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Abstract: 【Objective】Explore the effects of different gradients of microbial fertilizer application rate on sugar beet yield and quality in saline-alkali land in the Yellow River Irrigation Area,to find out the optimal sugar beet microbial fertilizer application rate in saline-alkali land.【Methods】In Bayannur City,Inner Mongolia,from 2020 to 2021,five microbial fertilizer application treatments,including T1（0 kg/hm²）,T2（75 kg/hm²）,T3（150 kg/hm²）,T4（225 kg/hm²）and T5（300 kg/hm²）were set up on the basis of two main cultivation modes of flat cropping and ridge cropping. The effects of different treatments on sugar beet yield and sugar yield were analyzed. Based on the variation law of sugar beet yield and sugar yield,a regression model with the application rate of microbial fertilizer was established. And the optimal application rate of microbial fertilizer was calculated. The accuracy of the model was tested by normalized root mean square error（NRMSE）and 1∶1 histogram between the measured value and the estimated value.【Results】The application of microbial fertilizer in saline-alkali land of the Yellow River Irrigation Area significantly improved sugar beet yield and sugar yield. In 2020,T3 treatment had the highest sugar beet yields both in flat cropping and ridge cropping at 71 454 and 73 835 kg/hm²,respectively,increased by 24.53% and 20.37% compared to T1 treatment. In 2021,T5 treatment had the highest sugar beet yields of 67 934 and 70 863 kg/hm²,respectively,increased by 14.55% and 12.54% compared to T1 treatment. In 2020,T3 treatment had the highest the sugar yields of 11 737 and 12 314 kg/hm² in flat cropping and ridge cropping respectively,increased by 30.93% and 24.68% compared to T1 treatment. In 2021,T5 treatment had the highest sugar yields of 10 836 and 11 374 kg/hm² respectively,increased by 17.50% and 13.06% compared to T1 treatment. Under the flat cropping and ridge cropping modes,the models of sugar beet yield and microbial fertilizer application rate were y=-0.224 6x²+96.845x+58 126,R²=0.817**;y=-0.207 8x²+88.685x+62 334,R²=0.723**. The models of sugar yield and microbial fertilizer application rate were y=-0.049 8x²+20.112x+9 036.4,R²=0.806**;y=-0.045x²+18.215x+9 929,R²=0.714**. Based on the above models,it was calculated that the maximum yields of 68 568 and 71 796 kg/hm² can be achieved by applying microbial fertilizer of 215.6 and 213.4 kg/hm² in flat cropping and ridge cropping mode. The maximum sugar yield of 11 067 and 11 772 kg/hm² could be achieved when microbial fertilizer was applied at 201.9 and 202.4 kg/hm² respectively. The model tests showed that the linear relationship between the measured and estimated sugar beet yields,as well as the measured and estimated sugar yields,all had extremely significant correlations,indicating good accuracy of the models.【Conclusion】The application of microbial fertilizer in saline-alkali land of the Yellow River Irrigation Area significantly improved the yield and quality of sugar beet. The optimal application rates of microbial fertilizer were 201.9-215.6 and 202.4-213.4 kg/hm² in flat cropping and ridge cropping. [ABSTRACT FROM AUTHOR]

Preventive environmental public interest litigation contains the inherent mechanism of risk prevention, which can help improve the regulatory system against environmental risks and respond to the public’s demand for preventing environmental risks. Currently, although China has adopted the expression of ‘significant risk’ at the legislative level, legislators have not provided relatively comprehensive and specific explanations on its inherent nature, specific judicial identification methods, and proof standards for specific causal relationships. How to correctly identify and confirm ‘significant risks that harm public interests’ is left to the discretion of the courts: either by uniformly recognizing administrative actions as references, directly adopting risk assessment results from scientific and technological evaluations, or independently evaluating significant risks. It is also unclear whether the term applies to environmental pollution or ecological destruction. Fundamentally, due to a lack of a unified understanding of ‘significant risks,’ judges mostly make judgments based on environmental risk assessment reports, which cannot properly handle the relationship between preventive environmental judicial powers and environmental administrative powers, resulting in judicial discretion becoming formalistic. Fundamentally, the judicial determination of significant risks faces limitations such as inconsistent connotations, misplaced recognition subjects and unclear standards, and rigid confidence mechanisms, making it difficult for lawsuits to correctly handle the dialectical relationship between judicial initiative and restraint. To solve the confusion caused by unclear concepts and mechanical judgments and to properly give play to the judiciary’s initiative, this article responds to practical problems in three ways: first, defining the connotations of ‘significant risks,’ clarifying their concepts, and confirming their basic objects by taking a literal decomposition and responsibility backtracking approach; second, defining the identification criteria for significant risks, using dynamic adjustments under risk assessment uncertainty as the premise for defining identification criteria and using the high probability of serious harm consequences as the causal requirement for identification criteria; and third, optimizing the confidence mechanism of significant risks, using the organic combination of objective scientific rationality and subjective social rationality as the inherent logic of the confidence mechanism and adopting a phased confidence mechanism arrangement for two different levels of significant risk identification. [ABSTRACT FROM AUTHOR]

Objective To analyze the effect of subclinical hypothyroidism(SCH)on endocrine and metabolic characteristics of polycystic ovary syndrome(PCOS)patients, and to explore the correlation between thyroid stimulating hormone (TSH) level and various endocrine and metabolic indexes. Methods Totally 661 patients with PCOS in the outpatient Department of Endocrinology and Metabolism of the First People's Hospital of Yunnan Province from January 2020 to June 2021 were selected as the research object. The general data and related endocrine and metabolic indexes of the patients were collected. Taking the TSH value of 4.2 mIU/L as the cut-off point for the diagnosis of SCH, PCOS patients were divided into SCH group and non SCH group. The differences of endocrine and metabolic characteristics between the two groups were compared; The correlation between TSH level and its endocrine and metabolic indexes was analyzed. Results The incidence of SCH in PCOS patients was 31.16%. Compared with PCOS patients without SCH, the levels of TSH, prolactin and triglyceride in the group with SCH were significantly higher, and the levels of free thyroxin and estradiol were significantly lower (P＜0.05). TSH level was negatively correlated with free thyroxin in PCOS patients (r=-0.152, P＜0.001), and positively correlated with total cholesterol and triglycerides (r=0.133, 0.111, P＜0.01,P＜0.05, respectively). Conclusions PCOS patients with SCH have more serious endocrine hormone disorders and dyslipidemia, which may further aggravate the risk of cardiovascular diseases and reproductive abnormalities in PCOS patients. It is suggested that patients with PCOS should be screened for thyroid function and lipid metabolism regularly and followed up for a long time. [ABSTRACT FROM AUTHOR]

BACKGROUND: Tranexamic acid has been widely used in orthopedic surgery as a hemostatic drug. However, after arthroscopic surgery, the cartilage, tendons and ligaments are directly exposed to tranexamic acid. It is necessary to reiterate the importance of cartilage again. OBJECTIVE: To analyze the effect of topical tranexamic acid on cartilage through the existing experimental research and clinical data. METHODS: Related documents were searched in CNKI, WANFANG MED ONLINE, and PubMed by computer. The retrieval time was from the inception of the database to February 10, 2020. The Chinese and English key words were “tranexamic acid, arthroscopy, cartilage, toxicity”. RESULTS AND CONCLUSION: The dosage of clinical tranexamic acid is still based on the experience of physicians. There is great controversy regarding its safe administration concentration and exposure time. There is a lack of precise clinical guidelines. Based on the existing in vitro experiments, it was found that between 25-50 g/L, there should be a concentration that is safest for cartilage biological activity. Before there are further clinical evidences, it should be recommended that the local concentration of tranexamic acid should not exceed 25 g/L. When using tranexamic acid for washing, the mass concentration should not exceed 70 g/L. Regarding the most appropriate mass concentration of tranexamic acid for topical application, further research and clinical trials are still needed to better clarify its safety in topical use [ABSTRACT FROM AUTHOR]

