1. Characterization of function and regulation of miR-24-1 and miR-31
- Author
-
Sun, Fenyong, Wang, Jiayi, Pan, Qiuhui, Yu, Yongchun, Zhang, Yue, Wan, Yang, Wang, Ju, Li, Xiaoyan, and Hong, An
- Subjects
- *
GENETIC regulation , *NON-coding RNA , *GENETIC transcription , *MESENCHYME , *STEM cells , *CELL proliferation , *LABORATORY mice , *PROMOTERS (Genetics) - Abstract
Abstract: To date, numerous microRNAs (miRNAs) have been discovered. However, the function of these miRNAs is largely unknown. While our knowledge of miRNA post-transcriptional processing has greatly expanded in recent years, we have a limited understanding of the regulation and transcription of miRNA genes. In this study, we characterized two BMP-2 upregulated miRNAs, miR-24-1 and miR-31, in mesenchymal stem cells and showed their opposing function in controlling cellular proliferation, and adipogenesis. Furthermore, we are the first to identify and characterize mouse intronic miR-23b∼27b∼24-1 and intergenic miR-31 genes. Moreover, we found that pri-miR-23b, pri-miR-27b, and pri-miR-24-1 are transcribed independently and their expression profiles are unique when cells are treated with BMP-2, even though they are located closely together. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF