25 results on '"Kajarabille,N"'
Search Results
2. List of Contributors
- Author
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Alvarez-Suarez, P., primary, Anand, S., additional, Aral, A.L., additional, Begega, A., additional, Beurskens, R., additional, Bonavita, S., additional, Cohen, R.W., additional, Cowan, M., additional, Cruz, A., additional, Cuesta, M., additional, Dalecki, M., additional, Damasceno, W.C., additional, Díaz-Castro, J., additional, Fannon-Pavlich, M.J., additional, Fonseca, C.G., additional, Hasegawa, H., additional, Hernandez, R.A., additional, Herting, M.M., additional, Hijano, S., additional, Hiura, M., additional, Ichiyama, R.M., additional, Kajarabille, N., additional, Keenan, M.F., additional, Kunstetter, A.C., additional, Leelayuwat, N., additional, Mandyam, C.D., additional, Martins, R.N., additional, Moreno-Fernández, J., additional, Nariai, T., additional, Ochoa, J.J., additional, Pedroso, R.V., additional, Perrey, S., additional, Pinar, L., additional, Prieto, G.A., additional, Pulido-Morán, M., additional, Ravikiran, T., additional, Sampedro-Piquero, P., additional, Sehm, B., additional, Shah, T.M., additional, Shanmugam, M.S., additional, Snigdha, S., additional, Somkuwar, S.S., additional, Stein, A.M., additional, Tedeschi, G., additional, Tierney, W.M., additional, Uhlendorf, T.L., additional, Vani, R., additional, Wanner, S.P., additional, Watson, J., additional, Welman, K.E., additional, Yoder, A., additional, and Zheng, X., additional
- Published
- 2017
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3. Cytokine distribution in mothers and breastfed children after omega-3 LCPUFAs supplementation during the last trimester of pregnancy and the lactation period: A randomized, controlled trial
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Rodriguez-Santana, Y., primary, Ochoa, J.J., additional, Lara-Villoslada, F., additional, Kajarabille, N., additional, Saavedra-Santana, P., additional, Hurtado, J.A., additional, Peña, M., additional, Diaz-Castro, J., additional, Sebastian-Garcia, I., additional, Machin-Martin, E., additional, Villanueva, M., additional, Ramirez-Garcia, O., additional, and Peña-Quintana, L., additional
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- 2017
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4. PS-053 Variability In Adipokines Profile Of Newborns And Their Mothers After Dha Supplementation In Pregnancy
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Martín Alvarez, E, primary, Peña-Caballero, M, additional, Hurtado-Suazo, JA, additional, Kajarabille, N, additional, Lara-Villoslada, F, additional, and Ochoa, JJ, additional
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- 2014
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5. Influence of iron deficiency anaemia on bone metabolism
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Díaz-Castro, J., Silvia Hijano, Pulido-Moran, M., Kajarabille, N., and Ochoa, J. J.
6. Effect of oropharyngeal colostrum administration on immunoglobulin a levels in preterm newborns,Efectos de la administración de calostro orofaríngeo en recién nacidos prematuros sobre los niveles de inmunoglobulina A
- Author
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Martin Álvarez, E., Jiménez Cabanillas, M. V., Peña Caballero, M., Serrano López, L., Kajarabille, N., Javier Díaz-Castro, Ochoa Herrera, J. J., and Maldonado Lozano, J.
7. A New Approach to Oxidative Stress and Inflammatory Signaling during Labour in Healthy Mothers and Neonates
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Mario Pulido-Moran, Olga Ocón, Naroa Kajarabille, Sonia Prados, Jesús Florido, Julio J. Ochoa, Catalina de Paco, Javier Díaz-Castro, [Díaz-Castro,J, Kajarabille,N, Pulido-Moran,M, Ochoa,JJ] Department of Physiology, University of Granada, Granada, Spain. Institute of Nutrition and Food Technology 'Jose Mataix', University of Granada, Granada, Spain. [Florido,J, Prados,S, de Paco,C, and Ocon,O] Department Obtetrics and Gynecology, School of Medicine, University of Granada, Granada, Spain. Service of Obstetrics and Gynecology, University Hospital San Cecilio, Granada, Spain.
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Trabajo de parto ,Aging ,Glutatión peroxidasa ,Physiology ,Edad gestacional ,medicine.disease_cause ,Biochemistry ,Umbilical cord ,Umbilical vein ,Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings] ,Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Tumor Necrosis Factors::Tumor Necrosis Factor-alpha [Medical Subject Headings] ,Named Groups::Persons::Parents::Mothers [Medical Subject Headings] ,Pregnancy ,Phenomena and Processes::Metabolic Phenomena::Metabolism::Oxidative Stress [Medical Subject Headings] ,Catalasa ,Labor, Obstetric ,lcsh:Cytology ,Femenino ,Estrés oxidativo ,General Medicine ,Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Superoxide Dismutase [Medical Subject Headings] ,Catalase ,Fetal Blood ,Sangre fetal ,Superóxido dismutasa ,Humanos ,Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Glutathione Peroxidase [Medical Subject Headings] ,medicine.anatomical_structure ,Gestation ,Female ,Artery ,Signal Transduction ,Research Article ,Adult ,Article Subject ,Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Reproductive Physiological Processes::Reproduction::Pregnancy::Labor, Obstetric [Medical Subject Headings] ,Mothers ,Gestational Age ,Dinoprostona ,Named Groups::Persons::Age Groups::Infant::Infant, Newborn [Medical Subject Headings] ,Chemicals and Drugs::Biological Factors::Intercellular Signaling Peptides and Proteins::Cytokines::Interleukins::Interleukin-6 [Medical Subject Headings] ,Chemicals and Drugs::Biological Factors::Inflammation Mediators::Autacoids::Eicosanoids::Prostaglandins::Prostaglandins E::Dinoprostone [Medical Subject Headings] ,Dinoprostone ,Interleucina-6 ,Phenomena and Processes::Reproductive and Urinary Physiological Phenomena::Reproductive Physiological Phenomena::Gestational Age [Medical Subject Headings] ,Proinflammatory cytokine ,Anatomy::Fluids and Secretions::Body Fluids::Blood::Fetal Blood [Medical Subject Headings] ,Recién nacido ,medicine ,Humans ,lcsh:QH573-671 ,Fetus ,Glutathione Peroxidase ,business.industry ,Interleukin-6 ,Superoxide Dismutase ,Tumor Necrosis Factor-alpha ,Phenomena and Processes::Cell Physiological Phenomena::Cell Physiological Processes::Signal Transduction [Medical Subject Headings] ,Infant, Newborn ,Cell Biology ,medicine.disease ,Factor de necrosis tumoral alfa ,Oxidative Stress ,Check Tags::Female [Medical Subject Headings] ,Transducción de señal ,Immunology ,Chemicals and Drugs::Enzymes and Coenzymes::Enzymes::Oxidoreductases::Peroxidases::Catalase [Medical Subject Headings] ,Madres ,business ,Oxidative stress - Abstract
