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Background The clinical effectiveness of ultraviolet light (UV) disinfection remains unclear. This study aimed to investigate the effect of adding pulsed xenon UV (PX-UV) disinfection to the terminal cleaning protocol on the rate of methicillin-resistant Staphylococcus aureus (MRSA) acquisition at a Japanese hospital. Methods The use of a PX-UV disinfection device was added to the manual terminal cleaning protocol applied after the discharge or transfer of patients treated in the intensive and high care units. We used a Poisson regression model to examine the incidence of MRSA acquisition, based on the study period, PX-UV intervention status, unit type, and the rate of consumption of alcohol-based hand rub (ABHR). Results Approximately 86% of the rooms in the intervention units were terminally disinfected with the PX-UV device. In the intervention units, the incidence of MRSA acquisition decreased from 3.56 per 1,000 patient-days in the nonintervention period to 2.21 per 1,000 patient-days in the intervention period. Moreover, the use of PX-UV disinfection decreased the risk of MRSA acquisition (incident rate ratio: 0.556; 95% confidence interval, 0.309-0.999; P = .0497). ABHR consumption did not affect the risk of MRSA acquisition. Conclusions Adding PX-UV disinfection to terminal manual cleaning reduced the rate of MRSA acquisition.

Quantitative NMR spectroscopy (qNMR) is an essential tool in organic chemistry, with applications including reaction monitoring, mechanistic analysis, and purity determination. Establishing the correct acquisition rate for consecutive qNMR scans requires knowledge of the longitudinal relaxation time constants (T1) for all of the nuclei being monitored. We report a simple method that is about 10-fold faster than the conventional inversion recovery technique for the estimation of T1.

This paper is focused on the dynamics of the formation of conditioned responses in rats that experienced 14-day moderate magnetic shielding and were placed into a shelter setup when conducting the Barnes maze task. It was found that the acquisition rate of the conditioned active avoidance response was faster in rats under magnetic shielding conditions than that in the control animals. A direct accelerating effect of a moderate geomagnetic environment on the acquisition of the conditioned active avoidance response is thought to be extremely unlikely and the observed effect appears to be due to well-known biorhythm changes in pain sensitivity in hypomagnetic conditions associated with the development of hyperalgesia during the first week, which is an additional motivation for the formation of the conditioned active avoidance response in the Shelter setup. A two-phase change in pain sensitivity of male rats for a 14-day period was shown in the electrostimulation setup: hyperalgesia occurred during the first week and hypoalgesia was induced during the second week. The Barnes maze task did not reveal any differences between groups of control animals and experimental animals in recorded behavioral indices in hypomagnetic conditions within a 14-day period. Therefore, the accelerated formation of conditioned active avoidance response in rats under hypomagnetic conditions is most likely due to a significant increase in pain sensitivity during the first week of the experiment.

We find significant positive contemporaneous, short-run, and long-run effects of an increase in the acquisition rate on management compensation. The long-run effect of an additional deal completed each year by an average acquirer increases management’s total, equity, and cash compensation by 21 percent, 7 percent, and 22 percent, respectively. Frequent acquirers, on average, pay their management 46 percent higher in total compensation, 55 percent higher in equity-based compensation, and 6 percent higher in cash-based (short-term) compensation, relative to non-frequent acquirers. Frequent acquirers pay higher equity-based compensation than cash-based compensation compared to non-frequent acquirers. Further, the impact of the acquisition rate on management compensation is higher for value-enhancing acquirers relative to value-destroying acquirers. We find a positive bi-directional influence between acquisition frequency and management compensation. Operationally more efficient acquirers are less likely to have a higher acquisition rate. Further, frequent acquisitions do not improve an acquirer’s operational efficiency, possibly due to constant post-acquisition integration challenges. We find a positive bi-directional causality between the total q (firm value) and the acquisition frequency, which may explain a positive association between overvalued stocks and acquisition frequency. A higher market value is likely to associate with higher acquisition frequency that is further likely to be associated with higher management compensation, Further, acquisition rate has a positive impact on the market share. Acquirers with a lower market share are more likely to become frequent acquirers. A causal order appears to exist from a lower market share to a higher acquisition frequency to a higher market value of the firm to higher management compensation. The increase in shareholder value associated with a higher acquisition frequency is not likely due to a gain in the operational efficiency, but morel likely due to an increase in the growth opportunities and market share of the acquirer. We find another causal order from a lower operational efficiency to a higher acquisition rate to a higher market share to higher management compensation. The causal orders may also explain why some studies may find a negative relation between management compensation and firm performance.

