Aedes albopictus immunogenic salivary proteins by 2D immunoproteomic technology and compared the profiles according to human individual exposure to Ae. albopictus or Ae. aegypti bites. Strong antigenicity to Ae. albopictus salivary proteins was detected in all individuals whatever the nature of Aedes exposure. Amongst these antigenic proteins, 68% are involved in blood feeding, including D7 protein family, adenosine deaminase, serpin and apyrase. This study provides an insight into the repertoire of Ae. albopictus immunogenic salivary proteins for the first time.
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- 2013
12. New Immuno-Epidemiological Biomarker of Human Exposure to Aedes Vector Bites: From Concept to Applications
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Franck Remoue, André B. Sagna, Mabo C. Yobo, and Emmanuel Elanga Ndille
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0301 basic medicine ,viruses ,030231 tropical medicine ,lcsh:Medicine ,salivary proteins ,Review ,Biology ,Dengue virus ,medicine.disease_cause ,Virus ,Zika virus ,Aedes exposure ,immuno-epidemiology ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Chikungunya ,Aedes ,General Immunology and Microbiology ,Transmission (medicine) ,lcsh:R ,Yellow fever ,Public Health, Environmental and Occupational Health ,virus diseases ,medicine.disease ,biology.organism_classification ,Virology ,030104 developmental biology ,Infectious Diseases ,Vector (epidemiology) ,biomarker ,arbovirus transmission - Abstract
Arthropod-borne viruses (arboviruses) such as dengue virus (DENV), chikungunya virus (CHIKV), Zika virus (ZIKV), and yellow fever virus (YFV) are the most important ‘emerging pathogens’ because of their geographic spread and their increasing impact on vulnerable human populations. To fight against these arboviruses, vector control strategies (VCS) remain one of the most valuable means. However, their implementation and monitoring are labour intensive and difficult to sustain on large scales, especially when transmission and Aedes mosquito densities are low. To increase the efficacy of VCS, current entomological methods should be improved by new complementary tools which measure the risk of arthropod-borne diseases’ transmission. The study of human–Aedes immunological relationships can provide new promising serological tools, namely antibody-based biomarkers, allowing to accurately estimate the human–Aedes contact and consequently, the risk of transmission of arboviruses and the effectiveness of VCS. This review focuses on studies highlighting the concept, techniques, and methods used to develop and validate specific candidate biomarkers of human exposure to Aedes bites. Potential applications of such antibody-based biomarkers of exposure to Aedes vector bites in the field of operational research are also discussed.
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- 2018
13. First attempt to validate the gSG6-P1 salivary peptide as an immuno-epidemiological tool for evaluating human exposure to Anopheles funestus bites
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Cheikh Sow, Souleymane Doucoure, Papa-Makhtar Drame, Anne Poinsignon, Sophie Maiga, Gilles Riveau, Franck Remoue, Emmanuel Hermann, Cheikh Thiam, Jean Biram Sarr, Ibrahima Dia, Sohidou Guindo, Lassana Konate, Sylvie Cornelie, Badara Samb, François Simondon, and François Rogerie
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0303 health sciences ,medicine.medical_specialty ,biology ,030231 tropical medicine ,Public Health, Environmental and Occupational Health ,Anopheles ,Anopheles funestus ,Environmental exposure ,Specific igg ,biology.organism_classification ,Molecular biology ,3. Good health ,03 medical and health sciences ,0302 clinical medicine ,Infectious Diseases ,Human exposure ,Tropical medicine ,Salivary Proteins ,medicine ,Parasitology ,Malaria vector ,030304 developmental biology - Abstract
Summary Objective The development of a biomarker of exposure based on the evaluation of the human antibody response specific to Anopheles salivary proteins seems promising in improving malaria control. The IgG response specific to the gSG6-P1 peptide has already been validated as a biomarker of An. gambiae exposure. This study represents a first attempt to validate the gSG6-P1 peptide as an epidemiological tool evaluating exposure to An. funestus bites, the second main malaria vector in sub-Saharan Africa. Methods A multi-disciplinary survey was performed in a Senegalese village where An. funestus represents the principal anopheline species. The IgG antibody level specific to gSG6-P1 was evaluated and compared in the same children before, at the peak and after the rainy season. Results Two-thirds of the children developed a specific IgG response to gSG6-P1 during the study period and – more interestingly – before the rainy season, when An. funestus was the only anopheline species reported. The specific IgG response increased during the An. funestus exposure season, and a positive association between the IgG level and the level of exposure to An. funestus bites was observed. Conclusions The results suggest that the evaluation of the IgG response specific to gSG6-P1 in children could also represent a biomarker of exposure to An. funestus bites. The availability of such a biomarker evaluating the exposure to both main Plasmodium falciparum vectors in Africa could be particularly relevant as a direct criterion for the evaluation of the efficacy of vector control strategies. Premiere tentative de validation du peptide salivaire gSG6-P1 comme un outil immuno-epidemiologique d’evaluation de l’exposition humaine aux piqures de An. funestus Objectif: Valider le peptide gSG6-P1 comme un outil epidemiologique evaluant l’exposition aux piqures de An. funestus, le second principal vecteur de la malaria en Afrique subsaharienne. Methodes: Etude multidisciplinaire dans un village senegalais ouAn. funestus est la principale espece d’anophele. Le taux d’anticorps IgG specifiques a gSG6-P1 a eteevalue et compare chez les memes enfants avant, durant le pic et a la fin de la saison des pluies. Resultats: Deux tiers des enfants ont developpe une reponse IgG specifique a gSG6-P1 durant la periode etudiee et - plus interessant - avant la saison des pluies, quand An. funestus etait la seule espece d’anophele signalee. La reponse IgG specifique augmentait au cours de saison d’exposition a l’An. funestus et une association positive entre le taux d’IgG et le niveau d’exposition aux piqures de An. funestus a ete observee. Conclusions: Les resultats suggerent que l’evaluation de la reponse IgG specifique a gSG6-P1 chez les enfants pourrait egalement representer un biomarqueur de l’exposition aux piqures de An. funestus. La disponibilite d’un tel biomarqueurs evaluant l’exposition aux deux principaux vecteurs de P. falciparum en Afrique pourrait etre particulierement pertinente en tant que critere pour l’evaluation directe de l’efficacite des strategies de lutte antivectorielle. Primer ensayo para validar el peptido salivar gSG6-P1 como herramienta inmuno-epidemiologica que evalue la exposicion humana frente a picaduras de An. funestus Objetivo: Validar el peptido gSG6-P1 como herramienta epidemiologica que evalue la exposicion a picaduras de An. funestus, el segundo vector de malaria mas importante en Africa sub-Sahariana. Metodos: Estudio multidisciplinar, en un poblado senegales en donde An. funestus es la principal especie anofelina. Los niveles de anticuerpos IgG especificos para gSG6-P1 se evaluaron y compararon en los mismos ninos antes, durante el pico y despues de la estacion de lluvias. Resultados: Dos tercios de los ninos desarrollaron una IgG especifica para gSG6-P1 durante el periodo de estudio y – mas interesante aun – antes de la epoca lluviosa, cuando An. funestus era la unica especie anofelina reportada. La respuesta IgG especifica aumento durante el periodo de exposicion a An. funestus y se observo una asociacion positiva entre el nivel de IgG y el nivel de exposicion a picaduras de An. funestus. Conclusiones: Los resultados sugieren que la evaluacion de la respuesta IgG especifica para gSG6-P1 en ninos tambien podria utilizarse como un biomarcador de exposicion a picaduras de An. funestus. La disponibilidad de un biomarcador que evalua la exposicion a los dos principales vectores de P. falciparum en Africa podria ser particularmente relevante como un criterio directo para la evaluacion de la eficacia de estrategias de control vectorial.
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- 2010
14. IgE and IgG4 antibody responses to Aedes saliva in African children
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Sylvie Cornelie, Badara Cisse, Cheikh Sokhna, Souleymane Doucoure, Denis Boulanger, François Mouchet, Franck Remoue, Eric Alix, and François Simondon
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Male ,Arbovirus Infections ,Climate ,Veterinary (miscellaneous) ,education ,Aedes aegypti ,Immunoglobulin E ,medicine.disease_cause ,Dengue fever ,Aedes ,parasitic diseases ,medicine ,Animals ,Humans ,Chikungunya ,Child ,Saliva ,Geography ,biology ,Yellow fever ,Infant ,medicine.disease ,biology.organism_classification ,Virology ,Insect Vectors ,Infectious Diseases ,Child, Preschool ,Immunoglobulin G ,Insect Science ,Vector (epidemiology) ,Africa ,Immunology ,biology.protein ,Female ,Parasitology - Abstract
Aedes mosquitoes are the major vectors of (re)-emerging infections including arboviruses (dengue, Chikungunya, yellow fever) in developing countries. Moreover, the emergence of Aedes-borne diseases in the developed world is currently a source of concern. Evaluation of human immune responses to Aedes bites could be a useful immuno-epidemiological tool for evaluating exposure to Aedes-borne diseases and thus predicting the risk of such emerging diseases. Specific IgE and IgG4 antibody (Ab) responses to Aedes aegypti saliva were evaluated in young Senegalese children living in an area of exposure to the Aedes vector. Specific IgE and IgG4 responses increased during rainy season of high exposure to Aedes bites. In addition, the evolution of anti-saliva isotype levels during the rainy season presented spatial heterogeneity between the studied villages. These preliminaries results support the potential approach of using anti-saliva Ab responses for evaluating exposure to Aedes vectors and risks of emerging arbovirus infections.
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- 2007
15. Schistosomiasis co-infection in humans influences inflammatory markers in uncomplicated Plasmodium falciparum malaria
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André Capron, T. O. Diallo, Olivier Garraud, Franck Remoue, Gilles Riveau, Anne-Marie Schacht, Monique Capron, Abdoulaye A. N’Diaye, Nicole Charrier, Jean-Pierre Dompnier, and S. Pillet
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Adult ,medicine.medical_specialty ,Necrosis ,Adolescent ,Plasmodium falciparum ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Schistosomiasis ,Parasitemia ,Statistics, Nonparametric ,Feces ,Schistosomiasis haematobia ,parasitic diseases ,Epidemiology ,medicine ,Animals ,Humans ,Malaria, Falciparum ,Child ,Parasite Egg Count ,Schistosoma haematobium ,biology ,Age Factors ,medicine.disease ,biology.organism_classification ,Senegal ,Cytokines ,Parasitology ,medicine.symptom ,Malaria ,Transforming growth factor - Abstract
Malaria and schistosomiasis are the two major parasite diseases present in developing countries. The epidemiological co-infection with schistosomiasis could influence the development of the physiological reaction associated with Plasmodium falciparum infection in human. Most studies have demonstrated the association of circulating levels of interferon-gamma (IFN-gamma), tumour necrosis factor-a (TNF-alpha), interleukin-10 (IL-10), transforming growth factor (TGF-beta) and soluble Tumour Necrosis Factor Receptors (sTNF-RI and sTNF-RII) with the morbidity of malaria. In the present study, we showed that Schistosoma haematobium co-infection influences, in an age-dependent manner, the unbalance between pro- and anti-inflammatory circulating cytokines that play a key role during malaria infection. Indeed, children co-infected by S. haematobium have higher levels of IFN-gamma and sTNF-RII than children infected only by P. falciparum. In contrast, co-infected adults presented a significant increase of IFN-gamma, IL-10, TGF-beta, sTNF-RI and sTNF-RII rates and IL-10/TNF-alpha ratio. Taken together, this study indicates that schistosomiasis co-infection can unbalance the regulation of inflammatory factors in uncomplicated P. falciparum malaria. The possible consequences of the schistosomiasis co-infection for age-dependent malaria morbidity are discussed.
