1,607 results on '"*ACROLEIN"'
Search Results
2. Bioanalysis of Stress Biomarkers through Sensitive HILIC-MS/MS Method: A Stride toward Accurate Quantification of MDA, ACR, and CTA.
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Thakkar, Harsh, Gangakhedkar, Shriya, and Shah, Ravi P.
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Quantifying reactive aldehyde biomarkers, such as malondialdehyde, acrolein, and crotonaldehyde, is the most preferred approach to determine oxidative stress. However, reported analytical methods lack specificity for accurately quantifying these aldehydes as certain methodologies may produce false positive results due to harsh experimental conditions. Thus, in this research work, a novel HILIC-MS/MS method with endogenous histidine derivatization is developed, which proves to have higher specificity and reproducibility in quantifying these aldehydes from the biological matrix. To overcome the reactivity of aldehyde, endogenous histidine is used for its derivatization. The generated adduct is orthogonally characterized by NMR and LC-HRMS. The method employed a hydrophilic HILIC column and multiple reaction monitoring (MRM) to accurately quantify these reactive aldehydes. The developed method is an unequivocal solution for quantifying stress in in vivo and in vitro studies. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Acrolein Induces Changes in Cell Membrane and Cytosol Proteins of Erythrocytes.
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Kopera, Michal, Gwozdzinski, Krzysztof, and Pieniazek, Anna
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MEMBRANE proteins , *ERYTHROCYTE membranes , *ACROLEIN , *ERYTHROCYTES , *SULFHYDRYL group , *CIGARETTE smoke , *THIOLS - Abstract
High concentrations of acrolein (2-propenal) are found in polluted air and cigarette smoke, and may also be generated endogenously. Acrolein is also associated with the induction and progression of many diseases. The high reactivity of acrolein towards the thiol and amino groups of amino acids may cause damage to cell proteins. Acrolein may be responsible for the induction of oxidative stress in cells. We hypothesized that acrolein may contribute to the protein damage in erythrocytes, leading to the disruption of the structure of cell membranes. The lipid membrane fluidity, membrane cytoskeleton, and osmotic fragility were measured for erythrocytes incubated with acrolein for 24 h. The levels of thiol, amino, and carbonyl groups were determined in cell membrane and cytosol proteins. The level of non-enzymatic antioxidant potential (NEAC) and TBARS was also measured. The obtained research results showed that the exposure of erythrocytes to acrolein causes changes in the cell membrane and cytosol proteins. Acrolein stiffens the cell membrane of erythrocytes and increases their osmotic sensitivity. Moreover, it has been shown that erythrocytes treated with acrolein significantly reduce the non-enzymatic antioxidant potential of the cytosol compared to the control. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Puerarin alleviates acrolein‐induced atherosclerosis by activating the MYH9‐mediated SIRT1/Nrf2 cascade to inhibit the activation of inflammasome.
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Li, XiaoNing, Li, YeTing, Jiao, HuiHui, Wang, AiTing, Zheng, Man, Xiang, ChunYan, and Zhang, FengLei
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Puerarin (Pue) has significant antioxidant and anti‐inflammatory properties. This work was designed to clarify and investigate the potential mechanisms of Pue in atherosclerosis (AS) progression.In vivo, acrolein (Acr) was inhaled through drinking water to construct AS model. In vitro, CCK‐8 assay and lactate dehydrogenase (LDH) assay kit were used to detect cell viability. Apoptosis was detected by flow cytometry. The content of malondialdehyde (MDA) was determined by commercial kit, the level of inflammatory factors was detected by ELISA, and proteins were determined by western blot. Pue administration could effectively reduce blood lipid level in Acr‐fed mice. Pue suppressed oxidative stress, the formation of atherosclerotic plaques, and the process of aortic histological changes. Pue pretreatment decreased MDA in HUVECs and maintained the activity of antioxidant enzymes. Pue upregulated SIRT1/Nrf2 cascade in HUVECs. Pue increased MYH9 and inhibited NLRP3 inflammasome‐related proteins, and the inhibition of MYH9 significantly impaired Pue‐induced Nrf2 activation. Moreover, HUVEC cytotoxicity and apoptosis are alleviated by Pue, in addition to NLRP3‐mediated pyroptosis in HUVECs induced by Acr. MYH9 inhibitors effectively suppressed the pyroptosis induced by Acr and prevented injury to HUVECs. In addition, Pue promoted SIRT1/Nrf2 cascade activation in HUVECs. Pue may alleviate Acr‐induced AS by activating the MYH9‐mediated SIRT1/Nrf2 cascade to inhibit inflammasome activation. [ABSTRACT FROM AUTHOR]
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- 2024
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5. A Comparative Study of the Effects of Electronic Cigarette and Traditional Cigarette on the Pulmonary Functions of C57BL/6 Male Mice.
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Zhao, Han-Zhi, Guo, Zi-Wei, Wang, Zhang-Li, Wang, Chen, Luo, Xian-Yu, Han, Ning-Ning, Li, Chen-Rui, Zheng, Hua-Dong, Hui, Zi-Yi, Long, Yang, Zhao, Yan-Lei, Li, Qiu-Jin, Wang, Sheng-Yu, and Zhang, Guang-Wei
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Introduction Electronic cigarettes (E-cigs) are in a controversial state. Although E-cig aerosol generally contains fewer harmful substances than smoke from burned traditional cigarettes, aerosol along with other compounds of the E-cigs may also affect lung functions and promote the development of lung-related diseases. We investigated the effects of E-cig on the pulmonary functions of male C57BL/6 mice and reveal the potential underlying mechanisms. Methods A total of 60 male C57BL/6 mice were randomly divided into four groups. They were exposed to fresh-air, traditional cigarette smoke, E-cig vapor with 12 mg/mL of nicotine, and E-cig with no nicotine for 8 weeks. Lung functions were evaluated by using quantitative analysis of the whole body plethysmograph, FlexiVent system, lung tissue histological and morphometric analysis, and RT-PCR analysis of mRNA expression of inflammation-related genes. In addition, the effects of nicotine and acrolein on the survival rate and DNA damage were investigated using cultured human alveolar basal epithelial cells. Results Exposure to E-cig vapor led to significant changes in lung functions and structures including the rupture of the alveolar cavity and enlarged alveolar space. The pathological changes were also accompanied by increased expression of interleukin-6 and tumor necrosis factor-α. Conclusions The findings of the present study indicate that the safety of E-cig should be further evaluated. Implications Some people currently believe that using nicotine-free E-cigs is a safe way to smoke. However, our research shows that E-cigs can cause lung damage regardless of whether they contain nicotine. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Radical intermediates and stable products in acrolein pyrolysis.
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Muzika, Michael, Genossar-Dan, Nadav, Fux, Dana, Har Lavan, Shani, Zamir, Uri, Rozenberg, Illya, Hemberger, Patrick, and Baraban, Joshua H.
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RADICALS (Chemistry) , *ACROLEIN , *FOREST fires , *METHYL radicals , *HIGH temperature chemistry , *PYROLYSIS - Abstract
Acrolein is a commonly encountered pollutant of health concern, yet the processes that decompose acrolein are poorly understood. Frequently used in industrial synthesis, it is also a byproduct of combustion and other high temperature processes, especially those involving biological substances, e.g., forest fires, deep frying, or cigarette smoke. Despite the need for investigation of the chemical behavior of acrolein at high temperatures, little experimental work exists on decomposition of acrolein using apparatus capable of observing reactive species. Here, the pyrolysis of acrolein was studied at temperatures of up to 1700 K. We identified radicals and other unstable species produced in the early reaction stages, including vinyl radical, methyl radical, and methyl ketene. Detection of these reactive intermediates, and indirect evidence on the formation of others, reveals reaction pathways to stable species, including carbon monoxide, ethylene, and acetylene. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Metallic radionuclide-labeled tetrameric 2,6-diisopropylphenyl azides for cancer treatment.
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Ode, Yudai, Pradipta, Ambara R., Ishiwata, Akihiro, Nambu, Akihiro, Ohnuki, Kazunobu, Mizuma, Hiroshi, Haba, Hiromitsu, and Tanaka, Katsunori
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CANCER treatment , *AZIDES , *RADIOISOTOPES , *RADIONUCLIDE imaging , *ACROLEIN , *CANCER cells - Abstract
This study proposes a new method for radionuclide therapy that involves the use of oligomeric 2,6-diisopropylphenyl azides and a chelator to form stable complexes with metallic radionuclides. The technique works by taking advantage of the endogenous acrolein produced by cancer cells. The azides react with the acrolein to give a diazo derivative that immediately attaches to the nearest organelle, effectively anchoring the radionuclide within the tumor. Preliminary in vivo experiments were conducted on a human lung carcinoma xenograft model, demonstrating the feasibility of this approach for cancer treatment. [ABSTRACT FROM AUTHOR]
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- 2024
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8. Exposure to polycyclic aromatic hydrocarbons, volatile organic compounds, and tobacco-specific nitrosamines and incidence of esophageal cancer.
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Etemadi, Arash, Poustchi, Hossein, Chang, Cindy M, Calafat, Antonia M, Blount, Benjamin C, Bhandari, Deepak, Wang, Lanqing, Roshandel, Gholamreza, Alexandridis, Apostolos, Botelho, Julianne Cook, Xia, Baoyun, Wang, Yuesong, Sosnoff, Connie S, Feng, Jun, Nalini, Mahdi, Khoshnia, Masoud, Pourshams, Akram, Sotoudeh, Masoud, Gail, Mitchell H, and Dawsey, Sanford M
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VOLATILE organic compounds , *POLYCYCLIC aromatic hydrocarbons , *NITROSOAMINES , *ESOPHAGEAL cancer , *SQUAMOUS cell carcinoma , *TOBACCO products - Abstract
Background Studying carcinogens in tobacco and nontobacco sources may be key to understanding the pathogenesis and geographic distribution of esophageal cancer. Methods The Golestan Cohort Study has been conducted since 2004 in a region with high rates of esophageal squamous cell carcinoma. For this nested study, the cases comprised of all incident cases by January 1, 2018; controls were matched to the case by age, sex, residence, time in cohort, and tobacco use. We measured urinary concentrations of 33 exposure biomarkers of nicotine, polycyclic aromatic hydrocarbons, volatile organic compounds, and tobacco-specific nitrosamines. We used conditional logistic regression to calculate odds ratios (ORs) and 95% confidence intervals for associations between the 90th vs the 10th percentiles of the biomarker concentrations and incident esophageal squamous cell carcinoma. Results Among individuals who did not currently use tobacco (148 cases and 163 controls), 2 acrolein metabolites, 2 acrylonitrile metabolites, 1 propylene oxide metabolite, and one 1,3-butadiene metabolite were significantly associated with incident esophageal squamous cell carcinoma (adjusted odds ratios between 1.8 and 4.3). Among tobacco users (57 cases and 63 controls), metabolites of 2 other volatile organic compounds (styrene and xylene) were associated with esophageal squamous cell carcinoma (OR = 6.2 and 9.0, respectively). In tobacco users, 2 tobacco-specific nitrosamines (NNN and N' -Nitrosoanatabine) were also associated with esophageal squamous cell carcinoma. Suggestive associations were seen with some polycyclic aromatic hydrocarbons (especially 2-hydroxynaphthalene) in nonusers of tobacco products and other tobacco-specific nitrosamines in tobacco users. Conclusion These novel associations based on individual-level data and samples collected many years before cancer diagnosis, from a population without occupational exposure, have important public health implications. [ABSTRACT FROM AUTHOR]
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- 2024
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9. Palladium-catalyzed allylation and carbonylation: access to allylhydrazones and allyl acylhydrazones.
