1. ABCC2 is a functional receptor of Bacillus thuringiensis Cry1Ca in Spodoptera litura
- Author
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Yunpeng Duan, Xue Yao, Gemei Liang, Chen Liu, Shiheng An, and Jizhen Wei
- Subjects
Protein subunit ,Bacillus thuringiensis ,Spodoptera litura ,ATP-binding cassette transporter ,Sf9 ,Spodoptera ,Biochemistry ,Structural Biology ,Protein Interaction Mapping ,Sf9 Cells ,Animals ,Molecular Biology ,Bacillus thuringiensis Toxins ,biology ,Chemistry ,fungi ,General Medicine ,Ligand (biochemistry) ,biology.organism_classification ,Multidrug Resistance-Associated Protein 2 ,In vitro ,Blot ,Host-Pathogen Interactions ,Protein Binding - Abstract
Spodoptera litura is a serious polyphagous pest in the whole world, which has developed resistance to most conventional insecticides and even some Bacillus thuringiensis (Bt) toxins. Cry1Ca has excellent insecticide activity against S. litura with potential application to control S. litura and delay the development of insect resistance. However, the mode of action of Cry1Ca in S. litura is poorly understood. Here, Cry1Ca-binding proteins were identified from S. litura by using pull down assays and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results indicated that aminopeptidase-N (APN), ATP binding cassette subfamily C member 2 (ABCC2), polycalin, actin and V-type proton ATPase subunit A may bind with Cry1Ca. Further study confirmed that ABCC2 fragment expressed in vitro can bind to Cry1Ca as demonstrated by Ligand blot and homologous competition experiments. The over-expression of endogenous SlABCC2 in Sf9 cells increased Cry1Ca cytotoxicity. Correspondingly, the vivo loss of function analyses by SlABCC2 small interfering RNAs (siRNAs) in S. litura larvae decreased the toxicity of Cry1Ca to larvae. Altogether, these results show that ABCC2 of S. litura is a functional receptor that is involved in the action mode of Cry1Ca.
- Published
- 2022