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- 2004
16. High intraepithelial expression of estrogen and progesterone receptors in the transformation zone of the uterine cervix
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Philippe Delvenne, Franck Remoue, Nathalie Jacobs, Valerie Miot, and Jacques Boniver
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Adult ,medicine.medical_specialty ,medicine.drug_class ,Ectocervix ,Estrogen receptor ,Cervix Uteri ,Biology ,Hysterectomy ,Risk Assessment ,Sensitivity and Specificity ,Sampling Studies ,Statistics, Nonparametric ,Andrology ,Sex hormone-binding globulin ,Culture Techniques ,Internal medicine ,medicine ,Humans ,Receptor ,Probability ,Biopsy, Needle ,Obstetrics and Gynecology ,Uterine Cervical Dysplasia ,medicine.disease ,Immunohistochemistry ,Squamous metaplasia ,Cell Transformation, Neoplastic ,Endocrinology ,Receptors, Estrogen ,Estrogen ,Hormone receptor ,biology.protein ,Female ,Receptors, Progesterone ,Biomarkers ,Hormone - Abstract
Objective Because sex hormones may be involved in tumor initiation and progression, we analyzed the presence of hormone receptors in the transformation zone of the uterine cervix where the majority of human papillomavirus infections and associated (pre)neoplastic lesions develop. Study design By using 23 total hysterectomy samples from young women who underwent surgery for noncervical benign uterine disease, we analyzed, by immunohistologic techniques, the in situ expression of estrogen (E2-R) and progesterone (P4-R) receptors in the transformation zone and ectocervix of the same women. Results The expression of estrogen receptors and progesterone receptors is significantly higher in the transformation zone compared with the ectocervix. Immunohistochemical localization indicated that hormone receptor-positive cells are mainly observed in (para)basal and intermediate cell layers in both the transformation zone and ectocervical epithelium. When transformation zone samples were segregated into epithelial tissues with a predominantly mature (7/23 samples) or immature (16/23 samples) squamous metaplasia, only biopsy specimens with immature squamous metaplasia showed a significantly higher density of hormone receptor-positive cells compared with ectocervical epithelium (P Conclusion Our results suggest that the cervical transformation zone may be at increased risk of the development of cancer because of a high sensitivity to sex hormone regulation.
- Published
- 2003
17. Malaria co-infection in children influences antibody response to schistosome antigens and inflammatory markers associated with morbidity
- Author
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Nicole Charrier, Véronique Angeli, André Capron, A Ly, Anne-Marie Schacht, Maxime Hervé, Franck Remoue, Dominique De Clercq, Gilles Riveau, Jozef Vercruysse, Monique Capron, and T. O. Diallo
- Subjects
Male ,Adolescent ,Antibodies, Helminth ,Schistosomiasis ,Schistosomiasis haematobia ,Immune system ,Antigen ,Antibody Specificity ,parasitic diseases ,medicine ,Animals ,Humans ,Malaria, Falciparum ,Child ,Glutathione Transferase ,Schistosoma ,Schistosoma haematobium ,biology ,Public Health, Environmental and Occupational Health ,Plasmodium falciparum ,Helminth Proteins ,General Medicine ,biology.organism_classification ,Acquired immune system ,medicine.disease ,Virology ,Infectious Diseases ,Antigens, Helminth ,Immunology ,Cytokines ,Female ,Parasitology ,Inflammation Mediators ,Malaria - Abstract
The epidemiological coexistence of schistosomiasis and malaria is frequently observed in developing countries. Co-infection with malaria in children could influence the development of acquired immunity associated with the resistance or the pathology of schistosomiasis. In the present study, performed during May to June 1996 in Senegal, the humoral immune response to Schistosoma haematobium 28 kDa glutathione S-transferase (Sh28GST) vaccinal antigen and to soluble egg antigens (SEA) has been evaluated in individuals infected by S. haematobium. Specific immunoglobulin G3 (IgG3) and IgE responses were significantly higher in co-infected children with Plasmodium falciparum compared with children infected with S. haematobium only. In addition, circulating levels of interferon-gamma (IFN-gamma), interleukin-10 (IL-10), and soluble tumor necrosis factor receptor II (sTNF-RII), 3 parameters associated with schistosomiasis morbidity, were significantly increased in co-infected children. Taken together, this study indicated that malaria co-infection can both influence the acquired specific immune response to schistosome antigens and unbalance the regulation of inflammatory factors closely involved in schistosomiasis pathology.
- Published
- 2003
18. Functional Specific Binding of Testosterone toSchistosoma haematobium28-Kilodalton GlutathioneS-Transferase
- Author
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Anne-Marie Schacht, André Capron, Martine Pugnière, Gilles Riveau, Jean-Claude Mani, and Franck Remoue
- Subjects
medicine.medical_specialty ,medicine.drug_class ,Recombinant Fusion Proteins ,Immunology ,Microbiology ,Schistosomiasis haematobia ,Immune system ,Internal medicine ,medicine ,Animals ,Testosterone ,Functional ability ,Nippostrongylus brasiliensis ,Glutathione Transferase ,Schistosoma ,Binding Sites ,biology ,Helminth Proteins ,Androgen ,biology.organism_classification ,Infectious Diseases ,Glutathione S-transferase ,Endocrinology ,Antigens, Helminth ,Schistosoma haematobium ,biology.protein ,Parasitology ,Schistosoma mansoni ,Fungal and Parasitic Infections ,Peptides ,Protein Binding ,Hormone - Abstract
Sex hormones seem to have an influence on the level of parasitic infection. Indeed, gender-dependent patterns of prevalence and intensity of infection after puberty have been observed for several parasite species (5). It has been suggested that this effect seems to be associated with the regulatory roles of sex steroids on antiparasite immunity (2, 24). Generally, female hormones have an influence in increasing antibody response against specific antigen, which could explain the higher resistance of women against several parasitic infections (24, 25). During human Schistosoma infection, a chronic infection affecting 200 million individuals around the world, sex hormones and particularly the high level of testosterone after puberty could be an important immune modulator leading to the decrease of susceptibility to infection with age (10, 26). In mice infected by Schistosoma mansoni, fewer adult worms develop in males than in similarly infected females (9). In addition, female mice treated with testosterone before infection presented a reduction in worm burden, whereas no difference in antischistosome immune response was detected between treated and untreated animals (20). These authors suggest thus that effects of testosterone on specific immunity are not adequate to explain the differences in parasite loads observed between the sexes. Schistosomes could express a hormone receptor with homology to the testosterone receptor, which could explain the direct effect of testosterone on worm development (8). Interestingly, it has been demonstrated that testosterone treatment can affect not only the development of Nippostrongylus brasiliensis helminthic worms but also the fecundity and maturity of laid eggs (27). Glutathione S-transferases (GSTs), a family of enzymes, are able to detoxify electrophilic compounds by catalyzing the formation of glutathione conjugates (12). Mammal GSTs are also involved in the intracellular transport of a variety of endogenous metabolites, drugs, and hormones by their abilities to bind these substances (16). Particularly, GSTs are glucocorticoid-binding proteins and, thereby, may influence transport, metabolism, and action of steroids (13). It has also been demonstrated that testosterone and progesterone have the ability to bind mammal GSTs with moderate (10−6 < Kd < 10−4 M) or high (Kd < 10−6 M) affinity, respectively (16). Thus, GSTs are also involved in the transport of sexual steroids and could play a key role in the physiological action of these hormones. The Schistosoma 28-kDa GST (28GST) is an essential enzyme for the parasite life in its host and is now a vaccine candidate against schistosomiasis (6). Immunization with recombinant 28GST Schistosoma haematobium (Sh28GST) has been shown to reduce S. haematobium fecundity in experimentally infected monkeys (4). It is well established in rodents (30) and observed during human infection (11) that this antifecundity effect is associated with the inhibition of the 28GST enzymatic activity by recognation of specific antibodies. Particularly, antibodies directed against amino acids 24 to 43 or 190 to 211 involved in the enzymatic site of the 28GST inhibited the GST activity, reducing tissue egg number and egg viability (31). Ultrastructural localization of antigen in adult worms showed that 28GST was identified in the tegument and the parenchyma, but also in the germinal organ of both male and female parasites (17, 21). These results indicate that 28GST expression seems to be closely associated with parasite metabolism but also with the genital tract, which could explain the relationship between the inhibition of enzymatic activity and an antifecundity effect. The aim of our study was to demonstrate the potential binding of testosterone to Sh28GST and evaluate the functional ability of this binding on the enzymatic activity of the parasite protein.
- Published
- 2002
19. Effects of malnutrition on children's immunity to bacterial antigens in Northern Senegal
- Author
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Anne-Marie Schacht, Claire Pinçon, Fabien Herbert, Franck Remoue, André B. Sagna, Emmanuel Hermann, Mamadou Ousmane Ndiath, Gilles Riveau, Jean Biram Sarr, Lobna Gaayeb, Modou Seck, Cécile Cames, and Jean-Baptiste Hanon
- Subjects
Male ,Clostridium tetani ,Biology ,medicine.disease_cause ,complex mixtures ,Child Nutrition Disorders ,Interferon-gamma ,Immunity ,Virology ,medicine ,Tetanus Toxoid ,Humans ,Longitudinal Studies ,Child ,Antigens, Bacterial ,Tetanus ,Toxoid ,Infant ,Mycobacterium tuberculosis ,Articles ,medicine.disease ,Antibodies, Bacterial ,Senegal ,Immunity, Humoral ,Malnutrition ,Infectious Diseases ,Tetanus vaccine ,Child, Preschool ,Immunoglobulin G ,Immunology ,Humoral immunity ,Multivariate Analysis ,Cytokines ,Parasitology ,Female ,Bacterial antigen ,medicine.drug - Abstract
To evaluate immunity to vaccine-preventable diseases according to nutritional status, a longitudinal study was conducted in Senegalese children ages 1-9 years old. A linear regression analysis predicted that weight for age was positively associated with immunoglobulin G (IgG) response to tetanus toxoid in children born during the rainy season or at the beginning of the dry season. A relationship between village, time of visits, and levels of antibodies to tetanus showed that environmental factors played a role in modulating humoral immunity to tetanus vaccine over time. Moreover, a whole-blood stimulation assay highlighted that the production of interferon-gamma (IFN-gamma) in response to tetanus toxoid was compromised in stunted children. However, the absence of cytokine modulation in response to Mycobacterium tuberculosis-purified protein derivatives and phytohemagglutinin suggests that the overall ability to produce IFN-gamma was preserved in stunted children. Therefore, these results show that nutritional status can specifically alter the efficacy of long-lasting immunity to tetanus.
- Published
- 2014
20. Biomarkers of Vector Bites: Arthropod Immunogenic Salivary Proteins in Vector-Borne Diseases Control
- Author
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Alexandra Marie, Pape Drame, Sylvie Cornelie, Franck Remoue, François Mouchet, Emmanuel Elanga Ndille, Anne Poinsignon, Souleymane Doucoure, Françoise Mathieu-Daudé, Institut de Recherche pour le Développement (IRD), Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])
- Subjects
Aedes ,0303 health sciences ,biology ,Salivary gland ,Transmission (medicine) ,Anopheles gambiae ,030231 tropical medicine ,Anopheles ,biology.organism_classification ,Virology ,3. Good health ,03 medical and health sciences ,medicine.anatomical_structure ,0302 clinical medicine ,Antigen ,Vector (epidemiology) ,parasitic diseases ,Immunology ,biology.protein ,medicine ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Antibody ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology - Abstract
Vector control is one of the most valuable strategies to fight the expanding distribution of arthropod-borne diseases (ABDs). However, its implementation and monitoring are time consuming and labor intensive that may impede regular entomological survey in the field. To increase the impact of vector control strategies in fighting ABDs, the current entomological methods should be improved by new complementary tools. The exploration of the close interactions between the human host and the vector through the antigenic salivary proteins of hematophagous arthropods has lead to the development of new salivary biomarkers (SBs). This chapter focuses on the methodologies used to develop the SB of vector bites. The relevancy of these immunological biomarkers in assessing the human exposure to vector bites and their capacity to evaluate the efficacy of vector control strategies as well as the risk of pathogen transmission are also discussed. List of Abbreviations Ab Antibody ABDs Arthropod-Borne Diseases ADP Adenosine Diphosphate Ae. Aedes An. Anopheles gSG6 Anopheles gambiae Salivary Protein Ig Immunoglobulin ITN Insecticide-Treated Net kDa Kilodalton Lu. Lutzomyia P. Phlebotomus SB Salivary Biomarker SGEs Salivary Gland Extracts Th T Helper VCP Vector Control Program 1178 S. Doucoure et al.
- Published
- 2014
21. Human antibody response to Aedes albopictus salivary proteins : a potential biomarker to evaluate the efficacy of vector control in an area of chikungunya and dengue virus ransmission
- Author
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Sylvie Cornelie, Papa M. Drame, Eric D'Ortenzio, François Mouchet, Jean Sébastien DeHecq, Souleymane Doucoure, and Franck Remoue
- Subjects
Adult ,Male ,Aedes albopictus ,Adolescent ,Article Subject ,Rain ,lcsh:Medicine ,Dengue virus ,medicine.disease_cause ,Insect Control ,Arbovirus ,Antibodies ,General Biochemistry, Genetics and Molecular Biology ,Young Adult ,Aedes ,medicine ,Animals ,Humans ,Chikungunya ,Salivary Proteins and Peptides ,Aged ,General Immunology and Microbiology ,biology ,Transmission (medicine) ,lcsh:R ,General Medicine ,Middle Aged ,medicine.disease ,biology.organism_classification ,Virology ,Insect Vectors ,Vector (epidemiology) ,Immunology ,Insect Proteins ,Biomarker (medicine) ,Female ,Biomarkers ,Research Article - Abstract
Aedesborne viruses represent public health problems in southern countries and threat to emerge in the developed world. Their control is currently based on vector population control. Much effort is being devoted to develop new tools to control such arbovirus. Recent findings suggest that the evaluation of human antibody (Ab) response to arthropod salivary proteins is relevant to measuring the level of human exposure to mosquito bites. Using an immunoepidemiological approach, the present study aimed to assess the usefulness of the salivary biomarker for measuring the efficacy ofAe. albopictuscontrol strategies in La Reunion urban area. The antisaliva Ab response of adult humans exposed toAe. albopictuswas evaluatedbefore and after vector control measures. Our results showed a significant correlation between antisaliva Ab response and the level of exposure to vectors bites. The decrease ofAe.albopictusdensity has been detected by this biomarker two weeks after the implementation of control measures, suggesting its potential usefulness for evaluating control strategies in a short time period. The identification of species specific salivary proteins/peptides should improve the use of this biomarker.
