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In-depth research on key technologies of ultra-low temperature valves has far-reaching impact on improving special valve technologies in China. When the temperature distribution of the ultra-low temperature valve was simulated, the analysis method used ignored the heat transfer between the low-temperature gas and the valve after the low-temperature medium absorbed the heat and underwent phase change. Therefore, taking Class300-DN65 ultra-low temperature cut-off valve as an example, the influence of medium state change and low temperature gas produced by phase change on valve cooling effect during steady-state operation was studied. Firstly, computational fluid dynamics（CFD） simulation of the medium flow process under adiabatic condition was performed, and a heat transfer model was established to explain the heat transfer process. Then, when heat transfer occurred, the internal clearance of the valve cover and the media distribution law of the flow field inside the valve were studied. Finally, the temperature distribution of the valve was analyzed by steady-state flow-thermal coupling. The research results show that the low temperature gas generated by evaporation and vaporization of LNG will flow at the bottom of the internal cavity of the valve cover, and constantly cool down the valve cover and stem by convective heat exchange, and the bottom temperature of the stuffing box is 10.6 ℃ when the effect of low temperature gas on the temperature field of the valve structure is not considered; but the bottom temperature of the stuffing box is only-0.2 ℃ when the low temperature gas is considered. Therefore, when designing the ultra-low temperature valves, it is necessary to consider the effect of cryogenic gas on the cooling of the valve, which otherwise may lead to seal failure. [ABSTRACT FROM AUTHOR]

Chronic kidney disease (CKD) is a common chronic disease in clinic, which seriously affects the quality of life and economic burden of patients.Traditional treatments such as drugs, kidney dialysis and kidney transplantation cannot reverse the progress of CKD at present.In recent years, stem cell therapy, as a new type of cell therapy, has achieved remarkable results in the treatment of CKD.This article reviews the research progress of stem cells in CKD,and provides a new basis for the treatment of CKD. [ABSTRACT FROM AUTHOR]

口腔内科疾病是影响人们口腔健康的主要因素之一，治疗该疾病的方法种类繁多，其中氢氧化钙成为近 年来备受关注的一种治疗方法。本文对氢氧化钙在社区口腔内科疾病治疗中的应用进行系统的综述，内容包括氢氧化 钙概述、氢氧化钙在口腔内科疾病治疗中的应用、氢氧化钙的优点和不足、氢氧化钙在口腔内科疾病治疗中的研究进 展等方面。研究结果表明，氢氧化钙具有广泛的应用价值，可以有效治疗口腔内科疾病，但也存在一些不足之处，需 要进一步的研究和改进。 [ABSTRACT FROM AUTHOR]

Objective To establish an automatic review system for blood coagulation test report and evaluate its application effect. Methods　The rules for the examination of coagulation test reports were written into Lis Procedure. A total of 6 281 reports that were automatically audited during the commissioning period were selected for manual verification, to evaluate the feasibility of the rules. Results The passing rate of automatic verification was 71.34%, False positive rate (4.49%), False negative rate (0.032%), Positive coincidence rate (24.14%), Negative coincidence rate ( 71.34%), Consistent rate ( 95.48%) and correct passing rate ( 99.96%). Conclusion There are 23 intercepting rules, the Delta Check rule of APTT needs to be improved, and the rest of 22 intercepting rules’ correct passing rate is 100%. [ABSTRACT FROM AUTHOR]

Agricultural green production is the fundamental way to ensure the quality and safety of agricultural products and alleviate the deterioration of China􀆳s ecological environment. Although academia has conducted abundant research on the factors affecting farm⁃ ers' green production behavior, little is known about the relationship between agricultural product e-commerce and fruit farmers' green production. Therefore, this article explored the relationship between E-commerce participation and fruit farmers' green production be⁃ havior and its impact mechanism. Then, based on 812 field survey data collected from four counties of three provinces, the RBP model was used for an empirical test. Finally, the robustness and heterogeneity tests were performed. And the following conclusions were drawn: ① E-commerce participation could improve the probability of fruit farmers' green production implementation, and the average treatment effect of the treated (ATT) was 24.7%. Moreover, the above benchmark conclusions were robust and reliable after robust dis⁃ cussions through methods such as replacing models and replacing explanatory variables. ② There were differences in the treatment ef⁃ fects of E-commerce participation on different green production behaviors, and these differences were shown in descending order ac⁃ cording to the value of ATT as: strictly following the standard pesticides application, physical control measures, and biological control measures. ③ E-commerce participation could encourage fruit farmers to implement green production behaviors by enhancing green pro⁃ duction awareness, raising agricultural product price expectations, and pursuing a positive consumer reputation. Therefore, it is neces⁃ sary to increase the publicity and promotion of agricultural product E-commerce, thereby increasing the participation of fruit farmers in E-commerce. Meanwhile, it is also necessary to ensure the effectiveness of agricultural product E-commerce in improving the quality of agricultural products and obtaining a quality premium by implementing green production so as to promote the synergistic improvement of the participation rate of E-commerce and the implementation rate of green production. In addition, the guiding role of the government in green production should be strengthened. [ABSTRACT FROM AUTHOR]

