42 results on '"Xiaoping Peng"'
Search Results
2. HIF‐1α‐induced upregulated miR‐322 forms a feedback loop by targeting Smurf2 and Smad7 to activate Smad3/β‐catenin/HIF‐1α, thereby improving myocardial ischemia‐reperfusion injury
- Author
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Wei Dong, Chen Dong, Jianbing Zhu, Yaofu Zheng, Junfei Weng, Leilei Liu, Yang Ruan, Xu Fang, Jin Chen, Wenyu Liu, Xiaoping Peng, and Xuanying Chen
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Cell Biology ,General Medicine - Published
- 2023
3. Atorvastatin-induced tolerogenic dendritic cells improve cardiac remodeling by suppressing TLR-4/NF-κB activation after myocardial infarction
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Qian Wang, Zhaoyang Chen, Junjie Guo, Xiaoping Peng, Zeqi Zheng, Hang Chen, Haibo Liu, Yuanji Ma, and Jianbing Zhu
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Pharmacology ,Immunology - Abstract
Myocardial infarction (MI) caused by ischemic cardiomyocyte necrosis induces inflammatory responses that strongly affect ventricular remodeling. Tolerogenic dendritic cells (tDCs) can suppress this effect on inflammatory responses. However, the precise role of atorvastatin-induced tDCs in ventricular remodeling after MI remains unclear.To explore the effect of necrotic cardiomyocytes (SNC) and/or atorvastatin on DC function, the expression of CD40, CD80, CD86, and MHC-II was determined using flow cytometry. The protein levels of TLR-4/NF-κB-related molecules were evaluated using western blotting. The infarct area after MI was determined via 2,3,5-triphenyltetrazolium chloride staining. The TUNEL assay was employed to evaluate the apoptosis of cardiomyocytes in heart sections. Masson's trichrome method was used to determine the extent of fibrosis.Compared to the DCs co-cultured with PBS (control), cells co-cultured with Supernatant-IM or Supernatant-NH produced higher levels of inflammatory cytokines, including TNF-α, IL-1, IL-6, IL-12P40, and IL-8. This cytokine production was impaired by atorvastatin treatment. SNC treatment induced DC maturation and enhanced inflammatory cytokine secretion and oxidative stress through TLR-4/NF-κB pathway activation. Compared to that in the PBS-treated group, the left ventricular ejection fraction was significantly improved after tDC treatment. Additionally, compared to that in the PBS-treated group, tDC treatment reduced the left ventricular end-diastolic and end-systolic diameters in mice. Furthermore, treatment with tDCs improved the left ventricular systolic function, attenuated inflammatory cell infiltration, and reduced cardiomyocyte apoptosis, myocardial fibrosis, and infarct size compared to those in the control group.Adoptive transfer of atorvastatin-induced tDCs alleviated post-infarction cardiomyocyte apoptosis and myocardial fibrosis in association with decreased inflammatory cell infiltration and inhibited oxidative stress, likely by suppressing TLR-4/NF-κB activation after myocardial infarction.
- Published
- 2022
4. Type I Kounis syndrome induced by COVID-19 vaccination in China: a case report
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Yubing Deng, Zhujun Peng, and Xiaoping Peng
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Cardiology and Cardiovascular Medicine - Abstract
Background Kounis syndrome is a rare clinical condition characterized by the occurrence of an acute coronary event induced by an acute allergic episode. The ongoing pandemic of coronavirus disease 2019 (COVID-19) has contributed to an increase in the incidence of allergic reactions to a certain extent, thereby increasing the incidence of Kounis syndrome. Timely diagnosis and effective management of this disease are important in clinical practice. Case presentation We report a 43-year-old woman who developed generalized pruritus, breathlessness, paroxysmal precordial crushing pain, and dyspnea after receiving the third dose of the COVID-19 vaccine. After anti-allergic treatment and therapy for acute myocardial ischemia, her symptoms resolved with improvement in cardiac function and resolution of ST-segment changes. The prognosis was satisfactory, and the final diagnosis was type I Kounis syndrome. Conclusion This patient with type I Kounis syndrome rapidly developed acute coronary syndrome (ACS) after an acute allergic reaction to the COVID-19 vaccine. Timely diagnosis of acute allergic reaction and ACS, and targeted treatment based on the relevant guidelines are the key to successful treatment of the syndrome.
- Published
- 2023
5. miR-322 promotes the differentiation of embryonic stem cells into cardiomyocytes
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Kai Liu, Xiaoping Peng, and Liang Luo
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Genetics ,General Medicine - Abstract
Previous studies have depicted that miR-322 can regulate the function of various stem cells. However, its role and mechanism in the ability of embryonic stem cells (ESCs) to differentiate into cardiomyocytes are still unknown. Celf1 can play vital roles in stem cell differentiation and may be a potential target of miR-322 in ESCs’ differentiation. An experiment was performed on mouse ESCs transfected with lentivirus-mediated miR-322 to study the function of miR-322. RT-PCR results indicated that miR-322 could increase NKX-2.5, MLC2V, and α-MHC mRNA expression, signifying that miR-322 might promote the differentiation of ESCs towards cardiomyocytes in vitro. Western blot and immunofluorescence results likewise confirmed this conclusion. In addition, the experiment found that the knockdown of miR-322 expression could inhibit ESCs’ differentiation towards cardiomyocytes in cultured ESCs in vitro. Western blot results presented that miR-322 could suppress celf1 protein expression. Furthermore, Western blot, RT-PCR, and immunofluorescence results manifested that celf1 might inhibit ESCs’ differentiation towards cardiomyocytes in vitro. Overall, the results signified that miR-322 might promote ESCs’ differentiation towards cardiomyocytes by regulating celf1 expressions.
- Published
- 2023
6. ApoE4 associated with severe COVID-19 outcomes via downregulation of ACE2 and imbalanced RAS pathway
- Author
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Feng Chen, Yanting Chen, Qiongwei Ke, Yongxiang Wang, Zheng Gong, Xiongjin Chen, Yujie Cai, Shengnan Li, Yuanhong Sun, Xiaoping Peng, Yao Ji, Tianzhen Zhang, Wenxian Wu, Lili Cui, and Yan Wang
- Subjects
General Medicine ,General Biochemistry, Genetics and Molecular Biology - Abstract
Background Recent numerous epidemiology and clinical association studies reported that ApoE polymorphism might be associated with the risk and severity of coronavirus disease 2019 (COVID-19), and yielded inconsistent results. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection relies on its spike protein binding to angiotensin-converting enzyme 2 (ACE2) receptor expressed on host cell membranes. Methods A meta-analysis was conducted to clarify the association between ApoE polymorphism and the risk and severity of COVID-19. Multiple protein interaction assays were utilized to investigate the potential molecular link between ApoE and the SARS-CoV-2 primary receptor ACE2, ApoE and spike protein. Immunoblotting and immunofluorescence staining methods were used to access the regulatory effect of different ApoE isoform on ACE2 protein expression. Results ApoE gene polymorphism (ε4 carrier genotypes VS non-ε4 carrier genotypes) is associated with the increased risk (P = 0.0003, OR = 1.44, 95% CI 1.18–1.76) and progression (P Conclusions ApoE4 increases SARS-CoV-2 infectivity in a manner that may not depend on differential interactions with the spike protein or ACE2. Instead, ApoE4 downregulates ACE2 protein expression and subsequently the dysregulation of renin–angiotensin system (RAS) may provide explanation by which ApoE4 exacerbates COVID-19 disease.