The experiment was to determine the effect of seaweed fertilizer on the yield and nutritional value of alfalfa in returning green stage. The alfalfa cultivar Jinneng 5020 was used as experimental materials, it was sprayed seaweed fluid fertilizer P1 (1 g/m²) and P2 (2 g/m²) at a dilution of 650 times, and the alfalfa in control group was sprayed by water. The indicators were measured during the budding period. The results showed that the seaweed fertilizer treatments significantly improved the plant height, leaf weight, yield of alfalfa and SPAD value. Compared with control group, the CP content of P1 treatment was significantly decreased (P<0.05), the ADF content and NDF content were significantly increased (P<0.05). Compared with control group, CP content of P2 treatment was increased. Compared with control group, the seaweed fertilizer treatments decreased the ton milk index, but significantly increased the milk production per unit area of forage (P<0.05). The study indicates that it was suitable to apply 2 g/m² of seaweed fertilizer, 650 times dilution and spraying on the leaves of alfalfa in the Yellow River beach area during the green returning period, which can achieve the goal of increasing the alfalfa yield and planter income. [ABSTRACT FROM AUTHOR]

Objective: To study the characteristics of platelet rich plasma(PRP) in the treatment of lower limb skin defect and to find out the most suitable treatment for lower limb skin defect with no surgical indications. Methods: From 2016.03 to 2017.03, 16 patients diagnosed as lower limb skin defect were included in the research. 8 patients were treated by platelet rich plasma, while 8patients received conservative treatment with recombinant human epidermal growth factor gel. Remove residual hemostatic gauze and dried blood every and change the new drug 7 days and observe the healing of the wound. Results: We observed that the scabby appeared in the skin defect, and there was no leakage in the wound in seventh day in all patients. The area was reduced about 1/4 in study group,1/6 in group in 7 days in control group. The control group were observed after 1 week, 1 case with infection, give again complementary makings bandage after debridement and using antibiotics. The area was reduced about 2/3 in experimental group, 1/4 in group in 14 days in control group. 3 weeks after treatment, the experimental group patients have been healed by more than 85% of the wound. With delayed 1-2 weeks after treatment all of the patients of this group had healing. Two patients in control group patients in the treatment of three weeks had more than 50% healing area who were continued to use this treatment method. The remaining 6 patients underwent surgical treatment(VSD) + skin graft/flap whose area reduced about 1/5. Comparing to the control group, the area of group A and B was significantly reduced(P <0.05). The area of study group was significantly reduced than the control groups(P < 0.05). Conclusions: The therapy of PRP can promote wound healing, shorten hospitalization time, reduce laid-in-bed complications, reduce the economic burden in the treatment of lower limb skin defect. [ABSTRACT FROM AUTHOR]

Objective: The aim of this study was to evaluate the clinical effects in patients who had undergone primary total knee arthroplasty(TKA) with 2 different closed negative pressure drainage(not clip pipe; clip to remain open after 6 hours). Methods: A total of 52 patients(52 knees) underwent primary TKA in our hospital from 2015.06 to 2017.06 was conducted. They were randomly divided into two groups(don’t clip pipe group, clip pipe 6 h). Comparing three groups of patients with postoperative total flow rate and amount of allogeneic blood transfusion, total blood loss and hidden blood loss, postoperative week diameter growth of leg, incision infection rate,incidence of thrombosis and the function of knee joint after December(HSS score) evaluate the situation. Results: There were no significant difference in the baseline characteristics including age, gender, height, weight and time of operation between the two groups(P>0.05). However, total blood loss, hidden blood loss, blood transfusion in study group were higher than that in control group(P<0.05).Multiple comparison between groups of patients, postoperative 1 d, 3 d weeks diameter growth of leg, postoperative incidence of thrombosis, incision infection rate and postoperative HSS score 12 months were no significant statistical difference(P>0.05). Conclusion: Early continuous negative pressure drainage after clip pipe(6 h) can significantly reduce the amount of blood loss and the blood transfusion rate, and do not increase the obvious adverse consequences. [ABSTRACT FROM AUTHOR]

Objective: To investigate the clinical value and effect of spinal nerve medial branch ablation through transforaminal endoscopic in treatment of lumbar zygapophysial joint pain. Methods: From 2015.01 to 2017.01, 92 patients diagnosed as lumbar zygapophysial joint pain were included in the research. 36 patients were treated by spinal nerve medial branch ablation through transforaminal endoscopic, while 56 patients received conservative treatment. The visual analogue scale(VAS)and Japanese Orthopedics Association(JOA)scores before treatment, 1, 6 and 12 months after treatment were recorded and analyzed. The MacNab scores were recorded and analyzed 12 months after treatment. Results: The VAS and JOA scores of surgical treatment group showed significant improvement compared with that before operation(P<0.05), and there were no significant difference at each time point after surgery. The VAS and JOA scores of conservative treatment group in showed significant improved at 1 months after treatment compared with that before treatment(P<0.05), but there were no significant difference at each time after treatment compared with pretreatment. The VAS and JOA scores of surgical treatment group showed significant improvement compared with conservative treatment group at each time after treatment(P<0.05). The MacNab scores of surgical treatment group(94.44%)was significantly higher than that of conservative treatment group(33.33%). Conclusions: The spinal nerve branches ablation under transforaminal endoscopic is an effective and safe minimally invasive spine surgery technique with important clinical value in the treatment of lumbar zygapophysial joint pain. [ABSTRACT FROM AUTHOR]

Objective: To investigate the value of ultrasonic system screening in the diagnosis of congenital malformation of fetal central nervous system in early pregnancy. Methods: From March 2014 to April 2017. 20S4 cases of pregnant women with fetal nuchal translucency in early pregnancy in our hospital were enrolled, including 2135 fetuses. The ultrasonic measurement of fetal crown rump length and nuchal translucency values through the head sagittal section, lateral ventricle, cross-sectional level of cerebellum and posterior cranial fossa, spine section 4 long axis cross sectional anatomy of the standard ultrasound fetal central nervous system structure in system inspection, statistical analysis and display the value of XT in the 4 section of the standard ultrasonic examination, and the analysis of the NT value and the standard section display rate and the relationship between gestational age and early pregnancy by ultrasound examination of the standard section of central nervous system abnormalities detection rate, sensitivity, specificity, positive predictive value and negative predictive value of statistical analysis. Results: The fetus in early pregnancy by ultrasonography, ultrasound imaging showed head chest median sagittal and lateral ventricle level transverse, cerebellum and posterior cranial fossa, spine section long axis cross study shows that rates were 98.17%. 99.44%. 98.78% and 98.97%. different groups of gestational age between ultrasonic section display rate difference contrast, v2the test values were 0.25. 1.65. 0.62. 0.13. the difference was not statistically significant (P>0.05); early pregnancy ultrasound diagnosis of fetal malformation of the central nervous system's sensitivity was 90.91%. specificity was 100%. the positive predictive value was 100%. the negative predictive value was 99.48%: the XT value and the normal fetal gestational age was positively correlated (r=0.153). Conclusion: Ultrasonography has a higher display rate of fetal central nervous system structural malformations in early pregnancy, and is not affected by gestational age. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the clinical efficacy and safety of using buprenorphine transdermal patch in the treatment of patients with knee osteoarthritis. Methods: 112 cases of knee osteoarthritis were divided into 2 groups: the experimental group (n=56) was treated with a total of two patches of buprenorphine (5 mg) transdermal patch on the outside of upper arm, and each patch had been kept for 7 days. The control group (n=56) was given 20 mg celecoxib orally twice daily for two weeks. patients were standardized using analgesics according to the VAS score. McMaster Universities Osteoarthritis Index (WOMAC), and adverse reactions. Results: 112 cases of knee osteoarthritis patients were observed. The visual analog scale (VAS) reported by patients in the experimental group and the control group were significantly decreased after two weeks treatment (P<0.05). However, there was no significant difference between 2 groups. The WOMAC score in the experiment group and the control group were significantly decreased after the treatment (P<0.05). There was no significant difference between the two groups (P>0.05). There was no significant difference between 2 groups in complication and adverse reactions. Conclusions: Buprenorphine transdermal patches is at least as effective as celecoxib in treating knee osteoarthritis which could be further used in clinic therapy because of its easy using. [ABSTRACT FROM AUTHOR]

