11 results on '"Radhouani, H."'
Search Results
2. Molecular characterization of extended-spectrum-beta-lactamase-producing Escherichia coli isolates from red foxes in Portugal.
- Author
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Radhouani H, Igrejas G, Gonçalves A, Estepa V, Sargo R, Torres C, and Poeta P
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Bacterial Typing Techniques, Cephalosporins pharmacology, Electrophoresis, Gel, Pulsed-Field, Escherichia coli enzymology, Escherichia coli genetics, Escherichia coli isolation & purification, Feces microbiology, Integrons, Microbial Sensitivity Tests, Multilocus Sequence Typing, Phylogeny, Portugal, Promoter Regions, Genetic, Cephalosporin Resistance, Escherichia coli classification, Foxes microbiology, beta-Lactamases genetics
- Abstract
The presence of broad-spectrum-cephalosporin-resistant Escherichia coli isolates and the implicated mechanisms of resistance and virulence factor genes were investigated in red fox (Vulpes vulpes) in Portugal. Cefotaxime-resistant E. coli isolates were isolated from two of 52 fecal samples (4 %), being both ESBL producers. The β-lactamase genes found in the two isolates were bla(SHV-12) + bla(TEM-1b). The tet(A) and sul2 genes were also detected in these isolates, together with the non-classical class 1 integron (intI1-dfrA12-orfF-aadA2-cmlA1-aadA1-qacH-IS440-sul3) with the PcH1 promoter. The two isolates belonged to the phylogroup A. Amino acid changes in GyrA (S83L + D87G) and ParC (S80I) proteins were identified in our study. Concerning MLST typing, both isolates were assigned to ST1086, never found before in wild animals, and they presented closely related PFGE patterns. This study reveals the presence of ESBL-producing E. coli isolates, in a wild ecosystem, which could be disseminated through the environment to other niches.
- Published
- 2013
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3. High prevalence of ESBL-producing Escherichia coli isolates among hemodialysis patients in Portugal: appearance of ST410 with the bla(CTX-M-14) gene.
- Author
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Correia S, Pacheco R, Radhouani H, Diniz JC, Ponce P, Jones-Dias D, Caniça M, Igrejas G, and Poeta P
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- DNA, Bacterial genetics, Escherichia coli drug effects, Escherichia coli genetics, Feces microbiology, Humans, Microbial Sensitivity Tests, Portugal epidemiology, Prevalence, Virulence Factors genetics, beta-Lactam Resistance, beta-Lactamases genetics, Escherichia coli enzymology, Escherichia coli isolation & purification, Escherichia coli Infections epidemiology, Escherichia coli Infections microbiology, Renal Dialysis, beta-Lactamases metabolism
- Abstract
Ten extended-spectrum β-lactamase-producing Escherichia coli isolates were detected among 121 fecal samples (8.3%) recovered from hemodialysis patients in Portugal. The isolates harbored the bla(CTX-M-15), bla(CTX-M-14a), and/or bla(CTX-M-1) genes. A new sequence type, ST2229, was detected, and this study also reports, for the first time, ST410 CTX-M-14-producing isolates., (Copyright © 2012 Elsevier Inc. All rights reserved.)
- Published
- 2012
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4. Iberian wolf as a reservoir of extended-spectrum β-lactamase-producing Escherichia coli of the TEM, SHV, and CTX-M groups.
- Author
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Gonçalves A, Igrejas G, Radhouani H, Estepa V, Pacheco R, Monteiro R, Brito F, Guerra A, Petrucci-Fonseca F, Torres C, and Poeta P
- Subjects
- Animals, DNA, Bacterial genetics, Drug Resistance, Bacterial genetics, Electrophoresis, Gel, Pulsed-Field, Escherichia coli genetics, Escherichia coli isolation & purification, Escherichia coli Infections microbiology, Escherichia coli Proteins genetics, Escherichia coli Proteins metabolism, Feces microbiology, Integrons, Microbial Sensitivity Tests, Phylogeny, Portugal, Virulence Factors genetics, beta-Lactamases classification, beta-Lactamases genetics, Anti-Bacterial Agents pharmacology, Escherichia coli drug effects, Escherichia coli enzymology, Escherichia coli Infections veterinary, Wolves microbiology, beta-Lactamases metabolism
- Abstract
The intensive use of antibiotics in human and veterinary medicine, associated with mechanisms of bacterial genetic transfer, caused a selective pressure that contributed to the dissemination of antimicrobial resistance in different bacteria groups and throughout different ecosystems. Iberian wolf, due to his predatory and wild nature, may serve as an important indicator of environmental contamination with antimicrobial resistant bacteria. The aim of this study was to characterize the diversity of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates within the fecal microbiota of Iberian wolf. Additionally, the identification of other associated resistance genes, phylogenetic groups, and the detection of virulence determinants were also focused on in this study. From 2008 to 2009, 237 fecal samples from Iberian wolf were collected in Portugal. E. coli isolates with TEM-52, SHV-12, CTX-M-1, and CTX-M-14-type ESBLs were detected in 13 of these samples (5.5%). This study reveals the presence of ESBL-producing E. coli isolates, in a wild ecosystem, which could be disseminated through the environment. Moreover, the presence of resistant genes in integrons and the existence of virulence determinants were shown. The association between antibiotic resistance and virulence determinants should be monitored, as it constitutes a serious public health problem.