- Published
- 2023
7. Single-Cell Sequencing Revealed Pivotal Genes Related to Prognosis of Myocardial Infarction Patients
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Jiamin Zhou, Tong Wen, Qing Li, Zhixin Chen, Xiaoping Peng, Chunying Wei, Yunfeng Wei, Jingtian Peng, and Wei Zhang
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CD4-Positive T-Lymphocytes ,Genetic Markers ,Stochastic Processes ,Article Subject ,General Immunology and Microbiology ,Gene Expression Profiling ,Applied Mathematics ,Myocardial Infarction ,Computational Biology ,General Medicine ,Prognosis ,General Biochemistry, Genetics and Molecular Biology ,Gene Ontology ,Modeling and Simulation ,Humans ,Gene Regulatory Networks ,Mast Cells ,RNA-Seq ,Chemokines ,Single-Cell Analysis ,Immunologic Memory - Abstract
Objectives. Myocardial infarction (MI) is a common cardiovascular disease. Histopathology is a main molecular characteristic of MI, but often, differences between various cell subsets have been neglected. Under this premise, MI-related molecular biomarkers were screened using single-cell sequencing. Methods. This work examined immune cell abundance in normal and MI samples from GSE109048 and determined differences in the activated mast cells and activated CD4 memory T cells, resting mast cells. Weighted gene coexpression network analysis (WGCNA) demonstrated that activated CD4 memory T cells were the most closely related to the turquoise module, and 10 hub genes were screened. Single-cell sequencing data (scRNA-seq) of MI were examined. We used t -distributed stochastic neighbor embedding ( t -SNE) for cell clustering. Results. We obtained 8 cell subpopulations, each of which had different marker genes. 7 out of the 10 hub genes were detected by single-cell sequencing analysis. The expression quantity and proportion of the 7 genes were different in 8 cell clusters. Conclusion. In general, our study revealed the immune characteristics and determined 7 prognostic markers for MI at the single-cell level, providing a new understanding of the molecular characteristics and mechanism of MI.
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- 2022
8. Experimental Study of Hepatic Artery Infusion of Vascular Endothelial Growth Factor Receptor (VEGFR)-2As2O3 Nanospheres for Targeted Therapy of Implanted Hep G2 Liver Cancer in Rats
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Yunzhong Liao, Xiaoping Peng, and Guangbin Jiang
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Biomedical Engineering ,Medicine (miscellaneous) ,Bioengineering ,Biotechnology - Abstract
This study assesses the effect of VEGFR-2/As2O3 invisible nanospheres on treating liver cancer. The following groups were set: Group I: blank control group (hepatic artery perfusion 0.9% saline 0.5 ml), group II: VEGFR-2/As2O3 nanospheres injection via tail vein, group III: hepatic artery perfusion of VEGFR-2/As2O3 nanospheres. The effect of hepatic artery infusion of VEGFR-2/As2O3 nanospheres on cell proliferation, apoptosis and colony forming ability was evaluated by MTT method, flow cytometry and colony formation experiment. Tumor xenotransplantation was established to observe the effect of hepatic artery infusion of VEGFR-2/As2O3 nanospheres on liver cancer. The in vivo and in vitro experiments both confirmed that hepatic artery perfusion of VEGFR-2/As2O3 nanospheres can inhibit the proliferation of liver cancer cells, promote cell apoptosis and inhibit cell migration, thereby enhancing the therapeutic effect. The hepatic artery perfusion of VEGFR-2As2O3 nanospheres may be used as a targeted research and development direction for the treatment of liver cancer, providing a new and efficient targeted drug for the interventionaltreatment of liver cancer.
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- 2022
9. Qili Qiangxin as an Adjuvant Treatment with Inotrope for Advanced Heart Failure: Retrospective Analysis
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Jinghai Hua, Ling Yu, Wenjun Xiong, Jianbing Zhu, Zhiming Gao, Yaofu Zheng, Dasong Yi, Xiaoping Peng, and Zeqi Zheng
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Pharmacology - Published
- 2022
10. ApoE4 causes severe COVID-19 outcomes via downregulation of ACE2
- Author
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Feng Chen, Yanting Chen, Qiongwei Ke, Yongxiang Wang, Zheng Gong, Xiongjin Chen, Yujie Cai, Shengnan Li, Yuanhong Sun, Xiaoping Peng, Yao Ji, Tianzhen Zhang, Wenxian Wu, Lili Cui, and Yan wang
- Abstract
Background Recent numerous epidemiology and clinical association studies reported that ApoE polymorphism may associate with the risk and severity of coronavirus disease 2019 (COVID-19), and yielded inconsistent results. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection relies on its spike protein binding to angiotensin-converting enzyme 2 (ACE2) receptor expressed on host cell membranes. Methods A meta-analysis was conducted to clarify the association between ApoE polymorphism and the risk and severity of COVID-19. Multiple protein interaction assays were utilized to investigate the potential molecular link between ApoE and spike protein and between ApoE and also the SARS-CoV-2 primary receptor ACE2. Immunoblotting and immunofluorescence staining methods were used to access the regulatory effect of different ApoE isoform on ACE2 protein expression. Results ApoE gene polymorphism (ε4 carries genotypes VS non-ε4 carries genotypes) is associated with the increased risk (P = 0.0003, OR = 1.44, 95% CI: 1.18–1.76) and progression (P in vitro and in vivo and subsequently decreases the conversion of Ang II to Ang 1–7. Conclusions ApoE4 increases SARS-CoV-2 infectivity in a manner that may not depend on differential interactions with the spike protein or ACE2. Instead, ApoE4 downregulates ACE2 protein expression and subsequently the dysregulation of renin–angiotensin system (RAS) may provide explanation by which ApoE4 exacerbates COVID-19 disease.
- Published
- 2022
11. Structures and Biological Activities of Secondary Metabolites from
- Author
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Rui, Guo, Gang, Li, Zhao, Zhang, and Xiaoping, Peng
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Trichoderma ,Hypocreales - Abstract
The biocontrol fungus
- Published
- 2022
12. ApoE4 causes severe COVID-19 outcomes via downregulation of ACE2
- Author
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Feng Chen, Yanting Chen, Qiongwei Ke, Yongxiang Wang, Xiongjin Chen, Xiaoping Peng, Yujie Cai, Shennan Li, Yuanhong Sun, Yao Ji, Yuling Jiang, Wenxian Wu, Yan Wang, and Lili Cui
- Abstract
The coronavirus disease 2019 (COVID-19) pandemic is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); host cell entry by this virus relies on the interaction between the receptor-binding domain (RBD) of its spike glycoprotein and the angiotensin-converting enzyme 2 (ACE2) receptor on cell membranes. In addition to serving as a receptor for SARS-CoV-2, ACE2 was originally discovered as a protective factor in the renin–angiotensin system (RAS) that catalyses the degradation of angiotensin II (Ang II) to Ang 1-7, which is involved in multiple organ pathology. Recent genetic and clinical studies reported that ApoE4 expression is associated with increased susceptibility to SARS-CoV-2 infection and the development of severe COVID-19, but the underlying mechanism is currently unclear. In the present study, by using immunofluorescence staining, molecular dynamics simulations, proximity ligation assay (PLA) and coimmunoprecipitation (Co-IP) combined with a biolayer interferometry (BLI) assay, we found that ApoE interacts with both the spike protein and ACE2 but does not show obvious isoform-dependent binding effects. These data suggest that ApoE4 increases SARS-CoV-2 infectivity in a manner that may not depend on differential interactions with the spike protein or ACE2. Importantly, further immunoblotting and immunofluorescence staining results showed that ApoE4 significantly downregulates ACE2 protein expression in vitro and in vivo and subsequently decreases the conversion of Ang II to Ang 1-7, which could worsen tissue lesions; these findings provide a possible explain by which ApoE4 exacerbates COVID-19 disease.
- Published
- 2022
13. HIF-1α-induced up-regulated miR-322 forms a feedback loop by targeting Smurf2 and Smad7 to activate Smad3/β-catenin/HIF-1α, thereby improving myocardial ischemia-reperfusion injury
- Author
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Wei Dong, Chen Dong, Jianbing Zhu, Yaofu Zheng, Junfei Weng, Leilei Liu, Yang Ruan, Xu Fang, Jin Chen, Wenyu Liu, Xiaoping Peng, and Xuanying Chen
- Abstract
Background: Myocardial ischemia/reperfusion injury (MIRI) is a major cause of heart failure after myocardial infarction. It has been reported that miR-322 is involved in MIRI progression, while the molecular mechanism of miR-322 in regulating MIRI progression needs to be further probed.Methods: MIRI cell model was established by oxygen‐glucose deprivation/reoxygenation (OGD/R). Cell viability was assessed using MTS assay. Flow cytometry and TUNEL staining were employed to analyze cell apoptosis. In addition, the interactions between miR-322, Smad7/Smurf2, HIF-1α and β-catenin were verified by dual-luciferase reporter gene assay.Results: Our results displayed that miR-322 was significantly downregulated in OGD/R-treated H9c2 cells, and its overexpression resulted in increased cell viability and reduced the apoptosis. Smurf2 and Smad7 were identified as the direct targets of miR-322. Smad7 knockdown or Smurf2 knockdown increased OGD/R-treated H9c2 cell viability and suppressed the apoptosis. Meanwhile, miR-322 mimics abolished the mitigating effect of Smad7 or Smurf2 overexpression on MIRI. In addition, the Smad3/β-catenin pathway was identified as the downstream pathway of Smurf2/Smad7. Moreover, it was found that HIF-1α interacted with the miR-322 promoter, and β-catenin interacted with the HIF-1α promoter to form a loop.Conclusion: HIF-1α-induced up-regulated miR-322 activated the Smad3/β-catenin pathway by targeting Smurf2 and Smad7 to improve MIRI; meanwhile, β-catenin/ HIF-1α formed a positive feedback loop to continuously improve MIRI.
