18 results on '"YOSHIDA"'
Search Results
2. Regulation of mechanical stress-induced MMP-13 and ADAMTS-5 expression by RUNX-2 transcriptional factor in SW1353 chondrocyte-like cells
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Taichi Saito, Toshifumi Ozaki, Keiji Naruse, Tomoko Tetsunaga, Aki Yoshida, Keiichiro Nishida, Takayuki Furumatsu, and Satoshi Hirohata
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MAPK/ERK pathway ,Small interfering RNA ,Mechanical stress ,MAP Kinase Signaling System ,Biomedical Engineering ,ADAMTS ,Down-Regulation ,Core Binding Factor Alpha 1 Subunit ,Biology ,Polymerase Chain Reaction ,p38 Mitogen-Activated Protein Kinases ,Chondrocytes ,Rheumatology ,Matrix Metalloproteinase 13 ,Gene expression ,Humans ,Orthopedics and Sports Medicine ,Enzyme Inhibitors ,Protein kinase A ,Transcription factor ,Cells, Cultured ,Regulation of gene expression ,Messenger RNA ,Chondrocyte ,RUNX-2 ,Molecular biology ,Up-Regulation ,ADAM Proteins ,Gene Expression Regulation ,ADAMTS5 Protein ,Stress, Mechanical ,Aggrecanase - Abstract
Summary Objective To investigate the mechanism of mechanical stress-induced expression and regulation of aggrecanases and examine the role of runt-related transcription factor 2 (RUNX-2) in chondrocyte-like cells. Methods SW1353 cells were seeded onto stretch chambers at a concentration of 5×10 4 cells/chamber, and a uni-axial cyclic tensile strain (CTS) (0.5Hz, 10% stretch) was applied for 30min. Total RNA was extracted, reverse transcribed, and analyzed by polymerase chain reaction (PCR) and real-time PCR. RUNX-2 overexpression and small interfering RNA (siRNA) targeting RUNX-2 were used to investigate the role of RUNX-2 in CTS-induced gene expression. The involvement of diverse mitogen-activated protein kinase (MAPK) pathways in the activation of RUNX-2, MMP-13 and ADAMTS-5 during CTS was examined by Western blotting. Results CTS induced expression of RUNX-2 , MMP-13 , ADAMTS-4 , -5 , and -9 . Overexpression of RUNX-2 up-regulated expression of MMP-13 and ADAMTS-5 , whereas RUNX-2 siRNA resulted in significant down-regulation of mechanically-induced MMP-13 and ADAMTS-5 expression. CTS induced activation of p38 MAPK, and CTS induction of RUNX-2 , MMP-13 and ADAMTS-5 mRNA was down-regulated by the selective p38 MAPK inhibitor SB203580 but not by the p44/42 MAPK inhibitor U0126, or the JNK MAPK inhibitor JNK inhibitor II. Conclusions RUNX-2 might have a role as a key downstream mediator of p38's ability to regulate mechanical stress-induced MMP-13 and ADAMTS-5 expression.
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- 2011
3. Ubiquitous expression of galectin-3 mRNA in benign and malignant thyroid tumors
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Fumio Matsuzuka, Akira Miyauchi, Nobuyuki Amino, Hiroshi Yoshida, Kanji Kuma, and Toru Takano
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Adenoma ,endocrine system ,Cancer Research ,Pathology ,medicine.medical_specialty ,Transcription, Genetic ,endocrine system diseases ,Galectin 3 ,Molecular Sequence Data ,Biology ,Polymerase Chain Reaction ,Thyroid carcinoma ,Adenocarcinoma, Follicular ,Gene expression ,Biopsy ,otorhinolaryngologic diseases ,medicine ,Carcinoma ,Humans ,RNA, Messenger ,Thyroid Neoplasms ,DNA Primers ,Messenger RNA ,Base Sequence ,medicine.diagnostic_test ,Thyroid ,medicine.disease ,Gene Expression Regulation, Neoplastic ,stomatognathic diseases ,medicine.anatomical_structure ,Oncology ,Immunohistochemistry ,Endocrine gland - Abstract
We measured the relative expression levels of galectin-3 mRNA to beta-actin mRNA in normal thyroid tissues, thyroid tumor tissues and thyroid-derived fibroblasts. Galectin-3 mRNA was expressed ubiquitously in both benign and malignant thyroid tumors. Although a significant increase in its expression was observed in papillary carcinomas, no significant difference was observed between follicular carcinomas and adenomas. In contrast to the previous optimistic reports using immunohistochemical analysis of the galectin-3 protein expression, these results demonstrate that galectin-3 mRNA may not be a suitable target for molecular-based diagnosis of thyroid carcinomas.
