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1. Quantification of short and long asbestos fibers to assess asbestos exposure: a review of fiber size toxicity

Author: Annie Sobaszek, Pascal Dumortier, Chantal Dion, Jean Claude Pairon, Christophe Paris, Marie Claude Jaurand, Pierre Bartsch, Pascal Andujar, Marie Annick Billon-Galland, Patrick Brochard, Guillaume Boulanger, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service Pneumologie et Pathologie Professionnelle, CHI Créteil, Direction de l'Action Sociale de l'Enfance et de la Santé (DASES), Mairie de Paris, Institut de Recherche Robert-Sauvé en Santé et en Sécurité du Travail ((IRSST)), Université du Québec à Montréal = University of Québec in Montréal (UQAM)-faculté de Sciences Humaines, Département de Santé Environnementale et Santé au Travail ((DSEST)), École de santé publique, Hôpital Erasme [Bruxelles] (ULB), Faculté de Médecine [Bruxelles] (ULB), Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB), Laboratoire Santé Travail Environnement, Université Bordeaux Segalen - Bordeaux 2-IFR99, Médecine de Travail et Pathologies Professionnelles, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Service Pneumologie, Université de Liège-CHU Liège, Nutrition-Génétique et Exposition aux Risques Environnementaux (NGERE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Génomique Fonctionnelle des Tumeurs Solides (U1162), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), Agence Nationale de Sécurité Sanitaire, de l'Alimentation, de l'environnement et du Travail (ANSES), ANSES, Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12) - Institut National de la Santé et de la Recherche Médicale (INSERM) - IFR10, Faculté de Médecine, Hôpital Henri Mondor - Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de Pneumologie et Pathologie Professionnelle, CHI Créteil - Faculté de Médecine, Laboratoire d'Etude des Particules Inhalées, Ville de Paris - DASES, Département de santé environnementale et de santé au travail, Université de Montréal (UdeM), Institut de recherche Robert-Sauvé en santé et en sécurité du travail (IRSST), Institut de recherche Robert-Sauvé en santé et en sécurité du travail, Hôpital Erasme, Université Libre de Bruxelles [Bruxelles] (ULB), Université Bordeaux Segalen - Bordeaux 2 - IFR99, Université de Lille, Droit et Santé, Université de Liège - CHU Liège, Université Nancy 2, Institut National de la Santé et de la Recherche Médicale (INSERM) - Université de Lorraine (UL), Université Paris 13 (UP13) - Université Paris Diderot - Paris 7 (UPD7) - Université Paris Descartes - Paris 5 (UPD5) - Institut National de la Santé et de la Recherche Médicale (INSERM), Agence Nationale de Sécurité Sanitaire, de l'Alimentation, de l'environnement et du Travail ( ANSES ), ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail ( ANSES ), Institut Mondor de Recherche Biomédicale ( IMRB ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 ), Institut de Recherche Robert-Sauvé en Santé et en Sécurité du Travail ( (IRSST) ), Université du Québec à Montréal ( UQAM ) -faculté de Sciences Humaines, Département de Santé Environnementale et Santé au Travail ( (DSEST) ), Université de Montréal-École de santé publique, Université de Bruxelles, Centre Hospitalier Régional Universitaire [Lille] ( CHRU Lille ), CHU Liège-Université de Liège, Nutrition-Génétique et Exposition aux Risques Environnementaux ( NGERE ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université de Lorraine ( UL ), Genomique Fonctionnelle des Tumeurs Solides, Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ) -IFR105-Université Paris Diderot - Paris 7 ( UPD7 ), Université Paris Descartes - Paris 5 ( UPD5 ), and Autard, Delphine
Subjects: Health, Toxicology and Mutagenesis, Air pollution, [SDV.GEN] Life Sciences [q-bio]/Genetics, Review, medicine.disease_cause, Santé publique, World health, Asbestos, Asbestos fibers, medicine, Animals, Humans, Fiber, Health risk, Inhalation exposure, Air Pollutants, Inhalation Exposure, [SDV.GEN]Life Sciences [q-bio]/Genetics, Mutagenèse et technologie génétique, Toxicity, Public Health, Environmental and Occupational Health, Fiber size, Environmental exposure, Particle size, Occupational exposure, Pulp and paper industry, 3. Good health, [SDV.TOX] Life Sciences [q-bio]/Toxicology, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Environmental science, [ SDV.GEN ] Life Sciences [q-bio]/Genetics
Abstract: The fibrogenicity and carcinogenicity of asbestos fibers are dependent on several fiber parameters including fiber dimensions. Based on the WHO (World Health Organization) definition, the current regulations focalise on long asbestos fibers (LAF) (Length: L ≥ 5 μm, Diameter: D < 3 μm and L/D ratio > 3). However air samples contain short asbestos fibers (SAF) (L < 5 μm). In a recent study we found that several air samples collected in buildings with asbestos containing materials (ACM) were composed only of SAF, sometimes in a concentration of ≥10 fibers.L-1. This exhaustive review focuses on available information from peer-review publications on the size-dependent pathogenetic effects of asbestos fibers reported in experimental in vivo and in vitro studies. In the literature, the findings that SAF are less pathogenic than LAF are based on experiments where a cut-off of 5 μm was generally made to differentiate short from long asbestos fibers. Nevertheless, the value of 5 μm as the limit for length is not based on scientific evidence, but is a limit for comparative analyses. From this review, it is clear that the pathogenicity of SAF cannot be completely ruled out, especially in high exposure situations. Therefore, the presence of SAF in air samples appears as an indicator of the degradation of ACM and inclusion of their systematic search should be considered in the regulation. Measurement of these fibers in air samples will then make it possible to identify pollution and anticipate health risk. © 2014 Boulanger et al. licensee BioMed Central Ltd., SCOPUS: re.j, info:eu-repo/semantics/published
Published: 2014

2. Intracellular fate of carbon nanotubes inside murine macrophages: pH-dependent detachment of iron catalyst nanoparticles

Author: Julien Cambedouzou, Mathieu Pinault, Cyrill Bussy, Mickaël Huard, C. Mory, Sophie Lanone, Murielle Salomé, Jorge Boczkowski, Esther Belade, Pascale Launois, Barbara Fayard, Nathalie Brun, Erwan Paineau, Lucie Armand, INSERM U955, équipe 4, Laboratoire de Physique des Solides (LPS), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Nanomedicine laboratory, University College of London [London] (UCL)-Centre for Drug Delivery Research-UCL School of Pharmacy-University College of London [London] (UCL)-Centre for Drug Delivery Research-UCL School of Pharmacy-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11), Institut de Chimie Séparative de Marcoule (ICSM - UMR 5257), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), X-ray Imaging Group, European Synchrotron Radiation Facility (ESRF), Laboratoire Francis PERRIN (LFP - URA 2453), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de Physiologie Explorations Fonctionnelles, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Service de pneumologie et pathologie professionnelle, Centre Hospitalier Intercommunal de Créteil (CHIC)-Centre Hospitalier Intercommunal de Créteil (CHIC)-Institut Mondor de Recherche Biomédicale (IMRB), Service de pneumologie et pathologie professionnelle, CB and EP respectively acknowledge C'Nano Ile de France and RTRA 'Triangle de la Physique' for financial support of their fellowships. C'Nano-IdF is the nanoscience competence center of Paris Region, supported by CNRS, CEA, MESR, and Région Ile-de-France., BMC, Ed., Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Nanomedicine laboratory, Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials science, Health, Toxicology and Mutagenesis, Carbon nanotubes, Metal Nanoparticles, Nanoparticle, Nanotechnology, Spectroscopy, Electron Energy-Loss, 02 engineering and technology, Carbon nanotube, Toxicology, Cathepsin B, Cell Line, law.invention, Nanomaterials, Catalysis, Mice, 03 medical and health sciences, Degradation, Microscopy, Electron, Transmission, X-Ray Diffraction, law, Animals, 030304 developmental biology, 0303 health sciences, Toxicity, Nanotubes, Carbon, Research, Macrophages, Electron energy loss spectroscopy, Lysosome-Associated Membrane Glycoproteins, Spectrometry, X-Ray Emission, General Medicine, Hydrogen-Ion Concentration, 021001 nanoscience & nanotechnology, Fluorescence, Lysosome acidification, Iron catalyst nanoparticles, Transmission electron microscopy, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Biophysics, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Macrolides, Lysosomes, 0210 nano-technology, Iron Compounds, Synchrotrons, Intracellular
Abstract: Background Carbon nanotubes (CNT) are a family of materials featuring a large range of length, diameter, numbers of walls and, quite often metallic impurities coming from the catalyst used for their synthesis. They exhibit unique physical properties, which have already led to an extensive development of CNT for numerous applications. Because of this development and the resulting potential increase of human exposure, an important body of literature has been published with the aim to evaluate the health impact of CNT. However, despite evidences of uptake and long-term persistence of CNT within macrophages and the central role of those cells in the CNT-induced pulmonary inflammatory response, a limited amount of data is available so far on the CNT fate inside macrophages. Therefore, the overall aim of our study was to investigate the fate of pristine single walled CNT (SWCNT) after their internalization by macrophages. Methods To achieve our aim, we used a broad range of techniques that aimed at getting a comprehensive characterization of the SWCNT and their catalyst residues before and after exposure of murine macrophages: X-ray diffraction (XRD), High Resolution (HR) Transmission Electron Microscopy (TEM), High Angle Annular Dark Field-Scanning TEM (HAADF-STEM) coupled to Electron Energy Loss Spectroscopy (EELS), as well as micro-X-ray fluorescence mapping (μXRF), using synchrotron radiation. Results We showed 1) the rapid detachment of part of the iron nanoparticles initially attached to SWCNT which appeared as free iron nanoparticles in the cytoplasm and nucleus of CNT-exposed murine macrophages, and 2) that blockade of intracellular lysosomal acidification prevented iron nanoparticles detachment from CNT bundles and protected cells from CNT downstream toxicity. Conclusions The present results, while obtained with pristine SWCNT, could likely be extended to other catalyst-containing nanomaterials and surely open new ways in the interpretation and understanding of CNT toxicity.
Published: 2013

3. Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity

Author: Ari Setyan, Lyes Tabet, Jorge Boczkowski, Michel J. Rossi, Angélique Simon-Deckers, Cyrill Bussy, Sophie Lanone, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Department of Environmental Toxicology, University of California, Institut universitaire romand de Santé au Travail (Institute for Work and Health), Université de Genève (UNIGE)-Université de Lausanne (UNIL), Laboratory of Atmospheric Chemistry [Paul Scherrer Institute] (LAC), Paul Scherrer Institute (PSI), Service de pneumologie et pathologie professionnelle, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor, Pneumologie et Pathologie Professionnelle, CHI Créteil, Lyes Tabet was a recipient of a joint grant from ADEME (Agence de l'Environnement et de la Maitrîse de l'Énergie) and ARKEMA., BMC, Ed., Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), University of California (UC), and Université de Lausanne = University of Lausanne (UNIL)-Université de Genève = University of Geneva (UNIGE)
Subjects: Male, Pulmonary toxicity, Health, Toxicology and Mutagenesis, Biocompatible Materials, 02 engineering and technology, Toxicology, MESH: Occupational Exposure, law.invention, chemistry.chemical_compound, Mice, Coating, MESH: Biocompatible Materials, law, MESH: Animals, Cytotoxicity, Lung, chemistry.chemical_classification, 0303 health sciences, Inhalation Exposure, Mice, Inbred BALB C, MESH: Oxidative Stress, MESH: Polystyrenes, General Medicine, Polymer, 021001 nanoscience & nanotechnology, Occupational Diseases, MESH: Cell Survival, Toxicity, MESH: Nanotubes, Carbon, MESH: Microscopy, Electron, Transmission, MESH: Inhalation Exposure, 0210 nano-technology, MESH: Occupational Diseases, Materials science, Cell Survival, Acute Lung Injury, lcsh:Industrial hygiene. Industrial welfare, MESH: Mice, Inbred BALB C, Nanotechnology, Carbon nanotube, engineering.material, 03 medical and health sciences, Microscopy, Electron, Transmission, In vivo, lcsh:RA1190-1270, Occupational Exposure, Intubation, Intratracheal, Animals, MESH: Lung, Nanotubes, Carbon, Polystyrenes, MESH: Mice, 030304 developmental biology, lcsh:Toxicology. Poisons, MESH: Intubation, Intratracheal, Research, Macrophages, MESH: Acute Lung Injury, MESH: Macrophages, MESH: Male, Oxidative Stress, chemistry, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, engineering, Biophysics, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Polystyrene, lcsh:HD7260-7780.8
Abstract: Background carbon nanotubes (CNT) can have adverse effects on health. Therefore, minimizing the risk associated with CNT exposure is of crucial importance. The aim of this work was to evaluate if coating multi-walled CNT (MWCNT) with polymers could modify their toxicity, thus representing a useful strategy to decrease adverse health effects of CNT. We used industrially-produced MWCNT uncoated (NT1) or coated (50/50 wt%) with acid-based (NT2) or polystyrene-based (NT3) polymer, and exposed murine macrophages (RAW 264.7 cell line) or Balb/c mice by intratracheal administration. Biological experiments were performed both in vitro and in vivo, examining time- and dose-dependent effects of CNT, in terms of cytotoxicity, expression of genes and proteins related to oxidative stress, inflammation and tissue remodeling, cell and lung tissue morphology (optical and transmission electron microscopy), and bronchoalveolar lavage fluid content analysis. Results extensive physico-chemical characterization of MWCNT was performed, and showed, although similar dimensions for the 3 MWCNT, a much smaller specific surface area for NT2 and NT3 as compared to NT1 (54.1, 34 and 227.54 m2/g respectively), along with different surface characteristics. MWCNT-induced cytotoxicity, oxidative stress, and inflammation were increased by acid-based and decreased by polystyrene-based polymer coating both in vitro in murine macrophages and in vivo in lung of mice monitored for 6 months. Conclusions these results demonstrate that coating CNT with polymers, without affecting their intrinsic structure, may constitute a useful strategy for decreasing CNT toxicity, and may hold promise for improving occupational safety and that of general the user.
Published: 2011

4. Exposition précoce à la pollution atmosphérique

Author: Lanone, Sophie, INSERM U955, équipe 4, Service de Physiologie Explorations Fonctionnelles, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Service de pneumologie et pathologie professionnelle, Centre Hospitalier Intercommunal de Créteil (CHIC)-Centre Hospitalier Intercommunal de Créteil (CHIC)-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), and PNR EST
Subjects: aérosol, monoxyde de carbone, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, changement climatique, exposition maternelle, appareil respiratoire, pollution atmosphérique, autophagie, chambre simulation, particule fine, souris, stress oxydant, ozone, gestation, synergie, inflammation, oxyde azote, vulnérabilité, qualité air, dioxyde de soufre
Abstract: National audience; À travers la simulation d’atmosphères réalistes complexes, l’objectif principal du projet PolluHealth est d’évaluer les effets respiratoires, sur la descendance, d’une exposition maternelle à la pollution atmosphérique pendant la gestation. Notre étude originale vise à : 1.Générer et caractériser trois types d’atmosphères polluées complexes (Paris, Pékin et une ville méditerranéenne du futur) / 2.Évaluer les conséquences respiratoires de l’exposition de souris gestantes à ces trois types d’atmosphères sur leur descendance.
Published: 2019