The objective of the current study was to investigate for the first time and simultaneously the oxidative stress and inflammatory signaling induced during the delivery in healthy mothers and their neonates. 56 mothers with normal gestational course and spontaneous delivery were selected. Blood samples were taken from mother (before and after delivery) both from vein and artery of umbilical cord. Lower antioxidant enzymes activities were observed in neonates compared with their mothers and lower oxidative stress in umbilical cord artery with respect to vein. There was an overexpression of inflammatory cytokines in the mother, such as IL-6 and TNF-α, and, in addition, PGE2was also increased. Neonates showed lower levels of IL-6 and TNF-αand higher values of sTNF-RII and PGE2in comparison with their mothers. Parturition increases oxidative damage in the mother, although the indicators of oxidative damage were lower in umbilical cord artery with respect to umbilical vein. The overexpression of inflammatory cytokines reveals that fetus suffers its own inflammatory process during parturition.
- Published
- 2015
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8. Content and Availability of Minerals in Plant-Based Burgers Compared with a Meat Burger.
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Latunde-Dada GO, Kajarabille N, Rose S, Arafsha SM, Kose T, Aslam MF, Hall WL, and Sharp PA
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- Humans, Animals, Cattle, Caco-2 Cells, Iron analysis, Zinc, Plants, Minerals, Meat Products analysis
- Abstract
Increasing numbers of individuals follow plant-based diets. This has sparked interest in the nutritional evaluation of the meat substitute sector. Nutritional understanding of these products is vital as plant-based eating becomes more common. For example, animal products are rich sources of iron and zinc, and plant-based foods could be inadequate in these minerals. The main aim was to analyse the mineral composition and absorption from a range of plant-based meat-free burgers and compare them to a typical beef burger. Total and bioaccessible mineral contents of plant-based burgers and a beef burger were determined using microwave digestion and in vitro simulated gastrointestinal digestion, respectively. Mineral bioavailability was analysed by in vitro simulated gastrointestinal digestion of foods, followed by exposure of Caco-2 cells to the sample digests and assessment of mineral uptake. Mineral quantification for all samples was achieved using inductively coupled ICP-optical emission spectrometry (ICP-OES). The content of minerals varied significantly amongst the burgers. Significantly greater quantities of Fe and Zn were found in the beef burger compared to most meat substitutes. Bioaccessible Fe was significantly higher in the beef compared to most of the plant-based meat alternatives; however, bioavailable Fe of most plant-based burgers was comparable to beef ( p > 0.05). Similarly, bioaccessible Zn was significantly ( p < 0.001) higher from the beef burger. Moreover, beef was superior regarding bioavailable Zn ( p ≤ 0.05-0.0001), with only the mycoprotein burger displaying comparable Zn bioavailability ( p > 0.05). Beef is an excellent source of bioaccessible Fe and Zn compared to most plant-based substitutes; however, these plant-based substitutes were superior sources of Ca, Cu, Mg and Mn. The quantity of bioaccessible and absorbable Fe varies dramatically among the meat alternatives. Plant-based burgers have the potential to provide adequate quantities of iron and zinc to those consuming such burgers as part of a varied diet. Thus, guiding consumer choices will depend on the variety of the vegetable constituents and their iron nutritional quality in different burgers.
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- 2023
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9. Ubiquinol Short-Term Supplementation Prior to Strenuous Exercise Improves Physical Performance and Diminishes Muscle Damage.
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Moreno-Fernandez J, Puche-Juarez M, Toledano JM, Chirosa I, Chirosa LJ, Pulido-Moran M, Kajarabille N, Guisado IM, Guisado R, Diaz-Castro J, and Ochoa JJ
- Abstract
The benefits of physical exercise on health are diminished when it is non-planned, strenuous, or vigorous, which causes an increase in oxygen consumption and production of free radicals, particularly serious at the muscular level. Ubiquinol could help achieve an antioxidant, anti-inflammatory, and ergogenic effect. The aim of this study is to evaluate whether a supplementation of ubiquinol during a short period could have a positive effect on muscle aggression, physical performance, and fatigue perception in non-elite athletes after high intensity circuit weight training. One hundred healthy and well-trained men, (firemen of the Fire Department of Granada) were enrolled in a placebo-controlled, double-blinded, and randomized study, and separated into two groups: the placebo group (PG, n = 50); and the ubiquinol group (UG, n = 50), supplemented with an oral dose. Before and after the intervention, data related to the number of repetitions, muscle strength, and perceived exertion, as well as blood samples were collected. An increase was observed in the UG regarding average load and repetitions, revealing an improvement in muscle performance. Ubiquinol supplementation also reduced muscle damage markers, showing a protective effect on muscle fibers. Therefore, this study provides evidence that ubiquinol supplementation improves muscle performance and prevents muscle damage after strenuous exercise in a population of well-trained individuals who are not elite athletes.
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- 2023
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10. Key Aspects in Nutritional Management of COVID-19 Patients.
