76 results on '"Xi-Feng Zhang"'
Search Results
2. Identification of unique transcriptomic signatures through integrated multispecies comparative analysis and WGCNA in bovine oocyte development
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Fa-Li Zhang, Wei-Dong Li, Geng Zhang, Min Zhang, Zhao-Jun Liu, Ke-Xin Zhu, Qing-Chun Liu, Shu-Er Zhang, Wei Shen, and Xi-Feng Zhang
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Genetics ,Biotechnology - Abstract
Background Cattle (Bos taurus) are a major large livestock, however, compared with other species, the transcriptional specificity of bovine oocyte development has not been emphasised. Results To reveal the unique transcriptional signatures of bovine oocyte development, we used integrated multispecies comparative analysis and weighted gene co-expression network analysis (WGCNA) to perform bioinformatic analysis of the germinal follicle (GV) and second meiosis (MII) gene expression profile from cattle, sheep, pigs and mice. We found that the expression levels of most genes were down-regulated from GV to MII in all species. Next, the multispecies comparative analysis showed more genes involved in the regulation of cAMP signalling during bovine oocyte development. Moreover, the green module identified by WGCNA was closely related to bovine oocyte development. Finally, integrated multispecies comparative analysis and WGCNA picked up 61 bovine-specific signature genes that participate in metabolic regulation and steroid hormone biosynthesis. Conclusion In a short, this study provides new insights into the regulation of cattle oocyte development from a cross-species comparison.
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- 2023
3. Construction of a Silver Nanoparticle Complex and its Application in Cancer Treatment
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Wan Fen Li, Hao Hai Ma, Pei Yu Dong, Chen Lin Liang, Long Li, and Xi Feng Zhang
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Nanomedicine has been used in tumor treatment and research due to its advantages of targeting, controlled release and high absorption rate. Silver nanoparticle (AgNPs), with the advantages of small particle size, and large specific surface area, are of great potential value in suppressing and killing cancer cells. Methods: AgNPs–polyethyleneimine (PEI) –folate (FA) (AgNPs–PF) were synthesised and characterised by several analytical techniques. The ovarian cancer cell line Skov3 was used as the cell model to detect the tumor treatment activity of AgNPs, AgNPs–PF and AgNPs+ AgNPs–PF. Results: Results shown that AgNPs–PF were successfully constructed with uniform particle size of 50–70 nm. AgNPs, AgNPs–PF, AgNPs–PF+ AgNPs all showed a certain ability to inhibit cancer cell proliferation, increase reactive oxygen species and decrease the mitochondrial membrane potential. All AgNPs, AgNPs–PF, AgNPs+ AgNPs–PF promoted DNA damage in Skov3 cells, accompanied by the generation of histone RAD51 and γ-H2AX site, and eventually leading to the apoptosis of Skov3 cells. The combination of AgNPs–PF and AgNPs had a more pronounced effect than either material alone. Conclusion: This study is to report that the combination of AgNPs+ AgNPs–PF can cause stronger cytotoxicity and induce significantly greater cell death compared to AgNPs or AgNPs–PF alone in Skov3 cells. Therefore, the combined application of drugs could be the best way to cancer treatment.
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- 2022
4. Case Report: Adult degenerative scoliosis in two patients treated with percutaneous spinal endoscopic-assisted lumbar interbody fusion and percutaneous pedicle screw fixation
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Jian-wei Du, Lei-ming Zhang, Yu-qiu Yan, Ya-ning Zhang, Xue-qin Rong, Song-hua Xiao, and Xi-feng Zhang
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Surgery - Abstract
Adult degenerative scoliosis (ADS) is a serious disease that often affects middle-aged and elderly people. ADS does not only cause sagittal and coronal deformity of the lumbar spine but also causes severe back and leg pain secondary to the compression of the neural structures. Open surgery remains the main method for correcting the occurring deformity and decompression of the neural structures; however, its benefit is limited in cases of large trauma. Minimally invasive spinal (MIS) surgery is an alternative method that has recently witnessed rapid development. It has the advantage of providing rapid recovery with less trauma as compared to conventional open surgery. We report two cases of ADS treated with percutaneous spinal endoscopic-assisted lumbar interbody fusion (EALIF) and percutaneous pedicle screw fixation. Both cases had moderate deformities of the lumbar spine (load-sharing classification 4–7 points) with severe back and leg pain, and they underwent successful MIS surgery. At 6 months of follow-up, the visual analog scale and Oswestry disability index scores of both patients improved and the deformity was corrected. For moderate ADS, percutaneous spinal EALIF and percutaneous pedicle screw fixation may achieve an effective correction of the deformity with direct decompression of neural structures.
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- 2023
5. Identification of apoptotic pathways in zearalenone-treated mouse sertoli cells
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Hu-He, Chao, Lei, Wang, Hao-Hai, Ma, Ai-Hong, Zhao, Hong-Wei, Xiao, and Xi-Feng, Zhang
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Male ,Mammals ,Mice ,Sertoli Cells ,Animals ,Zearalenone ,Apoptosis ,Estrogens, Non-Steroidal ,Mycotoxins ,Toxicology - Abstract
Zearalenone (ZEN), one of the most prevalent non-steroidal oestrogenic mycotoxins, is primarily produced by Fusarium fungi. Due to its toxicity as an oestrogenic compound and wide distribution in feed and foods, the reproductive toxicology of ZEN exposure is of public concern. The aim of the present study was to investigate the effect of ZEN on Sertoli cells to identify apoptotic pathways induced by this compound. We found that ZEN reduced the viability and caused apoptosis in Sertoli cells in vitro. Notably, we observed that such effects were associated with a significant increase in reactive oxygen species (ROS) and the number of cells that showed positive staining for γH2AX and RAD51, enzymes essential for repairing DNA damage. There was a parallel decrease in the expression of occludin and connexin 43, proteins that are present in the testis-blood barrier and gap junctions of Sertoli cells, respectively. Overall, the present study confirms that ZEN exposure can have serious deleterious effects on mammalian Sertoli cells and offers novel insight about its molecular targets in these cells.
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- 2022
6. Spatiotemporal Pattern of Ecosystem Respiration in China Estimated by Integration of Machine Learning With Ecological Understanding
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Lang Han, Gui‐Rui Yu, Zhi Chen, Xian‐Jin Zhu, Wei‐Kang Zhang, Tie‐Jun Wang, Li Xu, Shi‐Ping Chen, Shao‐Min Liu, Hui‐Min Wang, Jun‐Hua Yan, Jun‐Lei Tan, Fa‐Wei Zhang, Feng‐Hua Zhao, Ying‐Nian Li, Yi‐Ping Zhang, Li‐Qing Sha, Qing‐Hai Song, Pei‐Li Shi, Jiao‐Jun Zhu, Jia‐Bing Wu, Zhong‐Hui Zhao, Yan‐Bin Hao, Xi‐Bin Ji, Liang Zhao, Yu‐Cui Zhang, Shi‐Cheng Jiang, Feng‐Xue Gu, Zhi‐Xiang Wu, Yang‐Jian Zhang, Li Zhou, Ya‐Kun Tang, Bing‐Rui Jia, Gang Dong, Yan‐Hong Gao, Zheng‐De Jiang, Dan Sun, Jian‐Lin Wang, Qi‐Hua He, Xin‐Hu Li, Fei Wang, Wen‐Xue Wei, Zheng‐Miao Deng, Xiang‐Xiang Hao, Xiao‐Li Liu, Xi‐Feng Zhang, Xing‐Guo Mo, Yong‐Tao He, Xin‐Wei Liu, Hu Du, and Zhi‐Lin Zhu
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Atmospheric Science ,Global and Planetary Change ,Environmental Chemistry ,General Environmental Science - Published
- 2022
7. Antioxidant and Biological Activities of the Lotus Root Polysaccharide-Iron (III) Complex
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Shuai Yuan, Pei-Yu Dong, Hao-Hai Ma, Sheng-Lin Liang, Long Li, and Xi-Feng Zhang
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Anemia, Iron-Deficiency ,Iron ,Organic Chemistry ,Pharmaceutical Science ,Antioxidants ,Hormones ,Analytical Chemistry ,Mice ,Chemistry (miscellaneous) ,Polysaccharides ,lotus root ,polysaccharide ,polysaccharide iron ,antioxidant ,metabolome ,microbiota ,Drug Discovery ,Spectroscopy, Fourier Transform Infrared ,Molecular Medicine ,Humans ,Animals ,Steroids ,Physical and Theoretical Chemistry - Abstract
In this study, the synthesis parameters of the lotus root polysaccharide iron complex (LRPF) were determined and optimized by response surface methodology. Under the optimum preparation conditions, the pH of the solution was 9, the ratio of M (trisodium citrate): m (lotus root polysaccharide) was 0.45, the reaction time was 3 h. UV spectroscopy, thermogravimetry, FT-IR spectroscopy, X-ray diffraction, CD, and NMR were used for the characterization of the LRPF. LRPF has good stability and easily releases iron ions under artificial gastrointestinal conditions. LRPF exhibited antioxidant activity in vitro and can significantly improve the antioxidant activity in vivo. In addition, LRPF has a good effect in the treatment of iron deficiency anemia in model mice, impacts the gut microbiome, and reduces the iron deficiency-induced perniciousness by regulating steroid hormone biosynthesis. Therefore, LRPF can be used as a nutritional supplement to treat and prevent iron-deficiency anemia and improve human immunity.
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- 2022
8. Structural modification and biological activities of carboxymethyl Pachymaran
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Hao-Hai Ma, You-Yu Yan, Shuai Yuan, and Xi-Feng Zhang
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chemistry.chemical_classification ,Circular dichroism ,ABTS ,polysaccharide selenium ,Poria cocos ,Nutrition. Foods and food supply ,Chemistry ,polysaccharide zinc ,Metal ions in aqueous solution ,polysaccharides ,Substrate (chemistry) ,Nuclear magnetic resonance spectroscopy ,Polysaccharide ,carbohydrates (lipids) ,chemistry.chemical_compound ,TX341-641 ,Chelation ,polysaccharide iron ,Fourier transform infrared spectroscopy ,antitumor ,Food Science ,Nuclear chemistry - Abstract
Polysaccharides are good chelating agents for metal ions, which are often used to synthesize polysaccharide metal ion complexes. With carboxymethyl pachymaran (CMP) as the substrate, carboxymethyl pachymaran iron (CMPF), carboxymethyl pachymaran selenium (CMPS), and carboxymethyl pachymaran zinc (CMPZ) were synthesized by response surface methodology, and their biological characteristics were studied. The results showed that the CMP was a β‐polysaccharide, and the degree of carboxymethylation was 0.6352. The polysaccharide metal ion complexes were characterized by physicochemical methods, scanning electron microscopy, Fourier transform infrared spectroscopy, circular dichroism spectroscopy, and nuclear magnetic resonance spectroscopy. All the polysaccharides and complexes possessed antioxidant activity in vitro with scavenging activities to ABTS, superoxide anions, and ferrous ions. CMPF, CMPS, and CMPZ caused significant inhibition of A2780 cell proliferation, promoted the production of reactive oxygen species, and induced apoptosis in a human ovarian cancer cell line (A2780 cells). These results suggest that the CMP complex may be an effective candidate drug for cancer treatment in the field of functional food and pharmacology.
