24 results on '"Yang, Wu"'
Search Results
2. Fatal multiple organ dysfunction caused by commensal bacteria of urogenital tract infection in adult lung transplant recipients: two case reports
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Tian, Manman, Han, Dongsheng, Ma, Subo, Liu, Tingting, Yang, Wu, and Zheng, Xia
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- 2023
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3. A pangenome analysis pipeline provides insights into functional gene identification in rice
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Wang, Jian, Yang, Wu, Zhang, Shaohong, Hu, Haifei, Yuan, Yuxuan, Dong, Jingfang, Chen, Luo, Ma, Yamei, Yang, Tifeng, Zhou, Lian, Chen, Jiansong, Liu, Bin, Li, Chengdao, Edwards, David, and Zhao, Junliang
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- 2023
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4. FOXO1 reshapes neutrophils to aggravate acute brain damage and promote late depression after traumatic brain injury.
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Zhou, Mi, Liu, Yang-Wu-Yue, He, Yu-Hang, Zhang, Jing-Yu, Guo, Hao, Wang, Hao, Ren, Jia-Kui, Su, Yi-Xun, Yang, Teng, Li, Jia-Bo, He, Wen-Hui, Ma, Peng-Jiao, Mi, Man-Tian, and Dai, Shuang-Shuang
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BRAIN injuries ,BRAIN damage ,FORKHEAD transcription factors ,NEUTROPHILS ,MYELIN basic protein - Abstract
Background: Neutrophils are traditionally viewed as first responders but have a short onset of action in response to traumatic brain injury (TBI). However, the heterogeneity, multifunctionality, and time-dependent modulation of brain damage and outcome mediated by neutrophils after TBI remain poorly understood. Methods: Using the combined single-cell transcriptomics, metabolomics, and proteomics analysis from TBI patients and the TBI mouse model, we investigate a novel neutrophil phenotype and its associated effects on TBI outcome by neurological deficit scoring and behavioral tests. We also characterized the underlying mechanisms both in vitro and in vivo through molecular simulations, signaling detections, gene expression regulation assessments [including dual-luciferase reporter and chromatin immunoprecipitation (ChIP) assays], primary cultures or co-cultures of neutrophils and oligodendrocytes, intracellular iron, and lipid hydroperoxide concentration measurements, as well as forkhead box protein O1 (FOXO1) conditional knockout mice. Results: We identified that high expression of the FOXO1 protein was induced in neutrophils after TBI both in TBI patients and the TBI mouse model. Infiltration of these FOXO1
high neutrophils in the brain was detected not only in the acute phase but also in the chronic phase post-TBI, aggravating acute brain inflammatory damage and promoting late TBI-induced depression. In the acute stage, FOXO1 upregulated cytoplasmic Versican (VCAN) to interact with the apoptosis regulator B-cell lymphoma-2 (BCL-2)-associated X protein (BAX), suppressing the mitochondrial translocation of BAX, which mediated the antiapoptotic effect companied with enhancing interleukin-6 (IL-6) production of FOXO1high neutrophils. In the chronic stage, the "FOXO1-transferrin receptor (TFRC)" mechanism contributes to FOXO1high neutrophil ferroptosis, disturbing the iron homeostasis of oligodendrocytes and inducing a reduction in myelin basic protein, which contributes to the progression of late depression after TBI. Conclusions: FOXO1high neutrophils represent a novel neutrophil phenotype that emerges in response to acute and chronic TBI, which provides insight into the heterogeneity, reprogramming activity, and versatility of neutrophils in TBI. [ABSTRACT FROM AUTHOR]- Published
- 2024
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5. Intrathecal morphine delivery at prepontine cistern to control refractory cancer-related pain: a case report of extensive metastatic and refractory cancer pain.