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- 2003
4. Five novelSLC7A7 variants and y+L gene-expression pattern in cultured lymphoblasts from Japanese patients with lysinuric protein intolerance
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Yasuko Shoji, Shuichi Yamaguchi, Kenji Ihara, Masaki Takayanagi, Yutaka Shoji, Akio Koizumi, Atsuko Noguchi, Youichi Hokezu, Makoto Yoshino, Akio Nakai, Masayuki Kaji, Keiji Nagamatsu, Mika Matsumori, Isao Kitajima, Yoshihiro Yoshida, Yuhei Takasago, Goro Takada, Shigenori Yamamoto, Toshiro Hara, and Hitoshi Mikami
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Male ,Adolescent ,Transcription, Genetic ,DNA Mutational Analysis ,Molecular Sequence Data ,Biology ,Lymphocyte Activation ,law.invention ,Japan ,law ,Gene expression ,Genetics ,medicine ,Humans ,Lymphocytes ,RNA, Messenger ,Amino acid transporter ,Child ,Gene ,Cells, Cultured ,Genetics (clinical) ,Polymerase chain reaction ,Cationic Amino Acid Transporter 1 ,Messenger RNA ,Base Sequence ,Fusion Regulatory Protein 1, Light Chains ,Lymphoblast ,Amino Acid Transport System y+L ,Genetic Variation ,medicine.disease ,Molecular biology ,Lysinuric protein intolerance ,Small intestine ,medicine.anatomical_structure ,Biochemistry ,Mutation ,Amino Acid Transport Systems, Basic ,Female ,Amino Acid Transport Disorders, Inborn - Abstract
Two distinct human light subunits of the heteromeric amino acid transporter, y+LAT-1 coded by SLC7A7 and y+LAT-2 coded by SLC7A6, are both known to induce transport system y+L activity. SLC7A7 has already been identified as the gene responsible for lysinuric protein intolerance (LPI). We successfully identified five novel SLC7A7 variants (S238F, S489P, 1630delC, 1673delG, and IVS3-IVS5del9.7kb) in Japanese patients with LPI by PCR amplification and direct DNA sequencing. In addition, we performed a semi-quantitative expression analysis of SLC7A7 and SLC7A6 in human tissue. In normal tissue, the gene-expression ratio of SLC7A6 to SLC7A7 was high in the brain, muscle, and cultured skin fibroblasts; low in the kidneys and small intestine; and at an intermediate level in peripheral blood leukocytes, the lungs, and cultured lymphoblasts. The gene-expression ratio of SLC7A6 to SLC7A7 in cultured lymphoblasts was significantly different between normal subjects and LPI patients with R410X and/or S238F, where the relative amount of SLC7A7 mRNA was significantly lower and the relative amount of SLC7A6 mRNA was statistically higher in affected lymphoblasts than in normal cells. Expression of SLC7A7 and SLC7A6 may thus be interrelated in cultured lymphoblasts. Hum Mutat 20:375–381, 2002. © 2002 Wiley-Liss, Inc.
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- 2002
5. Regulation of obese mRNA expression by hormonal factors in primary cultures of rat adipocytes
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Toshiaki Monkawa, Takao Saruta, Tadashi Yoshida, and Matsuhiko Hayashi
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Male ,medicine.medical_specialty ,DNA, Complementary ,Time Factors ,Transcription, Genetic ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Biology ,Polymerase Chain Reaction ,Dexamethasone ,Propanolamines ,chemistry.chemical_compound ,Endocrinology ,Internal medicine ,Adipocyte ,Gene expression ,Adipocytes ,medicine ,Animals ,Insulin ,Obesity ,RNA, Messenger ,Cloning, Molecular ,Rats, Wistar ,Cells, Cultured ,Messenger RNA ,Leptin ,General Medicine ,Adrenergic beta-Agonists ,Rats ,chemistry ,Homeostasis ,Hormone ,medicine.drug - Abstract