5. Cancer naso-sinusien et expositions professionnelles : données du Réseau national de vigilance et de prévention des pathologies professionnelles (RNV3P) 2001–2016

Author: Nathalie Nourry, Serge Brunel, Vincent Bonneterre, Bénédicte Clin, Catherine Nisse, Juliette Bloch, Quentin Durand-Moreau, Fabrice Herin, Christophe Paris, Murielle Gain, Natalie Vongmany, Lynda Bensefa-Colas, Lynda Larabi, Isabelle Baldi, Isabelle Vanrullen, Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps (LEASP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de consultations de pathologies professionnelles [CHU Toulouse], CHU Toulouse [Toulouse], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU-Hôpitaux de Rouen, CCPPE, Cancer environnement (EPICENE ), Bordeaux population health (BPH), Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Environnement et Prédiction de la Santé des Populations (TIMC-IMAG-EPSP), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre Hospitalier Universitaire [Grenoble] (CHU), Hôpital Côte de Nacre [CHU Caen], CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Hôpital Morvan - CHRU de Brest (CHU - BREST ), Service de Pneumologie et Pathologie Professionnelle, CHI Créteil-Faculté de Médecine, Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), and Les Hôpitaux Universitaires de Strasbourg (HUS)
Subjects: 030506 rehabilitation, 03 medical and health sciences, 0302 clinical medicine, [SDV]Life Sciences [q-bio], Public Health, Environmental and Occupational Health, RNV3P, Cancer naso-sinusien, 0305 other medical science, 030210 environmental & occupational health, Facteurs professionnels, ComputingMilieux_MISCELLANEOUS, 3. Good health
Abstract: Introduction L’exposition a l’amiante est largement decrite comme facteur de risque du cancer du larynx dans la litterature et classe par le CIRC comme cancerogene de groupe 1 depuis 2009. Toutefois, il n’existe en France aucun tableau de reconnaissance de maladie professionnelle pour cette pathologie dans le regime general ou agricole. D’autres facteurs de risque professionnels sont suspectes (brouillards d’acide fort inorganiques, hydrocarbures aromatiques polycycliques). L’action 12.4 du plan cancer 2014–2019 vise l’amelioration des connaissances en cancerologie professionnelle et ceci en exploitant notamment les donnees du reseau national de vigilance et de prevention des pathologies professionnelles (rnv3p) dans ce domaine. L’objectif de cette etude est une description des situations professionnelles a risque de cancer de larynx recenses dans le rnv3p de 2001 a 2016. Methodes Une extraction des cas de cancer du larynx (Codes CIM-10 C32 et D02.0) entre 2001 et 2016 dans la base du rnv3p a ete realisee. Les parametres etudies sont les donnees demographiques, les expositions professionnelles, le poste de travail, le secteur d’activite. Nous avons conserve pour notre etude les cas de cancer du larynx dont l’exposition est consideree par le medecin expert comme en lien direct avec la pathologie, c’est-a-dire avec une imputabilite moyenne (directe mais non essentielle) ou forte (directe et essentielle). Une analyse des commentaires saisis pour chacun des dossiers a ete realisee. Resultats Entre 2011 et 2016, 244 de probleme de sante (PST) de cancers du larynx ont ete enregistres dans la base du rnv3p. Parmi eux, 147 ont ete conclus comme d’origine professionnelle dont 87 juges par le medecin expert avec une imputabilite moyenne ou forte. Cette population est exclusivement masculine, avec un âge median de 59 ans. Sur 97 expositions professionnelles retrouvees parmi ces 87 dossiers, 78 concernent l’amiante. Les secteurs d’activite les plus representes correspondent a ceux des travaux de construction specialises (14 dossiers) et de la metallurgie (7 dossiers). Pour 60 cas sur les 87 etudies, une declaration de maladie professionnelle a ete conseillee au patient. Discussion L’exposition a l’amiante est la situation professionnelle a risque la plus decrite dans les cas de cancer du larynx recenses au sein de la base de 2001 a 2016. Cela apporte un element supplementaire quant a l’implication de l’amiante dans la cancerogenicite pour le larynx. Une reflexion sur la mise en place d’un nouveau tableau de maladie professionnelle paraitrait legitime de ce point de vue.
Published: 2018

6. Cancers et expositions professionnelles : données du Réseau national de vigilance et de prévention des pathologies professionnelles (RNV3P) 2001–2016

Author: PARIS, Christophe, BALDI, Isabelle, BENSEFA-COLAS, Lynda, BLOC, Juliette, BONNETERRE, Vincent, BRUNEL, Serge, CLIN, Benedicte, DURAND-MOREAU, Quentin, GAIN, Muriel, HERIN, Fabrice, LARABI, Lynda, NISSE, Catherine, NOURRY, Nathalie, VANRULLEN, Isabelle, VONGMANY, Natalie, CHU-Hôpitaux de Rouen, CCPPE, Cancer environnement (EPICENE ), Bordeaux population health (BPH), Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Environnement et Prédiction de la Santé des Populations (TIMC-IMAG-EPSP), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre Hospitalier Universitaire [Grenoble] (CHU), Hôpital Côte de Nacre [CHU Caen], CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Hôpital Morvan - CHRU de Brest (CHU - BREST ), Service de Pneumologie et Pathologie Professionnelle, CHI Créteil-Faculté de Médecine, Epidémiologie et analyses en santé publique : risques, maladies chroniques et handicaps (LEASP), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de consultations de pathologies professionnelles [CHU Toulouse], CHU Toulouse [Toulouse], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), and Les Hôpitaux Universitaires de Strasbourg (HUS)
Subjects: 030506 rehabilitation, 03 medical and health sciences, Surveillance, 0302 clinical medicine, Expositions professionnelles, [SDV]Life Sciences [q-bio], Public Health, Environmental and Occupational Health, 0305 other medical science, 030210 environmental & occupational health, ComputingMilieux_MISCELLANEOUS, Cancer
Abstract: International audience
Published: 2018

7. Infertility in an adult cohort with primary ciliary dyskinesia: phenotype–gene association

Author: Isabelle Honoré, Bernard Maitre, André Coste, Mieke Boon, Jean-François Papon, Estelle Escudier, Harry Cuppens, Martine Jaspers, Rahma Mani, Natalie Lorent, L. Bassinet, Marie Legendre, Gert Jan Vanaken, Sophie Christin-Maitre, Serge Amselem, Service de génétique et embryologie médicales [CHU Trousseau], CHU Trousseau [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de Pneumologie [CHI Créteil], CHI Créteil, University Hospitals Leuven [Leuven], Service de pneumologie [CHU Cochin], Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d’ORL et de chirurgie cervico-faciale [CHU Le Kremlin-Bicêtre], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), IMRB - 'Biomechanics and Respiratory Apparatus' [Créteil] (U955 Inserm - UPEC), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service d'ORL [Créteil], Centre Hospitalier Intercommunal de Créteil (CHIC), Maladies génétiques d'expression pédiatrique [CHU Trousseau] (Inserm U933), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Trousseau [APHP], Service de Pneumologie et Pathologie Professionnelle, CHI Créteil-Faculté de Médecine, Service d'Endocrinologie, diabétologie et endocrinologie de la reproduction [CHU Saint-Antoine], CHU Saint-Antoine [AP-HP], and Couvet, Sandrine
Subjects: 0301 basic medicine, Pulmonary and Respiratory Medicine, Infertility, Adult, Male, [SDV]Life Sciences [q-bio], [SDV.GEN.GH] Life Sciences [q-bio]/Genetics/Human genetics, MESH: Infertility, Male, MESH: Phenotype, Andrology, Cohort Studies, 03 medical and health sciences, Genetic Heterogeneity, 0302 clinical medicine, otorhinolaryngologic diseases, Medicine, Humans, Gene, MESH: Cohort Studies, Infertility, Male, Primary ciliary dyskinesia, MESH: Middle Aged, MESH: Humans, MESH: Infertility, Female, business.industry, Genetic heterogeneity, MESH: Genetic Heterogeneity, MESH: Adult, Middle Aged, medicine.disease, Phenotype, Gene association, MESH: Male, [SDV] Life Sciences [q-bio], 030104 developmental biology, 030228 respiratory system, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, Cohort, Female, MESH: Ciliary Motility Disorders, business, Infertility, Female, MESH: Female, Cohort study, Ciliary Motility Disorders
Abstract: Infertility, observed in 75% of male and 61% of female PCD patients, is dependent on ultrastructural and gene defects http://ow.ly/P4K030fPnPp
Published: 2017

8. Threshold regression models adapted to case-control studies. Application to lung cancer induced by occupational exposure to asbestos

Author: Antoine Chambaz, Dominique Choudat, Catherine Huber, Jean-Claude Pairon, Mark van Der Laan, Modélisation aléatoire de Paris X (MODAL'X), Université Paris Nanterre (UPN), Institut Interuniversitaire de Médecine du Travail de Paris Ile-de-France, Service de pathologie professionnelle, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5), Mathématiques Appliquées Paris 5 (MAP5 - UMR 8145), Université Paris Descartes - Paris 5 (UPD5)-Institut National des Sciences Mathématiques et de leurs Interactions (INSMI)-Centre National de la Recherche Scientifique (CNRS), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Pneumologie et Pathologie Professionnelle, CHI Créteil, Institut Interuniversitaire de Médecine du Travail de Paris-Ile de France, Université Biomédicale des Saints-Pères, Division of Biostatistics [Berkeley], School of Public Health [Berkeley], University of California [Berkeley], University of California-University of California-University of California [Berkeley], University of California-University of California, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5), University of California [Berkeley] (UC Berkeley), University of California (UC)-University of California (UC)-University of California [Berkeley] (UC Berkeley), University of California (UC)-University of California (UC), Chambaz, Antoine, Modélisation aléatoire de Paris X ( MODAL'X ), Université Paris Nanterre ( UPN ), Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Descartes - Paris 5 ( UPD5 ), Mathématiques Appliquées à Paris 5 ( MAP5 - UMR 8145 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National des Sciences Mathématiques et de leurs Interactions-Centre National de la Recherche Scientifique ( CNRS ), Institut Mondor de Recherche Biomédicale ( IMRB ), Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IFR10, and Division of Biostatistics, School of Public Health
Subjects: [STAT.TH] Statistics [stat]/Statistics Theory [stat.TH], Threshold regression model, [MATH.MATH-ST]Mathematics [math]/Statistics [math.ST], Case-control study, Cross-validation, [ MATH.MATH-ST ] Mathematics [math]/Statistics [math.ST], [STAT.TH]Statistics [stat]/Statistics Theory [stat.TH], [ STAT.TH ] Statistics [stat]/Statistics Theory [stat.TH], [MATH.MATH-ST] Mathematics [math]/Statistics [math.ST]
Abstract: International audience; We rely on a recent French matched case-control study to investigate the effect of occupational exposure to asbestos on the occurrence of lung cancer. We build a large collection of threshold regression models, data-adaptively select a better model in it by multi-fold likelihood-based cross-validation, then fit the resulting better model by maximum likelihood. A necessary preliminary step to eliminate the bias due to the case-control sampling is made possible because the conditional distribution of being a case given the matching variable and the marginal distribution of the matching variable can be computed beforehand based on two studies independent from our dataset. The implications of the fitted model in terms of expected years of life free of lung cancer lost due to the occupational exposure to asbestos are discussed.
Published: 2014

9. Critical role of surface chemical modifications induced by length shortening on multi-walled carbon nanotubes-induced toxicity

Author: Julien Cambedouzou, Pascale Launois, Cyrill Bussy, Martine Mayne-L’Hermite, Sophie Lanone, Mathieu Pinault, Pascale Jégou, Marion Julie Landry, Jorge Boczkowski, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Laboratoire de Physique des Solides (LPS), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Francis PERRIN (LFP - URA 2453), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Service de Physique et de Chimie des Surfaces et Interfaces (SPCSI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Service de Pneumologie et Pathologie Professionnelle, CHI Créteil-Faculté de Médecine, Service de physiologie, explorations fonctionnelles [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), This work was supported by the Région Ile-de-France in the framework of C'nano IdF (NANOTUBTOX project), as well as for the MEG FEG instrument used, C'Nano-IdF is the nanoscience competence center of Paris Region, supported by CNRS, CEA, MESR and Région Ile-de-France., BMC, Ed., and Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)
Subjects: Materials science, Cell Survival, Surface Properties, Length, Health, Toxicology and Mutagenesis, lcsh:Industrial hygiene. Industrial welfare, Carbon nanotubes, Gene Expression, Nanotechnology, 02 engineering and technology, Carbon nanotube, Toxicology, Nanomaterials, Catalysis, law.invention, Cell Line, Stress (mechanics), 03 medical and health sciences, Mice, law, lcsh:RA1190-1270, Animals, Reactivity (chemistry), Viability assay, RNA, Messenger, Particle Size, 030304 developmental biology, lcsh:Toxicology. Poisons, Aerosols, 0303 health sciences, Nanotubes, Carbon, Research, Macrophages, General Medicine, 021001 nanoscience & nanotechnology, Surface chemistry, Oxidative Stress, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Biophysics, Nanomedicine, Cytokines, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Particle size, 0210 nano-technology, Oxidoreductases, lcsh:HD7260-7780.8
Abstract: Given the increasing use of carbon nanotubes (CNT) in composite materials and their possible expansion to new areas such as nanomedicine which will both lead to higher human exposure, a better understanding of their potential to cause adverse effects on human health is needed. Like other nanomaterials, the biological reactivity and toxicity of CNT were shown to depend on various physicochemical characteristics, and length has been suggested to play a critical role. We therefore designed a comprehensive study that aimed at comparing the effects on murine macrophages of two samples of multi-walled CNT (MWCNT) specifically synthesized following a similar production process (aerosol-assisted CVD), and used a soft ultrasonic treatment in water to modify the length of one of them. We showed that modification of the length of MWCNT leads, unavoidably, to accompanying structural (i.e. defects) and chemical (i.e. oxidation) modifications that affect both surface and residual catalyst iron nanoparticle content of CNT. The biological response of murine macrophages to the two different MWCNT samples was evaluated in terms of cell viability, pro-inflammatory cytokines secretion and oxidative stress. We showed that structural defects and oxidation both induced by the length reduction process are at least as responsible as the length reduction itself for the enhanced pro-inflammatory and pro-oxidative response observed with short (oxidized) compared to long (pristine) MWCNT. In conclusion, our results stress that surface properties should be considered, alongside the length, as essential parameters in CNT-induced inflammation, especially when dealing with a safe design of CNT, for application in nanomedicine for example.
Published: 2012