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Fernández-Quintela A, Milton-Laskibar I, Trepiana J, Gómez-Zorita S, Kajarabille N, Léniz A, González M, and Portillo MP
- Abstract
This review deals with the relationship among nutrition, the immune system, and coronavirus disease 2019 (COVID-19). The influence of nutrients and bioactive molecules present in foodstuffs on immune system activity, the influence of COVID-19 on the nutritional status of the patients, and the dietary recommendations for hospitalized patients are addressed. Deficient nutritional status is probably due to anorexia, nausea, vomiting, diarrhea, hypoalbuminemia, hypermetabolism, and excessive nitrogen loss. There is limited knowledge regarding the nutritional support during hospital stay of COVID-19 patients. However, nutritional therapy appears as first-line treatment and should be implemented into standard practice. Optimal intake of all nutrients, mainly those playing crucial roles in immune system, should be assured through a diverse and well-balanced diet. Nevertheless, in order to reduce the risk and consequences of infections, the intakes for some micronutrients may exceed the recommended dietary allowances since infections and other stressors can reduce micronutrient status. In the case of critically ill patients, recently published guidelines are available for their nutritional management. Further, several natural bioactive compounds interact with the angiotensin-converting enzyme 2 (ACE2) receptor, the gateway for severe acute respiratory syndrome (SARS) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Natural bioactive compounds can also reduce the inflammatory response induced by SARS-CoV-2. These compounds are potential beneficial tools in the nutritional management of COVID-19 patients.
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- 2020
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11. Programmed Cell-Death by Ferroptosis: Antioxidants as Mitigators.
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Kajarabille N and Latunde-Dada GO
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- Animals, Antioxidants pharmacology, Biomarkers, Fatty Acids metabolism, Humans, Hydrogen Peroxide metabolism, Iron metabolism, Lipid Peroxidation drug effects, NF-E2-Related Factor 2 metabolism, Oxidation-Reduction, Oxidative Stress drug effects, Polyphenols, Reactive Oxygen Species metabolism, Vitamin E metabolism, Apoptosis drug effects, Ferroptosis drug effects
- Abstract
Iron, the fourth most abundant element in the Earth's crust, is vital in living organisms because of its diverse ligand-binding and electron-transfer properties. This ability of iron in the redox cycle as a ferrous ion enables it to react with H
2 O2 , in the Fenton reaction, to produce a hydroxyl radical (•OH)-one of the reactive oxygen species (ROS) that cause deleterious oxidative damage to DNA, proteins, and membrane lipids. Ferroptosis is a non-apoptotic regulated cell death that is dependent on iron and reactive oxygen species (ROS) and is characterized by lipid peroxidation. It is triggered when the endogenous antioxidant status of the cell is compromised, leading to lipid ROS accumulation that is toxic and damaging to the membrane structure. Consequently, oxidative stress and the antioxidant levels of the cells are important modulators of lipid peroxidation that induce this novel form of cell death. Remedies capable of averting iron-dependent lipid peroxidation, therefore, are lipophilic antioxidants, including vitamin E, ferrostatin-1 (Fer-1), liproxstatin-1 (Lip-1) and possibly potent bioactive polyphenols. Moreover, most of the enzymes and proteins that cascade or interact in the pathway of ferroptosis such as a subunit of the cystine/glutamate transporter xc - (SLC7A11), glutathione peroxidase 4 (GPX4), and the glutamate-cysteine ligase (GCLC) iron metabolism genes transferrin receptor 1 (TfR1) ferroportin, (Fpn) heme oxygenase 1 (HO-1) and ferritin are regulated by the antioxidant response element of the transcription factor, Nrf2. These, as well as other radical trapping antioxidants (RTAs), are discussed in the current review., Competing Interests: The authors declare no conflict of interest.- Published
- 2019
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12. Relationship between Changes in Microbiota and Liver Steatosis Induced by High-Fat Feeding-A Review of Rodent Models.
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Gómez-Zorita S, Aguirre L, Milton-Laskibar I, Fernández-Quintela A, Trepiana J, Kajarabille N, Mosqueda-Solís A, González M, and Portillo MP
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- Animals, Disease Models, Animal, Liver metabolism, Liver microbiology, Non-alcoholic Fatty Liver Disease microbiology, Rodentia, Diet, High-Fat adverse effects, Gastrointestinal Microbiome, Non-alcoholic Fatty Liver Disease etiology
- Abstract
Several studies have observed that gut microbiota can play a critical role in nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH) development. The gut microbiota is influenced by different environmental factors, which include diet. The aim of the present review is to summarize the information provided in the literature concerning the impact of changes in gut microbiota on the effects which dietary fat has on liver steatosis in rodent models. Most studies in which high-fat feeding has induced steatosis have reported reduced microbiota diversity, regardless of the percentage of energy provided by fat. At the phylum level, an increase in Firmicutes and a reduction in Bacteroidetes is commonly found, although widely diverging results have been described at class, order, family, and genus levels, likely due to differences in experimental design. Unfortunately, this fact makes it difficult to reach clear conclusions concerning the specific microbiota patterns associated with this feeding pattern. With regard to the relationship between high-fat feeding-induced changes in liver and microbiota composition, although several mechanisms such as alteration of gut integrity and increased permeability, inflammation, and metabolite production have been proposed, more scientific evidence is needed to address this issue and thus further studies are needed.
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- 2019
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13. Omega-3 LCPUFA supplement: a nutritional strategy to prevent maternal and neonatal oxidative stress.