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- 2021
9. Mapping Chinese annual gross primary productivity with eddy covariance measurements and machine learning
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Xian-Jin Zhu, Gui-Rui Yu, Zhi Chen, Wei-Kang Zhang, Lang Han, Qiu-Feng Wang, Shi-Ping Chen, Shao-Min Liu, Hui-Min Wang, Jun-Hua Yan, Jun-Lei Tan, Fa-Wei Zhang, Feng-Hua Zhao, Ying-Nian Li, Yi-Ping Zhang, Pei-Li Shi, Jiao-Jun Zhu, Jia-Bing Wu, Zhong-Hui Zhao, Yan-Bin Hao, Li-Qing Sha, Yu-Cui Zhang, Shi-Cheng Jiang, Feng-Xue Gu, Zhi-Xiang Wu, Yang-Jian Zhang, Li Zhou, Ya-Kun Tang, Bing-Rui Jia, Yu-Qiang Li, Qing-Hai Song, Gang Dong, Yan-Hong Gao, Zheng-De Jiang, Dan Sun, Jian-Lin Wang, Qi-Hua He, Xin-Hu Li, Fei Wang, Wen-Xue Wei, Zheng-Miao Deng, Xiang-Xiang Hao, Yan Li, Xiao-Li Liu, Xi-Feng Zhang, and Zhi-Lin Zhu
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Machine Learning ,Carbon Sequestration ,Soil ,Environmental Engineering ,Climate Change ,Environmental Chemistry ,Carbon Dioxide ,Pollution ,Waste Management and Disposal ,Ecosystem ,Carbon - Abstract
Annual gross primary productivity (AGPP) is the basis for grain production and terrestrial carbon sequestration. Mapping regional AGPP from site measurements provides methodological support for analysing AGPP spatiotemporal variations thereby ensures regional food security and mitigates climate change. Based on 641 site-year eddy covariance measuring AGPP from China, we built an AGPP mapping scheme based on its formation and selected the optimal mapping way, which was conducted through analysing the predicting performances of divergent mapping tools, variable combinations, and mapping approaches in predicting observed AGPP variations. The reasonability of the selected optimal scheme was confirmed by assessing the consistency between its generating AGPP and previous products in spatiotemporal variations and total amount. Random forest regression tree explained 85 % of observed AGPP variations, outperforming other machine learning algorithms and classical statistical methods. Variable combinations containing climate, soil, and biological factors showed superior performance to other variable combinations. Mapping AGPP through predicting AGPP per leaf area (PAGPP) explained 86 % of AGPP variations, which was superior to other approaches. The optimal scheme was thus using a random forest regression tree, combining climate, soil, and biological variables, and predicting PAGPP. The optimal scheme generating AGPP of Chinese terrestrial ecosystems decreased from southeast to northwest, which was highly consistent with previous products. The interannual trend and interannual variation of our generating AGPP showed a decreasing trend from east to west and from southeast to northwest, respectively, which was consistent with data-oriented products. The mean total amount of generated AGPP was 7.03 ± 0.45 PgC yr
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- 2022
10. A green and highly efficient method of extracting polyphenols from Lilium davidii var. unicolor Salisb using deep eutectic solvents
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Xiaoyun Wang, Qingting Zhang, Xi feng Zhang, Hui Xue, and Ji Zhang
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biology ,Chemistry ,General Chemical Engineering ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,biology.organism_classification ,020401 chemical engineering ,Polyphenol ,Lilium davidii ,Organic chemistry ,0204 chemical engineering ,0210 nano-technology ,Eutectic system - Abstract
It is important to find pollution-free, eco-friendly solvents to replace traditional organic solvents. As a new class of medium, deep eutectic solvents (DESs) provide a source of breaking through t...
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- 2021
11. Lotus root polysaccharide inhibits the growth of ovarian cancer cells by blocking the cell cycle
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Pei-Yu Dong, Sheng-Lin Liang, Long Li, Jing Liu, and Xi-Feng Zhang
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- 2023
12. Multi-omics analysis reveals that iron deficiency impairs spermatogenesis by gut-hormone synthesis axis
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Fa-Li, Zhang, Shuai, Yuan, Pei-Yu, Dong, Hao-Hai, Ma, Massimo, De Felici, Wei, Shen, and Xi-Feng, Zhang
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Male ,Gut microbiome ,Multi-omics ,Settore BIO/17 ,Iron ,Iron deficiency ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,Iron Deficiencies ,General Medicine ,Pollution ,Hormones ,Mice ,CeRNA ,Animals ,Metabolomics ,Spermatogenesis - Abstract
Considering that research has mainly focussed on how excessive iron supplementation leads to reproductive cytotoxicity, there is a lack of in-depth research on reproductive system disorders caused by iron deficiency. To gain a better understanding of the effects of iron deficiency on the reproductive system, especially spermatogenesis, we first constructed a mouse model of iron deficiency. We employed multi-omic analysis, including transcriptomics, metabolomics, and microbiomics, to comprehensively dissect the impact of iron deficiency on spermatogenesis. Moreover, we verified our findings in detail using western blot, immunofluorescence, immunohistochemistry, qRT-PCR and other techniques. Microbiomic analysis revealed altered gut microbiota in iron-deficient mice, and functional predictive analysis showed that gut microbiota can regulate spermatogenesis. The transcriptomic data indicated that iron deficiency directly alters expression of meiosis-related genes. Transcriptome data also revealed that iron deficiency indirectly regulates spermatogenesis by affecting hormone synthesis, findings confirmed by metabolomic data, western blot and immunofluorescence. Interestingly, competing endogenous RNA networks also play a vital role in regulating spermatogenesis after iron deficiency. Taken together, the data elucidate that iron deficiency impairs spermatogenesis and increases the risk of male infertility by affecting hormone synthesis and promoting gut microbiota imbalance.
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- 2022
13. Polysaccharide extracted from Huperzia serrata using response surface methodology and its biological activity
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Xi-Feng Zhang and Yan-Ni Feng
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Antioxidant ,Surface Properties ,medicine.medical_treatment ,Antineoplastic Agents ,Fraction (chemistry) ,02 engineering and technology ,Chemical Fractionation ,Raw material ,Polysaccharide ,Biochemistry ,Antioxidants ,03 medical and health sciences ,Polysaccharides ,Structural Biology ,medicine ,Response surface methodology ,Molecular Biology ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Chromatography ,biology ,Chemistry ,Monosaccharides ,Extraction (chemistry) ,Temperature ,Water ,Huperzia ,Biological activity ,General Medicine ,Huperzia serrata ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Microscopy, Electron, Scanning ,0210 nano-technology - Abstract
In this study, Huperzia serrata polysaccharide (HSP) fraction was isolated using response surface methodology (RSM) and Box-Behnken design (BBD). The extraction time, temperature and ratio of water to raw material were employed effects. And properties of four polysaccharide (60%-HSP, 70%-HSP, 80%-HSP and 90%-HSP) were evaluated. The results indicated that the optimal extraction conditions were the following: 3.07 h, 49.46 °C and a liquid material ratio of 20.73:1. The four HSP presented irregular aggregation of shape. And all HSP exhibited antioxidant and anticancer activities.
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- 2020
14. lncRNA‑MM2P downregulates the production of pro‑inflammatory cytokines in acute gouty arthritis
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Ju Liu, Xueyong Li, Ying Zou, Xi-feng Zhang, Mingliang Qiu, Feng Ye, Sen-guo Ji, Xiuzhen Wen, Jiangxia Zheng, and Jin Lei
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Lipopolysaccharides ,0301 basic medicine ,Cancer Research ,Small interfering RNA ,monosodium urate-induced inflammation ,Lipopolysaccharide ,THP-1 Cells ,Cell ,Regulator ,Down-Regulation ,Inflammation ,Models, Biological ,Biochemistry ,Proinflammatory cytokine ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Downregulation and upregulation ,acute gouty arthritis ,Genetics ,medicine ,Animals ,Humans ,Molecular Biology ,long non-coding RNA-MM2P ,Gene knockdown ,Arthritis, Gouty ,business.industry ,Articles ,Uric Acid ,RAW 264.7 Cells ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,chemistry ,regulator ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Immunology ,Cytokines ,Molecular Medicine ,RNA, Long Noncoding ,medicine.symptom ,business - Abstract
Acute gouty arthritis (AGA) is characterized by the accumulation of pro‑inflammatory cytokines, which are immunological responses to monosodium urate (MSU) crystals. It has been demonstrated that long non‑coding RNA (lncRNA)‑MM2P is a novel regulator of M2 polarization of macrophages. The aim of the present study was to investigate whether lncRNA‑MM2P regulates the MSU‑induced inflammatory process. In cell models of RAW 264.7 and THP‑1‑derived macrophages, decreased expression of lncRNA‑MM2P was observed in lipopolysaccharide‑ and MSU‑treated macrophages, which was accompanied with obvious inflammatory responses. Using small interfering RNA to knockdown lncRNA‑MM2P led to the upregulation of MSU‑mediated inflammatory responses, both in RAW 264.7 and THP‑1‑derived macrophages. In conclusion, lncRNA‑MM2P could be an important regulator of MSU‑induced inflammation, and therefore could be involved in the development of AGA.
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- 2020
15. Polysaccharides from Pyracantha fortuneana and its biological activity
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You-Yu Yan, Shu Chang, Xi-Feng Zhang, Wang Qing, Yi-Lan Yao, Hongxun Wang, Yang Yi, Ge Feng, and Wang Limei
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Antioxidant ,DNA damage ,medicine.medical_treatment ,Apoptosis ,Pyracantha fortuneana ,02 engineering and technology ,Polysaccharide ,Biochemistry ,Antioxidants ,03 medical and health sciences ,Polysaccharides ,Structural Biology ,Cell Line, Tumor ,medicine ,Humans ,Cytotoxicity ,Molecular Biology ,030304 developmental biology ,Ovarian Neoplasms ,chemistry.chemical_classification ,0303 health sciences ,Reactive oxygen species ,biology ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Antineoplastic Agents, Phytogenic ,Neoplasm Proteins ,chemistry ,Cancer cell ,Female ,Pyracantha ,Reactive Oxygen Species ,0210 nano-technology ,DNA Damage - Abstract
This study used response surface methodology to determine the optimal conditions for extraction of polysaccharides from Pyracantha. fortuneana (PSPF), and studied the mechanism of PSPF-inducing apoptosis in human ovarian carcinoma Skov3 cells. Response surface methodology (RSM) were adopted to extract PSPF. The maximum value of polysaccharide yield was obtained under these optimal conditions. PSPF had good potential as an antioxidant. Exposure of cells to PSPF resulted in cytotoxicity through the induction of apoptosis, and the reactive oxygen species were increased, mitochondrial membrane potential decreased, DNA damage (detected as γ- H2AX and RAD51 foci) was observed in Skov3 cells. In addition, PSPF could induce apoptosis of cancer cells. Therefore, PSPF should be explored as novel potential antioxidants and an anti-tumor drug in a clinical setting.