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Liang, Qianqian, Cai, Yan, Jiang, Wenjun, Pang, Mengdi, Fan, Liming, and Zhang, Guoying
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CARBONYLATION , *ALLYLATION , *ACROLEIN , *ALLYL alcohol , *HYDRAZINE , *HYDRAZINES - Abstract
A palladium-catalyzed allylation of hydrazines with allyl alcohols and aldehydes was developed, enabling the syntheses of a series of allylhydrazones in good to excellent yields with high regioselectivity. Furthermore, the four-component tandem allylation carbonylation of hydrazines with allyl alcohols and aldehydes was established using the catalytic system, producing various allyl acylhydrazones. Additionally, the functionalized allyl acylhydrazones could be smoothly constructed with the catalytic system employing allylhydrazones as a partner. The catalytic system exhibited good functional tolerance with excellent regioselectivities and scaled-up capability, overcoming the limitations of chemoselectivity of the multicomponent transformation and poor conversion of the weak nucleophile. [ABSTRACT FROM AUTHOR]
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- 2024
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10. Insight into acrolein activation by P/B intramolecular frustrated Lewis pairs.
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Sinha, Swapan, Das, Subhra, and Giri, Santanab
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LEWIS pairs (Chemistry) , *CHEMICAL processes , *ACROLEIN , *CHEMICAL reactions , *REACTION forces , *RING formation (Chemistry) - Abstract
The study investigates the reactivity of a cyclic five‐membered intramolecular P/B frustrated Lewis pair towards acrolein through a cycloaddition reaction. Intrinsic reaction coordinate (IRC) calculations suggest the single‐step mechanism. It has been observed that the cycloaddition reaction occurs through a concerted mechanism in both the presence and absence of the catalyst. Analysis of reaction force and reaction electronic flux provides valuable information about the total work required and electronic activity along the IRC. Additionally, natural bonding orbital (NBO) analyses enrich the understanding of the mechanism in terms of the electron transfer process during the chemical reaction. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Tropospheric Photochemistry of 2-Butenedial: Role of the Triplet States, CO and Acrolein Formation, and the Experimentally Unidentified Carbonyl Compound—Theoretical Study.
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Maranzana, Andrea and Tonachini, Glauco
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CARBONYL compounds , *MALEIC anhydride , *ACROLEIN , *CYCLIC compounds , *PHOTOCHEMISTRY , *TROPOSPHERIC ozone - Abstract
Solar irradiation of 2-butenedial in the lower troposphere mainly produces isomeric ketene-enol (a key intermediate product), furanones, and maleic anhydride, the formation pathways of which were investigated in a previous study. The other main products were carbon monoxide and an experimentally unidentified carbonyl compound. This was the subject of the present study. The oxidative reaction mechanisms were studied using DFT calculations. Water intervention is found essential. Its addition and subsequent water-assisted isomerizations (an ene-gem-diol/enol and a carboxylic acid/enol form), followed by cyclization, lead to an interesting cyclic carbonyl compound, but this pathway appears to be rather energy demanding. An alternative implies water cooperation in a ketene-enol + carboxylic acid/enol addition that gives the relevant anhydride. The anhydride is proposed as a candidate for the experimentally unidentified carbonyl product. Regarding CO and acrolein formation, the role of the triplet states, as defined by the probability of intersystem crossing from the excited singlet state S1 to T2 and T1, is discussed. The T1 photolysis pathway connecting butenedial to propenal + CO was then defined. [ABSTRACT FROM AUTHOR]
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- 2024
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12. Acrolein suppresses anticancer drug-induced toxicity mediated by activating claudin-1 and Nrf2 axis in a spheroid model of human lung squamous cell carcinoma cells.
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Eguchi, Hiroaki, Yu, Yaqing, Matsunaga, Toshiyuki, Yoshino, Yuta, and Ikari, Akira
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NUCLEAR factor E2 related factor , *SQUAMOUS cell carcinoma , *NICOTINE , *ACROLEIN , *FETAL hemoglobin , *ALKALOIDS , *CLAUDINS - Abstract
Tobacco smoke contains various carcinogenic ingredients such as nicotine, acrolein, and benzopyrene; however, their effects on cancer treatment are not fully understood. Claudin-1 (CLDN1), a component of tight junctions, is involved in the increased resistance to anticancer drugs. In this study, we found that acrolein increases the mRNA and protein levels of CLDN1 in RERF-LC-AI cells derived from human lung squamous cell carcinoma (SCC). Acrolein increased the p-extracellular signal-regulated kinase (ERK) 1/2 levels without affecting the p-Akt level. The acrolein-induced elevation of CLDN1 expression was attenuated by U0126, a mitogen-activated protein kinase kinas (MEK) inhibitor. These results indicate that the activation of MEK/ERK pathway is involved in the acrolein-induced elevation of CLDN1 expression. In a spheroid model, acrolein suppressed the accumulation and toxicity of doxorubicin (DXR), which were rescued by CLDN1 silencing. The acrolein-induced effects were also observed in lung SCC-derived EBC-1 and LK-2 cells. Acrolein also increased the expression level of nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor that regulates antioxidant and detoxifying genes, which were inhibited by CLDN1 silencing. In spheroid cells, the levels of reactive oxygen species were enhanced by acrolein, which was inhibited by CLDN1 silencing. Taken together, acrolein may reduce the anticancer drug-induced toxicity in human lung SCC cells mediated by high CLDN1 expression followed by the upregulation of Nrf2 signaling pathway. [Display omitted] • Claudin-1 expression is increased by acrolein in human lung squamous cell carcinoma cells. • The activation of MEK/ERK pathway is involve in the acrolein-induced elevation of claudin-1 expression. • Acrolein increases reactive oxygen species production and Nrf2 expression. • Acrolein suppresses the toxicity of anticancer drugs mediated by high claudin-1 expression followed by the upregulation of Nrf2 signal. [ABSTRACT FROM AUTHOR]
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- 2024
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13. Utility of structural engineering on the monitoring of acrolein by aluminum nitride nano tube.
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Al- Bayati, Alaa Dhari Jawad, Hasoon, Ahmed, Alanssari, Ali Ihsan, Al-Thamir, Mohaimen, Ismael, Nadia Salim, Hussein, Mohamed J., and Alawadi, Ahmed H. R.
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ALUMINUM nitride , *STRUCTURAL health monitoring , *ACROLEIN , *GIBBS' free energy , *INTERMOLECULAR forces - Abstract
Context: The study delves into the adsorption process of acrolein (AC) onto both an untainted and a titanium-doped aluminum nitride nanotube (AlNNT) using computations based on density functional theory. As AC approaches the pure AlNNT, it exhibits a calculated adsorption energy (Ead) of -5.3 kcal/mol, underscoring the feeble nature of the adsorption. Furthermore, there has been very little change to the AlNNT's natural electrical characteristics. On the contrary, the introduction of titanium (Ti) enhances the performance of AlNNT, rendering it more susceptible and reactive to AC signals. Analyzing the conventional Gibbs free energy of formation computationally, we ascertain that replacing a nitrogen (N) atom with a titanium (Ti) atom within the aluminum nitride nanotube (AlNNT) structure presents a more advantageous prospect. Notably, there is a substantial alteration in the energy of adsorption (Ead) for AC as a Ti atom is incorporated onto the AlNNT surface, resulting in a shift from -5.3 to -24.6 kcal/mol. Methods: Energy calculations and geometric optimizations were conducted utilizing the dispersion-augmented B3LYP method, known as B3LYP-D. In this approach, Grimme's dispersion term, referred to as the "D" term, was employed to account for dispersion forces. The basis set adopted was 6–31 + + G** (d), and all computational procedures were executed using the GAMESS software program. Following the incorporation of titanium (Ti), this adjustment leads to a substantial enhancement in sensing capability, reaching a value of 93.7. This indicates an improved electrical conductivity of the aluminum nitride nanotube (AlNNT). Remarkably, the Ti-doped AlNNT demonstrates the ability to detect AC distinctly, even in the presence of HCN, formaldehyde, ethanol, toluene, and acetone. The swift recovery process becomes evident as AC desorbs from the surface of Ti-doped AlNNT, with a calculated recovery time of 14.0 s. [ABSTRACT FROM AUTHOR]
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- 2024
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14. Exploring azomethine ylides reactivity with acrolein through cycloaddition reaction and computational antiviral activity assessment against hepatitis C virus.
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Abdessadak, Oumayma, Kandwal, Pankaj, Alaqarbeh, Marwa, Tabti, Kamal, Sbai, Abdelouahid, Ajana, Mohammed Aziz, Lakhlifi, Tahar, and Bouachrine, Mohammed
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YLIDES , *HEPATITIS C virus , *RING formation (Chemistry) , *MOLECULAR orbitals , *ACROLEIN , *MOLECULAR dynamics , *SCHIFF bases - Abstract
Context: The regioselectivity and diastereoselectivity of the 1,3-dipolar cycloaddition reaction between azomethine ylides and acrolein were investigated. The DFT studies revealed that the favored pathway leads to the formation of cis-cycloadduct pyrrolidine and these computational findings align with experimental observations. The cis-cycloadduct pyrrolidine product serves as an advanced intermediate in the synthesis of a hepatitis C virus inhibitor. For this, the antiviral activity of cis-cycloadduct pyrrolidine against cyclophilin A, the co-factor responsible for hepatitis C virus, was also evaluated through molecular docking simulations which revealed intriguing interactions and a high C-score, which were further confirmed by molecular dynamics simulations, demonstrating stability over a 100-ns simulation period. Furthermore, the cis-cycloadduct pyrrolidine exhibits favorable drug-like properties and a better ADMET profile compared to hepatitis C virus inhibitor. Methods: Chemical reactivity studies were performed using DFT method by the functional B3LYP at 6-31G (d, p) computational level by GAUSSIAN 16 program. Frontal molecular orbitals theory used to investigate HOMO/LUMO interactions between azomethine ylides and acrolein. Findings of this approach were confirmed by global reactivity indices and electron displacement was investigated based on Fukui functions. Furthermore, the activation energies were determined after frequency calculations using TS Berny algorithm and transition states were confirmed by the presence of a single imaginary frequency. Moreover, antiviral activity of cis-cycloadduct was explored through molecular docking using Surflex-Dock suite SYBYL X 2.0, and molecular dynamics simulation using GROMACS program. Finally, drug-like properties were investigated with SwissADME and ADMETlab 2.0. [ABSTRACT FROM AUTHOR]
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- 2024
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15. The Recycling of Substandard Rocket Fuel N,N-Dimethylhydrazine via the Involvement of Its Hydrazones Derived from Glyoxal, Acrolein, Metacrolein, Crotonaldehyde, and Formaldehyde in Organic Synthesis.