10. Pulmonary carcinoid tumors and asbestos exposure

Author: Bruno Housset, Isabelle Monnet, Claire Danel, Françoise Galateau-Sallé, Françoise Le Pimpec-Barthes, Issam Abd Al Samad, Jean-Claude Pairon, Marc Riquet, Chantal Azpitarte, Bénédicte Clin, Pascal Andujar, Karinne Legrand-Cattan, M. A. Billon-Galland, Mireille Matrat, Service de Santé au Travail et Pathologie Professionnellel [CHU Caen], Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Pneumologie et Pathologie Professionnelle, CHI Créteil, Service d'anatomie pathologique, Service de santé au travail, SNCF, Service de chirurgie thoracique, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5), Laboratoire d'Etude des Particules Inhalées (LEPI), Ville de Paris, Service d'Anatomie et cytologie pathologique, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Laboratoire d'Anatomie Pathologique [CHU Caen], Pôle Santé Travail, Guellaen, Georges, Service de santé au travail et pathologie professionnelle, CHU Caen, Institut Mondor de Recherche Biomédicale ( IMRB ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 ), Assistance publique - Hôpitaux de Paris (AP-HP)-Hôpital Européen Georges Pompidou [APHP] ( HEGP ) -Université Paris Descartes - Paris 5 ( UPD5 ), Laboratoire d'Etude des Particules Inhalées ( LEPI ), and Assistance publique - Hôpitaux de Paris (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris]-Université Paris Diderot - Paris 7 ( UPD7 )
Subjects: Adult, Male, medicine.medical_specialty, Pathology, Paris, Lung Neoplasms, Carcinoid tumors, Carcinoid Tumor, medicine.disease_cause, Gastroenterology, Asbestos, Article, Age and gender, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, Surveys and Questionnaires, medicine, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Lung cancer, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Aged, Retrospective Studies, Lung, business.industry, Public Health, Environmental and Occupational Health, Case-control study, Retrospective cohort study, General Medicine, pulmonary carcinoid tumor, occupational exposure, Middle Aged, medicine.disease, asbestos, 3. Good health, Occupational Diseases, lung cancer, medicine.anatomical_structure, 030228 respiratory system, 030220 oncology & carcinogenesis, Case-Control Studies, Female, business, Lung tissue
Abstract: International audience; OBJECTIVES: The hypothesis that asbestos exposure may have more specific associations with particular histological types of lung cancer remains controversial. The aim of this study was to analyze the relationships between asbestos exposure and pulmonary carcinoid tumors. METHODS: A retrospective case-control study was conducted in 28 cases undergoing surgery for pulmonary carcinoid tumors and aged >40 years and in 56 controls with lung cancer of a different histological type, matched for gender and age, from 1994 to 1999, recruited in two hospitals in the region of Paris. Asbestos exposure was assessed via expertise of a standardized occupational questionnaire and mineralogical analysis of lung tissue, with quantification of asbestos bodies (AB). RESULTS: Definite asbestos exposure was identified in 25% of cases and 14% of controls (ns). Cumulative asbestos exposure was significantly higher in cases than in controls (P < 0.05), and results of the quantification of AB tended to be higher in cases than in controls (24 and 9% had >1000 AB per gram dry lung tissue, respectively, P = 0.09). Mean cumulative smoking was lower in cases than in controls (P < 0.05). CONCLUSIONS: This study argues in favor of a relationship between asbestos exposure and certain pulmonary carcinoid tumors.
Published: 2012

11. Intratracheally administered titanium dioxide or carbon black nanoparticles do not aggravate elastase-induced pulmonary emphysema in rats

Author: Maylis Dagouassat, Jeanne Tran Van Nhieu, Lucie Armand, Ghislaine Lacroix, Angélique Simon-Deckers, Agnès Roulet, Françoise Rogerieux, Esther Belade, Jorge Boczkowski, Sophie Lanone, Jean-Claude Pairon, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut National de l'Environnement Industriel et des Risques (INERIS), Service de Pathologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor, Pneumologie et Pathologie Professionnelle, CHI Créteil, Service de Physiologie Explorations Fonctionnelles, This work was supported by the 'Agence Nationale de la Recherche France' (Nanotox project, 05 9 79-05 SET 024-01), the 'Société de Pneumologie de Langue Française' for a master's grant for Agnès Roulet, the 'ABIES' PhD program for a PhD grant for Lucie Armand, and the 'Agence nationale de sécurité sanitaire de l'Alimentation, de l'Environnement et du Travail' (ANSES) for a PhD grant and funding for Esther Belade, BMC, Ed., and Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10
Subjects: Male, Pathology, Interleukin-1beta, 010501 environmental sciences, 01 natural sciences, Bronchoalveolar Lavage, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Rats, Sprague-Dawley, Bovine serum albumin, Pancreatic elastase, Titanium, 0303 health sciences, medicine.diagnostic_test, biology, Pancreatic Elastase, Elastase, respiratory system, medicine.anatomical_structure, Particles, Pulmonary Emphysema, Toxicity, Models, Animal, medicine.symptom, Research Article, Pulmonary and Respiratory Medicine, medicine.medical_specialty, Inflammation, 03 medical and health sciences, Soot, Matrix Metalloproteinase 12, medicine, Intubation, Intratracheal, Animals, COPD, 030304 developmental biology, 0105 earth and related environmental sciences, lcsh:RC705-779, Lung, business.industry, Monocyte, technology, industry, and agriculture, Pneumonia, lcsh:Diseases of the respiratory system, Rats, Bronchoalveolar lavage, biology.protein, Nanoparticles, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Occupational medicine, business, Heme Oxygenase-1
Abstract: Background Titanium dioxide (TiO2) and carbon black (CB) nanoparticles (NPs) have biological effects that could aggravate pulmonary emphysema. The aim of this study was to evaluate whether pulmonary administration of TiO2 or CB NPs in rats could induce and/or aggravate elastase-induced emphysema, and to investigate the underlying molecular mechanisms. Methods On day 1, Sprague-Dawley rats were intratracheally instilled with 25 U kg−1 pancreatic porcine elastase or saline. On day 7, they received an intratracheal instillation of TiO2 or CB (at 100 and 500 μg) dispersed in bovine serum albumin or bovine serum albumin alone. Animals were sacrificed at days 8 or 21, and bronchoalveolar lavage (BAL) cellularity, histological analysis of inflammation and emphysema, and lung mRNA expression of heme oxygenase-1 (HO-1), interleukin-1β (IL-1β), macrophage inflammatory protein-2, monocyte chemotactic protein-1, and matrix metalloprotease (MMP)-1, and -12 were measured. In addition, pulmonary MMP-12 expression was also analyzed at the protein level by immunohistochemistry. Results TiO2 NPs per se did not modify the parameters investigated, but CB NPs increased perivascular/peribronchial infiltration, and macrophage MMP-12 expression, without inducing emphysema. Elastase administration increased BAL cellularity, histological inflammation, HO-1, IL-1β and macrophage MMP-12 expression and induced emphysema. Exposure to TiO2 NPs did not modify pulmonary responses to elastase, but exposure to CB NPs aggravated elastase-induced histological inflammation without aggravating emphysema. Conclusions TiO2 and CB NPs did not aggravate elastase-induced emphysema. However, CB NPs induced histological inflammation and MMP-12 mRNA and protein expression in macrophages.
Published: 2012

12. INTERACTION OF MATRIX METALLOPROTEINASES WITH PULMONARY POLLUTANTS

Author: Dagouassat, Maylis, Lanone, Sophie, Boczkowski, Jorge, IMRB - GEIC2O/'Genetic and Environmental Interactions in COPD, Cystic fibrosis and Other (rare) respiratory diseases' [Créteil] (U955 Inserm - UPEC), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de Pneumologie et de Pathologie Professionnelle [CHI Créteil], CHI Créteil, Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Pneumologie et Pathologie Professionnelle, Service de physiologie, explorations fonctionnelles [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Maylis Dagouassat was supported by a grant of DIM SENT (Région Ile-de-France) and by the CARESEN foundation, Jorge Boczkowski by INSERM and Assistance Publique- Hôpitaux de Paris (Contrat Hospitalier de Recherche Translationnelle), and Sophie Lanone by INSERM and Centre Hospitalier Intercommunal de Créteil (Contrat Hospitalier de Recherche Translationnelle)., Lanone, Sophie, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, and Guellaen, Georges
Subjects: Lung Diseases, diesel, Air Pollutants, matrix metalloproteinases (MMP), [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, lung and systemic disease, particulate exposure, Humans, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Lung, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Matrix Metalloproteinases
Abstract: International audience; An air pollutant consists of any atmospheric substance that may harm humans, animals, vegetation or material. Various air pollutants have been reported, differing in their physicochemical characteristics. They can be grouped into four categories: gaseous pollutants (e.g. ozone, sulfur dioxide, oxides of nitrogen, carbon monoxide and volatile organic compounds), persistent organic pollutants, heavy metals (e.g. cadmium, lead and mercury) and particulate matter (coarse, fine and ultrafine). These pollutants can reach the respiratory system, eliciting pulmonary and/or systemic effects. These effects include inflammation, tissue remodelling and carcinogenesis: all phenomena where matrix metalloproteases (MMPs) play critical roles, given their broad effects on matrix remodelling and modulation of inflammation and cell signalling. Moreover, since expression and activity of MMPs can be induced by such stimuli, the hypothesis has been raised that MMPs could be involved in the health effects of pollutants. Until now, the implication of MMPs in these effects has been studied only for some pollutants and for a restricted selection of MMPs (mainly MMP-1, -2, -9 and -12), while evidence for a link between MMP induction/activation and health effects remains scarce. A larger number of studies is, therefore, needed in order to better understand the implication of MMPs in health effects associated with air pollution.
Published: 2012

13. A comparative transmission electron microscopy study of titanium dioxide and carbon black nanoparticles uptake in human lung epithelial and fibroblast cell lines

Author: M. A. Billon-Galland, Jorge Boczkowski, Esther Belade, Armelle Baeza-Squiban, Jocelyne Fleury-Feith, Laurent Martinon, Jean-Claude Pairon, Lucie Armand, Sophie Lanone, L. Kheuang, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Hôpital Henri Mondor, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Laboratoire d'Etude des Particules Inhalées (LEPI), Ville de Paris, CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire de Cytophysiologie et Toxicologie Cellulaire (LCTC), Université Paris Diderot - Paris 7 (UPD7), Service de pneumologie et pathologie professionnelle, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor, This work was supported by the 'Agence Nationale de la Recherche France' (Nanotox project, ANR N° 05979-5 SET 024-01), by the Department of Paris, by the 'Agence nationale de sécurité sanitaire de l'Alimentation, de l'Environnement et du Travail' for a PhD grant andfunding of Esther Belade, and by the 'ABIES' PhD program for a PhD grant of Lucie Armand., Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and Guellaen, Georges
Subjects: Cell type, Nanotechnology, 02 engineering and technology, Toxicology, Cell Line, 03 medical and health sciences, chemistry.chemical_compound, Microscopy, Electron, Transmission, Soot, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, medicine, MESH: Soot, Humans, MESH: Lung, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: Particle Size, Particle Size, Coloring Agents, Cytotoxicity, Fibroblast, Lung, 030304 developmental biology, Titanium, 0303 health sciences, MESH: Humans, Vesicle, Epithelial Cells, General Medicine, Fibroblasts, 021001 nanoscience & nanotechnology, MESH: Cell Line, Cytosol, medicine.anatomical_structure, chemistry, MESH: Titanium, Cell culture, MESH: Epithelial Cells, MESH: Fibroblasts, Titanium dioxide, Biophysics, MESH: Microscopy, Electron, Transmission, Nanoparticles, 0210 nano-technology, Intracellular, MESH: Coloring Agents, MESH: Nanoparticles
Abstract: International audience; Several studies suggest that the biological responses induced by manufactured nanoparticles (MNPs) may be linked to their accumulation within cells. However, MNP internalisation has not yet been sufficiently characterised. Therefore, the aim of this study was to compare the intracellular uptake of three different MNPs: two made of carbon black (CB) and one made of titanium dioxide (TiO(2)), in 16HBE bronchial epithelial cells and MRC5 fibroblasts. Transmission electron microscopy was used to evaluate the intracellular accumulation. Different parameters were analysed following a time and dose-relationship: localisation of MNPs in cells, percentage of cells having accumulated MNPs, number of aggregated MNPs in cells, and the size of MNP aggregates in cells. The results showed that MNPs were widely and rapidly accumulated in 16HBE cells and MRC5 fibroblasts. Moreover, MNPs accumulated chiefly as aggregates in cytosolic vesicles and were absent from the mitochondria or nuclei. CB and TiO(2) MNPs had similar accumulation patterns. However, TiO(2) aggregates had a higher size than CB aggregates. Intracellular MNP accumulation was dissociated from cytotoxicity. These results suggest that cellular uptake of MNPs is a common phenomenon occurring in various cell types.
Published: 2012

14. Differential antibacterial activity against Pseudomonas aeruginosa by carbon monoxide-releasing molecules

Author: Mathieu, Desmard, Roberta, Foresti, Didier, Morin, Maylis, Dagouassat, Maylis, Dagoussat, Alain, Berdeaux, Erick, Denamur, Sian H, Crook, Brian E, Mann, David, Scapens, Philippe, Montravers, Jorge, Boczkowski, Roberto, Motterlini, Département anesthésie-réanimation chirurgicale, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Department of Drug Discovery and Development, Italian Institute of Technology (IIT), Ecologie et Evolution des Microorganismes (EEM), Université Paris Diderot - Paris 7 (UPD7)-Université Paris 13 (UP13)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Chemistry [Sheffield], University of Sheffield [Sheffield], Service de pneumologie et pathologie professionnelle, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor, and Guellaen, Georges
Subjects: Physiology, Dimer, Clinical Biochemistry, 01 natural sciences, Biochemistry, Oxygen, chemistry.chemical_compound, Mice, MESH: Animals, Carbon monoxide, Cells, Cultured, General Environmental Science, chemistry.chemical_classification, 0303 health sciences, MESH: Microbial Sensitivity Tests, MESH: Reactive Oxygen Species, Ruthenium, Anti-Bacterial Agents, Pseudomonas aeruginosa, MESH: Pseudomonas aeruginosa, Thiol, Antibacterial activity, Infection, MESH: Carbon Monoxide, MESH: Oxygen, MESH: Cells, Cultured, Free Radicals, Stereochemistry, chemistry.chemical_element, Microbial Sensitivity Tests, 010402 general chemistry, 03 medical and health sciences, MESH: Acetylcysteine, MESH: Anti-Bacterial Agents, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, MESH: Mice, 030304 developmental biology, Pharmacology, Reactive oxygen species, MESH: Humans, Cell Biology, Carbon monoxide-releasing molecules, 0104 chemical sciences, Acetylcysteine, chemistry, General Earth and Planetary Sciences, Reactive Oxygen Species
Abstract: International audience; AIMS: Carbon monoxide (CO) delivered in a controlled manner to cells and organisms mediates a variety of pharmacological effects to the extent that CO-releasing molecules (CO-RMs) are being developed for therapeutic purposes. Recently, ruthenium-based CO-RMs have been shown to posses important bactericidal activity. Here we assessed the effect of fast CO releasers containing ruthenium (Ru(CO)(3)Cl(glycinate) [CORM-3] and tricarbonyldichlororuthenium(II) dimer [CORM-2]) and a novel slow manganese-based CO releaser ([Me(4)N][Mn(CO)(4)(thioacetate)(2)] [CORM-371]) on O(2) consumption and growth of Pseudomonas aeruginosa (PAO1). We then compared these effects with the action elicited by sodium boranocarbonate (CORM-A1), which lacks a transition metal but liberates CO with a rate similar to CORM-371. RESULTS: CORM-2, CORM-3, and, to a lesser extent, CORM-371 exerted a significant bactericidal effect and decreased O(2) consumption in PAO1 in vitro. The effect appeared to be independent of reactive oxygen species production, but in the case of metal-containing compounds it was prevented by the thiol donor N-acetylcysteine. In contrast, CORM-A1 was bacteriostatic rather than bactericidal in vitro eliciting only a moderate and transient decrease in O(2) consumption. INNOVATION: None of the tested CO-RMs was toxic to murine macrophages or human fibroblasts at the concentration impairing PA01 growth but only ruthenium-containing CO-RMs showed potential therapeutic properties by increasing the survival of mice infected with PA01. CONCLUSION: CO carriers inhibit bacterial growth and O(2) consumption in vitro, but transition metal carbonyls appear more powerful than compounds spontaneously liberating CO. The nature of the metal in CO-RMs also modulates the anti-bacterial effect, with ruthenium-based CO-RMs being efficacious both in vitro and in vivo.
Published: 2012