目的: 研究牙齿正畸医治治疗方法对牙周病致前牙移位的临床治疗效果. 方法: 选择2020年1月-2021年 12月来我院就诊 的90例牙周病致前牙移位患者做为研究主体. 依照性别、年龄平衡相匹配的标准分成观察组45例和对 照组45例. 对照组进行基础牙周炎治疗, 观察组执行牙齿正畸干预. 比较2组患者术后牙齿松动情况、牙周指标、生 活品质、菌斑指数、牙龈指数牙松度的差别. 结果: 对其牙周病致前牙移位患者执行牙齿正畸术后, 观察组患者的牙 松情况较对照组获得了有效缓解, 差别有统计学意义 (P<0.05 ); 观察组患者前牙覆牙合、覆盖、牙周袋深度均好 于对照组, 差别有统计学意义 (P<0.05 ), 但2组患者牙槽相对高度相比, 差别不大无统计学意义 (P>0.05 ); 观察 组患者生活品质 (咀嚼功能、美观性、整体感受身心健康) 得分均好高于对照组, 差别有统计学意义 (P<0.05 ); 观察组菌斑指数、牙牙龈指数、牙松度均显著小于对照组, 差别有统计学意义 (P<0.05 ). 结论: 对牙周病致前牙 移位患者采取执行牙齿正畸术后, 可有效缓解患者的临床表现, 提高患者的生活品质. [ABSTRACT FROM AUTHOR]

原发性肝癌是严重威胁人类健康的恶性肿瘤之一， 肝移植是肝癌的重要治疗方法， 但术后高复发转移率 严重影响其治疗效果。随着分子生物学的发展， 研究发现了许多与肝癌肝移植术后复发转移相关的分子生物标志 物， 如某些酶类、生物蛋自类、分化群分子类及肿瘤复发转移相关基因、RNA 等。对肿瘤组织内与肝癌复发转移相 关的分子生物标志物进行归纳总结，并分析其预测价值， 对具有复发转移倾向的肝癌肝移植患者进行有效千预， 对 提高治疗效果、改善预后具有非常重要的临床意义. [ABSTRACT FROM AUTHOR]

In order to comply with the reform of the training mode of innovation talents in electronic information， cultivate innovative talents under the background of new engineering subjects, meet the needs of innovative talents of society，combined with the teaching characteristics of the school，this paper puts forward the exploration and practice of the training mode of electronic information innovation talents with competition as the carrier, and formulates innovation. It also reconstructs the engineering practice platform，rebuilds the teaching system，improves and perfects the innovative talent training mechanism which based on curriculum reform, taking subject competition as carrier and relying on collaborative education mechanism. Furthermore, it improves the quality of cultivating innovative talents with innovative consciousness, spirit and ability. [ABSTRACT FROM AUTHOR]

Gadolinium-ethoxybenzyl-diethylenetriamine pentaacetic acid( Gd-EOB-DTPA) is a hepatobiliary-specific magnetic resonance imaging( MRI) contrast agent and has been widely used in clinical practice. With reference to the expert consensus and references in China and foreign countries,the experts in China obtained plenty of scan experience in clinical practice,optimized,supplemented,and refined the scan regimen of Gd-EOB-DTPA-enhanced MRI,and thus reached a consensus on intended population,contrast agent injection,scan regimen,and quality control,in order to standardize the regimen of Gd-EOB-DTPA-enhanced MRI and help with imaging diagnosis and the development of clinical treatment strategies. [ABSTRACT FROM AUTHOR]