- Published
- 2012
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5. Genetic characterisation of extended-spectrum β-lactamases in Escherichia coli isolated from retail chicken products including CTX-M-9 containing isolates: a food safety risk factor.
- Author
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Silva N, Costa L, Gonçalves A, Sousa M, Radhouani H, Brito F, Igrejas G, and Poeta P
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- Animals, Chickens, Escherichia coli classification, Escherichia coli drug effects, Escherichia coli enzymology, Escherichia coli Infections epidemiology, Escherichia coli Infections microbiology, Escherichia coli Proteins metabolism, Polymerase Chain Reaction veterinary, Portugal epidemiology, Poultry Diseases epidemiology, Risk Factors, Sequence Analysis, DNA veterinary, beta-Lactamases metabolism, Escherichia coli genetics, Escherichia coli Infections veterinary, Escherichia coli Proteins genetics, Meat microbiology, Poultry Diseases microbiology, beta-Lactam Resistance, beta-Lactamases genetics, beta-Lactams pharmacology
- Abstract
1. Bacterial resistance to β-lactam antibiotics has risen dramatically in Escherichia coli from food animals. In a previous study, 29 randomly selected chicken products, collected in Portugal, were analysed for the presence of extended-spectrum β-lactamases (ESBLs)-producing E. coli; and during this study the genetic characterisation of ESBLs genes was investigated. 2. The presence of genes encoding TEM, OXA, SHV, and CTX-M type beta-lactamases was studied by PCR followed by sequencing. Additionally, other mechanisms of antimicrobial resistance, phylogenetic groups and the presence of virulence determinants were evaluated among the isolates. 3. β-lactamases genes were identified as follows: bla (CTX-M-14) (n = 4), bla (CTX-M-1) (n = 2), bla (CTX-M-9) (n = 4) and bla (TEM-52) (n = 13). Mutations at positions -42, -18, -1, and +58 of ampC promoter region were identified in 4 non-ESBL-producing isolates. The tet(A) or tet(B) genes were identified in all tetracycline-resistant isolates; the aadA gene detected in 8 of 10 streptomycin-resistant isolates; the aac(3)-II gene in all gentamicin-resistant isolates; the cmlA gene in the chloramphenicol-resistant isolate; and sul1 and/or sul2 and/or sul3 genes were found in all trimethoprim-sulfamethoxazole-resistant isolates. The intI1 gene was detected in 8 trimethoprim-sulfamethoxazole-resistant isolates and the intI2 gene in 4 isolates; one gene cassette arrangements were identified among class 1 integrons (dfrA1 + aadA1) and among the class 2 integrons (dfrA1 + sat2 + aadA1). Among cefotaxime-resistant isolates, 16 belonged to A or B1 phylogenetic groups, while 11 isolates were classified into the D or B2 phylogroups. At least one virulence-associated gene (aer, fimA, or papC) was detected in 74·1% of the cefotaxime-resistant isolates. 4. Because ESBLs-producing bacteria are resistant to a broad range of β-lactams, infections caused by these organisms complicate therapy and limit treatment options.
- Published
- 2012
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6. Genetic characterization of extended-spectrum beta-lactamases in Escherichia coli isolates of pigs from a Portuguese intensive swine farm.