Objective: To introduce and evaluate a new clinical grouping and therapeutic schedule of heel pain caused by calcaneal spurs and plantar fasciitis. Methods: 45 patients (60 feet) who were admitted in our hospital from 2013.01 to 2015.01 and diagnosed as heel pain caused by calcaneal spurs and plantar fasciitis were admitted in our experiment. Results: 1)The relationship between the symptom, function and the new clinical grouping and the relationship between substance p and the new clinical grouping were expored. 2) 45 patients were brought into 3 groups randomly: A: spurs excision; B: substance p inhibition; C:(both). Clinical outcomes were evaluated by visual analogue scale (VAS) and the American Orthopedic Foot and Ankle Society (AOFAS) ankle-hindfoot scores. 1) There were significant difference in the VAS and AOFAS between the I patients and the II patients. However, no difference were found in the spur index. 2) There were significant difference in the substance p, VAS and the clinical grouping. 3) All patients were improved after the therapy in all 3 groups and the group C was significantly better than other groups. No other complication was shown. The new clinical grouping has significant advantage in diagnosis and therapy. Conclusion: The new therapy methord of spurs excision and substance p inhibition has notable dominance than the normal methord, which could be a new and effectivly better way to cure heel pain. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical effect and safety of different depressing segments（responsible segment or total segments） in the treatment of herniation of Multi-segmented lumber intervertebral disc, HMLD. Methods: 78 cases of patients with herniation of Multi-segmented lumber intervertebral disc treated from 2010.01 to 2013.01 were retrospectively reviewed, in which 42 patients were treated by only responsible segment and 36 patients were treated by total segments. The clinical outcomes were evaluated and compared by lost, operation time, blood loss, postoperative hospital stay and intraoperative complications. The surgical results were evaluated according to the Visual Analogue Scale(VAS) and Japanese Orthopedic Association(JOA). Results: There was no significant difference between the two groups(experiment group and total segments) in the VAS score and JOA in 6, 12 and 36 months after the operation(P>0. 05). However, the VAS was significant better in experiment group on the day after the operation(P<0.05). The blood lost, money lost and the stay time in experiment group were significant better than that in control group (P<0.05); The complication incidence in experiment group was significant better than that in control group in the first year after the operation. One yaras later, the complication incidence in control group was less, however, there was no significant difference between the two groups (P>0.05). Conclusion: For the treatment of herniation of Multi-segmented lumber intervertebral disc, finding the responsible segment and treating could have the similar clinical efficacy compairing with the total segments depressing, which could have the less lost,operation trauma and the short-term complication. This treatment could be further recommended clinically. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical effect and safety of different depressing segments（responsible segment or total segments） in the treatment of herniation of Multi-segmented lumber intervertebral disc, HMLD. Methods: 78 cases of patients with herniation of Multi-segmented lumber intervertebral disc treated from 2010.01 to 2013.01 were retrospectively reviewed, in which 42 patients were treated by only responsible segment and 36 patients were treated by total segments. The clinical outcomes were evaluated and compared by lost, operation time, blood loss, postoperative hospital stay and intraoperative complications. The surgical results were evaluated according to the Visual Analogue Scale(VAS) and Japanese Orthopedic Association(JOA). Results: There was no significant difference between the two groups(experiment group and total segments) in the VAS score and JOA in 6, 12 and 36 months after the operation(P>0. 05). However, the VAS was significant better in experiment group on the day after the operation(P<0.05). The blood lost, money lost and the stay time in experiment group were significant better than that in control group (P<0.05); The complication incidence in experiment group was significant better than that in control group in the first year after the operation. One yaras later, the complication incidence in control group was less, however, there was no significant difference between the two groups (P>0.05). Conclusions: For the treatment of herniation of Multi-segmented lumber intervertebral disc, finding the responsible segment and treating could have the similar clinical efficacy compairing with the total segments depressing, which could have the less lost,operation trauma and the short-term complication. This treatment could be further recommended clinically. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical effect and safety of percutaneous pedicle fixation or open operation in the treatment of multiple-level thoracolumbar fracture. Methods: 56 cases of patients with multiple-level thoracolumbar fracture treated from 2013.06 to 2015.06 were retrospectively reviewed, in which 32 patients were treated by percutaneous pedicle fixation and 24 patients were treated by open operation of pedicle fixation. The clinical outcomes were evaluated and compared by operation time, blood loss, stay time and intraoperative complications. The surgical results were evaluated according to the Visual Analogue Scale(VAS), Japanese Orthopedie association( JOA) and anterior vertebra height. Results: There was no significant difference between the two groups(experiment group and control group) in the VAS score and JOA in 6 and 12 months after the operation (P>0.05) although there were significant improvement comparing with pre-operation (P<0.05). The anterior vertebra height was better in open operation group, however, there was no significant difference between the two groups (P>0.05). The blood lost, operation time and the stay time in experiment group were significant better than that in control group. (P<0.05); The complication incidence in experiment group was significant better than that in control group (P<0.05). Conclusions: For the treatment of multiple-level thoracolumbar fracture, percutaneous pedicle fixation could have the similar clinical efficacy comparing with the open operation, which could have the less blood loss, operation time, stay time and the complication. This treatment could be further recommended clinically. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical effect and safety of patients with lumbar disc herniation treated with microendoscopic discectomy (MD) and percutaneous endoscopic lumbar discectomy (PELD). Methods: We retrospectively reviewed 118 patients with lumbar disc herniation treated from 2012.06 to 2015.06. 46 patients received MD and 72 patients were given PELD. All patients received at least 6 months fellow up. Clinical outcomes were evaluated by operation time, the time in bed, blood loss, the length of incision, cost, lumbar vertebral function and complications and so on. Results: The VAS score and lumbar function of the patients were significantly better than pre-operation, but there was no difference between two groups. The time of operation and the time in bed and the cost of patients who had PELD was significantly better compared with those who had MD (P<0.05). There was no difference between two groups in the time in hospital, the blood loss, length of incision and the complication. Conclusions:MD and PELD as the micro-spine operation have obvious advantages and good safty in the treatment of lumbar disc herniation. They have the advantages and disadvantages respectively. It should be selected personality in clinic. [ABSTRACT FROM AUTHOR]