- Published
- 2014
22. Anopheles Salivary Biomarker to Assess Malaria Transmission Risk Along the Thailand-Myanmar Border
- Author
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Gilles Cottrell, Daniel M. Parker, Vincent Corbel, Theeraphap Chareonviriyaphap, Anne Poinsignon, Franck Remoue, Phubeth Ya-umphan, François Nosten, Cécile Brengues, Dominique Cerqueira, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Mahidol Oxford Tropical Medicine Research Unit, University of Oxford [Oxford]-Mahidol University [Bangkok], Institut de Recherche pour le Développement (IRD), Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Vector Control Group (MIVEGEC-VCG), Kasetsart University - KU (THAILAND), and Kasetsart University (KU)
- Subjects
Male ,0301 basic medicine ,Myanmar ,law.invention ,Cohort Studies ,0302 clinical medicine ,law ,Immunology and Allergy ,Child ,Salivary biomarkers ,Malaria vector ,ComputingMilieux_MISCELLANEOUS ,response ,transmission ,Salivary Biomarker ,Anopheles ,Middle Aged ,Thailand ,3. Good health ,Infectious Diseases ,Transmission (mechanics) ,Child, Preschool ,Insect Proteins ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Biomarker (medicine) ,Female ,human antibody ,Adult ,malaria vectors ,Adolescent ,030231 tropical medicine ,Biology ,Human Immunoglobulin G ,Young Adult ,03 medical and health sciences ,Malaria transmission ,parasitic diseases ,Major Article ,medicine ,Animals ,Humans ,Salivary Proteins and Peptides ,Aged ,Infant, Newborn ,Thailand-Myanmar border ,Infant ,Insect Bites and Stings ,Environmental Exposure ,medicine.disease ,biology.organism_classification ,Malaria ,030104 developmental biology ,Immunoglobulin G ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,gSG6-P1 ,Biomarkers ,Demography - Abstract
Author(s): Ya-Umphan, Phubeth; Cerqueira, Dominique; Parker, Daniel M; Cottrell, Gilles; Poinsignon, Anne; Remoue, Franck; Brengues, Cecile; Chareonviriyaphap, Theeraphap; Nosten, Francois; Corbel, Vincent | Abstract: BackgroundThe modalities of malaria transmission along the Thailand-Myanmar border are poorly understood. Here we address the relevance of using a specific Anopheles salivary biomarker to measure the risk among humans of exposure to Anopheles bites.MethodsSerologic surveys were conducted from May 2013 to December 2014 in 4 sentinel villages. More than 9400 blood specimens were collected in filter papers from all inhabitants at baseline and then every 3 months thereafter, for up to 18 months, for analysis by enzyme-linked immunosorbent assay. The relationship between the intensity of the human antibody response and entomological indicators of transmission (human biting rates and entomological inoculation rates [EIRs]) was studied using a multivariate 3-level mixed model analysis. Heat maps for human immunoglobulin G (IgG) responses for each village and survey time point were created using QGIS 2.4.ResultsThe levels of IgG response among participants varied significantly according to village, season, and age (Pl.001) and were positively associated with the abundance of total Anopheles species and primary malaria vectors and the EIR (Pl.001). Spatial clusters of high-IgG responders were identified across space and time within study villages.ConclusionsThe gSG6-P1 biomarker has great potential to address the risk of transmission along the Thailand-Myanmar border and represents a promising tool to guide malaria interventions.
- Published
- 2016
23. Immune response to Bordetella pertussis is associated with season and undernutrition in Senegalese children
- Author
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Claire Pinçon, Cécile Cames, Emmanuel Hermann, Franck Remoue, Jean-Biram Sarr, Gilles Riveau, Modou Seck, Lobna Gaayeb, and Anne-Marie Schacht
- Subjects
Male ,Bordetella pertussis ,Nutritional Status ,Pertussis toxin ,Immune system ,Immunity ,medicine ,Humans ,Longitudinal Studies ,Child ,Pertussis Vaccine ,General Veterinary ,General Immunology and Microbiology ,biology ,Anthropometry ,Malnutrition ,Public Health, Environmental and Occupational Health ,Infant ,biology.organism_classification ,medicine.disease ,Antibodies, Bacterial ,Senegal ,Immunity, Humoral ,Vaccination ,Infectious Diseases ,Immunization ,Pertussis Toxin ,Child, Preschool ,Immunoglobulin G ,Cohort ,Immunology ,Multivariate Analysis ,Molecular Medicine ,Female ,Seasons - Abstract
Background While vaccines elicit a protective response in most recipients, studies suggest that environmental and nutritional factors can influence the strength of the individual response to immunization and to subsequent natural infectious challenges. Methods We conducted a longitudinal survey in Senegal to assess the individual response to B. pertussis, a respiratory disease against which Senegalese children are vaccinated before the age of one (Clinicaltrials.gov ID: NCT01545115). A cohort of 203 children aged 1–9 from four villages of the Senegal River Valley was followed-up for 14 months (October 2008–January 2010). During that period, four visits have been made to the villages to assess the immunological and nutritional status of these children and to determine risk factors involved in the modulation of their humoral immune response to B. pertussis toxin. Results A multivariate model has demonstrated that birth season and nutritional status appeared to modulate humoral response to pertussis toxin. Moreover, response to B. pertussis was dependent on age, village and time of visit. Conclusions These results are consistent with the hypothesis that environmental and nutritional factors modulate children's response to pertussis following natural infection or vaccination.
- Published
- 2013
24. Sex‐Dependent Neutralizing Humoral Response toSchistosoma mansoni28GST Antigen in Infected Human Populations
- Author
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Mère-Marie Diakkhate, Claude Auriault, M. Niang, Helen L. Guyatt, Anthony E. Butterworth, Franck Remoue, François Rogerie, Gilles Riveau, André Capron, J L Neyrinck, and Marie-Claire Gallissot
- Subjects
Adult ,Male ,Immunoglobulin A ,Adolescent ,Antibodies, Helminth ,Epitope ,Epitopes ,Immune system ,Antigen ,Neutralization Tests ,Animals ,Humans ,Immunology and Allergy ,Child ,Aged ,Glutathione Transferase ,Sex Characteristics ,biology ,Schistosoma mansoni ,Middle Aged ,biology.organism_classification ,Isotype ,Schistosomiasis mansoni ,Senegal ,Infectious Diseases ,Antigens, Helminth ,Immunoglobulin G ,Antibody Formation ,Immunology ,Humoral immunity ,biology.protein ,Female ,Antibody - Abstract
The reduction of Schistosoma fecundity observed after experimental vaccination with the Schistosoma mansoni 28-kDa glutathione S-transferase (Sm28GST) antigen has been related to the inhibition of glutathione S-transferase (GST) enzymatic activity by specific antibody. The humoral immune response to the protective antigen Sm28GST and to the epitopes involved in the enzymatic site (amino acid [aa] sequences 10-43 and 190-211) was evaluated in infected individuals before chemotherapy treatment. The capacity of the serum samples to inhibit GST enzymatic activity was assessed. Specific IgG3 response was predominant in the male population with a low intensity of infection and was associated with maximal GST inhibition. In contrast, the neutralizing activity of serum samples from women with a low intensity of infection was correlated with high specific IgA response specifically directed toward the 190-211 epitope. These results strongly support the hypothesis that GST-neutralizing IgG3 and IgA isotypes are sex dependent. The relationship of this specific acquired immune response with the level of intensity of infection is discussed.
- Published
- 2000
25. Cellular immune responses of a Senegalese community recently exposed to Schistosoma mansoni: correlations of infection level with age and inflammatory cytokine production by soluble egg antigen-specific cells
- Author
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M. Niang, M.-C. Gallissot, Anthony E. Butterworth, M. Marguerite, Françoise Cottrez, M. Diagne, Franck Remoue, J L Neyrinck, Isabelle Wolowczuk, Ch. Decam, Gilles Riveau, C. Moreau, M. Roberts, A. Thiam, B. Daff, M.-M. Diakkhate, André Capron, Robert F. Sturrock, Claude Auriault, J.-P. Piau, and François Rogerie
- Subjects
Adult ,Male ,Cellular immunity ,Adolescent ,medicine.medical_treatment ,Population ,Enzyme-Linked Immunosorbent Assay ,Severity of Illness Index ,Sex Factors ,Th2 Cells ,Immune system ,Antigen ,Immunity ,medicine ,Animals ,Humans ,Child ,education ,Immunity, Cellular ,education.field_of_study ,biology ,Age Factors ,Public Health, Environmental and Occupational Health ,Schistosoma mansoni ,Th1 Cells ,biology.organism_classification ,Schistosomiasis mansoni ,Senegal ,Infectious Diseases ,Cytokine ,Antigens, Helminth ,Immunology ,Cytokines ,Female ,Parasitology ,Cytokine secretion - Abstract
A recently reported epidemic of Schistosoma mansoni infection in Senegal provided an opportunity to study the dynamics of the development of immunity to human schistosomiasis. We report here on the cell-mediated immune response in a population of 99 females and 95 males, with particular emphasis on the relationship between intensity of infection and age. We found that the intensity of infection correlated negatively with age in females but not in males. In men and women, both Th1- and Th2-type cytokines were detected upon in vitro stimulation of PBMCs with soluble egg antigen (SEA) or soluble adult worm antigens (SWAP). In the female group, SEA-induced PBMC proliferation was associated with the production of IFN-gamma, IL-2 and IL-5, all of which correlated negatively with intensity of infection. Most cytokine production correlated positively with age. Spontaneous production of TNF-alpha, IL-6 and IL-10 was higher in the infected population than in an uninfected control group. Our results suggest that immunity to infection could be more pronounced in the female population and associated with a Th0/1 + 2 pattern of cytokine secretion mediated by soluble egg antigen (SEA).
- Published
- 1999
26. Local transient induction of inflammatory cytokines after intranasal administration of recombinantBordetella pertussis
- Author
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Camille Locht, André Capron, Franck Remoue, Nathalie Mielcarek, Odile Poulain-Godefroy, Raymond J. Pierce, and Gilles Riveau
- Subjects
medicine.medical_treatment ,Genetic Vectors ,Inflammation ,Biology ,Bronchoalveolar Lavage ,Microbiology ,Bordetella pertussis ,Proinflammatory cytokine ,Mice ,Immune system ,Antigen ,Transforming Growth Factor beta ,medicine ,Animals ,Secretion ,Lung ,Administration, Intranasal ,Glutathione Transferase ,Recombination, Genetic ,Mice, Inbred BALB C ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Schistosoma mansoni ,Interleukin-10 ,Infectious Diseases ,Cytokine ,Antigens, Helminth ,Humoral immunity ,Immunology ,Female ,Nasal administration ,medicine.symptom - Abstract
Inflammatory cytokines have been described to play a critical role in the orientation and amplification of the IgA immune response. In this study, we show that the intranasal administration of a Bordetella pertussis strain expressing the protective antigen glutathione-S-transferase of Schistosoma mansoni (Sm28GST) induced an inflammatory response in the lungs of mice, characterized by the production of inflammatory cytokines, such as Tumor Necrosis Factor alpha, Interleukin-6 and Transforming-Growth Factor beta. The production and the secretion of these cytokines in lung tissues were early and transient. Their presence was observed only during the first week after administration despite the persistence of the bacteria for 1 month. Two weeks after inoculation, Interleukin-10 secretion was detected in the lungs, which could explain the decrease in the production of inflammatory cytokines. These inflammation-regulating cytokines, induced in the lungs by the presence of the bacterial vector, could be part of the process generating the local immune response, in particular the anti-Sm28GST IgA response.