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Ivanova, Elizaveta, Osipova, Margarita, Vasilieva, Tatyana, Eremkin, Alexey, Markova, Svetlana, Zazhivihina, Ekaterina, Smirnova, Svetlana, Mitrasov, Yurii, and Nasakin, Oleg
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ROCKET fuel , *ORGANIC synthesis , *ACROLEIN , *GLYOXAL , *CROTONALDEHYDE , *FORMALDEHYDE , *HYDRAZONES - Abstract
"Heptil" (unsymmetrical dimethylhydrazine—UDMH) is extensively employed worldwide as a propellant for rocket engines. However, UDMH constantly loses its properties as a result of its continuous and uncontrolled absorption of moisture, which cannot be rectified. This situation threatens its long-term usability. UDMH is an exceedingly toxic compound (Hazard Class 1), which complicates its transportation and disposal. Incineration is currently the only method used for its disposal, but this process generates oxidation by-products that are even more toxic than the original UDMH. A more benign approach involves its immediate reaction with a formalin solution to form 1,1–dimethyl-2-methylene hydrazone (MDH), which is significantly less toxic by an order of magnitude. MDH can then be polymerized under acidic conditions, and the resulting product can be burned, yielding substantial amounts of nitrogen oxides. This review seeks to shift the focus of MDH from incineration towards its application in the synthesis of relatively non-toxic and readily available analogs of various pharmaceutical substances. We aim to bring the attention of the international chemical community to the distinctive properties of MDH, as well as other hydrazones (such as glyoxal, acrolein, crotonal, and meta-crolyl), wherein each structural fragment can initiate unique transformations that have potential applications in molecular design, pharmaceutical research, and medicinal chemistry. [ABSTRACT FROM AUTHOR]
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- 2023
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16. Serum metabolome and liver transcriptome reveal acrolein inhalation-induced sex-specific homeostatic dysfunction.
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Alewel, Devin I., Rentschler, Katherine M., Jackson, Thomas W., Schladweiler, Mette C., Astriab-Fisher, Anna, Evansky, Paul A., and Kodavanti, Urmila P.
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ACROLEIN , *LIVER , *TRANSCRIPTOMES , *METABOLIC detoxification , *GUT microbiome , *AMINO acids , *METABOLOMICS , *MICROBIAL metabolites - Abstract
Acrolein, a respiratory irritant, induces systemic neuroendocrine stress. However, peripheral metabolic effects have not been examined. Male and female WKY rats were exposed to air (0 ppm) or acrolein (3.16 ppm) for 4 h, followed by immediate serum and liver tissue collection. Serum metabolomics in both sexes and liver transcriptomics in males were evaluated to characterize the systemic metabolic response. Of 887 identified metabolites, > 400 differed between sexes at baseline. An acrolein biomarker, 3-hydroxypropyl mercapturic acid, increased 18-fold in males and 33-fold in females, indicating greater metabolic detoxification in females than males. Acrolein exposure changed 174 metabolites in males but only 50 in females. Metabolic process assessment identified higher circulating free-fatty acids, glycerols, and other lipids in male but not female rats exposed to acrolein. In males, acrolein also increased branched-chain amino acids, which was linked with metabolites of nitrogen imbalance within the gut microbiome. The contribution of neuroendocrine stress was evident by increased corticosterone in males but not females. Male liver transcriptomics revealed acrolein-induced over-representation of lipid and protein metabolic processes, and pathway alterations including Sirtuin, insulin-receptor, acute-phase, and glucocorticoid signaling. In sum, acute acrolein inhalation resulted in sex-specific serum metabolomic and liver transcriptomic derangement, which may have connections to chronic metabolic-related diseases. [ABSTRACT FROM AUTHOR]
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- 2023
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17. Boryl Acrylaldehyde: An Elusive Member of the α-Boryl Aldehyde Class of Reagent.
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Trofimova, Alina, Brien, Chelsey, Trinchera, Piera, Tien, Chieh-Hung, and Yudin, Andrei K.
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ACROLEIN , *ALDEHYDES , *NORMAL-phase chromatography , *APROTIC solvents - Abstract
19 Boryl MIDA Aldehyde 3c To an oven-dried round-bottom flask equipped with a magnetic stir bar was added MIDA boronate 8 (100 mg, 0.44 mmol, 1.0 equiv) dissolved in anhydrous MeCN (0.1 M). Boron, boryl aldehydes, hemilability, amphoteric molecules, oximes, boryl hydrazones, boryl Keywords: boron; boryl aldehydes; amphoteric molecules; hemilability; boryl; oximes; boryl hydrazones EN boron boryl aldehydes amphoteric molecules hemilability boryl oximes boryl hydrazones 2239 2243 5 10/31/23 20231127 NES 231127 Graph Matteson homologation is a powerful method for the synthesis of organoboron compounds. 22 Boryl MIDA Oxime 9 To a round-bottom flask equipped with a magnetic stir bar was added boryl MIDA aldehyde 3c (23 mg, 0.1 mmol, 1.0 equiv). [Extracted from the article]
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- 2023
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18. Access to 2‐Sulfonyl‐4‐arylphenols and 2,6‐Bis‐sulfonyl 5‐Aryl‐4‐arylidene‐2‐cyclohexenones via Metal Triflates‐Catalyzed Domino Cyclocondensation of 1,3‐Bis‐sulfonylacetones and 3‐Arylacroleins
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Chang, Meng‐Yang and Hsueh, Nai‐Chen
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METALS , *ANNULATION , *CYCLOHEXENONES - Abstract
Herein, metal triflates–catalyzed one‐pot domino cyclocondensation of 1,3‐bis‐sulfonylacetones and 3‐arylacroleins via (3+3) or (3+2+1) annulation has been proposed to construct diversified 2‐sulfonyl‐4‐arylphenols and 2,6‐bis‐sulfonyl 5‐aryl‐4‐arylidene‐2‐cyclohexenones. In this effective reaction, C−C and C=C bonds are formed via a cascade process and 3‐arylacrolein acts as a C3 and C1 synthon during the formation of a six‐membered ring system under mild conditions. A plausible mechanism has been proposed and discussed. [ABSTRACT FROM AUTHOR]
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- 2023
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19. Polyamine-Derived Aminoaldehydes and Acrolein: Cytotoxicity, Reactivity and Analysis of the Induced Protein Modifications.
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Šebela, Marek and Rašková, Michaela
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POLYAMINES , *POLLUTANTS , *CYTOTOXINS , *ACROLEIN , *AMINE oxidase , *PROTEIN analysis - Abstract
Polyamines participate in the processes of cell growth and development. The degradation branch of their metabolism involves amine oxidases. The oxidation of spermine, spermidine and putrescine releases hydrogen peroxide and the corresponding aminoaldehyde. Polyamine-derived aminoaldehydes have been found to be cytotoxic, and they represent the subject of this review. 3-aminopropanal disrupts the lysosomal membrane and triggers apoptosis or necrosis in the damaged cells. It is implicated in the pathogenesis of cerebral ischemia. Furthermore, 3-aminopropanal yields acrolein through the elimination of ammonia. This reactive aldehyde is also generated by the decomposition of aminoaldehydes produced in the reaction of serum amine oxidase with spermidine or spermine. In addition, acrolein is a common environmental pollutant. It causes covalent modifications of proteins, including carbonylation, the production of Michael-type adducts and cross-linking, and it has been associated with inflammation-related diseases. APAL and acrolein are detoxified by aldehyde dehydrogenases and other mechanisms. High-performance liquid chromatography, immunochemistry and mass spectrometry have been largely used to analyze the presence of polyamine-derived aminoaldehydes and protein modifications elicited by their effect. However, the main and still open challenge is to find clues for discovering clear linkages between aldehyde-induced modifications of specific proteins and the development of various diseases. [ABSTRACT FROM AUTHOR]
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- 2023
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20. Acrolein increases the concentration of intracellular Zn 2 ⁺ by producing mitochondrial reactive oxygen species in A549 cells.
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Liu, Xueqi, Sun, Wenwu, Cao, Jianping, and Ma, Zhuang
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REACTIVE oxygen species , *ACROLEIN , *MITOCHONDRIA , *OCCUPATIONAL exposure , *FLUORESCENT probes - Abstract
Smoking or occupational exposure leads to low concentrations of acrolein on the surface of the airways. Acrolein is involved in the pathophysiological processes of various respiratory diseases. Reports showed that acrolein induced an increase in mitochondrial reactive oxygen species (mROS). Furthermore, exogenous H₂O₂ was found to increase intracellular Zn2⁺ concentration ([Zn2⁺]ᵢ). However, the specific impact of acrolein on changes in intracellular Zn2⁺ levels has not been fully investigated. Therefore, this study aimed to investigate the effects of acrolein on mROS and [Zn2⁺]ᵢ in A549 cells. We used Mito Tracker Red CM-H2Xros (MitoROS) and Fluozin-3 fluorescent probes to observe changes in mROS and intracellular Zn2⁺. The results revealed that acrolein increased [Zn2⁺]ᵢ in a time- and dose-dependent manner. Additionally, the production of mROS was observed in response to acrolein treatment. Subsequent experiments showed that the intracellular Zn2⁺ chelator TPEN could inhibit the acrolein-induced elevation of [Zn2⁺]ᵢ but did not affect the acrolein-induced mROS production. Conversely, the acrolein-induced elevation of mROS and [Zn2⁺]ᵢ were significantly decreased by the inhibitors of ROS formation (NaHSO₃, NAC). Furthermore, external oxygen free radicals increased both [Zn2⁺]ᵢ levels and mROS production. These results demonstrated that acrolein-induced elevation of [Zn2⁺]ᵢ in A549 cells was mediated by mROS generation, rather than through a pathway where [Zn2⁺]ᵢ elevation leads to mROS production. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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21. Electrochemically‐Driven Organocatalytic Enantioselective Oxidative Coupling of Tetrahydroisoquinolines and Acrylaldehyde.
