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Uneven voltage, strong magnetic field and current effect can easily disturb the optimization of distribution network flexibility, The flexibility improvement effect is not obvious. In order to solve problems above, a two-stage flexibility optimization method was proposed for distribution network. Firstly, the instantaneous power balance algorithm was used to ensure the distribution network to reach the three-phase equilibrium. Secondly, the reactive power planning of the distribution network was realized through the output characteristics of reactive power output and active power loss, which effectively improved the operation quality and reduces the network loss. Finally, the two-stage optimization method that integrated diversified electric sources and loads was adopted to improve the flexibility of distribution network. In the first stage, a flexibility optimization model was built based on three indicators of flexibility and the supply characteristics of diversified electric sources and loads. In the second stage, in consideration of probability balance of distribution network flexibility indicators, the model was solved by linear transformation algorithm to complete the flexibility optimization of distribution network. Test results show that the proposed method has the advantages of small loss rate, small fluctuation curve of net load, short calculation time and flexible adaptability of distributed power access in distribution network. It can be seen that the flexibility of distribution network with distributed power supply can be effectively improved and the ability of distribution network to accept distributed power supply can be improved through flexible optimal scheduling. [ABSTRACT FROM AUTHOR]

报道1例在首都医科大学附属北京世纪坛医院内分泌科住院的2型糖尿病合并非酒精 性脂肪性肝炎患者，应用胰岛素强化治疗效果不佳转换为司美格鲁肽联合二甲双胍治疗，在改善糖脂 代谢的同时，改善肝脏脂肪沉积及逆转肝纤维化分级的诊疗过程。患者为 52 岁男性，因多饮、多尿 6 年，血糖控制不佳 2 年入院。患者身高 185 cm，体重 92 kg，体重指数 26.88 kg/m2 ，腰围 98 cm。糖化 血红蛋白 12.1%。入院前使用胰岛素强化降糖治疗，胰岛素用量高达 88 U/d，但空腹血糖仍高达 13~ 15 mmol/L，餐后血糖>20 mmol/L。既往有高血压病、非酒精性脂肪性肝炎病史（血清转氨酶高于正常 上限 2倍，饮酒量<30 g/d）。入院后完善相关检查，明确诊断为 2型糖尿病、2型糖尿病性周围神经病 变、2型糖尿病性肾病Ⅲ期、高血压病3级（很高危组）、高脂血症、非酒精性脂肪性肝炎、肝纤维化。治 疗上予司美格鲁肽0.25 mg、1次/周皮下注射、二甲双胍0.25 g、3次/d口服联合地特胰岛素8 U睡前皮 下注射治疗。治疗90 d后，患者糖脂代谢渐平稳达标，体重指数、腰围下降，肝功能及肝纤维化4项好 转，瞬时弹性超声成像显示肝脏脂肪沉积减少、肝纤维化分期改善，且无明显胃肠道反应，无低血糖 发生。 [ABSTRACT FROM AUTHOR]

Objective To demonstrate the major components and molecular mechanism of Shenling Baizhu San in treating primary osteoporosis by using LC-MS and single-cell RNA sequencing analysis. Methods LC-MS technology was used to identify the components of Shenling Baizhu San and its targets were predicted. The transcriptome and scRNA data related to POP were obtained, the differentially expressed genes (DEGs) and cell subpopulations were analyzed, and weighted gene co-expression network analysis (WGCNA) was further performed on DEGs to obtain the hub genes and construct PPI network. Finally, virtual molecular docking was used to verify the components and core targets. Results After LC-MS identification and screening, 10 components and 414 potential targets were obtained. Differential and WGCNA analysis respectively obtained 843 POP-DEGs and 1976 hub targets, and 19 core targets were obtained. Single-cell sequencing analysis found that Macrophage, Monocyte, T cells, B cellexisted in POP sample. The enrichment results showed that the core targets were involved in cellular senescence, osteoclast differentiation, MAPK signaling pathway. Finally, the virtual molecular docking results showed that the identified components could form a stable docking with the POP core targets. Conclusion Shenling Baizhu San can regulate cell senescence, immune function and osteoclastic differentiation through multiple components, multiple targets, multiple cells and multiple pathways to treat POP. [ABSTRACT FROM AUTHOR]

The YTH domain containing 2 (YTHDC2)protein, a methyl-binding protein of -methyladenosine (m"A), recognizes and binds mbA modifications to mediate the biological functions of mbA modifications. As mbA modifications are widely found on various RNAs, the biological functions of YTHDC2 in combination with distinct kinds of RNAs are also different. Emerging studies have shown that YTHDC2 is involved in the occurrence and development of lung, gastric, ovarian and other carcinomas through binding to mbA modifications. Binding can then yield a pro- or anti-cancer effect, which is significantly associated with the prognosis of various tumors. This paper summarizes the role and specific mechanisms of YTHDC2 in tumors to provide a reference basis for exploring the prevention, diagnosis and treatment of different tumors from the perspective of YTHDC2. [ABSTRACT FROM AUTHOR]