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Li, Qing, Long, Yan-ling, He, Yun-wu, Long, Hui, Xiao, Zhen-ping, Li, Yong-lin, Yang, Wu-zhou, Jiang, Li-ping, Gao, Wei, and Zou, Cong
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CANCER pain ,SPINAL infusions ,MORPHINE - Abstract
Background: Extensive metastatic and refractory cancer pain is common, and exhibits a dissatisfactory response to the conventional intrathecal infusion of opioid analgesics. Case Presentation: The present study reports a case of an extensive metastatic esophageal cancer patient with severe intractable pain, who underwent translumbar subarachnoid puncture with intrathecal catheterization to the prepontine cistern. After continuous infusion of low-dose morphine, the pain was well-controlled with a decrease in the numeric rating scale (NRS) of pain score from 9 to 0, and the few adverse reactions to the treatment disappeared at a low dose of morphine. Conclusions: The patient achieved a good quality of life during the one-month follow-up period. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Exosomal miR-100-5p inhibits osteogenesis of hBMSCs and angiogenesis of HUVECs by suppressing the BMPR2/Smad1/5/9 signalling pathway
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Yang, Wu, Zhu, Weiwen, Yang, Yunfei, Guo, Minkang, Qian, Husun, Jiang, Weiqian, Chen, Yu, Lian, Chengjie, Xu, Zijie, Bai, Haobo, Chen, Tingmei, and Zhang, Jian
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- 2021
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7. Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11
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Nosheen, Shaista, Naz, Tahira, Yang, Junhuan, Hussain, Syed Ammar, Fazili, Abu Bakr Ahmad, Nazir, Yusuf, Li, Shaoqi, Mohamed, Hassan, Yang, Wu, Mustafa, Kiren, and Song, Yuanda
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- 2021
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8. Epitope-loaded nanoemulsion delivery system with ability of extending antigen release elicits potent Th1 response for intranasal vaccine against Helicobacter pylori
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Yang, Yun, Chen, Li, Sun, Hong-wu, Guo, Hong, Song, Zhen, You, Ying, Yang, Liu-yang, Tong, Ya-nan, Gao, Ji-ning, Zeng, Hao, Yang, Wu-chen, and Zou, Quan-ming
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- 2019
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9. A study on the neurodevelopment outcomes of late preterm infants
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You, Jia, Shamsi, Bilal Haider, Hao, Mei-chen, Cao, Chun-Hong, and Yang, Wu-Yue
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- 2019
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10. Effect of lipid metabolism disorder on liver function in patients with malignant tumors after chemotherapy: a case-control study
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Sun, Yan, Zhang, Nie, Ding, Yun-Long, Yu, Li-Jiang, Cai, Jun, Ma, De, Yang, Wu, Lu, Wang-Kun, and Niu, Jia-Li
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- 2019
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11. Long-term oncologic safety of immediate reconstructive surgery in patients with invasive breast cancer: a retrospective matched-cohort study
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Lijun Hao, Qiqi Wu, Shanshan Sun, Sihang Zhang, Ming Niu, Sai Luo, Jiguang Han, Yang Wu, Jing Xiong, Yanni Song, Dalin Li, Rui Huang, and Haiqian Xu
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Reconstructive surgery ,medicine.medical_specialty ,RD1-811 ,Modified radical mastectomy ,Breast Neoplasms ,Cohort Studies ,Matched cohort ,Breast cancer ,Humans ,Medicine ,Breast conservation surgery ,In patient ,Breast reconstruction ,RC254-282 ,Mastectomy ,Retrospective Studies ,business.industry ,Research ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Patient satisfaction ,Plastic Surgery Procedures ,medicine.disease ,Prognosis ,Term (time) ,Surgery ,Oncology ,Quality of Life ,Female ,business - Abstract
Objective Immediate reconstruction (IR) is a safe and effective surgical treatment for patients with breast cancer. We aimed to assess the prognosis, aesthetic outcomes, and patient satisfaction of IR compared with breast conservation surgery (BCS) and total mastectomy (TM). Methods This retrospective matched-cohort study was conducted between May 2005 and December 2014. We established two cohorts according to the tumor (T) size of breast cancer. In the T≤3cm group, cases (IR) and controls (BCS or TM) were matched for age, pathological tumor size, and pathologic nodal status in a 1:1:1 ratio. In the T>3cm group, cases (IR) and controls (TM) were matched with the same factors and ratio. The primary outcome was the 5-year disease-free survival (DFS). The secondary outcome was patient satisfaction and quality of life. Results A total of 12,678 breast cancer patients were assessed for eligibility, of which 587 were included (T≤3 cm group: 155 IR vs 155 BCS vs 155 TM; T>3cm group: 61 IR vs 61 TM). In the T≤3 cm cohort, patients who underwent IR had no difference compared with those who underwent BCS or TM regarding the 5-year DFS (P=0.539); however, an improved aesthetic satisfaction, psychosocial, and sexual well-being were achieved in the IR group (P3 cm cohort, the IR group had a worse median 5-year DFS (P=0.044), especially for Her2+ or triple-negative breast carcinoma (TNBC) subtypes compared with the TM group. Conclusions IR improves aesthetic satisfaction, psychosocial, and sexual well-being for breast cancer patients with T≤3 cm. For patients with T > 3 cm invasive breast cancer, TM is superior to IR as it predicts a better 5-year DFS.