- Published
- 1997
27. Identification of Glossina palpalis gambiensis specific salivary antigens: towards the development of a serologic biomarker of human exposure to tsetse flies in West Africa
- Author
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Martial Séveno, Franck Remoue, Fabrice Courtin, Zakaria Bengaly, Vincent Jamonneau, Mamadou Camara, Anne Poinsignon, Philippe Solano, R. Kambiré, Edith Demettre, Martin Bienvenu Somda, Adrien Marie Gaston Belem, Sylvie Cornelie, Bruno Bucheton, Emilie Dama, Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), and Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])
- Subjects
Male ,Saliva ,Disease Vectors ,Immunoglobulin G ,Mass Spectrometry ,Salivary Glands ,Serology ,0302 clinical medicine ,African trypanosomiasis ,Electrophoresis, Gel, Two-Dimensional ,Child ,ComputingMilieux_MISCELLANEOUS ,Aged, 80 and over ,Human African trypanosomosis ,0303 health sciences ,Glossina saliva ,Middle Aged ,3. Good health ,Infectious Diseases ,Child, Preschool ,Insect Proteins ,Female ,Antibody ,Adult ,Adolescent ,Tsetse Flies ,030231 tropical medicine ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Biology ,Microbiology ,Insect bites and stings ,Antibodies ,Exposure ,03 medical and health sciences ,Young Adult ,Antigen ,parasitic diseases ,Burkina Faso ,medicine ,Animals ,Humans ,Salivary Proteins and Peptides ,030304 developmental biology ,Aged ,Insect Bites and Stings ,Biomarker ,medicine.disease ,Virology ,Vector (epidemiology) ,biology.protein ,Guinea ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Biomarkers - Abstract
The saliva of blood sucking arthropods contains a number of pharmacologically active compounds that induce an antibody response in exposed human individuals. The objectives of the present study were (i) to assess the human IgG response directed against salivary antigens of Glossina palpalis gambiensis, the main vector of Trypanosoma brucei gambiense in West Africa, as a biomarker of human tsetse contacts; and (ii) to identify specific salivary antigens. Immune reactivity of human plasma collected within active human African trypanosomiasis (HAT) foci (coastal Guinea), historical foci where tsetse flies are still present (South-West Burkina Faso) and a tsetse free area (Bobo-Dioulasso, Burkina Faso), was measured by ELISA against whole saliva extracts. In the active HAT foci and areas where tsetse flies were present in high densities, specific IgG responses were significantly higher (p < 0.0001) to those in Bobo-Dioulasso or in Loropeni, where tsetse flies were either absent or only present at low densities. Furthermore, 2D-electrophoresis combined with mass spectrometry enabled to reveal that several antigens were specifically recognized by plasma from exposed individuals. Among them, four salivary proteins were successfully identified (Ada, 5'Nuc, Ag5 and Tsgf1). These results represent a first attempt to identify Glossina salivary proteins or synthetic peptides to develop a standardized and specific biomarker of tsetse exposure in West Africa.
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- 2013
28. gSG6-P1 salivary biomarker discriminates micro-geographical heterogeneity of human exposure to Anopheles bites in low and seasonal malaria areas
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Anne Poinsignon, Franck Remoue, André B. Sagna, Ngor Faye, Mamadou Ousmane Ndiath, Gilles Riveau, Cheikh Sow, Modou Seck, Papa M. Drame, Emmanuel Hermann, Jean Biram Sarr, Lobna Gaayeb, Anne-Marie Schacht, Cheikh Sokhna, Simon Senghor, Centre de Recherche Biomédicale (CRB), Espoir Pour La Santé, Laboratoire de Parasitologie Générale, Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Département de Biologie Animale, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche sur les Maladies Infectieuses Tropicales Emergentes (URMITE), Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Vector Control Group (MIVEGEC-VCG), André Barembaye Sagna was supported by a fellowships provided by CRBEPLS and Jean Biram Sarr by a post-doctoral fellowship provided by the 'Infectiopole Sud' Foundation (Marseille, France). Lobna Gaayeb received support from the Region Nord-Pas de Calais and the Pasteur Institute of Lille. This research was supported by the Biomedical Research Center Espoir Pour La Santé (EPLS) and the Research Institute for Development (IRD-Direction de la Valorisation au Sud) with a financial participation of the FSD 'Fond Social de Développement', (BIOMARQPAL ref 2008-5901) Embassy of France in Senegal., Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), INSB-INSB-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, INSB-INSB-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), BMC, Ed., Génétique et évolution des maladies infectieuses (GEMI), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Génétique et évolution des maladies infectieuses (GEMI), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)
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Male ,Rural Population ,Entomology ,Veterinary medicine ,0302 clinical medicine ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Salivary peptide ,Longitudinal Studies ,Child ,2. Zero hunger ,0303 health sciences ,Low transmission ,biology ,Anopheles ,Senegal ,3. Good health ,Infectious Diseases ,Child, Preschool ,Anopheles exposure ,[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Biomarker (medicine) ,Insect Proteins ,Female ,Topography, Medical ,Seasons ,Adult ,medicine.medical_specialty ,030231 tropical medicine ,Insect bites and stings ,Antibodies ,03 medical and health sciences ,parasitic diseases ,medicine ,Animals ,Humans ,Salivary Proteins and Peptides ,030304 developmental biology ,Research ,Infant ,Insect Bites and Stings ,Biomarker ,medicine.disease ,biology.organism_classification ,Malaria ,Human Experimentation ,Parasitology ,Human exposure ,Immunoglobulin G ,Tropical medicine ,Immunology ,Biomarkers - Abstract
International audience; BACKGROUND: Over the past decade, a sharp decline of malaria burden has been observed in several countries. Consequently, the conventional entomological methods have become insufficiently sensitive and probably under-estimate micro-geographical heterogeneity of exposure and subsequent risk of malaria transmission. In this study, we investigated whether the human antibody (Ab) response to Anopheles salivary gSG6-P1 peptide, known as a biomarker of Anopheles exposure, could be a sensitive and reliable tool for discriminating human exposure to Anopheles bites in area of low and seasonal malaria transmission. METHODS: A multi-disciplinary survey was performed in Northern Senegal where An. gambiae s.l. is the main malaria vector. Human IgG Ab response to gSG6-P1 salivary peptide was compared according to the season and villages in children from five villages in the middle Senegal River valley, known as a low malaria transmission area. RESULTS: IgG levels to gSG6-P1 varied considerably according to the villages, discriminating the heterogeneity of Anopheles exposure between villages. Significant increase of IgG levels to gSG6-P1 was observed during the peak of exposure to Anopheles bites, and decreased immediately after the end of the exposure season. In addition, differences in the season-dependent specific IgG levels between villages were observed after the implementation of Long-Lasting Insecticidal Nets by The National Malaria Control Program in this area. CONCLUSION: The gSG6-P1 salivary peptide seems to be a reliable tool to discriminate the micro-geographical heterogeneity of human exposure to Anopheles bites in areas of very low and seasonal malaria transmission. A biomarker such as this could also be used to monitor and evaluate the possible heterogeneous effectiveness of operational vector control programs in low-exposure areas.
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- 2012
29. Lack of artemisinin resistance inPlasmodium falciparumin northwest Benin after 10 years of use of artemisinin-based combination therapy
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Didier Tchonlin, Aurore Ogouyemi-Hounto, Constance Assohou, Dorothée Kinde Gazard, Georgia Damien, Nicaise Tuikue Ndam, Atika Mama, Franck Remoue, Virgile Olivier Hounkpe, Daouda Ndiaye, Jules Doumitou Moutouama, and Awa B. Deme
- Subjects
0301 basic medicine ,Resistance ,Drug Resistance ,Drug resistance ,Plasmodium ,0302 clinical medicine ,Anti-Infective Agents ,Genotype ,Benin ,Parasite hosting ,Malaria, Falciparum ,Artemisinin ,biology ,Artemisinins ,3. Good health ,Drug Combinations ,Infectious Diseases ,Ethanolamines ,Child, Preschool ,Research Article ,medicine.drug ,Veterinary (miscellaneous) ,Plasmodium falciparum ,030106 microbiology ,030231 tropical medicine ,lcsh:Infectious and parasitic diseases ,Antimalarials ,03 medical and health sciences ,parasitic diseases ,medicine ,Humans ,lcsh:RC109-216 ,Fluorenes ,Polymorphism, Genetic ,Artemether, Lumefantrine Drug Combination ,Infant ,DNA, Protozoan ,biology.organism_classification ,medicine.disease ,Virology ,Malaria ,Insect Science ,K13 propeller ,Animal Science and Zoology ,Parasitology ,Sequence Alignment ,Nested polymerase chain reaction - Abstract
Aim: In Benin, artemisinin-based combination therapy (ACT) has been recommended as the first-line treatment for uncomplicated Plasmodium falciparum malaria since 2004. The emergence in Southeast Asia of parasites that are resistant to artemisinins poses a serious threat to global control of this disease. The presence of artemisinin resistance genotypes in parasite populations in Benin is currently unknown. The present study investigated the prevalence of relevant K13-propeller gene polymorphisms in parasite isolates from the north-western region of Benin. Method: Plasmodium falciparum isolates were collected from children with a confirmed diagnosis of malaria aged 6 months to 5 years in two towns, Cobly and Djougou, in the north-western part of Benin. The study was conducted during the rainy season from July to November 2014 in local health facilities. The K13-propeller gene was amplified in parasite isolates using nested PCR and subsequently sequenced. Results: A total of 108 children were recruited into the study. The efficiency of amplification reactions was 72% (78/108). The propeller domain of the K13 gene was successfully sequenced in 78 P. falciparum isolates; all of them were wild type with no polymorphisms detectable. Conclusion: The absence of mutations in the K13 gene indicates that P. falciparum parasite populations in the study area are still fully susceptible to artemisinins.
