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Abstract Background Heat shock protein 20 (HSP20) is a member of the heat stress-related protein family, which plays critical roles in plant growth, development, and response to abiotic stresses. Although many HSP20 genes have been associated with heat stress in numerous types of plants, little is known about the details of the HSP20 gene family in Coix. To investigate the mechanisms of the ClHSP20 response to heat and drought stresses, the ClHSP20 gene family in Coix was identified and characterized based on genome-wide analysis. Results A total of 32 putative ClHSP20 genes were identified and characterized in Coix. Phylogenetic analysis indicated that ClHSP20s were grouped into 11 subfamilies. The duplicated event analysis demonstrated that tandem duplication and segment duplication events played crucial roles in promoting the expansion of the ClHSP20 gene family. Synteny analysis showed that Coix shared the highest homology in 36 HSP20 gene pairs with wheat, followed by 22, 19, 15, and 15 homologous gene pairs with maize, sorghum, barley, and rice, respectively. The expression profile analysis showed that almost all ClHSP20 genes had different expression levels in at least one tissue. Furthermore, 22 of the 32 ClHSP20 genes responded to heat stress, with 11 ClHSP20 genes being significantly upregulated and 11 ClHSP20 genes being significantly downregulated. Furthermore, 13 of the 32 ClHSP20 genes responded to drought stress, with 6 ClHSP20 genes being significantly upregulated and 5 ClHSP20 genes being significantly downregulated. Conclusions Thirty-two ClHSP20 genes were identified and characterized in the genome of Coix. Tandem and segmental duplication were identified as having caused the expansion of the ClHSP20 gene family. The expression patterns of the ClHSP20 genes suggested that they play a critical role in growth, development, and response to heat and drought stress. The current study provides a theoretical basis for further research on ClHSP20s and will facilitate the functional characterization of ClHSP20 genes.

Flavonoids are the important components of bioactive substances in coix seeds, uncovering component differentiation and functional orientation is also the important content in quality evolution. In this study, eight coix cultivars were used as the test materials to quantitatively detect flavonoids and their derivatives by metabonomics method, and component differentiation was analyzed. The results showed that 17 flavoniod molecules in total were detected in the eight coix materials and the metabolome profiles were similar among the cultivars with same testa color. The yellow testa cultivars contained more flavonoids in abundance and content than the red testa cultivars. The four molecules of quercetin 3-O-glucoside, rutin, eriodictyol, and luteolin, and were the main characteristic components in coix seeds, comprising a ratio of 45.35%, 32.77%, 6.04% and 5.37%, which exhibited multiple physiological activities such as antioxidation, anti-inflammatory, anti-tumor, antibacterial and viral, metabolic regulation, and neuroprotection. Formation of the characteristics of yellow and red testa in coix seeds might be related to differentiation of flavoniod accumulation, the main characteristic components in coix seeds had the effects such as antioxidation, anti-inflammatory, so they could be excavated and exploited as functional components in coix seeds. [ABSTRACT FROM AUTHOR]

Coix is a minor cereal crop with various applications in Traditional Chinese Medicine. Coix seed is called "Green Food", because of its high unsaturated fatty acid content with minimal toxic residues and heavy metals. Coix can be an excellent choice as a dietary supplement because of diverse bioactive compounds including phenols, flavonoids, polysaccharides, fibers, lipids, proteins, vitamins, and minerals with significant therapeutic effects such as anticancer, antioxidant, anti-inflammatory, anti-allergic, and immunomodulatory, among others. The least attention has been given to the improvement of new elite cultivars of coix with high nutritional values and nutraceutical effects. To date, very few genomic studies, in particular, draft genome and de novo sequencing have been conducted to discover the genes responsible for nutritional traits and their molecular mechanisms that are insufficient for the efficient use of existing germplasms for molecular breeding of coix. Therefore, this review article attempts to highlight bioactive compounds identified from coix with their medicinal uses as well as the recent progress in coix genomic studies which will create research scope for the development of nutrient-rich coix varieties with high therapeutic values. [ABSTRACT FROM AUTHOR]

Phytochemical investigation of the seeds of Coix lacryma-jobi var. lacryma-jobi resulted in the isolation of eight compounds, including two new structures, coixdines A-B (1-2) and six known compounds (3-8). The structures of these compounds were determined by 1 D and 2 D NMR spectra referring to the literatures, together with HR-MS analysis. Coixdine A and B are first examples of spermidine skeleton in genus Coix. In the present paper, all compounds were evaluated for the cytotoxicity against two cancer cell lines (CT-26 and BxPC-3) in vitro, and none of the compounds exhibited obvious cytotoxic activity. The present investigation suggests that these amides seem to be of great chemotaxonomic value for C. lacryma-jobi. [ABSTRACT FROM AUTHOR]

Coix (Coix lacryma-jobi) comes under underutilized crops as it is neither cultivated commercially on a large scale nor widely traded. The Northeastern region of India harbours a rich diversity of Coix. This crop has diverse varieties from food to ethnic ornaments worn by the Mizo people. The soft-shelled type (var ma-yuen), locally called 'Mim' has been an important cereal crop among the Mizo rural communities for a long time. The grains are highly nutritious and its exceptional medicinal aspects have been extensively researched. Meanwhile, the seeds of the wild type (var lacryma-jobi) are excellent beads for ornamental purposes and are key components in making traditional head gear 'Vakiria' worn by Mizo ladies on festive occasions. It is recently gaining importance as a 'functional food', because it is a rich source of nutrients and bioactive compounds. Its grains are gluten free which benefits people with celiac disease. It is used for preparing flour, soups, porridge, gruels, salads, desserts, snack, wine, sweets, etc. It is found to have diuretic, anti-inflammatory, antidiarrheal, antioxidant, antipyretic, antiseptic, antispasmodic, analgesic, hypotensive, and sedative properties. Coix germplasm are rapidly disappearing from wild and jhum lands, and without conservation a large population of genetic diversity will be lost from Northeast India. Mizoram also possesses a wide array of germplasm which could potentially contribute to research priorities. However, the ignorance on nutritional value of the crop, lack of standard agronomic practice, unavailability of high yielding quality seeds, potential varieties suitable for Mizoram conditions, processing infrastructures and marketing have diminished the interest of farmers in popularizing this crop. In this regards, thirty-nine Coix genotypes were evaluated owing to their agronomic characteristics to identify promising genotypes suitable for Mizoram conditions. Based on the overall mean performances for yield and yield components, two genotypes RJT-1 (39.74 q/ha) and RJT-2 (35.27 q/ha) were found superior for seed yield and other yield-related traits under Mizoram conditions. In addition, the occurrence of invasive fall armyworm, Spodoptera frugiperda (J.E. Smith) on Coix was observed and the damage incidence ranged between 7.23 and 10.86% in the vegetative growth stages (45–75 days crop) from the last week of August to the end of September. To the best of our knowledge, this is the first report of fall armyworm occurrence on Coix crop from India. [ABSTRACT FROM AUTHOR]