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- 2021
12. CaLRR-RLK1, a novel RD receptor-like kinase from Capsicum annuum and transcriptionally activated by CaHDZ27, act as positive regulator in Ralstonia solanacearum resistance
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Shuilin He, Sheng Yang, Feng Gao, Lei Shen, Yang Wu, Weihong He, Shaoliang Mou, and Wei Cheng
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0106 biological sciences ,0301 basic medicine ,Hypersensitive response ,Plant Science ,CaHDZ27 ,01 natural sciences ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Plant immunity ,Gene Expression Regulation, Plant ,lcsh:Botany ,Pepper ,Disease Resistance ,Plant Proteins ,CaLRR-RLK1 ,Ralstonia solanacearum ,Methyl jasmonate ,biology ,Kinase ,Bacterial wilt ,food and beverages ,Hydrogen Peroxide ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,lcsh:QK1-989 ,030104 developmental biology ,chemistry ,Capsicum ,Salicylic acid ,010606 plant biology & botany ,Ethephon ,Research Article - Abstract
Background Bacterial wilt caused by Ralstonia solanacearum is one of the most important diseases in pepper worldwide, however, the molecular mechanism underlying pepper resistance to bacterial wilt remains poorly understood. Results Herein, a novel RD leucine-rich repeat receptor-like kinase, CaLRR-RLK1, was functionally characterized in immunity against R. solanacearum. CaLRR-RLK1 was targeted exclusively to plasma membrane and was up-regulated by R. solanacearum inoculation (RSI) as well as by the exogenous application of salicylic acid (SA), methyl jasmonate (MeJA) or ethephon (ETH). The silencing of CaLRR-RLK1 led to enhanced susceptibility of pepper plants to RSI, accompanied by down-regulation of immunity-related genes including CaACO1, CaHIR1, CaPR4 and CaPO2. In contrast, transient overexpression of CaLRR-RLK1 triggered hypersensitive response (HR)-like cell death and H2O2 accumulation in pepper leaves, manifested by darker trypan blue and DAB staining respectively. In addition, the ectopic overexpression of CaLRR-RLK1 in tobacco plants enhanced resistance R. solanacearum, accompanied with the immunity associated marker genes including NtPR2, NtPR2, NtHSR203 and NtHSR515. Furthermore, it was found that CaHDZ27, a positive regulator in pepper response to RSI in our previous study, transcriptionally activated CaLRR-RLK1 by direct targeting its promoter probably in a CAATTATTG dependent manner. Conclusion The study revealed that CaLRR-RLK1 confers pepper resistance to R. solanacearum as the direct targeting of CaHDZ27. Electronic supplementary material The online version of this article (10.1186/s12870-018-1609-6) contains supplementary material, which is available to authorized users.
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- 2019
13. Infant progressive colonic stenosis caused by antibiotic-related Clostridium difficile colitis – a case report and literature review
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Qi Wang, Bo Xiang, Yang Wu, Xiaolong Xie, Yiyang Zhao, and Xiaoping Jiang
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Male ,medicine.medical_specialty ,medicine.medical_treatment ,Case Report ,Constriction, Pathologic ,030230 surgery ,Gastroenterology ,Clostridium Difficile Colitis ,03 medical and health sciences ,Colonic Diseases ,0302 clinical medicine ,030225 pediatrics ,Internal medicine ,Laparotomy ,medicine ,Humans ,Colitis ,Barium enema ,Colonitis ,business.industry ,lcsh:RJ1-570 ,Transverse colon ,Infant, Newborn ,lcsh:Pediatrics ,Clostridium difficile ,Meropenem ,Abdominal distension ,medicine.disease ,digestive system diseases ,Anti-Bacterial Agents ,Radiography ,Stenosis ,Pediatrics, Perinatology and Child Health ,Clostridium Infections ,medicine.symptom ,Colonic stenosis ,business ,Intestinal Obstruction - Abstract
Background Colonic stenosis is a rare cause of pediatric intestinal obstruction. The root cause underlying colonic stenosis is unclear and there is no fixed operation. Case presentation We reported on a male infant with progressive colonic stenosis caused by antibiotic-related colitis. The infant was admitted to our hospital with pneumonia but developed progressive abdominal distension and diarrhea following antibiotic treatment with meropenem. Initial testing of stool culture showed a Clostridium difficile infection. Additional testing with barium enema imaging showed stenosis at the junction of the sigmoid and descending colon at first and another stenosis occurred at the right half of the transverse colon 3 weeks later. Staged surgical treatment was performed with primary resections of the two parts suffering stenosis, ileostomy, and secondary intestinal anastomosis. A pathological exam then confirmed the diagnosis of colonic stenosis and the patient had an uneventful recovery and has been recovering well as evidenced by the 1-year follow-up. Conclusions Based on a review of the literature and our case report, we found that progressive colonic stenosis caused by colitis due to antibiotic-related Clostridium difficile infection is rare in infants. Infants with colitis and repeated abdominal distention, vomiting, and constipation should be treated with the utmost caution and screened. Despite this, clinical manifestations depended on the severity of the stenosis. Barium enema, colonoscopy, laprascopy or laparotomy and colonic biopsy are helpful for diagnosis and differential diagnosis. While both one-stage and multiple-stage operations are feasible, a staged operation should be used for multiple colonic stenoses.
