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Objective: The ultra-small superparamagnetic iron oxide particles were used as the carrier to construct and characterize nuclear magnetic/fluorescent molecular probes through polyethylene glycol-linked fluorescent Cy5.5. Methods: Ake a small amount of Cy5.5-nhs fluorescent powder and dissolve it in dimethyl sulfoxide （DMSO） solution. After centrifugation and ultrafiltration of PEG ferric oxide particles, resuspend the nanoparticles with phosphate buffered saline （PBS） to change the pH of PEG ferric oxide nanoparticle solution. The configured cy5.5 fluorescence was added to Fe3O4particles, incubated in a constant temperature shaking table, and the larger iron ions and unbound fluorescence were removed through a centrifugal filter. After standing, the hydrated particle size and zeta potential were detected, the relaxation rate was detected by newwheat nuclear magnetic resonance, the cytotoxicity was detected by CCK-8 experiment, and the uptake of the probe by cells was observed by laser confocal microscope. Results: The Cy5.5-PEG-Fe3O4probe was successfully synthesized. Transmission electron microscope （TEM） showed that the diameter of the probe was 16.8 ±2.4nm,the water and diameter of the nanoparticles were 43.4±17.6 nm, and the Zeta potential was -18.0 m V. The relaxation rate is 39.5 m M-1·s-1and the slope is 0.98. The results of cytotoxicity experiments show that it is slightly toxic to cells, and the toxicity is dependent on the concentration. Laser confocal results show that this probe can be taken up by cells smoothly. Conclusion: This experiment successfully synthesized Cy5.5-PEG-Fe3O4probe. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of RNA interference CMTM7 on apoptosis of lung adenocarcinoma A549 cell by the. Methods: Selected lung adenocarcinoma A549 cell were divided into si RNA group, negative control group and blank control group,and the RNA interference CMTM7 vectors were transfected into the logarithmic growth of A549 cells, observed A549 cell growth, and the apoptosis and cell cycle status was detected. Results: MTT experiment showed that the inhibition rate in si RNA transfection group was significantly higher than that of the negative control group and blank control group(P>0.05), while the difference between the negative control group and the control group were not significant(P<0.05). Flow cytometry showed the apoptosis rate in the si RNA transfection group was significantly higher than that of the negative control group and blank control group(P<0.05), while the difference between the negative control group and the control group were not significant(P>0.05). Flow cytometry experiments showed that the number of G0 / G1 phase cell in the si RNA transfection group was increased with significantly difference compared with blank control group and negative control group(P<0.05), while the number of G2 / M phase cell in the si RNA transfection group was less than that of the control group and negative control group(P>0.05), but it had no significant difference between the blank control group and negative control group(P>0.05). Conclusions: RNA interference CMTM7 can promote lung adenocarcinoma A549 cell apoptosis, inhibit the growth of tumor cells, and its mechanism may be achieved by interfering with the cell cycle. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of sevoflurane combined with remifentanil on neurologic function, cognitive function and inflammatory factors in patients with intracranial aneurysms undergoing interventional therapy. Methods: 80 patients with intracranial aneurysms who underwent interventional surgery in our hospital from January 2020 to June 2022 were selected and divided into group A(sevoflurane combined with remifentanil) and group B(propofol combined with remifentanil) according to different anesthesia methods, with 40 patients in each group. Neurological function, cognitive function and inflammatory factor level were compared between the two groups. Results: The heart rate(HR) and mean arterial pressure(MAP) of group A were better than those of group B from T1 to T3(P＜0.05). The national institutes of health stroke scale(NIHSS) score of group A was significantly lower than that of group B at 24 h and 7 d after operation(P＜0.05), and the mini-mental state examination(MMSE) score was higher than that of group B(P＜0.05).Serum tumor necrosis factor-α(TNF-α), C-reactive protein(CRP), and interleukin-6(IL-6) levels at 24 h and 3 d after operation in group A were lower than those in group B(P＜0.05). The recovery time of spontaneous respiration, recovery time, extubation time and the incidence of agitation after extubation in group A were lower than those in group B(P＜0.05). The adverse effects were not different(P＞0.05).Conclusion: The application of sevoflurane combined with remifentanil maintenance anesthesia in interventional IA patients can smooth hemodynamics, improve postoperative neurological function and cognitive function, reduce inflammatory response, and improve the quality of anesthesia awakening. [ABSTRACT FROM AUTHOR]