15. Pulmonary nodules detected by thoracic computed tomography scan after exposure to asbestos: diagnostic significance

Author: Clin, Bénédicte, Luc, Amandine, Morlais, Fabrice, Paris, Chrisophe, Ameille, Jacques, Brochard, Patrick, De Girolamo, Julien, Gislard, Antoine, Laurent, François, Letourneux, Marc, Schorle, Evelyne, Launoy, Guy, Pairon, Jean-Claude, Cancers et Populations : Facteurs de Risque, Depistage, Pratiques Diagnostiques et Therapeutiques, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Santé au Travail et Pathologie Professionnellel [CHU Caen], Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Nutrition-Génétique et Exposition aux Risques Environnementaux (NGERE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Service de la Santé au Travail [Poincaré], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Raymond Poincaré [AP-HP], Service de médecine du travail (LSTE), CHU Bordeaux [Bordeaux], Service de santé au travail et pathologie professionnelle [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Nutrition et cerveau (U1213, U855), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Echelon Régional du service Médical (ERSM) Rhône-Alpes, Assurance Maladie, Pneumologie et Pathologie Professionnelle, CHI Créteil, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Grant sponsor: French National Health Insurance (Occupational Risk Prevention Department), French Ministry of Labour and Social Relations, AFSSET grant EST 2006/1/43, AFSSET grant 07-CRD-51, and National Network of Asbestos Post-Exposure Survey (APEXS)
Subjects: lung cancer, screening, CT Scan, pulmonary nodule, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, asbestos
Abstract: International audience; OBJECTIVE: To analyse the relationship between pulmonary nodules detected by radiologists using computed tomography and cumulative exposure to asbestos or asbestos-related pleuro-pulmonary diseases in 5662 asbestos-exposed subjects, and the relationship between pulmonary nodules and thoracic cancer, to determine whether a specific surveillance strategy based on cumulative asbestos exposure should be adopted. DESIGN: Standardised incidence and mortality ratios (SIR) for lung cancer and pleural mesothelioma were calculated in patients with and without mention of pulmonary nodules and compared using comparative morbidity figures. RESULTS: A significant excess incidence of primary lung cancer and pleural mesothelioma was observed among subjects presenting with pulmonary nodule(s) (SIR respectively 1.95, 95%CI 1.22-2.95, and 11.88, 95%CI 3.20-30.41). However, there was no significant relationship between pulmonary nodules mentioned by radiologists and cumulative asbestos exposure or between pulmonary nodules and the presence of asbestos-related benign diseases. CONCLUSIONS: This study confirms the expected excess prevalence of lung cancer in subjects presenting with pulmonary nodules according to the radiologist's report, and shows the absence of relationship between the presence of nodules and level of cumulative asbestos exposure. Our study therefore offers no argument in favour of specific surveillance modalities based on estimated cumulative asbestos exposure.
Published: 2011

16. Pulmonary nodules detected by thoracic computed tomography scan after exposure to asbestos: diagnostic significance

Author: B, Clin, A, Luc, F, Morlais, C, Paris, J, Ameille, P, Brochard, J, De Girolamo, A, Gislard, F, Laurent, M, Letourneux, E, Schorle, G, Launoy, J-C, Pairon, A, Stoufflet, Cancers et Populations : Facteurs de Risque, Depistage, Pratiques Diagnostiques et Therapeutiques, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Santé au Travail et Pathologie Professionnellel [CHU Caen], Normandie Université (NU)-Normandie Université (NU)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Tumorothèque de Caen Basse-Normandie (TCBN), Nutrition-Génétique et Exposition aux Risques Environnementaux (NGERE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Service de la Santé au Travail [Poincaré], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Raymond Poincaré [AP-HP], Service de médecine du travail (LSTE), CHU Bordeaux [Bordeaux], Service de santé au travail et pathologie professionnelle [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Nutrition et cerveau (U1213, U855), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Echelon Régional du service Médical (ERSM) Rhône-Alpes, Assurance Maladie, Pneumologie et Pathologie Professionnelle, CHI Créteil, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Grant sponsor: French National Health Insurance (Occupational Risk Prevention Department), French Ministry of Labour and Social Relations, AFSSET grant EST 2006/1/43, AFSSET grant 07-CRD-51, and National Network of Asbestos Post-Exposure Survey (APEXS)
Subjects: Pulmonary and Respiratory Medicine, Male, Mesothelioma, medicine.medical_specialty, Lung Neoplasms, Pleural Neoplasms, Cumulative Exposure, medicine.disease_cause, Asbestos, Article, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Occupational Exposure, medicine, CT Scan, Prevalence, Humans, Mass Screening, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Pleural Neoplasm, Lung cancer, Mass screening, Aged, Solitary pulmonary nodule, Multiple Pulmonary Nodules, business.industry, screening, Incidence, Solitary Pulmonary Nodule, pulmonary nodule, Middle Aged, medicine.disease, 3. Good health, lung cancer, Infectious Diseases, 030220 oncology & carcinogenesis, Population Surveillance, Carcinogens, Female, Radiology, Nuclear medicine, business, Tomography, X-Ray Computed
Abstract: International audience; OBJECTIVE: To analyse the relationship between pulmonary nodules detected by radiologists using computed tomography and cumulative exposure to asbestos or asbestos-related pleuro-pulmonary diseases in 5662 asbestos-exposed subjects, and the relationship between pulmonary nodules and thoracic cancer, to determine whether a specific surveillance strategy based on cumulative asbestos exposure should be adopted. DESIGN: Standardised incidence and mortality ratios (SIR) for lung cancer and pleural mesothelioma were calculated in patients with and without mention of pulmonary nodules and compared using comparative morbidity figures. RESULTS: A significant excess incidence of primary lung cancer and pleural mesothelioma was observed among subjects presenting with pulmonary nodule(s) (SIR respectively 1.95, 95%CI 1.22-2.95, and 11.88, 95%CI 3.20-30.41). However, there was no significant relationship between pulmonary nodules mentioned by radiologists and cumulative asbestos exposure or between pulmonary nodules and the presence of asbestos-related benign diseases. CONCLUSIONS: This study confirms the expected excess prevalence of lung cancer in subjects presenting with pulmonary nodules according to the radiologist's report, and shows the absence of relationship between the presence of nodules and level of cumulative asbestos exposure. Our study therefore offers no argument in favour of specific surveillance modalities based on estimated cumulative asbestos exposure.
Published: 2011

17. Intoxication au mercure

Author: Bensefa-Colas, Lynda, Andujar, Pascal, Descatha, Alexis, Département de Médecine du Travail, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut Interuniversitaire de Médecine du Travail de Paris Ile de France, Service de pneumologie et pathologie professionnelle, CHI Créteil, Institut Mondor de recherche biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Santé publique et épidémiologie des déterminants professionnels et sociaux de la santé, Epidémiologie, sciences sociales, santé publique (IFR 69), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-École des hautes études en sciences sociales (EHESS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-École des hautes études en sciences sociales (EHESS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Unité de pathologie professionnelle, environnementale et d'insertion, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Raymond Poincaré [AP-HP], and Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-École des hautes études en sciences sociales (EHESS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-École des hautes études en sciences sociales (EHESS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: methylmercure, toxicité, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, mercure
Abstract: International audience; Mercury is a widespread heavy metal with potential severe impacts on human health. Exposure conditions to mercury and profile of toxicity among humans depend on the chemical forms of the mercury: elemental or metallic mercury, inorganic or organic mercury compounds. This article aims to reviewing and synthesizing the main knowledge of the mercury toxicity and its organic compounds that clinicians should know. Acute inhalation of metallic or inorganic mercury vapours mainly induces pulmonary diseases, whereas chronic inhalation rather induces neurological or renal disorders (encephalopathy and interstitial or glomerular nephritis). Methylmercury poisonings from intoxicated food occurred among some populations resulting in neurological disorders and developmental troubles for children exposed in utero. Treatment using chelating agents is recommended in case of symptomatic acute mercury intoxication; sometimes it improves the clinical effects of chronic mercury poisoning. Although it is currently rare to encounter situations of severe intoxication, efforts remain necessary to decrease the mercury concentration in the environment and to reduce risk on human health due to low level exposure (dental amalgam, fish contamination by organic mercury compounds...). In case of occupational exposure to mercury and its compounds, some disorders could be compensated in France. Clinicians should work with toxicologists for the diagnosis and treatment of mercury intoxication.
Published: 2011

18. Syntenic relationships between genomic profiles of fiber-induced murine and human malignant mesothelioma

Author: Jean, Didier, Thomas, Emilie, Manié, Elodie, Renier, Annie, De Reynies, Aurélien, Lecomte, Céline, Andujar, Pascal, Fleury-Feith, Jocelyne, Galateau-Sallé, Françoise, Giovannini, Marco, Zucman-Rossi, Jessica, Stern, Marc-Henri, Jaurand, Marie-Claude, Génomique Fonctionnelle des Tumeurs Solides (U1162), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), Ligue Nationale Contre le Cancer - Paris, Ligue Nationnale Contre le Cancer, Unité de génétique et biologie des cancers (U830), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Ecotoxicologie - Milieux Aquatiques (LEMA), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Pneumologie et Pathologie Professionnelle, CHI Créteil, CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Cancers et Populations : Facteurs de Risque, Depistage, Pratiques Diagnostiques et Therapeutiques, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'anatomie pathologique, House Ear Institute, Center for Neural Tumor Research, Agence Nationale de la Recherche 05 9 31/ANR 05, Ligue Nationale Contre le Cancer: CIT1 and CIT2 programs (CIT: Carte d'Identité des Tumeurs), Ministère de l'Emploi, de la Solidarité n°1D004C, de l'Environnement n°AC008B, Ligue Nationale Contre le Cancer (comité de l'Oise, France), and Agence Nationale de la Recherche 05 9 31/ANR 05 and Agence Française de Sécurité Sanitaire de l'Environnement et du Travail RD-2004-015.
Subjects: Mesothelioma, MESH: Xenograft Model Antitumor Assays, MESH: Mutation, [SDV.CAN]Life Sciences [q-bio]/Cancer, Mouse models, MESH: Chromosomes, Human, MESH: Pleural Neoplasms, MESH: Mineral Fibers, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], array CGH, MESH: Genome, MESH: Animals, MESH: Mice, MESH: Genetic Association Studies, MESH: Aged, MESH: Humans, MESH: Middle Aged, MESH: Mesothelioma, MESH: Genomic Instability, MESH: Genomics, MESH: Alleles, Mineral fibers, MESH: Synteny, MESH: Male, MESH: Comparative Genomic Hybridization, MESH: Microarray Analysis, MESH: Models, Animal, MESH: Gene Deletion, MESH: Asbestos, MESH: Female
Abstract: International audience; Malignant mesothelioma (MM) is an aggressive tumor with a poor prognosis mainly linked to past asbestos exposure. Murine models of MM based on fiber exposure have been developed to elucidate the mechanism of mesothelioma formation. Genomic alterations in murine MM have now been partially characterized. To gain insight into the pathophysiology of mesothelioma, 16 murine and 35 human mesotheliomas were characterized by array-comparative genomic hybridization and were screened for common genomic alterations. Alteration of the 9p21 human region, often by biallelic deletion, was the most frequent alteration in both species, in agreement with the CDKN2A/CDKN2B locus deletion in human disease and murine models. Other shared aberrations were losses of 1p36.3-p35 and 13q14-q33 and gains of 5p15.3-p13 regions. However, some differences were noted, such as absence of recurrent alterations in mouse regions corresponding to human chromosome 22. Comparison between altered recurrent regions in asbestos-exposed and non-asbestos-exposed patients showed a significant difference in the 14q11.2-q21 region, which was also lost in fiber-induced murine mesothelioma. A correlation was also demonstrated between genomic instability and tumorigenicity of human mesothelioma xenografts in nude mice. Overall, these data show similarities between murine and human disease, and contribute to the understanding of the influence of fibers in the pathogenesis of mesothelioma and validation of the murine model for preclinical testing.
Published: 2011