Digital plant is the key of technology for agricultural digitization and informatization, and it has become a hot research field both at home and abroad. This technology can analyze the morphological structure and growth process of plants with the aid of the digital and visual three-dimensional (3D) model. The technology can analyze growth and development status for plants and evaluate the type characteristics of plants in real time, and hence guide agricultural production. It can provide powerful conditions for agricultural mechanization and automation production. Obtaining 3D point cloud data of plants is the difficulty and key for the plant digital and visual modeling. Domestic and foreign research focused on 3D laser scanning to obtain 3D point cloud data of plant, and achieved some results. High-precision 3D laser scanning can obtain accurate 3D point cloud data for the reconstruction of plants and realize the digitization of plants, but this method needs expensive equipment. This processing algorithm is complex and has poor real-time performance, because of a large amount of data. This method is unfavorable to the application and popularization. To design a low cost and good practicability method for obtaining plant 3D point cloud data had important research value and significance. TOF (time of flight) depth sensor was a new method for depth information acquisition based on the principle of time of flight. The method could quickly acquire the depth information of the image, the depth calculated was not affected by surface gray level and characteristics, and the calculation accuracy did not change with distance. There were few researches on the application of TOF depth sensor in plant digitalization and visualization, and the research field abroad was just in its infancy. In order to improve the accuracy of 3D reconstruction for plants and realize the digitization of plants, the obtaining and denoising method of plants 3D point cloud data was proposed based on TOF depth sensor. This method possessed the advantages of low cost and good practicability. Firstly, the plant point cloud data were obtained through TOF depth sensor, and then pretreated by direct pass filter in order to reduce the background noise. Secondly, the improved outlier denoising algorithm based on density analysis was combined with neighboring average distance and neighborhood point number, and these were 2 characteristic parameters. The algorithm of outlier could detect and denoise the outliers of point cloud data. Finally, small-size noise in the point cloud was detected and denoised by bilateral filtering algorithm. Tomato plants were selected to carry out experiment. Compared with the traditional bilateral filtering algorithm, experiments showed that the maximum error was reduced by 11.2%, and the average error was reduced by 23.2%. Compared with the Laplace filtering algorithm, experiments showed that the maximum error was reduced by 20.6%, and the average error was reduced by 39.2%. It showed that the proposed algorithm could simply and effectively denoise noise with different scales in 3D point cloud data under the condition of keeping the characteristics of point cloud. The point cloud data are large for 3D reconstruction process, and therefore the artificial intelligence method can analyze the point cloud data in the further study. [ABSTRACT FROM AUTHOR]

目的：调查医务工作者职业相关肌肉骨骼疼痛的患病现状, 分析其相关因素, 为临床干预提供依据。 方法：采用横断面调查方法, 应用自制《肌肉骨骼疼痛调研表》, 于2020年7—9月对上海市2家三级医疗机构、2家二 级医疗机构和4家一级医疗机构的1421名医护工作者进行了问卷调查。 结果：医务工作者普遍存在颈肩腰下肢痛等肌肉骨骼疼痛, 尤以颈痛和腰痛常见, 但大部分症状较轻；相关分析发现 这些疼痛均与生存质量相关。医务工作者职业相关肌肉骨骼疼痛均随执业年限的增加而增加, 并与性别、糖尿病、 骨质疏松等内分泌代谢疾病的发病相关。 结论：医务工作者职业相关肌肉骨骼疼痛广泛存在, 严重影响医务工作者的生存质量。由于症状大部分较轻, 提示 康复干预是其重要的治疗措施。康复干预时不仅要重视局部的治疗, 而且要注意性别的差异, 同时需要关注患者的 内分泌代谢疾病的防治。 [ABSTRACT FROM AUTHOR]