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Kajarabille N, Hurtado JA, Peña-Quintana L, Peña M, Ruiz J, Diaz-Castro J, Rodríguez-Santana Y, Martin-Alvarez E, López-Frias M, Soldado O, Lara-Villoslada F, and Ochoa JJ
- Subjects
- Adult, Biomarkers blood, Double-Blind Method, Fatty Acids, Omega-3 blood, Female, Fetal Blood chemistry, Fish Oils administration & dosage, Humans, Infant, Infant, Newborn, Lactation, Male, Pregnancy, Ubiquinone analogs & derivatives, Ubiquinone blood, alpha-Tocopherol blood, Dietary Supplements, Fatty Acids, Omega-3 administration & dosage, Maternal Nutritional Physiological Phenomena, Oxidative Stress drug effects
- Abstract
There is controversy about fish-oil supplementation and oxidative damage. This ambiguity should be explored to elucidate its role as modulator of oxidative stress, especially during gestation and postnatal life. This is the objective of this study. One hundred ten pregnant women were divided in two groups: control group CT (400 mL/day of the control dairy drink); supplemented group FO (400 mL/day of the fish oil-enriched dairy drink (±400-mg EPA-DHA/day)). Different biomarkers of oxidative damage were determined in the mother's at enrolment, at delivery and at 2.5 and 4 months postpartum and newborns at delivery and at 2.5 months postpartum. Omega-3 LC-PUFA supplementation during pregnancy and lactation decreased plasma hydroperoxides especially in newborn at delivery (P = 0.001) and 2.5 months (P = 0.006), increased superoxide dismutase (SOD) and catalase (CAT) in mothers at delivery (P = 0.024 (SOD)) and after 2.5 months (P = 0.040 (CAT)) and in newborns at 2.5 months (P = 0.035 (SOD); P = 0.021 (CAT)). Also, supplementation increased α-tocoferol in mothers at 2.5 months (P = 0.030) and in umbilical cord artery (P = 0.039). Higher levels of CoQ10 were found in mothers at delivery (P = 0.039) as well as in umbilical cord vein (P = 0.024) and artery (P = 0.036). Our supplementation prevents the oxidative stress in the mother and neonate during the first months of postnatal life, being a potential preventive nutritional strategy to prevent functional alterations associated with oxidative stress that have an important repercussion for the neonate development in the early postnatal life., (© 2016 John Wiley & Sons Ltd.)
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- 2017
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14. Bioavailability of iron multi-amino acid chelate preparation in mice and human duodenal HuTu 80 cells.
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Kajarabille N, Brown C, Cucliciu A, Thapaliya G, and Latunde-Dada GO
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- Amino Acids therapeutic use, Anemia, Iron-Deficiency drug therapy, Animals, Ascorbic Acid pharmacology, Biological Availability, Cell Line, Diet, Gene Expression Regulation, Hemoglobins metabolism, Hepcidins metabolism, Humans, Iron metabolism, Iron therapeutic use, Iron Chelating Agents therapeutic use, Iron Deficiencies, Iron, Dietary metabolism, Iron, Dietary therapeutic use, Liver metabolism, Male, Mice, Nutritional Status, Polymerase Chain Reaction, RNA, Messenger metabolism, Spleen metabolism, Amino Acids pharmacology, Anemia, Iron-Deficiency metabolism, Dietary Supplements, Duodenum metabolism, Intestinal Absorption, Iron pharmacokinetics, Iron Chelating Agents pharmacology
- Abstract
Strategies for preventing Fe deficiency include Fe supplementation and Fe fortification of foods. The absorption, metabolism and chemical characteristics of Fe multi-amino acid chelate (IMAAC) are not known. Absorption of IMAAC was compared with FeSO4 in Fe-depleted mice and in vitro chemical studies of the Fe supplement was performed in HuTu 80 cells. Hb repletion study was carried out in Fe-deficient CD1 mice that were fed for 10 d a diet supplemented with ferrous IMAAC or FeSO4. A control group of Fe-replete mice was fed a diet with adequate Fe concentrations throughout the study. Tissues were collected from the mice, and the expression of Fe-related genes was determined by quantitative PCR. Ferric reductase and Fe uptake were evaluated in HuTu 80 cells. Supplementation of the diet with FeSO4 or IMAAC significantly increased Hb levels (P<0·001) in Fe-deficient mice from initial 93·9 (SD 10·8) or 116·2 (SD 9·1) to 191 (SD 0·7) or 200 (SD 0·5) g/l, respectively. Initial and final Hb for the Fe-deficient control group were 87·4 (SD 6·7) and 111 (SD 11·7) g/l, respectively. Furthermore, the liver non-haem Fe of both supplement groups increased significantly (P<0·001). IMAAC was more effective at restoring Fe in the spleen compared with FeSO4 (P<0·005). Gene expression showed the IMAAC supplement absorption is regulated by the body's Fe status as it significantly up-regulated hepcidin (P<0·001) and down-regulated duodenal cytochrome b mRNA (P<0·005), similar to the effects seen with FeSO4. A significant proportion of Fe in IMAAC is reduced by ascorbic acid. Fe absorption in mice and cells was similar for both IMAAC and FeSO4 and both compounds induce and regulate Fe metabolism genes similarly in the maintenance of homeostasis in mice.
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- 2017
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15. Short-term ubiquinol supplementation reduces oxidative stress associated with strenuous exercise in healthy adults: A randomized trial.