- Author
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Zhang, Qi‐Ying, Li, Lu‐Xin, Wang, Song‐Lin, Ma, Jing, and Guo, Hai‐Ming
- Subjects
- *
TETRAHYDROISOQUINOLINES , *ACROLEIN , *OXIDATIVE coupling , *ORGANOCATALYSIS - Abstract
An electrochemically‐driven organocatalytic enantioselective oxidative coupling of tetrahydroisoquinolines and acrylaldehyde was developed. Various chiral C1‐alkenyl tetrahydroisoquinolines derivatives were obtained with 69–86% yields and 93:7–96:4 er. Notable features of this reaction include asymmetric organocatalysis (5.0 mol% β‐ICD as catalyst), electricity as the oxidant, air atmosphere, and undivided cell. This synthetic route offers access to various optically active C1‐substituted tetrahydroisoquinolines derivatives. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
22. Acrolein produced by glioma cells under hypoxia inhibits neutrophil AKT activity and suppresses anti-tumoral activities.
- Author
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Tsai, Hong-Chieh, Tong, Zhen-Jie, Hwang, Tsong-Long, Wei, Kuo-Chen, Chen, Pin-Yuan, Huang, Chiung-Yin, Chen, Ko-Ting, Lin, Ya-Jui, Cheng, Hsiao-Wei, and Wang, Hsiang-Tsui
- Subjects
- *
ACROLEIN , *GLIOMAS , *NEUTROPHILS , *TUMOR growth , *HYPOXEMIA , *DNA adducts , *COBALT chloride - Abstract
Acrolein, which is the most reactive aldehyde, is a byproduct of lipid peroxidation in a hypoxic environment. Acrolein has been shown to form acrolein-cysteine bonds, resulting in functional changes in proteins and immune effector cell suppression. Neutrophils are the most abundant immune effector cells in circulation in humans. In the tumor microenvironment, proinflammatory tumor-associated neutrophils (TANs), which are termed N1 neutrophils, exert antitumor effects via the secretion of cytokines, while anti-inflammatory neutrophils (N2 neutrophils) support tumor growth. Glioma is characterized by significant tissue hypoxia, immune cell infiltration, and a highly immunosuppressive microenvironment. In glioma, neutrophils exert antitumor effects early in tumor development but gradually shift to a tumor-supporting role as the tumor develops. However, the mechanism of this anti-to protumoral switch in TANs remains unclear. In this study, we found that the production of acrolein in glioma cells under hypoxic conditions inhibited neutrophil activation and induced an anti-inflammatory phenotype by directly reacting with Cys310 of AKT and inhibiting AKT activity. A higher percentage of cells expressing acrolein adducts in tumor tissue are associated with poorer prognosis in glioblastoma patients. Furthermore, high-grade glioma patients have increased serum acrolein levels and impaired neutrophil functions. These results suggest that acrolein suppresses neutrophil function and contributes to the switch in the neutrophil phenotype in glioma. [Display omitted] • Glioma cells produce acrolein under hypoxic conditions. • Acrolein inhibits neutrophil activation and induces anti-inflammatory phenotype. • Acrolein suppresses the glioma tumor-killing activity of neutrophils. • GBM patients with higher acrolein adducts in tumors are related to poor prognosis. • Glioma patients have higher serum acrolein levels and impaired neutrophil functions. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
23. Innovative Solutions for Food Analysis: Microextraction Techniques in Lipid Peroxidation Product Detection.
- Author
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Custodio-Mendoza, Jorge A., Ares-Fuentes, Ana M., and Carro, Antonia M.
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- *
FOOD chemistry , *PEROXIDATION , *FLAVOR , *EXTRACTION techniques , *CARBONYL compounds , *LIPIDS , *FOOD quality , *ALCOHOLIC beverages - Abstract
Lipid peroxidation, the most aggressive reaction in food, results in the formation of reactive organic compounds that detrimentally impact food sensory qualities and consumers' health. While controlled lipid peroxidation can enhance flavors and appearance in certain foods, secondary peroxidation products lead to sensory deterioration in a variety of products, such as oils, alcoholic beverages, and meat. This publication reviews the use of modern analytical techniques for detecting and quantifying carbonyl compounds, i.e., secondary lipid peroxidation products. The paper focuses specifically on microextraction-based methods: dispersive liquid-liquid microextraction (DLLME), solid-phase microextraction (SPME), and gas-diffusion microextraction (GDME). These techniques offer efficient and sensitive approaches to extracting and quantifying lipid oxidation products and contribute to the understanding of oxidative deterioration in various food products. The review outlines recent advancements, challenges, and limitations in these microextraction techniques, as well as emphasizes the potential for further innovation and improvement in the field of food analysis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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24. In Silico Investigation of Al, Si and Ge Dopants Effect on Structural and Electrical Properties of Pristine B12N12 Nanocage Toward Acrolein Adsorption.
- Author
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Noormohammadbeigi, Motahareh, Kamalinahad, Saeedeh, Shamlouei, Hamid Reza, Mehr, Fatemeh Izadi, and Rajabi, Raman
- Subjects
- *
THERMODYNAMICS , *ACROLEIN , *ADSORPTION (Chemistry) , *ZINC oxide thin films , *SILICON alloys , *DENSITY of states , *DENSITY functional theory - Abstract
Interaction between acrolein molecule and pristine, (Al/Si/Ge)-doped B11N12 nanocage has been performed by density functional theory at PBEPBE/6–311 + G (d, p) level of theory in gas environment. Total and partial density of states, HOMO–LUMO distributions, adsorption and gap energies, and thermodynamic properties were computed for studied structures. Based on the present theoretical results, this adsorption process can effectively modify the electrical conductivity (ΔEg = 75.95%) of the nanocage with − 18.65 kcal.mol−1 calculated for adsorption energy relevant to the most stable configuration C3H4O/B12N12 (S1) which suggests potential application of this nanocluster as a great sensor in detection of acrolein molecule avoiding the necessity of doping or functionalizing. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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25. New Biotinylated GHK and Related Copper(II) Complex: Antioxidant and Antiglycant Properties In Vitro against Neurodegenerative Disorders.
- Author
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Tosto, Rita, Vecchio, Graziella, and Bellia, Francesco
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- *
NEURODEGENERATION , *PEPTIDES , *ALZHEIMER'S disease , *COPPER , *FLUORESCENT dyes , *COPPER compounds - Abstract
Neurodegenerative diseases affect millions of people worldwide. The failure of the enzymatic degradation, the oxidative stress, the dyshomeostasis of metal ions, among many other biochemical events, might trigger the pathological route, but the onset of these pathologies is unknown. Multi-target and multifunctional molecules could address several biomolecular issues of the pathologies. The tripeptide GHK, a bioactive fragment of several proteins, and the related copper(II) complex have been largely used for many purposes, from cosmetic to therapeutic applications. GHK derivatives were synthesized to increase the peptide stability and improve the target delivery. Herein we report the synthesis of a new biotin–GHK conjugate (BioGHK) through orthogonal reactions. BioGHK is still capable of coordinating copper(II), as observed by spectroscopic and spectrometric measurements. The spectroscopic monitoring of the copper-induced ascorbate oxidation was used to measure the antioxidant activity Cu(II)-BioGHK complex, whereas antiglycant activity of the ligand towards harmful reactive species was investigated using MALDI-TOF. The affinity of BioGHK for streptavidin was evaluated using a spectrophotometric assay and compared to that of biotin. Finally, the antiaggregant activity towards amyloid-β was evaluated using a turn-on fluorescent dye. BioGHK could treat and/or prevent several adverse biochemical reactions that characterize neurodegenerative disorders, such as Alzheimer's disease. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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26. Acrolein Induces Retinal Abnormalities of Alzheimer's Disease in Mice.
- Author
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Wang, Shuyi, Jiang, Xiuying, Peng, Weijia, Yang, Shuangjian, Pi, Rongbiao, and Zhou, Shiyou
- Subjects
- *
ALZHEIMER'S disease , *ACROLEIN , *CARDIOVASCULAR system , *RETINA , *MICE , *NERVE fibers , *AMYLOID beta-protein - Abstract
It is reported that retinal abnormities are related to Alzheimer's disease (AD) in patients and animal models. However, it is unclear whether the retinal abnormities appear in the mouse model of sporadic Alzheimer's disease (sAD) induced by acrolein. We investigated the alterations of retinal function and structure, the levels of β-amyloid (Aβ) and phosphorylated Tau (p-Tau) in the retina, and the changes in the retinal vascular system in this mouse model. We demonstrated that the levels of Aβ and p-Tau were increased in the retinas of mice from the acrolein groups. Subsequently, a decreased amplitudes of b-waves in the scotopic and photopic electroretinogram (ERG), decreased thicknesses of the retinal nerve fiber layer (RNFL) in the retina, and slight retinal venous beading were found in the mice induced by acrolein. We propose that sAD mice induced by acrolein showed abnormalities in the retina, which may provide a valuable reference for the study of the retina in sAD. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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27. Inhibition of Polyamine Catabolism Reduces Cellular Senescence.