Auxin response factor (ARF) is an important transcription factor that regulates the expression of auxin-responsive genes by direct binding to their promoters, which play a central role in plant growth, development, and response to abiotic stresses. The availability of the entire Coix (Coix lacryma-jobi L.) genome sequence provides an opportunity to investigate the characteristics and evolutionary history of the ARF gene family in this medicine and food homology plant for the first time. In this study, a total of 27 ClARF genes were identified based on the genome-wide sequence of Coix. Twenty-four of the 27 ClARF genes were unevenly distributed on 8 chromosomes except Chr 4 and 10, and the remaining three genes (ClARF25-27) were not assigned to any chromosome. Most of the ClARF proteins were predicted to be localized to the nucleus, except ClARF24, which was localized to both the plasma membrane and nucleus. Twenty-seven ClARFs were clustered into six subgroups based on the phylogenetic analysis. Duplication analysis showed that segmental duplication, rather than tandem duplications promoting the expansion of the ClARF gene family. Synteny analysis showed that purifying selection might have been a primary driving force in the development of the ARF gene family in Coix and other investigated cereal plants. The prediction of the cis element of the promoter showed that 27 ClARF genes contain several stress response elements, suggesting that ClARFs might be involved in the abiotic stress response. Expression profile analysis shows that 27 ClARF genes were all expressed in the root, shoot, leaf, kernel, glume, and male flower of Coix with varying expression levels. Furthermore, qRT-PCR analyses revealed that the majority of ClARFs members were upregulated or downregulated in response to hormone treatment and abiotic stress. The current study expands our understanding of the functional roles of ClARFs in stress responses and provides basic information for the ClARF genes. [ABSTRACT FROM AUTHOR]

In order to screen out coix seed germplasm resources suitable for cultivation in Shanxi, 23 coix seed germplasm resources from different habitats in China were comprehensively analyzed for agronomic traits such as plant height, granulation layer, number of main stem nodes, branch node positions, and effective tiller number. Evaluation, in order to provide theoretical reference for the creation of new germplasm of Coix and the breeding of excellent new varieties. The experiment adopted a completely randomized design, and the agronomic trait data were comprehensively evaluated using the membership function method. The results showed that 23 coix seed germplasm resources had great differences in agronomic traits. After comprehensive evaluation using the membership function method, 8 coix seed germplasms with excellent comprehensive performance were screened out, which are suitable for use as excellent breeding materials and excellent parents of new varieties. [ABSTRACT FROM AUTHOR]

Teosinte is the closest wild ancestor of maize and is used as a valuable resource for taxonomical, evolutionary and genetic architectural studies of maize. Teosinte is also a repository of numerous diverse alleles for complex traits, including nutritional value and stress adaptation. Accessions including teosintes, maize inbred lines and coix were investigated for kernel protein and its association with DNA markers. The proposed investigation assumed that wild accessions had different genic/allelic content and consequently expression profile than modern maize because of the domestication syndrome and bottleneck effects. Total protein content in hard stony fruit case teosinte accessions were assessed from kernels with and without seed coats, while protein content from coix and maize lines was evaluated from kernels only. The accessions were also subjected to molecular profiling using 84 SSR markers, and obtained genotypic data were used for population structure and association analysis. The results emphasize that teosintes have higher protein content (18.5% to 26.29%), followed by coix (18.26%), and the least among maize lines (9% to 11%). Among teosintes, without-seed-coat samples had 3–6% higher protein content than with-seed-coat samples. When compared to other teosinte species, Z. mays subsp. mexicana accessions showed higher protein content, ranging from 18.62% to 26.29%. All evaluated accessions were divided into four subpopulations with K = 4, and seven significant (p < 0.01) marker–trait associations were seen with umc1294, umc1171, phi091, umc2182 and bnlg292 markers, which are distributed across chromosomes 4, 5, 7, 8 and 9, respectively. We have observed that the wild relatives carry protein content-enhancing alleles and can be used as productive donor parents in pre-breeding efforts to increase the protein content of maize. [ABSTRACT FROM AUTHOR]

Context: Coix [ Coix lacryma-jobi L. var. mayuen (Roman.) Stapf (Poaceae)], a crop of medicinal and edible significance, contains coixol, which has demonstrated anticancer properties. However, the limited solubility of coixol restricts its potential therapeutic applications., Objective: This study prepared a water-soluble coixol-β-cyclodextrin polymer (CDP) inclusion compound and evaluated its anticancer effect., Materials and Methods: The coixol-CDP compound was synthesized through a solvent-stirring and freeze-drying technique. Its coixol content was quantified using HPLC, and its stability was tested under various conditions. The anticancer effects of the coixol-CDP compound (4.129, 8.259, 16.518, and 33.035 mg/L for 24, 48, and 72 h) on the proliferation of non-small cell lung cancer (NSCLC) A549 cells were evaluated using an MTT assay; cell morphology was examined by Hoechst nuclear staining; apoptosis and cell cycle was detected by flow cytometry; and the expression of apoptosis-related proteins was assessed by Western blots., Results: The water-soluble coixol-CDP inclusion compound was successfully prepared with an inclusion ratio of 86.6% and an inclusion yield rate of 84.1%. The coixol content of the compound was 5.63% and the compound remained stable under various conditions. Compared to coixol alone, all 24, 48, and 72 h administrations with the coixol-CDP compound exhibited lower IC 50 values (33.93 ± 2.28, 16.80 ± 1.46, and 6.93 ± 0.83 mg/L) in A549 cells; the compound also showed stronger regulatory effects on apoptosis-related proteins., Discussion and Conclusions: These findings offer a new perspective for the potential clinical application of Coix in NSCLC therapy and its future research.