- Author
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Gonçalves A, Torres C, Silva N, Carneiro C, Radhouani H, Coelho C, Araújo C, Rodrigues J, Vinué L, Somalo S, Poeta P, and Igrejas G
- Subjects
- Animals, Electrophoresis, Gel, Pulsed-Field, Escherichia coli drug effects, Escherichia coli growth & development, Escherichia coli isolation & purification, Feces microbiology, Genes, MDR, Genetic Markers, Microbial Sensitivity Tests, Plasmids genetics, Polymerase Chain Reaction, Portugal, Drug Resistance, Bacterial, Escherichia coli enzymology, Escherichia coli Proteins genetics, Swine microbiology, beta-Lactamases genetics
- Abstract
There is a great concern by the emergence and the wide dissemination of extended-spectrum beta-lactamases (ESBLs) among animal Escherichia coli isolates. We intended to determinate the carriage level and type of ESBLs in E. coli obtained from fecal samples from pigs raised on an intensive pig farm in Portugal; further to characterize other associated resistance genes and their plasmid content, the phylogenetic groups, and the clonal relationship of ESBL-positive isolates. Sixty-five fecal samples were seeded in Levine media supplemented with cefotaxime for E. coli recovery. Susceptibility to 16 antimicrobial agents was performed by disk diffusion agar. ESBL-phenotypic detection was carried out by double-disk test; and the presence of the genes encoding TEM, OXA, SHV, and CTX-M type beta-lactamases was studied by polymerase chain reaction and sequencing. Other mechanisms of antimicrobial resistance and phylogenetic groups were also determined. Clonal relationship was performed by pulsed-field gel electrophoresis. ESBL-producing E. coli isolates were detected in 16 fecal samples, and one isolate per sample was studied. The CTX-M-1 type ESBL was detected in the 16 isolates. The gene encoding TEM-1 was identified to be associated with eight CTX-M-1-positive isolates. The tet(A) gene was found in 12 of 14 tetracycline-resistant isolates, and the aadA or strA-strB genes were found in the streptomycin-resistant isolates. Fourteen and two ESBL-containing isolates belonged to A and B1 phylogenetic groups, respectively. Clonal relationship of ESBL-containing isolates identified seven unrelated patterns. Swine represent an important reservoir of ESBL-containing E. coli isolates, especially of the CTX-M-1 type.
- Published
- 2010
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7. Genetic detection of extended-spectrum beta-lactamase-containing Escherichia coli isolates from birds of prey from Serra da Estrela Natural Reserve in Portugal.
- Author
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Pinto L, Radhouani H, Coelho C, Martins da Costa P, Simões R, Brandão RM, Torres C, Igrejas G, and Poeta P
- Subjects
- Animals, Escherichia coli genetics, Escherichia coli isolation & purification, Escherichia coli Infections microbiology, Feces microbiology, Portugal, Raptors, Bird Diseases microbiology, Escherichia coli enzymology, Escherichia coli Infections veterinary, Escherichia coli Proteins genetics, beta-Lactamases genetics
- Abstract
Extended-spectrum beta-lactamase-containing Escherichia coli isolates were detected in 32 of 119 fecal samples (26.9%) from birds of prey at Serra da Estrela, and these isolates contained the following beta-lactamases: CTX-M-1 (n = 13), CTX-M-1 plus TEM-1 (n = 14), CTX-M-1 plus TEM-20 (n = 1), SHV-5 (n = 1), SHV-5 plus TEM-1 (n = 2), and TEM-20 (n = 1).
- Published
- 2010
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8. Detection of Escherichia coli harbouring extended-spectrum {beta}-lactamases of the CTX-M classes in faecal samples of common buzzards (Buteo buteo).
- Author
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Radhouani H, Pinto L, Coelho C, Gonçalves A, Sargo R, Torres C, Igrejas G, and Poeta P
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- Animals, Drug Resistance, Bacterial, Genes, Bacterial, Integrons, Microbial Sensitivity Tests, beta-Lactamases classification, Escherichia coli enzymology, Escherichia coli isolation & purification, Falconiformes microbiology, Feces microbiology, beta-Lactamases biosynthesis
