1. Lobular architecture of human adipose tissue defines the niche and fate of progenitor cells
- Author
-
Christian Dani, A. Remaury, Jean Galitzky, Y. Veeranagouda, A. Briot, Anne Bouloumié, David Estève, M. Didier, Pauline Decaunes, Séverine Ledoux, Nathalie Boulet, Alexia Zakaroff-Girard, C. Belles, Jean-Claude Guillemot, Institut des Maladies Métaboliques et Casdiovasculaires (UPS/Inserm U1297 - I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Sanofi Aventis R&D [Chilly-Mazarin], Hôpital Louis Mourier - AP-HP [Colombes], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), and boulet, nathalie
- Subjects
0301 basic medicine ,Cellular differentiation ,[SDV]Life Sciences [q-bio] ,CD34 ,General Physics and Astronomy ,Adipose tissue ,02 engineering and technology ,chemistry.chemical_compound ,MESH: Transforming Growth Factor beta1 ,MESH: Alkaline Phosphatase ,Adipocyte ,Adipocytes ,MESH: Obesity ,MESH: Nerve Tissue Proteins ,Stem Cell Niche ,lcsh:Science ,Myofibroblasts ,Multidisciplinary ,Adipogenesis ,Stem Cells ,Cell Differentiation ,021001 nanoscience & nanotechnology ,Cell biology ,Extracellular Matrix ,[SDV] Life Sciences [q-bio] ,Adipose Tissue ,0210 nano-technology ,Fat metabolism ,Myofibroblast ,MESH: Adipose Tissue ,MESH: Cell Differentiation ,Stromal cell ,MESH: Myofibroblasts ,MESH: Stem Cell Niche ,Science ,Subcutaneous Fat ,Nerve Tissue Proteins ,MESH: Stem Cells ,Receptors, Nerve Growth Factor ,Biology ,MESH: Extracellular Matrix ,Intra-Abdominal Fat ,General Biochemistry, Genetics and Molecular Biology ,Article ,Transforming Growth Factor beta1 ,03 medical and health sciences ,MESH: Receptors, Nerve Growth Factor ,MESH: Subcutaneous Fat ,Humans ,Obesity ,Progenitor cell ,MESH: Intra-Abdominal Fat ,MESH: Adipocytes ,Progenitor ,MESH: Humans ,General Chemistry ,Alkaline Phosphatase ,030104 developmental biology ,chemistry ,Mesenchymal stem cells ,lcsh:Q ,MESH: Adipogenesis - Abstract
Human adipose tissue (hAT) is constituted of structural units termed lobules, the organization of which remains to be defined. Here we report that lobules are composed of two extracellular matrix compartments, i.e., septa and stroma, delineating niches of CD45−/CD34+/CD31− progenitor subsets characterized by MSCA1 (ALPL) and CD271 (NGFR) expression. MSCA1+ adipogenic subset is enriched in stroma while septa contains mainly MSCA1−/CD271− and MSCA1−/CD271high progenitors. CD271 marks myofibroblast precursors and NGF ligand activation is a molecular relay of TGFβ-induced myofibroblast conversion. In human subcutaneous (SC) and visceral (VS) AT, the progenitor subset repartition is different, modulated by obesity and in favor of adipocyte and myofibroblast fate, respectively. Lobules exhibit depot-specific architecture with marked fibrous septa containing mesothelial-like progenitor cells in VSAT. Thus, the human AT lobule organization in specific progenitor subset domains defines the fat depot intrinsic capacity to remodel and may contribute to obesity-associated cardiometabolic risks., In human adipose tissue (AT), adipocytes are organized into units of lobules. Here the authors identify distinct fibrous septal and stromal compartments within AT lobules that differ in composition and potential between subcutaneous and visceral regions and are disturbed in obesity and metabolic syndrome.
- Published
- 2019