Teosinte is the closest wild ancestor of maize and is used as a valuable resource for taxonomical, evolutionary and genetic architectural studies of maize. Teosinte is also a repository of numerous diverse alleles for complex traits, including nutritional value and stress adaptation. Accessions including teosintes, maize inbred lines and coix were investigated for kernel protein and its association with DNA markers. The proposed investigation assumed that wild accessions had different genic/allelic content and consequently expression profile than modern maize because of the domestication syndrome and bottleneck effects. Total protein content in hard stony fruit case teosinte accessions were assessed from kernels with and without seed coats, while protein content from coix and maize lines was evaluated from kernels only. The accessions were also subjected to molecular profiling using 84 SSR markers, and obtained genotypic data were used for population structure and association analysis. The results emphasize that teosintes have higher protein content (18.5% to 26.29%), followed by coix (18.26%), and the least among maize lines (9% to 11%). Among teosintes, without-seed-coat samples had 3–6% higher protein content than with-seed-coat samples. When compared to other teosinte species, Z. mays subsp. mexicana accessions showed higher protein content, ranging from 18.62% to 26.29%. All evaluated accessions were divided into four subpopulations with K = 4, and seven significant (p < 0.01) marker–trait associations were seen with umc1294, umc1171, phi091, umc2182 and bnlg292 markers, which are distributed across chromosomes 4, 5, 7, 8 and 9, respectively. We have observed that the wild relatives carry protein content-enhancing alleles and can be used as productive donor parents in pre-breeding efforts to increase the protein content of maize.

Plant-based probiotic beverages have gained increasing interest due to demand from health-conscious consumers. In this study, we aimed to isolate and screen lactic acid bacteria possessing functional properties for use as a starter culture of fermented almond and coix beverages. Lactiplantibacillus plantarum L42g isolated from fermented beef was selected. Both intact cells and cell free supernatant of this strain exhibited high antioxidant activity based on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging at 38.2% and 44.9%, respectively. L. plantarum L42g grown in MRS broth supplemented with 1% (w v−1) monosodium glutamate (MSG) produced a large amount of γ-aminobutyric acid (GABA) at 496.7 μg mL−1. Moreover, strain L42g displayed remarkable antibacterial activity against several potential foodborne bacterial pathogens, including Bacillus cereus , Listeria monocytogenes , Listeria inocua , Staphylococcus aureus , Streptococcus agalactiae , Escherichia coli , Salmonella enterica subsp. enterica serovar Typhimurium, Shigella sp., Vibrio cholerae and Vibrio parahaemolyticus. Strain L42g also possessed additional probiotic properties including abilities to tolerate gastrointestinal conditions, adhere to gut mucosa, co-aggregate with pathogens, be susceptible to antibiotics, and produce protease. Probiotic strain L42g was subsequently employed in fermenting almond and coix juices containing MSG (1%) supplementation. Levels of antioxidant, GABA and antibacterial formation along with cell growth were clearly higher in fermented almond juice than in fermented coix juice. Nonetheless, both fermented almond and coix juices meet the standards required for the consumption of fermented beverages. Therefore, L. plantarum strain L42g represents a promising starter culture for producing functional plant-based probiotic beverages. [Display omitted] • Lactiplantibacillus plantarum strain L42g functions as a good starter culture. • L. plantarum L42g exhibits antioxidant, GABA-producing and antibacterial activities. • Almond and coix functional fermented drinks were produced using L. plantarum L42g. • First report of almond and coix beverages that have GABA supplement derived from lactic acid bacteria fermentation. [ABSTRACT FROM AUTHOR]

Coix lacryma-jobi var. ma-yuen L. Gramineae is widely cultivated in Taiwan. Literature regarding the molecular action mechanism of coixol on tyrosinase and the application of coicis seed extracts to the processing of facial masks is still lacking. Solvent extractability analysis revealed that most of the polyphenolics in coicis seeds were water soluble (3.17 ± 0.12 to 3.63 ± 0.07 μg/mLGAE). In contrast, the methanolic extract contained the most flavonoids (0.06 ± 0.00~0.26 ± 0.03 μg/mL QE) and coixol (11.43 ± 0.13~12.83 ± 0.14 μg/mL), showing potent antioxidant capability. Additionally, the contents of coixenolide (176.77 ± 5.91 to 238.60 ± 0.21 μg/g), phytosterol (52.45 ± 2.05 to 58.23 ± 1.14 mg/g), and polysaccharides (3.42 ± 0.10 to 4.41 ± 0.10 mg/g) were rather high. The aqueous extract (10 μg/mL) and the ethanolic extract (1 mg/mL) showed no cytotoxicity to B16F10 melanocytes. More attractively, the ethanolic extract at 1 mg/mL caused 48.4% inhibition of tyrosinase activity in B16F10 melanocytes, and 50.7% on human tyrosinase (hTyr) fragment 369–377. Conclusively, the coicis seed extracts containing abundant nutraceuticals with promising anti-hTyr activity and moisturizing capability can serve as good ingredients for facial mask processing. [ABSTRACT FROM AUTHOR]

Exposure to ionizing radiation (IR) can cause oxidative damage to human body, leading to various diseases and even death. In this study, the potential radioprotective effect of coix seed seedling extract (CSS‐E) was studied through a model of 60Co‐γ radiation‐induced oxidative stress in mice. Overall radioprotective effect of CSS‐E against radiation‐induced damage was evaluated by biochemical analysis and histopathological analysis. The results showed that CSS‐E could significantly reduce the IR‐induced damage to the hematopoietic system. CSS‐E‐M (200 mg/kg BW) pretreatment could increase the activities of superoxide dismutase in serum, liver, and spleen increased by 31.68%, 45.10%, and 56.67%, respectively, and the glutathione peroxidase levels in serum, liver, and spleen of mice were improved by 19.17%, 41.97%, and 130.56%, respectively. Meanwhile, the glutathione levels of serum, liver, and spleen in CSS‐E‐M group were increased by 17.10%, 35.06%, and 40.71%, respectively. The contents of MDA in different tissues and serum could be reduced by CSS‐E‐M treatment to the normal level. Moreover, CSS‐E could markedly reduce the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in radiation mice, among which CSS‐E‐M group showed maximum restoration with decreased AST and ALT levels by 20.13% and 32.76% as compared against IR group. In conclusion, these results indicated that CSS‐E could be used as a potential natural radioprotectant against IR‐induced damage. [ABSTRACT FROM AUTHOR]