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- 2020
16. Single-cell transcriptomic profiling to evaluate the effects of Di(2-ethylhexyl)phthalate exposure on early meiosis of female mouse germ cells
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Yu, Tian, Ye, Zhang, Pei-Yu, Dong, Yong-Hong, Sun, Ai-Hong, Zhao, Wei, Shen, and Xi-Feng, Zhang
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Mammals ,Environmental Engineering ,Health, Toxicology and Mutagenesis ,Public Health, Environmental and Occupational Health ,General Medicine ,General Chemistry ,Pollution ,Meiosis ,Mice ,Germ Cells ,Diethylhexyl Phthalate ,Oocytes ,Animals ,Humans ,Environmental Chemistry ,Female ,Transcriptome - Abstract
Di(2-ethylhexyl)phthalate (DEHP) has proven characteristics of an endocrine-disrupting compound (EDC), which can threaten the reproductive health of humans and other animals. In mammals, a series of chromosomal events occur during the meiotic stage of oocytes. External toxins may enter the body and cause infertility and other related diseases. Therefore, it is crucial to explore the influence of DEHP exposure on the molecular mechanism of germ cell meiosis. We used single-cell RNA sequencing (scRNA-seq) to analyse the ovaries of foetal mice at embryonic day 12.5 (E12.5) and E14.5 after maternal DEHP exposure. DEHP exposure further activated the pathways related to DNA repair in germ cells, increased the expression of genes related to DNA damage and changed the developmental trajectory of germ cells. DEHP exposure may affect the proliferation of pregranulosa (PG) cells. Moreover, DEHP exposure altered the signal transduction between PG cells and germ cells. We showed that DEHP affects meiosis by causing DNA damage in oocytes and disrupting the signal transduction between PG cells and germ cells. These results provide a strong theoretical basis for the prevention and treatment of DEHP-mediated female reproductive health problems.
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- 2022
17. Structural modification and biological activities of carboxymethyl
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You-Yu, Yan, Shuai, Yuan, Hao-Hai, Ma, and Xi-Feng, Zhang
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carbohydrates (lipids) ,polysaccharide selenium ,Poria cocos ,polysaccharide zinc ,polysaccharides ,polysaccharide iron ,Original Research ,antitumor - Abstract
Polysaccharides are good chelating agents for metal ions, which are often used to synthesize polysaccharide metal ion complexes. With carboxymethyl pachymaran (CMP) as the substrate, carboxymethyl pachymaran iron (CMPF), carboxymethyl pachymaran selenium (CMPS), and carboxymethyl pachymaran zinc (CMPZ) were synthesized by response surface methodology, and their biological characteristics were studied. The results showed that the CMP was a β‐polysaccharide, and the degree of carboxymethylation was 0.6352. The polysaccharide metal ion complexes were characterized by physicochemical methods, scanning electron microscopy, Fourier transform infrared spectroscopy, circular dichroism spectroscopy, and nuclear magnetic resonance spectroscopy. All the polysaccharides and complexes possessed antioxidant activity in vitro with scavenging activities to ABTS, superoxide anions, and ferrous ions. CMPF, CMPS, and CMPZ caused significant inhibition of A2780 cell proliferation, promoted the production of reactive oxygen species, and induced apoptosis in a human ovarian cancer cell line (A2780 cells). These results suggest that the CMP complex may be an effective candidate drug for cancer treatment in the field of functional food and pharmacology., Carboxymethyl pachymaran as the substrate, the carboxymethyl pachymaran iron, carboxymethyl pachymaran selenium, and carboxymethyl pachymaran zinc were synthesized by response surface methodology, and their biological characteristics were studied.
- Published
- 2021
18. SNP discovery and functional annotation in the Panax japonicus var. major transcriptome
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Ding-Ping Bai, Jian Li, and Xi-Feng Zhang
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chemistry.chemical_classification ,Genetics ,biology ,General Chemical Engineering ,Cytochrome P450 ,Single-nucleotide polymorphism ,02 engineering and technology ,General Chemistry ,Panax japonicus ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Transcriptome ,chemistry ,Glycosyltransferase ,biology.protein ,SNP ,0210 nano-technology ,Triterpenoid saponin ,Reference genome - Abstract
Due to the lack of a Panax japonicus var. major reference genome, we assembled a reference transcriptome from P. japonicus C. A. Mey transcriptome sequencing data, and 203 283 unigenes were obtained. In this study, with the assistance from the Trinity, Bowtie2 and SAMtools softwares, 218 465 single nucleotide polymorphisms (SNPs) were identified by mapping the Illumina sequences to the reference transcriptome. The SNP forms included 126 262 transformations and 92 203 transversions. A large number of SNP loci were associated with triterpenoid saponin synthesis: 54 SNPs were associated with cytochrome P450, one with glycosyl transferase and 94 with the biosynthesis of the triterpenoid saponin backbone.
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- 2019
19. [Spatial-temporal Variations and the Regulators of Nitrate Status in Shallow Groundwater of the Typical Mountainous Agricultural Watershed in the Upper Reaches of the Yangtze River]
- Author
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Nan, Jiang, Ming-Hua, Zhou, Hong, Li, Zi-Yang, Li, Xi-Feng, Zhang, and Bo, Zhu
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Nitrates ,Rivers ,Groundwater ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Shallow groundwater is the main drinking water supply for the mountainous area in the upper reaches of the Yangtze River, while its quality is often degraded by nitrate (NO
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- 2020
20. Extraction of polysaccharides from
- Author
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Shuai, Yuan, Chang-Yuan, Xu, Jie, Xia, Yan-Ni, Feng, Xi-Feng, Zhang, and You-Yu, Yan
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codonopsis ,antioxidation ,polysaccharide ,response surface method ,Original Research - Abstract
Codonopsis pilosula is a kind of traditional Chinese medicine used to treat weak spleens, stomach problems, anemia, and fatigue. Polysaccharide is one of main components of Codonopsis pilosula. In this study, response surface methodology (RSM) was used to optimize the extraction parameters of Codonopsis pilosula polysaccharides (CPP) by fermentation. The exaction temperature (°C), yeast liquid volume (2 mg/ml, ml), and time (h) were employed effects. Results indicated that the best extraction conditions were the following: extraction temperature 24.75°C, yeast liquid volume 2.96 ml (5.92 mg), and a fermentation time of 21.03 hr. After purification with DE52 and Sephadex G‐100, the molecular structure was determined by ultraviolet–visible (UV) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) (1H and 13C). The monosaccharide composition of CPP1 was determined to be mannose (1.76%), glucose (97.38%), and arabinose (0.76%). CPP1 exhibited high antioxidant activities in scavenging ABTS radicals, ferreous ions, and superoxide ion radicals. Thus, CPP1 could be used as an antioxidant or functional food., Response surface methodology (RSM) was used to optimize the extraction parameters of Codonopsis pilosula polysaccharides (CPPs) by fermentation.
- Published
- 2020
21. Production of a codonopsis polysaccharide iron complex and evaluation of its properties
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Ge Feng and Xi-Feng Zhang
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Antioxidant ,medicine.medical_treatment ,Iron ,02 engineering and technology ,Polysaccharide ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Structural Biology ,Cell Line, Tumor ,medicine ,Humans ,Response surface methodology ,Molecular Biology ,Codonopsis ,030304 developmental biology ,chemistry.chemical_classification ,Membrane Potential, Mitochondrial ,0303 health sciences ,Chromatography ,biology ,Codonopsis pilosula ,Chemistry ,Extraction (chemistry) ,Water extraction ,General Medicine ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Antineoplastic Agents, Phytogenic ,Proteoglycans ,0210 nano-technology ,Citric acid ,Reactive Oxygen Species - Abstract
A water extraction and alcohol precipitation method was applied to extract polysaccharides from Codonopsis pilosula (CPP), response surface methodology was used to optimize the extraction conditions and synthesis of C. pilosula polysaccharide iron (CPPI), and the properties of CPPI were evaluated. The optimum extraction conditions for CPP were as follows: liquid-solid ratio of 29.39 mL/g, time of 1.25 h and temperature of 62.84 °C. The optimum synthesis conditions for CPPI were pH 8.9, temperature 70.30 °C and the ratio of citric acid to CPP1 of 2.95. An HPSEC-MALLS-RID system, UV spectroscopy, FT-IR spectroscopy and NMR were used for characterization of the polysaccharide. CPPI exhibited antioxidant activity in vitro and a relatively strong inhibitory effect on A2780 cells growth. After CPPI treatment, the reactive oxygen species increased, the mitochondrial membrane potential decreased, and DNA damage was observed in A2780 cells. Therefore, CPPI should be explored as a potential antioxidant and an antitumor drug in a clinical setting.
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- 2020
22. Low-dose diethylhexyl phthalate exposure does not impair the expressive patterns of epigenetics-related genes and DNA methylation of breast cancer-related genes in mouse mammary glands
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Fang-Nong Lai, Yong Zhao, Bo Li, Shun-Feng Cheng, Xi-Feng Zhang, Jing-Cai Liu, Wei Shen, Lan Li, and Ling Li
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0301 basic medicine ,endocrine system ,Health, Toxicology and Mutagenesis ,Mammary gland ,Public Health, Environmental and Occupational Health ,Phthalate ,Disease ,Biology ,Toxicology ,medicine.disease ,Pathology and Forensic Medicine ,Andrology ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,medicine.anatomical_structure ,Breast cancer ,chemistry ,DNA methylation ,medicine ,Epigenetics ,General Pharmacology, Toxicology and Pharmaceutics ,Estrogen receptor alpha ,Gene - Abstract
Di-(2-ethylhexyl) phthalate (DEHP), as an endocrine-disrupting chemical (EDC), is widely used in plasticizer and other productions. Ubiquitous human exposure to DEHP has been proposed to be a potential risk to public health. Developmental exposure to DEHP could alter epigenetic programming and result in adult-onset disease. In this study, we investigated whether DEHP exposure to pregnant mice affected epigenetic changes as a result of increase in breast cancer incidence. Our results showed that the expression of total 143 epigenetics-related genes in mammary gland cells, have no significantly altered after short time and low-dose treated with DEHP from 0.5 days post-coitum (dpc) to 3.5 dpc of pregnant mice. DNA methylation status of some neoplastic development genes, such as EGFr, Esr1, Pgr, Fos and Rassf5 also had no obvious change. These finding showed no impact of DEHP on the expressive patterns of epigenetics-related genes and DNA methylation of breast cancer-related genes in pregnant mouse mammary gland cells.