19. Syntenic relationships between genomic profiles of fiber-induced murine and human malignant mesothelioma

Author: Françoise Galateau-Sallé, Elodie Manié, Céline Lecomte, Jessica Zucman-Rossi, Aurélien de Reyniès, Pascal Andujar, Jocelyne Fleury-Feith, Marc-Henri Stern, Annie Renier, Marie-Claude Jaurand, Didier Jean, Emilie Thomas, Marco Giovannini, Genomique Fonctionnelle des Tumeurs Solides, Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ) -IFR105-Université Paris Diderot - Paris 7 ( UPD7 ), Université Paris Descartes - Paris 5 ( UPD5 ), Ligue Nationale Contre le Cancer - Paris, Ligue Nationnale Contre le Cancer, Unité de génétique et biologie des cancers ( U830 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut Curie-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire d'Ecotoxicologie - Milieux Aquatiques ( LEMA ), Université Le Havre Normandie ( ULH ), Normandie Université ( NU ) -Normandie Université ( NU ), Institut Mondor de Recherche Biomédicale ( IMRB ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 ), Pneumologie et Pathologie Professionnelle, CHI Créteil, Laboratoire d'histologie et de biologie tumorale, Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Tenon [APHP], Cancers et Populations : Facteurs de Risque, Depistage, Pratiques Diagnostiques et Therapeutiques, Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Service d'anatomie pathologique, House Ear Institute, Center for Neural Tumor Research, Ligue Nationale Contre le Cancer: CIT1 and CIT2 programs (CIT: Carte d'Identité des Tumeurs), Ministère de l'Emploi, de la Solidarité n°1D004C, de l'Environnement n°AC008B, Ligue Nationale Contre le Cancer (comité de l'Oise, France), Agence Nationale de la Recherche 05 9 31/ANR 05 and Agence Française de Sécurité Sanitaire de l'Environnement et du Travail RD-2004-015., Agence Nationale de la Recherche 05 9 31/ANR 05,Agence Nationale de la Recherche 05 9 31/ANR 05, Génomique Fonctionnelle des Tumeurs Solides (U1162), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Descartes - Paris 5 (UPD5), Ligue Nationale Contre le Cancer (LNCC), Unité de génétique et biologie des cancers (U830), Université Paris Descartes - Paris 5 (UPD5)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Ecotoxicologie - Milieux Aquatiques (LEMA), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), CHU Tenon [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Agence Nationale de la Recherche 05 9 31/ANR 05
Subjects: Genome instability, Male, Mesothelioma, MESH : Mesothelioma, MESH : Pleural Neoplasms, MESH : Aged, MESH : Gene Deletion, Mouse models, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, Pathogenesis, Mice, 0302 clinical medicine, CDKN2A, CDKN2B, Chromosomes, Human, array CGH, MESH : Mineral Fibers, MESH: Animals, MESH : Female, MESH : Comparative Genomic Hybridization, MESH: Genetic Association Studies, MESH: Aged, 0303 health sciences, Comparative Genomic Hybridization, Genome, MESH: Middle Aged, MESH : Asbestos, MESH: Genomic Instability, MESH: Genomics, Mineral fibers, Regular Article, MESH: Synteny, Genomics, Middle Aged, 3. Good health, MESH : Microarray Analysis, MESH: Models, Animal, [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], 030220 oncology & carcinogenesis, MESH: Asbestos, Female, MESH : Mutation, MESH: Xenograft Model Antitumor Assays, MESH: Mutation, Pleural Neoplasms, MESH : Male, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, MESH : Xenograft Model Antitumor Assays, MESH: Chromosomes, Human, Synteny, Genomic Instability, MESH: Pleural Neoplasms, Pathology and Forensic Medicine, MESH: Mineral Fibers, 03 medical and health sciences, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], MESH : Mice, medicine, Animals, Humans, MESH: Genome, MESH : Middle Aged, Allele, MESH: Mice, Alleles, Genetic Association Studies, 030304 developmental biology, Aged, MESH: Humans, MESH: Mesothelioma, MESH: Alleles, MESH : Genomics, MESH : Humans, MESH : Synteny, Asbestos, MESH : Genetic Association Studies, medicine.disease, Molecular biology, Xenograft Model Antitumor Assays, MESH: Male, MESH : Models, Animal, MESH : Chromosomes, Human, MESH: Comparative Genomic Hybridization, MESH: Microarray Analysis, MESH: Gene Deletion, Mutation, MESH : Animals, MESH : Genome, MESH : Alleles, Chromosome 22, MESH: Female, MESH : Genomic Instability, Gene Deletion, Comparative genomic hybridization
Abstract: International audience; Malignant mesothelioma (MM) is an aggressive tumor with a poor prognosis mainly linked to past asbestos exposure. Murine models of MM based on fiber exposure have been developed to elucidate the mechanism of mesothelioma formation. Genomic alterations in murine MM have now been partially characterized. To gain insight into the pathophysiology of mesothelioma, 16 murine and 35 human mesotheliomas were characterized by array-comparative genomic hybridization and were screened for common genomic alterations. Alteration of the 9p21 human region, often by biallelic deletion, was the most frequent alteration in both species, in agreement with the CDKN2A/CDKN2B locus deletion in human disease and murine models. Other shared aberrations were losses of 1p36.3-p35 and 13q14-q33 and gains of 5p15.3-p13 regions. However, some differences were noted, such as absence of recurrent alterations in mouse regions corresponding to human chromosome 22. Comparison between altered recurrent regions in asbestos-exposed and non-asbestos-exposed patients showed a significant difference in the 14q11.2-q21 region, which was also lost in fiber-induced murine mesothelioma. A correlation was also demonstrated between genomic instability and tumorigenicity of human mesothelioma xenografts in nude mice. Overall, these data show similarities between murine and human disease, and contribute to the understanding of the influence of fibers in the pathogenesis of mesothelioma and validation of the murine model for preclinical testing.
Published: 2011

20. NOX4/NADPH oxidase expression is increased in pulmonary fibroblasts from patients with idiopathic pulmonary fibrosis and mediates TGFbeta1-induced fibroblast differentiation into myofibroblasts

Author: Amara, Nadia, Goven, Delphine, Prost, Fabienne, Muloway, Rachel, Crestani, Bruno, Boczkowski, Jorge, Physiopathologie et Epidemiologie de l'Insuffisance Respiratoire, Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de pneumologie A, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP - Hôpital Bichat - Claude Bernard [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Diderot - Paris 7 (UPD7), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de physiologie, explorations fonctionnelles [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de pneumologie et pathologie professionnelle, CHI Créteil, Nadia Amara was supported by Chancellerie des Universités de Paris (legs Poix), and Jorge Boczkowski by INSERM and Assistance Publique-Hôpitaux de Paris (Contrat d'Interface). This work was supported by the European Commission (FP 7, European IPF Network) and by the Agence Nationale de la Recherche (ANR Physio 2006, FIBROPNEUMO)., ANR-06-PHYS-0011,FIBROPNEUMO,Fibroblastes et Fibrogénèse pulmonaire(2006), and European Project: 202224,EC:FP7:HEALTH,FP7-HEALTH-2007-A,EURIPFNET(2008)
Subjects: MESH: Aged, MESH: Cell Differentiation, MESH: Middle Aged, MESH: Humans, MESH: Gene Expression Regulation, Enzymologic, MESH: Idiopathic Pulmonary Fibrosis, MESH: Platelet-Derived Growth Factor, MESH: Reactive Oxygen Species, MESH: Adult, MESH: Male, MESH: Transforming Growth Factor beta1, MESH: Fibroblasts, MESH: Reverse Transcriptase Polymerase Chain Reaction, cardiovascular system, MESH: Up-Regulation, MESH: Smad3 Protein, MESH: Lung, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, MESH: NADPH Oxidase, MESH: Smad2 Protein, MESH: Female, MESH: RNA, Messenger, MESH: Cells, Cultured
Abstract: International audience; BACKGROUND: Persistence of myofibroblasts is believed to contribute to the development of fibrosis in idiopathic pulmonary fibrosis (IPF). Transforming growth factor beta1 (TGFbeta1) irreversibly converts fibroblasts into pathological myofibroblasts, which express smooth muscle alpha-actin (alpha-SMA) and produce extracellular matrix proteins, such as procollagen I (alpha1). Reactive oxygen species produced by NADPH oxidases (NOXs) have been shown to regulate cell differentiation. It was hypothesised that NOX could be expressed in parenchymal pulmonary fibroblasts and could mediate TGFbeta1-stimulated conversion of fibroblasts into myofibroblasts. METHODS: Fibroblasts were cultured from the lung of nine controls and eight patients with IPF. NOX4, alpha-SMA and procollagen I (alpha1) mRNA and protein expression, reactive oxygen species production and Smad2/3 phosphorylation were quantified, in the absence and in the presence of incubation with TGFbeta1. Migration of platelet-derived growth factor (PDGF)-induced fibroblasts was also assessed. RESULTS: It was found that (1) NOX4 mRNA and protein expression was upregulated in pulmonary fibroblasts from patients with IPF and correlated with mRNA expression of alpha-SMA and procollagen I (alpha1) mRNA; (2) TGFbeta1 upregulated NOX4, alpha-SMA and procollagen I (alpha1) expression in control and IPF fibroblasts; (3) the change in alpha-SMA and procollagen I (alpha1) expression in response to TGFbeta1 was inhibited by antioxidants and by a NOX4 small interfering RNA (siRNA); (4) NOX4 modulated alpha-SMA and procollagen I (alpha1) expression by controlling activation of Smad2/3; and (5) NOX4 modulated PDGF-induced fibroblast migration. CONCLUSION: NOX4 is critical for modulation of the pulmonary myofibroblast phenotype in IPF, probably by modulating the response to TGFbeta1 and PDGF.
Published: 2010

21. Effect of engineered nanoparticles on vasomotor responses in rat intrapulmonary artery

Author: Etienne Roux, Patrick Brochard, Mireille Canal-Raffin, Arnaud Courtois, Thomas Ducret, Francelyne Marano, Christelle Guibert, Yannick Ladeiro, Françoise Rogerieux, Ghislaine Lacroix, Roger Marthan, Pascal Andujar, Bernard Muller, I. Baudrimont, USC 1366 Oenologie, Institut National de la Recherche Agronomique (INRA), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Génomique Fonctionnelle des Tumeurs Solides (U1162), Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de recherche Cardio-Thoracique de Bordeaux [Bordeaux] (CRCTB), Université Bordeaux Segalen - Bordeaux 2-CHU Bordeaux [Bordeaux]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de l'Environnement Industriel et des Risques (INERIS), Physiopathologie de la réactivite bronchique et vasculaire, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie de la réactivité bronchique et vasculaire, Laboratoire Santé Travail Environnement, Université Bordeaux Segalen - Bordeaux 2-IFR99, CHU Bordeaux [Bordeaux], Université Paris Diderot - Paris 7 (UPD7), Université de Bordeaux Ségalen [Bordeaux 2], Service de pneumologie et pathologie professionnelle, CHI Créteil, Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Laboratoire de physiopathologie de la nutrition (LPN), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Guellaen, Georges, Œnologie, Institut National de la Recherche Agronomique (INRA)-Université Victor Segalen - Bordeaux 2, Unité de Recherche Oenologie [Villenave d'Ornon], and Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)
Subjects: Male, Contraction (grammar), Vasodilator Agents, [SDV]Life Sciences [q-bio], 02 engineering and technology, MESH: Rats, Sprague-Dawley, Toxicology, Endoplasmic Reticulum, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, MESH: Dose-Response Relationship, Drug, Rats, Sprague-Dawley, chemistry.chemical_compound, Vasoconstrictor Agents, MESH: Animals, MESH: Isometric Contraction, ComputingMilieux_MISCELLANEOUS, Titanium, 0303 health sciences, Inhalation Exposure, Vasomotor, Voltage-dependent calcium channel, Biological activity, 021001 nanoscience & nanotechnology, MESH: Titanium, MESH: Calcium Channels, MESH: Inhalation Exposure, 0210 nano-technology, Acetylcholine, medicine.drug, medicine.medical_specialty, Thapsigargin, MESH: Rats, MESH: Pulmonary Artery, chemistry.chemical_element, MESH: Carbon, Calcium, Pulmonary Artery, 03 medical and health sciences, MESH: Vasoconstrictor Agents, In vivo, MESH: Endoplasmic Reticulum, Internal medicine, Isometric Contraction, medicine, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Rats, Wistar, 030304 developmental biology, Pharmacology, Dose-Response Relationship, Drug, MESH: Rats, Wistar, Carbon, MESH: Male, Rats, MESH: Vasodilator Agents, Endocrinology, chemistry, Nanoparticles, Calcium Channels, MESH: Nanoparticles, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Pulmonary circulation could be one of the primary vascular targets of finest particles that can deeply penetrate into the lungs after inhalation. We investigated the effects of engineered nanoparticles on vasomotor responses of small intrapulmonary arteries using isometric tension measurements. Acute in vitro exposure to carbon nanoparticles (CNP) decreased, and in some case abolished, the vasomotor responses induced by several vasoactive agents, whereas acute exposure to titanium dioxide nanoparticles (TiO(2)NP) did not. This could be attributed to a decrease in the activity of those vasoactive agents (including PGF(2)(alpha), serotonin, endothelin-1 and acetylcholine), as suggested when they were exposed to CNP before being applied to arteries. Also, CNP decreased the contraction induced by 30 mM KCl, without decreasing its activity. After endoplasmic reticulum calcium stores depletion (by caffeine and thapsigargin), CaCl(2) addition induced a contraction, dependent on Store-Operated Calcium Channels that was not modified by acute CNP exposure. Further addition of 30 mM KCl elicited a contraction, originating from activation of Voltage-Operated Calcium Channels that was diminished by CNP. Contractile responses to PGF(2)(alpha) or KCl, and relaxation to acetylcholine were modified neither in pulmonary arteries exposed in vitro for prolonged time to CNP or TiO(2)NP, nor in those removed from rats intratracheally instilled with CNP or TiO(2)NP. In conclusion, prolonged in vitro or in vivo exposure to CNP or TiO(2)NP does not affect vasomotor responses of pulmonary arteries. However, acute exposure to CNP decreases contraction mediated by activation of Voltage-Operated, but not Store-Operated, Calcium Channels. Moreover, interaction of some vasoactive agents with CNP decreases their biological activity that might lead to misinterpretation of experimental data.
Published: 2010

22. Niveaux, Déterminants et Variabilité des nanoparticules dans l'environnement intérieur - Projet NANOP

Author: Mandin, Corinne, Ji, Xiaolin, Le Bihan, Olivier, Ramalho, Olivier, D'ANNA, Barbara, Martinon, Laurent, Nicolas, Mélanie, Larbre, Juliette, Bard, Denis, Pairon, Jean-Claude, Institut National de l'Environnement Industriel et des Risques (INERIS), Centre Scientifique et Technique du Bâtiment (CSTB), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut de recherches sur la catalyse et l'environnement de Lyon (IRCELYON), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Etude des Particules Inhalées (LEPI), Ville de Paris, ENSP, École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Pneumologie et Pathologie Professionnelle, CHI Créteil, Institut Interuniversitaire de Médecine du Travail de Paris-Ile de France, Université Biomédicale des Saints-Pères, Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), and Ramalho, Olivier
Subjects: [SDE] Environmental Sciences, Environnement intérieur, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, [SDE]Environmental Sciences, Nanoparticules, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Projet NANOP
Abstract: http://www.afsset.fr/index.php?pageid=2530&parentid=631; National audience; Niveaux, Déterminants et Variabilité des nanoparticules dans l'environnement intérieur - Projet NANOP
Published: 2010

23. Characterisation of particles emitted by incense burning in an experimental house

Author: Jean-Claude Pairon, Xiaolin Ji, Corinne Mandin, Olivier Ramalho, O. Le Bihan, Barbara D'Anna, Mélanie Nicolas, Denis Bard, Laurent Martinon, Institut National de l'Environnement Industriel et des Risques (INERIS), Centre Scientifique et Technique du Bâtiment (CSTB), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut de recherches sur la catalyse et l'environnement de Lyon (IRCELYON), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Laboratoire d'Etude des Particules Inhalées (LEPI), Ville de Paris, ENSP, École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Pneumologie et Pathologie Professionnelle, CHI Créteil, Institut Interuniversitaire de Médecine du Travail de Paris-Ile de France, Université Biomédicale des Saints-Pères, and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Incense, Environmental Engineering, Materials science, 010504 meteorology & atmospheric sciences, Analytical chemistry, Chemical composition, Specific surface area, 010501 environmental sciences, 01 natural sciences, Smoke, Ultrafine particle, Humans, Particle Size, Ceremonial Behavior, 0105 earth and related environmental sciences, Public Health, Environmental and Occupational Health, Environmental engineering, Building and Construction, Particulates, Aerosol, Ultrafine particles, 13. Climate action, Air Pollution, Indoor, [SDE]Environmental Sciences, Housing, Particle, Particle size, Dispersion (chemistry), Particulate matter
Abstract: The potential health effects of fine and ultrafine particles are of increasing concern. A better understanding of particle characteristics and dispersion behavior is needed. This study aims at characterizing spatial and temporal variations in fine and ultrafine particle dispersion after emission from a model source in an experimental house. Particles emitted by an incense stick burning for 15 min were characterized. Number concentration, specific surface area and mass were measured. Partial chemical analysis of particles was also realized. Near the burning incense stick, the maximum concentration was 25,500 particles/cm(3); the indoor PM(2.5) concentration reached 197 microg/m(3), and the specific surface area concentration was 180 microm(2)/cm(3). The estimated incense smoke density was 1.1 g/cm(3). Time of Flight Aerosol Mass Spectrometer measurements indicated that the organic fraction was predominant in the aerosol mass detected, and other minor components identified were K(+), NO(3)(-), and Cl(-). The combustion of an incense stick in the living room was associated with significant modifications of the concentrations of particles measured in the different rooms of the house. This demonstration of pollution by particle dispersion by a model source of moderate intensity may have significant implications in terms of assessment of indoor exposure to such particles. Practical Implications The particles emitted in a domestic environment by a source of moderate intensity such as burning incense disperse throughout the house, even in rooms with closed doors and in rooms as far away as the next floor. This dispersion has significant implications in terms of evaluating human indoor exposure to fine and ultrafine particles.
Published: 2010

