1. Spatiotemporal regulation of galectin-1-induced T-cell death in lamina propria from Crohn’s disease and ulcerative colitis patients
- Author
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Gabriel A. Rabinovich, Karina Mariño, Cecilia Isabel Muglia, Renata Curciarello, Gustavo Correa, Paolo Biancheri, Martin Yantorno, Antonio Di Sabatino, Alicia M. Sambuelli, Thomas T. MacDonald, Marta A. Toscano, Luciano Gastón Morosi, Guillermo Horacio Docena, Rodrigo Papa-Gobbi, and Andrés Rocca
- Subjects
0301 basic medicine ,Peanut agglutinin ,Cancer Research ,Clinical Biochemistry ,Pharmaceutical Science ,Inflammation ,Inflammatory bowel disease ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Pharmacology ,Lamina propria ,Crohn's disease ,biology ,business.industry ,Biochemistry (medical) ,Cell Biology ,medicine.disease ,Ulcerative colitis ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Immunology ,biology.protein ,Immunohistochemistry ,medicine.symptom ,business ,CD8 - Abstract
Inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), is characterized by chronic, relapsing intestinal inflammation. Galectin-1 (Gal-1) is an endogenous lectin with key pro-resolving roles, including induction of T-cell apoptosis and secretion of immunosuppressive cytokines. Despite considerable progress, the relevance of Gal-1-induced T-cell death in inflamed tissue from human IBD patients has not been ascertained. Intestinal biopsies and surgical specimens from control patients (n = 52) and patients with active or inactive IBD (n = 97) were studied. Gal-1 expression was studied by RT-qPCR, immunoblotting, ELISA and immunohistochemistry. Gal-1-specific ligands and Gal-1-induced apoptosis of lamina propria (LP) T-cells were determined by TUNEL and flow cytometry. We found a transient expression of asialo core 1-O-glycans in LP T-cells from inflamed areas (p
- Published
- 2021