- Published
- 2018
23. Production of Pyracantha Polysaccharide-Iron(III) Complex and Its Biologic Activity
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Li Wanfen, Hao-Hai Ma, Xi-Feng Zhang, and Shuai Yuan
- Subjects
antioxidant ,Antioxidant ,Radical ,medicine.medical_treatment ,polysaccharides ,Pharmaceutical Science ,Antineoplastic Agents ,02 engineering and technology ,Polysaccharide ,Ferric Compounds ,Article ,response surface optimisation ,Analytical Chemistry ,lcsh:QD241-441 ,03 medical and health sciences ,chemistry.chemical_compound ,Pyracantha ,lcsh:Organic chemistry ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,Physical and Theoretical Chemistry ,Cytotoxicity ,030304 developmental biology ,Trisodium citrate ,chemistry.chemical_classification ,Biological Products ,0303 health sciences ,ABTS ,biology ,Superoxide ,Organic Chemistry ,Free Radical Scavengers ,021001 nanoscience & nanotechnology ,biology.organism_classification ,chemistry ,Chemistry (miscellaneous) ,Molecular Medicine ,polysaccharide iron ,0210 nano-technology ,Nuclear chemistry - Abstract
In this study, the optimum synthetic process of the Pyracantha polysaccharide-iron (PPI) complex was studied via response surface methodology (RSM). Its antioxidant and anti-cancer activities were also investigated. It was demonstrated that the optimal conditions for the synthetic process of the complex were as follows: a pH of 8.9, a reaction temperature of 70 °C and a trisodium citrate:polysaccharide ratio of 1:2. PPI were analysis by UV, FTIR, SEM, CD, XRD, TGA and NMR. PPI was able to scavenge the metal ion, ABTS and free radicals of the superoxide anion, demonstrating its potential antioxidant activity. PPI was found to display cytotoxicity to Skov3 cells, as shown by its ability to induce apoptosis and alter gene expression in Skov3 cells. These findings show than PPI may represent a novel antioxidant and chemotherapeutic drug.
- Published
- 2021
24. Biofabrication of a novel biomolecule-assisted reduced graphene oxide: an excellent biocompatible nanomaterial
- Author
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Xi-Feng Zhang and Sangiliyandi Gurunathan
- Subjects
nicotinamide ,Pharmaceutical Science ,Apoptosis ,Biocompatible Materials ,02 engineering and technology ,01 natural sciences ,law.invention ,Nanomaterials ,Mice ,Tissue engineering ,International Journal of Nanomedicine ,law ,Drug Discovery ,Cells, Cultured ,Original Research ,Membrane Potential, Mitochondrial ,chemistry.chemical_classification ,Reverse Transcriptase Polymerase Chain Reaction ,Oxides ,General Medicine ,021001 nanoscience & nanotechnology ,tight junction proteins ,graphene oxide ,Graphite ,0210 nano-technology ,alkaline phosphatase ,Biofabrication ,Materials science ,Biocompatibility ,Blotting, Western ,Biophysics ,Bioengineering ,Nanotechnology ,Real-Time Polymerase Chain Reaction ,010402 general chemistry ,reduced graphene oxide ,Biomaterials ,biocompatibility ,Animals ,RNA, Messenger ,Cell Proliferation ,Solanum tuberosum ,Plant Extracts ,Graphene ,Biomolecule ,Organic Chemistry ,Fibroblasts ,Embryo, Mammalian ,Nanostructures ,0104 chemical sciences ,chemistry ,Cell culture ,Nanocarriers - Abstract
Xi-Feng Zhang,1 Sangiliyandi Gurunathan2 1College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, People’s Republic of China; 2Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul, Republic of Korea Abstract: Graphene has been shown much interest, both in academics and industry due to its extraordinary physical, chemical, and biological proprieties. It shows great promises in biotechnological and biomedical applications as an antibacterial and anticancer agent, nanocarrier, sensor, etc. However, many studies demonstrated the toxicity of graphene in several cell lines, which is an obstacle to its use in biomedical applications. In this study, to improve the biocompatibility of graphene, we used nicotinamide (NAM) as a reducing and stabilizing agent to catalyze the reduction of graphene oxide (GO) to reduced graphene oxide (rGO). The resulted smaller-sized GO (NAM-rGO) showed excellent biocompatibility with mouse embryonic fibroblast cells, evidenced by various cellular assays. Furthermore, NAM-rGO had no effect on mitochondrial membrane permeability and caspase-3 activity compared to GO. Reverse transcription polymerase chain reaction analysis allowed us to identify the molecular mechanisms responsible for NAM-rGO-induced biocompatibility. NAM-rGO significantly induced the expression of genes encoding tight junction proteins (TJPs) such as zona occludens-1 (Tjp1) and claudins (Cldn3) without any effect on the expression of cytoskeleton proteins. Furthermore, NAM-rGO enhances the expression of alkaline phosphatase (ALP) gene, and it does this in a time-dependent manner. Overall, our study depicted the molecular mechanisms underlying NAM-rGO biocompatibility depending on upregulation of TJPs and ALP. This potential quality of graphene could be used in diverse applications including tissue regeneration and tissue engineering. Keywords: biocompatibility, graphene oxide, nicotinamide, reduced graphene oxide, tight junction proteins, alkaline phosphatase
- Published
- 2016
25. Cytotoxic effects of ZnO nanoparticles on mouse testicular cells
- Author
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Zhiguo Liu, Wei Ge, Xi-Feng Zhang, Sangiliyandi Gurunathan, Zhe Han, Wei Shen, Qi Yan, and Massiomo De Felici
- Subjects
Male ,0301 basic medicine ,Medicine (General) ,ZnO nanoparticle ,Metal Nanoparticles ,Pharmaceutical Science ,Apoptosis ,02 engineering and technology ,Microscopy, Atomic Force ,X-Ray Diffraction ,International Journal of Nanomedicine ,Spectroscopy, Fourier Transform Infrared ,Drug Discovery ,Nanotechnology ,Cytotoxicity ,Spectroscopy ,Cells, Cultured ,Original Research ,chemistry.chemical_classification ,Microscopy ,Cultured ,Settore BIO/17 ,Leydig cell ,Atomic Force ,Leydig Cells ,General Medicine ,021001 nanoscience & nanotechnology ,Sertoli cell ,medicine.anatomical_structure ,Zinc Oxide ,0210 nano-technology ,mice ,Materials science ,DNA damage ,Cells ,Biophysics ,Bioengineering ,Electron ,Biomaterials ,03 medical and health sciences ,R5-920 ,Microscopy, Electron, Transmission ,In vivo ,medicine ,Transmission ,Animals ,Humans ,Reactive oxygen species ,Sertoli Cells ,Leydig cells ,Sertoli cells ,DNA Damage ,Mice ,Reactive Oxygen Species ,Organic Chemistry ,technology, industry, and agriculture ,In vitro ,030104 developmental biology ,chemistry ,Fourier Transform Infrared - Abstract
Zhe Han,1,* Qi Yan,1,* Wei Ge,2 Zhi-Guo Liu,1 Sangiliyandi Gurunathan,3 Massimo De Felici,4 Wei Shen,2 Xi-Feng Zhang1 1College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, People’s Republic of China; 2Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, People’s Republic of China; 3Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, Republic of Korea; 4Department of Biomedicine and Prevention, University of Rome “Tor Vergata”, Rome, Italy *These authors contributed equally tothis work Background: Nanoscience and nanotechnology are developing rapidly, and the applications of nanoparticles (NPs) have been found in several fields. At present, NPs are widely used in traditional consumer and industrial products, however, the properties and safety of NPs are still unclear and there are concerns about their potential environmental and health effects. The aim of the present study was to investigate the potential toxicity of ZnO NPs on testicular cells using both in vitro and in vivo systems in a mouse experimental model. Methods: ZnO NPs with a crystalline size of 70 nm were characterized with various analytical techniques, including ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, and atomic force microscopy. The cytotoxicity of the ZnO NPs was examined in vitro on Leydig cell and Sertoli cell lines, and in vivo on the testes of CD1 mice injected with single doses of ZnO NPs.Results: ZnO NPs were internalized by Leydig cells and Sertoli cells, and this resulted in cytotoxicity in a time- and dose-dependent manner through the induction of apoptosis. Apoptosis likely occurred as a consequence of DNA damage (detected as γ-H2AX and RAD51 foci) caused by increase in reactive oxygen species associated with loss of mitochondrial membrane potential. In addition, injection of ZnO NPs in male mice caused structural alterations in the seminiferous epithelium and sperm abnormalities.Conclusion: These results demonstrate that ZnO NPs have the potential to induce apoptosis in testicular cells likely through DNA damage caused by reactive oxygen species, with possible adverse consequences for spermatogenesis and therefore, male fertility. This suggests that evaluating the potential impacts of engineered NPs is essential prior to their mass production, to address both the environmental and human health concerns and also to develop sustainable and safer nanomaterials. Keywords: ZnO nanoparticle, Sertoli cells, Leydig cells, mice
- Published
- 2016
26. Percutaneous endoscopic decompression for calcified thoracic disc herniation using a novel T rigid bendable burr
- Author
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Wenying Lv, Xi-Feng Zhang, Gang Cheng, Leiming Zhang, Jianning Zhang, and Deng-Yuan Wang
- Subjects
medicine.medical_specialty ,Percutaneous ,business.industry ,food and beverages ,Mean age ,General Medicine ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,medicine ,Endoscopic decompression ,In patient ,Neurology (clinical) ,business ,Thoracic disc ,030217 neurology & neurosurgery - Abstract
Background The basic endoscopic instruments are not suitable for removing calcified or hard discs in patients with thoracic disc herniations (TDH). We describe a percutaneous endoscopic technique for the treatment of calcified TDH using an endoscopic drill system with a T rigid bendable burr. Methods Eleven patients (8 males, mean age 42.1 years) with single-segmental calcified TDH were treated with percutaneous endoscopic surgeries. Results Our technique using this endoscopic drill system with a T rigid bendable burr is safe and effective for the treatment of calcified TDH. Conclusions Percutaneous endoscopic decompression using the T rigid bendable burr is a safe and reproducible surgical procedure for the treatment of calcified TDH.
- Published
- 2019
27. SNP discovery and functional annotation in the
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Jian, Li, Ding-Ping, Bai, and Xi-Feng, Zhang
- Abstract
Due to the lack of a
- Published
- 2018
28. Di(2-ethylhexyl)phthalate: Adverse effects on folliculogenesis that cannot be neglected
- Author
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Teng Zhang, Wei Shen, Xi-Feng Zhang, and Massimo De Felici
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Epidemiology ,Health, Toxicology and Mutagenesis ,Population ,010501 environmental sciences ,Biology ,01 natural sciences ,Oogenesis ,03 medical and health sciences ,Follicle ,chemistry.chemical_compound ,Internal medicine ,medicine ,Epigenetics ,Ovarian follicle ,education ,Genetics (clinical) ,0105 earth and related environmental sciences ,education.field_of_study ,Phthalate ,Oocyte ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Folliculogenesis - Abstract
Primordial follicle formation and the subsequent transition of follicles through primary and secondary stages constitute crucial events of oogenesis. In particular, in mammals, defects in the processes that precede and accompany the formation of the primordial follicle pool can affect the size of this population significantly, while alterations in follicle activation, growth and maturation can result in premature depletion of the follicle reserve or cause follicle arrest at immature stages. Over the last decade, in vitro and in vivo approaches have been used to provide evidence that exposure to di(2-ethylhexyl)phthalate(DEHP), the most widely used plasticizer, has a deleterious effect on various stages of folliculogenesis in rodents. There is growing concern, supported by epidemiological and experimental data, that DEHP may have similar effects in women. This article reviews the evidence, with particular reference to our own findings, that DEHP may actually exert a variety of adverse effects on mammalian folliculogenesis from early to final stages of oogenesis, including altered development of the primordial germ cells, impaired fetal oocyte survival and meiotic progression, reduced oocyte nest breakdown, acceleration of primordial follicle activation, altered follicle steroidogenesis and increased follicle atresia. These effects can cause serious complications for reproductive and nonreproductive women's health. In addition, emerging data indicate that phthalates, including DEHP, may cause subtle epigenetic changes in germ cells that can be transmitted to subsequent generations, with potential negative effects on human health. Environ. Mol. Mutagen. 57:589-604, 2016. © 2016 Wiley Periodicals, Inc.