Objective: To investigate the changes of plasma endothelin-1 (ET-1) and NO before and after balloon angioplasty in patients with atherosclerotic occlusive disease (ASO) below the knee and its clinical significance. Method: A total of 38 patients with ASO below the knee, who underwent balloon angioplasty in department of vascular surgery of Tsitsihar First Hospital from February 2013 to February 2014, were selected. The changes of plasma ET-1 and NO levels before operation and 6 hours, 24 hours, 1 week, 1 month and 3 months after operation were compared. All the patients were checked with CT angiography(CTA) 1 month and 3 months after operation respectively to observed whether vascular restenosis occurred or not, and the relationship between vascular restenosis and plasma ET-1 and NO levels was analyzed. Results: Compared with before operation, the plasma ET-1 levels 6 hours after operation increased significantly and decreased slightly from 24 hours to 1 week after operation in all the patients, but still kept at a high level, while the changes of NO was opposite to plasma ET-1.There were 33 patients without vascular restenosis after checked with CTA, the plasma ET-1 and NO levels resumed to preoperative level 1 month after operation and kept till 3 months after operation; however, there were 5 patients with vascular restenosis, the plasma ET-1 level kept at high level from 1 month to 3 months after operation, and the changes of NO was opposite to plasma ET-1 level. Conclusion: The plasma ET-1 and NO levels was related with vascular restenosis after balloon angioplasty in patients with ASO below the knee. [ABSTRACT FROM AUTHOR]

Objective: To analyze the reason of upgrade in Gleason score between initial prostate biopsy and final radical prostatectomy specimens and develop a nomogram to predict the probability of pathological upgrade.Methods: From August 2008 to December 2013, a total of 264 prostate cancer patients underwent RP in our department were selected and divided into two groups according to the upgrade of biopsy and final pathology GS. Both a univariate analysis and a Forward conditional multivariate logistic regression model (LRM) analysis were performed to identify the risk factor (s) of pathological upgrade. A nomogram predicting the probability of pathological upgrade was established by using LRM regression coefficients. Results: In total, 238 of the 264 patients were analyzed. Multivariate logistic regression analysis showed that higher prostate specific antigen density (PSAD) (0R=3.854, P=0.001) and low grade (≤ 6) biopsy GS were. The dependent risk factors of the pathological upgrade after radical prostatectomy. The optimal cutoff value of PASD and biopsy score were 0.37 ng/ml 2 and 8 points. A nomogram combining aforementioned two variables to predict pathological upgrading was established.Conclusion: PASD and biopsy Gleason score were independent risk factors for predicting the postoperative pathological upgrade in Chinese population, the nomogram established in the study could well predict the pathological upgrade. [ABSTRACT FROM AUTHOR]

To observe the efficacy of pelvic floor muscle rehabilitation training combined with pelvic floor reconstruction in the treatment of female pelvic floor dysfunction (PFD) diseases and its effect on inflammatory response and stress response. 200 patients with PFD who were treated in our hospital from January 2019 to January 2021 were selected, and they were randomly divided into control group (100 cases) and study group (100 cases) by random number table method. Patients in the control group received pelvic floor reconstruction, and patients in the study group received pelvic floor muscle rehabilitation training combined with pelvic floor reconstruction. The efficacy and postoperative complications of the two groups were observed, and the related scale scores, inflammatory response and stress response indexes of the two groups were compared. After treatment, the scores of pelvic floor impact questionnaire short form (PFIQ-7), pelvic floor distress inventory short form (PFDI-20) in the study group were lower than those in the control group (P<0.05). The pelvic floor class I muscle fiber voltage and pelvic floor class II muscle fiber voltage in the study group were higher than those in the control group after treatment (P<0.05). Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) and high sensitivity C-reactive protein (hs-CRP) in the study group after treatment were lower than those in the control group (P<0.05). Cortisol (COR), norepinephrine (NE) and prostaglandin E2 (PGE2) in the study group after treatment were lower than those in the control group (P<0.05). The incidence of postoperative complications in the study group was lower than that in the control group (P<0.05). Pelvic floor muscle rehabilitation training combined with pelvic floor reconstruction in the treatment of PFD can promote the recovery of pelvic floor function, reduce inflammatory reaction and stress reaction, and reduce the incidence of postoperative complications. [ABSTRACT FROM AUTHOR]