[Objective] To explore the potassium chloride application measures suitable for Luzhou tobacco planting area and further improve the regulation technology of upper tobacco leaves in the field. [Method] A field experiment was conducted with Zhongchuan 208' as the test material. Four potassium chloride application rates of 30,60,90 and 0 kg/hm² were set. The ratio of base fertilizer to topdressing was 5: 5. The topdressing period was set at 30 and 50 d after transplanting, with a total of seven potassium chloride application methods. The changes of agronomic characters, SPAD value, economic characters, chemical composition and physical characters of upper tobacco leaves were analyzed. [Result] Compared with no application of potassium chloride, the application of potassium chloride increased the yield, output value, average price and the proportion of medium and high-grade tobacco by 3.11%-9.17%,6.12%-19.29%, 2.79%-12.92% and 4.01%-18.25% respectively. The color, maturity, body, oil content and chroma of flue-cured tobacco were improved, and the comprehensive score was increased by 1.15%8.30%. [Conclusion] Applying 30 kg/hm² potassium chloride as base fertilizer and applying 30 kg/hm² after transplanting for 50 days has the highest economic benefit. The appearance quality, chemical composition and physical properties of the upper tobacco leaves after baking get the highest score. [ABSTRACT FROM AUTHOR]

Coronavirus infection seriously threatens human health. There is no specific medication or vaccine so far. In recent years, domestic and foreign researchers have developed a variety of small-molecule inhibitors against the ligand S protein, RdRp, PLpro and 3CLpro of three highly pathogenic coronavirus, SARS-CoV， MERS-CoV， SARS-CoV-2. This article reviews the characteristics of these coronaviruses, action targets, small molecule inhibitors, and structure-activity relationships. [ABSTRACT FROM AUTHOR]

Objective: To search the hotspots and research trend of premature birth by bibliometric analysis, so as to propose the new directions for further research. Methods：Studies about Premature Birth in PubMed were searched from the establishment to April 2019. Bibliographic Items Co-Occurrence Matrix Builder (BICOMB) was used to extract the retrieved documents. High-frequency MeSH terms were identified to form article Matrix and co-occurrence Matrix. Hierarchical cluster analysis was conducted by SPSS 20.0 to form the hotspots. The strategic diagram was then created. Results：A total of 2 965 papers were retrieved, and 3 183 main MeSH terms were extracted by BICOMB software. Sixty four high-frequency keywords were determined and divided into six relevant research hotspots. Finally, a strategic diagram reflecting the development trend of each research hotspot was formed. Conclusions：The pregnant complications and comorbidities, adverse outcomes of maternal and child caused by premature birth, maternal body mass index, gestational age and parity were well developed as the relevant research hotspots. On the contrary, the factors of society, psychologyand genetics, and the adverse exposure during pregnancy, were not adequately documented, suggesting that these factors could be studied in future. In addition, the researches on the diagnosis and prevention of preterm birth are relatively mature, but the connection with other studies is not close enough and further development is needed. [ABSTRACT FROM AUTHOR]

【Objective】To solve the problem of large differences in the maturity status of the upper leaves of flue-cured tobacco at one-time harvest, the present experiment explored the effects of gibberellin and potassium nitrate spraying concentration and spraying time on the simultaneous maturation and flue-cured tobacco quality of the upper 6 tobacco leaves of the flue-cured tobacco Zhongchuan 208. The impact was expected to provide a theoretical basis for promoting the one-time harvest of flue-cured tobacco. 【Method】Using the Box-Behnken method in response surface optimization design, the concentration of gibberellin (0, 15, 30 μmol/L) and potassium nitrate (0, 0.5, 1.0 g/L) and the spraying time (20, 25, 30 days) after topping was designed with three factors and three levels, a total of 16 treatments. The physiological indicators related to the maturation of the 1-3 tobacco leaves from top to bottom(denoted as Tup) and the 4-6 tobacco leaves from top to bottom(denoted as Tdown) were measured, and the treatment indexes were calculated. The synchronization maturity value was used as a basis for measuring synchronization maturity. The smaller the value, the better the synchronization maturity state. 【Result】 The synchronous maturity value of each treatment was significantly lower than that of the control, and the appearance quality and total chemical composition of cured tobacco leaf were higher than that of the control. The treatment of spraying 15 μmol/L gibberellin and 0.5 g/L potassium nitrate (T2, T5, T9, T16) had the best synchronous mature state, and the highest total score of appearance quality and chemical composition; The results of response surface showed that gibberellin and potassium nitrate had a very significant impact on the comprehensive score, and the impact curve was similar. The spraying time also had a very significant impact on the comprehensive score. The model validation and optimization results showed that the comprehensive score of flue-cured tobacco was close to the predicted value, indicating that the model was reliable and could predict the comprehensive score of flue-cured tobacco.【Conclusion】Spraying gibberellin and potassium nitrate can promote the uniform maturity of upper leaves and improve the quality of flue-cured tobacco leaves. [ABSTRACT FROM AUTHOR]