- Published
- 2022
14. High Salt Elicits Brain Inflammation and Cognitive Dysfunction, Accompanied by Alternations in the Gut Microbiota and Decreased SCFA Production
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Li Hu, Shaoping Zhu, Xiaoping Peng, Kanglan Li, Wanjuan Peng, Yu Zhong, Chenyao Kang, Xingxing Cao, Zhou Liu, and Bin Zhao
- Abstract
Background: Excessive salt intake is considered as an important risk factor for cognitive impairment, which might be the consequence of imbalanced intestinal homeostasis. Objective: To investigate the effects of dietary salt on the gut microbiota and cognitive performance and the underlying mechanisms. Methods: Adult female C57BL/6 mice were maintained on either normal chow (control group, CON) or sodium-rich chow containing 8% NaCl (high-salt diet, HSD) for 8 weeks. Spatial learning and memory ability, short-chain fatty acids (SCFAs) concentrations, gut bacterial flora composition, blood-brain barrier permeability, and proinflammatory cytokine levels and apoptosis in the brain were evaluated. Results: The mice fed a HSD for 8 weeks displayed impaired learning and memory abilities. HSD significantly reduced the proportions of Bacteroidetes (S24-7 and Alloprevotella) and Proteobacteria and increased that of Firmicutes (Lachnospiraceae and Ruminococcaceae). SCFA concentrations decreased in the absolute concentrations of acetate, propionate, and butyrate in the fecal samples from the HSD-fed mice. The HSD induced both BBB dysfunction and microglial activation in the mouse brain, and increased the IL-1β, IL-6, and TNF-α expression levels in the cortex. More importantly, the degree of apoptosis was higher in the cortex and hippocampus region of mice fed the HSD, and this effect was accompanied by significantly higher expression of cleaved caspase-3, caspase-3, and caspase-1. Conclusion: The HSD directly causes cognitive dysfunction in mice by eliciting an inflammatory environment and triggering apoptosis in the brain, and these effects are accompanied by gut dysbiosis, particularly reduced SCFA production.
- Published
- 2022
15. A Series of New Pyrrole Alkaloids with ALR2 Inhibitory Activities from the Sponge
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Qi, Wang, Chunhua, Gao, Zhun, Wei, Xiaowen, Tang, Lixia, Ji, Xiangchao, Luo, Xiaoping, Peng, Gang, Li, and Hongxiang, Lou
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Alkaloids ,Magnetic Resonance Spectroscopy ,Molecular Structure ,Animals ,Pyrroles ,Porifera - Abstract
Twelve new and four known alkaloids including five different structural scaffolds were isolated from the sponge
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- 2022
16. Bivalirudin Presents a Favorable Safety Profile Regarding Adverse Drug Reactions, Thrombocytopenia, and Bleeding in Chinese Patients With High Bleeding Risk Undergoing Percutaneous Coronary Intervention: A Prospective, Multi-Center, Intensive Monitoring Study
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Xiaoping Peng, Zhenyong Li, Dunheng Li, Zhongyin Li, Zhaohua Lu, Caidong Luo, and Zheng Ji
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Cardiology and Cardiovascular Medicine - Abstract
BackgroundThis study aimed to comprehensively explore the occurrence and risk factors for adverse events (AEs) and adverse drug reactions (ADRs) (especially for thrombocytopenia and bleeding) in Chinese patients with high bleeding risk (older adults, or complicated with diabetes mellitus or renal function impairment) undergoing percutaneous coronary intervention (PCI) with bivalirudin as an anticoagulant.MethodsA total of 1,226 patients with high bleeding risk who received PCI with bivalirudin as an anticoagulant from 27 Chinese medical centers were enrolled in this prospective, multi-center, intensive monitoring study. AEs, ADRs, thrombocytopenia, and bleeding were collected from admission to 72 h post-bivalirudin administration; subsequently, patients were followed up on the 30th day with the safety data collected as well.ResultsAdverse events were observed in 198 (16.2) patients, among which severe AEs occurred in 16 (1.3%) patients. Meanwhile, bivalirudin-related ADRs were reported in 66 (5.4%) patients, among which 5 (0.4%) patients experienced bivalirudin-related severe ADRs. Besides, thrombocytopenia and bleeding occurred in 45 (3.7%) and 19 (1.5%) patients, respectively. The subsequent multivariate logistic analysis revealed that age >75 years [p = 0.017, odds ratio (OR) = 1.856] and spontaneous coronary artery dissection (SCAD) (p = 0.030, OR = 2.022) were independently related to higher ADR risk; SCAD (p = 0.017, OR = 2.426) was independently correlated with higher thrombocytopenia risk, while radial artery access (p = 0.015, OR = 0.352) was independently correlated with lower thrombocytopenia risk; and the administration of bivalirudin preoperatively or intraoperatively (p = 0.013, OR = 5.097) was independently associated with higher bleeding risk.ConclusionBivalirudin presents a favorable safety profile regarding ADRs, thrombocytopenia, and bleeding in Chinese patients with high bleeding risk undergoing PCI.
- Published
- 2022
17. Construction of Novel Gene Signature-Based Predictive Model for the Diagnosis of Acute Myocardial Infarction by Combining Random Forest With Artificial Neural Network
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Yanze Wu, Hui Chen, Lei Li, Liuping Zhang, Kai Dai, Tong Wen, Jingtian Peng, Xiaoping Peng, Zeqi Zheng, Ting Jiang, and Wenjun Xiong
- Subjects
Cardiology and Cardiovascular Medicine - Abstract
BackgroundAcute myocardial infarction (AMI) is one of the most common causes of mortality around the world. Early diagnosis of AMI contributes to improving prognosis. In our study, we aimed to construct a novel predictive model for the diagnosis of AMI using an artificial neural network (ANN), and we verified its diagnostic value via constructing the receiver operating characteristic (ROC).MethodsWe downloaded three publicly available datasets (training sets GSE48060, GSE60993, and GSE66360) from Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were identified between 87 AMI and 78 control samples. We applied the random forest (RF) and ANN algorithms to further identify novel gene signatures and construct a model to predict the possibility of AMI. Besides, the diagnostic value of our model was further validated in the validation sets GSE61144 (7 AMI patients and 10 controls), GSE34198 (49 AMI patients and 48 controls), and GSE97320 (3 AMI patients and 3 controls).ResultsA total of 71 DEGs were identified, of which 68 were upregulated and 3 were downregulated. Firstly, 11 key genes in 71 DEGs were screened with RF classifier for the classification of AMI and control samples. Then, we calculated the weight of each key gene using ANN. Furthermore, the diagnostic model was constructed and named neuralAMI, with significant predictive power (area under the curve [AUC] = 0.980). Finally, our model was validated with the independent datasets GSE61144 (AUC = 0.900), GSE34198 (AUC = 0.882), and GSE97320 (AUC = 1.00).ConclusionMachine learning was used to develop a reliable predictive model for the diagnosis of AMI. The results of our study provide potential gene biomarkers for early disease screening.