- Published
- 2016
30. Variation in exposure to Anopheles gambiae salivary gland peptide (gSG6-P1) across different malaria transmission settings in the western Kenya highlands
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Kingsley Badu, Franck Remoue, Guofa Zhou, John M. Ong’echa, Guiyun Yan, Bernard W. Lawson, Andrew K. Githeko, John A. Larbi, Yaw A. Afrane, and Joram Siangla
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Veterinary medicine ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,Anopheles gambiae ,Plasmodium falciparum ,030231 tropical medicine ,Population ,Antibodies, Protozoan ,Host-Parasite Interactions ,lcsh:Infectious and parasitic diseases ,law.invention ,Serology ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Seroepidemiologic Studies ,law ,Anopheles ,parasitic diseases ,Animals ,Humans ,Seroprevalence ,lcsh:RC109-216 ,Malaria, Falciparum ,Salivary Proteins and Peptides ,education ,Merozoite Surface Protein 1 ,030304 developmental biology ,0303 health sciences ,education.field_of_study ,biology ,Research ,biology.organism_classification ,Kenya ,Insect Vectors ,3. Good health ,Cross-Sectional Studies ,Infectious Diseases ,Transmission (mechanics) ,Parasitology ,Immunoglobulin G ,Epidemiological Monitoring ,Immunology ,Insect Proteins ,Seasons - Abstract
Background The existing metrics of malaria transmission are limited in sensitivity under low transmission intensity. Robust surveillance systems are needed as interventions to monitor reduced transmission and prevention of rapid reintroduction. Serological tools based on antibody responses to parasite and vector antigens are potential tools for transmission measurements. The current study sought to evaluate antibody responses to Anopheles gambiae salivary gland peptide (gSG6- P1), as a biomarker of human exposure to Anopheles bites, in different transmission settings and seasons. The comparison between anti-MSP-119 IgG immune responders and non-responders allowed exploring the robustness of the gSG6-P1 peptide as a surveillance tool in an area of decreasing malaria transmission. Methods Total IgG levels to gSG6-P1 were measured in an age-stratified cohort (< 5, 5–14 and ≥ 15 years) in a total of 1,366 participants from three localities in western Kenya [Kisii (hypoendemic), Kakamega (mesoendemic), and Kombewa (hyperendemic)] including 607 sera that were additionally tested for MSP-119 specific responses during a low and a high malaria transmission seasons. Antibody prevalence and levels were compared between localities with different transmission intensities. Regression analysis was performed to examine the association between gSG6-P1 and MSP-119 seroprevalence and parasite prevalence. Result Seroprevalence of gSG6-P1 in the uphill population was 36% while it was 50% valley bottom (χ2 = 13.2, df = 1, p
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- 2012
31. Evaluation of the effectiveness of malaria vector control measures in urban settings of Dakar by a specific anopheles salivary biomarker
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Olayidé Boussari, Richard Lalou, Stéphanie Dos Santos, Vanessa Machault, Sylvie Cornelie, Papa M. Drame, Abdoulaye Diallo, Jean-Yves LeHesran, Anne Poinsignon, Franck Remoue, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle ( MIVEGEC ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ), Santé de la mère et de l'enfant en milieu tropical : épidémiologie génétique et périnatale, Université Paris Descartes - Paris 5 ( UPD5 ), Registre Bourguignon des Cancers Digestifs, Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ) -Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ) -Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Laboratoire Population-Environnement-Développement ( LPED ), Institut de Recherche pour le Développement ( IRD ) -Aix Marseille Université ( AMU ), Laboratoire d'aérologie - LA ( LA ), Université Paul Sabatier - Toulouse 3 ( UPS ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Observatoire Midi-Pyrénées ( OMP ) -Centre National de la Recherche Scientifique ( CNRS ), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 216), Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5), Université Paris Descartes - Faculté de Pharmacie de Paris (UPD5 Pharmacie), Université Paris Descartes - Paris 5 (UPD5), Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Université d’Abomey-Calavi = University of Abomey Calavi (UAC), Laboratoire Population-Environnement-Développement (LPED), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), Laboratoire d'aérologie (LAERO), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Observatoire Midi-Pyrénées (OMP), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), Vector Control Group (MIVEGEC-VCG), ANR-07-SEST-0001,ACTU-PALU,Paludisme et diversité de l'environnement urbain africain : un enjeu majeur pour la mise en place des ACT(2007), University of Abomey Calavi (UAC), Centre National de la Recherche Scientifique (CNRS)-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Faculté de Pharmacie de Paris - Université Paris Descartes (UPD5 Pharmacie), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Mère et enfant face aux infections tropicales (MERIT - UMR_D 216), Laboratoire d'aérologie (LA), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), and ANR n°2007 SEST 001 02,SEST 001 02, ANR n°2007 SEST 001 02
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Male ,Insecticides ,Mosquito Control ,Urban Population ,Epidemiology ,lcsh:Medicine ,Global Health ,Mosquitoes ,0302 clinical medicine ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Salivary biomarkers ,lcsh:Science ,Urban areas ,Child ,Immune Response ,ComputingMilieux_MISCELLANEOUS ,0303 health sciences ,Multidisciplinary ,biology ,Anopheles ,[ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologie ,Senegal ,Socioeconomic Aspects of Health ,3. Good health ,Mosquito control ,Infectious Diseases ,Child, Preschool ,Biomarker (medicine) ,Insect Proteins ,Medicine ,Female ,Public Health ,Research Article ,Adult ,Adolescent ,Infectious Disease Control ,030231 tropical medicine ,Immunology ,Malaria vector control ,Immunoglobulins ,Insect bites and stings ,03 medical and health sciences ,Environmental health ,parasitic diseases ,medicine ,Parasitic Diseases ,Animals ,Humans ,Insecticide-Treated Bednets ,Salivary Proteins and Peptides ,Biology ,030304 developmental biology ,lcsh:R ,Immunity ,Insect Bites and Stings ,Vectors and Hosts ,biology.organism_classification ,medicine.disease ,Insect Vectors ,Malaria ,Vector (epidemiology) ,Immunoglobulin G ,Insect Repellents ,Africa ,Immunologic Techniques ,lcsh:Q ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Preventive Medicine ,Infectious Disease Modeling ,Biomarkers - Abstract
International audience; Standard entomological methods for evaluating the impact of vector control lack sensitivity in low-malaria-risk areas. The detection of human IgG specific to Anopheles gSG6-P1 salivary antigen reflects a direct measure of human-vector contact. This study aimed to assess the effectiveness of a range of vector control measures (VCMs) in urban settings by using this biomarker approach. The study was conducted from October to December 2008 on 2,774 residents of 45 districts of urban Dakar. IgG responses to gSG6-P1 and the use of malaria VCMs highly varied between districts. At the district level, specific IgG levels significantly increased with age and decreased with season and with VCM use. The use of insecticide-treated nets, by drastically reducing specific IgG levels, was by far the most efficient VCM regardless of age, season or exposure level to mosquito bites. The use of spray bombs was also associated with a significant reduction of specific IgG levels, whereas the use of mosquito coils or electric fans/air conditioning did not show a significant effect. Human IgG response to gSG6-P1 as biomarker of vector exposure represents a reliable alternative for accurately assessing the effectiveness of malaria VCM in low-malaria-risk areas. This biomarker tool could be especially relevant for malaria control monitoring and surveillance programmes in low-exposure/low-transmission settings.
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- 2012
32. Safety and immunogenicity of rSh28GST antigen in humans: phase 1 randomized clinical study of a vaccine candidate against urinary schistosomiasis
- Author
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Gilles Riveau, Anne-Marie Schacht, Franck Remoue, Michel Thiry, Dominique Deplanque, Monique Capron, André Capron, Christian Libersa, Joseph Martial, and Hubert Vodougnon
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Male ,medicine.medical_treatment ,Helminth genetics ,Pharmacology ,Schistosomiasis haematobia ,Immune Response ,Glutathione Transferase ,Schistosoma haematobium ,Vaccines, Synthetic ,Vaccines ,biology ,Immunogenicity ,lcsh:Public aspects of medicine ,Vaccination ,Helminth Proteins ,Schistosomiasis vaccine ,Healthy Volunteers ,Recombinant Proteins ,Infectious Diseases ,Tolerability ,Alum Compounds ,Cytokines ,Medicine ,Adjuvant ,medicine.drug ,Research Article ,Adult ,lcsh:Arctic medicine. Tropical medicine ,Adolescent ,Drug-Related Side Effects and Adverse Reactions ,lcsh:RC955-962 ,Immunology ,Antibodies, Helminth ,Microbiology ,Young Adult ,Antigen ,Adjuvants, Immunologic ,Neutralization Tests ,Vaccine Development ,medicine ,Animals ,Humans ,Biology ,business.industry ,Public Health, Environmental and Occupational Health ,Immunity ,lcsh:RA1-1270 ,biology.organism_classification ,Antigens, Helminth ,Parasitology ,Clinical Immunology ,business - Abstract
Background Treatment of urinary schistosomiasis by chemotherapy remains challenging due to rapid re-infection and possibly to limited susceptibility to praziquantel treatment. Therefore, therapeutic vaccines represent an attractive alternative control strategy. The objectives of this study were to assess the safety and tolerability profile of the recombinant 28 kDa glutathione S-transferase of Schistosoma haematobium (rSh28GST) in healthy volunteers, and to determine its immunogenicity. Methodology Volunteers randomly received 100 µg rSh28GST together with aluminium hydroxide (Alum) as adjuvant (n = 8), or Alum alone as a comparator (n = 8), twice with a 28-day interval between doses. A third dose of rSh28GST or Alum alone was administered to this group at day 150. In view of the results obtained, another group of healthy volunteers (n = 8) received two doses of 300 µg of rSh28GST, again with a 28-day interval. A six-month follow-up was performed with both clinical and biological evaluations. Immunogenicity of the vaccine candidate was evaluated in terms of specific antibody production, the capacity of sera to inhibit enzymatic activity of the antigen, and in vitro cytokine production. Principal Findings Among the 24 healthy male participants no serious adverse events were reported in the days or weeks after administration. Four subjects under rSh28GST reported mild reactions at the injection site while a clinically insignificant increase in bilirubin was observed in 8/24 subjects. No hematological nor biochemical evidence of toxicity was detected. Immunological analysis showed that rSh28GST was immunogenic. The induced Th2-type response was characterized by antibodies capable of inhibiting the enzymatic activity of rSh28GST. Conclusions rSh28GST in Alum did not induce any significant toxicity in healthy adults and generated a Th2-type immune response. Together with previous preclinical results, the data of safety, tolerability and quality of the specific immune response provide evidence that clinical trials with rSh28GST could be continued in humans as a potential vaccine candidate against urinary schistosomiasis., Author Summary Therapeutic vaccines represent an attractive tool in the fight against schistosomiasis. Pre-clinical immunization studies with the schistosome enzyme 28 kDa glutathione S-transferase (28GST) has been shown to significantly reduce schistosome egg production and subsequent pathology. The objective of this study was to assess the safety and immunogenicity of the recombinant 28GST of Schistosoma haematobium (rSh28GST) in healthy adult volunteers. After three administrations of 100 µg or two of 300 µg, no serious adverse events were reported in the days or weeks after each administration. Some mild adverse events were noted, including minor reactions at the injection site reported for four subjects receiving rSh28GST, but there was no hematological or biochemical evidence of toxicity. Immunological analysis showed that rSh28GST induced a consistent immune response characterized by antibodies endowed with the capacity to inhibit 28GST enzymatic activity. Present data provide evidence that clinical trials with rSh28GST could be continued in humans as a potential vaccine candidate against urinary schistosomiasis.
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- 2012
33. Differential acquisition of human antibody responses to Plasmodium falciparum according to intensity of exposure to Anopheles bites
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Emmanuel Hermann, Anne-Marie Schacht, Jean Biram Sarr, Lobna Gaayeb, Soihibou Guindo, Souleymane Doucoure, Sonia Fortin, Lassana Konate, Cheikh Sow, Franck Remoue, Gilles Riveau, André B. Sagna, Badara Samb, François Rogerie, Simon Senghor, and Ibrahima Dia
- Subjects
Male ,Plasmodium falciparum ,Schizonts ,Parasitemia ,Cohort Studies ,Immunomodulation ,Immune system ,Immunity ,Anopheles ,parasitic diseases ,medicine ,Animals ,Humans ,Malaria, Falciparum ,Child ,Antibody ,Analysis of Variance ,biology ,Public Health, Environmental and Occupational Health ,Insect Bites and Stings ,General Medicine ,Environmental Exposure ,medicine.disease ,biology.organism_classification ,Virology ,Isotype ,Senegal ,Malaria ,Infectious Diseases ,Child, Preschool ,Immunoglobulin G ,Immunology ,Antibody Formation ,Anopheles exposure ,biology.protein ,Parasitology ,Female - Abstract
a b s t r a c t Malaria immunity is modulated by many environmental and epidemiological factors. This study evaluates the influence of a hitherto unstudied environmental-epidemiological fac- tor, namely the impact of human exposure to Anopheles bites on the isotype profile of acquired antibody responses to Plasmodium falciparum. In two Senegalese villages where the intensity of exposure to Anopheles bites was markedly different (high and low exposure), specific IgG1 and IgG3 responses to P. falciparum whole schizont extract (WSE) and circum- sporozoite protein (CSP) were evaluated at the peak of Anopheles exposure (September) and later (December) in a cohort of 120 children aged 3-8 years. Multivariate analysis showed a significantly lower IgG1 response against P. falciparum WSE and CSP in children highly exposed to Anopheles bites (Gankette) compared to those who were weakly exposed (Mboula). In contrast, in both villages, parasitemia and increasing age were strongly asso- ciated with higher IgG1 and IgG3 levels. We hypothesize that high exposure to Anopheles bites could inhibit IgG1-dependent responsiveness to P. falciparum known to induce protec- tive immune responses against malaria. The impact of mosquito saliva on the regulation of specific protective immunity may need to be taken into account in epidemiological studies and trials for malaria vaccines.
- Published
- 2012
34. Assessment of exposure to Plasmodium falciparum transmission in a low endemicity area by using multiplex fluorescent microsphere-based serological assays
- Author
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Gilles Riveau, Soihibou Guindo, Christophe Rogier, Franck Remoue, Eve Orlandi-Pradines, Lassana Konate, Sonia Fortin, Cheikh Sow, Jean Biram Sarr, Sylvie Cornelie, François Rogerie, and Thierry Fusai
- Subjects
Male ,Rural Population ,Plasmodium falciparum ,Protozoan Proteins ,Antibodies, Protozoan ,Context (language use) ,Fluorescence ,lcsh:Infectious and parasitic diseases ,Serology ,law.invention ,Cohort Studies ,Antigen ,law ,parasitic diseases ,Prevalence ,Humans ,Parasite hosting ,lcsh:RC109-216 ,Serologic Tests ,Multiplex ,Malaria, Falciparum ,Child ,biology ,Research ,Infant ,biology.organism_classification ,Virology ,Senegal ,Cross-Sectional Studies ,Infectious Diseases ,Transmission (mechanics) ,Parasitology ,Child, Preschool ,Immunology ,Female - Abstract
Background The evaluation of malaria transmission intensity is a crucial indicator for estimating the burden of malarial disease. In this respect, entomological and parasitological methods present limitations, especially in low transmission areas. The present study used a sensitive multiplex assay to assess the exposure to Plasmodium falciparum infection in children living in an area of low endemicity. In three Senegalese villages, specific antibody (IgG) responses to 13 pre-erythrocytic P. falciparum peptides derived from Lsa1, Lsa3, Glurp, Salsa, Trap, Starp, Csp and Pf11.1 proteins were simultaneously evaluated before (June), at the peak (September) and after (December) the period of malaria transmission, in children aged from 1 to 8 years. Results Compared to other antigens, a high percentage of seropositivity and specific antibody levels were detected with Glurp, Salsa1, Lsa3NR2, and Lsa1J antigens. The seropositivity increased with age for all tested antigens. Specific IgG levels to Glurp, Salsa1, Lsa3NR2, and Lsa1J were significantly higher in P. falciparum infected children compared to non-infected and this increase is significantly correlated with parasite density. Conclusion The multiplex assay represents a useful technology for a serological assessment of rapid variations in malaria transmission intensity, especially in a context of low parasite rates. The use of such combined serological markers (i.e. Glurp, Lsa1, Lsa3, and Salsa) could offer the opportunity to examine these variations over time, and to evaluate the efficacy of integrated malaria control strategies.