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Sarmiento A, Diaz-Castro J, Pulido-Moran M, Moreno-Fernandez J, Kajarabille N, Chirosa I, Guisado IM, Javier Chirosa L, Guisado R, and Ochoa JJ
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- Adult, Dietary Supplements, Drug Administration Schedule, Healthy Volunteers, Humans, Male, Middle Aged, Resistance Training, Ubiquinone administration & dosage, Antioxidants administration & dosage, Oxidative Stress drug effects, Physical Exertion drug effects, Ubiquinone analogs & derivatives
- Abstract
Studies about Coenzyme Q
10 (CoQ10 ) supplementation on strenuous exercise are scarce, especially those related with oxidative stress associated with physical activity and virtually nonexistent with the reduced form, Ubiquinol. The objective of this study was to determine, for the first time, whether a short-term supplementation with Ubiquinol can prevent oxidative stress associated to strenuous exercise. The participants (n = 100 healthy and well trained, but not on an elite level) were classified in two groups: Ubiquinol (experimental group), and placebo group (control). The protocol consisted of conducting two identical strenuous exercise tests with a rest period between tests of 24 h. Blood and urine samples were collected from the participants before supplementation (basal value) (T1), after supplementation (2 weeks) (T2), after first physical exercise test (T3), after 24 h of rest (T4), and after second physical exercise test (T5).The increase observed in the lactate, isoprostanes, DNA damage, and hydroperoxide levels reveals the severity of the oxidative damage induced by the exercise. There was a reduction in the isoprostanes, 8-OHdG, oxidized LDL, and hydroperoxydes in the supplemented Ubiquinol group, an increase in total antioxidant status, fat soluble antioxidant (both plasma and membrane), and CAT activity. Also, NO in the Ubiquinol-supplemented group was maintained within a narrow range. Oxidative stress induced by strenuous exercise is accumulative and increases transiently in subsequent sessions of physical activity. A short-term supplementation (2 weeks) with Ubiquinol (200 mg/day) before strenuous exercise, decreases oxidative stress and increases plasma NO, fact that could improve endothelial function, energetic substrate supply, and muscle recovery after strenuous exercise. © 2016 BioFactors, 42(6):612-622, 2016., (© 2016 International Union of Biochemistry and Molecular Biology.)- Published
- 2016
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16. Gender specific differences in oxidative stress and inflammatory signaling in healthy term neonates and their mothers.
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Diaz-Castro J, Pulido-Moran M, Moreno-Fernandez J, Kajarabille N, de Paco C, Garrido-Sanchez M, Prados S, and Ochoa JJ
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- Adult, Antioxidants metabolism, Catalase blood, Dinoprostone blood, Female, Glutathione Peroxidase blood, Humans, Hydrogen Peroxide blood, Infant, Newborn, Interleukin-6 blood, Male, Mothers, Pregnancy, Signal Transduction, Superoxide Dismutase blood, Tumor Necrosis Factor-alpha blood, Umbilical Arteries metabolism, Umbilical Cord metabolism, Inflammation metabolism, Oxidative Stress, Sex Factors
- Abstract
Background: Gender is a crucial determinant of life span, but little is known about gender differences in free radical homeostasis and inflammatory signaling. The aim of the study was to determine gender-related differences concerning oxidative stress and inflammatory signaling of healthy neonates and mothers., Methods: Fifty-six mothers with normal gestational course and spontaneous delivery were selected. Blood samples were collected from the mother (at the beginning of delivery and start of expulsive period) and from neonate (from umbilical cord vein and artery)., Results: The mothers of girls featured a higher total antioxidant status and lower plasma hydroperoxides than the mother of boys. Regarding the neonates, the girls featured a higher total antioxidant status and lower plasma membrane hydroperoxides in umbilical cord artery together with higher catalase, glutathione peroxidase, and superoxide dismutase activities. Lower levels of interleukin 6, tumor necrosis factor alpha, and prostaglandin E2 were observed in the mothers of girls and higher level of soluble tumor necrosis factor receptor II. In the neonates, lower levels of interleukin 6 and tumor necrosis factor alpha were observed in umbilical artery and higher soluble tumor necrosis factor receptor II in umbilical cord vein and artery of girls., Conclusion: An association between gender, oxidative stress, and inflammation signaling exists, leading to a renewed interest in the neonate's sex as a potential risk factor to several alterations.
- Published
- 2016
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17. [In Process Citation].
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Martín Álvarez E, Jiménez Cabanillas MV, Peña Caballero M, Serrano López L, Kajarabille N, Díaz Castro J, Ochoa Herrera JJ, and Maldonado Lozano J
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- Female, Humans, Infant, Infant, Newborn, Male, Milk, Human chemistry, Colostrum, Immunoglobulin A blood, Infant, Premature immunology, Infant, Very Low Birth Weight immunology, Oropharynx
- Abstract
Introducción: el recién nacido prematuro de muy bajo peso (RNMBP) es inmunológicamente inmaduro y además presenta una alteración de las barreras naturales de defensa. Objetivo: evaluar los efectos que pueda tener la administración de calostro orofaríngeo, administrado durante los primeros 15 días posnatales, sobre los niveles de inmunoglobulina A (IgA) sérica en recién nacidos prematuros de muy bajo peso durante el primer mes de vida. Material y métodos: se desarrolló un estudio de intervención no aleatorizado con grupo control, en el que se incluyeron 38 recién nacidos con ≤ 32 + 6 semanas de gestación y/o menores de 1.500 g de peso. Los sujetos recibieron 0,2 ml de calostro de su madre cada 4 h, iniciándose el procedimiento en las primeras 24 h de vida hasta el 15.o día postnatal. Se midieron los niveles de IgA en la sangre al nacimiento, 3. er , 15.o y 30.o días de vida. Se registraron datos perinatales al nacimiento y durante el periodo de seguimiento. Resultados: IgA sérica aumentó de forma estadísticamente significativa en el grupo de intervención (M1 15,84 μg/ml, M2 20,07 μg/ml, M3 23,65 μg/ml, M4 30,34 μg/ml, p 0,001) y en el grupo control (M1 12,48 μg/ml, M2 16,48 μg/ml, p 0,018; M3 19,41 μg/ml, M4 22,48 μg/ml, p 0,001). Al mes de vida, los niveles de IgA sérica fueron significativamente mayores en el grupo de intervención que en el grupo control (p 0,026). Conclusiones: este estudio sugiere que la administración de calostro orofarínge.
- Published
- 2016
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18. Coenzyme Q10 Supplementation and Exercise in Healthy Humans: A Systematic Review.