Introduction: Treating older patients with acute appendicitis with multiple underlying diseases is a challenging situation in the clinical setting. Throughout the history of East Asian medicine, many texts have noted the use of herbal decoctions to successfully treat symptoms associated with appendicitis, thereby providing an alternative management strategy for treating frail patients with acute appendicitis., Case Presentation: This report presents the case of a 93-year-old male diagnosed with acute appendicitis with appendicolith who was unsuitable for surgical intervention due to poor lung condition and prolonged prothrombin time caused by Apixaban. By receiving complementary therapy consisting of traditional Chinese medicine (TCM) and antibiotics, he recovered successfully., Discussion: In the early stage of acute appendicitis, infection control and gastrointestinal mobility are the most important factors to consider. Scientific research has demonstrated that many herbs used in TCM formulas possess anti-inflammatory, anti-oxidative and immune attenuation effects. For many patients who are unsuitable for surgical intervention due to unfavorable conditions, such as the prolonged prothrombin time presented in this case, complementary treatment with TCM offers adjuvant therapeutical effects associated with infection control, and improvement of the gastrointestinal environment and function of elderly patients., (Copyright © 2024. Published by Elsevier Inc.)

Lipolysis is the hydrolysis of triglycerides (TGs), commonly known as fats. Intracellular lipolysis of TG is associated with adipose triglyceride lipase (ATGL), which provides fatty acids during times of metabolic need. The aim of this study was to determine whether Coix lacryma-jobi L. var. ma-yuen Stapf ( Coix ) sprouts (CS) can alleviate obesity through lipolysis. Overall, we investigated the potential of CS under in vitro and in vivo conditions and confirmed the underlying mechanisms. Huh7 cells were exposed to free fatty acids (FFAs), and C57BL/6J mice were fed a 60% high-fat diet. When FFA were introduced into Huh7 cells, the intracellular TG levels increased within the Huh7 cells. However, CS treatment significantly reduced intracellular TG levels. Furthermore, CS decreased the expression of Pparγ and Srebp1c mRNA and downregulated the mutant Pnpla3 (I148M) mRNA. Notably, CS significantly upregulated ATGL expression. CS treatment at a dose of 200 mg/kg/day resulted in a significant and dose-dependent decrease in body weight gain and epididymal adipose tissue weight. Specifically, the group treated with CS (200 mg/kg/day) exhibited a significant modulation of serum lipid biomarkers. In addition, CS ameliorated histological alterations in both the liver and adipose tissues. In summary, CS efficiently inhibited lipid accumulation through the activation of the lipolytic enzyme ATGL coupled with the suppression of enzymes involved in TG synthesis. Consequently, CS show promise as a potential anti-obesity agent.

Coix (Coix lacryma-jobi Linné. var. mayuen Stapf) is, unlike many herbs used in Chinese traditional medicines, often used as a single agent. Coix seed has its specific constituents coixenolide and coixol. This review summarizes the effects of coix seed on human pathology and physiology, and the roles of coixenolide and coixol on the effects. The comprehensive literature search was conducted using PubMed, Citation Information by National Institute of Informatics of Japan (CiNii), University Hospital Medical Information Network Clinical Trials Registry (UMIN-CTR), and ClinicalTrials.gov databases. Some studies suggest that coix seed promotes the spontaneous regression of viral infections of the skin. Meanwhile, kanglite, a purified oil agent used for cancer therapy, has been implied to increase the ratio of CD4+T cells in the peripheral blood of cancer patients undergoing treatment. These studies seem to indicate that coix seed may affect cellular immune function. However, there are limitations to the individual studies on which the above consideration is based, and thereby, it is not conclusive what specific immune or clinical indices coix seed affects. In addition, the effects of coixol on human immune function have not been clarified. Therefore, coix seed seems promising for health promotion and presents a challengeable and attractive theme for natural product chemists, pharmacologists, immunologists, and clinicians. [ABSTRACT FROM AUTHOR]

Coix lacryma-jobi var. ma-yuen L. Gramineae is widely cultivated in Taiwan. Literature regarding the molecular action mechanism of coixol on tyrosinase and the application of coicis seed extracts to the processing of facial masks is still lacking. Solvent extractability analysis revealed that most of the polyphenolics in coicis seeds were water soluble (3.17 ± 0.12 to 3.63 ± 0.07 μg/mLGAE). In contrast, the methanolic extract contained the most flavonoids (0.06 ± 0.00~0.26 ± 0.03 μg/mL QE) and coixol (11.43 ± 0.13~12.83 ± 0.14 μg/mL), showing potent antioxidant capability. Additionally, the contents of coixenolide (176.77 ± 5.91 to 238.60 ± 0.21 μg/g), phytosterol (52.45 ± 2.05 to 58.23 ± 1.14 mg/g), and polysaccharides (3.42 ± 0.10 to 4.41 ± 0.10 mg/g) were rather high. The aqueous extract (10 μg/mL) and the ethanolic extract (1 mg/mL) showed no cytotoxicity to B16F10 melanocytes. More attractively, the ethanolic extract at 1 mg/mL caused 48.4% inhibition of tyrosinase activity in B16F10 melanocytes, and 50.7% on human tyrosinase (hTyr) fragment 369–377. Conclusively, the coicis seed extracts containing abundant nutraceuticals with promising anti-hTyr activity and moisturizing capability can serve as good ingredients for facial mask processing.