- Published
- 2010
- Full Text
- View/download PDF
9. Wild boars as reservoirs of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli of different phylogenetic groups.
- Author
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Poeta P, Radhouani H, Pinto L, Martinho A, Rego V, Rodrigues R, Gonçalves A, Rodrigues J, Estepa V, Torres C, and Igrejas G
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- Animals, Bacterial Typing Techniques, DNA, Bacterial genetics, Disease Reservoirs microbiology, Drug Resistance, Multiple, Bacterial genetics, Escherichia coli classification, Escherichia coli genetics, Feces microbiology, Genes, Bacterial, Microbial Sensitivity Tests, Portugal, Escherichia coli isolation & purification, Escherichia coli Proteins genetics, Phylogeny, Sus scrofa microbiology, beta-Lactamases genetics
- Abstract
ESBL-producing E. coli isolates have been isolated from eight of seventy seven faecal samples (10.4%) of wild boars in Portugal. The ESBL types identified by PCR and sequencing were bla(CTX-M-1) (6 isolates) and bla(CTX-M-1) + bla(TEM1-b) (2 isolates). Further resistance genes detected included tet (A) or tet (B) (in three tetracycline-resistant isolates), aad A (in three streptomycin-resistant isolates), cml A (in one chloramphenicol-resistant isolate), sul 1 and/or sul 2 and/or sul 3 (in all sulfonamide-resistant isolates). The intI 1 gene encoding class 1 integrase was detected in all ESBL-producing E. coli isolates. One isolate also carried the intI 2 gene, encoding class 2 integrase. The ESBL-producing E. coli isolates could be assigned to phylogenetic groups B1 (3 isolates), B2 (3 isolates) or A (2 isolates). Amino acid change in GyrA protein (Ser83Leu or Asp87Tyr) was detected in three nalidixic acid-resistant and ciprofloxacin-susceptible isolates. Two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC (Ser80Ile) were identified in two nalidixic acid- and ciprofloxacin-resistant isolates. As evidenced by this study wild boars could be a reservoir of antimicrobial resistance genes.
- Published
- 2009
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10. Genetic detection of extended-spectrum beta-lactamase-containing Escherichia coli isolates and vancomycin-resistant enterococci in fecal samples of healthy children.
- Author
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Guimarães B, Barreto A, Radhouani H, Figueiredo N, Gaspar E, Rodrigues J, Torres C, Igrejas G, and Poeta P
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- Adolescent, Anti-Bacterial Agents pharmacology, Child, Child, Preschool, Enterococcus drug effects, Enterococcus enzymology, Enterococcus genetics, Escherichia coli drug effects, Escherichia coli enzymology, Escherichia coli genetics, Genotype, Humans, Infant, Microbial Sensitivity Tests, Phenotype, beta-Lactamases metabolism, Disease Reservoirs, Enterococcus isolation & purification, Escherichia coli isolation & purification, Feces microbiology, Vancomycin Resistance, beta-Lactamases genetics
- Abstract
One hundred twelve fecal samples of healthy children were recovered in Portugal during October 2007 and February 2008 and were tested for extended-spectrum beta-lactamases (ESBLs) containing Escherichia coli isolates and vancomycin-resistant enterococci (VRE). Three of the 112 fecal samples (2.7%) harbored ESBL-positive E. coli isolates and the bla(CTX-M-1), bla(TEM-52), and bla(SHV-12) genes were identified in these isolates. The bla(TEM-52)-containing isolate showed a phenotype of multiresistance that included fluoroquinolones, tetracycline, trimethoprim-sulfamethoxazole, and chloramphenicol; sul1, sul3, and cmlA genes were detected in this isolate, in addition to two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC protein (Ser80Ile). The ESBL isolates corresponded to phylogroup A (one isolate), B1 (one isolate), and D (one isolate). vanA-containing Escherichia faecium isolates were detected in 13 of the 112 fecal samples (11.6%), and vanC-1 isolates were found in 2 samples. A diversity of resistance genes [(tet(M), tet(L), erm(B), aph(3')-IIIa, ant(6)-Ia, catA, and vat(E)] were found in VRE isolates. These results show that the intestinal tract of healthy children constitutes a reservoir of ESBL-containing E. coli and VRE isolates.
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- 2009
- Full Text
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11. Seagulls of the Berlengas natural reserve of Portugal as carriers of fecal Escherichia coli harboring CTX-M and TEM extended-spectrum beta-lactamases.
- Author
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Poeta P, Radhouani H, Igrejas G, Gonçalves A, Carvalho C, Rodrigues J, Vinué L, Somalo S, and Torres C
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- Animals, Escherichia coli genetics, Escherichia coli isolation & purification, Escherichia coli Infections microbiology, Feces microbiology, Genes, Bacterial, Portugal, beta-Lactam Resistance, beta-Lactamases genetics, Carrier State microbiology, Charadriiformes microbiology, Escherichia coli drug effects, Escherichia coli Infections veterinary, beta-Lactamases biosynthesis
- Abstract
Escherichia coli isolates containing the following extended-spectrum beta-lactamases have been detected in 11 of 57 fecal samples (19.3%) in Berlengas Island seagulls: TEM-52 (eight isolates), CTX-M-1 (one isolate), CTX-M-14a (one isolate), and CTX-M-32 (one isolate). Most of the extended-spectrum beta-lactamase-positive isolates harbored class 1 or class 2 integrons, which included different antibiotic resistance gene cassettes.
- Published
- 2008
- Full Text
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