- Author
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Uemura, Takeshi, Matsunaga, Miki, Yokota, Yuka, Takao, Koichi, and Furuchi, Takemitsu
- Subjects
- *
POLYAMINES , *CATABOLISM , *SPERMINE , *CELLULAR aging , *DNA damage , *PHYSICAL mobility , *AGING prevention - Abstract
The aging of the global population has necessitated the identification of effective anti-aging technologies based on scientific evidence. Polyamines (putrescine, spermidine, and spermine) are essential for cell growth and function. Age-related reductions in polyamine levels have been shown to be associated with reduced cognitive and physical functions. We have previously found that the expression of spermine oxidase (SMOX) increases with age; however, the relationship between SMOX expression and cellular senescence remains unclear. Therefore, we investigated the relationship between increased SMOX expression and cellular senescence using human-liver-derived HepG2 cells. Intracellular spermine levels decreased and spermidine levels increased with the serial passaging of cells (aged cells), and aged cells showed increased expression of SMOX. The levels of acrolein-conjugated protein, which is produced during spermine degradation, also increases. Senescence-associated β-gal activity was increased in aged cells, and the increase was suppressed by MDL72527, an inhibitor of acetylpolyamine oxidase (AcPAO) and SMOX, both of which are enzymes that catalyze polyamine degradation. DNA damage accumulated in aged cells and MDL72527 reduced DNA damage. These results suggest that the SMOX-mediated degradation of spermine plays an important role in cellular senescence. Our results demonstrate that cellular senescence can be controlled by inhibiting spermine degradation using a polyamine-catabolizing enzyme inhibitor. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
28. On the History of Developing Catalysis in Ukraine (1850s-1980s).
- Author
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Gamaliia, Vira, Zabuga, Artem, and Zabuga, Gennadii
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- *
CATALYST selectivity , *CATALYSIS , *PHYSICAL & theoretical chemistry , *CATALYST structure , *CHEMICAL industry , *ACRYLIC acid - Abstract
The article is dedicated to the history of developing highly effective catalysts in the leading scientific institutions of Ukraine and explores the prerequisites for developing theories in physical chemistry, in particular those related to kinetics and catalysis. It highlights the significance of scientific discoveries at the turn of the 19th and 20th century and their application by native scientists to advance theoretical development in the field of chemistry. Special attention is paid to the works of Lev Pisarzhevskii, focusing on his advancements in electronic chemistry and, in particular, the electronic theory of catalysis. The article also outlines current challenges in creating highly efficient catalysts for the chemical and light industry, emphasizing the importance of such indicators of catalysts as activity and selectivity. Drawing on historical, scientific and patent data, the study investigates the process of creating a highly efficient catalyst for obtaining acrylic acid from acrolein. This catalyst holds a great practical importance for the production of various polymers in industrial conditions. It is shown that, as a result of research conducted by native scientists of the Institute of Physical Chemistry of the Academy of Sciences of the Ukrainian SSR and the Chemistry Department of Kyiv State University, the catalyst K-2-5 was developed. The catalyst has good indicators for the industrial production of acrylic acid from acrolein. The authors also highlight works studying the properties of the obtained catalyst, specifically its porous structure, which is an important factor in catalytic processes. They extensively focus on the kinetic indicators of catalytic reactions that occur when using this catalyst. The article also emphasizes the relevance of these developments for advancing research in catalysis and chemical industrial production. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
29. The contribution of initial concussive forces and resulting acrolein surge to β-amyloid accumulation and functional alterations in neuronal networks using a TBI-on-a-chip model.
- Author
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Rogers, Edmond A., Beauclair, Timothy, Martinez, Jhon, Mufti, Shatha J., Kim, David, Sun, Siyuan, Stingel, Rachel L., Dieterly, Alexandra M., Krishnan, Nikita, Crodian, Jennifer, and Shi, Riyi
- Subjects
- *
NEURAL circuitry , *ACROLEIN , *AMYLOID beta-protein , *BRAIN injuries , *AMYLOID , *ALZHEIMER'S disease , *OXIDATIVE stress - Abstract
Trauma-induced Alzheimer's disease (AD) is rapidly emerging as a major consequence of traumatic brain injuries (TBI), with devastating social and economic impacts. Unfortunately, few treatment options are currently available due to a limited understanding of the underlying mechanisms. A clinically-relevant, in vitro experimental model that emulates in vivo scenarios with high levels of spatial and temporal resolution is critical for demystifying the pathways of post-TBI AD. Using a unique, recently established "TBI-on-a-chip" system with murine cortical networks, we demonstrate the correlative elevation of oxidative stress (acrolein), inflammation (TNF-α), and Aβ42 aggregation, with concomitant reduction of neuronal network electrical activity post-concussive impact. These findings confirm that TBI-on-a-chip could provide a novel paradigm to supplement in vivo studies of trauma, while simultaneously validating the interaction of these alleged, key-pathological factors in post-TBI AD development. Specifically, we have shown that acrolein, acting as a diffusive factor of secondary injury, is both critical and sufficient in promoting inflammation (TNF-α) and Aβ42 aggregation, two known contributors of AD pathogenesis. Furthermore, using a cell-free preparation with TBI-on-a-chip, we have confirmed that both force and acrolein can independently and directly stimulate the aggregation of purified Aβ42, highlighting the key capabilities of primary and secondary injury mechanisms towards inducing Aβ42 aggregation, independently and synergistically. In addition to morphological and biochemical assessment, we also demonstrate parallel monitoring of neuronal network activity, further validating the chief pathological role of acrolein in not only inflicting biochemical abnormalities, but also functional deficits in neuronal networks. In conclusion, through this line of investigations, we have shown that by recapitulating clinically-relevant events, the TBI-on-a-chip device is capable of quantitatively characterizing parallel force-dependent increases in oxidative stress, inflammation, protein aggregation, and network activity, offering a unique platform for mechanistic investigations of post-TBI AD, and trauma-induced neuronal injury in general. It is expected that this model could provide crucial insights into pathological mechanisms which will be critical in developing novel, effective diagnostics and treatment strategies that significantly benefit TBI victims. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
30. Semi-grand canonical Monte Carlo simulation of the acrolein induced surface segregation and aggregation of AgPd with machine learning surrogate models.
- Author
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Liu, Mingjie, Yang, Yilin, and Kitchin, John R.
- Subjects
- *
SURFACE segregation , *MONTE Carlo method , *MACHINE learning , *ACROLEIN , *WNT proteins , *SURFACE reactions - Abstract
The single atom alloy of AgPd has been found to be a promising catalyst for the selective hydrogenation of acrolein. It is also known that the formation of Pd islands on the surface will greatly reduce the selectivity of the reaction. As a result, the surface segregation and aggregation of Pd on the AgPd surface under reaction conditions of selective hydrogenation of acrolein are of great interest. In this work, we lay out a workflow that can predict the surface segregation and aggregation of Pd on a FCC(111) AgPd surface with and without the presence of acrolein. We use machine learning surrogate models to predict the AgPd bulk energy, AgPd slab energy, and acrolein adsorption energy on AgPd slabs. Then, we use the semi-grand canonical Monte Carlo simulation to predict the surface segregation and aggregation under different bulk Pd concentrations. Under vacuum conditions, our method predicts that only trace amount of Pd will exist on the surface at Pd bulk concentrations less than 20%. However, with the presence of acrolein, Pd will start to aggregate as dimers on the surface at Pd bulk concentrations as low as 6.5%. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
31. Acrolein produced during acute kidney injury promotes tubular cell death.
- Author
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Aihara, Seishi, Torisu, Kumiko, Hirashima, Yutaro, Kitazono, Takanari, and Nakano, Toshiaki
- Subjects
- *
ACUTE kidney failure , *POLYAMINES , *CELL death , *ACROLEIN , *KIDNEY tubules , *REPERFUSION injury - Abstract
Acute kidney injury is an important global health concern as it is associated with high morbidity and mortality. Polyamines, essential for cell growth and proliferation, are known to inhibit cardiovascular disease. However, under conditions of cellular damage, toxic acrolein is produced from polyamines by the enzyme spermine oxidase (SMOX). We used a mouse renal ischemia-reperfusion model and human proximal tubule cells (HK-2) to investigate whether acrolein exacerbates acute kidney injury by renal tubular cell death. Acrolein visualized by acroleinRED was increased in ischemia-reperfusion kidneys, particularly in tubular cells. When HK-2 cells were cultured under 1% oxygen for 24 h, then switched to 21% oxygen for 24 h (hypoxia-reoxygenation), acrolein accumulated and SMOX mRNA and protein levels were increased. Acrolein induced cell death and fibrosis-related TGFB1 mRNA in HK-2 cells. Administration of the acrolein scavenger cysteamine suppressed the acrolein-induced upregulation of TGFB1 mRNA. Cysteamine also inhibited a decrease in the mitochondrial membrane potential observed by MitoTrackerCMXRos, and cell death induced by hypoxia-reoxygenation. The siRNA-mediated knockdown of SMOX also suppressed hypoxia-reoxygenation-induced acrolein accumulation and cell death. Our study suggests that acrolein exacerbates acute kidney injury by promoting tubular cell death during ischemia-reperfusion injury. Treatment to control the accumulation of acrolein might be an effective therapeutic option for renal ischemia-reperfusion injury. • Acrolein accumulates in ischemia-reperfusion injured kidneys, especially in the renal tubules. • Acrolein induces tubular cell death. • Acrolein-induced cell death is inhibited by acrolein scavengers and spermine oxidase knockdown. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
32. Synthesis, Characterization, and DFT Calculations of a New Sulfamethoxazole Schiff Base and Its Metal Complexes.
- Author
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Al-Hawarin, Jibril I., Abu-Yamin, Abdel-Aziz, Abu-Saleh, Abd Al-Aziz A., Saraireh, Ibrahim A. M., Almatarneh, Mansour H., Hasan, Mahmood, Atrooz, Omar M., and Al-Douri, Y.
- Subjects
- *
SCHIFF bases , *TRANSITION metal complexes , *SULFAMETHOXAZOLE , *RING formation (Chemistry) , *RARE earth metals , *ISOXAZOLES , *METAL complexes - Abstract
A new Schiff base, 4-((1E,2E)-3-(furan-2-yl)allylidene)amino)-N-(5-methylisoxazol-3-yl) benzene-sulfonamide (L), was synthesized by thermal condensation of 3-(2-furyl)acrolein and sulfamethoxazole (SMX), and the furan Schiff base (L) was converted to a phenol Schiff base (L') according to the Diels–Alder [4 + 2] cycloaddition reaction and studied experimentally. The structural and spectroscopic properties of the Schiff base were also corroborated by utilizing density functional theory (DFT) calculations. Furthermore, a series of lanthanide and transition metal complexes of the Schiff base were synthesized from the nitrate salts of Gd, Sm, Nd, and Zn (L1, L2, L3, and L4), respectively. Various spectroscopic studies confirmed the chemical structures of the Schiff-base ligand and its complexes. Based on the spectral studies, a nine-coordinated geometry was assigned to the lanthanide complexes and a six-coordinated geometry to the zinc complex. The elemental analysis data confirmed the suggested structure of the metal complexes, and the TGA studies confirmed the presence of one coordinated water molecule in the lanthanide complexes and one crystalline water molecule in the zinc complex; in addition, the conductivity showed the neutral nature of the complexes. Therefore, it is suggested that the ligand acts as a bidentate through coordinates to each metal atom by the isoxazole nitrogen and oxygen atoms of the sulfur dioxide moiety of the SMX based on FTIR studies. The ligand and its complexes were tested for their anti-inflammatory, anti-hemolytic, and antioxidant activities by various colorimetric methods. These complexes were found to exhibit potential effects of the selected biological activities. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