Objective: To investigate the effective compounds, potential targets and molecular mechanism of Kanglaite injection (KLTi) in the treatment of Non-Small Cell Lung Cancer (NSCLC) based on network pharmacology. Methods: The active compounds and targets of KLTi which extracted and isolated from Coix Seed were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). The related genes of NSCLC were obtained by searching the Human Gene Database (GeneCards) and Online Mendelian Inheritance in Man (OMIM). The candidate targets of KLTi in the treatment of NSCLC were obtained after extracting the intersection network. The "drug-component-targetdisease" network was constructed with the help of Cytoscape 3.7.2. The Protein- Protein Interaction networks were constructed on the STRING platform and core network modules were screened. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of candidate genes were performed using Metascape platform, and a "pathway-target- compounds" network was constructed to further screen key genes and active compounds. Results: A total of 11 compounds, 22 candidate targets, 206 GO functions and 12 KEGG pathways were obtained. Conclusion: The active compounds of KLTi in the treatment of NSCLC are stigmasterol, stigmasterol a1 and ergosterol. The key targets are PGR, NCOA2, PTGS2, NR3C2, and PTGS1. The core GO functions included receptor activity and binding, neuronal signal transmission and hormone stimulation; KEGG mainly involves cancer pathways, neuroactive ligand-receptor interactions and calcium signaling pathways. This study reveals the molecular biological mechanism of KLTi in the treatment of NSCLC, which is speculated to be related to neuroendocrine, providing a new basis and therapeutic direction for subsequent clinical application and experimental research. [ABSTRACT FROM AUTHOR]

Abstract Background Coix lacryma-jobi var. ma-yuen (Rom.Caill.) Stapf has been used in China as an herbal medicine. Many studies of this plant have reported anti-proliferative and apoptotic activities on human cancer cell lines. Therefore, this study of the anti-metastatic effect of Coix lacryma-jobi var. ma-yuen Stapf sprout extract (CLSE) in colorectal cancer cells may provide a scientific basis for exploring anti-cancer effects of edible crops. Methods To evaluate the effect of CLSE on cell proliferation and signaling, we performed a Cell Counting Kit-8 (CCK-8) assay in HCT116 cells and used western blot analysis. Furthermore, scratch-wound healing, transwell migration, matrigel invasion, and adhesion assays were conducted to elucidate the anti-metastatic effects of CLSE under hypoxic conditions in colon cancer cells. Results First, CLSE decreased deferoxamine (DFO)-induced migration of colon cancer cells by 87%, and blocked colon cancer cell migration by 80% compared with hypoxia control cells. Second, CLSE treatment resulted in a 54% reduction in hypoxia-induced invasiveness of colon cancer cells, and 50% inhibition of adhesive potency through inactivation of the extracellular signal-regulated kinase (ERK) 1/2 and protein kinase b (AKT) pathways. Third, conditioned medium collected from CLSE-treated HCT116 cells suppressed tube formation of human umbilical vein endothelial cells (HUVECs) by 91%. Conclusions CLSE inhibited migration, invasion, and adhesion of colon cancer cells and tube formation by HUVECs via repression of the ERK1/2 and AKT pathways under hypoxic conditions. Therefore, CLSE may be used to treat patients with colon cancer.

Coix seed polysaccharides had received increasing attention due to their diverse biological activities. In this study, a homogeneous polysaccharide (CSPW) was extracted and purified from coix seed. Furthermore, the saliva-gastrointestinal digestion and fecal fermentation behavior of CSPW were simulated in vitro. The results showed that CSPW was mainly composed of glucose. It cannot be degraded by the simulated salivary and intestinal digestive system, but can be degraded by the simulated gastric digestive system. After fermentation for 24 h, CSPW promoted the production of short-chain fatty acids (SCFAs), with acetic acid, propionic acid and n-butyric acid being the main metabolites. In addition, CSPW could significantly regulate the composition and microbial diversity of gut microbiota by increasing the relative abundance of beneficial bacteria, such as Limosilicactobacillus, Bifidobacterium and Collinsella. Finally, further analysis of functional prediction revealed that amino acid metabolism, nucleotide metabolism and carbohydrate metabolism were the most important pathways for CSPW to promote health. In summary, our findings suggested that CSPW could potentially be used as a good source of prebiotics because it can be used by gut microbiota to produce SCFAs and regulate the gut microbiota., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)

As a common medicinal and edible resource in China, Coicis Semen has a long history of cultivation and medicinal use. Traditional Chinese medicine(TCM) clinically believes that Coicis Semen has the effect of strengthening the spleen and tonifying the lungs, clearing heat and dampness, removing pus and paralysis, and stopping diarrhea. Therefore, it is used to treat edema, foot odor, spleen deficiency, diarrhea, and other symptoms. The above effects are closely related to the active ingredients of Coicis Semen, such as esters, fatty acids, polysaccharides, proteins, as well as phenolic acids, sterols, flavonoids, lactams, triterpenes, alkaloids, and adenosine. Modern research has found that Coicis Semen also has anti-cancer, anti-inflammatory, antioxidant, hypoglycemic, and hypotensive effects and other pharmacological activities, and it can improve immunity and regulate lipid metabolism. Coicis Semen is widely distributed in China, mainly produced in Guizhou, Yunnan, Fujian, Sichuan, and other places, and the quality of Coicis Semen from different origins varies. From ancient times to the present, Coicis Semen processing methods have experienced the process from simple to complex, and the types of auxiliary materials are more extensive, such as soil, bran, and river sand. These processing methods have been inherited from generation to generation. Nowadays, the commonly used methods are bran-fried, stir-fried, sand-fried, etc. In this paper, by reviewing the relevant literature in China and abroad in recent years, the main active ingredients and related pharmacological effects of Coicis Semen are sorted out, and the effects of different origins and processing methods on the chemical composition of Coicis Semen are summarized, with a view to providing references for the comprehensive development and utilization of Coicis Semen and the further study of its mechanism of action.