24. Evolution of pleural cancers and malignant pleural mesothelioma incidence in France between 1980 and 2005

Author: Le Stang, Nolwenn, Belot, Aurélien, Gilg Soit Ilg, Anabelle, Rolland, P., Astoul, Philippe, Bara, Simona, Brochard, Patrick, Danzon, Arlette, Delafosse, Patricia, Grosclaude, Pascale, Guizard, Anne-Valérie, Imbernon, Ellen, Lapôtre-Ledoux, Bénédicte, Ligier, Karine, Molinié, Florence, Pairon, Jean-Claude, Sauleau, Erik-André, Trétarre, Brigitte, Velten, Michel, Bossard, Nadine, Goldberg, Marcel, Launoy, Guy, Galateau-Sallé, Françoise, Launoy, Guy, Registre multicentrique à vocation nationale des mésothéliomes pleuraux, Registre Multicentrique à Vocation Nationale des Mésothéliomes Pleuraux (MESONAT), CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-CHU Caen, Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Tumorothèque de Caen Basse-Normandie (TCBN), Cancers et Populations : Facteurs de Risque, Depistage, Pratiques Diagnostiques et Therapeutiques, Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service de Biostatistique, Hospices Civils de Lyon (HCL), Biostatistiques santé, Département biostatistiques et modélisation pour la santé et l'environnement [LBBE], Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Département des maladies chroniques et traumatismes, Institut de Veille Sanitaire (INVS), Département santé travail (DST-InVS), Division d'oncologie thoracique, Département des maladies pulmonaires, Université de la Méditerranée - Aix-Marseille 2-Assistance Publique - Hôpitaux de Marseille (APHM)-Hôpital Sainte-Marguerite [CHU - APHM] (Hôpitaux Sud ), FRANCIM, Réseau des registres français du cancer, Laboratoire Santé Travail Environnement, Université Bordeaux Segalen - Bordeaux 2-IFR99, Service de Médecine du Travail et de Pathologies Professionnelles, CHU Bordeaux [Bordeaux], Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de pneumologie et pathologie professionnelle, CHI Créteil, Center for Neurologic Diseases, and Brigham and Women's Hospital [Boston]
Subjects: Male, Mesothelioma, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Incidence, Pleural Neoplasms, Humans, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Female, France, Registries, respiratory system, neoplasms, respiratory tract diseases
Abstract: International audience; The evolution of pleural cancers and malignant pleural mesothelioma incidence in France between 1980 and 2005 was analysed using data derived from the French network of cancer registries (FRANCIM) and the French National Mesothelioma Surveillance Program (PNSM). Mesothelioma proportions in pleural cancers were calculated by diagnosis year in the 1980-2000 period. Our results suggest that the incidences of pleural cancer and mesothelioma levelled off in French men since 2000 and continued to increase in French women. A decrease of the annual pleural cancer incidence average in men was noticed (-3.4% of annual rate of change) between 2000 and 2005. The proportion of pleural cancers that were mesothelioma was unchanged between 1980 and 2003 with an average of 86%. The age standardised incidence rate of pleural mesothelioma remained relatively stable between 1998 and 2005 with a slight falling trend. For women, the age standardised incidence rate of pleural cancers and mesothelioma increased during the period 1998-2005. Additionally, the proportion of pleural cancers that were mesothelioma increased during the same period of time. Finally, the increased trend observed in the incidence of pleural mesothelioma and cancers in women is credibly due to their under diagnosis in the 1980-1997 period. The comparison between the French incidence and the American and British ones shows that the decreasing trend in incidence of mesothelioma and pleural cancers in French men since 2000 is potentially associated with a lower amphibole consumption and by the implementation of safety regulations at work from 1977. (c) 2009 UICC.
Published: 2009

25. Effets respiratoires des nanoparticules

Author: Sophie Lanone, Jorge Boczkowski, Patrick Brochard, Pascal Andujar, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Pneumologie et Pathologie Professionnelle, CHI Créteil, Laboratoire Santé Travail Environnement, Université Bordeaux Segalen - Bordeaux 2-IFR99, and Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)
Subjects: Pulmonary and Respiratory Medicine, Lung Diseases, Manufactured Materials, Materials science, Respiratory System, Population, Nanoparticle, Nanotechnology, Carbon nanotube, toxicité, nanoparticules, Nanomaterials, law.invention, nanotubes, law, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, education, education.field_of_study, Inhalation Exposure, Respiration, Foreign Bodies, Applications of nanotechnology, Nanofiber, poumon, nanomatériaux, Nanomedicine, Nanoparticles, Manufactured nanoparticles
Abstract: International audience; Nanotechnology, defined as techniques aimed to design, characterize and produce materials on a nanometer scale, is a fast-growing field today. Nanomaterials are made of nanoobjects (nanoparticles, nanofibers, nanotubes...). The nanoscale confers on these materials their novel, hitherto unknown, chemical and physical properties by the laws of quantum physics which are essentially expressed on this scale. Nanotechnology applications are numerous (e.g., cosmetics, industry and medicine) and they keep growing. We can safely predict that the production and utilization of nanomaterials will increase greatly in the years to come. Nonetheless, the same properties that make these nanomaterials very attractive are a source of concern: there are questions about their potential toxicity, their long-term side effects, and their biodegradability. These questions are based on knowledge of the toxic effects of micrometric particles in air pollution and the fear that these effects will be amplified because of the nanometric size of the new materials. We present in this article a global but not exhaustive summary of current knowledge. We begin by defining lung penetration, deposition, translocation and elimination of nanoparticles. Finally, we consider the respiratory effects of metallic nanoparticles, titanium dioxide nanoparticles in particular, and carbon nanotubes. In vivo and in vitro experimental studies currently available highlight the existence of biological effects of nanoparticles on the respiratory system with generation of oxidative stress, pro-inflammatory and pro-thrombotic effects and the possible development of fibrosis and pulmonary emphysema or DNA damage. A better understanding of the potential biological effects of nanoparticles is required to implement appropriate preventive measures in the workplace and/or in the general population, if this should be necessary.
Published: 2009

26. [Respiratory effects of manufactured nanoparticles.]

Author: Andujar, Pascal, Lanone, Sophie, Brochard, Patrick, Boczkowski, Jorge, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, Pneumologie et Pathologie Professionnelle, CHI Créteil, Laboratoire Santé Travail Environnement, and Université Bordeaux Segalen - Bordeaux 2-IFR99
Subjects: poumon, nanomatériaux, toxicité, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, nanoparticules, nanotubes
Abstract: International audience; Nanotechnology, defined as techniques aimed to design, characterize and produce materials on a nanometer scale, is a fast-growing field today. Nanomaterials are made of nanoobjects (nanoparticles, nanofibers, nanotubes...). The nanoscale confers on these materials their novel, hitherto unknown, chemical and physical properties by the laws of quantum physics which are essentially expressed on this scale. Nanotechnology applications are numerous (e.g., cosmetics, industry and medicine) and they keep growing. We can safely predict that the production and utilization of nanomaterials will increase greatly in the years to come. Nonetheless, the same properties that make these nanomaterials very attractive are a source of concern: there are questions about their potential toxicity, their long-term side effects, and their biodegradability. These questions are based on knowledge of the toxic effects of micrometric particles in air pollution and the fear that these effects will be amplified because of the nanometric size of the new materials. We present in this article a global but not exhaustive summary of current knowledge. We begin by defining lung penetration, deposition, translocation and elimination of nanoparticles. Finally, we consider the respiratory effects of metallic nanoparticles, titanium dioxide nanoparticles in particular, and carbon nanotubes. In vivo and in vitro experimental studies currently available highlight the existence of biological effects of nanoparticles on the respiratory system with generation of oxidative stress, pro-inflammatory and pro-thrombotic effects and the possible development of fibrosis and pulmonary emphysema or DNA damage. A better understanding of the potential biological effects of nanoparticles is required to implement appropriate preventive measures in the workplace and/or in the general population, if this should be necessary.
Published: 2009

27. [Acute and chronic cadmium poisoning]

Author: Andujar, Pascal, Bensefa-Colas, Lynda, Descatha, Alexis, Guellaen, Georges, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Service de pneumologie et pathologie professionnelle, CHI Créteil, Service de pathologie professionnelle, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Santé publique et épidémiologie des déterminants professionnels et sociaux de la santé, Epidémiologie, sciences sociales, santé publique (IFR 69), Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-École des hautes études en sciences sociales (EHESS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-École des hautes études en sciences sociales (EHESS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut National de la Santé et de la Recherche Médicale (INSERM), Unité de pathologie professionnelle, environnementale et d'insertion, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Raymond Poincaré [AP-HP], and Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-École des hautes études en sciences sociales (EHESS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 1 Panthéon-Sorbonne (UP1)-Université Paris-Sud - Paris 11 (UP11)-École des hautes études en sciences sociales (EHESS)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)
Subjects: inorganic chemicals, Cadmium Poisoning, Smoking, Bronchi, osteomalacia, Kidney, poisoning, Foodborne Diseases, Pulmonary Alveoli, Trachea, Intestinal Absorption, Liver, Acute Disease, Administration, Inhalation, Chronic Disease, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, pneumonia, nephropathy, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Cadmium, Half-Life
Abstract: International audience; Cadmium is a metallic impurity in various minerals. The two main cadmium exposure sources in general population are food and tobacco smoking. Its industrial exploitation has grown in the early twentieth century. Cadmium is used in accumulators or alkaline batteries (80%) and in pigments for paints or plastics (10%), in electrolytic process by deposit or by cadmium plating on metals or to reduce melting points (welding rods...). Cadmium is a cumulative toxic substance whose half-time for elimination is about 20 to 40 years and it is mainly stored in the liver and kidneys. Inhalation of cadmium oxide fumes may cause inhalation fevers or chemical pneumonitis. Cadmium chronic poisoning causes mainly renal tubulopathy and could be the cause of osteomalacia and diffuse osteoporosis. Cadmium is classified as certain carcinogen agent for humans by International Agency for Research on Cancer (IARC). The most relevant biological index exposure is the urinary cadmium. According to literature, no chelating agent can be still used in human cadmium poisonings. In France, some diseases caused by occupational exposure to cadmium may be compensated.
Published: 2008

28. Exposure to nanoparticles in residential environment

Author: Ji, Xiaolin, Mandin, Corinne, Ramalho, Olivier, Le Bihan, Olivier, Martinon, Laurent, Bard, Denis, Pairon, Jean-Claude, Institut National de l'Environnement Industriel et des Risques (INERIS), Centre Scientifique et Technique du Bâtiment (CSTB), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Laboratoire d'Etude des Particules Inhalées (LEPI), Ville de Paris, ENSP, École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Pneumologie et Pathologie Professionnelle, CHI Créteil, Institut Interuniversitaire de Médecine du Travail de Paris-Ile de France, Université Biomédicale des Saints-Pères, and Ramalho, Olivier
Subjects: [SDE] Environmental Sciences, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, [SDE]Environmental Sciences, Residential environment, Nanoparticles, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, ComputingMilieux_MISCELLANEOUS, Exposure
Abstract: International audience
Published: 2008

29. Exposition humaine aux particules ultrafines : caractérisation des sources domestiques - projet NANOP

Author: Ji, Xiaolin, Mandin, Corinne, Le Bihan, Olivier, Ramalho, Olivier, Martinon, Laurent, Bard, Denis, Pairon, Jean-Claude, Institut National de l'Environnement Industriel et des Risques (INERIS), Centre Scientifique et Technique du Bâtiment (CSTB), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Laboratoire d'Etude des Particules Inhalées (LEPI), Ville de Paris, ENSP, École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Pneumologie et Pathologie Professionnelle, CHI Créteil, Institut Interuniversitaire de Médecine du Travail de Paris-Ile de France, Université Biomédicale des Saints-Pères, and Ramalho, Olivier
Subjects: [SDE] Environmental Sciences, Sources domestiques, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Particules ultrafines, [SDE]Environmental Sciences, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Projet NANOP
Published: 2008

30. Measuring levels, assessing determinants and variabilities of nanoparticles concentrations in residential environment, The NANOP Project

Author: Mandin, Corinne, Ramalho, Olivier, Le Bihan, Olivier, Pairon, Jean-Claude, Martinon, Laurent, Bard, Denis, Institut National de l'Environnement Industriel et des Risques (INERIS), Centre Scientifique et Technique du Bâtiment (CSTB), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Pneumologie et Pathologie Professionnelle, CHI Créteil, Institut Interuniversitaire de Médecine du Travail de Paris-Ile de France, Université Biomédicale des Saints-Pères, Laboratoire d'Etude des Particules Inhalées (LEPI), Ville de Paris, ENSP, École des Hautes Études en Santé Publique [EHESP] (EHESP), and Ramalho, Olivier
Subjects: [SDE] Environmental Sciences, [SDE]Environmental Sciences
Abstract: International audience; The NANOP project aims at assessing nanoparticle levels and their spatial and temporal variation in domestic environment simulated within an experimental house (MARIA) based at the Building Technical and Scientific Center (CSTB), i.e. a fully controlled domestic environment (as regards air exchange, heating...). Possible relations between different metrics such as particle number, mass, specific surface, size distribution should be identified. Respective contributions of indoor sources to exposure, but also from outdoor air will be determined.
Published: 2007

31. Exposition précoce à la pollution atmosphérique: Effets respiratoires d’expositions à des épisodes simulés de pollution atmosphérique

Author: Sophie Lanone, INSERM U955, équipe 4, Service de Physiologie Explorations Fonctionnelles, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Service de pneumologie et pathologie professionnelle, Centre Hospitalier Intercommunal de Créteil (CHIC)-Centre Hospitalier Intercommunal de Créteil (CHIC)-Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), and PNR EST
Subjects: aérosol, monoxyde de carbone, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, changement climatique, exposition maternelle, appareil respiratoire, pollution atmosphérique, autophagie, chambre simulation, particule fine, souris, stress oxydant, ozone, gestation, synergie, inflammation, oxyde azote, vulnérabilité, qualité air, dioxyde de soufre
Abstract: National audience; À travers la simulation d’atmosphères réalistes complexes, l’objectif principal du projet PolluHealth est d’évaluer les effets respiratoires, sur la descendance, d’une exposition maternelle à la pollution atmosphérique pendant la gestation. Notre étude originale vise à : 1.Générer et caractériser trois types d’atmosphères polluées complexes (Paris, Pékin et une ville méditerranéenne du futur) / 2.Évaluer les conséquences respiratoires de l’exposition de souris gestantes à ces trois types d’atmosphères sur leur descendance.

32. Assessment of lung hyperinflation in occupational chronic obstructive pulmonary disease: a multicentric cross-sectional study.