Aiming at the problems of test case reduction (TCR) technology based on intelligent algorithms that it is easy to fall into local convergence and premature maturity, this paper proposed a test case set reduction algorithm based on per-survivor processing (PSP). The algorithm used the sequential grid search idea of PSP algorithm to binary code the test case set to form the state space of sequential grid search. In the state transition stage, the algorithm took the code coverage and the effective execution time of test cases as branch metrics. According to the calculation results, the path with the largest branch weight was the surviving path, so as to eliminate redundant states and complete the reduction of test case set. The experimental results show that under the same experimental environment, compared with other algorithms, this algorithm not only has higher redundancy rate (RR), but also ensures higher error detection rate (EDR). This algorithm reduces the complexity of software testing to a certain extent and improves the efficiency of software testing. [ABSTRACT FROM AUTHOR]

Objective Fragmented red blood cells (FRC) is a new detection parameter provided by the automatic blood cell analyzer, which is closely related to thrombotic microangiopaemia and other diseases. This study is aimed at evaluating the detection performance of automatic hematology analyzer for RBC fragments. Methods The performance of SYSMEX XN series automatic blood cell analyzer was evaluated by carrying contamination rate, precision, linearity, accuracy, sensitivity, specificity and other indicators. All data were analyzed by SPSS 19.0 software. Results The contamination rate of red blood cell fragments detected by SYSMEX XN series automatic hematology analyzer was 0.11%. Intra-batch precision: coefficient of variation (CV) at each experimental concentration were 8.75% (42.15×109/L), 6.38% (83.42×109/L), 4.57% (228.74×109/L), 5.31% (796.89×109/L). Linear verification: the linear regression equation was Y = 1.000 1X + 9.328 8, and the correlation coefficient is r = 0.9936., and the correlation coefficient is R =0.993 6; Correctness verification: compared with manual microscopy, the was no statistically significant difference(P＞0.05), the correlation coefficient, sensitivity and specificity were r = 0.82, 92% and 82% respectively. Conclusion SYSMEX XN series automatic blood cell analyzer is quick and easy to operate in detecting red blood cell fragments, with low contamination rate, fair precision and satisfactory linear range. Compared with manual microscopy, it has better correlation, higher specificity and sensitivity. Blood cell analyzer can be used as a rapid screening method for fragments of peripheral blood red blood cells, but the precision of its detection still needs to be further improved, and due to the limitations of the method, positive specimens need to be confirmed under a microscope. [ABSTRACT FROM AUTHOR]

Objective: To compare the efficacy of hysteroscopic cold knife separation and electrotomy in the treatment of intrauterine adhesion (IUA) and its effect on the recovery of intrauterine morphology and serum interleukin. Methods: The clinical data of 83 patients with IUA who were treated in our hospital from April 2019 to February 2021 were analyzed retrospectively. According to the different operation methods, the patients were divided into group A (electrosurgical resection under hysteroscopy, 40 cases) and group B (cold knife separation under hysteroscopy, 43 cases). The operation time and hospital stay, the recovery of uterine morphology and the changes of serum interleukin were compared between the two groups. The incidence of postoperative complications, the improvement rate of menstruation and the incidence of uterine re adhesion were observed. Results: The operation time and hospital stay in group B were shorter than those in group A(P<0.05). The total effective rate and satisfactory rate of epithelial healing of intimal wound in group B were higher than those in group A(P<0.05). The levels of serum interleukin-6 (IL-6) and interleukin-8 (IL-8) in group B were higher than those in group A (P<0.05). The levels of serum interleukin-4 (IL-4) and interleukin-10 (IL-10) in group B were lower than those in group A (P<0.05). There was no significant difference in the incidence of postoperative complications between the two groups (P>0.05). The improvement rate of menstruation in group B was higher than that in group A, and the incidence of uterine re adhesion was lower than that in group A(P<0.05). Conclusions: Compared with hysteroscopic electrotomy in the treatment of IUA, hysteroscopic cold knife separation in the treatment of IUA has shorter operation time and hospital stay, better recovery of uterine morphology and improvement of menstruation, milder inflammatory reaction, lower incidence of uterine re adhesion and better curative effect. [ABSTRACT FROM AUTHOR]