- Published
- 2016
29. Di (2-ethylhexyl) phthalate impairs steroidogenesis in ovarian follicular cells of prepuberal mice
- Author
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Wei Shen, Lan Li, Massimo De Felici, Yu-Ping Liu, Fang-Nong Lai, Hong Chen, Paul W. Dyce, Jing-Cai Liu, Xue-Lian Liu, Jun-Yu Ma, and Xi-Feng Zhang
- Subjects
0301 basic medicine ,endocrine system ,medicine.medical_specialty ,Health, Toxicology and Mutagenesis ,Inbred Strains ,Mice, Inbred Strains ,Ovary ,010501 environmental sciences ,Biology ,Toxicology ,01 natural sciences ,Di (2-ethylhexyl) phthalate ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Ovarian Follicle ,Diethylhexyl Phthalate ,Internal medicine ,Follicular phase ,medicine ,Folliculogenesis ,Pre-puberty mice ,Steroidogenesis ,Animals ,Female ,Gene Expression Regulation ,Granulosa Cells ,Oligonucleotide Array Sequence Analysis ,Puberty ,Steroids ,Androstenedione ,Ovarian follicle ,0105 earth and related environmental sciences ,Settore BIO/17 ,Phthalate ,General Medicine ,Antral follicle ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Theca - Abstract
Di (2-ethylhexyl) phthalate (DEHP) is a plasticizer which is widely used in the manufacture of plastics. As a common environmental contaminant and recognized endocrine disrupting chemical, DEHP is able to deregulate the functions of a variety of tissues, including the reproductive system both in males and females. In order to investigate the possible effects of DEHP on the first wave of folliculogenesis, occurring in the mouse ovary postnatally, mice were administered 20 or 40 μg/kg DEHP through intraperitoneal injection at days 5, 10 and 15 post partum (dpp). Following DEHP treatment the gene expression profile of control and exposed ovaries was compared by microarray analyses at 20 dpp. We found that in the exposed ovaries DEHP significantly altered the transcript levels of several immune response and steroidogenesis associated genes. In particular, DEHP significantly decreased the expression of genes essential for androgen synthesis by theca cells including Lhcgr, Cyp17a1, Star and Ldlr. Immunohistochemistry and immune flow cytometry confirmed reduced expression of LHCGR and CYP17A1 proteins in the exposed theca cells. These effects were associated to a significant reduction in ovarian concentrations of progesterone, 17β-estradiol and androstenedione along with a reduction of LH in the serum. Although we did not find a significant reduction of the number of primary, secondary or antral follicles in the DEHP exposed ovaries when compared to controls, we did observe that theca cells showed an altered structure of the nuclear envelope, fewer mitochondria, and mitochondria with a reduced number of cristae. Collectively, these results demonstrate a deleterious effect of DEHP exposure on ovarian steroidogenesis during the first wave of folliculogenesis that could potentially affect the correct establishment of the hypothalamic-pituitary-ovarian axis and the onset of puberty.
- Published
- 2016
30. Mycotoxin zearalenone exposure impairs genomic stability of swine follicular granulosa cells
- Author
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Shun-Feng Cheng, Rui-Qian Zhang, Xiao-Feng Sun, Xi-Feng Zhang, Yong Zhao, Xue-Lian Liu, Tian-Yu Zhang, Rui-Ying Wu, Lan Li, and Wei Shen
- Subjects
0301 basic medicine ,Genomic stability ,Granulosa cells ,DNA Repair ,Somatic cell ,DNA damage ,DNA repair ,Swine ,Blotting, Western ,Estrogen receptor ,Apoptosis ,Biology ,Applied Microbiology and Biotechnology ,Genomic Instability ,Flow cytometry ,03 medical and health sciences ,Western blot ,medicine ,Animals ,Estrogens, Non-Steroidal ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Cells, Cultured ,Cell Proliferation ,medicine.diagnostic_test ,Dose-Response Relationship, Drug ,Reverse Transcriptase Polymerase Chain Reaction ,Sequence Analysis, RNA ,fungi ,food and beverages ,Cell Biology ,Molecular biology ,In vitro ,Culture Media ,030104 developmental biology ,Zearalenone ,Female ,Developmental Biology ,Research Paper - Abstract
Zearalenone (ZEA), a metabolite of Fusarium fungi, is commonly found on moldy grains. Because it can competitively combine to estrogen receptor to disrupt estrogenic signaling, it has been reported to have serious adverse effects on animal reproduction systems. In order to explore the genotoxic effects of ZEA exposure on ovarian somatic cells, porcine granulosa cells were exposed to 10 μM and 30 μM ZEA for 24 or 72 h in vitro. The results showed that ZEA exposure for 24 h remarkably reduced the proliferation of porcine granulosa cells in a dose-dependent manner as determined by MTT analysis and flow cytometry. Furthermore, exposure to ZEA for 72 h induced apoptosis, and RNA sequence analysis also revealed that the expression of apoptosis related genes were altered. RT-qPCR, immunofluorescence and western blot analysis further confirmed the expression of DNA damage and repair related genes (γ-H2AX, BRCA1, RAD51 and PRKDC) were increased in ZEA exposed granulosa cells. When the estrogen antagonist, tamoxifen, was added with ZEA in the culture medium, the DNA damage and repairment by ZEA returned to normal level. Collectively, these results illustrate that ZEA disrupts genome stability and inhibits growth of porcine granulosa cells via the estrogen receptors which may promote granulosa cell apoptosis when the DNA repair system is not enough to rescue this serious damage.
- Published
- 2017
31. Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells
- Author
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Ding-Ping Bai, Xi-Feng Zhang, Guo-Liang Zhang, Sangiliyandi Gurunathan, and Yifan Huang
- Subjects
0301 basic medicine ,autophagy ,Cell Survival ,Biophysics ,Pharmaceutical Science ,Nanoparticle ,zinc oxide nanoparticles ,Bioengineering ,Antineoplastic Agents ,DNA fragmentation ,human ovarian cancer cells SKOV3 ,medicine.disease_cause ,Biomaterials ,03 medical and health sciences ,Drug Delivery Systems ,mitochondrial membrane potential ,Downregulation and upregulation ,Microscopy, Electron, Transmission ,X-Ray Diffraction ,International Journal of Nanomedicine ,Cell Line, Tumor ,Drug Discovery ,Spectroscopy, Fourier Transform Infrared ,medicine ,Humans ,Viability assay ,Cytotoxicity ,Original Research ,Membrane Potential, Mitochondrial ,Ovarian Neoplasms ,Chemistry ,Organic Chemistry ,Autophagy ,apoptosis ,General Medicine ,Caspase 9 ,Surface coating ,Oxidative Stress ,030104 developmental biology ,Apoptosis ,Nanoparticles ,Female ,Zinc Oxide ,Reactive Oxygen Species ,Oxidative stress - Abstract
Ding-Ping Bai,1,* Xi-Feng Zhang,2,* Guo-Liang Zhang,3,4 Yi-Fan Huang,1 Sangiliyandi Gurunathan5 1Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou, China; 2College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China; 3Dong-E-E-Jiao Co., Ltd., Shandong, China; 4National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Shandong, China; 5Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul, Republic of Korea *These authors contributed equally to this work Background: Zinc oxide nanoparticles (ZnO NPs) are frequently used in industrial products such as paint, surface coating, and cosmetics, and recently, they have been explored in biologic and biomedical applications. Therefore, this study was undertaken to investigate the effect of ZnO NPs on cytotoxicity, apoptosis, and autophagy in human ovarian cancer cells (SKOV3). Methods: ZnO NPs with a crystalline size of 20 nm were characterized with various analytical techniques, including ultraviolet-visible spectroscopy, X-ray diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, and atomic force microscopy. The cytotoxicity, apoptosis, and autophagy were examined using a series of cellular assays. Results: Exposure of cells to ZnO NPs resulted in a dose-dependent loss of cell viability, and the characteristic apoptotic features such as rounding and loss of adherence, enhanced reactive oxygen species generation, and loss of mitochondrial membrane potential were observed in the ZnO NP-treated cells. Furthermore, the cells treated with ZnO NPs showed significant double-strand DNA breaks, which are gained evidences from significant number of γ-H2AX and Rad51 expressed cells. ZnO NP-treated cells showed upregulation of p53 and LC3, indicating that ZnO NPs are able to upregulate apoptosis and autophagy. Finally, the Western blot analysis revealed upregulation of Bax, caspase-9, Rad51, γ-H2AX, p53, and LC3 and downregulation of Bcl-2. Conclusion: The study findings demonstrated that the ZnO NPs are able to induce significant cytotoxicity, apoptosis, and autophagy in human ovarian cells through reactive oxygen species generation and oxidative stress. Therefore, this study suggests that ZnO NPs are suitable and inherent anticancer agents due to their several favorable characteristic features including favorable band gap, electrostatic charge, surface chemistry, and potentiation of redox cycling cascades. Keywords: zinc oxide nanoparticles, human ovarian cancer cells SKOV3, mitochondrial membrane potential, apoptosis, DNA fragmentation, autophagy
- Published
- 2017
32. Effect of Preparation Conditions on Evaluation of Porous Silicon’s Morphology and Coalition with its Optical Property
- Author
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Hong Xia Dong, Ke Wei Li, Yu Wang, and Xi Feng Zhang
- Subjects
Aqueous solution ,Photoluminescence ,Materials science ,Nanotechnology ,General Medicine ,Electrolyte ,Porous silicon ,chemistry.chemical_compound ,Hydrofluoric acid ,chemistry ,Chemical engineering ,Etching (microfabrication) ,Wafer ,Layer (electronics) - Abstract
This article presents the dependence of porous silicon (PSi) morphology on preparation conditions and the coalition between morphology and optical properties. p-type (100) silicon wafers were etched in an electrolyte incorporating 1:1 (by volume) ethanol and aqueous solution of hydrofluoric acid (40 wt. %) at various etching current densities and times. Detailed information about evolution of PSi morphology with variation of preparation conditions was investigated by field emission scanning electron microscopy (FESEM) and atomic force microscope (AFM). The results have shown that the pore sizes of PSi are gradually increasing, and the thicknesses of PSi layer are increased with the speed of about 11-12 nm/s as the etching duration increased. Addition, the optimal photoluminescence of PSi is achieved in room temperature with the strongest photoluminescence spectra when the corrosion current density was 30 mA / cm2 with 30 min etching time.
- Published
- 2015
33. Porous Silicon as Functionalized Material for Biomedical Application
- Author
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Yuan Yuan Chou, Xi Feng Zhang, and Hong Xia Dong
- Subjects
Materials science ,Biocompatibility ,Silicon ,business.industry ,technology, industry, and agriculture ,chemistry.chemical_element ,Nanotechnology ,General Medicine ,equipment and supplies ,Porous silicon ,Nanomaterials ,chemistry ,Specific surface area ,Microelectronics ,Nanometre ,Electronics ,business - Abstract
As a novel functional nanomaterial, porous silicon has many unique properties, such as its unique optical characteristics, biocompatibility, abundance, mechanical, electronic properties, silicon microelectronic compatibility, filtration, nanometer micropore controllable growth and large specific surface area, which enhance its prospect in the biological analysis, immune virus detection, environmental, food industry and so on, and has attracted world interests in the fields of materials science, biology, medicine, and electronics. In this work the application of porous silicon in the fields of biological and biomedical has been introduced with depth and width of researching on its.