Author: de Broucker V, Andujar P, Wardyn PM, Lepage N, Le Rouzic O, Edmé JL, and Hulo S
Subjects: Humans, Lung, Cross-Sectional Studies, Quality of Life, Pulmonary Disease, Chronic Obstructive, Occupational Exposure adverse effects
Abstract: Occupational exposure is associated with elevated morbidity and lower quality of life in patients with chronic obstructive pulmonary disease (COPD). Static hyperinflation is an independent risk factor for all-cause mortality in COPD and for COPD exacerbation. In a multicentre, cross-sectional study (BPROFETIO), we sought to analyse the relationship between static hyperinflation and occupational exposure in patients with COPD with or without occupational exposure., Material and Methods: An overall 'whole working life' cumulative exposure index was calculated for occupational patients with COPD. Spirometry indices and lung volumes were measured according to the 2005 American Thoracic Society/European Respiratory Society guidelines., Results: After adjustment for age, sex, height, body mass index, smoking and coexposure, the analysis for each occupational hazard showed a higher risk for hyperinflation and FEV 1 decline or progression of COPD or GOLD stage for patients with COPD exposed to non-metallic inorganic dusts., Conclusion: Occupational exposures should be more investigated in clinical practice and studies as they contribute to the COPD heterogeneity and are associated for some with the development of a static hyperinflation; a condition that is known to have a negative impact on quality of life and survival., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
Published: 2023
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33. Head and neck cancer and asbestos exposure.

Author: Clin B, Gramond C, Thaon I, Brochard P, Delva F, Chammings S, Gislard A, Laurent F, Paris C, Lacourt A, and Pairon JC
Subjects: Follow-Up Studies, Humans, Male, Asbestos adverse effects, Head and Neck Neoplasms epidemiology, Head and Neck Neoplasms etiology, Laryngeal Neoplasms epidemiology, Laryngeal Neoplasms etiology, Lung Neoplasms, Occupational Exposure adverse effects, Pleural Diseases epidemiology
Abstract: Objectives: The aim of this study was to analyse, within a French cohort of workers previously occupationally exposed to asbestos, incidence and mortality from various sites of head and neck cancers (larynx excluded) and to examine the potential link of these cancers with pleural plaques., Methods: A 10-year follow-up study was conducted in the 13 481 male subjects included in the cohort between October 2003 and December 2005. Asbestos exposure was assessed by industrial hygienist analysis of a standardised questionnaire. The final cumulative exposure index (CEI; in equivalent fibres.years/mL) for each subject was calculated as the sum of each employment period's four-level CEI. The number of head and neck cancers recorded by the National Health Insurance fund was collected in order to conduct an incidence study. Complementary analysis was restricted to men who had performed at least one chest CT scan (N=4804). A mortality study was also conducted. We used a Cox model with age as the time axis variable adjusted for smoking, time since first exposure, CEI of exposure to asbestos and pleural plaques on CT scans., Results: We reported a significant dose-response relationship between CEI of exposure to asbestos and head and neck cancers after exclusion of laryngeal cancers, in the mortality study (HR 1.03, 95% CI (1.01 to 1.06) for an increase of 10 f.years/mL) and a close to significant dose-response relationship in the incidence study (HR 1.02, 95% CI (1.00 to 1.04) for an increase of 10 f.years/mL). No statistically significant association between pleural plaques and head and neck cancer incidence was observed., Conclusions: This large-scale study suggests a relationship between asbestos exposure and head and neck cancers, after exclusion of laryngeal cancers, regardless of whether associated pleural plaques were present., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
Published: 2022
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34. Short and long-term outcomes of patients with COVID-19-associated acute respiratory distress syndrome and difficult veno-venous-ECMO weaning.

Author: Masi P, Tuffet S, Boyer L, Folliguet T, Mekontso Dessap A, and de Prost N
Subjects: Aged, COVID-19 mortality, Cohort Studies, Female, Humans, Male, Middle Aged, Respiratory Distress Syndrome mortality, Respiratory Rate, Retrospective Studies, Tidal Volume, COVID-19 therapy, Extracorporeal Membrane Oxygenation, Respiratory Distress Syndrome therapy, Ventilator Weaning
Published: 2021
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35. Beclin-1 increases with obstructive sleep apnea severity.

Author: Schlemmer F, Zysman M, Ribeiro Baptista B, Audureau E, Covali Noroc A, Ridoux A, Derumeaux G, Adnot S, Maitre B, le Corvoisier P, Lanone S, Boczkowski J, and Boyer L
Subjects: Animals, Autophagy, Beclin-1, Biomarkers, Humans, Hypoxia, Sleep Apnea, Obstructive complications
Abstract: Obstructive sleep apnea is a common chronic disorder that leads to chronic intermittent hypoxia described as an important factor contributing to the pathogenesis of OSA-related comorbidities. Besides, recent data suggest that intermittent hypoxia can induce adaptative cardiovascular pathways inducing a relative resistance to ischemic insults. Adaptative pathways induced by hypoxia could implicate autophagic processes and Beclin-1, one of the first mammalian autophagy effectors. Thus, activation of autophagy could protect against cardiovascular events in patients with OSA and could be considered as biomarker of a better prognosis., (Copyright © 2021. Published by Elsevier B.V.)
Published: 2021
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36. Genetic alterations of malignant pleural mesothelioma: association with tumor heterogeneity and overall survival.

Author: Quetel L, Meiller C, Assié JB, Blum Y, Imbeaud S, Montagne F, Tranchant R, de Wolf J, Caruso S, Copin MC, Hofman V, Gibault L, Badoual C, Pintilie E, Hofman P, Monnet I, Scherpereel A, Le Pimpec-Barthes F, Zucman-Rossi J, Jaurand MC, and Jean D
Subjects: Adult, Aged, Aged, 80 and over, Cell Line, Tumor, Female, Humans, Kaplan-Meier Estimate, Male, Mesothelioma, Malignant epidemiology, Mesothelioma, Malignant pathology, Middle Aged, Mutation genetics, Pleural Neoplasms epidemiology, Pleural Neoplasms pathology, Survival Analysis, Young Adult, Genetic Heterogeneity, Mesothelioma, Malignant genetics, Pleural Neoplasms genetics
Abstract: Development of precision medicine for malignant pleural mesothelioma (MPM) requires a deep knowledge of tumor heterogeneity. Histologic and molecular classifications and histo-molecular gradients have been proposed to describe heterogeneity, but a deeper understanding of gene mutations in the context of MPM heterogeneity is required and the associations between mutations and clinical data need to be refined. We characterized genetic alterations on one of the largest MPM series (266 tumor samples), well annotated with histologic, molecular and clinical data of patients. Targeted next-generation sequencing was performed focusing on the major MPM mutated genes and the TERT promoter. Molecular heterogeneity was characterized using predictors allowing classification of each tumor into the previously described molecular subtypes and the determination of the proportion of epithelioid-like and sarcomatoid-like components (E/S.scores). The mutation frequencies are consistent with literature data, but this study emphasized that TERT promoter, not considered by previous large sequencing studies, was the third locus most affected by mutations in MPM. Mutations in TERT promoter, NF2, and LATS2 were more frequent in nonepithelioid MPM and positively associated with the S.score. BAP1, NF2, TERT promoter, TP53, and SETD2 mutations were enriched in some molecular subtypes. NF2 mutation rate was higher in asbestos unexposed patient. TERT promoter, NF2, and TP53 mutations were associated with a poorer overall survival. Our findings lead to a better characterization of MPM heterogeneity by identifying new significant associations between mutational status and histologic and molecular heterogeneity. Strikingly, we highlight the strong association between new mutations and overall survival., (© 2020 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.)
Published: 2020
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37. [Outdoor pollution and its effects on lung health in France. Expert document from the Groupe Pathologies pulmonaires professionnelles environnementales et iatrogéniques (PAPPEI) of the Société de pneumologie de langue française (SPLF)].

Author: Caillaud D, Annesi-Maesano I, Bourin A, Chinet T, Colette A, De Blay F, Dixsaut G, Housset B, Kleinpeter J, Malherbe L, Roussel I, Dalphin JC, and Charpin D
Subjects: Climate Change, Environmental Illness epidemiology, Environmental Monitoring methods, Environmental Monitoring standards, Environmental Pollution analysis, Environmental Pollution prevention & control, Environmental Pollution statistics & numerical data, France epidemiology, Government Regulation, Humans, Iatrogenic Disease, Legislation, Medical organization & administration, Legislation, Medical standards, Legislation, Medical trends, Lung Diseases epidemiology, Occupational Diseases epidemiology, Occupational Diseases prevention & control, Pulmonary Medicine methods, Pulmonary Medicine organization & administration, Pulmonary Medicine trends, Societies, Medical organization & administration, Societies, Medical standards, Environmental Illness etiology, Environmental Pollution adverse effects, Lung Diseases etiology, Occupational Diseases etiology, Pulmonary Medicine standards
Published: 2019
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38. LUCSO-1-French pilot study of LUng Cancer Screening with low-dose computed tomography in a smokers population exposed to Occupational lung carcinogens: study protocol.

Author: Delva F, Laurent F, Paris C, Belacel M, Brochard P, Bylicki O, Chouaïd C, Clin B, Dewitte JD, Le Denmat V, Gehanno JF, Lacourt A, Margery J, Verdun-Esquer C, Mathoulin-Pélissier S, and Pairon JC
Subjects: Aged, Female, France, Humans, Lung Neoplasms epidemiology, Male, Middle Aged, Occupational Exposure analysis, Pilot Projects, Radiation Dosage, Risk Assessment, Risk Factors, Tomography, X-Ray Computed methods, Carcinogens analysis, Early Detection of Cancer methods, Lung Neoplasms diagnosis, Occupational Exposure adverse effects, Smokers, Tomography, X-Ray Computed adverse effects
Abstract: Introduction: Guidelines concerning the follow-up of subjects occupationally exposed to lung carcinogens, published in France in 2015, recommended the setting up of a trial of low-dose chest CT lung cancer screening in subjects at high risk of lung cancer., Objective: To evaluate the organisation of low-dose chest CT lung cancer screening in subjects occupationally exposed to lung carcinogens and at high risk of lung cancer., Methods and Analysis: This trial will be conducted in eight French departments by six specialised reference centres (SRCs) in occupational health. In view of the exploratory nature of this trial, it is proposed to test initially the feasibility and acceptability over the first 2 years in only two SRCs then in four other SRCs to evaluate the organisation. The target population is current or former smokers with more than 30 pack-years (who have quit smoking for less than 15 years), currently or previously exposed to International Agency for Research on Cancer group 1 lung carcinogens, and between the ages of 55 and 74 years. The trial will be conducted in the following steps: (1) identification of subjects by a screening invitation letter; (2) evaluation of occupational exposure to lung carcinogens; (3) evaluation of the lung cancer risk level and verification of eligibility; (4) screening procedure: annual chest CT scans performed by specialised centres and (5) follow-up of CT scan abnormalities., Ethics and Dissemination: This protocol study has been approved by the French Committee for the Protection of Persons. The results from this study will be submitted to peer-reviewed journals and reported at suitable national and international meetings., Trial Registration Number: NCT03562052; Pre-results., Competing Interests: Competing interests: OB declares competing interest with MSD and Astra-Zeneca. VLD declares competing interest with GSK and Pierre Fabre. Other authors declare that they have no competing interest., (© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
Published: 2019
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39. [Respiratory isolation in suspected tuberculosis with negative direct sputum examination].

Author: Pichon J, Chouaid C, Marc E, and Voiriot G
Subjects: Air Microbiology, Environment, Controlled, False Negative Reactions, Humans, Mycobacterium tuberculosis genetics, Mycobacterium tuberculosis isolation & purification, Patient Isolation methods, Public Health methods, Infection Control methods, Respiration, Sputum microbiology, Tuberculosis diagnosis, Tuberculosis therapy
Abstract: Airborne isolation is the main confinement measure used to limit human-to-human transmission of tuberculosis. If implemented early, precisely as soon as the patient is clinically diagnosed with tuberculosis, this measure will protect the population, particularly the health workers who are exposed. A patient suspected of being infected with tuberculosis can create a difficult situation if microbiological examination of his respiratory secretions is negative. This is a complex laboratory technique and sensitivity varies from one test to another. Thus, a false negative result is possible; meaning that a patient can have positive results on a microbiological culture performed later. This patient would still have low, but not no, contagiousness as long as a treatment has not been initiated. This situation can extend the period of respiratory isolation while further diagnostic investigations are carried out. This extended isolation can reduce the quality of health care delivered and patients can show signs of depression and anxiety. The use in routine clinical investigation of gene amplification tools should allow a rethinking of respiratory isolation rules. These tools, which are very sensitive and with a short reporting time, could drastically reduce the duration of respiratory isolation for patients suspected of being infected with tuberculosis., (Copyright © 2019 SPLF. Published by Elsevier Masson SAS. All rights reserved.)
Published: 2019
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40. Inflammatory Cellular Response to Mechanical Ventilation in Elastase-Induced Experimental Emphysema: Role of Preexisting Alveolar Macrophages Infiltration.

Author: Rouze A, Voiriot G, Guivarch E, Roux F, Tran Van Nhieu J, Isabey D, Brochard L, Maitre B, Mekontso-Dessap A, and Boczkowski J
Subjects: Animals, Bronchoalveolar Lavage Fluid cytology, Disease Models, Animal, Inflammation metabolism, Lung metabolism, Lung pathology, Macrophages, Alveolar metabolism, Male, Mice, Mice, Inbred C57BL, Monocytes metabolism, Monocytes pathology, Neutrophils metabolism, Neutrophils pathology, Pneumonia metabolism, Pneumonia pathology, Pulmonary Emphysema metabolism, Swine, Ventilators, Mechanical, Inflammation pathology, Macrophages, Alveolar pathology, Pancreatic Elastase metabolism, Pulmonary Emphysema pathology
Abstract: An excessive pulmonary inflammatory response could explain the poor prognosis of chronic obstructive pulmonary disease (COPD) patients submitted to invasive mechanical ventilation. The aim of this study was to evaluate the response to normal tidal volume mechanical ventilation in an elastase-induced murine model of pulmonary emphysema. In this model, two time points, associated with different levels of lung inflammation but similar lung destruction, were analyzed. C57BL/6 mice received a tracheal instillation of 5 IU of porcine pancreatic elastase (Elastase mice) or the same volume of saline (Saline mice). Fourteen (D14) and 21 (D21) days after instillation, mice were anesthetized, intubated, and either mechanically ventilated (MV) or maintained on spontaneous ventilation (SV) during two hours. As compared with Saline mice, Elastase mice showed a similarly increased mean chord length and pulmonary compliance at D14 and D21, while bronchoalveolar lavage cellularity was comparable between groups. Lung mechanics was similarly altered during mechanical ventilation in Elastase and Saline mice. Activated alveolar macrophages CD11b mid were present in lung parenchyma in both Elastase SV mice and Elastase MV mice at D14 but were absent at D21 and in Saline mice, indicating an inflammatory state with elastase at D14 only. At D14, Elastase MV mice showed a significant increase in percentage of neutrophils in total lung, as compared with Elastase SV mice. Furthermore, alveolar macrophages of Elastase MV mice at D14 overexpressed Gr1, and monocytes showed a trend to overexpression of CD62L, compared with Elastase SV mice. In an elastase-induced model of pulmonary emphysema, normal tidal volume mechanical ventilation may produce an increase in the proportion of pulmonary neutrophils, and an activation of alveolar macrophages and pulmonary monocytes. This response seems to be observed only when the emphysema model shows an underlying inflammation (D14), reflected by the presence of activated alveolar macrophages CD11b mid.
Published: 2018
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41. Are Systemic Manifestations Ascribable to COPD in Smokers? A Structural Equation Modeling Approach.