To meet the needs of flight teaching and training, aiming at the interactive, multi-input multi-output and comprehensive sequential logic controlling characteristic of integrated avionics system display and control interface for fighters, a functional simulation method by designing display and control logic independently is introduced. On the basis of this structure, a loosely coupled, modular and visual simulation method of display and control interface for multi-function fire control radar is presented, and the realization of display and control logic based on Simulink/Stateflow is introduced. The application result shows that the program progress is more intuitive, and the code is more maintainable, so this design method can improve the fidelity and developing efficiency of display and control simulation. All these researches can provide technology reference for the simulation of display and control interfaces for avionics systems and its subsystems. [ABSTRACT FROM AUTHOR]

Objective To investigate the change in mucosal-associated invariant T（MAIT） lymphocytes in peripheral blood of children with metabolic associated fatty liver disease（MAFLD） and its clinical significance. Methods A total of 18 children with MAFLD who attended Hunan Children’s Hospital from March to May, 2022, were enrolled as MAFLD group, and 20 normal children who attended the hospital during the same period of time were enrolled as control group. Peripheral blood samples were collected, and flow cytometry was used to measure the percentages of MAIT lymphocytes（CD3+CDl61+TCRVα7.2+ cells）, different MAIT lymphocyte subsets（CD4+CD8- MAIT cells, CD4-CD8- MAIT lymphocytes, CD4-CD8+ MAIT lymphocytes, and CD4+CD8+ MAIT lymphocytes）, and MAIT lymphocytes expressing PD-1, CD69, perforin, CD107α, CXCR3, CXCR6, and CCR6. The correlation of MAIT lymphocyte frequency with liver inflammation, fat content, and fibrosis degree was analyzed. The t-test was used for comparison of normally distributed continuous data between two groups, and the Mann-Whitney U test was used for comparison of non-normally distributed continuous data between two groups. The Spearman correlation analysis was used for correlation analysis. Results Compared with the control group, the MAFLD group had significant increases in the percentage of MAIT lymphocytes in peripheral blood, the percentages of MAIT cells expressing PD-1, CD69, CD107α, CXCR3, CXCR6 and CCR6, and the percentages of CD4+CD8- MAIT cells and CD4+CD8+ MAIT lymphocytes among MAIT cell subsets（all P<0.05）, as well as a significant reduction in the percentage of CD4-CD8+ MAIT cells among MAIT cell subsets（P<0.001）. The percentages of CD4+CD8+ MAIT lymphocytes and CD107α-positive MAIT lymphocytes were negatively correlated with alanine aminotransferase（r=-0.474 and-0.550, P=0.047 and 0.018）. Conclusion The migration of peripheral blood MAIT lymphocytes to the liver plays a protective role against liver inflammation in children with MAFLD. [ABSTRACT FROM AUTHOR]

BACKGROUND: Walking is the most basic movement mode of the human, exploring the stress characteristics of the foot and the difference between the feet during walking is of great value to deeply understand the law of human walking. OBJECTIVE: To explore the foot function and walking law of human body in each stage of gait support through the processing and analysis of foot balance curve. METHODS: 116 subjects with normal foot type were selected by foot type and walked at their own optimal speed to record their plantar pressure data. A foot balance curve was drawn with average value and 1 times standard deviation. The curve rules and eigenvalues of dominant and non-dominant lateral feet were analyzed. RESULTS AND CONCLUSION: (1) During the walking support period, the force characteristics of the foot on the horizontal side constantly changed. From initial contact phase to forefoot contact phase, the foot stabilized its posture through the force migration. (2) In foot flat phase, the plantar force shifted to the outside. In forefoot push off phase, the plantar force shifted from outside to inside and the human body balanced through bipedal support. (3) There was no significant difference in the relative duration of initial contact phase, forefoot contact phase, foot flat phase, and forefoot push off phase between the dominant foot and the non-dominant foot (P > 0.05). (4) The characteristic similarity of the bilateral foot balance curve was highly consistent and the foot balance curve of the dominant side was lower than that of the non-dominant side (P < 0.05). (5) It is concluded that the changes of foot posture and foot function during human walking are reflected by the foot balance curve. The function of human feet is the same at all stages of the gait cycle, and the force acting on the dominant side is more lateral than that of the non-dominant side, and the pedal and extension function is more fully performed on the horizontal axis. The foot balance curve has an important reference value for the study of human walking law and the evaluation of foot function. [ABSTRACT FROM AUTHOR]