- Published
- 2022
18. PINK1/Parkin-mediated mitophagy in cardiovascular disease: From pathogenesis to novel therapy
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Yanze Wu, Ting Jiang, Jinghai Hua, Zhiping Xiong, Kai Dai, Hui Chen, Lei Li, Jingtian Peng, Xiaoping Peng, Zeqi Zheng, and Wenjun Xiong
- Subjects
Cardiovascular Diseases ,Ubiquitin-Protein Ligases ,Mitophagy ,Humans ,Cardiology and Cardiovascular Medicine ,Protein Kinases ,Mitochondria - Abstract
Cardiovascular disease(CVD)is one of the predominant causes of death and morbidity. Mitochondria play a key role in maintaining cardiac energy metabolism. However, mitochondrial dysfunction leads to excessive production of ROS, resulting in oxidative damage to cardiomyocytes and contributing to a variety of cardiovascular diseases. In such a case, the clearance of impaired mitochondria is necessary. Currently, most studies have indicated an essential role for mitophagy in maintaining cardiac homeostasis and regulating CVD-related metabolic transition. Recent studies have implicated that PTEN-induced putative kinase 1 (PINK1)/Parkin-mediated mitophagy has been implicated in maintaining cardiomyocyte homeostasis. Here, we discuss the physiological and pathological roles of PINK1/Parkin-mediated mitophagy in the cardiovascular system, as well as potential therapeutic strategies based on PINK1/Parkin-mediated mitophagy modulation, which are of great significance for the prevention and treatment of cardiovascular diseases.
- Published
- 2022
19. Exosomal <scp>LINC01005</scp> derived from oxidized low‐density lipoprotein‐treated endothelial cells regulates vascular smooth muscle cell phenotypic switch
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Zhiming Gao, Jiamin Zhou, Dasong Yi, Yaofu Zheng, Xiaoping Peng, Xiaoliang Hu, Zhiliang Zhang, Guoqiu Ying, and Tong Wen
- Subjects
0301 basic medicine ,Vascular smooth muscle ,Myocytes, Smooth Muscle ,Clinical Biochemistry ,Cell ,Kruppel-Like Transcription Factors ,Exosomes ,Biochemistry ,Muscle, Smooth, Vascular ,Umbilical vein ,Kruppel-Like Factor 4 ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,Downregulation and upregulation ,Cell Movement ,Human Umbilical Vein Endothelial Cells ,medicine ,Humans ,Cell Proliferation ,medicine.diagnostic_test ,Chemistry ,Cell growth ,Endothelial Cells ,General Medicine ,musculoskeletal system ,Microvesicles ,Cell biology ,Lipoproteins, LDL ,MicroRNAs ,Phenotype ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,KLF4 ,030220 oncology & carcinogenesis ,cardiovascular system ,Molecular Medicine ,RNA, Long Noncoding - Abstract
Phenotype switch of vascular smooth muscle cells (VSMCs) plays an important role in the development of atherosclerosis (AS). Endothelial cells can regulate VSMC phenotypic switch by secreting exosomes, crucial mediators of intracellular communication. This study aimed to determine whether exosomal LINC01005 from oxidized low-density lipoprotein (ox-LDL)-treated human umbilical vein endothelial cells (HUVECs) plays a role in regulating VSMC phenotypic switch and to validate the underlying molecular mechanism. Exosomes were extracted from ox-LDL-treated HUVECs (ox-LDL-Exo) and then administered into VSMCs. VSMC phenotypic switch was assessed by determining VSMC phenotypic markers using western blot. VSMC cell proliferation and migration were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and wound healing assay, respectively. The interaction between miR-128-3p and LINC01005 or Krüppel-like factor 4 (KLF4) was analyzed by luciferase reporter assay. ox-LDL-Exo contained high expression of LINC01005. Inhibition of LINC01005 expression in ox-LDL-Exo abrogated the ox-LDL-Exo-induced VSMC phenotypic switch, proliferation, and migration. Furthermore, LINC01005 acted as a sponge of miR-128-3p to upregulate KLF4 expression. Moreover, miR-128-3p overexpression and KLF4 silencing in VSMCs attenuated the ox-LDL-Exo-induced VSMC phenotypic switch, proliferation, and migration. Collectively, exosomal LINC01005 from ox-LDL-treated HUVECs promotes VSMC phenotype switch, proliferation, and migration by regulating the miR-128-3p/KLF4 axis.
- Published
- 2020
20. Structures and Biological Activities of Secondary Metabolites from Trichoderma harzianum
- Author
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Rui Guo, Gang Li, Zhao Zhang, and Xiaoping Peng
- Subjects
Drug Discovery ,Pharmaceutical Science ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) - Abstract
The biocontrol fungus Trichoderma harzianum, from both marine and terrestrial environments, has attracted considerable attention. T. harzianum has a tremendous potential to produce a variety of bioactive secondary metabolites (SMs), which are an important source of new herbicides and antibiotics. This review prioritizes the SMs of T. harzianum from 1988 to June 2022, and their relevant biological activities. Marine-derived SMs, especially terpenoids, polyketides, and macrolides compounds, occupy a significant proportion of natural products from T. harzianum, deserving more of our attention.
- Published
- 2022
21. New Xanthones with Antiagricultural Fungal Pathogen Activities from the Endophytic Fungus
- Author
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Xiaoping, Peng, Fusheng, Sun, Gang, Li, Cong, Wang, Yuhan, Zhang, Changzheng, Wu, Chunyang, Zhang, Yong, Sun, Siyi, Wu, Yuxiang, Zhang, Hui, Zong, Rui, Guo, and Hongxiang, Lou
- Subjects
Ascomycota ,Molecular Structure ,Circular Dichroism ,Xanthones ,Colletotrichum - Abstract
Six new xanthone dimers, diaporxanthones A-F (
- Published
- 2021
22. Influence of Prosocial Motivation on Employee Creativity: The Moderating Role of Regulatory Focus and the Mediating Role of Knowledge Sharing
- Author
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Xizhou Tian, Xiqiang Peng, and Xiaoping Peng
- Subjects
prevention focus ,Mechanism (biology) ,media_common.quotation_subject ,Regulatory focus theory ,Context (language use) ,employee creativity ,Creativity ,prosocial motivation ,Knowledge sharing ,BF1-990 ,Information processing theory ,Promotion (rank) ,Prosocial behavior ,promotion focus ,Psychology ,knowledge sharing ,Social psychology ,General Psychology ,media_common ,Original Research - Abstract
Stimulating and improving the creativity of employees are both theoretically and practically important. The relationship between prosocial motivation and creativity has gradually gained attention in recent years; however, in the context of controlling for intrinsic motivation, the influence process and results between the two are not yet clear. Based on the motivated information processing model, componential theory of creativity, and regulatory focus theory, this study analyzed the mediating role of knowledge sharing and the moderating role of regulatory focus in the relationship between prosocial motivation and the creativity of employees. For this, we used the PROCESS program and the bootstrap method to test the theoretical hypotheses. Consequently, a survey of 320 Chinese employees revealed that, under the condition of controlling for intrinsic motivation, the prosocial motivation of employees was positively related to creativity and partially mediated by knowledge sharing. Furthermore, regulatory focus negatively moderated the correlation between prosocial motivation and knowledge sharing. Specifically, we found that the higher the prevention focus was, the weaker the effect prosocial motivation had on knowledge sharing. Contrary to the hypothesis, promotion focus also played a negative moderating role. Thus, the results revealed the mechanism and boundary conditions of prosocial motivation on creativity. This study expands the research on prosocial motivation and provides guidance on how managers can enhance the creativity of their employees.
- Published
- 2021
23. Ubiquitination of Rheb governs growth factor-induced mTORC1 activation
- Author
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Lei Chen, Lu Deng, Xiaoping Peng, Fei Yu, Xinbo Wang, Jiali Jin, Lin Ding, Yanming Wang, Weijuan Pan, Hongqi Teng, Ping Wang, Linlin Zhao, Xin Ge, Yan Xu, Li Li, and Lujian Liao
- Subjects
Male ,Ubiquitin-Protein Ligases ,Mice, Nude ,mTORC1 ,Mechanistic Target of Rapamycin Complex 1 ,Biology ,Transfection ,Article ,Gene Knockout Techniques ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Ubiquitin ,Animals ,Humans ,Molecular Biology ,Mechanistic target of rapamycin ,030304 developmental biology ,Mice, Knockout ,Sirolimus ,0303 health sciences ,HEK 293 cells ,Ubiquitination ,Cell Biology ,HCT116 Cells ,Xenograft Model Antitumor Assays ,Tumor Burden ,Cell biology ,Ubiquitin ligase ,HEK293 Cells ,biology.protein ,Intercellular Signaling Peptides and Proteins ,Phosphorylation ,Ras Homolog Enriched in Brain Protein ,Ubiquitin-Specific Proteases ,biological phenomena, cell phenomena, and immunity ,Colorectal Neoplasms ,Lysosomes ,030217 neurology & neurosurgery ,Deubiquitination ,RHEB - Abstract
Mechanistic target of rapamycin mTOR complex 1 (mTORC1) plays a key role in the integration of various environmental signals to regulate cell growth and metabolism. mTORC1 is recruited to the lysosome where it is activated by its interaction with GTP-bound Rheb GTPase. However, the regulatory mechanism of Rheb activity remains largely unknown. Here, we show that ubiquitination governs the nucleotide-bound status of Rheb. Lysosome-anchored E3 ligase RNF152 catalyzes Rheb ubiquitination and promotes its binding to the TSC complex. EGF enhances the deubiquitination of Rheb through AKT-dependent USP4 phosphorylation, leading to the release of Rheb from the TSC complex. Functionally, ubiquitination of Rheb is linked to mTORC1-mediated signaling and consequently regulates tumor growth. Thus, we propose a mechanistic model whereby Rheb–mediated mTORC1 activation is dictated by a dynamic opposing act between Rheb ubiquitination and deubiquitination that are catalyzed by RNF152 and USP4 respectively.