Author: Boyer L, Bastuji-Garin S, Chouaid C, Housset B, Le Corvoisier P, Derumeaux G, Boczkowski J, Maitre B, Adnot S, and Audureau E
Subjects: Aged, Female, Humans, Inflammation blood, Inflammation physiopathology, Leukocyte Count, Lung pathology, Lung physiopathology, Male, Middle Aged, Models, Biological, Prospective Studies, Pulmonary Disease, Chronic Obstructive blood, Pulse Wave Analysis, Respiratory Function Tests, Vascular Stiffness, Non-Smokers statistics & numerical data, Pulmonary Disease, Chronic Obstructive physiopathology, Smokers statistics & numerical data
Abstract: Whether the systemic manifestations observed in Chronic Obstructive Pulmonary Disease (COPD) are ascribable to lung dysfunction or direct effects of smoking is in debate. Structural Equations Modeling (SEM), a causal-oriented statistical approach, could help unraveling the pathways involved, by enabling estimation of direct and indirect associations between variables. The objectives of the study was to investigate the relative impact of smoking and COPD on systemic manifestations, inflammation and telomere length. In 292 individuals (103 women; 97 smokers with COPD, 96 smokers without COPD, 99 non-smokers), we used SEM to explore the pathways between smoking (pack-years), lung disease (FEV 1 , K CO ), and the following parameters: arterial stiffness (aortic pulse wave velocity, PWV), bone mineral density (BMD), appendicular skeletal muscle mass (ASMM), grip strength, insulin resistance (HOMA-IR), creatinine clearance (eGFR), blood leukocyte telomere length and inflammatory markers (Luminex assay). All models were adjusted on age and gender. Latent variables were created for systemic inflammation (inflammatory markers) and musculoskeletal parameters (ASMM, grip strength, BMD). SEM showed that most effects of smoking were indirectly mediated by lung dysfunction: e.g. via FEV 1 on musculoskeletal factor, eGFR, HOMA-IR, PWV, telomere length, CRP, white blood cells count (WBC) and inflammation factor, and via K CO on musculoskeletal factor, eGFR and PWV. Direct effects of smoking were limited to CRP and WBC. Models had excellent fit. In conclusion, SEM highlighted the major role of COPD in the occurrence of systemic manifestations while smoking effects were mostly mediated by lung function.
Published: 2018
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42. Smoking, telomere length and lung function decline: a longitudinal population-based study.

Author: Andujar P, Courbon D, Bizard E, Marcos E, Adnot S, Boyer L, Demoly P, Jarvis D, Neukirch C, Pin I, Thabut G, Boczkowski J, and Leynaert B
Subjects: Adult, Biomarkers metabolism, Cross-Sectional Studies, Female, Follow-Up Studies, Forced Expiratory Volume genetics, France, Health Surveys, Humans, Leukocytes, Longitudinal Studies, Male, Risk Factors, Spirometry methods, Telomere Homeostasis, Telomere Shortening, Young Adult, Forced Expiratory Volume physiology, Lung physiopathology, Pulmonary Disease, Chronic Obstructive genetics, Smoking adverse effects, Telomere metabolism
Abstract: Telomere shortening is associated with COPD and impaired lung function in cross-sectional studies, but there is no longitudinal study. We used data from 448 participants recruited as part of the French follow-up of the European Community Respiratory Health Survey. We found no relationship between telomere length at baseline and FEV 1 decline after 11 years of follow-up. However, heavy smoking was associated with an accelerated FEV 1 decline in individuals with short telomeres, but not in subjects with longer telomeres (p for interaction p=0.08). Our findings suggest that short telomere length in peripheral leucocytes might be a marker for increased susceptibility to the effect of smoking., Competing Interests: Competing interests: None declared., (© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2018. All rights reserved. No commercial use is permitted unless otherwise expressly granted.)
Published: 2018
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43. Beclin1 circulating levels and accelerated aging markers in COPD.

Author: Schlemmer F, Boyer L, Soumagne T, Ridoux A, Chouaid C, Maitre B, Lanone S, Adnot S, Audureau E, and Boczkowski J
Subjects: Biomarkers, Female, Humans, Male, Middle Aged, Aging blood, Beclin-1 blood, Pulmonary Disease, Chronic Obstructive blood
Published: 2018
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44. Respiratory effects of trichloroethylene.

Author: Dumas O, Despreaux T, Perros F, Lau E, Andujar P, Humbert M, Montani D, and Descatha A
Subjects: Chronic Disease, Humans, Lung Neoplasms chemically induced, Lung Neoplasms epidemiology, Occupational Diseases epidemiology, Occupational Exposure adverse effects, Pulmonary Veno-Occlusive Disease chemically induced, Pulmonary Veno-Occlusive Disease epidemiology, Respiration Disorders epidemiology, Solvents pharmacokinetics, Trichloroethylene pharmacokinetics, Occupational Diseases chemically induced, Respiration Disorders chemically induced, Solvents adverse effects, Trichloroethylene adverse effects
Abstract: Trichloroethylene (TCE) is a chlorinated solvent that has been used widely around the world in the twentieth century for metal degreasing and dry cleaning. Although TCE displays general toxicity and is classified as a human carcinogen, the association between TCE exposure and respiratory disorders are conflicting. In this review we aimed to systematically evaluate the current evidence for the respiratory effects of TCE exposure and the implications for the practicing clinician. There is limited evidence of an increased risk of lung cancer associated with TCE exposure based on animal and human data. However, the effect of other chlorinated solvents and mixed solvent exposure should be further investigated. Limited data are available to support an association between TCE exposure and respiratory tract disorders such as asthma, chronic bronchitis, or rhinitis. The most consistent data is the association of TCE with autoimmune and vascular diseases such as systemic sclerosis and pulmonary veno-occlusive disease. Although recent data are reassuring regarding the absence of an increased lung cancer risk with TCE exposure, clinicians should be aware of other potential respiratory effects of TCE. In particular, occupational exposure to TCE has been linked to less common conditions such as systemic sclerosis and pulmonary veno-occlusive disease., (Copyright © 2017. Published by Elsevier Ltd.)
Published: 2018
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45. Prevalence of occupational exposure to asbestos and crystalline silica according to phenotypes of lung cancer from the CaProMat study: A case-only study.

Author: El Zoghbi M, Salameh P, Stücker I, Paris C, Pairon JC, Gislard A, Siemiatycki J, Bonneterre V, Clin B, Brochard P, Delva F, and Lacourt A
Subjects: Asbestos toxicity, France epidemiology, Humans, Lung Neoplasms etiology, Male, Middle Aged, Occupational Diseases etiology, Occupational Exposure adverse effects, Prevalence, Quebec epidemiology, Silicon Dioxide toxicity, Asbestos analysis, Lung Neoplasms epidemiology, Occupational Diseases epidemiology, Occupational Exposure statistics & numerical data, Phenotype, Silicon Dioxide analysis
Abstract: Background: The objective of the study was to compare the prevalence of occupational exposure to asbestos and crystalline silica according to histological types of lung cancer and age at diagnosis., Methods: CaProMat study is a pooled case-only study conducted between 1996 and 2011. The current study consisted of 6521 lung cancer cases. Occupational exposure to asbestos and crystalline silica was assessed by two Job-Exposure Matrices. A weighted prevalence of exposure was derived and compared according to histological types and age at diagnosis., Results: There was no difference of weighted prevalence of exposure to asbestos and crystalline silica according to histological types of lung cancer. There was a statistically significant difference of weighted prevalence of exposure to asbestos and crystalline silica according to age at diagnosis., Conclusions: Due to the limited clinical importance of the difference, neither the histological type, nor the age at diagnosis can be used as an indicator for the occupational exposure to asbestos or crystalline silica., (© 2017 Wiley Periodicals, Inc.)
Published: 2018
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46. Impaired humoral and cellular immune responses to influenza vaccination in chronic obstructive pulmonary disease patients.

Author: Parpaleix A, Boyer L, Wiedemann A, Lacabaratz C, Margarit L, Enouf V, Le Corvoisier P, Lino A, Covali-Noroc A, Housset B, Chouaid C, Maitre B, Lévy Y, Lelièvre JD, and Adnot S
Subjects: B-Lymphocytes immunology, Female, Humans, Immunity, Cellular, Immunity, Humoral, Influenza A Virus, H1N1 Subtype immunology, Influenza A Virus, H3N2 Subtype immunology, Influenza B virus immunology, Influenza, Human prevention & control, Male, Middle Aged, T-Lymphocytes immunology, Vaccination, Influenza Vaccines, Pulmonary Disease, Chronic Obstructive immunology
Published: 2017
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47. Cancer of the esophagus and asbestos exposure.

Author: Clin B, Thaon I, Boulanger M, Brochard P, Chamming's S, Gislard A, Lacourt A, Luc A, Ogier G, Paris C, and Pairon JC
Subjects: Aged, Esophageal Neoplasms mortality, Follow-Up Studies, France epidemiology, Humans, Incidence, Male, Middle Aged, Occupational Diseases mortality, Occupational Exposure statistics & numerical data, Asbestos toxicity, Carcinogens toxicity, Esophageal Neoplasms epidemiology, Occupational Diseases epidemiology, Occupational Exposure adverse effects
Abstract: Background: Our study aimed at analyzing incidence and mortality from esophageal cancer within a cohort of workers with previous occupational asbestos exposure (ARDCo Program)., Methods: A 10-year follow-up study was conducted in the 14 515 male subjects included in this program between October 2003 and December 2005. Follow-up began when exposure stopped. Asbestos exposure was analyzed by industrial hygienists using data from a standardized questionnaire. The Cox model was used, with age as the time axis variable adjusted for smoking, time since first exposure (TSFE) and cumulative exposure index (CEI) of exposure to asbestos., Results: We reported a significant dose-response relationship between CEI of exposure to asbestos and esophageal cancer, in both incidence (hazard ratio [HR] 1.26, 95% confidence interval [CI] 1.00-1.58), and mortality (HR 1.40, [95%CI 1.12-1.75])., Conclusions: This large-scale study suggests the existence of a relationship between asbestos exposure and cancer of the esophagus., (© 2017 Wiley Periodicals, Inc.)
Published: 2017
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48. Phenotypes of lung cancer and statistical interactions between tobacco smoking and occupational exposure to asbestos and crystalline silica from a large case-only study: The CaProMat study.

Author: El Zoghbi M, Salameh P, Stücker I, Paris C, Pairon JC, Gislard A, Siemiatycki J, Bonneterre V, Clin B, Brochard P, Delva F, and Lacourt A
Subjects: Aged, Canada epidemiology, Female, Humans, Lung Neoplasms diagnosis, Male, Middle Aged, Occupational Diseases diagnosis, Occupational Diseases epidemiology, Occupational Diseases etiology, Odds Ratio, Population Surveillance, Retrospective Studies, Asbestos adverse effects, Lung Neoplasms epidemiology, Lung Neoplasms etiology, Occupational Exposure adverse effects, Phenotype, Silicon Dioxide adverse effects, Tobacco Smoking adverse effects
Abstract: Objectives: The aim of this study was to assess the effect modification of the association between tobacco smoking and phenotypes of lung cancer (histological type, tumor location, and age at diagnosis) by occupational exposure to asbestos or to crystalline silica., Materials and Methods: The CaProMat study is a pooled case-only study including 7256 male lung cancer cases recruited between 1996 and 2011 in France and Canada. Two job-exposure matrices (JEMs) were used to assess occupational exposure to asbestos and crystalline silica. Statistical interactions between tobacco smoking and occupational exposure to asbestos or crystalline silica were assessed using unconditional logistic regression models for histological type and tumor location and linear regression models for age at diagnosis., Results: Tobacco smoking was associated with squamous cell carcinoma and small cell carcinomas as well as an earlier age at diagnosis. Additional exposure to either asbestos or crystalline silica did not modify the effect of tobacco smoking for either histological type or age at diagnosis. Neither tobacco smoking nor occupational exposure to asbestos or crystalline silica influenced tumor location., Conclusions: Tobacco smoking was the main factor related to histological type and age at diagnosis. Those associations were not modified by occupational exposure to asbestos or crystalline silica., (Copyright © 2017 Elsevier B.V. All rights reserved.)
Published: 2017
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49. Analysis of health economics assessment reports for pharmaceuticals in France - understanding the underlying philosophy of CEESP assessment.

Author: Toumi M, Motrunich A, Millier A, Rémuzat C, Chouaid C, Falissard B, and Aballéa S
Abstract: Background : Despite the guidelines for Economic and Public Health Assessment Committee (CEESP) submission having been available for nearly six years, the dossiers submitted continue to deviate from them, potentially impacting product prices. Objective : to review the reports published by CEESP, analyse deviations from the guidelines, and discuss their implications for the pricing and reimbursement process. Study design : CEESP reports published until January 2017 were reviewed, and deviations from the guidelines were extracted. The frequency of deviations was described by type of methodological concern (minor, important or major). Results : In 19 reports, we identified 243 methodological concerns, most often concerning modelling, measurement and valuation of health states and results presentation and sensitivity analyses; nearly 63% were minor, 33% were important and 4.5% were major. All reports included minor methodological concerns, and 17 (89%) included at least one important and/or major methodological concern. Global major methodological concerns completely invalidated the analysis in seven dossiers (37%). Conclusion : The CEESP submission dossiers fail to adhere to the guidelines, potentially invalidating the health economics analysis and resulting in pricing negotiations. As these negotiations tend to be unfavourable for the manufacturer, the industry should strive to improve the quality of the analyses submitted to CEESP.
Published: 2017
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50. Anxious and depressive symptoms in the French Asbestos-Related Diseases Cohort: risk factors and self-perception of risk.

Author: Mounchetrou Njoya I, Paris C, Dinet J, Luc A, Lighezzolo-Alnot J, Pairon JC, and Thaon I
Subjects: Aged, Anxiety Disorders psychology, Causality, Cohort Studies, Comorbidity, Depressive Disorder psychology, Female, France, Humans, Male, Prevalence, Risk, Risk Factors, Surveys and Questionnaires, Anxiety Disorders epidemiology, Asbestosis epidemiology, Asbestosis psychology, Depressive Disorder epidemiology, Self Concept
Abstract: Background: Asbestos is known to be an independent risk factor for lung and pleural cancers. However, to date, little attention has been paid to the psychological effects of asbestos exposure among exposed subjects. The objectives of this study were to estimate the prevalence of anxious and depressive symptoms among >2000 French participants of the Asbestos-Related Diseases Cohort (ARDCO), 6 years after their inclusion, to identify the risk factors associated with those anxious and depressive symptoms and to evaluate the impact of the asbestos-risk perception., Methods: The ARDCO was constituted in four regions of France between October 2003 and December 2005, by including former asbestos workers. Between 2011 and 2012, participants of the ARDCO program were invited to undergo another chest CT scan 6 years after the previous scan. Participants were asked to complete questionnaires including asbestos exposure assessment, Hospital Anxiety and Depression Scale (HADS), asbestos-risk perception and self-perception of asbestos-related diseases., Results: Among the 2225 participants, 2210 fully completed questionnaires were collected and analyzed. The prevalence of symptoms of probable anxiety and probable depression was 19.7% and 9.9%, respectively. The risk of anxious and depressive symptoms was independently associated with self-perception of the intensity of asbestos exposure, asbestos-risk perception and self-perception of asbestos-related diseases., Conclusion: The results obtained in this large study confirm that previously asbestos-exposed subjects are likely to develop anxious and depressive symptoms. Finally, implications related to the prevention of anxiety and depression among asbestos-exposed workers is discussed., (© The Author 2016. Published by Oxford University Press on behalf of the European Public Health Association. All rights reserved.)
Published: 2017
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