骨髓间充质干细胞(BMSCs)移植治疗急性肾损伤(AKI)､慢性肾脏病(CKD)、糖尿病肾病(DN)及其 他肾脏疾病均显示出一定效果,但BMSCs 归巢数量､在靶器官或靶组织受损组织)中的分布及归巢后BMSCs的 存活率等,都是 BMSCs治疗肾脏疾病效果的影响因素。如何提高 BMSCs 向受损组织移动的能力是提高治疗效 果的关键所在。现阶段大部分基础实验在如何增加 BMSCs 向 AKI､CKD 和 DN 等动物模型的损伤肾脏归巢进行 了报道。研究表明,提高血管内皮细胞基质细胞衍生因子1的表达、BMSCs趋化因子受体 CXCR4 的表达､改变 损伤组织微环境及超声手段等,均能提高 BMSCs 的归巢能力,达到提高对肾脏疾病治疗效果的目的,并可减少 BMSCs 在其他组织中聚集引起的并发症。但如何提高归巢之后 BMSCs 的存活率､治疗效果及持续时间等问题 有待进一步研究。 [ABSTRACT FROM AUTHOR]

Objective To observe the therapeutic effect of bone marrow mesenchymal stem cells (BMSCs) transplanted by different ways on chronic kidney disease (CKD) rats and the effect of nucleotide binding oligomerized domain-like receptor protein 3 (NLRP3) inflammasome on renal fibrosis. Methods There were 50 male SD rats in this study, of which 2 were used to isolate and culture BMSCs in vitro. After successful CKD modeling (left nephrectomy+multiple tail vein injection of doxorubicin), thirty-six rats were randomly divided into following groups: the model group (CKD group), the renal artery transplantation of BMSCs group (A-M group) and the tail vein transplantation of BMSCs group (V-M group). The normal control group (SHAM group, n=12) was given 0.9%NaCl by caudal vein infusion. The serum levels of creatinine, urea nitrogen and 24 h urinary protein were measured at 7 d and 14 d after BMSCs transplantation. HE staining and Masson tricolor staining were performed to observe the pathological structure changes and fibrosis of the kidney. Immunohistochemical staining was performed to observe the expression levels of NLRP3, apoptosis-related microprotein (ASC) and cysteine aspartate proteinase-1 (Caspase-1). Results Compared with SHAM group, the serum creatinine, urea nitrogen and 24 h urinary protein were significantly increased on day 7 and day 14 in the CKD group, A-M group and V-M group (P＜0.05). On 7 and 14 days after BMSCs transplantation, the serum creatinine, urea nitrogen and 24 h urinary protein were significantly lower in the A-M group and the V-M group than those in the CKD group, and the A-M group was lower than those in the V-M group (P＜0.05). HE staining and Masson staining showed that the inflammatory infiltration, the number of atrophic glomeruli, the dilatation of renal tubules and the degree of fibrosis were reduced in the A-M group and V-M group compared with those of the CKD group. The improvement degree in the A-M group was better than that in the V-M group. Immunohistochemical results showed that the expression levels of NLRP3, ASC and Caspase-1 in renal interstitium of rats were decreased in the A-M group and V-M group compared with those in the CKD group, and the decrease was better in the A-M group than that in the V-M group. Conclusion BMSCs can improve the renal fibrosis in CKD rats. The arterial approach is better than the venous approach in the observation period. Its mechanism may be related to the inhibition of NLRP3 inflammasome activation. [ABSTRACT FROM AUTHOR]

Objective　To synthesize and investigate cytotoxicity of an indole-chalcone derivative FC58. Methods　The target compound was synthesized through the Aldol condensation with 1-(3,4,5-trimethoxyphenyl)ethan-1-one and 1H-indole-3-carbaldehyde. The Cell Titer-Blue method was used to determine in vitro cytotoxicity. The cell cycle experiment was performed to analyze the action characteristics of FC58. Results　FC58 exhibited high cytotoxicity against various leukemia cells and resulted in G2/M phase arrest. It showed stronger drug resistant index than traditional tubulin inhibitors such as paclitaxel, vinblastine and doxorubicin. Conclusion　FC58 represents a promising lead compound for multi-drug resistant leukemia. [ABSTRACT FROM AUTHOR]