The automated generation and acquisition of large-scale reaction data is crucial to recent progress in organic synthesis and pharmaceutical process development. To follow reaction progress over time, time-series of reaction data can be generated by transient flow experiments, which allow to investigate reactions in a short transient period between two steady-state operations of a continuous-flow microreactor. The acquisition of analytical data in this short transient period, however, remains challenging since separation techniques like HPLC – though able to cope with highly complex samples – are typically regarded as too slow for the required acquisition rates. We break the longstanding coupling between the timescale of the continuous-flow experiment and the acquisition rate of the analytical method with the introduction of the multiple heart-cutting interface, which allows for sample parking prior to sample analysis. Transient flow experiments are presented for the [2 + 2] cycloaddition between 1-methyl-2-quinolinone and coumarin. Competing homo- and crossdimerization reactions are quantitatively investigated in systematic reaction parameter screenings with more than 400 data points. Results provide precious insights into this complex photochemical system – not only for reaction optimization, but also for a more profound understanding of the involved chemical processes.

Background Biologging now allows detailed recording of animal movement, thus informing behavioural ecology in ways unthinkable just a few years ago. In particular, combining GPS and accelerometry allows spatially explicit tracking of various behaviours, including predation events in large terrestrial mammalian predators. Specifically, identification of location clusters resulting from prey handling allows efficient location of killing events. For small predators with short prey handling times, however, identifying predation events through technology remains unresolved. We propose that a promising avenue emerges when specific foraging behaviours generate diagnostic acceleration patterns. One such example is the caching behaviour of the arctic fox (Vulpes lagopus), an active hunting predator strongly relying on food storage when living in proximity to bird colonies. Methods We equipped 16 Arctic foxes from Bylot Island (Nunavut, Canada) with GPS and accelerometers, yielding 23 fox-summers of movement data. Accelerometers recorded tri-axial acceleration at 50 Hz while we obtained a sample of simultaneous video recordings of fox behaviour. Multiple supervised machine learning algorithms were tested to classify accelerometry data into 4 behaviours: motionless, running, walking and digging, the latter being associated with food caching. Finally, we assessed the spatio-temporal concordance of fox digging and greater snow goose (Anser caerulescens antlanticus) nesting, to test the ecological relevance of our behavioural classification in a well-known study system dominated by top-down trophic interactions. Results The random forest model yielded the best behavioural classification, with accuracies for each behaviour over 96%. Overall, arctic foxes spent 49% of the time motionless, 34% running, 9% walking, and 8% digging. The probability of digging increased with goose nest density and this result held during both goose egg incubation and brooding periods. Conclusions Accelerometry combined with GPS allowed us to track across space and time a critical foraging behaviour from a small active hunting predator, informing on spatio-temporal distribution of predation risk in an Arctic vertebrate community. Our study opens new possibilities for assessing the foraging behaviour of terrestrial predators, a key step to disentangle the subtle mechanisms structuring many predator–prey interactions and trophic networks.

Optical coherence tomography angiography (OCT-A) may help in the diagnosis of diabetic retinopathy (DR). The OCT-A field-of-view and the volume acquisition rate are limited by the number of samples. We explore the effect of reduced lateral sampling on DR classification with Deep Learning. © 2021 The Authors.