33. Transient Receptor Potential Ankyrin 1 (TRPA1) Channel Mediates Acrolein Cytotoxicity in Human Lung Cancer Cells.
- Author
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Sakamoto, Akihiko, Terui, Yusuke, Igarashi, Kazuei, and Kashiwagi, Keiko
- Subjects
- *
ACROLEIN , *LUNG cancer , *CANCER cells , *CIGARETTE smoke , *SMOKING , *ION channels - Abstract
Transient receptor potential ankyrin 1 (TRPA1) is a nonselective ion channel implicated in thermosensation and inflammatory pain. It has been reported that expression of the TRPA1 channel is induced by cigarette smoke extract. Acrolein found in cigarette smoke is highly toxic and known as an agonist of the TRPA1 channel. However, the role of TRPA1 in the cytotoxicity of acrolein remains unclear. Here, we investigated whether the TRPA1 channel is involved in the cytotoxicity of acrolein in human lung cancer A549 cells. The IC50 of acrolein in A549 cells was 25 μM, and acrolein toxicity increased in a concentration- and time-dependent manner. When the effect of acrolein on TRPA1 expression was examined, the expression of TRPA1 in A549 cells was increased by treatment with 50 μM acrolein for 24 h or 500 μM acrolein for 30 min. AP-1, a transcription factor, was activated in the cells treated with 50 μM acrolein for 24 h, while induction of NF-κB and HIF-1α was observed in the cells treated with 500 μM acrolein for 30 min. These results suggest that acrolein induces TRPA1 expression by activating these transcription factors. Overexpression of TRPA1 in A549 cells increased acrolein sensitivity and the level of protein-conjugated acrolein (PC-Acro), while knockdown of TRPA1 in A549 cells or treatment with a TRPA1 antagonist caused tolerance to acrolein. These findings suggest that acrolein induces the TRPA1 channel and that an increase in TRPA1 expression promotes the cytotoxicity of acrolein. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
34. Unveiling Cutting Edge Innovations in the Catalytic Valorization of Biodiesel Byproduct Glycerol into Value Added Products.
- Author
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Mohan, Kavya, Pai, Sunaja Devi Kalathiparambil Rajendra, Reghunath, Baby Shalini, and Pinheiro, Dephan
- Subjects
- *
GLYCERIN , *CATALYST selectivity , *LACTIC acid , *ACROLEIN - Abstract
The increasing production of biodiesel has led to a glut in the production of glycerol, which is a byproduct. This has resulted in the quest for alternative applications using glycerol as a cheap and readily available starting material. One promising approach is the catalytic valorization of glycerol, which converts glycerol into valuable chemicals such as 1,2‐propanediol, lactic acid, and acrolein. The glycerol formed affects the efficiency of the biodiesel, and hence it must be removed. Different processes can convert glycerol to various useful products like glycerol carbonate, glycidol, solketal, lactic acid, and glyceric acid. These different products, the processes used for synthesis, and the various catalysts used have been discussed. The most effective methods for the syntheses, the numerous catalyst systems, mechanisms of the reactions, and applications of these products in different fields are discussed in this review. The paper also discusses the challenges and opportunities of glycerol valorization, including the need for improved catalyst selectivity and activity and the potential for integrating glycerol valorization with other biorefinery processes. Overall, the catalytic valorization of glycerol offers a promising pathway for utilizing this abundantly available resource, and this review provides valuable insights for researchers and practitioners working in this area. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
35. The role of acrolein for E-cigarette vapour condensate mediated activation of NADPH oxidase in cultured endothelial cells and macrophages.
- Author
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Kuntic, Ivana, Kuntic, Marin, Oelze, Matthias, Stamm, Paul, Karpi, Angelica, Kleinert, Hartmut, Hahad, Omar, Münzel, Thomas, and Daiber, Andreas
- Subjects
- *
NICOTINAMIDE adenine dinucleotide phosphate , *NUCLEAR factor E2 related factor , *NADPH oxidase , *ELECTRONIC cigarettes , *ENDOTHELIAL cells , *ACROLEIN - Abstract
Electronic cigarettes (E-cigarettes) have recently become a popular alternative to traditional tobacco cigarettes. Despite being marketed as a healthier alternative, increasing evidence shows that E-cigarette vapour could cause adverse health effects. It has been postulated that degradation products of E-cigarette liquid, mainly reactive aldehydes, are responsible for those effects. Previously, we have demonstrated that E-cigarette vapour exposure causes oxidative stress, inflammation, apoptosis, endothelial dysfunction and hypertension by activating NADPH oxidase in a mouse model. To better understand oxidative stress mechanisms, we have exposed cultured endothelial cells and macrophages to condensed E-cigarette vapour (E-cigarette condensate) and acrolein. In both endothelial cells (EA.hy 926) and macrophages (RAW 264.7), we have observed that E-cigarette condensate incubation causes cell death. Since recent studies have shown that among toxic aldehydes found in E-cigarette vapour, acrolein plays a prominent role, we have incubated the same cell lines with increasing concentrations of acrolein. Upon incubation with acrolein, a translocation of Rac1 to the plasma membrane has been observed, accompanied by an increase in oxidative stress. Whereas reactive oxygen species (ROS) formation by acrolein in cultured endothelial cells was mainly intracellular, the release of ROS in cultured macrophages was both intra- and extracellular. Our data also demonstrate that acrolein activates the nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant pathway and, in general, could mediate E-cigarette vapour-induced oxidative stress and cell death. More mechanistic insight is needed to clarify the toxicity associated with E-cigarette consumption and the possible adverse effects on human health. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
36. Catalytic Properties of Rh-Containing Carbon Dots on SiO2, Al2O3, and ZrO2 Oxide Supports in the Glycerol Conversion.
- Author
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Kosmambetova, G. R., Vlasenko, N. V., Kharkova, L. B., Yanko, O. H., Ogenko, V. M., Grytsenko, V. I., and Shvets, O. V.
- Subjects
- *
ALUMINUM oxide , *ACETALDEHYDE , *OXIDES , *GLYCERIN , *CHEMICAL yield , *CARBON , *CATALYTIC activity - Abstract
It is shown that deposition of Rh-CD (CDs are carbon dots)onto SiO2, Al2O3, and ZrO2 oxide supports contributes to the appearance of the catalytic activity in the process of glycerol conversion. It is established that acrolein and acetaldehyde are oxygen-containing products in this reaction system. It is found that the yield of reaction products increases when using a support with a smaller specific surface area, which is caused by an increase in the surface concentration of acid sites. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
37. Stereoselective Synthesis of Contiguous Cyclopropylaziridines: Enabling the Construction of Versatile Chiral Molecules Including γ‐Lactams via Ring‐Opening Reactions.
- Author
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Han, Jiho, Ha, Hyun‐Joon, and Yang, Jung Woon
- Subjects
- *
RING-opening reactions , *CYCLOPROPANATION , *MOLECULES , *LACTAMS , *ACROLEIN , *PYRROLIDINE - Abstract
The synthesis of chiral contiguous cyclopropylaziridine was achieved through cyclopropanation of (E)‐3‐((S)‐1‐[(R)‐1‐phenylethyl)aziridin‐2‐yl]acrylaldehyde with bromomalonate in the presence of (2R)‐ or (2S)‐[diphenyl(trimethylsilyloxy) methyl]pyrrolidine as a chiral organocatalyst and 2,6‐lutidine as a base. This chiral cyclopropylaziridine was then subjected to various functional transformations, leading to the construction of a wide range of structurally important motifs. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
38. Acrolein adducts and responding autoantibodies correlate with metabolic disturbance in Alzheimer's disease.
- Author
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Sanotra, Monika Renuka, Kao, Shu-Huei, Lee, Ching-Kuo, Hsu, Chun-Hsien, Huang, Wen-Chung, Chang, Tsuei-Chuan, Tu, Fang-Yu, Hsu, I-Uen, and Lin, Yung-Feng
- Subjects
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ALZHEIMER'S disease , *IMMUNOGLOBULIN M , *METABOLIC disorders , *ACROLEIN , *AMYLOID beta-protein precursor , *AUTOANTIBODIES - Abstract
Background: Alzheimer's disease (AD) is caused by many intertwining pathologies involving metabolic aberrations. Patients with metabolic syndrome (MetS) generally show hyperglycemia and dyslipidemia, which can lead to the formation of aldehydic adducts such as acrolein on peptides in the brain and blood. However, the pathogenesis from MetS to AD remains elusive. Methods: An AD cell model expressing Swedish and Indiana amyloid precursor protein (APP-Swe/Ind) in neuro-2a cells and a 3xTg-AD mouse model were used. Human serum samples (142 control and 117 AD) and related clinical data were collected. Due to the involvement of MetS in AD, human samples were grouped into healthy control (HC), MetS-like, AD with normal metabolism (AD-N), and AD with metabolic disturbance (AD-M). APP, amyloid-beta (Aß), and acrolein adducts in the samples were analyzed using immunofluorescent microscopy, histochemistry, immunoprecipitation, immunoblotting, and/or ELISA. Synthetic Aß1-16 and Aß17-28 peptides were modified with acrolein in vitro and verified using LC–MS/MS. Native and acrolein-modified Aß peptides were used to measure the levels of specific autoantibodies IgG and IgM in the serum. The correlations and diagnostic power of potential biomarkers were evaluated. Results: An increased level of acrolein adducts was detected in the AD model cells. Furthermore, acrolein adducts were observed on APP C-terminal fragments (APP-CTFs) containing Aß in 3xTg-AD mouse serum, brain lysates, and human serum. The level of acrolein adducts was correlated positively with fasting glucose and triglycerides and negatively with high-density lipoprotein-cholesterol, which correspond with MetS conditions. Among the four groups of human samples, the level of acrolein adducts was largely increased only in AD-M compared to all other groups. Notably, anti-acrolein-Aß autoantibodies, especially IgM, were largely reduced in AD-M compared to the MetS group, suggesting that the specific antibodies against acrolein adducts may be depleted during pathogenesis from MetS to AD. Conclusions: Metabolic disturbance may induce acrolein adduction, however, neutralized by responding autoantibodies. AD may be developed from MetS when these autoantibodies are depleted. Acrolein adducts and the responding autoantibodies may be potential biomarkers for not only diagnosis but also immunotherapy of AD, especially in complication with MetS. [ABSTRACT FROM AUTHOR]
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- 2023
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39. Synthesis of Novel Ionic Porous Organic Polymers and Its Application in Hydroxyl Condensation Reaction.