Yoshida T, Hayashi M, Monkawa T, Saruta T. Regulation of obese mRNA expression by hormonal factors in primary cultures of rat adipocytes. Eur J Endocrinol 1996;135:619–25. ISSN 0804–4643 The obese (ob) gene has been cloned recently and its protein product is called "leptin". Leptin is an adipocyte-derived satiety factor that regulates body weight homeostasis. Several hormonal factors have been reported to regulate ob mRNA expression. To determine which factors are most important for regulation of ob mRNA expression, we examined the effects of insulin, dexamethasone, a β3-adrenergic agonist (CGP12177A), 8-bromo-cAMP, 8-bromo-cGMP and 1-methyl-3-isobutylxanthine (MIX) on primary cultured adipocytes. Rat adipocytes obtained from epididymal fat were cultured using the ceiling method. Total RNA was extracted and the expression of ob mRNA was measured by quantitative reverse transcription-polymerase chain reaction. After 24 h of incubation, 100 nmol/l insulin significantly increased the expression of ob mRNA (21.4-fold compared to control). Moreover, insulin increased ob mRNA expression in a dose-dependent manner over a range of 1–100 nmol/l. The effect of 100 nmol/l insulin was similar to that seen with 20% newborn calf serum. Dexamethasone (25–1000 nmol/l) also increased ob mRNA expression (2.5–2.9-fold). The effect of dexamethasone occurred more rapidly than insulin. CGP12177A (1–10 μmol/l) and 0.5 mmol/l 8-bromo-cAMP had no effects, whereas 0.5 mmol/l 8-bromo-cGMP and 0.5 mmol/l MIX had stimulatory effects (2.8- and 2.4-fold increase in ob mRNA, respectively). The combination of 250 nmol/l dexamethasone and 0.5 mmol/l MIX did not have an additive effect on ob mRNA levels. Our present data suggest that, of these agents, insulin is the most important factor regulating ob mRNA expression. Matsuhiko Hayashi, Department of Internal Medicine, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan
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- 1996
6. Pancreatic Islet Cells Express BST-1, a CD38-like Surface Molecule Having ADP-Ribosyl Cyclase Activity
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Hirotaka Watada, Toshio Hirano, Katsuhiko Ishihara, Takenobu Kamada, Toshiaki Hanafusa, Tsuneyasu Kaisho, Motoyuki Itoh, Yoshimitsu Yamasaki, Hideyuki Yoshida, Jun-ichiro Miyagawa, Yoshiki Okuyama, Yoshio Fujitani, Yoshitaka Kajimoto, Taka-aki Matsuoka, and Yuji Matsuzawa
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Glycosylphosphatidylinositols ,Biophysics ,Gene Expression ,CD38 ,Biology ,GPI-Linked Proteins ,Transfection ,Polymerase Chain Reaction ,Biochemistry ,Cyclase ,Islets of Langerhans ,Mice ,Antigens, CD ,Aplysia ,medicine ,Animals ,Humans ,Point Mutation ,RNA, Messenger ,ADP-ribosyl Cyclase ,N-Glycosyl Hydrolases ,Molecular Biology ,chemistry.chemical_classification ,Mice, Inbred BALB C ,Messenger RNA ,Membrane Glycoproteins ,Sequence Homology, Amino Acid ,Pancreatic islets ,Cell Biology ,Flow Cytometry ,ADP-ribosyl cyclase activity ,ADP-ribosyl Cyclase 1 ,Antigens, Differentiation ,Immunohistochemistry ,Recombinant Proteins ,Cell biology ,medicine.anatomical_structure ,Enzyme ,chemistry ,Antigens, Surface ,Intracellular - Abstract
Cyclic ADP-ribose (cADPR), a well-known stimulator of ca(2+) release from the intracellular Ca(2+) pool, has recently emerged as a potential regulator of insulin secretion in pancreatic beta cells. As recently described, BST-1 is a glycosyl-phosphatidylinositol (GPI)-anchored surface molecule that exhibits homology with CD38 and Aplysia ADP-ribosyl cyclase. Like CD38, BST-1 has both ADP-ribosyl cyclase and cADPR hydrolase activities. As a step toward elucidating the physiological role of cADPR in insulin secretion we examined whether BST-1 is expressed in pancreatic islet cells. Sensitive reverse transcription-polymerase chain reaction detected almost as abundant expression of BST-1 mRNA in pancreatic islets as CD38 mRNA. Immunohistochemical analyses utilizing mirror image sections revealed that BST-1 protein is expressed in a majority of the cells in pancreatic islets and that at least beta cells and, to an even greater extent, alpha cells express BST-1. These observations suggest the involvement of multiple enzymes in the regulation of cADPR concentrations in pancreatic islet cells.