Objective: miRNA-194 were knocked down or overexpressed in Osteosarcoma Cell Line SOSP-9607 to investigate its effect on Metastasis of human Osteosarcoma which to provide the foundation of theory of miRNA-194 as a new target of osteosarcoma therapy. Methods: We transfected human osteosarcoma cell lines SOSP-9607 by using recombinant lentiviruses and divided the cells into three groups with different levels of miRNA-194. Then the stable transfected cells were used in transwell and wound healing assay. Results:1) The transfection and selection of miRNA-194 using recombinant lentiviral expression vector was finished correctly and different levels of miRNA-194 were got sucessfully. 2) Results in the transwell migration and invasion assay showed significantly lower numbers of OE SOSP-9607 cells (28.60± 4.36) and (21.25± 6.42) and significantly higher numbers of KD SOSP-9607 cells (132.60± 15.64; 115.76± 11.38) compared with other groups (p<0.05). The wound healing assay showed that cells in OE groups exhibited a significant decrease in migration rate compared to the other three groups(p<0.05). Conclusions: miRNA-194 could inhibit metastasis of Osteosarcoma Cell Line SOSP-9607. MiRNA-194 could be further explored as a potential target in Osteosarcoma diagnosis and therapy. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the clinical outcomes of percutaneous vertebroplasty(PVP) in treatment of vertebral hemangioma. Methods: The clinical data of 46 patients who were diagnosed as vertebral hemangioma and treated with PVP in our hospital from2008 to 2015 were retrospectively analyzed. All patients were followed up at least 6 months after the operation. The clinical outcomes,recurrence, curative effect, life quality and safety were evaluated and compared by visual analogue scale(VAS), modified Macnab criteria and SF-36 before and after operation. Results: The results of postoperative VAS score, quality of life were obviously improved compared with preoperative evaluation(P<0.05), and VAS score was gradually decreased with the time after surgery. There were 2 cases recurrence which had no symptom and no treatment. No other complications were detected. According to the modified Macnab criteria, the outcome showed the excellent and good rate was 93.5 %. Conclusions: PVP is a safe, effective and minimally invasive treatment for the vertebral hemangioma, which can obviously relieve the pain symptoms, and significantly improve the lumbar vertebral function and quality of life. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical effect and safety of patients with lumbar disc herniation treated with percutaneous endoscopic lumbar discectomy(percutaneous endoscopic lumbar discectomy, PELD) between vertebral plate or limited electric drill discectomy. Methods: We retrospectively reviewed 126 patients with lumbar disc herniation treated from 2012 to 2014. 48 patients received percutaneous endoscopic lumbar discectomy between vertebral plate and 78 patients were given limited electric drill discectomy(control group). Clinical outcomes were evaluated by cost, lumbar vertebral function and complications and so on. Results: The VAS score, ODI, and lumbar function of the patients were significantly better than pre-operation, but there was no difference between two groups. The time in hospital, blood loss and length of incision of patients who had PELD was significantly better compared with those who had limited electric drill discectomy(P<0.05). Conclusions: PELD between vertebral plate as one minimally invasive surgery has obvious advantages in some aspects of the treatment of lumbar disc herniation, which could be a good supplementary for PELD in clinic. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical effect and safety of patients with giant cell tumor in distal radius treated with different operations. Methods: 88 patients with giant cell tumor in distal radius who had surgical indications were involved. 3 major operations were used in the treatment according to the Campanacci grading system. Group A were treated with hyperthermia by inserted microwave antennas after tumor exposure in situ and the resulted bone defect were reconstructed with the compound including self-ilium,allogeneic bone powders and bone cement. Patients in group B underwent en bloc resection of bone tumor with autologous fibula.Patients in group C underwent curettage, adjuvant phenol therapy, and bone grafting. Clinical outcomes were evaluated by Enneking, and the cost, recurrence was compared between three groups. Results: The tumor recurred in 5 patients in group A with a recurrence rate of10.87 %. According to the Enneking functional evaluation system, MSTS93 score was 26.32 ±2.92. None people in B group had the recurrence and the MSTS93 score was 22.85±4.16. The recurrence rate of group C was 33.33 % and MSTS93 score was 22.97±3.84.There were significant statistical differences in recurrence rate between 3 groups(P<0.05). The functional score in A and C are significantly better than that in B group, while there was no significant statistical difference between A and C(P>0.05). There was 1superficial infection in A group and 2 cases in B group, which were cured dressing. Conclusions: En bloc resection of bone tumor could reduce the recurrence rate while it had the poor function recovery and more risks of complications. Microwave-induced hyperthermia could kill the tumors completely and had better function recovery, but it had the great recurrence. The schedule of surgery should be done according to patients’ condition. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical effect and safety of patients with vertebral hemangioma treated with percutaneous vertebroplasty (PVP) or Percutaneous kyphoplasty (PKP). Methods: 48 patients with vertebral hemangioma who had surgical indications were randomly divided into the PVP group (27 cases) and PKP group (21 cases) , in which the patients were treated with PVP and PKP, respectively. Clinical outcomes were evaluated by visual analogue scale (VAS), the cost, the pain during the operation and complications like bone cement between two groups. Results: The VAS score of PKP group was lower than PVP, however, which had no significantly differences (P>0.05). There were significantly differences on the total cost, the pain during the operation and complications (P<0.05). Conclusions: There are no significantly difference between PVP and PKP on visual analogue scale after operation. Howerer, PKP has obvious advantages in some aspects of the treatment of vertebral hemangioma including the feeling in surgery and safety. Treatment choice should be done according to patients' condition. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the clinical efficacy of modified McBride orthomorphia with small incision in the treatment of Hallusx Valgus. Methods: 104 patients who were admitted in our hospital from 2010 to 2014 and diagnosed as Hallusx Valgus were cured by modified mcBride orthomorphia. The clinical efficacy was evaluated by visual analogue scale (VAS), the changes of hallus valgus angle(HVA) and intermetatarsal angle(IMA) and Ankle Society score(AOFAS) at reoperation, 3 weeks after surgery and 6 weeks after surgery. Results: All the patients were followed up at least 6 months after the operation and the excellent rate was 96.2%. The postoperative VAS score, hallus valgus angle and Ankle Society score at 3 weeks after surgery were obviously improved compared with those preoperation (P<0.05). Only three patients had recurrence at 6 months after surgery, 6 patients had mild pain of foot and 1 case had delayed healing of incision. Conclusions: Modified McBride orthomorphia with small incision could effectivly correct the foot hallux valgus deformity and relieve the pain due to this disease. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the clinical efficacy of cocktail neuroprotective agents(N6) in treatment of spasm in patient with traumatic spinal cord injury (TSCI). METHODS: 189 patients with TSCI diagnosed by Jinan Military General Hospital from Jan.2013 to Jun.2016 were retrospectively selected and divided into observation group (102 cases) and control group(87 cases) according to the application of cocktail neuroprotective agents(N6). The incidence of spasm of two groups were observed and compared at 2 months and 12 months after injured;for patients stricken by spasm at 2 months after injured, the total effective rate at 12 months after injured were observed and compared. RESULTS: At 2 months and 12 months after injured, the incidence of spasm of the observation group was significantly lower than that of the control group, with statistically significant difference (P ＜ 0.05). 12 months after injured,the total effective rate of the observation group (62.79％, 27/43) was significantly higher than that of the control group(35.85％, 19/53), with statistically significant difference(P ＜0.05). CONCLUSIONS: The cocktail neuroprotective agents(N6) can effectively reduce the incidence of spasm in patient with TSCI, with high cure rate. [ABSTRACT FROM AUTHOR]