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- 2018
14. Quantifying the mapping precision of genome-wide association studies using whole-genome sequencing data
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Peter M. Visscher, Zhili Zheng, Jian Yang, and Yang Wu
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0301 basic medicine ,Linkage disequilibrium ,lcsh:QH426-470 ,Genotype ,Quantitative Trait Loci ,Genomics ,Genome-wide association study ,Biology ,Genome-wide association studies ,Polymorphism, Single Nucleotide ,Linkage Disequilibrium ,White People ,03 medical and health sciences ,0302 clinical medicine ,Gene Frequency ,Humans ,False positive rate ,lcsh:QH301-705.5 ,Alleles ,Genetic association ,Imputation ,Genome, Human ,Research ,Physical Chromosome Mapping ,High-Throughput Nucleotide Sequencing ,Minor allele frequency ,lcsh:Genetics ,030104 developmental biology ,Phenotype ,lcsh:Biology (General) ,Mapping precision ,Evolutionary biology ,Whole genome sequencing ,Sample Size ,030217 neurology & neurosurgery ,Imputation (genetics) ,Personal genomics ,Genome-Wide Association Study - Abstract
Background Understanding the mapping precision of genome-wide association studies (GWAS), that is the physical distances between the top associated single-nucleotide polymorphisms (SNPs) and the causal variants, is essential to design fine-mapping experiments for complex traits and diseases. Results Using simulations based on whole-genome sequencing (WGS) data from 3642 unrelated individuals of European descent, we show that the association signals at rare causal variants (minor allele frequency ≤ 0.01) are very unlikely to be mapped to common variants in GWAS using either WGS data or imputed data and vice versa. We predict that at least 80% of the common variants identified from published GWAS using imputed data are within 33.5 Kbp of the causal variants, a resolution that is comparable with that using WGS data. Mapping precision at these loci will improve with increasing sample sizes of GWAS in the future. For rare variants, the mapping precision of GWAS using WGS data is extremely high, suggesting WGS is an efficient strategy to detect and fine-map rare variants simultaneously. We further assess the mapping precision by linkage disequilibrium between GWAS hits and causal variants and develop an online tool (gwasMP) to query our results with different thresholds of physical distance and/or linkage disequilibrium (http://cnsgenomics.com/shiny/gwasMP). Conclusions Our findings provide a benchmark to inform future design and development of fine-mapping experiments and technologies to pinpoint the causal variants at GWAS loci. Electronic supplementary material The online version of this article (doi:10.1186/s13059-017-1216-0) contains supplementary material, which is available to authorized users.
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- 2017
15. Long non-coding RNA GAS5 suppresses pancreatic cancer metastasis through modulating miR-32-5p/PTEN axis.
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Zhi-Qiang Gao, Jun-feng Wang, De-Hua Chen, Xue-Song Ma, Yang Wu, Zhe Tang, and Xiao-Wei Dang
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NON-coding RNA ,PANCREATIC cancer ,METASTASIS - Abstract
Background: Long non-coding RNA growth arrest-specific transcript 5 (lncRNA GAS5) is a well-known tumor suppressor in the pathogenesis of a variety of human cancers. The precise role of GAS5 in pancreatic cancer (PC) progression is currently unknown, so the aim of this study was to explore the functional participation of GAS5 in PC metastasis. Methods: The expression changes of GAS5, miR-32-5p and PTEN in human PC specimens and cell lines were compared by means of molecular biology methods. Transfection of the recombinant plasmid was applied to modulate the expression levels of the target genes. RIP and RNA pull-down assays were designed to investigate the interaction between GAS5 and miR-32-5p. The effect of GAS5 and miR-32-5p on PC progression was assessed with cell proliferation, migration, invasion and apoptosis in vitro. Results: GAS5 and PTEN protein were decreased in human PC tissues and cells, but miR-32-5p was increased. GAS5 induction greatly inhibited the proliferation, migration and invasion of PC cells PANC-1 and BxPC-3 in vitro and simultaneously induced cell apoptosis. Moreover, GAS5 positively regulated the expression of PTEN through miR-32-5p. Furthermore, GAS5 suppressed the proliferation, migration and invasion of PC cells through regulating miR-32-5p/PTEN axis. Additionally, this finding was further supported by the results of in vivo experiments. Conclusion: GAS5 could positively regulate PTEN-induced tumor-suppressor pathway via miR-32-5p, thereby suppressing PC metastasis. [ABSTRACT FROM AUTHOR]
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- 2017
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16. Quantifying the mapping precision of genome-wide association studies using whole-genome sequencing data.
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Yang Wu, Zhili Zheng, Visscher, Peter M., and Jian Yang
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- 2017
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17. Reliability analysis of the Chinese version of the Functional Assessment of Cancer Therapy - Leukemia (FACT-Leu) scale based on multivariate generalizability theory.