- Published
- 2014
34. Di-(2-ethylhexyl) phthalate and bisphenol A exposure impairs mouse primordial follicle assembly in vitro
- Author
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Lin-Qing Wang, Lan Li, Yang Zhou, Xi-Feng Zhang, Teng Zhang, Massimo De Felici, Hong Chen, Wei Shen, Guo-Qing Qin, and Xun-Si Qin
- Subjects
endocrine system ,medicine.medical_specialty ,urogenital system ,Epidemiology ,Health, Toxicology and Mutagenesis ,Phthalate ,Biology ,Oocyte ,Oogenesis ,Transplantation ,chemistry.chemical_compound ,Endocrinology ,medicine.anatomical_structure ,FIGLA ,chemistry ,Internal medicine ,medicine ,Folliculogenesis ,Spermatogenesis ,hormones, hormone substitutes, and hormone antagonists ,Genetics (clinical) ,Germ cell - Abstract
Bisphenol-A (BPA) and diethylhexyl phthalate (DEHP) are estrogenic compounds widely used in commercial plastic products. Previous studies have shown that exposure to such compounds have adverse effects on various aspects of mammalian reproduction including folliculogenesis. The objective of this study was to examine the effects of BPA and DEHP exposure on primordial follicle formation. We found that germ cell nest breakdown and primordial follicle assembly were significantly reduced when newborn mouse ovaries were exposed to 10 or 100 μM BPA and DEHP in vitro. Moreover, BPA and DEHP exposure increased the number of TUNEL positive oocytes and the mRNA level of the pro-apoptotic gene Bax in oocytes. These effects were associated with decreased expression of oocyte specific genes such as LIM homeobox 8 (Lhx8), factor in the germline alpha (Figla), spermatogenesis and oogenesis helix-loop-helix (Sohlh2), and newborn ovary homeobox (Nobox). Interestingly, BPA and DEHP exposure also prevented DNA demethylation of CpG sites of the Lhx8 gene in oocytes, a process normally associated with folliculogenesis. Finally, folliculogenesis was severely impaired in BPA and DEHP exposed ovaries after transplantation into the kidney capsules of immunodeficient mice. In conclusion, BPA and DEHP exposures impair mouse primordial follicle assembly in vitro.
- Published
- 2014
35. Notch pathway regulates female germ cell meiosis progression and early oogenesis events in fetal mouse
- Author
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Xun-Si Qin, Massimo De Felici, Bo Pan, Xi-Feng Zhang, Yan-Min Feng, Shun-Feng Cheng, Lan Li, Chun-Lei Chen, Wei Shen, and Gui-Jin Liang
- Subjects
Notch signaling pathway ,Apoptosis ,Tretinoin ,Biology ,oogenesis ,meiosis ,mouse ,notch ,Stra8 ,Methylation ,Oogenesis ,Mice ,DAZL ,Fetus ,Meiosis ,Report ,medicine ,Animals ,Receptor, Notch1 ,Promoter Regions, Genetic ,Molecular Biology ,Adaptor Proteins, Signal Transducing ,Genetics ,Settore BIO/17 ,Days post coitum ,Dipeptides ,Cell Biology ,Oocyte ,Cell biology ,medicine.anatomical_structure ,Oocytes ,Female ,Carbamates ,Folliculogenesis ,Germ cell ,Signal Transduction ,Developmental Biology - Abstract
A critical process of early oogenesis is the entry of mitotic oogonia into meiosis, a cell cycle switch regulated by a complex gene regulatory network. Although Notch pathway is involved in numerous important aspects of oogenesis in invertebrate species, whether it plays roles in early oogenesis events in mammals is unknown. Therefore, the rationale of the present study was to investigate the roles of Notch signaling in crucial processes of early oogenesis, such as meiosis entry and early oocyte growth. Notch receptors and ligands were localized in mouse embryonic female gonads and 2 Notch inhibitors, namely DAPT and L-685,458, were used to attenuate its signaling in an in vitro culture system of ovarian tissues from 12.5 days post coitum (dpc) fetus. The results demonstrated that the expression of Stra8, a master gene for germ cell meiosis, and its stimulation by retinoic acid (RA) were reduced after suppression of Notch signaling, and the other meiotic genes, Dazl, Dmc1, and Rec8, were abolished or markedly decreased. Furthermore, RNAi of Notch1 also markedly inhibited the expression of Stra8 and SCP3 in cultured female germ cells. The increased methylation status of CpG islands within the Stra8 promoter of the oocytes was observed in the presence of DAPT, indicating that Notch signaling is probably necessary for maintaining the epigenetic state of this gene in a way suitable for RA stimulation. Furthermore, in the presence of Notch inhibitors, progression of oocytes through meiosis I was markedly delayed. At later culture periods, the rate of oocyte growth was decreased, which impaired subsequent primordial follicle assembly in cultured ovarian tissues. Taken together, these results suggested new roles of the Notch signaling pathway in female germ cell meiosis progression and early oogenesis events in mammals.
- Published
- 2014
36. Grb7 is over-expressed in cervical cancer and facilitate invasion and inhibit apoptosis in cervical cancer cells
- Author
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Hao-Bin Wang, Hong-Bing Zhao, Xue-Lin Jia, and Xi-Feng Zhang
- Subjects
0301 basic medicine ,Adult ,Cell signaling ,Uterine Cervical Neoplasms ,Apoptosis ,Kaplan-Meier Estimate ,Pathology and Forensic Medicine ,HeLa ,03 medical and health sciences ,0302 clinical medicine ,Growth factor receptor ,medicine ,Biomarkers, Tumor ,Humans ,Neoplasm Invasiveness ,Aged ,Cervical cancer ,Gene knockdown ,biology ,GRB7 ,Cell Biology ,Middle Aged ,medicine.disease ,biology.organism_classification ,Prognosis ,Survival Analysis ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Tumor progression ,030220 oncology & carcinogenesis ,GRB7 Adaptor Protein ,biology.protein ,Cancer research ,Disease Progression ,Female ,HeLa Cells - Abstract
Growth factor receptor bound protein-7 (Grb7) is a multi-domain adaptor protein that is co-opted by numerous tyrosine kinases involved in various cellular signaling. The objective of this study was to investigate the expression of Grb7 and its clinicopathological significance in cervical cancer. Utilising immunohistochemical staining, we examined the expression of Grb7 in 120 cases of human cervical cancer tissue and 10 cases of adjacent non-cancerous cervical tissue. The positive rate of Grb7 protein expression was 34.2%, which was significantly higher than that in adjacent non-cancerous cervical tissue (0%, p
- Published
- 2016
37. Synthesis, Physicochemical, and Tribological Characterization of Nano Lubricant Contained Cu Nanocolloid as Additive
- Author
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Hong Xia Dong, Min Qin, Ke Wei Li, Zhen Ye Xu, and Xi Feng Zhang
- Subjects
Materials science ,Liquid paraffin ,General Engineering ,chemistry.chemical_element ,Polyethylene ,Ascorbic acid ,Copper ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,Agglomerate ,Particle size ,Lubricant ,Composite material ,Tribometer - Abstract
Monocrystalline Cu nanocolloid with 4.3 nm average particle size and 2.3~9.5 nm particle size distribution was successfully synthesized in situ by liquor-phase reduction synthesis, using copper acetate tetrahydrate as precursor, ascorbic acid as reductant, polyethylene sorbitan monooleate (Tween-80) as modifier, and liquid paraffin as solvent. The as-prepared copper nanocolloid was characterized by XRD, SAED, and TEM. Nano-lubricating oil with excellent dispersibility, chemical stability, and compatibility with other additives verified by TG-DSC, was achieved via adding as-prepared nano-copper into Mobil 1 5w-30 lubricating oil. The nano-lube’s physiochemical properties were characterized according to Chinese national standard. The tribological behavior of nano-lubricant was investigated on ball-on-disk UMT-Ⅱ tribometer and four-ball tribometer. The worn surface morphology was analyzed by means of SEM and EDS. Experimental results show as follows. The Mobil 1 5w-30 lube’s tribological property is markedly enhanced with the as-prepared copper nanocolloid as its additive. The as-prepared nano-Cu agglomerate discontinuous deposited, extrude, melt, spread, and form a film in micro-defects on the worn surfaces, then improve the friction-reducing, anti-wear ability, extreme pressure value, and extreme working ability of Mobil 1 5w-30 lubricant.
- Published
- 2013
38. Effects of diethylhexyl phthalate (DEHP) given neonatally on spermatogenesis of mice
- Author
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Hong-Ying Zhang, Yanni Feng, Liu Wang, Yan-Min Feng, Wei Shen, Xun-Si Qin, Xi-Feng Zhang, Yong-De Chen, Lan Li, and Teng Zhang
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Time Factors ,Male mice ,Biology ,Semen analysis ,Mice ,chemistry.chemical_compound ,Meiosis ,Diethylhexyl Phthalate ,Internal medicine ,Testis ,Genetics ,medicine ,Animals ,Spermatogenesis ,Molecular Biology ,Chromosome Aberrations ,medicine.diagnostic_test ,Phthalate ,General Medicine ,Sperm ,Semen Analysis ,Endocrinology ,medicine.anatomical_structure ,Animals, Newborn ,Gene Expression Regulation ,chemistry ,CYP17A1 ,Germ cell - Abstract
We previously demonstrated that the effects of diethylhexyl phthalate (DEHP) alter reproduction function on male mice. Immature male mice were treated daily with DEHP from postnatal day 7-21, 7-35, 7-49, in a dose-dependent manner. As results, both the quality and quantity of spermatozoa were decreased in 60-day-old mice. The results by RT-PCR analysis indicated that DDx3Y, Usp9Y, RBM, E1F1AY, EGF, FSHR and EGFR genes were down-regulated, and LHR, Cyp17a1 and Cyp19a1 were down-regulated in response to DEHP. These genes were selected based on their markedly increased or decreased expression levels. However, DEHP had no effect on the meiotic process and recombination levels in male mouse germ cells. Treatment with DEHP induced histopathological changes in the testes. Taken together, these results provide a new insight into the molecular mechanisms underlying the detrimental impacts of DEHP in humans and wildlife.