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- 1996
7. Growth inhibition of subcutaneously transplanted human glioma by transfection-induced tumor necrosis factor-? and augmentation of the effect by ?-interferon
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Kenichiro Sugita, Tohru Uozumi, Masaaki Mizuno, Jun Yoshida, Kaoru Kurisu, and Kunyu Harada
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Cancer Research ,Time Factors ,medicine.medical_treatment ,Molecular Sequence Data ,Intraperitoneal injection ,Gene Expression ,Mice, Nude ,Biology ,Transfection ,Polymerase Chain Reaction ,Cell Line ,Interferon-gamma ,Mice ,chemistry.chemical_compound ,Glioma ,Tumor Cells, Cultured ,medicine ,Animals ,Humans ,RNA, Messenger ,DNA Primers ,Messenger RNA ,Liposome ,Base Sequence ,medicine.diagnostic_test ,Tumor Necrosis Factor-alpha ,Genetic Therapy ,medicine.disease ,Combined Modality Therapy ,Survival Analysis ,Molecular biology ,Neurology ,Oncology ,chemistry ,Immunoassay ,Liposomes ,Female ,Tumor necrosis factor alpha ,Neurology (clinical) ,Growth inhibition ,Cell Division - Abstract
Using subcutaneous solid tumors produced by U251-MG human glioma cells, we studied the in vivo transfection of the cells with the tumor necrosis factor-alpha (TNF-alpha) gene delivered by means of liposomes. When the tumor had become 7 mm in diameter, liposomes with entrapped TNF-alpha gene were injected into the center of the subcutaneous tumor. We found that mRNA of transfection-induced TNF-alpha, which was expressed in the tumor tissue, was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) method and its protein was demonstrated by enzyme-linked immunoassay. Growth of the tumor was inhibited when the injection was carried out five times at every other day. The growth-inhibitory effect by transfection-induced TNF-alpha was much remarkable as compared with exogenous TNF-alpha and the effect was enhanced by the intraperitoneal injection of gamma-interferon (IFN-gamma) 12 h prior to intratumoral injection of the liposomes.
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- 1994
8. Erdheim-Chester disease: report of a case with PCR-based analysis of the expression of osteopontin and survivin in Xanthogranulomas following glucocorticoid treatment
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Hideaki Nakajima, Fuminori Ikeno, Kozo Hashimoto, Yasumasa Iwasaki, Toshihiro Takao, Koichi Asaba, Hajime Kodama, Takafumi Taguchi, and Toshinori Yoshida
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Adult ,medicine.medical_specialty ,Pathology ,Erdheim-Chester Disease ,genetic structures ,Endocrinology, Diabetes and Metabolism ,Prednisolone ,Survivin ,Gene Expression ,Disease ,Polymerase Chain Reaction ,Inhibitor of Apoptosis Proteins ,Endocrinology ,stomatognathic system ,Internal medicine ,medicine ,Xanthomatosis ,Humans ,Osteopontin ,Glucocorticoids ,Messenger RNA ,Granuloma ,biology ,business.industry ,medicine.disease ,eye diseases ,Neoplasm Proteins ,Histiocytosis ,Diabetes insipidus ,Erdheim–Chester disease ,biology.protein ,Female ,business ,Microtubule-Associated Proteins ,Glucocorticoid ,medicine.drug ,Follow-Up Studies - Abstract
Erdheim-Chester disease (ECD) is a form of non-Langerhans histiocytosis. In this report, we show a case of ECD presenting diabetes insipidus and multiple xanthogranulomas received glucocorticoid treatment over a year. During this period, xanthogranulomas improved in response to the glucocorticoid therapy. Furthermore, the expression of osteopontin in xanthogranulomatous tissues significantly decreased following the treatment. Our data show the expression of osteopontin in xanthogranulomatous tissues of ECD. Furthermore, the osteopontin mRNA decreased following glucocorticoid therapy with xanthogranuloma regression, suggesting that the expression level of osteopontin could be a marker of the disease activity of ECD.
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- 2008
9. A faithful method for PCR-mediated global mRNA amplification and its integration into microarray analysis on laser-captured cells
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Hiromi Sakamoto, Kazuhiko Aoyagi, Shingo Akimoto, Hiroki Sasaki, Shin Ichi Hayashi, Masaaki Terada, Chikako Tanabe, Yoko Omoto, Michiie Sakamoto, Takeshi Tatsuta, Michiko Nishigaki, and Teruhiko Yoshida
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Molecular Sequence Data ,Biophysics ,Breast Neoplasms ,Biology ,Biochemistry ,Polymerase Chain Reaction ,RNA, Complementary ,Transcription (biology) ,Stomach Neoplasms ,Gene expression ,Tumor Cells, Cultured ,Humans ,RNA, Messenger ,Molecular Biology ,Gene ,Microdissection ,Oligonucleotide Array Sequence Analysis ,Messenger RNA ,Microarray analysis techniques ,Gene Expression Profiling ,Lasers ,Reproducibility of Results ,Cell Biology ,Molecular biology ,Gene expression profiling ,Gene Expression Regulation ,Cancer cell ,Female - Abstract
Quantitative and qualitative analyses of mRNAs from a small number of cells are extremely important for studies on gene expression in various physiological and pathological conditions in multicellular organisms. We present here an effective method for high-fidelity global mRNA amplification for in vivo gene expression profiling of as few as 100 cells obtained by laser-captured microdissection (LCM). This method, called TALPAT, is based on T7 RNA polymerase-mediated transcription, adaptor ligation, and PCR amplification followed by T7-transcription. More than 80% of genes were commonly identified as a more than 3-fold changed gene among three gastric cancer cell lines using cRNA amplified by both TALPAT and the ordinary in vitro T7-transcription. The reproducibility of TALPAT was validated by microarray analysis on 100 breast cancer cells obtained by LCM. For the application of the LCM-TALPAT method, we successfully obtained expression profiles of gastric cancer cells and the mesenchymal cells, enabling us to understand in vivo cell-to-cell cross-talk in the microenvironment.