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Qiong Meng, Zheng Yang, Yang Wu, Yuanyuan Xiao, Xuezhong Gu, Meixia Zhang, Chonghua Wan, Xiaosong Li, Meng, Qiong, Yang, Zheng, Wu, Yang, Xiao, Yuanyuan, Gu, Xuezhong, Zhang, Meixia, Wan, Chonghua, and Li, Xiaosong
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CANCER treatment ,QUALITY of life ,FUNCTIONAL assessment ,MULTIVARIATE analysis ,GENERALIZABILITY theory ,MENTAL health ,LEUKEMIA ,COMPARATIVE studies ,LANGUAGE & languages ,RESEARCH methodology ,MEDICAL cooperation ,PSYCHOMETRICS ,QUESTIONNAIRES ,RESEARCH ,RESEARCH evaluation ,TRANSLATIONS ,ASIANS ,EVALUATION research ,PSYCHOLOGY - Abstract
Background: The Functional Assessment of Cancer Therapy-Leukemia (FACT-Leu) scale, a leukemia-specific instrument for determining the health-related quality of life (HRQOL) in patients with leukemia, had been developed and validated, but there have been no reports on the development of a simplified Chinese version of this scale. This is a new exploration to analyze the reliability of the HRQOL measurement using multivariate generalizability theory (MGT). This study aimed to develop a Chinese version of the FACT-Leu scale and evaluate its reliability using MGT to provide evidence to support the revision and improvement of this scale.Methods: The Chinese version of the FACT-Leu scale was developed by four steps: forward translation, backward translation, cultural adaptation and pilot-testing. The HRQOL was measured for eligible inpatients with leukemia using this scale to provide data. A single-facet multivariate Generalizability Study (G-study) design was demonstrated to estimate the variance-covariance components and then several Decision Studies (D-studies) with varying numbers of items were analyzed to obtain reliability coefficients and to understand how much the measurement reliability could be vary as the number of items in MGT changes.Results: One-hundred and one eligible inpatients diagnosed with leukemia were recruited and completed the HRQOL measurement at the time of admission to the hospital. In the G-study, the variation component of the patient-item interaction was largest while the variation component of the item was the smallest for the four of five domains, except for the leukemia-specific (LEUS) domain. In the D-study, at the level of domain, the generalizability coefficients (G) and the indexes of dependability (Ф) for four of the five domains were approximately equal to or greater than 0.80 except for the Emotional Well-being (EWB) domain (>0.70 but <0.80). For the overall scale, the composite G and composite Ф coefficients were greater than 0.90. Based on the G coefficient and Ф coefficient, two decision options for revising this scale considering the number of items were obtained: one is a 37-item version while the other is a 45-item version.Conclusion: The Chinese version of the FACT-Leu scale has good reliability as a whole based on the results of MGT and the implementation of MGT could lead to more informed decisions in complex questionnaire design and improvement. [ABSTRACT FROM AUTHOR]- Published
- 2017
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18. Associations between matrix metalloproteinase gene polymorphisms and glaucoma susceptibility: a meta-analysis.
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Ming-Yue Wu, Yang Wu, Yong Zhang, Cai-Yun Liu, Chun-Yan Deng, Le Peng, Lan Zhou, Wu, Ming-Yue, Wu, Yang, Zhang, Yong, Liu, Cai-Yun, Deng, Chun-Yan, Peng, Le, and Zhou, Lan
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MATRIX metalloproteinases ,GENETIC polymorphisms ,GLAUCOMA ,META-analysis ,CONFIDENCE intervals - Abstract
Background: Matrix metalloproteinases (MMPs) polymorphisms have been implicated in the pathogenesis of glaucoma risk. However, the results were controversial. We performed a meta-analysis to evaluate the precise associations between MMPs polymorphisms and glaucoma risk.Methods: Related studies were reviewed by searching electronic databases within four databases. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the association between the most common polymorphisms of MMPs and glaucoma risk. Heterogeneity, publication bias and sensitivity analysis were conducted to guarantee the statistical power.Results: Overall, 11 selected articles involving 2,388 cases and 2,319 controls were included in this meta-analysis. Significant associations were only found between MMP-9 rs17576 G > A polymorphism (GA vs. GG: OR = 0.80, 95%CI = 0.67-0.97, P = 0.02, I2 = 0%), MMP-9 rs3918249 C > T polymorphism (TT vs. CC + CT: OR = 0.71, 95%CI = 0.51-0.98, P = 0.04, I2 = 0%) and glaucoma risk in the general population. Subgroup analysis also suggested that MMP-9 rs17576 G > A was related to glaucoma in the Caucasian population (GA vs. GG: OR = 0.67, 95%CI = 0.45-1.00, P = 0.05; GA + AA vs. GG: OR = 0.66, 95%CI = 0.45-0.97, P = 0.03, I2 = 0%).Conclusions: Our meta-analysis demonstrates that MMP-9 rs17576 G > A polymorphism might be a protective factor against the development of glaucoma in Caucasian population. [ABSTRACT FROM AUTHOR]- Published
- 2017
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19. Developmental piRNA profiles of the invasive vector mosquito Aedes albopictus.