Objective: To compare the clinical effect and safety of Percutaneous Endoscopic Lumbar Discectomy(PELD) with limited electric drill discectomy in the treatment of patients with lumbar disc herniation. Methods: 192 cases of patients with lumbar disc herniation treated from 2011 to 2014 were retrospectively reviewed, in which 118 patients were treated by PELD and 74 patients were treated by limited electric drill discectomy(control). The clinical outcomes were evaluated and compared by visual analogue scale(VAS),Lehmann Lumbar function score, modified Macnab criteria, routine operation indicators and incidence of complications between two groups. Results: The VAS score, lumbar function and total cost of patients who were treated by PELD was similar to that of patients treated by limited electric drill discectomy(P>0.05). The time in hospital, intraoperative blood loss and incidence of complications were shorter or lower in PELD group compared with the cobtrol group(P<0.05). Conclusions: PELD had obvious advantages of some aspects in the treatment of lumbar disc herniation, which could be further carried out in clinic. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the clinical efficacy of percutaneous transforaminal endoscopic discectomy(PTED) in the treatment of lumbar disc herniation. Methods: The clinical and follow-up data of 194 patients who were admitted in our hospital from 2009 to 2013 and diagnosed as lumbar disc herniation were treated by PTED were retrospectively analyzed. The clinical efficacy was evaluated by visual analogue scale(VAS), Lehmann Lumbar function score, modified Macnab criteria and SF-36 before and after operation. Results: All the patients were followed up at least 12 months after the operation. The postoperative VAS score, lumbar function, quality of life were obviously improved on the day after surgery, at the 3rd, 12 th month after surgery compared with those preoperation(P<0.05)..At the 3rd, 12 th month after surgery, the excellent rate of modified Macnab criteria were 90.7%, 92.3%,respectively. Conclusion: PTED was a safe and effective treatment for lumbar disc herniation, which could obviously relieve the lumbar spinal function and improve the quality of life with high safety. [ABSTRACT FROM AUTHOR]

Objective: The ultra-small superparamagnetic iron oxide particles were used as the carrier to construct and characterize nuclear magnetic/fluorescent molecular probes through polyethylene glycol-linked fluorescent Cy5.5. Methods: Ake a small amount of Cy5.5-nhs fluorescent powder and dissolve it in dimethyl sulfoxide （DMSO） solution. After centrifugation and ultrafiltration of PEG ferric oxide particles, resuspend the nanoparticles with phosphate buffered saline （PBS） to change the pH of PEG ferric oxide nanoparticle solution. The configured cy5.5 fluorescence was added to Fe3O4particles, incubated in a constant temperature shaking table, and the larger iron ions and unbound fluorescence were removed through a centrifugal filter. After standing, the hydrated particle size and zeta potential were detected, the relaxation rate was detected by newwheat nuclear magnetic resonance, the cytotoxicity was detected by CCK-8 experiment, and the uptake of the probe by cells was observed by laser confocal microscope. Results: The Cy5.5-PEG-Fe3O4probe was successfully synthesized. Transmission electron microscope （TEM） showed that the diameter of the probe was 16.8 ±2.4nm,the water and diameter of the nanoparticles were 43.4±17.6 nm, and the Zeta potential was -18.0 m V. The relaxation rate is 39.5 m M-1·s-1and the slope is 0.98. The results of cytotoxicity experiments show that it is slightly toxic to cells, and the toxicity is dependent on the concentration. Laser confocal results show that this probe can be taken up by cells smoothly. Conclusion: This experiment successfully synthesized Cy5.5-PEG-Fe3O4probe. [ABSTRACT FROM AUTHOR]

Objective The aim of this study was to discuss and develop the most sensitive non operative method for anterior cruciate ligament rupture. Retrospective analysis was performed on patients who underwent arthroscopic knee arthroscopy for anterior cruciate ligament rupture. Before operation, the positive rate of ligament rupture was tested by using anterior drawer test (ADT), Lanchman test and nuclear magnetic resonance imaging (MRI) examination. Methods: Collect the clinical data of the patients with anterior cruciate ligament injury diagnosed in department of orthopedics in our hospital during the past 10 years. Analysis ADT, Lanchman test and MRI before operation, then compare the statistical difference of the positive rate and sensitivity and specificity of each correlation. Results: 210 cases of arthroscopic surgery were collected in this research. The positive rates of diagnosis of preoperative examinations were as follows: ADT: 61.4 %, Lanchman: 69.5 %, MRI: 87.1 %. Sensitivity test are as follows: ADT: 61.5 %, Lanchman: 69.5 %, MRI: 91.4 %. Test specific are as follows: ADT: 60.9 %, Lanchman: 69.6 %, MRI: 52.2 %. Conclusions: ADT, Lanchman test, MRI for the diagnosis of anterior cruciate ligament rupture are deviation. In order to get the most accurate diagnosis of anterior cruciate ligament rupture, the clinical diagnosis and treatment process should be combined with all kinds of test results. [ABSTRACT FROM AUTHOR]

The thermal conductivity of cultivation substrates has been one of the most important parameters in the heat transmission of ground under the thermal environment in a solar greenhouse. Among them, the cultivation substrate is one type of multicomponent material with solid and liquid phases. In this study, prediction models were proposed for the effective thermal conductivity of unsaturated cultivation substrates in the production of a solar greenhouse. Eight commonly-used single substrates were taken as the research objects, such as perlite, vermiculite, cinder, river sand, coco coir, peat, decomposed cow dung, and peanut shell. The thermal conductivities of the substrates were measured using the guarded hot plate apparatus. The test temperature was set at 20 ℃, where the temperatures of the hot and cold plates were 25 and 15 ℃, respectively. Firstly, the effective thermal conductivity of the solid phase in the eight single substrates in dry and saturation was determined to reversely calculate the hybrid models for the effective characteristics of composite materials, such as Series, Parallel, Johansen Geometric Mean, Maxwell-Eucken 1, Maxwell-Eucken 2, Effective Medium Theory, Series Parallel-Arithmetic Mean, Series Parallel-Harmonic Mean, Series Parallel-Geometric Mean, Maxwell Eucken-Arithmetic Mean, Maxwell Eucken-Harmonic Mean, and Maxwell Eucken-Geometric Mean model. The solid-phase thermal conductivities of perlite, vermiculite, cinder, river sand, coco coir, peat, and decomposed cow dung and peanut shell were 0.058, 0.139, 0.252, 0.817, 0.148, 0.518, 0.262, and 0.066 W/ (m·K), respectively. Secondly, the components of the solid phase were arranged in parallel using the forward and reverse calculation of the hybrid model for the effective characteristics of the composite materials, according to the measured effective thermal conductivity of the complex substrates. The least-square method was selected to verify the thermal conductivity of the six single models. It was found that the Parallel model presented the smallest among the six single models, such as Series, Parallel, Johansen Geometric Mean, Maxwell-Eucken 1, Maxwell-Eucken 2, and Effective Medium Theory model. Finally, the correlation analysis was made between the thermal conductivity of the solid phase and the volume proportion of each component in the composite substrates. Furthermore, a comparison was made on the experimental and theoretical values for the effective thermal conductivity of the composite substrates under different saturation degrees. The results show that the calculated values of the Parallel model were the closest to the measured ones, and the least-squares calculation presented the smallest among the six prediction models of effective thermal conductivity. Therefore, the Parallel model was suitable for the theoretical calculation and prediction for the effective thermal conductivity of the composite substrates. Moreover, four seedling and cultivation substrates were selected to verify the model, such as vermiculite:peat=8:2, cow dung:perlite:coco coir=1:1:1, peanut shell:vermiculite:perlite=5:3:2, and river sand:peat:vermiculite=2:1:3, commonly-used in practice under different saturation degrees. The results show that the mean absolute percentage error of the Parallel model was 0.026 2%～0.137 4%, the root mean square error of the parallel model was 0.008 6～0.031 5 W/(m·K), the model determination coefficient R2 of the Parallel model was 0.900 7～0.988 0, and the prediction accuracy of the Parallel model was high. indicating better performance. Consequently, the Parallel model can be used to accurately predict the effective thermal conductivity of the cultivation substrates in actual production under various saturation degrees in a solar greenhouse. This finding can provide a strong reference for the effective thermal conductivity of porous media in unsaturated cultivation substrates of a solar greenhouse. [ABSTRACT FROM AUTHOR]