- Published
- 2011
35. Immunoglobulin G antibody profiles against Anopheles salivary proteins in domestic animals in Senegal
- Author
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Souleymane Doucoure, Lise Grout, François Mouchet, Denis Boulanger, Abdoulaye Ngom, Sylvie Cornelie, Cheikh Sokhna, Franck Remoue, François Simondon, François Rogerie, and Gilles Riveau
- Subjects
Saliva ,Culex ,Anopheles gambiae ,Blotting, Western ,Antigens, Protozoan ,Immunoglobulin G ,Species Specificity ,domestic animals ,parasitic diseases ,Anopheles ,medicine ,antibodies ,Animals ,Salivary Proteins and Peptides ,Aedes ,saliva ,General Veterinary ,biology ,medicine.disease ,biology.organism_classification ,Virology ,Senegal ,Infectious Diseases ,Insect Science ,Animals, Domestic ,biology.protein ,Insect Proteins ,Parasitology ,Female ,Antibody ,Malaria - Abstract
Although domestic animals may not be permissive for Plasmodium, they could nevertheless play a role in the epidemiology of malaria by attracting Anopheles away from humans. To investigate interactions between domestic animals and mosquitoes, we assayed immunoglobulin G (IgG) antibodies directed against the salivary proteins of Anopheles gambiae in domestic animals living in Senegalese villages where malaria is endemic. By Western blotting, sera from bovines (n = 6), ovines (n = 36), and caprines (n = 36) did not react with Anopheles whole saliva. In contrast, equine sera recognized proteins in both saliva and salivary gland extracts. Two of the major immunogens (32 and 72 kDa) were also reactive in extracts from other major mosquito genera (Aedes and Culex), but reactions to Anopheles-specific antigens were detected in 12 of 17 horses. These data suggest that horses strongly react to Anopheles bites, and further experiments on horses are warranted to investigate the impact of this domestic animal species on the transmission of human malaria.
- Published
- 2011
36. Evaluation of the human IgG antibody response to Aedes albopictus saliva as a new specific biomarker of exposure to vector bites
- Author
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Franck Remoue, Sylvie Cornelie, Abdul Hamid Rutee, Annie Walter, Dorothée Missé, François Mouchet, Jean Sébastien DeHecq, Yelin Roca, François Favier, Jean Pierre Hervé, Philippe Gasque, Souleymane Doucoure, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Vector Control Group (MIVEGEC-VCG), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Agence Régionale de Santé Océan Indien, Agence Régionale de la Santé (ARS), CIC régional épidémiologie clinique/essais cliniques - Ile de la Réunion (CIC-EC), Institut National de la Santé et de la Recherche Médicale (INSERM), Groupe de Recherche en Immunopathologies et maladies infectueuses (GRI), Université de La Réunion (UR)-Centre hospitalier Félix-Guyon [Saint-Denis, La Réunion], and Centre Hospitalier Universitaire de La Réunion (CHU La Réunion)
- Subjects
Viral Diseases ,viruses ,medicine.disease_cause ,Dengue fever ,0302 clinical medicine ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Aedes ,Chikungunya ,0303 health sciences ,[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases ,biology ,lcsh:Public aspects of medicine ,virus diseases ,3. Good health ,Infectious Diseases ,Biomarker (medicine) ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Medicine ,Female ,Research Article ,Adult ,Aedes albopictus ,lcsh:Arctic medicine. Tropical medicine ,Adolescent ,lcsh:RC955-962 ,030231 tropical medicine ,education ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Aedes aegypti ,Arbovirus ,03 medical and health sciences ,Young Adult ,medicine ,Animals ,Humans ,Salivary Proteins and Peptides ,Saliva ,Biology ,030304 developmental biology ,Clinical Laboratory Techniques ,fungi ,Public Health, Environmental and Occupational Health ,Insect Bites and Stings ,lcsh:RA1-1270 ,Vectors and Hosts ,biology.organism_classification ,medicine.disease ,Virology ,Vector (epidemiology) ,Immunoglobulin G ,Antibody Formation ,Immunologic Techniques ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Reunion ,Biomarkers - Abstract
Background The spread of Aedes albopictus, a vector for re-emergent arbovirus diseases like chikungunya and dengue, points up the need for better control strategies and new tools to evaluate transmission risk. Human antibody (Ab) responses to mosquito salivary proteins could represent a reliable biomarker for evaluating human-vector contact and the efficacy of control programs. Methodology/Principal Findings We used ELISA tests to evaluate specific immunoglobulin G (IgG) responses to salivary gland extracts (SGE) in adults exposed to Aedes albopictus in Reunion Island. The percentage of immune responders (88%) and levels of anti-SGE IgG Abs were high in exposed individuals. At an individual level, our results indicate heterogeneity of the exposure to Aedes albopictus bites. In addition, low-level immune cross-reactivity between Aedes albopictus and Aedes aegypti SGEs was observed, mainly in the highest responders. Conclusion/Significance Ab responses to saliva could be used as an immuno-epidemiological tool for evaluating exposure to Aedes albopictus bites. Combined with entomological and epidemiological methods, a “salivary” biomarker of exposure to Aedes albopictus could enhance surveillance of its spread and the risk of arbovirus transmission, and could be used as a direct tool for the evaluation of Aedes albopictus control strategies., Author Summary Aedes-borne viruses like dengue and chikungunya are a major problem in Reunion Island. Assessing exposure to Aedes bites is crucial to estimating the risk of pathogen transmission. Currently, the exposure of populations to Aedes albopictus bites is mainly evaluated by entomological methods which are indirect and difficult to apply on a large scale. Recent findings suggest that evaluation of human antibody responses against arthropod salivary proteins could be useful in assessing exposure to mosquito bites. The results indicate that 88% of the studied population produce IgG to Ae. albopictus saliva antigens in Reunion Island and show that this biomarker can detect different levels of individual exposure. In addition, little cross-reactivity is observed with Aedes aegypti saliva, suggesting that this could be a specific marker for exposure to Aedes albopictus bites. Taken together, these results suggest that antibody responses to saliva could constitute a powerful immuno-epidemiological tool for evaluating exposure to Aedes albopictus and therefore the risk of arbovirus infection.
- Published
- 2011
37. Schistosomiasis coinfection in children influences acquired immune response against Plasmodium falciparum malaria antigens
- Author
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Anne-Marie Schacht, Dick De Clerck, Sophie Pillet, Abdoulaye A. N’Diaye, Gilles Riveau, Lobna Gaayeb, Jean-Pierre Dompnier, Nicole Charrier, T. O. Diallo, Franck Remoue, and Olivier Garraud
- Subjects
Male ,Adolescent ,Population ,Plasmodium falciparum ,Antibodies, Helminth ,lcsh:Medicine ,Antibodies, Protozoan ,Schistosomiasis ,Antigens, Protozoan ,Schistosomiasis haematobia ,Antigen ,Immunology/Immunity to Infections ,parasitic diseases ,medicine ,Animals ,Humans ,Malaria, Falciparum ,lcsh:Science ,education ,Child ,Merozoite Surface Protein 1 ,Schistosoma haematobium ,education.field_of_study ,Multidisciplinary ,biology ,lcsh:R ,medicine.disease ,biology.organism_classification ,Acquired immune system ,Virology ,Infectious Diseases ,Immunology ,Immunology/Immune Response ,Coinfection ,Cytokines ,lcsh:Q ,Malaria ,Research Article - Abstract
Background: Malaria and schistosomiasis coinfection frequently occurs in tropical countries. This study evaluates the influence of Schistosoma haematobium infection on specific antibody responses and cytokine production to recombinant merozoite surface protein-1-19 (MSP1-(19)) and schizont extract of Plasmodium falciparum in malaria-infected children. Methodology: Specific IgG1 to MSP1-(19), as well as IgG1 and IgG3 to schizont extract were significantly increased in coinfected children compared to P. falciparum mono-infected children. Stimulation with MSP1-(19) lead to a specific production of both interleukin-10 (IL-10) and interferon-gamma (IFN-gamma), whereas the stimulation with schizont extract produced an IL-10 response only in the coinfected group. Conclusions: Our study suggests that schistosomiasis coinfection favours anti-malarial protective antibody responses, which could be associated with the regulation of IL-10 and IFN-gamma production and seems to be antigen-dependent. This study demonstrates the importance of infectious status of the population in the evaluation of acquired immunity against malaria and highlights the consequences of a multiple infection environment during clinical trials of anti-malaria vaccine candidates.
- Published
- 2010
38. Impact of child malnutrition on the specific anti-Plasmodium falciparum antibody response
- Author
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Jean Biram Sarr, Kirsten B. Simondon, Franck Remoue, Gilles Riveau, Cheikh Sokhna, Florie Fillol, Denis Boulanger, and Badara Cisse
- Subjects
Male ,Rural Population ,Cross-sectional study ,Antibodies, Protozoan ,IMMUNTE ,Malaria, Falciparum ,Child ,Wasting ,education.field_of_study ,biology ,MALNUTRITION ,Senegal ,REPONSE IMMUNITAIRE ,Infectious Diseases ,ANTHROPOMETRIE NUTRITIONNELLE ,Child, Preschool ,Female ,Sample collection ,medicine.symptom ,MILIEU RURAL ,EPIDEMIOLOGIE ,lcsh:Arctic medicine. Tropical medicine ,lcsh:RC955-962 ,Plasmodium falciparum ,Population ,Antigens, Protozoan ,Enzyme-Linked Immunosorbent Assay ,Child Nutrition Disorders ,lcsh:Infectious and parasitic diseases ,parasitic diseases ,ENFANT D'AGE PRESCOLAIRE ,medicine ,Animals ,Humans ,lcsh:RC109-216 ,education ,Research ,Case-control study ,Infant ,PALUDISME ,medicine.disease ,biology.organism_classification ,Malnutrition ,Cross-Sectional Studies ,Case-Control Studies ,Immunoglobulin G ,Immunology ,Parasitology ,Malaria - Abstract
Background In sub-Saharan Africa, preschool children represent the population most vulnerable to malaria and malnutrition. It is widely recognized that malnutrition compromises the immune function, resulting in higher risk of infection. However, very few studies have investigated the relationship between malaria, malnutrition and specific immunity. In the present study, the anti-Plasmodium falciparum IgG antibody (Ab) response was evaluated in children according to the type of malnutrition. Methods Anthropometric assessment and blood sample collection were carried out during a cross-sectional survey including rural Senegalese preschool children. This cross-sectional survey was conducted in July 2003 at the onset of the rainy season. Malnutrition was defined as stunting (height-for-age P. falciparum whole extracts (schizont antigens) was assessed by ELISA in sera of the included children. Results Both the prevalence of anti-malarial immune responders and specific IgG Ab levels were significantly lower in malnourished children than in controls. Depending on the type of malnutrition, wasted children and stunted children presented a lower specific IgG Ab response than their respective controls, but this difference was significant only in stunted children (P = 0.026). This down-regulation of the specific Ab response seemed to be explained by severely stunted children (HAZ ≤ -2.5) compared to their controls (P = 0.03), while no significant difference was observed in mildly stunted children (-2.5 < HAZ P. falciparum Ab response appeared to be independent of the intensity of infection. Conclusion Child malnutrition, and particularly stunting, may down-regulate the anti-P. falciparum Ab response, both in terms of prevalence of immune responders and specific IgG Ab levels. This study provides further evidence for the influence of malnutrition on the specific anti-malarial immune response and points to the importance of taking into account child malnutrition in malaria epidemiological studies and vaccine trials.