- Published
- 2013
39. Theranostics Aspects of Various Nanoparticles in Veterinary Medicine
- Author
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Yifan Huang, Xin-Yu Lin, Xi-Feng Zhang, and Ding-Ping Bai
- Subjects
Veterinary Medicine ,0301 basic medicine ,Engineering ,Veterinary medicine ,diagnostic ,Growth promotion ,Nanoparticle ,Review ,02 engineering and technology ,Theranostic Nanomedicine ,Catalysis ,lcsh:Chemistry ,Inorganic Chemistry ,03 medical and health sciences ,Health care ,Physical and Theoretical Chemistry ,lcsh:QH301-705.5 ,Molecular Biology ,Spectroscopy ,Micellar nanoparticles ,Animal health ,business.industry ,animal production ,Organic Chemistry ,Animal production ,General Medicine ,021001 nanoscience & nanotechnology ,Polymeric nanoparticles ,Nanostructures ,Computer Science Applications ,livestock ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,drug delivery ,antimicrobial ,Nanomedicine ,nanoparticles ,0210 nano-technology ,business - Abstract
Nanoscience and nanotechnology shows immense interest in various areas of research and applications, including biotechnology, biomedical sciences, nanomedicine, and veterinary medicine. Studies and application of nanotechnology was explored very extensively in the human medical field and also studies undertaken in rodents extensively, still either studies or applications in veterinary medicine is not up to the level when compared to applications to human beings. The application in veterinary medicine and animal production is still relatively innovative. Recently, in the era of health care technologies, Veterinary Medicine also entered into a new phase and incredible transformations. Nanotechnology has tremendous and potential influence not only the way we live, but also on the way that we practice veterinary medicine and increase the safety of domestic animals, production, and income to the farmers through use of nanomaterials. The current status and advancements of nanotechnology is being used to enhance the animal growth promotion, and production. To achieve these, nanoparticles are used as alternative antimicrobial agents to overcome the usage alarming rate of antibiotics, detection of pathogenic bacteria, and also nanoparticles being used as drug delivery agents as new drug and vaccine candidates with improved characteristics and performance, diagnostic, therapeutic, feed additive, nutrient delivery, biocidal agents, reproductive aids, and finally to increase the quality of food using various kinds of functionalized nanoparticles, such as liposomes, polymeric nanoparticles, dendrimers, micellar nanoparticles, and metal nanoparticles. It seems that nanotechnology is ideal for veterinary applications in terms of cost and the availability of resources. The main focus of this review is describes some of the important current and future principal aspects of involvement of nanotechnology in Veterinary Medicine. However, we are not intended to cover the entire scenario of Veterinary Medicine, despite this review is to provide a glimpse at potential important targets of nanotechnology in the field of Veterinary Medicine. Considering the strong potential of the interaction between the nanotechnology and Veterinary Medicine, the aim of this review is to provide a concise description of the advances of nanotechnology in Veterinary Medicine, in terms of their potential application of various kinds of nanoparticles, secondly we discussed role of nanomaterials in animal health and production, and finally we discussed conclusion and future perspectives of nanotechnology in veterinary medicine.
- Published
- 2018
40. Clinical outcome comparison of polymethylmethacrylate bone cement with and without mineralized collagen modification for osteoporotic vertebral compression fractures
- Author
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Zhi-Ye Qiu, Xi Wang, Jian-Ming Kou, Xisheng Weng, Yang Yue, and Xi-Feng Zhang
- Subjects
Male ,musculoskeletal diseases ,medicine.medical_treatment ,Observational Study ,Dentistry ,02 engineering and technology ,Percutaneous vertebroplasty ,03 medical and health sciences ,0302 clinical medicine ,Biomimetic Materials ,Fractures, Compression ,medicine ,Humans ,Polymethyl Methacrylate ,Adjacent level ,Aged ,Retrospective Studies ,mineralized collagen ,vertebral compression fracture ,Aged, 80 and over ,Cement ,Pain, Postoperative ,Vertebroplasty ,PMMA bone cement ,business.industry ,Bone Cements ,technology, industry, and agriculture ,Postoperative complication ,Increased Bone Density ,General Medicine ,Middle Aged ,equipment and supplies ,021001 nanoscience & nanotechnology ,Compression (physics) ,Bone cement ,osteoporosis ,Vertebral body ,Spinal Fractures ,Female ,Collagen ,Tomography, X-Ray Computed ,0210 nano-technology ,business ,030217 neurology & neurosurgery ,Research Article - Abstract
A retrospective study of consecutive patients. The purpose of this study was to compare the clinical effect of biomimetic mineralized collagen (MC) modified polymethylmethacrylate (PMMA) bone cement and traditional PMMA bone cement for the treatment of osteoporotic vertebral compression fractures (OVCF). New fracture on adjacent level is the major postoperative complication of percutaneous vertebroplasty (PVP). The clinical incidence was 12.4% to 27.7%. The increased stiffness of the treated vertebral body caused by filling bone cement is considered as one of the main reasons. A total of 30 patients treated with traditional PMMA bone cement from June 2013 to March 2016 were selected as the traditional group, while 50 patients treated with MC modified PMMA bone cement from July 2014 to March 2016 were selected as the modified group. The 2 groups were compared by injection time of the bone cements, postoperative pain relief effects, vertebral height restoration, CT value changes of the treated vertebral bodies, and postoperative complications in the clinical observations. The surgeries were successfully completed in both groups. In the treatment of OVCF, the MC modified bone cement was able to achieve the same pain relief and vertebral height restoration effects compared to traditional bone cement during the follow-ups, although the injection time of the cement was prolonged in the operation. MC modified bone cement significantly reduced the incidence of postoperative adjacent vertebral fracture from 13.3% to 2%, and significantly increased bone density of the treated vertebral bodies. The MC modified PMMA bone cement showed good clinical outcomes and better mechanical properties than the traditional bone cements.
- Published
- 2018
41. Diethylhexyl phthalate exposure impairs follicular development and affects oocyte maturation in the mouse
- Author
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Wei Shen, Lan Li, Evanna Huynh, Jin-Mei Ma, Yanni Feng, Lian-Jun Zhang, Bo Chen, Massimo De Felici, Xi-Feng Zhang, and Qinghua Shi
- Subjects
endocrine system ,medicine.medical_specialty ,Epidemiology ,Offspring ,Health, Toxicology and Mutagenesis ,Phthalate ,Biology ,Oocyte ,Oogenesis ,chemistry.chemical_compound ,Follicle ,Endocrinology ,medicine.anatomical_structure ,Endocrine disruptor ,chemistry ,Internal medicine ,Follicular phase ,medicine ,Folliculogenesis ,Genetics (clinical) - Abstract
Diethylhexyl phthalate (DEHP) is an estrogen-like compound widely used as a commercial plasticizer and present in medical devices, tubing, food containers and packaging. It is considered an endocrine disruptor and studies on experimental animals showed that exposure to DEHP can alter the function of several organs including liver, kidneys, lungs and reproductive system, particularly the developing testes of prenatal and neonatal males. Exposure to DEHP has been proposed as a potential human health hazard. This study assessed the effects of DEHP on folliculogenesis and oocyte maturation using the mouse as the experimental model. Newborn female mice were hypodermically injected with DEHP at doses of 20 and 40 μg/kg per body weight following different exposure regimens during the weaning period. We found that DEHP altered both folliculogenesis and oocyte development. In particular, DEHP exposure significantly decreased the number of the primordial follicles at pubertal and adult age by possibly accelerating the rate of follicle recruitment dynamics, reduced and/or delayed the level of imprinted gene methylation in the oocytes and increased metaphase II spindle abnormalities in oocytes matured in vitro. Furthermore, the weight of pups and litter size of mothers exposed to DEHP were significantly lower than controls. Finally, the number of primordial follicles appeared significantly reduced also in the F1 offspring at the adult age. These results show that DEHP may have a number of adverse effects on oogenesis, especially when exposure occurs during early postnatal age, arising concerns about the exposure of human female infants and children to this compound.
- Published
- 2013
42. In Situ Synthesis and Tribological Behavior of Copper Nanopieces in Liquid Paraffin as Additive in Mobil 1 5W- 30 Lubricating Oil
- Author
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Ying Zhou, Xiao Nong Cheng, Ke Wei Li, and Xi Feng Zhang
- Subjects
Materials science ,Liquid paraffin ,Metallurgy ,General Engineering ,chemistry.chemical_element ,Polyethylene glycol ,Copper ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Transmission electron microscopy ,Nano ,Lubrication ,Lubricant ,Tribometer - Abstract
To synthesize morphology- controlled nano- copper with excellent lubrication properties as lubricant’s additive, chemical reduction synthesis method was used coalescing in situ via liquid paraffin as resolvent and modification with polyethylene glycol (PEG-2000). The as- prepared Cu nanopieces with 35.59 nm average length and 21.03 nm average width were characterized by X-ray diffraction (XRD), and transmission electron microscopy (TEM). The tribological behavior of Mobil 1 5W-30 lubricating oil with nano- Cu were measured by ball- on- disk UMT- Ⅱ tribometer and analyzed by SEM and EDS. It is found that the tribological performance of Mobil 1 5W- 30 with nano- Cu is improved, and the optimization addition is 0.4% (Cu wt) with the lowest friction coefficient.
- Published
- 2013
43. Preparation, Characterization of Cu Nanowires and their Tribological Property in Liquid Paraffin
- Author
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Jing Huang, Ke Wei Li, Ying Zhou, and Xi Feng Zhang
- Subjects
Materials science ,Tetrahydrate ,Liquid paraffin ,Metallurgy ,General Engineering ,Nanowire ,chemistry.chemical_element ,Polyethylene ,Ascorbic acid ,Microstructure ,Copper ,chemistry.chemical_compound ,Lattice constant ,chemistry ,Chemical engineering - Abstract
In order to synthesize morphology and structure-controlled nano-copper, chemical reduction synthesis method was used coalescing microwave-assisted and modification. Cu nanowires with diameters of 20 to 25nm and lengths of 1.2 to 2.1μm were yielded by reduction of copper acetate tetrahydrate of (1, 2)-propanediol system using ascorbic acid as a reductant, polyethylene sorbitan monooleate (Tween 80) as a modifier and by microwave-assisted heating. The microstructure of these nanowires has been characterized by XRD and TEM. The XRD patterns indicate that the lattice parameters of these Cu nanowires are enlarged about 1.162% while the lattice parameters of Cu nanospheres are reduced about 1.024%. The growth mechanism of Cu nanowires is also suspected. The anti--wear and reducing friction performance of liquid paraffin with either Cu nanowires or Cu nanospheres has been measured by ball--on--disk UMT--II tribometer. It is found that the tribological performance of liquid paraffin with nano-Cu is improved, and the enhancement of improvement with Cu nanowires is better than with Cu nanospheres.
