1. Changes in DNA-binding activity of transcription factors in the kidney of mice exposed to cadmium.
- Author
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Lee JY, Mori C, Tokumoto M, and Satoh M
- Subjects
- Animals, Dose-Response Relationship, Drug, Female, Mice, Inbred C57BL, Time Factors, Transcription Factors genetics, Mice, Cadmium toxicity, DNA metabolism, Gene Expression drug effects, Kidney diagnostic imaging, Kidney metabolism, Protein Binding drug effects, Transcription Factors metabolism
- Abstract
Cadmium (Cd) is a toxic heavy metal, long-term exposure to which causes renal damage associated with disruption in gene expression. Transcription factors whose activities were altered in the kidneys of mice exposed to Cd for 3 months were assessed using protein/DNA-binding assays. Female C57BL/6J mice were exposed to 300 ppm Cd in the diet for 3 months. Nuclear extracts of kidney were used for protein/DNA-binding assays. The concentration of Cd was approximately 100 ppm in mouse kidney, a level that did not induce renal toxicity. Among the 345 transcription factors evaluated, five transcription factors showed over a two-fold increase in their activities and 14 transcription factors showed a half-fold change in their activities after Cd exposure. These findings may provide new information about the causative transcription factors associated with Cd renal toxicity.
- Published
- 2021
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