Purpose This study aims to suggest a novel strategy for assessing the activity of myopic choroidal neovascularization (mCNV) based on optical coherence tomography angiography (OCTA) and to compare it with traditional fundus fluorescein angiography as the gold standard. Methods Macular OCTA images were obtained using RTVue XR Avanti with AngioVue. Morphologic features of mCNV lesions were analyzed. Characteristics of OCTA in 41 eyes with active mCNV and 41 eyes with inactive mCNV were analyzed. Optical coherence tomography angiography parameters associated with mCNV activity and the clinical significance of their sensitivity and specificity were analyzed using fundus fluorescein angiography as the reference. Results Of the total 108 patients, 82 had OCTA images with good quality which were included in this study. Several anatomical features of the CNV lesions, including overall appearance, branching with tiny vessels, presence of anastomoses/loops, and choroidal dark halo, were considered the possible parameters associated with mCNV activity. The intra- and interobserver agreements were substantial. To evaluate the CNV activity, sensitivity of overall appearance, tiny vascular branching, and presence of anastomoses or loops were 65.9%, 82.9%, and 73.2%, respectively, whereas the specificity was 87.8%, 90.2%, and 92.7%, respectively. However, the choroidal dark halo showed low specificity (46.3%) and failed in terms of evaluating the activity of mCNV. A novel comprehensive procedure integrating branching as a major parameter and overall appearance and presence of anastomoses/loops as minor parameters was developed to evaluate mCNV activity with sensitivity of 95.1% and specificity of 85.4%. Conclusion In mCNV, the acquisition rate of clear OCTA images was 75.9%. A novel comprehensive diagnostic procedure combining mCNV appearance, vascular branching, and anastomoses/loops by OCTA may be a valuable strategy to evaluate neovascular activity in mCNV.

Advances in tandem mass spectrometry (MS/MS) acquisition rate have steadily led to increased performance in shotgun proteomics experiments. To that end, contemporary mass spectrometers are outfitted with multiple analyzers allowing for the simultaneous collection of survey (MS1) and MS/MS spectra. In the latest generation Orbitrap hybrid, MS/MS scans can be acquired at a high rate using the dual cell linear ion trap analyzer, all while the next precursor is being dissociated in a collision cell and a MS1 scan is occurring in the Orbitrap. Often overlooked in these experiments is that the ion trap scan duration is highly variable and dependent upon precursor mass. Here, we examine the use of various static mass-to-charge ratio scan ranges for ion trap MS/MS acquisition and determine performance relative to conventional dynamic mass-to-charge ratio range scanning. We demonstrate that a fixed mass-to-charge ratio scan range can generate 12% more MS/MS scans and more unique peptide identifications as compared to the standard dynamic approach, respectively.

O objetivo deste trabalho foi obter o Índice de Irregularidade Internacional (IRI) de pavimentos sobre pontes utilizando smartphones, estes equipados com acelerômetros, possibilitando a aquisição de dados de aceleração e a determinação da irregularidade através de procedimentos numéricos, consistindo na dupla integral dos dados de aceleração vertical. Foram realizadas medições em três pontes, por meio de um veículo percorrendo as mesmas, equipado com dois telefones móveis diferentes com taxa de aquisição de 100 Hz. Um dos dispositivos foi disposto no interior do veículo e outro na bandeja do sistema de suspensão. Utilizou-se outra abordagem, o Método de Nível e Mira, para a calibração da medição do tipo resposta nas pontes. As conclusões mostram uma boa convergência entre os métodos apresentados para obtenção de perfis de superfície de pontes, demostrando bom indício do uso dos smartphones como método alternativo simples e econômico, revelando fácil operação e boa produtividade.

Background:The value of decolonization as a strategy for preventing methicillin-resistantStaphylococcus aureus (MRSA) in the neonatal intensive care unit (NICU) remains to be determined.Objective:After adding decolonization to further reduce MRSA transmission in our NICU, we conducted this retrospective review to evaluate its effectiveness.Method:The review included patients who were admitted to our NICU between April 2015 and June 2018 and were eligible for decolonization including twice daily intranasal mupirocin and daily chlorhexidine gluconate bathing over 5 consecutive days. Patients were considered successfully decolonized if 3 subsequent MRSA screenings conducted at 1-week intervals were negative. The MRSA acquisition rate (AR) was calculated as hospital-acquired (HA) MRSA per 1,000 patient days (PD) and was used to measure the effectiveness of the decolonization.Results:Of the 151 MRSA patients being reviewed, 78 (51.6%) were HA-MRSA, resulting in an overall AR of 1.27 per 1,000 PD. Between April 2015 and February 2016, when only the decolonization was added, the AR was 2.38 per 1,000 PD. Between March 2016 and June 2018 after unit added a technician dedicated to the cleaning of reusable equipment, the AR decreased significantly to 0.92 per 1,000 PD (P < .05). Of the 78 patients who were started on the decolonization, 49 (62.8%) completed the protocol, 11 (14.1%) remained colonized, and 13 (16.7%) were recolonized prior to NICU discharge.Conclusion:In a NICU with comprehensive MRSA prevention measures in place, enhancing the cleaning of reusable equipment, not decolonization, led to significant reduction of MRSA transmission.