Coix seed, a versatile agricultural product, is known for its nutritional and functional components. However, the common contamination with mycotoxins represents a potential risk for human health. A sensitive analytical method was developed and validated to simultaneously determine 26 mycotoxins, including regulated and emerging, using stable-isotope-dilution-assay and LC-MS/MS. The study found co-contamination in 100% of samples from Southeast China, with 8-15 different mycotoxins for each and a total of 20 for all. Probabilistic risk assessments indicated long-term health concerns, with Aflatoxin B 1 , ochratoxin A, and zearalenone being priority for risk control. Overall, this study appears to be the first to develop a rapid and robust analytical method of 26 mycotoxins and to conduct Monte Carlo simulation-based chronic risk assessments for 12 individual mycotoxins detected in coix seed, which would be of significance for risk communication as well as for regulatory authority in devising effective strategies to minimize exposure health risk., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Ethnopharmacological Relevance: Coix lacryma-jobi var. ma-yuen (Romanet du Caillaud) Stapf is a plant of the genus Coix in the Gramineae family. Coix seed is cultivated in various regions throughout China. In recent years, with the research on the medicinal value of Coix seed, it has received more and more widespread attention from people. Numerous pharmacological effects of Coix seed have been demonstrated through modern pharmacological studies, such as hypoglycemia, improving liver function, anti-tumor, regulating intestinal microbiota, improving spleen function, and anti-inflammatory effects., Aims of the Study: This article is a literature review. In recent years, despite the extensive research on Coix seed, there has yet to be a comprehensive review of its traditional usage, medicinal resources, chemical components, and pharmacological effects is still lacking. To fill this gap, the paper provides an overview of the latest research progress on Coix seed, aiming to offer guidance and references for its further development and comprehensive utilization., Material and Methods: To gather information on the traditional usage, phytochemical ingredients, and pharmacological properties of Coix seed, we conducted a literature search using both Chinese and English languages in five databases: PubMed, Web of Science, China National Knowledge Infrastructure (CNKI), and Springer., Results: This article is a literature review. The chemical constituents of Coix seed include various fatty acids, esters, polysaccharides, sterols, alkaloids, triterpenes, tocopherols, lactams, lignans, phenols, flavonoids and other constituents. Modern pharmacological research has indeed shown that Coix seed has many pharmacological effects and is a natural anti-tumor drug. In addition to its anti-tumor effect, it also has pharmacological effects such as hypoglycemia, improving liver function, regulating intestinal microbiota, improving spleen function, and anti-inflammatory effects., Conclusions: This article provides a brief overview of the traditional uses, biotechnological applications, chemical components, and pharmacological effects of Coix seed. It highlights the importance of establishing quality standards, discovering new active ingredients, and exploring pharmacological mechanisms in Coix seed research. The article also emphasizes the significance of clinical trials, toxicology studies, pharmacokinetics data, and multidisciplinary collaboration for further advancements in this field. Overall, it aims to enhance understanding of Coix seed and its potential in pharmaceutical development and wellness products., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

The mechanisms underlying the distribution of many toxic metal(loid)s in shoots and metal(loid) transport to grains have been well documented in the quest for food safety but there remains a lack of knowledge on thallium (Tl) accumulation in food crops. Here, field investigations combined with a glasshouse pot experiment were conducted to investigate the characteristics of Tl distribution and accumulation in coix, a major food crop in south Guizhou province, China, and the role of node I in restricting Tl transport to the seed. Fourteen percent of coix seed samples collected from the Lanmuchang Tl-As-Hg mine contained higher Tl concentrations than the recommended limit for foods and feedstuffs in Germany (0.5 mg kg -1 ), with the highest exceedance rate of the metal(loid)s determined, when grown in soils surrounding the mine with a very high Tl concentration of 0.07-89.5 mg kg -1 and a general low pH of 4.19-6.48. Thallium concentrations were higher in coix nodes than in internodes, followed by roots and grains. The Tl translocation factors from node I to grains were 0.01-0.21 and were the lowest of any translocation factors between different tissues. Node I is therefore the key tissue restricting Tl transport to coix grains. Thallium was localized mainly in the diffuse vascular bundles (DVBs) in node I. The co-localization of Tl and sulfur in the DVBs and Tl contamination-induced phytochelatin (PC) accumulation indicate that Tl storage in the DVBs involving complexation with PCs in node I is an important process in Tl accumulation in coix grains. Moreover, the area of DVBs in node I increased with increasing soil Tl pollution level, providing more channels for Tl transport to the panicles and grains and thereby acting as a key factor restricting Tl transport to the grains. These results provide new insights into the key role of node I in Tl accumulation in coix grains and indicate key points to minimize Tl accumulation in grains for food safety., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Background: Pain is one of the most commonly reported symptoms in patients with advanced cancer, but is still less than optimally treated. The effect of traditional Chinese medicine in cancer pain treatment is nowadays getting more and more attention.Objective: To investigate the effect of a coix seed oil injection on cancer pain relief in a cancer center in a tertiary hospital in China.Methods: Patients in the treatment group received a coix seed oil injection for 2 weeks, while patients in the control group received equivalent 0.9% saline. The numeric rating scale was used to assess the pain level. The Quality of Life Questionnaire-Core 30 was used to assess life quality. The adverse drug reactions during the treatment process were observed.Results: Patients in the coix seed treatment group had significantly superior efficacy on pain control over those in the control group. Coix seed therapy significantly improved patients' scores reflecting by the Quality of Life Questionnaire-Core 30 (QLQ-C30) scale. In addition, the occurrence of adverse reactions such as constipation and nausea in the treatment group was significantly lower than that in the control group.Conclusion: The coix seed oil injection effectively reduced the pain level of cancer patients, significantly improved their life quality, and had no obvious adverse effects. [ABSTRACT FROM AUTHOR]

Highlights • Main nutrient content of WC is higher than that of CC. • The proteome and transcriptome of coix during filling stage were analyzed. • A total of 20,039 DEGs and 1,444 DEPs were isolated in WC and CC. • Several metabolism pathways were found to explain the difference of coix seed. Abstract In present study, the content of main nutrients in wild coix seed were significantly higher than cultivated coix seed. Transcriptome and proteome were combined to provide new insight of the molecular mechanisms linked to nutritional quality of wild coix seed and cultivated coix seed by RNA sequencing and isobaric tags for relative and absolute quantification techniques. A total of 20,039 genes and 471 proteins exhibited differential expression level in cultivated coix seed when compared with wild coix seed. These genes and proteins revealed that the pathway of flavonoids biosynthesis, starch and sucrose metabolism, lipid metabolism and amino acid metabolism were linked to nutritional quality of coix seed. Our results provided transcriptomics and proteomics information with respect to the molecular mechanisms of nutritional changes of coix seed, identified key genes and proteins that associated with the metabolism and accumulation of nutrients, and helped clarify the mechanisms of nutrient differences. [ABSTRACT FROM AUTHOR]