- Published
- 2018
24. High Salt Elicits Brain Inflammation and Cognitive Dysfunction, Accompanied by Alternations in the Gut Microbiota and Decreased SCFA Production
- Author
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Xingxing Cao, Xiaoping Peng, Wanjuan Peng, Shaoping Zhu, Bin Zhao, Kanglan Li, Chenyao Kang, Zhou Liu, Li Hu, and Yu Zhong
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Hippocampus ,Inflammation ,Butyrate ,Gut flora ,Blood–brain barrier ,Proinflammatory cytokine ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Cognitive Dysfunction ,Salt intake ,Sodium Chloride, Dietary ,Maze Learning ,biology ,Chemistry ,General Neuroscience ,Lachnospiraceae ,Brain ,General Medicine ,biology.organism_classification ,Fatty Acids, Volatile ,Gastrointestinal Microbiome ,Mice, Inbred C57BL ,Psychiatry and Mental health ,Clinical Psychology ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Blood-Brain Barrier ,Female ,Geriatrics and Gerontology ,medicine.symptom ,Inflammation Mediators ,030217 neurology & neurosurgery - Abstract
Background: Excessive salt intake is considered as an important risk factor for cognitive impairment, which might be the consequence of imbalanced intestinal homeostasis. Objective: To investigate the effects of dietary salt on the gut microbiota and cognitive performance and the underlying mechanisms. Methods: Adult female C57BL/6 mice were maintained on either normal chow (control group, CON) or sodium-rich chow containing 8% NaCl (high-salt diet, HSD) for 8 weeks. Spatial learning and memory ability, short-chain fatty acids (SCFAs) concentrations, gut bacterial flora composition, blood-brain barrier permeability, and proinflammatory cytokine levels and apoptosis in the brain were evaluated. Results: The mice fed a HSD for 8 weeks displayed impaired learning and memory abilities. HSD significantly reduced the proportions of Bacteroidetes (S24-7 and Alloprevotella) and Proteobacteria and increased that of Firmicutes (Lachnospiraceae and Ruminococcaceae). SCFA concentrations decreased in the absolute concentrations of acetate, propionate, and butyrate in the fecal samples from the HSD-fed mice. The HSD induced both BBB dysfunction and microglial activation in the mouse brain, and increased the IL-1β, IL-6, and TNF-α expression levels in the cortex. More importantly, the degree of apoptosis was higher in the cortex and hippocampus region of mice fed the HSD, and this effect was accompanied by significantly higher expression of cleaved caspase-3, caspase-3, and caspase-1. Conclusion: The HSD directly causes cognitive dysfunction in mice by eliciting an inflammatory environment and triggering apoptosis in the brain, and these effects are accompanied by gut dysbiosis, particularly reduced SCFA production.
- Published
- 2020
25. Weighted correlation network bioinformatics uncovers a key molecular biosignature driving the left-sided heart failure
- Author
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Jingtian Peng, Tong Wen, Chunying Wei, Wei Zhang, Ran Yin, Jiamin Zhou, Xiaoping Peng, Yunfeng Wei, Hongmei Qi, Dasong Yi, Zhiliang Zhang, and Zeqi Zheng
- Subjects
Cardiomyopathy, Dilated ,0301 basic medicine ,lcsh:Internal medicine ,Candidate gene ,lcsh:QH426-470 ,Ischemic heart disease ,Dilated cardiomyopathy ,Computational biology ,030204 cardiovascular system & hematology ,Extracellular matrix structural constituent ,03 medical and health sciences ,0302 clinical medicine ,Weighted gene co-expression network analysis ,Gene expression ,Genetics ,Humans ,Gene Regulatory Networks ,lcsh:RC31-1245 ,Transcription factor ,Gene ,Genetics (clinical) ,Heart Failure ,biology ,Gene Expression Profiling ,Computational Biology ,Transforming growth factor beta ,Prognosis ,Human genetics ,lcsh:Genetics ,030104 developmental biology ,biology.protein ,DNA microarray ,Biomarkers ,Research Article - Abstract
BackgroundLeft-sided heart failure (HF) is documented as a key prognostic factor in HF. However, the relative molecular mechanisms underlying left-sided HF is unknown. The purpose of this study is to unearth significant modules, pivotal genes and candidate regulatory components governing the progression of left-sided HF by bioinformatical analysis.MethodsA total of 319 samples in GSE57345 dataset were used for weighted gene correlation network analysis (WGCNA). ClusterProfiler package in R was used to conduct functional enrichment for genes uncovered from the modules of interest. Regulatory networks of genes were built using Cytoscape while Enrichr database was used for identification of transcription factors (TFs). The MCODE plugin was used for identifying hub genes in the modules of interest and their validation was performed based on GSE1869 dataset.ResultsA total of six significant modules were identified. Notably, the blue module was confirmed as the most crucially associated with left-sided HF, ischemic heart disease (ISCH) and dilated cardiomyopathy (CMP). Functional enrichment conveyed that genes belonging to this module were mainly those driving the extracellular matrix-associated processes such as extracellular matrix structural constituent and collagen binding. A total of seven transcriptional factors, including Suppressor of Zeste 12 Protein Homolog (SUZ12) and nuclear factor erythroid 2 like 2 (NFE2L2), adrenergic receptor (AR), were identified as possible regulators of coexpression genes identified in the blue module. A total of three key genes (OGN, HTRA1 and MXRA5) were retained after validation of their prognostic value in left-sided HF. The results of functional enrichment confirmed that these key genes were primarily involved in response to transforming growth factor beta and extracellular matrix.ConclusionWe uncovered a candidate gene signature correlated with HF, ISCH and CMP in the left ventricle, which may help provide better prognosis and therapeutic decisions and in HF, ISCH and CMP patients.
- Published
- 2020
26. Regulatory T cells and T helper 17 cells in viral infection
- Author
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Zhikai Wan, Xiaoping Peng, Yao Liu, Wei Zou, Yuhan Lai, Yuan Luo, and Zhifeng Zhou
- Subjects
0301 basic medicine ,Cellular differentiation ,Hepatitis C virus ,Immunology ,chemical and pharmacologic phenomena ,Cell Communication ,Biology ,Lymphocyte Activation ,medicine.disease_cause ,T-Lymphocytes, Regulatory ,Virus ,Immunomodulation ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,T-Lymphocyte Subsets ,medicine ,Animals ,Humans ,Lymphocyte Count ,Central element ,Hepatitis B virus ,General Medicine ,030104 developmental biology ,Virus Diseases ,Host-Pathogen Interactions ,Th17 Cells ,Signal transduction ,Homeostasis ,Signal Transduction ,030215 immunology - Abstract
CD4+ T cells are the central element of the adaptive immune responses and protect the body from a variety of pathogens. Starting from naive cells, CD4+ T cells can differentiate into various effector cell subsets with specialized functions including T helper (Th) 1, Th2, Th17, regulatory T (Treg) and T follicular helper (Tfh) cells. Among them, Tregs and Th17 cells show a strong plasticity allowing the functional adaptation to various physiological and pathological environments during immune responses. Although they are derived from the same precursor cells and their differentiation pathways are interrelated, the terminally differentiated cells have totally opposite functions. Studies have shown that Tregs and Th17 cells have rather complex interplays in viral infection: Th17 cells may contribute to immune activation and disease progression while Tregs may inhibit this process and play a key role in the maintenance of immune homoeostasis, possibly at the cost of compromised viral control. In this review, we take respiratory syncytial virus (RSV), hepatitis B virus (HBV)/hepatitis C virus (HCV) and human immunodeficiency virus (HIV) infections as examples to discuss these interplays and their impacts on disease progression in viral infection.