The Japan Endocrine Society (JES) has the largest ratio of female membership among societies associated with Internal Medicine in Japan; half of female members are in their 20s or 30s at present. In 2009, JES organized the "JES-We-Can" committee to promote women's career development. To evaluate the effectiveness of JES-We-Can, we investigated the gender balance of various activities at JES in fiscal 2009 and 2017. Significant gender-differences were not observed in the acquisition rate of board-certified endocrinologists (BCEs) aged

Background Health care workers (HCWs) are at high risk of contracting blood-borne infections including hepatitis B virus (HBV) infection. In Japan, all HCWs are required to receive HB vaccination before beginning work. This study aimed to investigate the dynamics of the HB surface antibody (anti-HBs) titer after a three-dose HB vaccination in HCWs and to determine effective scheduling of HB vaccination for non-responders. Methods Subjects included 832 medical and dental students who had received a three-dose HB vaccination (Bimmugen® 0.5 ml/vial). Anti-HBs was measured three times (before the third dose and 1 and 5 months after the third dose) using the CLIA method. The cut-off value of anti-HBs acquisition was 10 mIU/mL. After booster doses (three maximum) were administered to non-responders, the anti-HBs titers were measured again. Results Out of 832 students, 491 were analyzed, of which 58.9% (289) were male. Anti-HBs-positive rates before the third dose and 1 and 5 months later were 47.9%, 95.9%, and 89.0%, respectively. The relationship between the antibody titer at one month (x) and 5 months (y) was estimated by log10y = log10x − 0.134 (P Conclusion Anti-HBs titer decreases by an average of 20% within 4 months between the 1st and 5th month after the third dose. Therefore, anti-HBs titer should be measured periodically after completing the three-dose vaccination. Additionally, results suggested that booster doses are effective if administered with the same schedule as primary vaccination.

Brain-on-chip (BoC) models are tools for reproducing the native microenvironment of neurons, in order to study the (patho)physiology and drug-response of the brain. Recent developments in BoC techniques focus on steering neurons in their activity via microfabrication and via computer-steered feedback mechanisms. These cultures are often studied through calcium imaging (CI), a method for visualizing the cellular activity through infusing cells with a fluorescent dye. CAlciumImagingAnalyser 2.0 (CALIMA 2.0) is an updated version of a software tool that detects and analyzes fluorescent signals and correlates cellular activity to identify possible network formation in BoC cultures. Using three previous published data sets, it was demonstrated that CALIMA 2.0 can analyze large data sets of CI-data and interpret cell activity to help study the activity and maturity of BoC cultures. Last, an analysis of the processing speed shows that CALIMA 2.0 is sufficiently fast to process data sets with an acquisition rate up to 5 Hz in real-time on a medium-performance computer.

Summary We challenged four big brown bats to maneuver through abrupt turns in narrow corridors surrounded by dense acoustic clutter. We quantified bats' performance, sonar beam focus, and sensory acquisition rate. Performance was excellent in straight corridors, with sonar beam aim deviating less than 5° from the corridor midline. Bats anticipated an upcoming abrupt turn to the right or left by slowing flight speed and shifting beam aim to “look” proactively into one side of the corridor to identify the new flightpath. All bats mastered the right turn, but two bats consistently failed the left turn. Bats increased their sensory acquisition rate when confronting abrupt turns in both successful and failed flights. Limitations on biosonar performance reflected failures to switch beam aim and to modify a learned spatial map, rather than failures to update acquisition rate., Graphical abstract, Highlights • Echolocating bats use sonar to identify flightpaths through dense, cluttered scenes • Bats learn new flightpaths by switching beam aim before encountering obstacles • Bats see their environment in depth, Biological Sciences; Zoology; Animals; Ethology

Brillouin microscopy has been applied successfully to in-vivo corneal imaging to understand stiffness variation along different phases of keratoconus. However, the in-vivo spatial resolution is restricted due to a conflict between a slow Brillouin acquisition rate of hundreds of milliseconds and human movement during acquisition. In this research, frequency domain optical coherence tomography and image tracking are proved to be effective methods to locate points measured by Brillouin microscopy in axial and radial directions, respectively. Results show that OCT has an accuracy of better than 2 μm while image tracking has an accuracy better than 3 μm.