Coixol, a plant polyphenol extracted from coix (Coix lachryma-jobi L.var.ma-yuen Stapf), has not been investigated for its anti-inflammatory effect. In this study, using a lipopolysaccharide (LPS)-induced macrophage cell model, we observed that coixol can effectively reduce the expression of interleukin (IL)-1β, IL-6, IL-18, tumor necrosis factor (TNF)-α, nitric oxide (NO), inducible nitric oxide synthases (iNOS), and cyclooxygenase (COX)-2, but had no effect on the expression of the anti-inflammatory mediator IL-10. Furthermore, we found that coixol inhibits mitogen-activated protein kinases (MAPKs), nuclear transcription factor κ B (NF-κB) pathways, and NOD-like receptor protein (NLRP) 3 inflammasome activation. In conclusion, the present study demonstrates that coixol exerts certain anti-inflammatory effects by inhibiting the expression of pro-inflammatory mediators in vitro. The mechanism of this effect was in part related to its ability to inhibit the activation of NF-κB, MAPKs pathways, and NLRP3 inflammasome.

Adlay (Coix lachryma-jobi L.) is a traditional Chinese herbal medicine with various biological activities. We investigated the anti-diabetic effects of different parts of adlay seeds, including polished adlay (PA), adlay bran (AB) and dehulled adlay (DA) in a streptozotocin (STZ)/high fat diet (HFD) diabetic rat model (DM). DM rats supplemented with or without PA (43%), AB (3%), or DA (46%) diet for 8 weeks. The plasma glucose and insulin levels and the insulin resistance index (HOMA-IR) were increased in DM group; among the three adlay diets, DA has the best effects attenuating all of these alterations in DM rats. Both AB and DA alleviated diabetes-impaired glucose tolerance. The increased hepatic phosphoenolpyruvate carboxykinase protein expression in DM group was improved by all of the three adlay diets. The increased ratio of glucose-6-phosphatase to glucokinase in DM group was suppressed by DA supplementation, further suggesting DA diet is most effective among the three diets. Both AB and DA diets had beneficial effects against hepatic steatosis, with better effects observed in DA group. These results suggest that the DA diet, composed of both polished adlay and adlay bran, possesses the best potential to improve glucose homeostasis, at least in part, by alleviating hepatic glucose metabolism and steatosis.

The enhanced therapeutic effects and mechanisms of certain herbal combination in various herbal prescriptions are mostly unclear. A combination of two herbs, namely Ephedrae herba (EH) and Coicis semen (CS), has been commonly prescribed for obesity. In our previous work, the combination of EH and CS was studied using network pharmacological approach to predict its pharmacological targets and in vitro experiments to evaluate its efficacy on obesity. Although we demonstrated enhanced anti-adiposity effects of the combination on matured adipocytes, the molecular mechanisms and contributing compounds underlying the effects of EH-CS combination on adiposity or adipogenesis were not fully elucidated. The current study adopted integrated bioinformatics analysis to precisely validate potential targets of EH-CS by screening differentially expressed genes (DEGs) of morbid obesity patients from NCBI gene expression omnibus (GEO). Based on the functional cluster analysis of down-regulated DEGs, the anti-adipogenesis mechanism of EH-CS combination was speculated with KEGG enrichment analysis. Furthermore, we investigated the combinational effects of EH and coixol, or stigmasterol, the two compounds in CS which were expected to have main beneficial effects in metabolic diseases. Moreover, distinct effect of the combination on transcriptional activity of glucocorticoid receptor (GR) was investigated using electrophoretic mobility shift assay (EMSA). The EH-CS combination was predicted to modulate down-regulated genes which are involved in KEGG pathways crucial to metabolic disease in morbidly obese individuals. The combination of EH with CS compounds significantly increased the phosphorylation of acetyl-coA carboxylase (ACC), AMP-activated protein kinase (AMPK), and protein kinase B (AKT) in 3T3-L1 cells and decreased intracellular lipid accumulation. The two CS compounds significantly increased the anti-adipogenesis/lipogenesis effects of EH by inhibiting the gene expression levels. Finally, the combination of EH and coixol inhibited dexamethasone-induced GR translocation to the nucleus and transcriptional binding activity in adipocytes. The combination of EH and CS could be considered a therapeutic strategy for treating metabolic diseases, including obesity., (© 2023. The Author(s).)

Although a series of physio-biochemical changes of coix seed occur during the storage, the underlying mechanism remains unknown. The present study aimed to investigate the aging mechanism of coix seed during storage. Proteome patterns of coix seed stored for 1-month, 5-month and 10-month at room temperature were compared using 2-dimensional gel electrophoresis (2-DE) and mass spectra. Thirty-one differentially expressed proteins (DEPs) were detected, which involved seven pathways including starch and sucrose metabolism, carbon metabolism, RNA transport, proteasome, protein processing in endoplasmic reticulum, ribosome, and RNA degradation. Sucrose synthase 1 was associated with sucrose metabolism and affected the sucrose content during the storage. Increased ambient temperature enhanced respiration of coix after 5-month storage, and overexpression of aconitate hydratase 2 promoted the generation of energy. In addition, the proteins involved in the antioxidant system and resistance stimulus of coix seed were mostly up-regulated during storage. [ABSTRACT FROM AUTHOR]