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- 2003
10. Sequential alteration of proto-oncogene expression in liver, spleen, kidney and brain of mice subjected to whole body irradiation
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Norihiko Hamada, Masako Terashima, Toshiji Saibara, Akihito Nishioka, Harumichi Seguchi, Yasuhiro Ogawa, Masafumi Ono, Taisuke Inomata, Shoji Yoshida, and Saburo Onishi
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Cancer Research ,medicine.medical_specialty ,Transcription, Genetic ,Ratón ,Molecular Sequence Data ,Gene Expression ,Spleen ,Biology ,Kidney ,Polymerase Chain Reaction ,Andrology ,Mice ,Internal medicine ,Proto-Oncogenes ,medicine ,Animals ,Radiosensitivity ,RNA, Messenger ,Messenger RNA ,Mice, Inbred C3H ,Oncogene ,Base Sequence ,Brain ,Genes, fos ,General Medicine ,Reverse transcription polymerase chain reaction ,Endocrinology ,medicine.anatomical_structure ,Oncology ,Liver ,Organ Specificity ,Toxicity ,Whole-Body Irradiation - Abstract
Reverse transcription polymerase chain reaction (RT-PCR) was performed to evaluate the sequential alteration of proto-oncogene mRNA expression in liver, spleen, kidney and brain of mice after whole body irradiation (WBI). The mRNAs investigated in this study were Fas, c-fos, c-myc. bcl-2, and p53, and glyceraldehyde-3-phosphate dehydrogenase mRNA was employed as internal control. C3H/He mice aged 9-10 weeks were exposed to WBI of 7 Gy using a cobalt-60 teletherapy unit, without anesthesia, and sacrificed before and 0.1, 0.5, 1, 2, 3, 6, 12, 24, 48 and 96 h after irradiation. Their liver, spleen, kidney and brain were taken and immediately stored in liquid nitrogen until ready for RT-PCR. Each specimen was homogenized to extract RNA for conventional RT-PCR. The liver of mice administered 7 Gy of WBI revealed no significant changes in the expression of each of the mRNAs examined. In the spleen, c-fos mRNA expression decreased at 2 h following irradiation, and increased remarkably thereafter. In the kidney, no significant change in the expression of each mRNA was shown. In the brain c-fos mRNA expression decreased 1-24 h after irradiation, and showed a recovery thereafter. The remarkable differences in the sequential changes of c-fos mRNA expression following irradiation between each organ revealed by the present experiment may be an important aid in determining the tissue-specific radiosensitivity to ionizing radiation. Further investigations are, however, needed to clarify the signal transduction mechanisms which are mediated by the expression of these proto-oncogenes in each tissue following irradiation.