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Wang, Yajuan, Liu, Wenqiang, Zhang, Juan, and Shan, Qingwen
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POLYMERS , *POROUS polymers , *CONDENSATION reactions , *FOURIER transform infrared spectroscopy , *HETEROGENEOUS catalysts , *CATALYTIC activity , *PROPIONALDEHYDE , *ACTIVATION energy - Abstract
A novel ionic porous organic polymers catalysts were prepared and characterized by scanning electron microscopy, Brunner − Emmet − Teller measurements, fourier transform infrared spectroscopy and thermogravimetric analysis The characterization results showed that the synthesized catalyst was a porous polymer and had good heat stability. The catalytic performance of the catalyst was evaluated by synthesis of methacrolein from formaldehyde and propionaldehyde. The optimal reaction conditions were: the catalyst amount was 10%(wt%)of propionic aldehyde mass, the reaction temperature was 85 ℃, the reaction time was 1.5 h, the ratio of propionaldehyde and formaldehyde was 1:1.2. Under optimal condition, the conversion rate of propionaldehyde was up to 99.57% and the selectivity of methacrolein was 98.77%. The catalytic activity did not reduced obviously after being reused for 5 times. The reaction kinetics of synthesis of methacrolein was further studied. The results showed that the reaction order was 1.6, the activation energy was 50.28 kJ/mol and the pre-exponential factor was 4.17 × 107 (mol/L)−1.456/s. [ABSTRACT FROM AUTHOR]
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- 2023
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40. Sex-specific respiratory and systemic endocrine effects of acute acrolein and trichloroethylene inhalation.
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Alewel, Devin I., Jackson, Thomas W., Vance, Samuel A., Schladweiler, Mette C., Evansky, Paul A., Henriquez, Andres R., Grindstaff, Rachel, Gavett, Stephen H., and Kodavanti, Urmila P.
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ENDOCRINE glands , *ACROLEIN , *AIR pollutants , *TRICHLOROETHYLENE , *POLLUTANTS - Abstract
Acrolein and trichloroethylene (TCE) are priority hazardous air pollutants due to environmental prevalence and adverse health effects; however, neuroendocrine stress-related systemic effects are not characterized. Comparing acrolein, an airway irritant, and TCE with low irritancy, we hypothesized that airway injury would be linked to neuroendocrine-mediated systemic alterations. Male and female Wistar-Kyoto rats were exposed nose-only to air, acrolein or TCE in incremental concentrations over 30 min, followed by 3.5-hr exposure to the highest concentration (acrolein - 0.0, 0.1, 0.316, 1, 3.16 ppm; TCE - 0.0, 3.16, 10, 31.6, 100 ppm). Real-time head-out plethysmography revealed acrolein decreased minute volume and increased inspiratory-time (males>females), while TCE reduced tidal-volume. Acrolein, but not TCE, inhalation increased nasal-lavage-fluid protein, lactate-dehydrogenase activity, and inflammatory cell influx (males>females). Neither acrolein nor TCE increased bronchoalveolar-lavage-fluid injury markers, although macrophages and neutrophils increased in acrolein-exposed males and females. Systemic neuroendocrine stress response assessment indicated acrolein, but not TCE, increased circulating adrenocorticotrophic hormone, and consequently corticosterone, and caused lymphopenia, but only in males. Acrolein also reduced circulating thyroid-stimulating hormone, prolactin, and testosterone in males. In conclusion, acute acrolein inhalation resulted in sex-specific upper respiratory irritation/inflammation and systemic neuroendocrine alterations linked to hypothalamic-pituitary-adrenal axes activation, which is critical in mediating extra-respiratory effects. • Acrolein but not TCE elicits acute respiratory irritancy and systemic stress. • Acrolein-induced respiratory injury prompts sex-specific SAM/HPA axes activation. • Acute acrolein inhalation increases HPA and decreases HPT and HPG neurohormones. • Integrating neuroendocrine assessment could inform etiology of HAPs stress effects. [ABSTRACT FROM AUTHOR]
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- 2023
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41. Continuous dehydration of glycerol to Acrolein based on HPW/TS-COOH catalyzation.
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Su, Chen-Xin, Chen, Jing-Jing, Wu, Shao-Yun, Li, Shao-Heng, Zhou, Shou-Quan, Zheng, Hui-Dong, and Zhang, Fu-Weng
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GLYCERIN , *ACROLEIN , *CONTINUOUS flow reactors , *DEHYDRATION , *BRONSTED acids , *CATALYST synthesis - Abstract
Sustainable synthesis of acrolein, a key chemical intermediate, from biomass-derived glycerol is highly attractive. However, conventional catalysts for the dehydration of glycerol suffer from low acrolein selectivity and high deactivation tendency. Herein, a novel green catalyst (HPW/T0.6 S-COOH) was prepared and employed in the dehydration of glycerol in a continuous flow reactor. The performance of different catalysts and the effects of reaction conditions (reaction temperature, N2 flow rate, and glycerol concentration) were examined. The HPW/T0.6 S-COOH catalyst provides the best glycerol conversion of 96.38% and acrolein selectivity of 92.01%. The NH3-TPD and pyridine-FTIR results indicate that the Brønsted acid site is more susceptible to acrolein, while the weak strength acid site effectively prevents the further reaction of acrolein, providing practical insights for the rational design of efficient and continuous synthesis of acrolein catalysts. Research highlights: A novel green catalyst HPW/T0.6 S-COOH was synthesized for the dehydration of glycerol. The dehydration in a micro bed packed with the novel catalyst achieves high glycerol conversion and acrolein selectivity of 96.38% and 92.01%, respectively. Weak Brønsted acid site is proved to contribute to the high selectivity to acrolein. [ABSTRACT FROM AUTHOR]
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- 2023
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42. Mechanism and Kinetic Study on Synthesis of Methacrolein Catalyzed by Amine/Acid.
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Wang, Tao, Li, Jie, Yan, Hanwen, Zhang, Guoliang, and Li, Shifeng
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MANNICH bases , *AMINES , *STERIC hindrance , *SECONDARY amines , *ACTIVATION energy , *FORMALDEHYDE , *BORONIC acids , *POLYMERS - Abstract
Methacrolein (MAL) is an important intermediate extensively used in the manufacture of methyl methacrylate and other materials (polymers and resins). In this study, a series of secondary amines/acids were explored as catalysts for the condensation of formaldehyde and propionaldehyde to prepare MAL. It was found that the structure of the amines and acids directly affected the yield of MAL. The effect of the catalyst was closely related to the nucleophilicity of the amines as well as the steric hindrance effect, while acids also played a role as co-catalysts. Dibutylamine acetate was selected as the catalyst after investigation. The catalytic performance of the system was systematically studied by a series of single-factor experiments, including stirring rate, temperature, reaction time, acid/amine ratio, and the solvent, and the optimized reaction conditions were obtained. In the optimum condition, the yield of MAL was up to 97.3%. Kinetic experiments were performed for the condensation of formaldehyde and propionaldehyde to MAL, and the activation energies, reaction orders, and rate-limiting step of the reaction were determined. The results indicate that the decomposition of the Mannich base is a rate-limiting step. [ABSTRACT FROM AUTHOR]
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- 2023
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43. ПРИГОТОВЛЕНИЕ ЭКОЛОГИЧЕСКИ ЧИСТОЙ СЕРОБЕТОННОЙ СМЕСИ НА ОСНОВЕ ПОЛИМЕРНОЙ СЕРЫ
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ХУРСАНДОВ, Б. Ш., ЮСУПОВ, Ф. М., and КУЧАРОВ, А. А.
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SULFUR , *FUNCTIONAL groups , *POLYMER-impregnated concrete , *PETROLEUM industry , *CORROSION resistance , *CONCRETE , *POLYMERS - Abstract
Background. It is important to obtain sulfur concrete by modifying technical sulfur obtained as a result of oil and gas processing in the territory of Uzbekistan. Purpose. Production of sulfur concrete based on synthesized polymer sulfur and analysis of its physical and mechanical properties. Methodology. IR spectroscopy was used to determine the functional groups in polymer sulfur. The change in concrete thickness and mechanical strength at a temperature of 80 °C was determined based on ПНСТ105-2016. Originality. Optimum conditions for obtaining polymer sulfur from technical sulfur were determined and its structure was explained by spectroscopic methods. The optimal amount of polymer sulfur required for the preparation of sulfur concrete mixture has been scientifically proven. Findings. Samples of sulfur concrete were prepared based on synthesized polymer sulfur. The change in the mechanical strength of sulfur concrete samples depending on the storage period has been scientifically analyzed. [ABSTRACT FROM AUTHOR]
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- 2023
44. Effects of C-Terminal-Ethyl-Esterification in a Snake-Venom-Based Peptide Against the Neurotoxicity of Acrolein in PC12 Cells.
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Bernardes, Carolina P., Santos, Neife A. G., Costa, Tassia R., Menaldo, Danilo L., Sisti, Flavia M., Amstalden, Martin K., Ribeiro, Diego L., Antunes, Lusânia M. G., Sampaio, Suely V., and Santos, Antonio C.
- Abstract
We have recently reported the neurotrophic and neuroprotective effects of the snake-venom-based synthetic tripeptide BTX-I in PC12 cells treated with acrolein. In the present study, this peptide was chemically modified to increase its neurotrophic/neuroprotective activity. Esterification (ethyl or methyl), PEGylation and amidation were introduced at the C-terminus; acetylation was introduced at the N-terminus. The modified peptides protected PC12 cells from the decrease in viability and neuritogenesis induced by acrolein; however, only the ethyl-esterified peptide (named BTX-II) significantly increased neuritogenesis in comparison with the original peptide BTX-I. Moreover, BTX-II increased the expression of proteins related to (i) axonal/synaptic plasticity (synapsin I, β-III-Tubulin), and (ii) energy metabolism (AMPK-α and SIRT I) in PC12 cells treated with acrolein. In addition, BTX-II upregulated the expression of genes that encode apolipoprotein E (ApoE) and Mitogen-Activated Protein Kinase 3 (Mapk3), which are associated with cognitive improvements in animal models of Alzheimer’s disease (AD). In conclusion, ethyl-esterification at the C-terminus of the snake-venom-based tripeptide [Glu-Val-Trp] improved the neurotrophic and neuroprotective potential in relation to the original tripeptide. [ABSTRACT FROM AUTHOR]
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- 2023
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45. Liquid-Phase Dehydration of Glycerol to Acrolein with ZSM-5-Based Catalysts in the Presence of a Dispersing Agent.