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Sarmiento A, Diaz-Castro J, Pulido-Moran M, Kajarabille N, Guisado R, and Ochoa JJ
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- Antioxidants administration & dosage, Antioxidants analysis, Exercise Tolerance drug effects, Humans, Non-Randomized Controlled Trials as Topic, Oxidative Stress drug effects, Randomized Controlled Trials as Topic, Ubiquinone administration & dosage, Ubiquinone blood, Ubiquinone pharmacology, Vitamins blood, Antioxidants pharmacology, Dietary Supplements, Energy Metabolism drug effects, Exercise, Ubiquinone analogs & derivatives, Vitamins pharmacology
- Abstract
Objective: Coenzyme Q10 (CoQ10) is an endogenous lipid-soluble benzoquinone compound that functions as a diffusible electron carrier in the electron transport chain. It is prevalent in all human tissues and organs, although it is mainly biosynthesised and concentrated in tissues with high energy turnover. The aim of this review was to perform an exhaustive analysis of the influence and effects of CoQ10 supplementation on parameters related to exercise in healthy humans, and to clarify the current state of knowledge of this field of study, presenting the relevant data in a systematic manner., Method: This paper describes a transversal descriptive systematic review of published research in this field; the study was conducted using a method adapted from the PRISMA guidelines. The inclusion criteria applied were based on the PICO (population, intervention, comparison, and outcome) model., Results: The database search performed yielded 372 citations. Finally, 13 studies met all the inclusion criteria and were incorporated in the present review., Conclusion: CoQ10 has properties related to bioenergetic and antioxidant activity; thus, it is intimately involved in energy production and in the prevention of peroxidative damage to membrane phospholipids and of free radical-induced oxidation. These properties make it suitable as a dietary supplement to improve cellular bioenergetics and to inhibit certain age-related pathologies.
- Published
- 2016
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19. Effects of Maternal Ω-3 Supplementation on Fatty Acids and on Visual and Cognitive Development.
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Hurtado JA, Iznaola C, Peña M, Ruíz J, Peña-Quintana L, Kajarabille N, Rodriguez-Santana Y, Sanjurjo P, Aldámiz-Echevarría L, Ochoa J, and Lara-Villoslada F
- Subjects
- Beverages, Cognition Disorders blood, Cognition Disorders metabolism, Cognition Disorders prevention & control, Colostrum chemistry, Dairy Products, Double-Blind Method, Evoked Potentials, Visual, Fatty Acids analysis, Fatty Acids blood, Fatty Acids metabolism, Fatty Acids, Omega-3 administration & dosage, Fatty Acids, Omega-3 analysis, Fatty Acids, Omega-3 metabolism, Female, Fish Oils administration & dosage, Fish Oils metabolism, Fish Oils therapeutic use, Humans, Infant, Newborn, Male, Milk, Human chemistry, Placenta metabolism, Pregnancy, Vision Disorders blood, Vision Disorders metabolism, Vision Disorders prevention & control, Child Development, Fatty Acids, Omega-3 therapeutic use, Fetal Development, Food, Fortified, Lactation, Maternal Nutritional Physiological Phenomena, Neurogenesis
- Abstract
Objectives: The aim of the present study was to elucidate whether a dairy drink enriched with ω-3 long-chain polyunsaturated fatty acid (LC-PUFA) could have an impact on the lipid profile of the mother and the newborn, and also whether this intervention could affect the newborns' visual and cognitive development., Methods: A total of 110 pregnant women were randomly assigned to one of the following intervention groups: control group (n = 54), taking 400 mL/day of the control dairy drink, and supplemented group (fish oil [FO]) (n = 56), taking 400 mL/day of the fish oil-enriched dairy drink (including ∼400 mg eicosapentaenoic acid-docosahexaenoic acid [DHA]/day). During the study, the mothers' diets were supervised by a nutritionist to encourage compliance with present recommendations of FA intake. Blood fatty acid profiles were determined in the mother's (at enrollment, at delivery, and at 2.5 and 4 months) and newborn (at delivery and at 2.5 months) placenta and breast milk (colostrum and at 1, 2, and 4 months). Pattern reversal visual evoked potentials (VEPs) (at 2.5 and 7.5 months) and Bayley test (at 12 months) were recorded., Results: DHA percentage was higher in plasma, erythrocyte membranes, and breast milk samples from the FO group. The ratio of nervonic acid was also higher in plasma and erythrocyte lipids of the mother and newborn's blood samples from the FO group. No differences were observed in the Bayley test. No differences were observed in VEPs between both groups. We observed a shorter latency, however, in the lower visual angle (7.5') in the boys of the supplemented group., Conclusions: Omega-3 LC-PUFA dietary supplement during pregnancy and lactation influenced the mother and newborn's fatty acid profile and nervonic acid content but did not show effects on visual and cognitive/psychomotor development.
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- 2015
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20. A new approach to oxidative stress and inflammatory signaling during labour in healthy mothers and neonates.
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Díaz-Castro J, Florido J, Kajarabille N, Prados S, de Paco C, Ocon O, Pulido-Moran M, and Ochoa JJ
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- Adult, Catalase metabolism, Dinoprostone blood, Female, Fetal Blood metabolism, Gestational Age, Glutathione Peroxidase metabolism, Humans, Infant, Newborn, Mothers, Pregnancy, Signal Transduction, Superoxide Dismutase metabolism, Interleukin-6 metabolism, Labor, Obstetric, Oxidative Stress, Tumor Necrosis Factor-alpha metabolism
- Abstract
The objective of the current study was to investigate for the first time and simultaneously the oxidative stress and inflammatory signaling induced during the delivery in healthy mothers and their neonates. 56 mothers with normal gestational course and spontaneous delivery were selected. Blood samples were taken from mother (before and after delivery) both from vein and artery of umbilical cord. Lower antioxidant enzymes activities were observed in neonates compared with their mothers and lower oxidative stress in umbilical cord artery with respect to vein. There was an overexpression of inflammatory cytokines in the mother, such as IL-6 and TNF-α, and, in addition, PGE2 was also increased. Neonates showed lower levels of IL-6 and TNF-α and higher values of sTNF-RII and PGE2 in comparison with their mothers. Parturition increases oxidative damage in the mother, although the indicators of oxidative damage were lower in umbilical cord artery with respect to umbilical vein. The overexpression of inflammatory cytokines reveals that fetus suffers its own inflammatory process during parturition.