Objective To investigate the P38MAPK-mediated mitochondrial damage induced by TαPcZn-PDT in Lovo cells. Methods Lovo cells were divided into control group, siRNA-negative group, siRNA-P38MAPK group, TαPcZn-PDT group and TαPcZn-PDT/siRNA-P38MAPK group. The cells in each group were treated and irradiated by red light for 10 minutes and then incubated for 3 hours. After incubation, RT-PCR and Western blot assay were used to detect the effect of p38MAPK silencing. The cell ROS levels were detected by DCFH-DA probe. JC-I/7-AAD double-labeled staining was used to detect changes of cell mitochondrial membrane potential (ΔΨm). Annexin V-FlOUS/7-AAD doublelabeled staining was used to detect the cell apoptotic rate. Results After siRNA interference, the protein expressions of P38MAPK mRNA significantly decreased. TαPcZn-PDT induced the increase of ROS, the depolarization of mitochondrial ΔΨm and the apoptotic rate. After siRNA silencing P38MAPK, the ROS was decreased, the number of depolarized cells was decreased and the apoptosis was weakened, which was induced by TαPcZn-PDT in Lovo cells. Conclusion That silencing of P38MAPK can significantly inhibit the production and release of ROS and decrease the depolarization of mitochondrial ΔΨm, thus attenuate the apoptosis induced by TαPcZn-PDT. [ABSTRACT FROM AUTHOR]

Objective: To investigate the efficacy of phacoemulsification and small incision extracapsular cataract extraction in the treatment of cataract and their effects on corneal endothelial cells and quality of life. Methods: 203 patients with cataract who were treated in our hospital from March 2015 to June 2017 were selected, according to the random number table method, the patients were divided in­to the control group with 103 cases and the observation group with 100 cases. The control group was treated with small incision extracap­sular cataract extraction, and the observation group was treated with phacoemulsification. The visual acuity of all patients was measured before operation, 1 weeks after operation, 1 months after operation and 3 months after operation. The number of corneal endothelial cells was measured before and 2 weeks after operation, and the cell density and hexagonal cell ratio were calculated. The quality of life was evaluated before and 1 months after operation, and the complications were recorded. Results: There was no significant difference in visual acuity between the two groups before operation, 1 months after operation and 3 months after operation (P>0.05), the visual acuity of the observation group was significantly higher than that of the control group 1 week after the operation (P<0.05). At 2 weeks after operation, the cell density and hexagonal cell ratio of the two groups were decreased, and the cell density of the control group was lower than that of the observation group(P<0.05). There was no significant difference in scores of self-care ability, activity ability, social ability and psycho­logical emotion between the two groups before and 1 months after operation(P>0.05), the scores of self-care ability, activity ability, social ability and psychological emotion in the two groups 1 months after operation were higher than those before operation(P<0.05). There was no significant difference in the incidence of complications between the two groups (P>0.05). Conclusion: Compared with small incision extracapsular cataract extraction, phacoemulsification can improve the vision of patients faster in the short term, and it has less damage to the corneal endothelial cells, however, the short-term effects and complications of the two operations are basically the same, and there is no difference in the quality of life of the patients. [ABSTRACT FROM AUTHOR]

Objective To explore the role of respiratory rate change in regulating the balance of cardiovascular autonomic nerve and its influence on arrhythmia after myocardial ischemia. Methods Seventeen standard laboratory rats including 9 in normal group and 8 in myocardial ischemia model group were conducted with mechanical ventilation by ventilator to change respiratory rates. The fundamental tidal volume was constantly set at 10 mL/kg while respiratory rate changed(increasing by 10% and 20%, and decreasing by 10% and 20% of fundamental respiratory rate). Blood pressure and heart rate were recorded continuously; the influence of respiration rate regulation on heart rate variability(HRV) and blood pressure instant variability(BPIV) was analyzed; the synchronism of respiration rate with changes of heart rate and blood pressure was observed; the effect of reduced respiration rate on HRV in the two groups was evaluated. Results The cyclical changes of RR interval and blood pressure coincided with respiratory rate. The central frequencies of HRV and BPIV were both consistent with that of respiratory power spectral density. The variation trend of respiratory rate was essentially in agreement with that of heart rate and blood pressure, and especially, the variation trend of heart rate and blood pressure was completely identical. Conclusion HRV and BPIV under respiratory regulation correspond with physiological mechanism of respiratory sinus irregularity. It is the secondary effect of RR interval changes and reflects the results of autonomic nervous balance. The frequency of heart beat contributes to the synchronous changes of heart rate and blood pressure. It may reduce the incidence of myocardialischemiainduced arrhythmia to slow respiratory rate. [ABSTRACT FROM AUTHOR]