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Huang, Lin, Wang, Bo, Liu, Licheng, and Borgna, Armando
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DISPERSING agents , *ACROLEIN , *ACID catalysts , *BRONSTED acids , *CATALYSTS , *DEHYDRATION , *GLYCERIN - Abstract
Liquid-phase dehydration of glycerol to acrolein was investigated with solid acid catalysts, including H-ZSM-5, H3PO4-modified H-ZSM-5, H3PW12O40·14H2O and Cs2.5H0.5PW12O40, in the presence of sulfolane ((CH2)4SO2) as a dispersing agent under atmospheric pressure N2 in a batch reactor. High weak-acidity H-ZSM-5, high temperatures and high-boiling-point sulfolane improved the activity and selectivity for the production of acrolein through suppressing the formation of polymers and coke and promoting the diffusion of glycerol and products. Brønsted acid sites were soundly demonstrated to be responsible for dehydration of glycerol to acrolein by infrared spectroscopy of pyridine adsorption. Brønsted weak acid sites favored the selectivity to acrolein. Combined catalytic and temperature-programmed desorption of ammonia studies revealed that the selectivity to acrolein increased as the weak-acidity increased over the ZSM-5-based catalysts. The ZSM-5-based catalysts produced a higher selectivity to acrolein, while the heteropolyacids resulted in a higher selectivity to polymers and coke. [ABSTRACT FROM AUTHOR]
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- 2023
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46. Diet as a Source of Acrolein: Molecular Basis of Aldehyde Biological Activity in Diabetes and Digestive System Diseases.
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Hikisz, Pawel and Jacenik, Damian
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DIGESTIVE system diseases , *ACROLEIN , *TOBACCO smoke , *ALDEHYDES , *INTESTINAL cancer , *PUBLIC health - Abstract
Acrolein, a highly reactive α,β-unsaturated aldehyde, is a compound involved in the pathogenesis of many diseases, including neurodegenerative diseases, cardiovascular and respiratory diseases, diabetes mellitus, and the development of cancers of various origins. In addition to environmental pollution (e.g., from car exhaust fumes) and tobacco smoke, a serious source of acrolein is our daily diet and improper thermal processing of animal and vegetable fats, carbohydrates, and amino acids. Dietary intake is one of the main routes of human exposure to acrolein, which is a major public health concern. This review focuses on the molecular mechanisms of acrolein activity in the context of its involvement in the pathogenesis of diseases related to the digestive system, including diabetes, alcoholic liver disease, and intestinal cancer. [ABSTRACT FROM AUTHOR]
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- 2023
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47. Sensitization of GSH synthesis by curcumin curtails acrolein-induced alveolar epithelial apoptosis via Keap1 cysteine conjugation: A randomized controlled trial and experimental animal model of pneumonitis.
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Hee Jo, Eun, Eun Moon, Ji, Han Chang, Moon, Jin Lim, Ye, Hyun Park, Jung, Hee Lee, Suk, Rae Cho, Young, Cho, Art E, Pil Pack, Seung, Kim, Hyeon-Wee, Crowley, Liana, Le, Brandy, Nukhet, Aykin-Burns, Chen, Yinfeng, Zhong, Yihang, Zhao, Jiangchao, Li, Ying, Cha, Hanvit, Hoon Pan, Jeong, and Kyeom Kim, Jae
- Subjects
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AIR pollutants , *CURCUMIN , *RANDOMIZED controlled trials , *LABORATORY animals , *PNEUMONIA , *CHRONIC obstructive pulmonary disease , *CYSTEINE - Abstract
[Display omitted] • Curcumin improves systemic inflammation in subjects residing in an air-polluted area. • Curcumin mitigates acrolein-induced pneumonitis in a preclinical animal model. • Curcumin inhibits acrolein-induced alveolar epithelial apoptosis in a ROS-dependent manner. • Curcumin increases redox sensing of Nrf2 and thus sensitizes de novo GSH synthesis. • Curcumin-Keap1 cysteine conjugation enhances redox sensing of Nrf2. Epidemiological studies have reported an association between exposures to ambient air pollution and respiratory diseases, including chronic obstructive pulmonary disease (COPD). Pneumonitis is a critical driving factor of COPD and exposure to air pollutants (e.g., acrolein) is associated with increased incidence of pneumonitis. Currently available anti-inflammatory therapies provide little benefit against respiratory diseases. To this end, we investigated the preventive role of curcumin against air pollutant-associated pneumonitis and its underlying mechanism. A total of 40 subjects was recruited from Chengdu, China which is among the top three cities in terms of respiratory mortality related to air pollution. The participants were randomly provided either placebo or curcumin supplements for 2 weeks and blood samples were collected at the baseline and at the end of the intervention to monitor systemic markers. In our follow up mechanistic study, C57BL/6 mice (n = 40) were randomly allocated into 4 groups: Control group (saline + no acrolein), Curcumin only group (curcumin + no acrolein), Acrolein only group (saline + acrolein), and Acrolein + Curcumin group (curcumin + acrolein). Curcumin was orally administered at 100 mg/kg body weight once a day for 10 days, and then the mice were subjected to nasal instillation of acrolein (5 mg/kg body weight). Twelve hours after single acrolein exposure, all mice were euthanized. Curcumin supplementation, with no noticeable adverse responses, reduced circulating pro-inflammatory cytokines in association with clinical pneumonitis as positive predictive while improving those of anti-inflammatory cytokines. In the pre-clinical study, curcumin reduced pneumonitis manifestations by suppression of intrinsic and extrinsic apoptotic signaling, which is attributed to enhanced redox sensing of Nrf2 and thus sensitized synthesis and restoration of GSH, at least in part, through curcumin-Keap1 conjugation. Our study collectively suggests that curcumin could provide an effective preventive measure against air pollutant-enhanced pneumonitis and thus COPD. [ABSTRACT FROM AUTHOR]
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- 2023
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48. Impact of sub-acute acrolein inhalation on the molecular regulation of mitochondrial metabolism in rat lung.
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Tulen, C.B.M., Leermakers, P.A., Schrieder, S.E., van Schooten, F.J., Opperhuizen, A., and Remels, A.H.V.
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METABOLIC regulation , *HOMEOSTASIS , *ACROLEIN , *METABOLISM - Abstract
Nowadays, mitochondria are recognized as key players in the pathogenesis of a variety of smoking-associated lung diseases. Acrolein, a component of cigarette smoke, is a known driver of biological mechanisms underlying smoking-induced respiratory toxicity. The impact of sub-acute acrolein inhalation in vivo on key processes controlling mitochondrial homeostasis in cells of the airways however is unknown. In this study, we investigated the activity/abundance of a myriad of molecules critically involved in mitochondrial metabolic pathways and mitochondrial quality control processes (mitochondrial biogenesis and mitophagy) in the lungs of Sprague-Dawley rats that were sub-acutely exposed to filtered air or 3 ppm acrolein by whole-body inhalation (5 h/day, 5 days/week for 4 weeks). Acrolein exposure induced a general inflammatory response in the lung as gene expression analysis revealed an increased expression of Icam1 and Cinc1 (p < 0.1; p < 0.05). Acrolein significantly decreased enzyme activity of hydroxyacyl-Coenzyme A dehydrogenase (p < 0.01), and decreased Pdk4 transcript levels (p < 0.05), suggestive of acrolein-induced changes in metabolic processes. Investigation of constituents of the mitochondrial biogenesis pathways and mitophagy machinery revealed no pronounced alterations. In conclusion, sub-acute inhalation of acrolein did not affect the regulation of mitochondrial metabolism and quality control, which is in contrast to more profound changes after acute exposure in other studies. [Display omitted] • Sub-acute acrolein exposure induces a general inflammatory response in rat lung. • Sub-acute acrolein inhalation does not impact mitochondrial metabolism in lung. • No alterations of mitochondrial quality control indices upon sub-acute acrolein inhalation. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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49. Effects of the Acidic and Textural Properties of Y-Type Zeolites on the Synthesis of Pyridine and 3-Picoline from Acrolein and Ammonia.
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Pala-Rosas, Israel, Contreras, José Luis, Salmones, José, López-Medina, Ricardo, Angeles-Beltrán, Deyanira, Zeifert, Beatriz, Navarrete-Bolaños, Juan, and González-Hernández, Naomi N.
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ACROLEIN , *ZEOLITES , *PYRIDINE , *CATALYTIC activity , *BRONSTED acids , *ACETALDEHYDE , *AMMONIA - Abstract
A set of Y-type zeolites with Si/Al atomic ratios between 7–45 were studied as catalysts in the aminocyclization reaction between acrolein and ammonia to produce pyridine and 3-picoline. The catalytic activity tests at 360 °C revealed that the acrolein conversion increased in the order Z45 < ZY34 < ZY7 < ZY17, in agreement with the increase of the total acidity per gram of catalyst. In all cases, pyridine bases and cracking products (acetaldehyde and formaldehyde) were detected in the outflow from the reactor. The total yield of pyridines was inversely proportional to the total acidity for the catalysts, which presented large surface areas and micro- and mesoporosity. The selectivity towards 3-picoline was favored when using catalysts with a Brønsted/Lewis acid sites ratio close to 1. The formation of pyridine occurred more selectively over Lewis acid sites than Brønsted acid sites. The deactivation tests showed that the time on stream of the catalysts depended on the textural properties of zeolites, i.e., large pore volume and large BET area, as evidenced by the deactivation rate constants and the characterization of the spent catalysts. The physicochemical properties of the catalysts were determined by XRD, UV-vis, and Raman spectroscopies, infrared spectroscopy with adsorbed pyridine, N2 physisorption, and SEM-EDXS. After the reaction, the spent catalysts were characterized by XRD, Raman spectroscopy, TGA, and SEM-EDXS, indicating that the uniform deposition of polyaromatic species on the catalyst surface and within the porous system resulted in the loss of activity. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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50. Correlation of the presence of acrolein with higher alcohols, glycerol, and acidity in cachaças.
- Author
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Alvarenga, Gabriela Fontes, de Resende Machado, Ana Maria, Barbosa, Richard Bispo, Ferreira, Vanuzia Rodrigues Fernandes, Santiago, Wilder Douglas, Teixeira, Maria Luiza, Nelson, David Lee, and Cardoso, Maria das Graças
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ACROLEIN , *GLYCERIN , *ETHANOL , *PRINCIPAL components analysis , *CHEMICAL decomposition , *ACIDITY - Abstract
Acrolein is a toxic aldehyde that can be present in various beverages, such as cachaça and other distilled spirits from sugarcane. The objective of this work was to detect and quantify acrolein in samples of cachaça produced by different processes in all regions of Brazil and to evaluate the possible routes of formation of this contaminant from the correlation with other secondary compounds present in the beverage using principal component analysis. Approximately 27.0% of the samples analyzed were outside the limit established by Brazilian legislation for this contaminant, with an average acrolein concentration of 14.01 mg 100 mL−1 anhydrous alcohol (aa). In the other samples, the average concentration was 0.97 mg 100 mL−1 aa. After selecting the variables that most closely correlated with the presence of acrolein in beverages, a positive correlation was found with the presence of butan‐2‐ol, propan‐1‐ol and volatile acids, and a slight correlation with the presence of phenolic compounds. Therefore, the presence of acrolein in cachaça can be associated with contamination of the fermentation must by bacteria of the genus Lactobacillus, as a result of the chemical degradation and enzymatic conversion of the glycerol produced during fermentation. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
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