1. Toxicity of Tributyltin in Juvenile Common Carp (Cyprinus Carpio): Physiological Responses, Hepatic Gene Expression, and Stress Protein Profiling
- Author
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Liqiao Zhong, Zhi-Hua Li, Yan-Hua Wu, and Wei-Na Mu
- Subjects
Antioxidant ,Health, Toxicology and Mutagenesis ,medicine.medical_treatment ,ATPase ,0211 other engineering and technologies ,02 engineering and technology ,010501 environmental sciences ,Biology ,Toxicology ,medicine.disease_cause ,Bioinformatics ,01 natural sciences ,Biochemistry ,Cyprinus ,Common carp ,chemistry.chemical_compound ,Gene expression ,medicine ,Molecular Biology ,0105 earth and related environmental sciences ,021110 strategic, defence & security studies ,General Medicine ,biology.organism_classification ,Molecular biology ,chemistry ,Toxicity ,biology.protein ,Tributyltin ,Molecular Medicine ,Oxidative stress - Abstract
In this study, the effects of tributyltin (TBT) on biochemical parameters (antioxidant responses and Na(+) -K(+) -ATPase) in different tissues were investigated by using juvenile common carp (Cyprinus Carpio) as well as growth and ion regulation-related genes expression and stress-related proteins profiling in fish liver. Oxidative stress indices and Na(+) -K(+) -ATPase showed tissues-specific responses in fish exposed to different TBT concentrations. All tested genes related to GH/IGF-I axis and ion-regulation were significantly induced in the TBT group with lower concentrations (except for the igfbp3 in 10 μg/L) and were inhibited in 20 μg/L. In addition, the profiling of two proteins Hsp 70 and MT were increasing in a dose-dependent manner under TBT stress. In short, TBT-induced biochemical and molecular responses in different tissues were reflected in the measured parameters in the test. On the basis of TBT residue levels in the natural environment, more long-term experiments at lower concentrations will be necessary in the future.
- Published
- 2015