AIM To audit the performance of computed tomography colonography (CTC) at St Mark's Hospital against the joint British Society of Gastrointestinal and Abdominal Radiology (BSGAR) and Royal College of Radiologists (RCR) standards. MATERIALS AND METHODS A retrospective audit of all CTC studies between January 2012 to December 2017 was performed against the BSGAR/RCR standards along with additional data outwith the guidelines. Evidence was obtained from a central database, radiology information systems (RISs), picture archiving and communication systems (PACSs), and electronic patient records (EPRs). RESULTS Over the 6 years, 13,143 CTCs were performed and 12,996 (99%) were adequate or better. Of the cases 1,867 had a >6 mm polyp or cancer reported (polyp identification rate [PIR] 14%) and the positive predictive value (PPV) was 93% (1,148/1,240). Median radiation dose was 458 mGy·cm, mean additional acquisition rate was 19% (2,505/13,143), subsequent endoscopy rate was 9% (1,222/13,143) and mean interpretation time for a negative study was 34.6 minutes. Nine perforations occurred (perforation rate of 0.068%) and one was symptomatic (symptomatic perforation rate of 0.008%). For suspected cancers, the same-day endoscopy rate was 27% (96/360) and same-day staging rate was 76% (272/360). Post-imaging colorectal cancer rates (PICRC) was 3.06 per 100 cancers detected and 0.23 per 1,000 CTCs. The service was always rated “good” or higher by patients. CONCLUSION This audit shows the CTC service at St Mark's Hospital to be safe and of sufficiently high quality to meet the BSGAR/RCR standards with most outcomes equal to or above the aspirational target. Areas for service and individual reader improvement were also identified.

We present the Optimization Method for the Electron Multiplying Charge-Coupled Devices (EMCCDs) of the Acquisition System of the SPARC4 (OMASS4). The OMASS4 uses as figures of merit the signal-to-noise ratio (SNR) and the acquisition rate (AR) as a function of the operation mode of the CCDs. Three different modes of optimization are included in the OMASS4: (1) optimization of SNR only; (2) optimization of AR only; and (3) optimization of both SNR and AR simultaneously. The first two modes calculate an analytical maximization of the cost function whereas the third mode uses the bayesian optimization method to determine the optimum mode of operation. We apply the OMASS4 to find the optimum mode for observations obtained at the Pico dos Dias Observatory, Brazil, and compare the delivered modes of operation and its performance with the ones adopted by the observer. If the OMASS4 had been used as a tool to optimize the CCDs in all of these nights, it would be possible to improve their efficiency in 97.17 %, 65.08 %, and 77.66 % for the optimization modes 1, 2, and 3, respectively., Comment: 27 pages, 6 figures

Background The Scandinavian Obesity Surgery Registry (SOReg) is a national quality register that has collected data on bariatric surgery in Sweden since 2007. Objective Evaluate the acquisition rate and internal validity of entered data in SOReg as well as completeness. Settings National quality register, Sweden. Method The acquisition rate during 2012–2018 was compared with Swedish national databases, while registered data in 89 selected variables (67 mandatory) was compared with medical records of 1860 randomly selected patients from all bariatric centers (n = 39–43) reporting to SOReg. The evaluation was done by 1 independent observer. Completeness of data in the entire registry for the same time period was studied. Results The acquisition rate was 97.4%, because 33,716 of 34,628 patients recorded in the National Inpatient Registry were registered in SOReg. Exact agreement of reabstracted data was seen in 99.0% of 100,200 unique entries. All studied variables had an almost perfect agreement with Cohen's kappa ranging from .87–1, that is, >.81 according to Landis and Koch criteria. In addition, .3% (n = 301) missing data entries were discovered, mostly in administrative variables. In the mandatory variables, overall completeness was high; however, declining with time in parallel with reduced follow-up rate, 50% at 5 years. Conclusion The high acquisition rate and internal validity implies that SOReg reflects Swedish bariatric surgery on a nationwide basis. Hence, SOReg data can be used to monitor quality of care and in research. The overall completeness could be improved by a higher registration rate during long-term follow-up.