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- 1996
11. Expression of multidrug resistance-related genes (mdrl, MRP, GST-pi and DNA topoisomerase II) in urothelial cancers
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Wun-Jae Kim, M. Fukumoto, Osamu Yoshida, Y. Kakehi, and W.-J. Wu
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Messenger RNA ,Urologic Neoplasms ,business.industry ,Urology ,Gene Expression ,Drug resistance ,medicine.disease_cause ,Phenotype ,Drug Resistance, Multiple ,law.invention ,Multiple drug resistance ,DNA Topoisomerases, Type II ,law ,Immunology ,Gene expression ,Cancer research ,Medicine ,Humans ,business ,Carcinogenesis ,Gene ,Polymerase chain reaction ,Glutathione Transferase - Abstract
Objective To characterize the multidrug resistance (MDR) phenotype in human urothelial cancers, the expression levels of four MDR-related genes (multidrug resistance, mdrl; multidrug resistance-associated protein, MRP; glutathione S-transferase-π, GST-π; and DNA topoisomerase II, topo II) were analysed in urothelial cancers. Materials and methods Fifty-two tumour tissue and three normal urothelial mucosa samples were obtained from 44 patients with urothelial cancers. The expression of each gene was analysed with a reverse-transcription polymerase chain reaction (RT-PCR) method using β2-microglobulin (b2m) mRNA as an endogenous control. Levels of expression were expressed as the ratio of the specific products of the target gene to those specific to b2m. Results In primary urothelial cancer tissues, the mean (sd) expression of mdrl, MRP, GST-πand topo II relative to b2m expression were 0.067 (0.061), 0.27 (0.23), 0.35 (0.31) and 0.12 (0.05), respectively. The mean expressions of MRP and GST-πwere higher than those of mdrl and topo II. The mean ratios of mdrl/b2m, MRP/b2m, GST-π/b2m and topo II/b2m in normal urothelial mucosa were 0.06 (0.03), 0.12 (0.09), 0.30 (0.32) and 0.14 (0.01), respectively. There was no significant association of the expression of each gene with either the grade or extent of the primary tumour. The level of MRP expression in each sample was correlated significantly with the expression of mdrl and GST-πin the urothelial cancers (r=0.637 and 0.537, respectively). Chemotherapy did not markedly influence the induction of expression of the MDR-related genes, except for one case in which mdrl expression was 15 times greater than before chemotherapy. The expression of GST-πin the patients not receiving chemotherapy was significantly higher than in those that did. Conclusions These results suggest that the activation of MRP and GST-πexpression occurs during the tumorigenesis of urothelial cancers and that it may confer de novo and acquired drug resistance on urothelial cancers. These results should provide further insight into the complex role postulated for MDR-related genes in chemotherapy, carcinogenesis and tumour progression.
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- 1996
12. Expression and activity-dependent changes of a novel limbic-serine protease gene in the hippocampus
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Keiko Kato, Yoshiharu Momota, ZL Chen, Jun Suzuki, S Aimoto, T Tanaka, H Nishino, Shigetaka Yoshida, J Ito, and Hiroshi Kiyama
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DNA, Complementary ,Transcription, Genetic ,Molecular Sequence Data ,Hippocampus ,Gene Expression ,In situ hybridization ,Hippocampal formation ,Biology ,Polymerase Chain Reaction ,Mice ,Limbic system ,Complementary DNA ,medicine ,Kindling, Neurologic ,Limbic System ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Messenger RNA ,Base Sequence ,Kindling ,General Neuroscience ,Serine Endopeptidases ,Articles ,Blotting, Northern ,Electric Stimulation ,Cell biology ,medicine.anatomical_structure ,nervous system ,Kallikreins ,Neuroscience ,KALLIKREIN 8 - Abstract
A novel murine cDNA which encodes a protein designated neuropsin was cloned. Northern and in situ hybridization analyses demonstrated that neuropsin mRNA is expressed specifically in the limbic system of mouse brain and is localized at highest concentration in pyramidal neurons of the hippocampal CA1–3 subfields. Direct hippocampal stimulation and kindling induced by amygdaloid stimulation caused a significant bilateral change in neuropsin mRNA level in the hippocampal pyramidal neurons. The activity-dependent changes and the specific localization indicate that neuropsin is involved in hippocampal plasticity.
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- 1995
13. Cloning of the rat Gadd45 cDNA and its mRNA expression in the brain
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Edward L. Schneider, Toru Yoshida, and Nozomu Mori
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DNA, Complementary ,DNA damage ,Molecular Sequence Data ,Hamster ,Gene Expression ,Biology ,Polymerase Chain Reaction ,law.invention ,law ,Complementary DNA ,Cricetinae ,Sequence Homology, Nucleic Acid ,Genetics ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Gene ,DNA Primers ,Cloning ,Messenger RNA ,Base Sequence ,cDNA library ,Intracellular Signaling Peptides and Proteins ,Brain ,Proteins ,General Medicine ,Molecular biology ,Rats ,Gamma Rays ,Protein Biosynthesis ,Recombinant DNA ,DNA Damage - Abstract
We cloned the rat Gadd45 (growth arrest and DNA damage inducible) cDNA and examined its mRNA induction by y-ray irradiation in the rat brain. The rat Gadd45 cDNA sequence was highly homologous to the previously published human and hamster cDNAs, and was partially similar to the 28S rRNA gene. The mRNA encoding rat GADD45 was induced in the brain after γ-ray irradiation. This finding indicates that Gadd45 is an inducible gene following the ionizing radiation, not only in cultured cells in vitro, but also in animal tissues in vivo.