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Peiwen Liu, Yunqiao Dong, Jinbao Gu, Santhosh Puthiyakunnon, Yang Wu, and Xiao-Guang Chen
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AEDES albopictus ,AEDES ,MOBILE genetic elements ,DNA insertion elements ,MOLECULAR genetics - Abstract
Background: In eukaryotic organisms, Piwi-interacting RNAs (piRNAs) control the activities of mobile genetic elements and ensure genome maintenance. Recent evidence indicates that piRNAs are involved in multiple biological pathways, including transcriptional regulation of protein-coding genes, sex determination and even interactions between host and pathogens. Aedes albopictus is a major invasive species that transmits a number of viral diseases in humans. Ae. albopictus has the largest genome and the highest abundance of repetitive sequences when compared with members that belong to Culicidae with a published genome. Analysis of piRNA profiles will provide a developmental and evolutionary perspective on piRNAs in Ae. albopictus. Methods: piRNAs were identified and characterized during the development of Ae. albopictus, and piRNA expression patterns in adult males and females as well as sugar-fed females and blood-fed females were compared. Results: Our results reveal that, despite the large genome size of Ae. albopictus, the piRNA pool of Ae. albopictus (1.2 × 107) is smaller than those of Aedes aegypti (1.7 × 107) and Drosophila melanogaster (1.6 × 107). In Ae. albopictus, piRNAs displayed the highest abundance at the embryo stage and the lowest abundance at the pupal stage. Approximately 50 % of the piRNAs mapped to intergenic regions with no known functions. Approximately 30 % of the piRNAs mapped to repetitive elements, and 77.69 % of these repeat-derived piRNAs mapped to Class I TEs; 45.42 % of the observed piRNA reads originated from piRNA clusters, and most of the top 10 highest expressed piRNA clusters and 100 highest expressed piRNAs from each stage displayed biased expression patterns across the developmental stages. All anti-sense-derived piRNAs displayed a preference for uridine at the 5′ end; however, the sense-derived piRNAs showed adenine bias at the tenth nucleotide position and a typical ping-pong signature, suggesting that the biogenesis of piRNAs was conserved throughout development. Our results also show that 962 piRNAs displayed sex-biased expression, and 522 piRNAs showed higher expression in the blood-fed females than in the sugar-fed females. Conclusions: Our results suggest that piRNAs, aside from silencing transposable elements in Ae. albopictus, may have a role in other biological pathways. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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20. Laparoscopic gastrectomy versus open gastrectomy for elderly patients with gastric cancer: a systematic review and meta-analysis.
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Jin-fa Wang, Song-ze Zhang, Neng-yun Zhang, Zong-yang Wu, Ji-ye Feng, Li-ping Ying, and Jing-jing Zhang
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GASTRECTOMY ,LAPAROSCOPIC surgery ,FEASIBILITY studies ,META-analysis ,BLOOD loss estimation ,LYMPH node diseases ,THERAPEUTICS - Abstract
Background: The objective of this study was to evaluate the feasibility, safety, and potential benefits of laparoscopic gastrectomy (LG) comparing with open gastrectomy (OG) in elderly population. Methods: Studies comparing LG with OG for elderly population with gastric cancer, published between January 1994 and July 2015, were identified in the PubMed, Embase, and ISI Web of Science databases. Operative outcomes (intraoperative blood loss, operative time, and the number of lymph nodes harvested) and postoperative outcomes (time to first ambulation, time to first flatus, time to first oral intake, postoperative hospital stay, postoperative morbidity) were included and analyzed. The Newcastle-Ottawa Scale was used to assess the quality of the pooled study. A funnel plot was used to evaluate the publication bias. Results: Seven studies totaling 845 patients were included in the meta-analysis. LG in comparison to OG showed less intraoperative blood loss (weighted mean difference (WMD) -127.47; 95 % confidence interval (CI) -202.79 to -52.16; P < 0.01), earlier time to first ambulation (WMD -2.07; 95 % CI -2.84 to -1.30; P < 0.01), first flatus (WMD -1.04; 95 % CI -1.45 to -0.63; P < 0.01), and oral intake (WMD -0.94; 95 % CI -1.11 to -0.77; P < 0.01), postoperative hospital stay (WMD -5.26; 95 % CI -7.58 to -2.93; P < 0.01), lower overall postoperative complication rate (odd ratio (OR) 0.39; 95 % CI 0.28 to 0.55; P < 0.01), less surgical complications (OR 0.47; 95 % CI 0.32 to 0.69; P < 0.01), medical complication (OR 0.35; 95 % CI 0.22 to 0.56; P < 0.01), incisional complication (OR 0.40; 95 % CI 0.19 to 0.85; P = 0.02), and pulmonary infection (OR 0.49; 95 % CI 0.26 to 0.93; P = 0.03). No significant differences were observed between LG and OG for the number of harvested lymph nodes. However, LG had longer operative times (WMD 15.73; 95 % CI 6.23 to 25.23; P < 0.01). Conclusions: LG is a feasible and safe approach for elderly patients with gastric cancer. Compared with OG, LG has less blood loss, faster postoperative recovery, and reduced postoperative morbidity. [ABSTRACT FROM AUTHOR]
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- 2016
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21. An external sensing system in Plasmodium falciparum-infected erythrocytes.