Carriage dynamics of drug-resistant bacteria, especially within households, are poorly understood. This limits the ability to develop effective interventions for controlling the spread of antimicrobial resistance in the community. Two groups consisting of: (i) patients with urinary tract infection requiring antimicrobial treatment; and (ii) patients who were not prescribed antimicrobial treatment were prospectively recruited at three European sites: Antwerp (Belgium), Geneva (Switzerland) and Lodz (Poland). Each index patient and up to three additional household members provided faecal samples at baseline, completion of antimicrobial therapy (or 7-10 days after the first sample for the non-exposed) and 28 days after the second sample. We analysed household-level and individual-level fluoroquinolone resistant Enterobacteriaceae (FQR-E) acquisition and carriage data using Bayesian multi-state Markov models. At the individual level, we estimated a median baseline FQR-E acquisition rate of 0.006 (95%CrI = [0.004, 0.01]) per day, and a median duration of carriage of 24.4 days (95% CrI=[15.23,41.38]). Nitrofurantoin exposure was associated with a reduced rate of FQR-E acquisition (HR=0.28, 95%CrI=[0.14,0.56]), while fluoroquinolone exposure had no clear association with rates of FQR-E acquisition (HR=1.43, 95% CrI=[0.81,2.53]) at individual level. There was evidence that rates of FQR-E acquisition varied by site, and coming from Lodz was associated with a higher acquisition rate (HR=3.56, 95% CrI=[1.92, 6.34]). Prolonged duration of carriage was associated with exposure to fluoroquinolone or nitrofurantoin during the study, use of any antimicrobial agent in the prior 12 months and travel to endemic regions. At household level, we found strong evidence of positive association between FQR-E acquisition and fluoroquinolone exposure (HR=3.43, 95% CrI=[1.51,7.74]). There was weak evidence of negative association between FQR-E acquisition and nitrofurantoin exposure (HR=0.42, 95%CrI=[0.12, 1.24]. Similar to the individual level, carriage duration was also associated with antimicrobial exposure at the household level. Our study has identified within household contacts as an important route for FQR-E transmission and highlights the need for prioritising household focused interventions to control FQR-E spread.

Optical frequency comb with evenly spaced lines over a broad bandwidth has revolutionized the fields of optical metrology and spectroscopy. Despite dual-comb spectroscopy (DCS) offers a superb overall performance on spectral resolution, measurement bandwidth and refresh rate, these parameters are still constrained by the Nyquist-limited trade-off. Here, we propose a novel DCS system to overcome this limitation with tens of spectral acquisition rate improvement, which is built with two electro-optic frequency combs seeded by an ultralinearly swept light source. The proposed scheme records a spectrum spanning 304 GHz with a spectral resolution of 10 kHz in 1.6 ms, which achieves a spectral sampling-rate of 1.9×1010 points/s. We also demonstrate the 100-averaged results with 28.9 dB signal-to-noise ratio for high sensitivity measurement. The demonstration shows great prospect for precise measurement with significant performance breakthrough.

Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection, allowing shorter dwell times and thus increasing imaging speed. Negative bias potential has previously been used to tune penetration depth in block-face imaging. However, optimization of negative bias potential for application in thin section imaging will be needed prior to routine use and application in large-scale EM. Here, we present negative bias potential optimized through a combination of simulations and empirical measurements. We find that the use of a negative bias potential generally results in improvement of image quality and signal-to-noise ratio (SNR). The extent of these improvements depends on the presence and strength of a magnetic immersion field. Maintaining other imaging conditions and aiming for the same image quality and SNR, the use of a negative stage bias can allow for a 20-fold decrease in dwell time, thus reducing the time for a week long acquisition to less than 8 hours. We further show that negative bias potential can be applied in an integrated correlative light electron microscopy (CLEM) application, allowing fast acquisition of a high precision overlaid LM-EM dataset. Application of negative stage bias potential will thus help to solve the current bottleneck of image acquisition of large fields of view at high resolution in large-scale microscopy.