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- 2015
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21. The timing of cord clamping and oxidative stress in term newborns.
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Díaz-Castro J, Florido J, Kajarabille N, Garrido-Sánchez M, Padilla C, de Paco C, Navarrete L, and Ochoa JJ
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- Catalase blood, Constriction, Pathologic, Erythrocytes enzymology, Female, Humans, Inflammation Mediators blood, Interleukin-6 blood, Labor Stage, Third physiology, Ligation standards, Placental Circulation physiology, Postpartum Hemorrhage prevention & control, Pregnancy, Reactive Oxygen Species metabolism, Superoxide Dismutase blood, Term Birth, Time Factors, Tumor Necrosis Factor-alpha blood, Infant, Newborn physiology, Oxidative Stress physiology, Pregnancy Outcome, Umbilical Cord blood supply, Umbilical Cord surgery
- Abstract
Background: Clamping and cutting of the umbilical cord is the most prevalent of all operations, but the optimal timing of cord clamping is controversial, with different timings offering advantages and disadvantages. This study, for the first time, compares the influence of early and late cord clamping in correlation with oxidative stress and inflammation signaling, Because cord clamping timing may have a significant influence on placenta-to-infant blood transfer, thereby modifying oxygenation of maternal and fetal tissues, and on the transfer of inflammatory mediators throughout the placenta., Methods: Sixty-four pregnant subjects were selected at the Gynecology and Obstetrics Services Department of the Clinico San Cecilio Hospital, Granada, Spain, based on disease-free women who experienced a normal course of pregnancy and a spontaneous, vaginal, single delivery. Half of the subjects had deliveries with early-clamped newborn infants (at 10 s), and the other half had late-clamped deliveries (at 2 min)., Results: Erythrocyte catalase activity was significantly greater in the late-clamped group than in the early-clamped group (P < .01 for the umbilical vein and P < .001 for the artery). The values for superoxide dismutase, total antioxidant status, and soluble tumor necrosis factor receptor II were all significantly higher in the late-clamped group compared with the early-clamped group (P < .01, P < .001, and P < .001, respectively)., Conclusions: The results suggest a beneficial effect of late cord clamping, produced by an increase in antioxidant capacity and moderation of the inflammatory-mediated effects induced during delivery of term neonates., (Copyright © 2014 by the American Academy of Pediatrics.)
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- 2014
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22. A new insight to bone turnover: role of ω-3 polyunsaturated fatty acids.
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Kajarabille N, Díaz-Castro J, Hijano S, López-Frías M, López-Aliaga I, and Ochoa JJ
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- Aging metabolism, Animals, Bone Remodeling drug effects, Bone and Bones drug effects, Bone and Bones metabolism, Fatty Acids, Omega-3 pharmacology, Female, Humans, Inflammation metabolism, Inflammation pathology, Male, Nutritional Physiological Phenomena, Obesity metabolism, Obesity pathology, Osteoblasts metabolism, Osteoclasts metabolism, Oxidative Stress, Signal Transduction, Bone Remodeling physiology, Fatty Acids, Omega-3 metabolism
- Abstract
Background. Evidence has shown that long-chain polyunsaturated fatty acids (LCPUFA), especially the ω -3 fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are beneficial for bone health and turnover. Objectives. This review summarizes findings from both in vivo and in vitro studies and the effects of LC PUFA on bone metabolism, as well as the relationship with the oxidative stress, the inflammatory process, and obesity. Results. Some studies in humans indicate that LCPUFA can increase bone formation, affect peak bone mass in adolescents, and reduce bone loss. However, the cellular mechanisms of action of the LCPUFA are complex and involve modulation of fatty acid metabolites such as prostaglandins, resolvins and protectins, several signaling pathways, cytokines, and growth factors, although in certain aspects there is still some controversy. LCPUFA affect receptor activator of nuclear factor κ β (RANK), a receptor found on the osteoclast, causing bone resorption, which controls osteoclast formation. Conclusions. Since fatty acids are an endogenous source of reactive oxygen species, free radicals alter the process of bone turnover; however, although there are clinical evidences linking bone metabolism and dietary lipids, more clinical trials are necessary to prove whether ω -3 PUFA supplementation plays a major role in bone health.
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- 2013
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23. Coenzyme Q(10) supplementation ameliorates inflammatory signaling and oxidative stress associated with strenuous exercise.
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Díaz-Castro J, Guisado R, Kajarabille N, García C, Guisado IM, de Teresa C, and Ochoa JJ
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- 8-Hydroxy-2'-Deoxyguanosine, Administration, Oral, Adult, Antioxidants metabolism, Athletes, Catalase metabolism, Deoxyguanosine analogs & derivatives, Deoxyguanosine blood, Humans, Inflammation Mediators antagonists & inhibitors, Inflammation Mediators metabolism, Interleukin-6 blood, Isoprostanes blood, Male, Signal Transduction drug effects, Tumor Necrosis Factor-alpha blood, Ubiquinone administration & dosage, Dietary Supplements, Inflammation drug therapy, Oxidative Stress drug effects, Running physiology, Ubiquinone analogs & derivatives
- Abstract
Background: Exhausting exercise induces muscle damage associated with high production of free radicals and pro-inflammatory mediators., Aim: The objective of this study was to determine for the first time and simultaneously whether oral coenzyme Q(10) (CoQ(10)) supplementation can prevent over-expression of inflammatory mediators and oxidative stress associated with strenuous exercise., Methods: The participants were classified in two groups: CoQ(10) group (CG) and placebo group (PG). The physical test consisted in a constant run (50 km) that combined several degrees of high effort (mountain run and ultra-endurance), in permanent climbing., Results: Exercise was associated with an increase in TNF-α, IL-6, 8-hydroxy-2'-deoxyguanosine (8-OHdG), and isoprostane levels, revealing the degree of inflammation and oxidative stress induced. Oral supplementation of CoQ(10) during exercise was efficient reducing oxidative stress (decreased membrane hydroperoxides, 8-OHdG and isoprostanes generation, increased catalase, and total antioxidant status), which would lead to the maintenance of the cell integrity. Data obtained also indicate that CoQ(10) prevents over-expression of TNF-α after exercise, together with an increase in sTNF-RII that limits the pro-inflammatory actions of TNF. Moreover, CoQ(10) supplementation reduced creatinine production., Conclusions: CoQ(10) supplementation before strenuous exercise decreases the oxidative stress and modulates the inflammatory signaling, reducing the subsequent muscle damage.