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- 1994
14. Novel expression of claudin-5 in glomerular podocytes.
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Koda, Ryo, Zhao, Linning, Yaoita, Eishin, Yoshida, Yutaka, Tsukita, Sachiko, Tamura, Atsushi, Nameta, Masaaki, Zhang, Ying, Fujinaka, Hidehiko, Magdeldin, Sameh, Xu, Bo, Narita, Ichiei, and Yamamoto, Tadashi
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GENE expression ,KIDNEY glomerulus ,TIGHT junctions ,PUROMYCIN ,MESSENGER RNA ,CELL membranes ,POLYMERASE chain reaction ,MASS spectrometry ,IN situ hybridization ,LABORATORY rats - Abstract
Tight junctions are the main intercellular junctions of podocytes of the renal glomerulus under nephrotic conditions. Their requisite components, claudins, still remain to be identified. We have measured the mRNA levels of claudin subtypes by quantitative real-time PCR using isolated rat glomeruli. Claudin-5 was found to be expressed most abundantly in glomeruli. Mass spectrometric analysis of membrane preparation from isolated glomeruli also confirmed only claudin-5 expression without any detection of other claudin subtypes. In situ hybridization and immunolocalization studies revealed that claudin-5 was localized mainly in glomeruli where podocytes were the only cells expressing claudin-5. Claudin-5 protein was observed on the entire surface of podocytes including apical and basal domains of the plasma membrane in the normal condition and was inclined to be concentrated on tight junctions in puromycin aminonucleoside nephrosis. Total protein levels of claudin-5 in isolated glomeruli were not significantly upregulated in the nephrosis. These findings suggest that claudin-5 is a main claudin expressed in podocytes and that the formation of tight junctions in the nephrosis may be due to local recruitment of claudin-5 rather than due to total upregulation of the claudin protein levels. [ABSTRACT FROM AUTHOR]
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- 2011
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15. Identification of potential therapeutic targets in hypertension-associated bladder dysfunction.
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Yono, Makoto, Yoshida, Masaki, Yamamoto, Yasuhiro, Imanishi, Aya, Fukagawa, Atsushi, Latifpour, Jamshid, and Eto, Masatoshi
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HYPERTENSION , *THERAPEUTICS , *GENE expression , *DOXAZOSIN , *POLYMERASE chain reaction , *MESSENGER RNA , *RATS - Abstract
OBJECTIVE To investigate differential gene expression profiles in the bladder of spontaneously hypertensive rat (SHR), as the underlying mechanisms involved in hypertension-associated bladder dysfunction remain to be clarified. MATERIALS AND METHODS SHR and normotensive Wistar-Kyoto (WKY) rats were distributed initially in three groups: group 1 received doxazosin (30 mg/kg/day); group 2 received nifedipine (30 mg/kg/day); and group 3 received the vehicle orally for 4 weeks. The alterations in gene expression levels of candidate genes identified by microarray analysis with potential biological relevance were verified by real-time reverse transcription-polymerase chain reaction (RT-PCR). RESULTS Voiding frequency was significantly higher, and mean voided volume was significantly lower in untreated SHRs than untreated WKY rats. Microarray analysis revealed that 25 of the differentially expressed genes in untreated SHRs compared to untreated WKY rats were related to Gs, Gi, Gq and G12/13 signalling, calcium handling, ion transport and smooth muscle-related genes. Furthermore, RT-PCR data, in accord with the microarray analysis, indicated that untreated SHRs had lower mRNA expression levels of Adcy2, Adcy3, Rgs2, Rgs3, Rgs4 and Arhgdia, and higher mRNA expression levels of Arhgef1, Arhgef11, Arhgef12, Geft, Rock1 and Rock2 than untreated WKY rats. The differential alterations in the micturition patterns and in the expression of several genes related to G-protein signalling pathway observed in SHRs were attenuated by treatment with doxazosin, but not nifedipine. CONCLUSION Our data suggest that differential alterations in the expression of several genes related to Gs, Gq and G12/13 signalling pathways in the SHR bladder might be important in hypertension-associated bladder dysfunction. [ABSTRACT FROM AUTHOR]
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- 2010
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16. Effect of polyphosphoric acid pre-treatment of titanium on attachment, proliferation, and differentiation of osteoblast-like cells (MC3T3-E1).