- Published
- 2012
44. Expression and epigenetic dynamics of transcription regulator Lhx8 during mouse oogenesis
- Author
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Xi-Feng Zhang, Gui-Jin Liang, Jin-Mei Ma, Yanni Feng, Wei Shen, Lin-Qing Wang, Lian-Jun Zhang, Bo Pan, Bo Chen, and Lan Li
- Subjects
Blotting, Western ,LIM-Homeodomain Proteins ,Biology ,Polymerase Chain Reaction ,Epigenesis, Genetic ,Histones ,Mice ,Histone H3 ,Oogenesis ,Ovarian Follicle ,Histone methylation ,Genetics ,Animals ,Epigenetics ,Histone H3 acetylation ,3' Untranslated Regions ,DNA Primers ,Epigenomics ,Regulation of gene expression ,Base Sequence ,Gene Expression Regulation, Developmental ,Acetylation ,General Medicine ,DNA Methylation ,Immunohistochemistry ,Molecular biology ,Histone methyltransferase ,DNA methylation ,CpG Islands ,Female ,5' Untranslated Regions ,Transcription Factors - Abstract
The spatial and temporal specific activation and inhibition of numerous genes are required for successful oogenesis which is precisely regulated by germ cell-related transcription factors, and appropriate epigenetic modifications, including DNA methylation, histone modification and other mechanisms that closely regulate the functional exertion of these transcription factors. In this study, we characterized the correlation between the expression and epigenetic dynamics of Lhx8, a germ cell specific transcription factor during mouse oogenesis. Immunohistochemistry, quantitative PCR and western blots were performed to localize and quantify the expressional characteristics of Lhx8 in oocytes of 13.5 dpc (day post coitum), 17.5 dpc, 0 dpp (day post partum), 3 dpp, 7 dpp and 14 dpp. The results showed that LHX8 protein was located in the nucleus of oocytes, and increasingly expressed during primordial follicle activation. Sequencing of bisulfite-converted genomic DNAs revealed that the methylation dynamics of Lhx8-3' was highly changeable but almost no change occurred in Lhx8-5'. ChIP-QPCR analysis showed that histone H3 acetylation of Lhx8 was also increased during primordial follicle assembly and activation. In conclusion, Lhx8 expression is related with the activation of primordial follicles, which is highly correlated with the demethylation of Lhx8-3' untranslated region and the high acetylation of histone H3.
- Published
- 2012
45. [Long-term clinical studies on the treatment of lumbosacral spinal tuberculosis with CT-guided minimally invasive surgery]
- Author
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Lin, Zhang, Xi-feng, Zhang, Ke-dong, Hou, Hua-dong, Yang, Xu-gang, Jiang, and Ning-dao, Li
- Subjects
Adult ,Aged, 80 and over ,Male ,Sacrum ,Adolescent ,Lumbosacral Region ,Middle Aged ,Young Adult ,Debridement ,Child, Preschool ,Humans ,Minimally Invasive Surgical Procedures ,Female ,Tuberculosis, Spinal ,Child ,Tomography, X-Ray Computed ,Aged ,Retrospective Studies - Abstract
To evaluate the clinical effects of CT-guided percutaneous puncture and local chemotherapy for lumbosacral spinal tuberculosis.From January 2002 to March 2013, 145 patients (84 males and 61 females) with lumbosacral spinal tuberculosis underwent failed conservative treatment were treated with CT-guided minimally invasive surgery. Their clinical data were retrospectively analyzed. Among them, 143 patients were followed up, aged from 2.5 to 81 years with an average of (42.60 +/- 17.14) years. Fourteen cases (6 cases with internal fixation and 8 cases without internal fixation) recurred and 32 cases complicated with paraspinal abscess after operation. Preoperatively 1 case complicated with lower limb weakness and superficial sensation worse, 1 case with muscle strength decrease, this 2 cases were grade D of Frankle, other cases were grade E. And 1 patient underwent fenestration operation and local chemotherapy, 144 cases with CT-guided percutaneous puncture and local chemotherapy. ESR and lumbar lordosis angle of all patients were observed preoperatively and final follow-up.The mean follow-up time was 67 months (ranged, 21 to 149 months) in 143 cases, and 73 cases more than 5 years. All patients obtained clinical healing. ESR was (44.96 +/- 12.41) mm/h before operation and (7.25 +/- 3.43) mm/h at final follow-up, there was significant difference between preoperative and postoperative (t=35.06, P=0.000). Lumbar lordosis angle was (36.32 +/- 8.55) degrees before operation and (33.35 +/- 8.16) at final follow-up, there was significant difference between preoperative and postoperative (t=13.90, P=0.000).When conservative treatment fails for 3 months or more, the patients have good spinal stabilization, nerve function is more than grade D of Frankel, CT-guided percutaneous puncture and local chemotherapy can get satisfactory outcomes for lumbosacral spinal tuberculosis.
- Published
- 2016
46. Bisphenol A exposure modifies DNA methylation of imprint genes in mouse fetal germ cells
- Author
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Yanni Feng, Gui-Jin Liang, Lan Li, Wei Shen, Xi-Feng Zhang, Bo Chen, Yan-Min Feng, and Lian-Jun Zhang
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Kruppel-Like Transcription Factors ,Air Pollutants, Occupational ,Biology ,Receptor, IGF Type 2 ,Genomic Imprinting ,Mice ,DAZL ,Fetus ,Phenols ,Pregnancy ,Internal medicine ,Genetics ,medicine ,Animals ,Benzhydryl Compounds ,Molecular Biology ,Gene ,Regulation of gene expression ,urogenital system ,Gene Expression Regulation, Developmental ,Environmental Exposure ,General Medicine ,Environmental exposure ,DNA Methylation ,Germ Cells ,medicine.anatomical_structure ,Endocrinology ,Receptors, Estrogen ,embryonic structures ,DNA methylation ,CpG Islands ,Female ,Genomic imprinting ,hormones, hormone substitutes, and hormone antagonists ,Germ cell - Abstract
Bisphenol A (BPA) is an estrogenic environmental toxin widely used for the production of plastics. Human frequent exposure to this chemical has been proposed to be a potential public health risk. The objective of this study was to assess the effects of BPA on DNA methylation of imprinting genes in fetal mouse germ cell. Pregnant mice were treated with BPA at doses of 0, 40, 80 and 160 μg BPA/kg body weight/day from 0.5 day post coitum. DNA methylation of imprinting genes, Igf2r, Peg3 and H19, was decreased with the increase of BPA concentration in fetal mouse germ cells (p < 0.01).The relative mRNA levels of Nobox were lower in BPA-treated group compared to control (BPA free) in female fetal germ cells, but in male fetal germ cells, a significant higher in Nobox expression was observed in BPA-treated group compared to control. Decreased mRNA expression of specific meiotic genes including Stimulated by Stra8 and Dazl were obtained in the female fetal germ cells. In conclusion, BPA exposure can affect the DNA methylation of imprinting genes in fetal mouse germ cells.
- Published
- 2012
47. [Effects of Cordycepin on Proliferation, Apoptosis and NF-κB Signaling Pathway in A549 Cells]
- Author
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Chao, Zhang, Qin, Zhong, Xi-feng, Zhang, Dai-xi, Hu, Xue-mei, He, Quan-lun, Li, and Tao, Feng
- Subjects
Lung Neoplasms ,Deoxyadenosines ,Caspase 3 ,Cell Line, Tumor ,NF-kappa B ,Humans ,Apoptosis ,Cell Proliferation ,Signal Transduction - Abstract
To investigate the effects of Cordycepin on proliferation and apoptosis of A549 cells, and its possible mechanism of action.Human lung cancer cell line A549 cells were treated with different concentrations of Cordycepin for 24 h. MTS assay was used to detect cell proliferation. Hoechst 33258 staining and Annexin V/PI flow cytometry were used to determine the apoptosis of A549 cells. The protein expression of nuclear factor-κB p65 (NF-κB p65), BAX, BCL-2 and cleaved Caspase-3 was determined by Western blot.In a dose dependent way, Cordycepin inhibited the proliferation and promoted the apoptosis of A549 cells, increased the expression of BAX and cleaved Caspase-3, while decreased the expression of BCL-2. The Western blot results showed, Cordycepin dose-dependently inhibited the entry of NF-κB p65 to nuclear in A59 cells.Cordycepin can inhibit the proliferation and induct the apoptosis of A549 cells, the mechanism of action is achieved by inhibiting the NF-κB pathway.
- Published
- 2015
48. Experimental Study of the Factors Influencing Filtrate Loss of the High-Density Brine Drilling Fluid
- Author
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Tian Tai Li, Xi Feng Zhang, and Ming Zhang
- Subjects
Brine ,Petroleum engineering ,business.industry ,High pressure ,Drilling fluid ,Bentonite ,Fossil fuel ,General Engineering ,Environmental science ,High density ,equipment and supplies ,business - Abstract
High density brine drilling fluid has been widely applied in the high pressure and complex oil and gas fields. Effectively controlling high density brine drilling fluid loss is an important factor for reducing the reservoir damage and keeping well stability. Base on general drilling fluid formulations,the affecting factors of filtrate loss of high density brine drilling fluid were analysed through mass laboratory experiments. The results show that the main fctor was the content of caustic soda and bentonite, secondly the density and the shape of adding product. The combination of adding product is one of effective method to control the filtration property of high density brine drilling fluid. The results will provide reliable foundation for successful field application.
- Published
- 2011
49. Load Analysis of Transmitting Gear in Loading Cycles for Earthmoving Vehicle
- Author
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Ming Yao Yao, Zhenyu Wang, Xi Feng Zhang, and Jixin Wang
- Subjects
Loader ,Engineering ,Measurement method ,Non-circular gear ,business.industry ,Indirect test ,General Engineering ,Load spectrum ,Load analysis ,Fatigue testing ,Structural engineering ,business ,Automotive engineering - Abstract
Fatigue failure of gears carrying heavy loads is the leading cause of gearbox breakdown in the large-scale earthmoving machinery. The fatigue life of gear is mainly determined by loads distribution. Taking the Wheel Loader as an example, this paper introduced an indirect test system of gear load under the loose soil conditions. Then the load data in time, amplitude and frequency field were respectively analyzed. The results show that the indirect measurement method proved workable, and the load data of gear is characterized by bi-modal. Therefore, to decrease statistical errors, mixture model must be adopted to fit these bi-modal signals.
- Published
- 2011
50. Impact of diethylhexyl phthalate on gene expression and development of mammary glands of pregnant mouse
- Author
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Wei Ge, Fang-Nong Lai, Cheng-Li Dou, Jing-Cai Liu, Yong Zhao, Hong Chen, Fan Yang, Wei Shen, Lan Li, and Xi-Feng Zhang
- Subjects
G2 Phase ,endocrine system ,medicine.medical_specialty ,Histology ,Time Factors ,Cell ,Mammary gland ,Uterus ,Biology ,Female reproductive system ,Endocrine Disruptors ,Tissue Culture Techniques ,chemistry.chemical_compound ,Mice ,Mammary Glands, Animal ,Pregnancy ,Internal medicine ,Diethylhexyl Phthalate ,Gene expression ,medicine ,Animals ,RNA, Messenger ,Molecular Biology ,Cell Proliferation ,Dose-Response Relationship, Drug ,Gene Expression Profiling ,Phthalate ,Gene Expression Regulation, Developmental ,Cell Biology ,Cell cycle ,Medical Laboratory Technology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Endocrine disruptor ,Female - Abstract
The widely used diethylhexyl phthalate (DEHP) is a known endocrine disruptor that causes persistent alterations in the structure and function of female reproductive system, including ovaries, uterus and oviducts. To explore the molecular mechanism of the effect of DEHP on the development of mammary glands, we investigated the cell cycle, growth, proliferation and gene expression of mammary gland cells of pregnant mice exposed to DEHP. It was demonstrated, for the first time, that the mammary gland cells of pregnant mice treated with DEHP for 0.5–3.5 days post-coitum had increased proliferation, growth rate and number of cells in the G2/S phase. The expression of cell proliferation-related genes was significantly altered after short time and low-dose DEHP treatment of mammary gland cells in vivo and in vitro. These findings showed adverse effects of DEHP on mammary gland cells in pregnant mice.
- Published
- 2015
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