- Published
- 2020
27. Exosome-Derived circITGB1 Regulates Dendritic Cell Maturation and Cardiac Inflammation via miR-342-3p/NFAM1
- Author
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Jianbing Zhu, Zhaoyang Chen, Qian Wang, Junjie Guo, Leilei Ma, Hongtao Shi, Kang Yao, Shuning Zhang, Zhenzhong Zheng, Aiping Le, Xiaoping Peng, and Zeqi Zheng
- Published
- 2020
28. OTUB1 protein suppresses mTOR complex 1 (mTORC1) activity by deubiquitinating the mTORC1 inhibitor DEPTOR
- Author
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Weijuan Pan, Chenchen Jiao, Lu Deng, Ping Wang, Xiao Tan, Xinbo Wang, Yue Yu, Lei Chen, Xin Ge, Linlin Zhao, Xiaoping Peng, and Guo-li Gao
- Subjects
0301 basic medicine ,mTORC1 ,Mechanistic Target of Rapamycin Complex 1 ,DEPTOR ,Biochemistry ,mTORC2 ,Deubiquitinating enzyme ,03 medical and health sciences ,0302 clinical medicine ,Protein Domains ,Autophagy ,Humans ,Molecular Biology ,Mechanistic target of rapamycin ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Deubiquitinating Enzymes ,biology ,Protein Stability ,Chemistry ,Intracellular Signaling Peptides and Proteins ,Ubiquitination ,Cell Biology ,Cell biology ,Cysteine Endopeptidases ,030104 developmental biology ,OTUB1 ,030220 oncology & carcinogenesis ,biology.protein ,HeLa Cells ,Deubiquitination - Abstract
Mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) integrates various environmental signals to regulate cell growth and metabolism. DEPTOR, also termed DEPDC6, is an endogenous inhibitor of mTORC1 and mTORC2 activities. The abundance of DEPTOR centrally orchestrates the mTOR signaling network. However, the mechanisms by which DEPTOR stability is regulated are still elusive. Here, we report that OTU domain–containing ubiquitin aldehyde-binding protein 1 (OTUB1) specifically deubiquitinates DEPTOR in a deubiquitination assay. We found that OTUB1 directly interacted with DEPTOR via its N-terminal domain, deubiquitinated DEPTOR, and thereby stabilized DEPTOR in a Cys-91–independent but Asp-88–dependent manner, suggesting that OTUB1 targets DEPTOR for deubiquitination via a deubiquitinase activity–independent non-canonical mechanism. The interaction between OTUB1 and DEPTOR was enhanced when the cells were treated with amino acids. Moreover, OTUB1 suppressed amino acid–induced activation of mTORC1 in a DEPTOR-dependent manner and thereby ultimately controlled cellular autophagy, cell proliferation, and size. Our findings reveal a mechanism that stabilizes the mTORC1 inhibitor DEPTOR via OTUB1's deubiquitinase activity. Our insights may inform research into various mTOR activity–related diseases, such as cancer, and may contribute to the identification of new diagnostic markers and therapeutic strategies for cancer treatments.
- Published
- 2018
29. A learning-based approach for leaf detection in traffic surveillance video
- Author
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Xiaoping Peng, Li Chen, Jing Tian, and Liu Jiaxiang
- Subjects
Exploit ,Computer science ,business.industry ,Applied Mathematics ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Probabilistic logic ,020206 networking & telecommunications ,02 engineering and technology ,Convolutional neural network ,Computer Science Applications ,Discriminative model ,Artificial Intelligence ,Hardware and Architecture ,Signal Processing ,0202 electrical engineering, electronic engineering, information engineering ,In vehicle ,020201 artificial intelligence & image processing ,Computer vision ,Learning based ,Artificial intelligence ,business ,Software ,Information Systems - Abstract
Traffic surveillance video is recorded in uncontrolled outdoor scenarios. If the camera view gets obstructed by the leaves, the video will fail to be used in vehicle tracking and recognition. It is required that the traffic video surveillance systems run self-checking in order to evaluate if the camera view is blocked by leaves or not. In view of this, a two-step learning framework is proposed in this paper to automatically determine whether the video is leaf degraded or leaf free. First, the proposed framework exploits the convolutional neural network to learn the discriminative features of leaf particles. Then the trained model is used to detect candidate leaf patches in the image. Second, a probabilistic approach is used to pool decisions of each candidate leaf patch to generate final leaf detection result in the video. Experimental results are provided to demonstrate that the proposed approach can effectively detect leaves in real-world traffic surveillance video.
- Published
- 2017
30. Pyrazinone derivatives from the coral-derived Aspergillus ochraceus LCJ11-102 under high iodide salt
- Author
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Tonghan Zhu, Weiming Zhu, Yi Wang, and Xiaoping Peng
- Subjects
Coral ,Biology ,Enterobacter aerogenes ,01 natural sciences ,Anti-Infective Agents ,X-Ray Diffraction ,Drug Discovery ,Animals ,Humans ,Fermentation broth ,Aspergillus ochraceus ,010405 organic chemistry ,Organic Chemistry ,Iodides ,Anthozoa ,Antimicrobial ,biology.organism_classification ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Iodide salt ,Biochemistry ,Pyrazines ,Halotolerance ,Molecular Medicine ,Antibacterial activity ,HeLa Cells ,Nuclear chemistry - Abstract
Five new pyrazin-2(1H)-one derivatives, ochramides A-D (1-4) and ochralate A (5), as well as three known analogues (6-8) were isolated from the fermentation broth of the marine coral-derived halotolerant Aspergillus ochraceus LCJ11-102 in a nutrient-limited medium containing 10% NaI. Their chemical structures were determined by analyzing NMR and X-ray diffraction data. Compounds 2, 5 and 6 showed antimicrobial activities against Enterobacter aerogenes with the MIC values of 40.0, 18.9, and 20.1 μM, respectively.
- Published
- 2017
31. Soft sediment deformation structures in the Lixian lacustrine sediments, eastern Tibetan Plateau and implications for postglacial seismic activity
- Author
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Huili Yang, Hanchao Jiang, Xiaoping Peng, Yanhao Li, Hongyan Xu, and Ning Zhong
- Subjects
geography ,Plateau ,geography.geographical_feature_category ,010504 meteorology & atmospheric sciences ,Pleistocene ,Stratigraphy ,Liquefaction ,Geology ,010502 geochemistry & geophysics ,01 natural sciences ,Soft-sediment deformation structures ,Paleontology ,Sequence (geology) ,Tectonics ,Deformation mechanism ,Clastic rock ,Geomorphology ,0105 earth and related environmental sciences - Abstract
The eastern margin of the Tibetan Plateau is characterized by frequent earthquakes, which are closely associated with tectonic activity. To assess tectonic activity during the Late Pleistocene, we studied a well-exposed, 23-m-thick lacustrine sequence on the eastern margin of the plateau and found a variety of soft-sediment deformation (SSD) structures. Distributed in 24 stratigraphic levels, they comprise clastic dykes, ball-and-pillow structures, flame structures, clastic gravels, micro-faults, and slump folds. Most of the SSD structures indicate deformation mechanisms related to liquefaction and/or fluidization processes, most probably triggered by paleoseismic events. Given at least 4 historical earthquakes of M > 7 in the study area, most SSD structures in the Lixian lacustrine sequence indicate seismic events with M > 6 while simple flame structures point to some lower-magnitude earthquakes. OSL dating indicates that the sequence accumulated between 15.8 and 6.0 ka, giving a mean recurrence interval of 480 years for the 24 events, and demonstrating that lacustrine sediments in eastern Tibet have the potential to record a continuous seismic history on the centennial scale.