Overconsumption of traditional fats and oils may cause many chronic diseases such as obesity and cardiovascular diseases due to their unhealthy or imbalanced fatty acids. In the present study, a new functional trans-free lipid with low calories was synthesized through coix seed oil (CO), fully hydrogenated palm oil (FHPO) and Cinnamomum camphora seed oil (CCSO) in mass ratios of 45:45:10, 50:40:10 and 55:35:10 which catalyzed by Lipozyme RM IM. After reaction, the structured lipids were characterized by fatty acid composition, triacylglycerol (TAG) composition, melting and crystallization profiles, solid fat content (SFC), microstructure and polymorphic form. Results revealed that the total fatty acids of interesterified fats consisted of palmitic acid (28.74–32.02%) and oleic acid (21.72–29.80%), stearic acid (14.17–16.47%), linoleic acid (12.92–18.13%), and medium-chain fatty acids (MCFAs, 6.24–10.84%). As for TAG composition in interesterified fats, TAG species showed desirable amounts of OOL/OLO (17.36–20.29%), LLO/LOL/LaPO/LaOP/OLaP (8.22–10.81%), PLO/OPL/POL/PPL/PLP (14.84–15.98%) and POS/PSO/OPS/PPS/PSP (12.81–22.81%), where the content of POS/PSO/OPS/PPS/PSP, OOL/OLO and LLO/LOL/LaPO/LaOP/OLaP were much higher than that of physical blends. Besides, new TAGs species (PLO/OPL/POL/PPL/PLP) were observed after interesterification. Meanwhile, experimental studies also demonstrate that the crystal morphology of interesterified fats was small and discrete compared to those of the physical blends and its SMP (31.50–42.75 °C) decreased. Moreover, it should mention that SFC of the interesterified fat, ranging from 22.98% to 34.62% at 25 °C with most β’ crystal forms, was beneficial to improve the spreadability in term of shortening. Such a plastic fat synthesized by CO, FHPO and CCSO might be beneficial for food product. [ABSTRACT FROM AUTHOR]

Summary: In recent years, adlay water extract and related products have been widely consumed. This study investigated the chemical components and bioactive properties of adlay water extract at different germination stages. Glucose and maltose were significantly increased, whereas fructose was slightly decreased after germination. The contents of vitamins C and B1 in the water extract increased 1.61‐ and 2.68‐fold, respectively. The amounts of peptides, especially those with a molecular weight under 3000 Da, were significantly increased after germination. During germination, the level of total free amino acids rose from 54.09 to 128.22 μg mL−1, and the percentage of essential amino acids was elevated from 21.63% to 42.13%. Germination also substantially augmented the total phenolic content and antioxidant activity of the water extract. Moreover, the germinated adlay water extract showed higher inhibitory activity on angiotensin‐converting enzyme (ACE), xanthine oxidase and tyrosinase than the water extract of nongerminated adlay. These findings revealed that germination might be a potential way to improve the nutritional value and bioactive properties of adlay water extract. [ABSTRACT FROM AUTHOR]

The structure characteristics of three Coix seed (BCS, SCS, and TCS) prolamins were studied and their pure films properties compared. The three prolamins showed different amino acid composition, and hydrophobicity, and the results of LC-MS/MS exhibited their peptide sequence differences. Their aggregates were rodlike structures, formed very a fine meshwork and had self-assembly properties, which was the basis of their film-forming, similar to zeins. The SCS and TCS prolamins showed better thermal stability than that of BCS prolamins, and their films had better water resistance, puncture, and tensile strengths than those of pure zein film. The SCS film displayed better water absorption and higher elongation than those of the TCS film. Although some properties of the films, such as flexibility and thermal stability, still need to be improved, these results have proven that Coix seed prolamins have potential as biodegradable films for use in the food industry. [ABSTRACT FROM AUTHOR]

This paper presents an optimal method and verification process for measuring contents of 15 metal elements (boron, magnesium, aluminum, calcium, chromium, manganese, iron, cobalt, nickel, copper, zinc, cadmium, tin, antimony, and lead) in two kinds of coix seeds with microwave digestion-inductively coupled plasma-mass spectrometry. The results showed that microwave digestion-inductively coupled plasma-mass spectrometry was a simple and convenient method, which can accurately determine contents of 15 metal elements in coix seed, with detection limit ranging within 0.0016-7.02 mg/L, and recovery rate varying within 96.00-104.87%. National primary standard substance-green onions (GBW10049 GSB-27) were adopted to verify accuracy and precision of such method. The two tested coix seeds (Xingren smaller coix seed and Yunnan bigger coix seed) both contain Mn, Zn, Ca, Mg, Fe in high content, wherein the Xingren smaller coix seed is richer in Al, Cr, Ni, Cu, and Mg as compared to Yunnan bigger coix seed. Contents of heavy metals such as Ni, Cr, Pb, Cd, Sn, and Sb are in line with GB 2762-2012 of 'limits of contaminants in foods.' This method which can simultaneously determining 15 essential and nonessential elements in coix seed enjoys an acceptable analytical performance and is applicable for routine laboratory testing. Practical applications This paper studies a new method for simultaneous analysis and detection of 15 metal elements in coix seed with microwave digestion-inductively coupled plasma-mass spectrometry. The method features rapid analysis speed and wide linear range. After one-time sample digestion, 15 elements in coix seed can be determined simultaneously with high accuracy and sensitivity. [ABSTRACT FROM AUTHOR]

Background Coix ( Coix lachryma-jobi L.), commonly known as adlay or Job's tears, is a minor cereal and an important food item in some parts of Asia. It has also been used in the traditional Chinese medicine for relieving various ailments. Lack of systematic information on the composition and health effects greatly hinders the development of coix as a sustainable crop. Scope and approach This review summarises the chemical composition and health effects of coix. Various parts of coix seeds including bran, hull, and testa are studied. Research on the health effects of coix includes both in vitro and in vivo studies. The relationships between the bioactivities from the in vitro and the in vivo studies are discussed. The claimed health effects and chemical constituents of coix are compared with other common cereals. Key findings and conclusions Coix seeds contain a range of bioactive components including polysaccharides, proteins, lipids, polyphenols, phytosterols, carotenoids, spiroenone and lactams. The chemical constituents of coix contribute to a range of medical and nutritional benefits. These claimed health benefits include anti-oxidation, anti-cancer, anti-inflammation, anti-allergy, enhancing immunological activity, regulating endocrine functions, anti-obesity, anti-diabetes, gastroprotection, hypolipidemia, and modulating gut microbiota. The bioactivity of coix depends on the varieties as well as the sample preparation methods such as the solvent type. Coix seed is rich in starch and has similar processing properties of other cereals for food formulation. Coix can be further developed as a healthy grain for human consumption. [ABSTRACT FROM AUTHOR]