101. Inhibition of Solar UV-Induced Matrix Metalloproteinase (MMP)-1 Expression by Non-Enzymatic Softening Cherry Blossom (Prunus yedoensis) Extract
- Author
-
Pomjoo Lee, Jong-Eun Kim, Han-Seung Shin, Yeong-A Jung, and Jiyoon Lee
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,Prunus × yedoensis ,anti-photoaging ,Human skin ,Plant Science ,Matrix metalloproteinase ,environment and public health ,03 medical and health sciences ,Transactivation ,0302 clinical medicine ,cherry blossom ,functional material ,Ecology, Evolution, Behavior and Systematics ,Ecology ,biology ,Chemistry ,Botany ,biology.organism_classification ,Cell biology ,HaCaT ,030104 developmental biology ,QK1-989 ,030220 oncology & carcinogenesis ,non-enzymatic softening technology ,Phosphorylation ,Petal - Abstract
Cherry blossom (Prunus yedoensis) petals are used as ingredients in many cosmetics. However, despite their use in numerous products, the exact function of cherry blossom petals in cosmetics is unclear. Therefore, we need evidence-based studies to support the labeling claims that are made in cherry blossom products in the cosmetics industry. We investigated the skin anti-aging potential of non-enzymatic softening cherry blossom extract (NES-CBE) in this study. The extract desalinated, to improve its quality such that it can be used as a functional material for the skin. The anti-wrinkle effect of NES-CBE was investigated on human keratinocytes (HaCaT cells) under solar UV (sUV) light exposure. We found that NES-CBE reduced the sUV-induced matrix metalloproteinase (MMP)-1 expression and modulated the transactivation of the activator protein (AP)-1. Furthermore, NES-CBE suppressed the phosphorylation of MEK1/2 and ERK proteins, indicating its regulation of sUV-induced MAPK signaling. Additionally, we observed NES-CBE reduced MMP-1 protein expression in a human skin equivalent model. Taken together, these results suggest that NES-CBE reduces sUV-induced MMP-1 protein expression through reducing AP-1 transactivation via regulation of the MEK1/2-ERK pathway.
- Published
- 2021