- Published
- 2016
32. Letter by Zhu et al Regarding Article, 'Mild Hypothermia in Cardiogenic Shock Complicating Myocardial Infarction: Randomized SHOCK-COOL Trial'
- Author
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Zeqi Zheng, Xiaoping Peng, and Jianbing Zhu
- Subjects
medicine.medical_specialty ,Mild hypothermia ,business.industry ,medicine.medical_treatment ,Cardiogenic shock ,Percutaneous coronary intervention ,Hypothermia ,medicine.disease ,Physiology (medical) ,Internal medicine ,Shock (circulatory) ,medicine ,Cardiology ,Myocardial infarction ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business - Published
- 2019
33. Quercetin protects cardiomyocytes against doxorubicin-induced toxicity by suppressing oxidative stress and improving mitochondrial function via 14-3-3γ
- Author
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Xiaoping Peng, Xuanying Chen, Huan He, Ming He, Dong Yin, Jiegen You, Yong Luo, and Qiang Xu
- Subjects
Health, Toxicology and Mutagenesis ,Apoptosis ,010501 environmental sciences ,Mitochondrion ,Pharmacology ,Toxicology ,medicine.disease_cause ,Mitochondrial Membrane Transport Proteins ,01 natural sciences ,Antioxidants ,Mitochondria, Heart ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Animals ,Myocytes, Cardiac ,Viability assay ,Cells, Cultured ,0105 earth and related environmental sciences ,Membrane Potential, Mitochondrial ,Cardioprotection ,0303 health sciences ,Mitochondrial Permeability Transition Pore ,Chemistry ,030302 biochemistry & molecular biology ,Glutathione ,Oxidative Stress ,14-3-3 Proteins ,Doxorubicin ,Gene Knockdown Techniques ,Toxicity ,Quercetin ,Reactive Oxygen Species ,Intracellular ,Oxidative stress - Abstract
Cardiotoxicity limits the clinical applications of doxorubicin (Dox), which mechanism might be excess generation of intracellular ROS. Quercetin (Que) is a flavonoid that possesses anti-oxidative activities, exerts myocardial protection. We hypothesized that the cardioprotection against Dox injury of Que involved 14-3-3γ, and mitochondria. To investigate the hypothesis, we treated primary cardiomyocytes with Dox and determined the effects of Que pretreatment with or without 14-3-3γ knockdown. We analyzed various cellular and molecular indexes. Our data showed that Que attenuated Dox-induced toxicity in cardiomyocytes by upregulating 14-3-3γ expression. Que pretreatment increased cell viability, SOD, catalase, and GPx activities, GSH levels, MMP and the GSH/GSSG ratio; decreased LDH and caspase-3 activities, MDA and ROS levels, mPTP opening and the percentage of apoptotic cells. However, Que's cardioprotection were attenuated by knocking down 14-3-3γ expression using pAD/14-3-3γ-shRNA. In conclusion, Que protects cardiomyocytes against Dox injury by suppressing oxidative stress and improving mitochondrial function via 14-3-3γ.
- Published
- 2019
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34. EXOSOME-DERIVED CIRCITGB1 REGULATES DENDRITIC CELL MATURATION AND CARDIAC INFLAMMATION VIA MIR-342-3P/NFAM1
- Author
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Jianbing Zhu, Aiping Le, Junbo Ge, Qian Wang, Zhaoyang Chen, Zeqi Zheng, Zhenzhong Zheng, and Xiaoping Peng
- Subjects
business.industry ,Ischemia ,Inflammation ,Disease ,Dendritic cell ,medicine.disease ,Exosome ,Microvesicles ,Cancer research ,medicine ,cardiovascular diseases ,Myocardial infarction ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business - Abstract
Acute myocardial infarction (AMI) is caused by sustained ischemia and is often the manifestation of atherosclerosis-related cardiovascular disease. Circular RNAs (circRNAs) are known to be associated with cardiovascular disease and are enriched in exosomes. However, the expression profile and
- Published
- 2020
35. Discrepancy of sap flow in Salix matsudana grown under different soil textures in the water-wind erosion crisscross region on the Loess Plateau
- Author
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Xiaoping Peng, Quanjiu Wang, David N. Warrington, and Jun Fan
- Subjects
Hydrology ,Watershed ,Salix matsudana ,biology ,Soil texture ,Soil Science ,Plant Science ,Vegetation ,biology.organism_classification ,Agronomy ,Loess ,Soil water ,Environmental science ,Aeolian processes ,Transpiration - Abstract
An accurate understanding about the variation of sap flow and the interaction mechanisms of sap flow with environmental factors is essential when carrying out vegetation restoration projects in areas where rainfall is limited. A thermal dissipation probe (TDP) measured sap flow of Salix matsudana, growing in typical sandy and loess soils in the same semi-arid watershed on the Loess Plateau, China in 2012 and 2013 from May to October. Similar sap flow diurnal variation patterns occurred for both soils but, based on the sap flow, the calculated total transpiration of S. matsudana growing in the loess soil was about five times greater than that growing in the sandy soil due to differences in the sapwood cross-sectional area. Soil texture affected both the vertical distribution of S. matsudana fine roots and the soil water cycle, which led to the S. matsudana growing in the sandy soil being subjected more frequently to drought stress that stunted its growth. In contrast, S. matsudana grew well in the loess soil. Soil texture was the key factor responsible for the discrepancy in the total sap flow of S. matsudana in the study region due to its effect on soil water content. Therefore, if afforestation is planned for this or similar regions, soil texture should be taken into account.
- Published
- 2014
36. Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
- Author
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Yu Liu, Wei Dong, Angie G. Aceves, Xiaoping Peng, Rui Liang, and Xiaopeng Shen
- Subjects
0301 basic medicine ,musculoskeletal diseases ,Cellular differentiation ,General Chemical Engineering ,Biology ,Transfection ,Myotonic dystrophy ,General Biochemistry, Genetics and Molecular Biology ,Cell Line ,Myoblasts ,03 medical and health sciences ,Transduction (genetics) ,Mice ,medicine ,Myocyte ,Animals ,Myotonic Dystrophy ,Muscular dystrophy ,General Immunology and Microbiology ,Myogenesis ,General Neuroscience ,Cell Differentiation ,medicine.disease ,musculoskeletal system ,Molecular biology ,Cell biology ,030104 developmental biology ,Medicine ,C2C12 ,tissues - Abstract
Myotonic dystrophy 1 (DM1) is a common form of muscular dystrophy. Although several animal models have been established for DM1, myoblast cell models are still important because they offer an efficient cellular alternative for studying cellular and molecular events. Though C2C12 myoblast cells have been widely used to study myogenesis, resistance to gene transfection, or viral transduction, hinders research in C2C12 cells. Here, we describe an optimized protocol that includes daily maintenance, transfection and transduction procedures to introduce genes into C2C12 myoblasts and the induction of myocyte differentiation. Collectively, these procedures enable best transfection/transduction efficiencies, as well as consistent differentiation outcomes. The protocol described in establishing DM1 myoblast cell models would benefit the study of myotonic dystrophy, as well as other muscular diseases.
- Published
- 2016
37. SET1A-Mediated Mono-Methylation at K342 Regulates YAP Activation by Blocking Its Nuclear Export and Promotes Tumorigenesis
- Author
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Guanghong Liao, Yongzhi Yang, Chenchen Jiao, Ping Wang, Jun Zhou, Xiaoping Peng, Lujian Liao, Linjun Weng, Qing Wei, Jiali Jin, Shihao Zhang, Yaxu Li, Xinbo Wang, Hongqi Teng, Xueling Jin, Bin Zhao, Yilin Wang, Zhongchen Liu, Dawang Zhou, Jiayu Chen, Lei Chen, Houqin Fang, Cheng Li, Min Liu, Lan Fang, Huanlong Qin, Xin Ge, and Dongyan Han
- Subjects
0301 basic medicine ,Cancer Research ,Lung Neoplasms ,Active Transport, Cell Nucleus ,Cell Cycle Proteins ,Chromosomal translocation ,medicine.disease_cause ,Methylation ,03 medical and health sciences ,Gene expression ,medicine ,Animals ,Humans ,Protein Interaction Domains and Motifs ,Nuclear export signal ,Adaptor Proteins, Signal Transducing ,Cell Proliferation ,Cell Nucleus ,Mice, Knockout ,Hippo signaling pathway ,Effector ,Chemistry ,Lysine ,YAP-Signaling Proteins ,Histone-Lysine N-Methyltransferase ,Phosphoproteins ,Prognosis ,Tumor Burden ,Cell biology ,Mice, Inbred C57BL ,Cell Transformation, Neoplastic ,HEK293 Cells ,030104 developmental biology ,Oncology ,A549 Cells ,Cytoplasm ,Colorectal Neoplasms ,Carcinogenesis ,Protein Processing, Post-Translational ,HeLa Cells ,Protein Binding ,Signal Transduction ,Transcription Factors - Abstract
Summary YAP, a key effector of Hippo pathway, is activated by its translocation from cytoplasm to nucleus to regulate gene expression and promote tumorigenesis. Although the mechanism by which YAP is suppressed in cytoplasm has been well-studied, how the activated YAP is sequestered in the nucleus remains unknown. Here, we demonstrate that YAP is a nucleocytoplasmic shuttling protein and its nuclear export is controlled by SET1A-mediated mono-methylation of YAP at K342, which disrupts the binding of YAP to CRM1. YAP mimetic methylation knockin mice are more susceptible to colorectal tumorigenesis. Clinically, YAP K342 methylation is reversely correlated with cancer survival. Collectively, our study identifies SET1A-mediated mono-methylation at K342 as an essential regulatory mechanism for regulating YAP activity and tumorigenesis.