Objective: To analyze the blood rheology and hemodynamics in patients with primary open angle glaucoma and explore its relationship. Methods: 43 patients(n=51 eyes)with primary open angle glaucoma treated in our hospital from January 2015 to June 2016 were collected as case group, at the same time, 25 healthy volunteers(n=50 eyes)were selected as control group. The blood rheology parameters such as fibrinogen, whole blood specific viscosity, plasma ratio viscosity of the two groups were detected, the spectrum of central retinal artery(CRA) and ophthalmic artery(OA) in two groups were detected by color Doppler ultrasound, and hemodynamic parameters such as resistance index(RI), peak systolic velocity(PSV), end diastolic velocity(EDV) were compared between the two groups. Results: The fibrinogen, low shear and high shear whole blood viscosity, plasma ratio viscosity of case group were higher than those of control group, the differences were statistically significant(P<0.05). The RI in CRA, OA of case group was higher than that of control group respectively, and the PSV, EDV in CRA, OA of case group were lower than those of control group respectively, the differences were statistically significant(P<0.05). The fibrinogen, low shear and high shear whole blood viscosity, plasma ratio viscosity of blood rheology in patients with primary open angle glaucoma were positive association with RI of hemodynamics, which were negative association with PSV, EDV of hemodynamics, the differences were statistically significant(P<0.05). Conclusion: There are blood circulation disorder with different degrees in patients with primary open angle glaucoma, joint detection of blood rheology and hemodynamics can be used to be an important indicator for the diagnosing and evaluating prognosis of primary open angle glaucoma. [ABSTRACT FROM AUTHOR]

Objective: To study the efficacy of travoprost eye drops in the treatment of open angle glaucoma and its effect on hemodynamics. Methods: 164 patients with open angle glaucoma who were treated in our hospital from August 2014 to December 2014 were selected as the research object, they were divided into control group (82 cases) and experimental group (82 cases) according to random number table method. The control group was given timolol maleate eye drops, and the experimental group was given travoprost eye drops. Results: The intraocular pressure and visual change of two groups were observed after 12 weeks. The peak systolic velocity (PSV), end diastolic velocity(EDV) and resistance index(RI) of ophthalmic artery (OA), short posterior ciliary artery(SPCA) and central retinal artery (CRA) were detected by color doppler ultrasonic. The adverse reactions were observed. The intraocular pressure and visual acuity in the two groups were compared between before treatment and 2 weeks after treatment, the differences were not statistically significant (P>0.05); After treatment, the intraocular pressure of the patients in the two groups which compared with before treatment was decreased, the vision of the patients in the two groups which compared with before treatment was increased, the intraocular pressure with decreased and the vision with increased in the experimental group were more prominent, the differences were statistically significant (P<0.05); At 6 and 12 weeks after treatment, the intraocular pressure in the experimental group was lower than that in the control group, the vision in the experimental group was higher than that in the control group, the differences were statistically significant (P<0.05). After 12 weeks treatment, the levels of EDV, PSV of OA, SPCA and CRA in experimental group were higher than that in the control group, and the RI of OA, SPCA and CRA in experimental group were lower than that in the control group, the differences were statistically significant(P<0. 05). The adverse reactions were mainly mild foreign body sensation, mild conjunctival congestion, iris pigment deepen in two groups, the incidence of adverse reactions in the two groups were compared, the difference was not statistically significant (P>0.05). Conclusions: Travoprost Eye Drops in the treatment of open angle glaucoma can reduce intraocular pressure, improve eyesight, improve the ocular hemodynamics indexes, and better security. It was worthy of clinical popularization and application. [ABSTRACT FROM AUTHOR]