Objective: To study the difference between bone pouch vertebroplasty and percutaneous balloon dilatation vertebroplasty in the treatment of osteoporotic fracture of spine. Methods: From 2018.01 to 2021.01 95 cases of with osteoporotic fracture of spine were divided into two groups according to different operation ways. 41 patients underwent surgery using bone pouch vertebroplasty (mesh bag group), while 54 patients underwent surgery with conventional balloon dilatation vertebroplasty(PKP group). The VAS and ODI scores in 3days, 3 months after operations were analyzed. Standard thoracolumbar radiography were performed before and after operation. The imaging was compared before and after operation, including anterior edge height, posterior edge height, midline height and cobb angle. The bone cement distribution and the rate of bone cement leakage were evaluated of both groups. Results: 3 days and 3 months after operation, the VAS and the ODI were significant better (P<0.05), while there was no significant difference between 2 groups (P>0.05). In sagittal position, the anterior edge height, posterior edge height, midline height and cobb angle of the two groups recovered significantly compared with those before operation, but there was no significant difference between the two groups (P>0.05). The bone cement distribution and cement leakage rate was significantly better in the mesh bag group (P< 0.05). There was no significant difference in the complications between the two groups. Conclusion: Comparing the conventional PKP method, using the bone pouch vertebroplasty can effective improve bilateral distribution rate of bone cement, and reduce leakage rate of bone cement, which could be further promoted in clinic in osteoporotic vertebral compression fractures. [ABSTRACT FROM AUTHOR]

Objective: To forecast and verify the target genes of miRNA-194 in human osteosarcoma and establish some theoretical basis for osteosarcoma treatment and element experience of miRNA-194 for further research. Methods: Potential target gene of miRNA-194 was predicted using bioinformatics and were verified in gene and protein levels. Moreover, luciferase reporter assay was used to confirm whether CDH2 was direct targets of miRNA-194. Results: Potential target genes of miRNA-194 were predicted by using bioinformatics. Moreover, luciferase activity of the wild-type PTEN-3'-UTR reporter was significantly suppressed in miRNA-194up-regulation group compared with other groups in SOSP-9607 cells(P<0.01) while down-regulation group could significantly increase it(P<0.01). At the same time, western blot analysis revealed that miRNA-194 expression could affect the expression of CDH2 protein while there was no significant difference on m RNA level(P<0.05). Conclusion: CDH2, one target gene of miRNA-194, was predicted and verified, which provided the theoretical basis for the next study of miRNA-194 in the osteosarcoma. [ABSTRACT FROM AUTHOR]

Objective: To explore and describe the relationship of miRNA-192 function and clinicopathological features in human osteosarcoma and analyze the relationship between miRNA-192 and mortality, which support some theoretical basis for osteosarcoma treatment and element experience for further research. Methods: Real-time quantitative PCR analysis were used in 50 clinical osteosarcoma samples which were followed up. Then the relationship of miRNA-192 function and clinicopathological features(like sex,age) in human osteosarcoma were explored. Results: We found that there were statistically significant relationships between miRNA-192 expression and some clinicopathological features of osteosarcoma, including age(P = 0.028), clinical stage(P = 0.007), distant metastasis(P = 0.013) and patient mortality(P=0.001). Conclusion: In clinical specimens, miRNA-192 expression levels were correlated with some clinicopathological features of osteosarcoma, including age, clinical stage, distant metastasis and patient mortality, which provided the theoretical basis for the next study especially the biological therapy of miRNA-192 in the osteosarcoma. [ABSTRACT FROM AUTHOR]

Objective: We examined the effects of miRNA-194 on the capacities of tumor formation and pulmonary metastasis of osteosarcoma in nude mice by using recombinant lentiviruses. All these wouLd provide the foundation of theory to explore microRNA- 194 as a new target of osteosarcoma therapy. Methods: we transfected human osteosarcoma cell lines U2-OS by using recombinant lentiviruses and divided the mice into three groups. Then we put the cells into the mice to built the metastasis model of rude mice. 5 weeks later the mice were killed to compare the size and weight of tumor in leg and lung. Results: 1) The size and weight of miRNA- 194-down were obviously higher than that in other groups (P<0.05). 2) The lung of rude mice of miRNA-194-up cell were obviously better than that in other groups(P<0.05). 3) It were certified that the tumor were osteosarcoma and metastatic tumor. Conclusion: In vivo, miRNA-194 couLd significantly inhibit the capacities of tumor formation and pulmonary metastasis which declare that miRNA-194 may play the role of tumor suppressor genes in osteosarcoma, which provide a good foundation for the study of miRNA-194. [ABSTRACT FROM AUTHOR]

Objective: To investigate the safety and efficacy of the ultrasonic bone curette in the operation of thoracic ossification of ligamentum flavum, and the advantages of technology than the traditional high-speed drill. Methods: From 2016.01 to 2019.01，38 cases of thoracic ossification of ligamentum flavum were divided into two groups according to operation method. 17 patients received treatment with ultrasonic bone curette and 3D printing and digital technique, while 21 patients underwent surgery by high-speed drill. The lamina excision time, blood loss, the hospital length of stay (LOS) and the rate of complications such as dural injury and cerebrospinal fluid leakage were noted, and postoperative JOA score and the rate of postoperative neurological improvement were followed. Results: There was no significant difference between 2 groups on sex, age and average vertebral segment (P>0.05). The patients treated with ultrasonic bone curette and 3D printing and digital technique had better results in operation time and blood lose (P<0.05). The JOA score of two groups were significant improved after operation and there were significant difference between 2 groups (P<0.05). There were 4 cases of dural injury or cerebrospinal fluid leakage in observe group and 8 in control group. There was no significant difference between 2 groups (P>0.05). Among, 0 cases and 2 cases were found of equipment damage in observe group and control group respectively. Conclusion: Comparing the high-speed drill in decompression., using the ultrasonic bone curette and 3D printing and digital technique can significantly reduced the operation time and the bleeding amount while improve the clinic effect and no add the risk. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the clinical efficacy of ROI-C in ACDF and to explore its advantages and disadvantages. Methods: From 2017.06 to 2018.06, 84 cases of patients with Cervical spondylotic myelopathy included in this study, and all of the patients have received Anterior Cervical Discectomy and Fusion were divided into two groups according to different operation ways. 48 patients underwent surgery using ROI-C (experimental group), while 36 patients underwent surgery with Anterior Cervical Locking Plate System (control group). The operation time, bleeding and hospital stay were compared between the two groups, and using VAS score and JOA score to evaluate the clinical efficacy, and make a record of surgical complications, and SPSS 19.0 is used for statistical analysis. Results: There was no significant difference in VAS, JOA and Macnab score between the two groups in 3 days, 6 months and 12 months after operation (P > 0.05) while it has a statistically significant difference compared with preoperative (P < 0.01). Surgeries in both of the two groups were successfully completed, and there were no complications such as nerve injury, hematoma and infection (P > 0.05). There was significant difference in the operation time, operation blood lose and hospital stay time between the two groups. Conclusion: Comparing the traditional system of anterior cervical locking plate in ACDF, using the ROI-C in the treatment of cervical spondylosis has a definite clinical curative effect, however, which can short operation time and the reducing postoperative swallowing discomfort and short hospital stay time, which is worth to promotion and application. [ABSTRACT FROM AUTHOR]

Objective: To compare the 2 different ways of the effect of Kirschner wire tension band and hollow nail tension band in the treatment of transverse patellar fracture. Methods: 72 patients with patella fracture treated from June 2016 to October 2017 were selected as objects and randomly divided into control group(n=36) and observation group(n=36) according to the procedures of hospitalization. The control group was treated with Kirschner wire tension band internal fixation, and the observation group was treated with treated with hollow nail tension band fixation. Results: Compared to the two groups, the fracture healing time, postoperative complications, maximal knee flexion angle and Bostman patella score in observation group were better than those in control group(P<0.05). Conclusions: Compared with Kirschner wire tension band, hollow nail tension band has the advantages of simple operation, reliable fixation, quick fracture healing, good knee joint function and fewer postoperative complications. It is worthy of clinical application. [ABSTRACT FROM AUTHOR]