- Published
- 2018
38. Prognostic factors for patients with FIGO Stage-IB cervical squamous cell carcinoma: Does the tumor size (≤ 4 cm or > 4 cm) really matter?
- Author
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Yang Shen, Ying Xiong, Mei Wei, Li-Zhi Liang, and Xiaoping Peng
- Subjects
Oncology ,medicine.medical_specialty ,endocrine system diseases ,Tumor size ,Cervical Squamous Cell Carcinoma ,business.industry ,fungi ,food and beverages ,female genital diseases and pregnancy complications ,Stage ib ,Internal medicine ,medicine ,Radical surgery ,business ,Cervical neoplasm ,Earth-Surface Processes - Abstract
Objective To investigate the factors that can accurately predict the prognosis for patients with FIGO stage-IB cervical squamous cell carcinoma treated with radical surgery.
- Published
- 2007
39. Research on New Method of Clastic Reservoir Permeability Interpretation
- Author
-
Xing Zhang, Shengqun Dai, Hong Fang, Wei Liu, and Xiaoping Peng
- Subjects
Permeability (earth sciences) ,Approximation error ,Capillary action ,Well logging ,Geotechnical engineering ,Mechanics ,Empirical relationship ,Porosity ,Hagen–Poiseuille equation ,Tortuosity ,Geology - Abstract
Reservoir permeability is an important parameter in reservoir evaluation and the research on surplus oil distributing regularities. However, it is difficult to calculate it accurately in the process of reservoir interpretation. The ordinary interpretation model uses the rough linear relationship between porosity and permeability within a semi-log coordinate system, resulting in much error. In this paper the permeability calculating formula, including three parameters, porosity, pore-throat radius and pore tortuosity, was deduced from the unity of Poiseuille Capillary Model and Darcy’s Law. Based on that, data of core physical properties analysis, mercury injection and well logging were used to construct the empirical relationship between pore tortuosity and pore-throat radius, thus realizing the transformation of permeability calculation from the solo empirical model to the semi-theoretical and semi-empirical model. The calculated results showed that the relative error of the new model was 20.26%, with 22.46 percentage points lower than the error of the traditional empirical model.
- Published
- 2012
40. Cerebrosides and 2-pyridone alkaloids from the halotolerant fungus Penicillium chrysogenum grown in a hypersaline medium
- Author
-
Weiming Zhu, Peipei Liu, Yi Wang, Xia Yin, Xiaoping Peng, and Kunlai Sun
- Subjects
Salinity ,Stereochemistry ,Pharmaceutical Science ,Fungus ,Microbial Sensitivity Tests ,Biology ,Penicillium chrysogenum ,Enterobacter aerogenes ,Analytical Chemistry ,Alkaloids ,Cerebrosides ,Drug Discovery ,Pharmacology ,Molecular Structure ,Organic Chemistry ,Fungi imperfecti ,biology.organism_classification ,Antimicrobial ,Cerebroside ,Anti-Bacterial Agents ,Complementary and alternative medicine ,Biochemistry ,Halotolerance ,Molecular Medicine ,Bacteria - Abstract
Five new cerebrosides, chrysogesides A-E (1-5), and two new 2-pyridone alkaloids, chrysogedones A and B (6 and 7), were isolated from the fermentation broth of Penicillium chrysogenum PXP-55, a halotolerant fungus grown in a hypersaline medium. Among them, chrysogesides B-D (2-4) are the first cerebrosides that contain an unsaturated C(19)-fatty acid. Their structures were identified by spectroscopic and chemical methods, including CD spectroscopy as well as the modified Mosher's method. Compound 2 showed antimicrobial activity against Enterobacter aerogenes with an MIC value of 1.72 μM.
- Published
- 2011
41. Novel cyclic hexapeptides from marine-derived fungus, Aspergillus sclerotiorum PT06-1
- Author
-
Jinkai Zheng, Kui Hong, Yi Wang, Xin Wang, Huajie Zhu, Xiaoping Peng, Peipei Liu, and Weiming Zhu
- Subjects
Models, Molecular ,Staphylococcus aureus ,Antifungal Agents ,Photoisomerization ,Stereochemistry ,Radical ,Molecular Conformation ,Stereoisomerism ,Antineoplastic Agents ,HL-60 Cells ,Microbial Sensitivity Tests ,Crystallography, X-Ray ,Biochemistry ,Peptides, Cyclic ,chemistry.chemical_compound ,Structure-Activity Relationship ,Candida albicans ,Anthranilic acid ,Escherichia coli ,Structure–activity relationship ,Humans ,Physical and Theoretical Chemistry ,Cytotoxicity ,Cell Proliferation ,Organic Chemistry ,Enterobacter aerogenes ,Anti-Bacterial Agents ,Aspergillus ,chemistry ,Pseudomonas aeruginosa ,Halotolerance ,Drug Screening Assays, Antitumor ,Antibacterial activity ,Bacillus subtilis - Abstract
Two novel cyclic hexapeptides containing both anthranilic acid and dehydroamino acid units, sclerotides A (1) and B (2), were isolated from the marine-derived halotolerant Aspergillus sclerotiorum PT06-1 in a nutrient-limited hypersaline medium. Both 1 and 2 are photointerconvertible and could be interconverted via a radical reaction initiated by direct photoisomerization. Both compounds showed moderate antifungal activity. Compound 2 also showed weak cytotoxicity and antibacterial activity.
- Published
- 2009
42. Cerebrosides of the halotolerant fungus Alternaria raphani isolated from a sea salt field
- Author
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Hongwen Tao, Xiaoping Peng, Yi Wang, Wen-Liang Wang, Weiming Zhu, and Peipei Liu
- Subjects
Pharmaceutical Science ,Marine Biology ,Bacillus subtilis ,Microbial Sensitivity Tests ,Crystallography, X-Ray ,Analytical Chemistry ,Alkaloids ,Cerebrosides ,Drug Discovery ,Botany ,Candida albicans ,Escherichia coli ,Seawater ,Food science ,Antibacterial agent ,Pharmacology ,biology ,Alternaria raphani ,Molecular Structure ,Organic Chemistry ,Alternaria ,Fungi imperfecti ,biology.organism_classification ,Anti-Bacterial Agents ,Complementary and alternative medicine ,Halotolerance ,Molecular Medicine ,Salts ,Antibacterial activity - Abstract
In order to search for structurally novel and bioactive natural compounds from marine-derived fungi, a halotolerant fungal strain (THW-18) identified as Alternaria raphani was isolated from sediment collected in the Hongdao sea salt field. From the ethyl acetate extract of Alternaria raphani, three new cerebrosides, alternarosides A-C (1-3), and a new diketopiperazine alkaloid, alternarosin A (4), together with 15 known compounds were isolated and identified by spectroscopic and chemical methods, as well as X-ray crystal diffraction analysis. Compounds 1-4 showed weak antibacterial activity against Escherichia coli, Bacillus subtilis, and Candida albicans with MIC values ranging from 70 to 400 muM.
- Published
- 2009
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