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Maekawa, Kenji, Yoshida, Yasuhiro, Mine, Atsushi, Van Meerbeek, Bart, Suzuki, Kazuomi, and Kuboki, Takuo
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CELL proliferation , *CELL growth , *GENE expression , *MESSENGER RNA , *POLYMERASE chain reaction , *ALKALINE phosphatase , *EXTRACELLULAR matrix proteins , *GENETIC regulation - Abstract
Purpose: To evaluate the effect of polyphosphoric acid (PPA) pre-treatment of titanium (Ti) on the initial attachment, proliferation, and differentiation of mouse osteoblast-like cells (MC3T3-E1). Materials and methods: Adsorption of PPA to Ti was achieved by immersing Ti disks (15 mm in diameter) into 0, 1, and 10 wt% PPA and 10 wt% orthophosphoric acid (OPA) for 24 h. On each pre-treated Ti disk, 5.0 × 104 MC3T3-E1 cells were seeded, and 1, 3, and 5 h later cell attachment was evaluated. Cell proliferation was also determined 1, 3, and 5 days after cell seed. Cell differentiation was evaluated 5, 10, and 15 days after cell seed using osteoblast marker gene expression. Total RNA was purified from each culture and Type-I collagen, alkaline phosphatase, and osteocalcin mRNA expression levels were measured by real-time reverse transcription polymerase chain reaction. Results: Adsorption of PPA or OPA to Ti significantly accelerated initial cell attachment at every time point ( P<0.0001). As with cell attachment, cell proliferation was also accelerated on the PPA-treated Ti disks in a dose-dependent manner at every time point ( P<0.0001). However, OPA-treated Ti disks did not enhance the cell proliferation. Regarding osteoblastic differentiation, PPA-treated Ti significantly accelerated the Type-I collagen gene expression at 5 and 10 days after cell seed. Regarding alkaline phosphatase and osteocalcin gene expression, no significant difference was found among the different Ti surface conditions. Conclusion: The accelerated cell behavior following Ti pre-treatment with PPA is a promising new strategy to fabricate bioactive Ti implants. [ABSTRACT FROM AUTHOR]
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- 2008
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17. Role of hippocampal TLR4 in neurotoxicity in mice following toluene exposure
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Win-Shwe, Tin-Tin, Kunugita, Naoki, Yoshida, Yasuhiro, and Fujimaki, Hidekazu
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HIPPOCAMPUS (Brain) , *NEUROTOXICOLOGY , *TOLUENE , *CELL receptors , *IMMUNOHISTOCHEMISTRY , *MESSENGER RNA , *GENE expression , *POLYMERASE chain reaction , *LABORATORY mice - Abstract
Abstract: The present study was designed to investigate the possible involvement of TLR4 pathway in the mouse hippocampus following toluene exposure. Male C3H/HeN and C3H/HeJ (TLR4 defective) mice were exposed to 0, 5, 50 or 500ppm of toluene for 6weeks. The expressions of TLR4-related signal transduction pathway mRNAs in the hippocampi were examined using real-time RT-PCR and an immunohistochemical analysis. In C3H/HeN mice, the relative mRNA expression levels of TLR4 and NF-κB activating protein were significantly up-regulated in the groups exposed to toluene, but not in the C3H/HeJ mice. Heat shock protein 70, a possible endogenous ligand for TLR4, mRNA was increased in the C3H/HeN mice exposed to toluene. This is the first report to show that TLR4 may have a role in the neurotoxic effects in mice exposed to toluene. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
- View/download PDF
18. Does advanced-stage endometriosis affect the gene expression of estrogen and progesterone receptors in granulosa cells?
- Author
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Karita, Masako, Yamashita, Yoshiki, Hayashi, Atsushi, Yoshida, Yoko, Hayashi, Mika, Yamamoto, Hikaru, Tanabe, Akiko, Terai, Yoshito, and Ohmichi, Masahide
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ENDOMETRIOSIS , *GENE expression , *ESTROGEN receptors , *PROGESTERONE receptors , *MESSENGER RNA , *HUMAN in vitro fertilization , *SOMATIC cells , *POLYMERASE chain reaction - Abstract
Objective: To evaluate how endometriosis affects the expression of estrogen and progesterone receptors mRNA in granulosa cells.Design: Prospective study.Setting: IVF-ET program at Osaka Medical College.Patient(s): Eighteen patients with revised American Fertility Society stage IV endometriosis and 23 patients with tubal infertility without endometriosis.Intervention(s): Granulosa cells obtained from patients with endometriosis were examined for estrogen (ER-β, ER-α) and progesterone (PR-A, PR-B) receptor mRNA expression ratio against GAPDH.Main Outcome Measure(s): Hormonal environment and clinical outcome were investigated. Expression of ER and PR mRNA were evaluated by StepOne real-time polymerase chain reaction (PCR) analysis.Result(s): Total hMG/FSH levels were statistically higher in patients with endometriosis; however, high-quality embryo rates and pregnancy rates were lower in patients with endometriosis than in patients with tubal infertility. Expression of PR-A and ER-α in patients with endometriosis was statistically higher than in patients with tubal infertility. The expression of PRs and ERs in patients with tubal infertility showed a positive correlation; however, it was not identified in the endometriosis group.Conclusion(s): The higher PR-A and ER-α gene expression in granulosa cells in patients with endometriosis, compared with patients with tubal infertility, might be a leading cause of ovarian dysfunction due to endometriosis. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
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