- Published
- 2009
39. Bionomics of malaria vectors and relationship with malaria transmission and epidemiology in three physiographic zones in the Senegal River Basin
- Author
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Didier Fontenille, Mawlouth Diallo, Ibrahima Dia, Jean-Biram Sarr, Abdoulaye Diop, Gilles Riveau, Lassana Konate, Badara Samb, François Rogerie, Franck Remoue, and Malick Faye
- Subjects
Wet season ,Veterinary medicine ,Veterinary (miscellaneous) ,Anopheles gambiae ,Population ,Plasmodium falciparum ,Host-Parasite Interactions ,Bionomics ,parasitic diseases ,Anopheles ,medicine ,Gametocyte ,Prevalence ,Animals ,Humans ,Malaria, Falciparum ,education ,Child ,education.field_of_study ,biology ,Ecology ,biology.organism_classification ,medicine.disease ,Senegal ,Insect Vectors ,Infectious Diseases ,Insect Science ,Vector (epidemiology) ,Child, Preschool ,Parasitology ,Female ,Seasons ,Malaria - Abstract
Following the implementation of two dams in the Senegal River, entomological and parasitological studies were conducted in three different ecological zones in the Senegal River Basin (the low valley of Senegal River, the Guiers Lake area and the low valley of Ferlo) every 3 month in June 2004, September 2004, December 2004 and March 2005. The objective of this work was to study the influence of environmental heterogeneities on vector bionomics and malaria epidemiology. Mosquitoes were collected when landing on human volunteers and by pyrethrum spray catches. In the parasitological survey, blood samples were taken from a cohort of schoolchildren under 9 years during each entomology survey. Seven anopheline species were collected: Anopheles arabiensis, Anopheles gambiae M form, Anopheles funestus, Anopheles pharoensis, Anopheles coustani, Anopheles wellcomei and Anopheles rufipes. A. arabiensis, A. funestus and A. pharoensis were predominant in the low valley of the Senegal River, A. funestus in the Guiers Lake area and A. arabiensis in the low valley of Ferlo. Mosquito populations' dynamics varied temporally depending on the rainy season for each zone. The anthropophilic rates varied between 6 and 76% for A. gambiae s.l. and 23 and 80% for A. funestus. Only 4/396 A. pharoensis and 1/3076 A. funestus tested carried Plasmodium falciparum CS antigen. These results suggest the implication of A. pharoensis in malaria transmission. The related entomological inoculation rates were estimated to 10.44 in Mbilor and 3 infected bites in Gankette Balla and were due, respectively, to A. pharoensis and A. funestus. Overall, 1636 thick blood smears were tested from blood samples taken from schoolchildren with, respectively, a parasite and gametocyte average prevalence of 9 and 0.9%. The parasite prevalence was uniformly low in Mbilor and Gankette Balla whereas; it increased in September (16%) and then remained stable in December and March (22%) in Mboula where malaria transmission was not perceptible. However, significant differences were observed over time for parasite prevalence in Mbilor and Mboula villages whereas; it was only in Gankette Balla village where gametocyte prevalence was significantly different over time. Our study demonstrates the influence of ecological changes resulted from dams implementation in the Senegal River on the composition of vectorial system, malaria transmission and epidemiology. Such changes should be thoroughly surveyed in order to prevent any possible malaria outbreak in the Senegal River Basin.
- Published
- 2007
40. Antibody response against saliva antigens of Anopheles gambiae and Aedes aegypti in travellers in tropical Africa
- Author
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Catherine Bourgouin, Didier Fontenille, Franck Remoue, Aurélie Pascual, Laure Denis de Senneville, Denis Boulanger, Eve Orlandi-Pradines, Paul Reiter, Christophe Rogier, Sylvie Cornelie, Solenne Barbe, Claude Villard, Bruno Pradines, Kristell Penhoat, Thierry Fusai, Frédéric Pagès, Julien Bonnet, Lionel Almeras, Nicole Corre-Catelin, François Simondon, and Daniel Laffite
- Subjects
Adult ,Male ,Saliva ,Anopheles gambiae ,Molecular Sequence Data ,Immunology ,malaria ,Aedes aegypti ,Microbiology ,Arbovirus ,Antigen ,stomatognathic system ,vectors ,Aedes ,Anopheles ,parasitic diseases ,medicine ,Animals ,Humans ,Amino Acid Sequence ,Gabon ,Vector (molecular biology) ,Antigens ,Salivary Proteins and Peptides ,proteomic ,Travel ,saliva ,biology ,antibody response ,biology.organism_classification ,medicine.disease ,Virology ,Insect Vectors ,Cote d'Ivoire ,Military Personnel ,Infectious Diseases ,arbovirus ,Immunoglobulin M ,Immunoglobulin G ,travellers ,France - Abstract
Exposure to vectors of infectious diseases has been associated with antibody responses against salivary antigens of arthropods among people living in endemic areas. This immune response has been proposed as a surrogate marker of exposure to vectors appropriate for evaluating the protective efficacy of antivectorial devices. The existence and potential use of such antibody responses in travellers transiently exposed to Plasmodium or arbovirus vectors in tropical areas has never been investigated. The IgM and IgG antibody responses of 88 French soldiers against the saliva of Anopheles gambiae and Aedes aegypti were evaluated before and after a 5-month journey in tropical Africa. Antibody responses against Anopheles and Aedes saliva increased significantly in 41% and 15% of the individuals, respectively, and appeared to be specific to the mosquito genus. A proteomic and immunoproteomic analysis of anopheles and Aedes saliva allowed for the identification of some antigens that were recognized by most of the exposed individuals. These results suggest that antibody responses to the saliva of mosquitoes could be considered as specific surrogate markers of exposure of travellers to mosquito vectors that transmit arthropod borne infections.
- Published
- 2007
41. Evaluation of the antibody response to Anopheles salivary antigens as a potential marker of risk of malaria
- Author
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Cheikh Sokhna, Fatou Ba, Jean-Pierre Hervé, Franck Remoue, François Simondon, Denis Boulanger, and Badara Cisse
- Subjects
Anopheles gambiae ,Population ,Antigens, Protozoan ,Immunoglobulin G ,Antigen ,Risk Factors ,parasitic diseases ,Anopheles ,medicine ,Animals ,Humans ,Malaria, Falciparum ,education ,Child ,Saliva ,education.field_of_study ,biology ,Public Health, Environmental and Occupational Health ,Infant ,Plasmodium falciparum ,General Medicine ,biology.organism_classification ,medicine.disease ,Virology ,Senegal ,Insect Vectors ,Infectious Diseases ,Vector (epidemiology) ,Child, Preschool ,Immunology ,biology.protein ,Parasitology ,Seasons ,Malaria ,Biomarkers - Abstract
The evaluation of human immune responses to arthropod bites may be a useful marker of exposure to vector-borne diseases, with applications to malaria, the most serious parasitic infection in humans. The specific antibody (Ab) IgG response to saliva obtained from Anopheles gambiae mosquitoes was evaluated in young children from an area of seasonal malaria transmission in Senegal. Specific IgG was higher in children who developed clinical Plasmodium falciparum malaria within the 3 months that followed than in those who did not (P
- Published
- 2004
42. Specific isotype immune response in the diagnosis of human schistosomiasis pathology?
- Author
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Philippe Bonnard, Gilles Riveau, Nicole Charrier, Mamadou Diop, Franck Remoue, André Capron, Sylvie Deuffic-Burban, Eric Elguero, Jean-Pierre Dompnier, Abdoulaye Ly, Michel Cassagnou, and Anne-Marie Schacht
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Adolescent ,ANALYSE DE COHORTES ,Helminthiasis ,Antibodies, Helminth ,Schistosomiasis ,IMMUNOLOGIE ,Immunoglobulin E ,MORBIDITE ,Severity of Illness Index ,IMMUNOGLOBULINE A ,DIAGNOSTIC ,Immune system ,Antigen ,Antibody Specificity ,TEST ELISA ,Virology ,parasitic diseases ,medicine ,Animals ,Humans ,Stage (cooking) ,Child ,ANALYSE STATISTIQUE ,Aged ,Aged, 80 and over ,SCHISTOSOMIASE ,biology ,IMMUNOGLOBULINE G4 ,ISOTYPE ,Schistosoma mansoni ,Middle Aged ,biology.organism_classification ,medicine.disease ,Isotype ,Schistosomiasis mansoni ,Immunoglobulin Isotypes ,IMMUNOGLOBULINE E ,Infectious Diseases ,Antigens, Helminth ,Immunology ,biology.protein ,Parasitology ,Female ,ANTICORPS - Abstract
Since the few indirect markers available for assessing the development and the stage of intestinal schistosomiasis morbidity are weakly specific, endoscopy is still the only method able to detect severe forms of pathology. Therefore, we evaluated the isotype antibody response to the current schistosome antigen preparation (soluble egg antigens [SEA]) in 142 Senegalese patients infected with Schistosoma mansoni. They were stratified into three different stages of pathology according to ultrasonographic, endoscopic, and clinical parameters (stage 1 = no detectable pathology; stage 2 = moderate morbidity; stage 3 = severe forms of pathology). Only median specific IgG4, IgE, and IgA responses changed according to the stage of pathology. The IgA level was significantly higher in stages 2 and 3 compared with stage 1, and the IgE level was higher in stage 3 compared with stage 1. A high specific IgG4 level was observed only in stage 3 and was significantly different compared with stage 2. We show an association between the variability of the specific response to SEA and the degree of morbidity, and demonstrate that IgA and IgG4 responses could be combined markers to easily discriminate the different stages of pathology due to infection with S. mansoni.
- Published
- 2004
43. Differential production in vitro of antigen specific IgG1, IgG3 and IgA: a study in Schistosoma haematobium infected individuals
- Author
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T. O. Diallo, Ronald Perraut, Franck Remoue, Fabrice Cognasse, Lydie Béniguel, Gilles Riveau, Abdoulaye A. N’Diaye, David L. Williams, Nicole Charrier‐Mze, Olivier Garraud, and Monique Capron
- Subjects
Male ,Adolescent ,Immunology ,Antibodies, Helminth ,Peripheral blood mononuclear cell ,law.invention ,Schistosomiasis haematobia ,Antigen ,law ,Transforming Growth Factor beta ,medicine ,Animals ,Humans ,Parasite Egg Count ,B cell ,Schistosoma haematobium ,B-Lymphocytes ,biology ,Middle Aged ,biology.organism_classification ,Isotype ,In vitro ,Immunoglobulin A ,Interleukin-10 ,medicine.anatomical_structure ,Antigens, Helminth ,Immunoglobulin G ,biology.protein ,Recombinant DNA ,Leukocytes, Mononuclear ,Interleukin-2 ,Parasitology ,Female ,Antibody - Abstract
SUMMARY This study has evaluated the individual control of isotype production and the influence of external signals that can be experimentally provided in vitro, in antibody responses to two different recombinant Schistosoma antigens (Sh28GST and TPx-1). Peripheral blood mononuclear cells or enriched B cell fractions obtained from S. haematobium infected Senegalese adults were induced to terminal differentiation in vitro. The production of antibody to either antigen was donor-dependent and for each donor it was antigen-dependent. Differentiation to IgG1 and IgG3 production, and possibly IgA, specific to these conserved parasite antigens could be regulated differentially in vitro. Exogenous IL-2 and IL-10 or IL-10 and TGF-β led to the production of specific IgG3 or IgG1 and/or IgA, respectively. This is the first report on such experimentally induced differential regulation of antigen-specific IgG1 and IgG3. This may have implications in designing protocols for protein based-vaccinations aiming at eliciting antibody responses of certain protective-type isotypes.
- Published
- 2003
44. Gender-dependent specific immune response during chronic human Schistosomiasis haematobia
- Author
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Anne-Marie Schacht, Jozef Vercruysse, André Capron, O. Garraud, Gilles Riveau, Franck Remoue, A. Ly, D. To Van, and M. Picquet
- Subjects
Adult ,Male ,medicine.medical_treatment ,Immunology ,Population ,Antibodies, Helminth ,Apoptosis ,Cell Cycle Proteins ,Lymphocyte Activation ,Interferon-gamma ,Schistosomiasis haematobia ,Immune system ,Antigen ,Nuclear Matrix-Associated Proteins ,medicine ,Immunology and Allergy ,Animals ,Humans ,education ,Parasite Egg Count ,Aged ,Glutathione Transferase ,education.field_of_study ,Immunity, Cellular ,Sex Characteristics ,biology ,Tumor Necrosis Factor-alpha ,Nuclear Proteins ,Antigens, Nuclear ,Immunity to Infection ,Helminth Proteins ,T-Lymphocytes, Helper-Inducer ,Middle Aged ,Acquired immune system ,Senegal ,Immunoglobulin A ,Interleukin 10 ,Cytokine ,Antigens, Helminth ,Humoral immunity ,Chronic Disease ,biology.protein ,Schistosoma haematobium ,Female ,Antibody - Abstract
SUMMARYThe cellular and humoral acquired immune responses to Schistosoma haematobium 28 kD gluthathione S-Transferase (Sh28GST) antigen were evaluated in a Senegalese population chronically infected with S. haematobium parasite. We show a gender-dependent immune response in adult individuals presenting similar intensities of infection. Indeed, the specific IgA response and production of TGF-β and IL-10 were found significantly higher in females compared to males. In addition, we showed that this profile was combined with a weak production of Th1-related cytokines (TNFα and IFNγ) and was associated with an absence of proliferation to the antigen. A significantly higher Nuclear Matrix Protein 41/7 secretion, an apoptosis marker, was specifically observed in mononuclear blood cell cultures of females suggesting that a specific cell death process was engaged in a gender-dependent manner. This specific profile could be associated with the so-called T helper type-3 (Th3) immune response specifically promoting the production of IgA and would be developed upon the down-regulation of the specific Type-1 response by a probable cell death mechanism. This gender-dependent immune regulation, which may be under the influence of nonimmunological factors like sexual hormones, may be related to the chronicity of the infection.