Objective: To study the clinical effect of free peroneal artery perforator flap on the small wound of hand infection and its effects on the serum inflammatory factors. Methods: 90 patients with small wound of hand who were treated in our hospital from October 2014 to October 2016 were selected and divided into the observation group (n=45) and the control group (n=45). according to the random number table. The control group was treated with inferior epigastric artery perforator flap repair, while the observation group was treated with free peroneal artery perforator flap. Then the operative effect and serum interleukin (IL)-6, IL -8, interferon (INF)-7, tumor necrosis factor (TNF)-α, procalcitonin(PCR), high sensitive C reactive protein(hs-CRP) levels before and after treatment as well as the incidence of infection were compared between two groups. Results: Compared with those before operation, the levels of serum IL-6, IL-8, INF-γ，TNF-α, procalcitonin(PCR), high sensitive C reactive protein(hs-CRP), erythrocyte sedimentation rate (ESR) of both groups were significantly decreased, which were obviously lower in the observation group than those of the control group(P< 0.05); the excellent and good rate after operation in the observation group was higher than that of the control group (P< 0.05); the infection rate of the observation group was lower than that of the control group (P< 0.05). Conclusion: Free peroneal artery perforator flap was effective and safe for small wound of hand, which could significantly inhibit the inflammatory response and desease the postoperative infection. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of serum prolactin, IGF-1 and clinical efficacy by temozolomide in patients with hypophysoma. Methods: A total of 70 patients with hypophysoma fromour hospital were collected and randomly divided into experiment group and control group with 35 cases in each group. Patients in the control group were treated by paranasal sinus surgical treatment with microscope, and bromocriptine 1.25 mg/time oral, 3 times/day, patients in the experiment group were treated on the base of the control group with temozolomide. After treatment, compared the serum prolactin, IGF-1 and GH levels between the two groups before and after treatment. Results: Compared with before treatment, the PRL, IGF-1 and GH levels were decreased after treatment (P<0.05), TSH was decreased, FT4 and ACTH were increased, the difference was statistical significance (P<0.05); compared with the control group, the total effective rate of the experiment group was higher, the PRL and IGF-1 levels were lower (P<0.05), TSH level was lower, FT4 and ACTH levels were higher, the difference was statistical significance (P<0.05). Conclusions: Temozolomide in the treatment of hypophysoma has a good clinical effect, speculate that the mechanismis relate to the decrease of serumprolactin and IGF-1 levels. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of oxiracetam combined with nimodipine on the serum IGF-1. sICAM-1 and sVCAM-1 levels of patients with spontaneous subarachnoid hemorrhage. Methods: 46 patients with spontaneous subarachnoid hemorrhage admitted in our hospital were selected and randomly divided into the control group and the experimental group with 23 cases in each group. The control group was treated by Nimodipine and the experimental group was treated on the basis of the control group with Oxiracetam. The serum IGF-1. sICAM-1 and sVCAM-1 levels were detected and compared between two group before and after treatment. The total clinical efficiency was compared between two groups after treatment. Results: Compared with before treatment, the serum IGF-1 levels of both groups were higher (P<0.05). the sIC'AM-l and sVCAM-1 levels of both groups were lower (P<0.05). Compared with the control group after treatment, the serum IGF-1 level of experimental group was higher (P< 0.05). the sICAM-1 and sVCAM-1 levels were lower (P<0.05), the total clinical efficiency was higher (P<0.05). Conclusion: Oxiracetam combined with nimodipine could improve the curative effect of patients with spontaneous subarachnoid hemorrhage, the mechanism might be related to the increase of serum IGF-1 level and decrease of serum sICAM-1 and sVCAM-1 levels. [ABSTRACT FROM AUTHOR]