Objective: To investigate the clinical effect and safety of different blood purifications on the patients with multiple organ dysfunction syndrome caused by poisoning. Methods: From September 2015 to September 2018, 41 patients diagnosed as multiple organ dysfunction syndrome caused by poisoning were retrospectively analyzed. All the patients were divided into two groups by different blood purifications: experimental group (sequential blood purification group) or control group (single blood purification group). The clinical outcomes were evaluated by general situation (hospitalization time and hospitalization costs) and main blood indicators, APACHE Ⅱ score and survival rates after treatment. Results: Compared with the control group, the ALT, AST, CK-MB, LDH, Cr, Bun levels and APACHE Ⅱ score of treatment group were significantly lower than those of the control group after 5 days of treatment (P< 0.05). The hospitalization cost of treatment group was higher than taht of the control group(P<0.05), while the length of hospital stay and the mortality of treatment group showed no significant difference between two groups (P>0.05). Conclusion: Compared with the single mode of blood purification, sequential therapy can significantly improve the hepatic function, reduce the APACHE II score for poisoning MODS, though it is more expensive, it won't increase the hospitalization time. [ABSTRACT FROM AUTHOR]

Objective: To investigate the reliability of using the template with guide rods for atlantoaxial pedicle screw placement, and to compare the clinical outcomes of atlantoaxial pedicle screw placement by using the novel 3D printed template and by the conventional method. Methods: From 2017.09 to 2018.09，58 patients diagnosed as cervical spondylotic disease were retrospectively analyzed. All the patients were divided into two groups by different surgery types. 28 patients underwent surgery using a novel 3D printed drill guide template (experimental group), while 30 patients underwent surgery by conventional method (control group). The clinical outcomes were evaluated by operation time, intraoperative blood loss, frequency of fluoroscopy in surgery, screw placement accuracy. Japanese Orthopaedic Association (JOA) score of cervical neurological function, and visual analogue scal (VAS) of pain were used for comparisons between the two groups. Results: All the patients underwent successful 6 months completed follow-up. The experimental group had similar results in blood loss, pre-and postoperative JOA scores and VAS scores, and improvement rate of JOA score with those in control group(P>0.05). The intraoperative fluoroscopy frequency and operation time was significantly lower in the experimental group (P< 0.05), and the screw placement accuracy was significantly higher in the experimental group than those in control group (P< 0.05). Conclusions: Comparing the conventional method, using the novel drill guide template can result in lower intraoperative fluoroscopy frequency and higher screw placement accuracy without increasing the risk, which could be further promoted in clinic. [ABSTRACT FROM AUTHOR]

Objective: To explore the clinical effect and safety of percutaneous endoscopic lumbar discectomy(PELD) using Proxomed Tergumed in the treatment of patients with lumbar disc herniation. Methods: From June 2016 to June 2017, 63 cases of patients who were diagnosed as lumbar disc herniation and operated in our hospital were retrospectively analyzed. All the patients were divided into the experimental group(Proxomed Tergumed group) and the control group(tradition group). The clinical outcomes were evaluated by visual analogue scale(VAS) and Oswestry Disability Index(ODI) score, muscular power and complications at 1 week, 3 months and 6 months after operation. Results: The VAS score and ODI score of both groups were significantly lower than those preoperation. There was no obvious difference in the VAS and ODI score at the the first week after operation between two groups(P>0.05), while the VAS and ODI core of experimental group at 3 and 6 months after operation were significantly lower than those of the control group(P<0.05). Compared with the preoperative muscular power, there was statistical differences at 6 months after operation(P<0.05). There was no obvious difference in each muscle strength at 6 months after operation in the experimental group(P>0.05), which were significantly lower in control group(P<0.05). Conclusions: As a part of early rehabilitation training for patients who have undergone treated with percutaneous endoscopic lumbar discectomy, Proxomed Tergumed can effectively reduce the possibility of postoperative complications, relief postoperative pain and reduce the time of postoperative recovery, which has its obvious advantage comparing with traditional postoperative rehabilitation training. [ABSTRACT FROM AUTHOR]

Objective: Objective the outcome of the digital technology with decompression and bone graft for the treatment of the early and middle stage osteonecrosis of the femoral head. Methods: Retrospective analysis of 2013.01 to 2017.01 since the diagnosis and treatment of 21 patients with the early and middle stage osteonecrosis of the femoral head. The necrosis stage was divided into the Association Research Circulation Osseous (ARCO)I stage and II stage. All patients were accepting digital technology to have the preoperative preparation and guide production. Then the patients received core decompression, sequestrum erasion. impaction of artificial bone filling particles. Hip Harris Score (HHS) was used to evaluate the postoperative improvement of the hip function and the Visual Analogue Scale (VAS) to assess the intensity of pain. Results: In this study, 21 cases were followed up for at least 6 months. HHS were significantly increased when compared before surgery in the 3th month and 6th month after the treatment (P<0.05). The excellent function was ob-rained in 12 hips, good function in 7 hips, and fair function in 2 hips, the good rate was 90.5%, the survival rate of the femoral head was 100%. VAS 2 were significantly reduced when compared before surgery in the 3th month and 6th month after the treatment (P<0.05). Conclusions: Compared with the traditional treatment, the digital technology with decompression and bone graft for the treatment of the early and middle stage osteonecrosis of the femoral head has advantages in induce process of injury repair in the femoral head, promoting rhe function of hip joint, and play a very good prevention and treatment role for hip pain fracture healing time. [ABSTRACT FROM AUTHOR]

Objective： To compare the clinical, radiological outcomes and complication of unilateral pedicle screw (UPS) versus bilateral pedicle screw (BPS) in degenerative lumbar diseases after MIS-TLIF. Methods： In this study, we retrospectively analyzed 96 consecutive patients who had two-level MIS-TLIF at IA/L5 and L5/S1 from January 2012 to January 2016 and the clinical and radiological outcome and the complication were compared. Of the 96 patients for analysis, 44 patients underwent unilateral fixation, and 52 patients underwent bilateral fixation. All patients received at least 12 months fellow up. Clinical outcomes were evaluated by VAS, ODI, fusion rate, operation idex and complication. Results： There was no statistically significant difference in baseline demographic characteristics between the two groups (P>0.05). There was a significantly longer operating time, more blood loss and X-ray exposure times in BPS comparing with UPS (P<0.05). Clinical outcomes in two groups such as the pain (VAS) and Oswestry Disability Index (ODI) improved significantly after surgery (P<0.05) and no difference was found between them (P>0.05). No significant difference was found in fusion rate and total complication rate between the two groups (P>0.05). Conclusions： There was no significant difference between the two fixation methods of MIS-TLIF in clinical outcomes and complications. However, unilateral pedicle screw has some superiorities such as shorter operating time, lower cost, less blood loss and X-ray exposure. Unilateral pedicle screw might be more suitable in performing two-level pedicle screw fixation after MIS-TLIF. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the effect of using buprenorphine transdermal patch in the treatment of patients with periarthritis of shoulder. Methods: 152 cases of periarthritis of shoulder in outpatient and inpatient treatment in our hospital were collected in recent 5 years. All patients were standardized using analgesics according to the VAS score. Buprenorphine transdermal patch was added in 78 patients with moderate to severe pain. All the patients were followed up by telephone in every 3 months to quantify the changes of pain score, and the shoulder joint function was examined by using Constant-Murley shoulder function score scale at each time of outpatient follow-up. Results: 152 cases of scapulohumeral periarthritis patients were observed. Pain and shoulder joint function of the whole patients has a certain degree of mitigation and improvement. In the end of the observation, the average Constant-Murley score was up to 80 points. In the observation period, the Constant-Murley shoulder function score in the buprenorphine transdermal patch group is obviously higher than control group in the time of 6 to 18 months (P<0.05). Conclusions: Through using buprenorphine transdermal patch, it can speed up the rehabilitation and improve the quality of life of patients suffered fromperiarthritis of shoulder. [ABSTRACT FROM AUTHOR]