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- 2012
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24. Phlebodium decumanum is a natural supplement that ameliorates the oxidative stress and inflammatory signalling induced by strenuous exercise in adult humans.
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Díaz-Castro J, Guisado R, Kajarabille N, García C, Guisado IM, De Teresa C, and Ochoa JJ
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- 8-Hydroxy-2'-Deoxyguanosine, Adult, Anti-Inflammatory Agents isolation & purification, Antioxidants isolation & purification, Biomarkers blood, Biomarkers urine, Catalase blood, Deoxyguanosine analogs & derivatives, Deoxyguanosine urine, Exercise Test, Glutathione Peroxidase blood, Humans, Interleukin 1 Receptor Antagonist Protein blood, Interleukin-6 blood, Isoprostanes urine, Muscle, Skeletal immunology, Muscle, Skeletal metabolism, Physical Endurance, Plant Extracts isolation & purification, Plant Leaves, Receptors, Tumor Necrosis Factor, Type II blood, Rhizome, Running, Spain, Tumor Necrosis Factor-alpha blood, Anti-Inflammatory Agents pharmacology, Antioxidants pharmacology, Dietary Supplements, Exercise, Inflammation Mediators blood, Muscle, Skeletal drug effects, Oxidative Stress drug effects, Plant Extracts pharmacology, Polypodiaceae chemistry, Signal Transduction drug effects
- Abstract
Strenuous exercise induces muscle damage due to a highly increased generation of free radicals and inflammatory response and therefore, in this type of exercise, it is important to reduce both oxidative stress and inflammation, at least their negative aspects. The purpose of this study was investigate, for the first time, whether a purified, standard water-soluble fraction obtained from Phlebodium decamanum could reduce the over-expression of inflammation and oxidative stress induced by strenuous exercise. The physical test consisted of a constant run that combined several degrees of high effort (mountain run and ultra-endurance), in permanent climbing. Biochemical parameters, oxidative stress and inflammatory mediators were assessed. The results showed that oral supplementation of P. decumanum during high-intensity exercise effectively reduces the degree of oxidative stress (decreased 8-hydroxy-2'-deoxyguanosine and isoprostanes generation, increased antioxidant enzyme activities in erythrocyte and total antioxidant status in plasma). The data obtained also indicate that this supplementation is efficient in reducing the inflammatory response through the decrease of TNF-α and increase of sTNF-RII, but kept the levels of IL-6 and IL-1ra. In conclusion, oral supplementation of P. decamanum extract during high-intensity exercise effectively reduces the degree of oxidative stress and has anti-inflammatory protective effects, preventing the over-expression of TNF-α but keeping the levels and effects of IL-6. These findings provide a basis for similar Phlebodium supplementation for both professional and amateur athletes performing strenuous exercise in order to reduce the undesirable effects of the oxidative stress and inflammation signalling elicited during high-intensity exercise.
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- 2012
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25. Melatonin supplementation ameliorates oxidative stress and inflammatory signaling induced by strenuous exercise in adult human males.
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Ochoa JJ, Díaz-Castro J, Kajarabille N, García C, Guisado IM, De Teresa C, and Guisado R
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- 8-Hydroxy-2'-Deoxyguanosine, Deoxyguanosine analogs & derivatives, Deoxyguanosine urine, Dietary Supplements, Humans, Inflammation blood, Inflammation etiology, Interleukin 1 Receptor Antagonist Protein blood, Interleukin-6 blood, Isoprostanes urine, Male, Tumor Necrosis Factor-alpha blood, Exercise physiology, Inflammation drug therapy, Melatonin therapeutic use, Oxidative Stress drug effects
- Abstract
Strenuous exercise induces inflammatory reactions together with high production of free radicals and subsequent muscle damage. This study was designed to investigate for the first time and simultaneously whether over-expression of inflammatory mediators, oxidative stress, and alterations in biochemical parameters induced by acute exercise could be prevented by melatonin. This indoleamine is a potent, endogenously produced free radical scavenger and a broad-spectrum antioxidant; consequently, it might have positive effects on the recovery following an exercise session. The participants were classified into two groups: melatonin-treated men (MG) and placebo-treated individuals (controls group, CG). The physical test consisted in a constant run that combined several degrees of high effort (mountain run and ultra-endurance). The total distance of the run was 50 km with almost 2800 m of ramp in permanent climbing and very changeable climatic conditions. Exercise was associated with a significant increase in TNF-α, IL-6, IL-1ra (in blood), and also an increase in 8-hydroxy-2'-deoxyguanosine (8-OHdG) and isoprostane levels (in urine), and indicated the degree of oxidative stress and inflammation induced. Oral supplementation of melatonin during high-intensity exercise proved efficient in reducing the degree of oxidative stress (lower levels of lipid peroxidation, with a significant increase in antioxidative enzyme activities); this would lead to the maintenance of the cellular integrity and reduce secondary tissue damage. Data obtained also indicate that melatonin has potent protective effects, by preventing over-expression of pro-inflammatory mediators and inhibiting the effects of several pro-inflammatory cytokines. In summary, melatonin supplementation before strenuous exercise reduced muscle damage through modulation of oxidative stress and inflammation signaling associated with this physical challenge., (© 2011 John Wiley & Sons A/S.)
- Published
- 2011
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