- Published
- 2001
45. Homologous and heterologous protection after single intranasal administration of live attenuated recombinant Bordetella pertussis
- Author
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Andre Capron, Rudy Antoine, Camille Locht, Franck Remoue, Gilles Riveau, and Nathalie Mielcarek
- Subjects
Bordetella pertussis ,Whooping Cough ,Biomedical Engineering ,Filamentous haemagglutinin adhesin ,Heterologous ,Bioengineering ,Pertussis toxin ,Applied Microbiology and Biotechnology ,Microbiology ,Mice ,Animals ,Virulence Factors, Bordetella ,Adhesins, Bacterial ,Administration, Intranasal ,Antigens, Bacterial ,biology ,Immunogenicity ,Schistosoma mansoni ,biology.organism_classification ,Virology ,Schistosomiasis mansoni ,Hemagglutinins ,Immunization ,Pertussis Toxin ,Antibody Formation ,Bacterial Vaccines ,Molecular Medicine ,Nasal administration ,Female ,Biotechnology - Abstract
While single-dose mucosal immunization is best achieved by the use of attenuated live microorganisms, attenuation generally results in decreased immunogenicity. We attenuated Bordetella pertussis by the deletion of the pertussis toxin gene. A single intranasal administration of this strain protected against subsequent challenge as well as did the parent strain and better than immunization with commercial vaccine. Unexpectedly, this attenuation resulted in increased immunogenicity against the protective antigen filamentous hemagglutinin (FHA). In addition, immunogenicity was also enhanced against the Schistosoma mansoni Sm28GST genetically fused to FHA, resulting in protection against the parasite, as characterized by a reduction in worm burden and egg charge, after a single intranasal administration. Thus, attenuated recombinant B. pertussis strains are promising vectors for the simultaneous protection against pertussis and heterologous diseases by a single intranasal administration.
- Published
- 1998
46. Association between Serum Cytokine Profiles and Schistosomiasis‐Related Hepatic Fibrosis: Infection bySchistosoma japonicumversusS. mansoni
- Author
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Anne-Marie Schacht, Philippe Bonnard, Franck Remoue, Gilles Pialoux, and Gilles Riveau
- Subjects
Serum cytokine ,Infectious Diseases ,biology ,business.industry ,Schistosoma japonicum ,Immunology ,medicine ,Immunology and Allergy ,Schistosomiasis ,Hepatic fibrosis ,medicine.disease ,biology.organism_classification ,business - Published
- 2006
47. In Silico Identification of a Candidate Synthetic Peptide (Tsgf118–43) to Monitor Human Exposure to Tsetse Flies in West Africa
- Author
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Sylvie Cornelie, Philippe Solano, Bruno Bucheton, Adrien Marie Gaston Belem, Vincent Jamonneau, Anne Poinsignon, Emilie Dama, Martin Bienvenu Somda, Hamidou Ilboudo, Zakaria Bengaly, Franck Remoue, Emmanuel Elanga Ndille, Mamadou Camara, Centre international de recherche-développement sur l'élevage en zone sub-humide (CIRDES), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre de Recherche Entomologique de Cotonou (CREC), Ministère de la Santé, Programme National de Lutte contre la Trypanosomose Humaine Africaine, Ministère de la Santé et de l’Hygiène Publique, Transmission-Interactions-Adaptations hôtes/vecteurs/pathogènes (MIVEGEC-TRIAD), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Interaction hôtes vecteurs parasites dans les infections par des trypanosomatidae (UMR InterTryp ), Institut de Recherche pour le Développement (IRD)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Université Polytechnique Nazi Boni Bobo-Dioulasso (UNB), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB), and Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)
- Subjects
Male ,Saliva ,Immunoglobulin G ,fluids and secretions ,0302 clinical medicine ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,African trypanosomiasis ,Child ,Aged, 80 and over ,Immunoassay ,2. Zero hunger ,0303 health sciences ,biology ,lcsh:Public aspects of medicine ,Middle Aged ,3. Good health ,Africa, Western ,Infectious Diseases ,Child, Preschool ,Insect Proteins ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Biomarker (medicine) ,Female ,Antibody ,Research Article ,Adult ,lcsh:Arctic medicine. Tropical medicine ,Adolescent ,Tsetse Flies ,lcsh:RC955-962 ,In silico ,030231 tropical medicine ,Computational biology ,Antibodies ,Young Adult ,03 medical and health sciences ,stomatognathic system ,Antigen ,parasitic diseases ,medicine ,Animals ,Humans ,Salivary Proteins and Peptides ,Aged ,030304 developmental biology ,Diagnostic Tests, Routine ,Public Health, Environmental and Occupational Health ,Infant ,Insect Bites and Stings ,Tsetse fly ,lcsh:RA1-1270 ,biology.organism_classification ,medicine.disease ,Immunology ,biology.protein ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie - Abstract
Background The analysis of humoral responses directed against the saliva of blood-sucking arthropods was shown to provide epidemiological biomarkers of human exposure to vector-borne diseases. However, the use of whole saliva as antigen presents several limitations such as problems of mass production, reproducibility and specificity. The aim of this study was to design a specific biomarker of exposure to tsetse flies based on the in silico analysis of three Glossina salivary proteins (Ada, Ag5 and Tsgf1) previously shown to be specifically recognized by plasma from exposed individuals. Methodology/Principal Findings Synthetic peptides were designed by combining several linear epitope prediction methods and Blast analysis. The most specific peptides were then tested by indirect ELISA on a bank of 160 plasma samples from tsetse infested areas and tsetse free areas. Anti-Tsgf118–43 specific IgG levels were low in all three control populations (from rural Africa, urban Africa and Europe) and were significantly higher (p, Author Summary Increasing interest is paid to blood-sucking arthropod's salivary antigens to develop host direct biomarkers of exposure. Nevertheless use of whole saliva is problematic both because of mass production and specificity issues. Here, we describe an in silico approach we used to identify potential epitopes on the amino acid sequence of three tsetse salivary proteins (Ada, Ag5 and Tsgf1) that were previously shown to be specifically recognized by antibodies from exposed individuals. Three candidate peptides were synthesized and evaluated on a set of plasma collected in different tsetse-infested and tsetse-free areas. The Tsgf118–43 synthetic peptide appeared as a promising candidate to assess human exposure to tsetse flies as antibody responses were low in all three control groups and were significantly higher in our two exposed groups. Significantly higher anti- Tsgf118–43 responses were also observed in sleeping sickness patients as compared to uninfected controls suggesting that Tsgf118–43 may be used both to assess human tsetse contacts and the risk of infection by trypanosomes. This new sero-epidemiological tool could thus help National Control Programs to quickly map human exposure levels in order to better target vector control efforts and monitor vector control efficiency.
- Published
- 2013
48. Relationship between Exposure to Vector Bites and Antibody Responses to Mosquito Salivary Gland Extracts
- Author
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Ibrahima Diouf, Thierry Fusai, Lionel Almeras, Christophe Rogier, Frédéric Pagès, Eve Orlandi-Pradines, Aurélie Pascual, Albin Fontaine, and Franck Remoue
- Subjects
Male ,lcsh:Medicine ,Mosquitoes ,Population density ,Salivary Glands ,Immunoglobulin G ,Serology ,lcsh:Science ,Immune Response ,Multidisciplinary ,biology ,Salivary gland ,Middle Aged ,Infectious Diseases ,medicine.anatomical_structure ,Medicine ,Insect Proteins ,Female ,France ,Research Article ,Immunology ,Immunoglobulins ,Aedes aegypti ,Insect bites and stings ,Immunomodulation ,Species Specificity ,medicine ,Animals ,Humans ,Salivary Proteins and Peptides ,Biology ,Aedes ,Tissue Extracts ,lcsh:R ,Immunity ,Immunoregulation ,Insect Bites and Stings ,Vectors and Hosts ,biology.organism_classification ,medicine.disease ,Insect Vectors ,Kinetics ,Culicidae ,Immune System ,Vector (epidemiology) ,Humoral Immunity ,Antibody Formation ,biology.protein ,lcsh:Q - Abstract
Mosquito-borne diseases are major health problems worldwide. Serological responses to mosquito saliva proteins may be useful in estimating individual exposure to bites from mosquitoes transmitting these diseases. However, the relationships between the levels of these IgG responses and mosquito density as well as IgG response specificity at the genus and/or species level need to be clarified prior to develop new immunological markers to assess human/vector contact. To this end, a kinetic study of antibody levels against several mosquito salivary gland extracts from southeastern French individuals living in three areas with distinct ecological environments and, by implication, distinct Aedes caspius mosquito densities were compared using ELISA. A positive association was observed between the average levels of IgG responses against Ae. caspius salivary gland extracts and spatial Ae. caspius densities. Additionally, the average level of IgG responses increased significantly during the peak exposure to Ae. caspius at each site and returned to baseline four months later, suggesting short-lived IgG responses. The species-specificity of IgG antibody responses was determined by testing antibody responses to salivary gland extracts from Cx. pipiens, a mosquito that is present at these three sites at different density levels, and from two other Aedes species not present in the study area (Ae. aegypti and Ae. albopictus). The IgG responses observed against these mosquito salivary gland extracts contrasted with those observed against Ae. caspius salivary gland extracts, supporting the existence of species-specific serological responses. By considering different populations and densities of mosquitoes linked to environmental factors, this study shows, for the first time, that specific IgG antibody responses against Ae. caspius salivary gland extracts may be related to the seasonal and geographical variations in Ae. caspius density. Characterisation of such immunological-markers may allow the evaluation of the effectiveness of vector-control strategies or estimation of the risk of vector-borne disease transmission.
- Published
- 2011
49. Differential Expression of Salivary Proteins between Susceptible and Insecticide-Resistant Mosquitoes of Culex quinquefasciatus
- Author
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Martial Séveno, Marie Rossignol, Vincent Corbel, Franck Remoue, Sylvie Cornelie, Edith Demettre, and Innocent Djègbè
- Subjects
Proteomics ,Proteome ,Culex ,lcsh:Medicine ,Gene Expression ,Forms of Evolution ,Biochemistry ,Mass Spectrometry ,Filariasis ,Insecticide Resistance ,Chemical Biology ,parasitic diseases ,Genetics ,medicine ,Animals ,Electrophoresis, Gel, Two-Dimensional ,Salivary Proteins and Peptides ,Allele ,lcsh:Science ,Biology ,Genome Evolution ,Pathogen ,Evolutionary Biology ,Multidisciplinary ,biology ,Transmission (medicine) ,lcsh:R ,fungi ,Proteins ,Genomics ,Comparative Genomics ,medicine.disease ,biology.organism_classification ,Culex quinquefasciatus ,Functional Genomics ,Vector (epidemiology) ,lcsh:Q ,PEST analysis ,Zoology ,Entomology ,Research Article - Abstract
BACKGROUND: The Culex quinquefasciatus mosquito, a major pest and vector of filariasis and arboviruses in the tropics, has developed multiple resistance mechanisms to the main insecticide classes currently available in public health. Among them, the insensitive acetylcholinesterase (ace-1(R) allele) is widespread worldwide and confers cross-resistance to organophosphates and carbamates. Fortunately, in an insecticide-free environment, this mutation is associated with a severe genetic cost that can affect various life history traits. Salivary proteins are directly involved in human-vector contact during biting and therefore play a key role in pathogen transmission. METHODS AND RESULTS: An original proteomic approach combining 2D-electrophoresis and mass spectrometry was adopted to compare the salivary expression profiles of two strains of C. quinquefasciatus with the same genetic background but carrying either the ace-1(R) resistance allele or not (wild type). Four salivary proteins were differentially expressed (>2 fold, P
- Published
- 2011
50. The bordetella pertussis filamentous h˦magglutinin enhances immunogenicity of sm28GST in liposomes
- Author
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Anne-Marie Schacht, G. Riveau, N. Mielcarek, N. Ivanoff, André Capron, Camille Locht, O. Poulain-Godefroy, and Franck Remoue
- Subjects
Liposome ,Bordetella pertussis ,biology ,Chemistry ,Immunogenicity ,Immunology ,Pertactin ,biology.organism_classification ,Microbiology - Published
- 1998
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