Objective: Use microRNA microarray chip and real time quantitative PCR method to analysis the relative expression levels of miR-15a-5p and miR-16-5p, then compare the difference between osteosarcoma paraffin block samples and peritumoral tissue. Methods: 34 cases of osteosarcoma paraffin block samples were chosen, microRNAs microarray chip and real time quantitative PCR method was used to measure the relative expression levels of miR-15a-5p and miR-16-5p. Results: microRNAs microarray chip results showed that the expression level ofmiR-15a-5p (miR-16-5p) in tumor tissue was 1.79 (1.62) times lower than the adjacent tissue. Real time quantitative PCR results showed that compare with tumor adjaccnt tissues, the expression level of miR-15a-5p and miR-16-5p was low in osteosarcoma paraffin block samples. The results were considered significant through statistical analysis (P＜0.05). Further analysis results showed that the expression level of miR-15a-5p (miR-16-5p) in tumor tissue was 3.14 (5.65) times lower than the adjacent tissue. Conclusions: miR-15a-5p and miR-16-5p was low expressed in osteosarcoma cells, which suggest that these two microRNAs could be act as oncogene. [ABSTRACT FROM AUTHOR]

Objective: To comparison the clinical efficacy and safety of transforamina 1 lumbar interbody fusion and posterior lumbar interbody fusion in treating patients with degenerative lumbar spondylolisthesis combined with lumbar spinal stenosis. Methods: A total of 90 patients with degenerative lumbar spondylolisthesis combined with lumbar spinal stenosis in our hospital from June 2013 to June 2015 were enrolled in this study. The subjects were divided into TLIF group (n=45) and PLIF group (n=45) randomly. The TLIF group were treated with TLIF. the PLIF group were treated with PLIF. The operation time, intraoperative blood loss, postoperative flow and postoperative time in bed of the two groups were compared. The VAS score and ODI score of the two groups before and after treatment were compared. The complications such as nerve root injury, infection and epidural cyst rupture of the two groups after treatment were compared. Results: The operation time, intraoperative blood loss, postoperative flow and postoperative time in bed of the TLIF group were ignificantly lower than that of the PLIF group (P<0.05). There were no significantly differences of the VAS score and ODI score of the two groups before treatment (P>0.05). After treatment, the VAS score and ODI score of the two groups were significantly lower than before treatment, and that of the TLIF group were significantly lower than the PLIF group (P<0.05). The incidence of adverse reactions after treatment of the TLIF group was significantly lower than that of the PLIF group (X2=3.873. P=0.049). Conclusion: Compared with PLIF. TLIF for patients with degenerative lumbar spondylolisthesis combined with lumbar spinal stenosis have good clinical efficacy and security, can help the recovery of the lumbar fuction. it was worthy clinical application. [ABSTRACT FROM AUTHOR]

Objective: To observe the clinical effect and safety of patients with far lateral lumbar disc herniation treated with Minimally Invasive Transforaminal Lumbar Interbody Fusion (MIS-TLIF) and tradition open-operation. Methods: The clinical and follow-up data of 61 patients from 2012.01 to 2016.01 diagnosed as far lateral lumbar disc herniation who had the operation were retrospectively analyzed. All the patients were divided into experimental group (MIS-TLIF) and control group (tradition open-operation). The clinical outcomes were evaluated by visual analogue scale (VAS) and Japanese Orthopaedic Association Scores (JOA), blood loss, operation time, times of X-ray, the time in bed, cost and complications and so on. Results: There was no difference between two groups in the VAS score and JOA score before and after the treatment while there were significant improvement (P>0.05). The blood loss, the time in bed and complications who had MIS-TLIF were significantly better compared with those in control group (P<0.05). Conclusions: MIS-TLIF as a MISS operation in the treatment of far lateral lumbar disc herniation has more particular advantages than normal treatments, which should be further application and promotion in clinic. [ABSTRACT FROM AUTHOR]

Objective: To observe the clinical effect and safety of patients with cervical spondylotic radiculopathy treated with SDS9900. Methods: The clinical and follow-up data of 165patients who were admitted in our hospital from 2013.06 to 2015.06 and diagnosed as cervical spondylotic radiculopathy treated by SDS9900 were retrospectively analyzed. All the patients were followed up at least 6 months after the treatment. The clinical outcomes were evaluated by the height of intervertebral space, visual analogue scale (VAS), NDI score and SF-36. Results: The height of intervertebral space of the patients (6.63± 0.87) broadened significantly after the treatment (P<0.05). The VAS (2.02± 0.87) and NDI (6.64± 3.17) score of SDS9900 group was significantly lower than that before the treatment (7.65± 1.01, 12.49± 7.42) (P<0.05). The quality of life were obviously improved on the day after the treatment. There was no complication in all patients. Conclusions: SDS9900 in the conservative treatment of lumbar disc herniation has more particular advantages than normal treatments, which should be further application and promotion in clinic. [ABSTRACT FROM AUTHOR]

目的探讨微小RNA-432(miR-432)对骨肉瘤细胞增殖、迁移和侵袭的影响及其作用机制。方法采用qRT-PCR技术检测人骨肉瘤细胞MG-63、人成骨细胞h FOB1.19中miR-432表达。将对数生长期MG-63细胞随机分为三组,分别转染miRNA对照质粒(miR-NC组)、miR-432抑制质粒(miR-432 inhibitor组)及miR-432过表达质粒(miR-432组),转染48 h时进行接种培养,分别检测细胞增殖(吸光度值)、迁移和侵袭情况。利用miRWalk和miRanda生物信息学软件预测miR-432靶基因可能为致癌基因CX3CL1,分别构建含有miR-432结合位点的CX3CL1基因3’-UTR区野生型(CX3CL1-wt)和突变型(CX3CL1-mut)荧光素酶报告载体。将MG-63细胞分别共转染CX3CL1-wt或CX3CL1-mut报告载体和miR-432载体(观察组),并设置共转染miR-NC载体作为对照组。转染48 h时,采用双荧光素酶试剂盒检测相对荧光素酶活性。结果 MG-63细胞中miR-432相对表达量低于h FOB1.19细胞(P<0.01)。miR-NC组、miR-432 inhibitor组、miR-432组细胞中miR-432相对表达量分别为1.00±0.01、0.47±0.03、3.62±0.03,组间比较P均<0.01。miR-432组培养24、36、48 h的细胞吸光度值均明显低于miR-NC组、miR-432 inhibitor组,miR-432 inhibitor组均明显高于miR-NC组。miR-432 inhibitor组及miR-432组细胞相对迁移率分别为(122±7)%、(65±4)%,相对侵袭率分别为(189±25)%、(49±11)%;上述指标组间比较P均<0.05。CX3CL1-wt和miR-432共转染MG-63细胞后,观察组相对荧光素酶活性低于对照组(P<0.05);CX3CL1-mut和miR-432共转染细胞后,对照组和观察组相对荧光素酶活性分别为1.00±0.11、0.93±0.13,两组比较P>0.05。结论 miR-432过表达可抑制骨肉瘤细胞增殖、迁移和侵袭,靶向调控致癌基因CX3CL1可能是其作用机制。 [ABSTRACT FROM AUTHOR]