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Yang Wu, Cruz, Laura N., Szestak, Tadge, Laing, Gavin, Molyneux, Gemma R., Garcia, Celia R. S., and Craig, Alister G.
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PLASMODIUM falciparum , *ERYTHROCYTES , *CELLULAR signal transduction , *GEL electrophoresis , *GENE expression , *CYTOPLASM - Abstract
Background: A number of experiments have previously indicated that Plasmodium falciparum-infected erythrocytes (pRBC) were able to sense host environment. The basis of this ability to detect external cues is not known but in screening signalling molecules from pRBC using commercial antibodies, a 34 kDa phosphorylated molecule that possesses such ability was identified. Methods: The pRBC were exposed to different culture conditions and proteins were extracted for 1D or 2D gel electrophoresis followed by Western blot. The localization of 34 kDa protein was examined by biochemical fractionation followed by Western blot. High-resolution mass spectrometric analysis of immune precipitants was used to identify this protein and real-time quantitative reverse transcriptase polymerase chain reaction was used for detecting mRNA expression level. Results: The 34 kDa protein was called PfAB4 has immediate responses (dephosphorylation and rapid turnover) to host environmental stimuli such as serum depletion, osmolality change and cytokine addition. PfAB4 is expressed constitutively throughout the erythrocytic lifecycle with dominant expression in trophozoites 30 h post-infection. Tumour necrosis factor (TNF) treatment induced a transient detectable dephosphorylation of PfAB4 in the ItG strain (2 min after addition) and the level of expression and phosphorylation returned to normal within 1-2 h. PfAB4 localized dominantly in pRBC cytoplasm, with a transient shift to the nucleus under TNF stimulation as shown by biochemical fractionation. High-resolution mass spectrometric analysis of immune precipitants of AB4 antibodies revealed a 34 kDa PfAB4 component as a mixture of proliferating cellular nuclear antigen-1 (PCNA1) and exported protein-2 (EXP2), along with a small number of other inconsistently identified peptides. Different parasite strains have different PfAB4 expression levels, but no significant association between mRNA and PfAB4 levels was seen, indicating that the differences may be at the post-transcriptional, presumably phosphorylation, level. A triple serine phosphorylated PCNA1 peptide was identified from the PfAB4 high expression strain only, providing further evidence that the identity of PfAB4 is PCNA1 in P. falciparum. Conclusion: A protein element in the human malaria parasite that responds to external cues, including the proinflammatory cytokine TNF have been discovered. Treatment results in a transient change in phosphorylation status of the response element, which also migrates from the parasite cytoplasm to the nucleus. The response element has been identified as PfPCNA1. This sensing response could be regulated by a parasite checkpoint system and be analogous to bacterial two-component signal transduction systems. [ABSTRACT FROM AUTHOR]
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- 2016
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22. Identified single-nucleotide polymorphisms and haplotypes at 16q22.1 increase diabetic nephropathy risk in Han Chinese population.
- Author
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Li-Na Liao, Ching-Chu Chen, Fang-Yang Wu, Cheng-Chieh Lin, Jen-Hao Hsiao, Chwen-Tzuei Chang, Kardia, Sharon L. R., Tsai-Chung Li, and Fuu-Jen Tsai
- Subjects
HAPLOTYPES ,CHRONIC kidney failure ,GENETIC polymorphisms ,DIABETIC nephropathies ,PEOPLE with diabetes ,DISEASE risk factors - Abstract
Background Diabetic nephropathy (DN) has become one of the most common causes of end-stage renal disease (ESRD) in many countries, such as 44.5% in Taiwan. Previous studies have shown that there is a genetic component to ESRD. Studies attempting to determine which genetic variants are related to DN in Han Chinese are limited. Results We included 574 unrelated type 2 diabetes patients (217 DN cases and 357 controls), who were genotyped using Illumina HumanHap550-Duo BeadChip. In single-SNP association tests, the SNPs rs11647932, rs11645214, and rs6499323 located at 16q22.1 under the additive-effect disease model were significantly associated with an approximately 2-fold increased risk of DN. In haplotype association tests, identified haplotypes located in the chromosome 16q22.1 region (containing ST3GAL2, COG4, SF3B3, and IL34 genes) raised DN risk. The strongest association was found with haplotype rs2288491-rs4985534- rs11645214 (C-C-G) (adjusted odds ratio [AOR] 1.93, 95% confidence interval [CI] 1.83- 2.03, p =6.25 × 10
-7 ), followed by haplotype rs8052125-rs2288491-rs4985534-rs11645214 (G-C-C-G) (AOR 1.92, 95% CI 1.82-2.02, p =6.56 × 10-7 ), and haplotype rs2303792- rs8052125-rs2288491-rs4985534-rs11645214 (A-G-C-C-G) (AOR 1.91, 95% CI 1.81-2.01, p =1.15 × 10-6 ). Conclusions Our results demonstrate that the novel SNPs and haplotypes located at the 16q22.1 region may involve in the biological pathways of DN in Han Chinese patients with type 2 diabetes. This study can provide new insights into the etiology of DN. [ABSTRACT FROM AUTHOR]- Published
- 2014
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23. Biological activities of fusarochromanone: a potent anti-cancer agent.
- Author
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Mahdavian, Elahe, Palyok, Phillip, Adelmund, Steven, Williams-Hart, Tara, Furmanski, Brian D., Yoon-Jee Kim, Ying Gu, Barzegar, Mansoureh, Yang Wu, Bhinge, Kaustubh N., Kolluru, Gopi K., Quick, Quincy, Yong-Yu Liu, Kevil, Christopher G., Salvatore, Brian A., Shile Huang, and Clifford, John L.
- Subjects
ANTINEOPLASTIC agents ,FUNGAL metabolites ,CELL cycle ,ENDOTHELIAL growth factors ,SMALL molecules ,APOPTOSIS ,CELLULAR signal transduction - Abstract
Background: Fusarochromanone (FC101) is a small molecule fungal metabolite with a host of interesting biological functions, including very potent anti-angiogenic and direct anti-cancer activity. Results: Herein, we report that FC101 exhibits very potent in-vitro growth inhibitory effects (IC
50 ranging from 10nM-2.5 μM) against HaCat (pre-malignant skin), P9-WT (malignant skin), MCF-7 (low malignant breast), MDA-231 (malignant breast), SV-HUC (premalignant bladder), UM-UC14 (malignant bladder), and PC3 (malignant prostate) in a time-course and dose-dependent manner, with the UM-UC14 cells being the most sensitive. FC101 induces apoptosis and an increase in proportion of cells in the sub-G1 phase in both HaCat and P9-WT cell lines as evidenced by cell cycle profile analysis. In a mouse xenograft SCC tumor model, FC101 was well tolerated, non-toxic, and achieved a 30% reduction in tumor size at a dose of 8 mg/kg/day. FC101 is also a potent anti-angiogenenic agent. At nanomolar doses, FC101 inhibits the vascular endothelial growth factor-A (VEGF-A)-mediated proliferation of endothelial cells. Conclusions: Our data presented here indicates that FC101 is an excellent lead candidate for a small molecule anti-cancer agent that simultaneously affects angiogenesis signaling, cancer signal transduction, and apoptosis. Further understanding of the underlying FC101's molecular mechanism may lead to the design of novel targeted and selective therapeutics, both of which are pursued targets in cancer drug discovery. [ABSTRACT FROM AUTHOR]- Published
- 2014
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24. The impact of dialysis therapy on older patients with advanced chronic kidney disease: a nationwide population-based study.
- Author
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Shih CJ, Chen YT, Ou SM, Yang WC, Kuo SC, and Tarng DC
- Subjects
- Aged, Aged, 80 and over, Cohort Studies, Cost-Benefit Analysis, Databases, Factual, Female, Health Care Costs, Humans, Male, National Health Programs, Renal Dialysis economics, Renal Dialysis statistics & numerical data, Renal Insufficiency, Chronic economics, Renal Insufficiency, Chronic mortality, Retrospective Studies, Risk Factors, Taiwan, Decision Making, Renal Insufficiency, Chronic therapy
- Abstract
Background: Older patients with advanced chronic kidney disease (CKD) face the decision of whether to undergo dialysis. Currently available data on this issue are limited because they were generated by small, short-term studies with statistical drawbacks. Further research is urgently needed to provide objective information for dialysis decision making in older patients with advanced CKD., Methods: This nationwide population-based cohort study was conducted using Taiwan's National Health Insurance Research Database. Data from 2000 to 2010 were extracted. A total of 8,341 patients≥70 years old with advanced CKD and serum creatinine levels>6 mg/dl, who had been treated with erythropoiesis-stimulating agents were included. Cox proportional hazard models in which initiation of chronic dialysis was defined as the time-dependent covariate were used to calculate adjusted hazard ratios for mortality. The endpoint was all-cause mortality., Results: During a median follow-up period of 2.7 years, 6,292 (75.4%) older patients chose dialysis therapy and 2,049 (24.6%) received conservative care. Dialysis was initiated to treat kidney failure a median of 6.4 months after enrollment. Dialysis was associated with a 1.4-fold increased risk of mortality compared with conservative care (adjusted hazard ratio 1.39, 95% confidence interval 1.30 to 1.49). In subgroup analyses, the risk of mortality remained consistently increased, independent of age, sex and comorbidities., Conclusions: In older patients, dialysis may be associated with increased mortality risk and healthcare cost compared with conservative care. For patients who are ≥70 years old with advanced CKD, decision making about whether to undergo dialysis should be weighted by consideration of risks and benefits.
- Published
- 2014
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