BACKGROUND: Shenling Baizhu San is one of the representative prescriptions for strengthening the spleen and removing dampness, which has good antiinflammatory and immunomodulatory effects. In recent years, we have found positive effect of Shenling Baizhu San in patients with early and mid-stage knee osteoarthritis in clinical practice, but the specific molecular mechanism is still unclear. OBJECTIVE: To explore the pharmacological mechanism of Shenling Baizhu San in the treatment of knee osteoarthritis through network pharmacology and molecular docking. METHODS: The TCMSP and TCMID databases were used to collect the active ingredients and their targets of Shenling Baizhu San. The GenneCards and OMINNCBI databases were used to obtain the KOA targets. STRING and Cytoscape were used to construct the drug-active chemical ingredient-core target network and the protein-protein interaction (PPI) analysis. Gene oncology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were carried out. AutoDockTools was used to predict molecular docking between important active ingredients and core targets. RESULTS AND CONCLUSION: A total of 109 active pharmaceutical chemical components of Shenling Baizhu San were screened, and the important components included luteolin, kaempferol, β-sitosterol, etc. Among them, there were 97 intersecting targets with diseases, and the core targets were PTGS2, CASP3, AKT1, MAPK1, MAPK8, TP53, EGFR, etc. The results of molecular docking showed that kaempferol and β-sitosterol could target and regulate CASP3, AKT1, and PTGS2. GO analysis showed that the mechanism mainly involved biological processes such as oxidative stress, lipopolysaccharide, nutritional level, muscle cell proliferation, oxygen level, etc. The results of KEGG analysis showed that PI3K-Akt, tumor necrosis factor, interleukin-17 and Th17 cell pathways were the main signaling pathways. In summary, Luteolin, kaempferol, β-sitosterol, and other active ingredients in Shenling Baizhu San can treat knee osteoarthritis by inhibiting inflammation, regulating apoptosis, and reducing oxidative stress. [ABSTRACT FROM AUTHOR]

BACKGROUND: Studies have found that icariin can promote the proliferation and differentiation of mouse preosteoblasts. Nuclear factor-κB signaling pathways and autophagy play an important role in the regulation of osteoblast differentiation. Whether they are involved in icariin promoting the proliferation and differentiation of mouse preosteoblasts remains unclear. OBJECTIVE: To investigate the effect of icariin on the proliferation and differentiation of mouse preosteoblasts and the role of nuclear factor-κB signaling and autophagy. METHODS: (1) Screening out the best intervention concentration of icariin: Mouse preosteoblasts MC3T3-E1 cells were divided into control group and various icariin groups (0.01, 0.1, 1, 10, 100 μmol/L). MTS was used to detect the proliferation activity of mouse preosteoblasts from different groups after icariin intervention. Osteogenic differentiation was assessed by Alizarin red staining. The osteoblastic differentiation ability and autophagy activity in mouse preosteoblasts from different groups were evaluated by western blot assay after icariin intervention. (2) 3-Methyladenine was added to inhibit autophagy. Four groups were set up: control group, 3-methyladenine group, icariin group, and 3-methyladenine + icariin group. Alizarin red staining was used to observe the mineralized nodules. Western blot assay was utilized to determine the expression levels of alkaline phosphatase, Runx2, p62, LC3, NF-κB p65 and p-NF-κB p65 protein. RESULTS AND CONCLUSION: (1) Icariin could promote the proliferation of mouse preosteoblasts and the effect was the most obvious at 10 μmol/L (P < 0.05). The formation of mineralized nodules in the 10 μmol/L icariin group was significantly more than that in the control group and the expression levels of alkaline phosphatase and Runx2 protein were significantly increased (P < 0.05). Compared with the control group, the expression levels of Beclin1, LC3 II/LC3 I increased significantly and the expression of p62 decreased in the 10 μmol/L icariin group (P < 0.05). (2) After 3-methyladenine inhibited autophagy, compared with the icariin group, the expression of alkaline phosphatase, Runx2, LC3 II/LC3 I decreased and the expression of p62 increased in the 3-methyladenine + icariin group (P < 0.05). (3) Compared with the control group, the expression of p-p65/p65 decreased in the 10 μmol/L icariin group (P < 0.05). (4) These results indicate that icariin may promote osteogenic differentiation of MC3T3-E1 cells by down-regulation of the nuclear factor-κB signaling and enhancing autophagy. [ABSTRACT FROM AUTHOR]

BACKGROUND: There are few reports about platelet-derived growth factor BB promoting angiogenesis of bone marrow mesenchymal stem cells. However, the nutrition, growth factors and cytokines provided by neovascular network play an important role in bone tissue formation. OBJECTIVE: To investigate the effects of platelet-derived growth factor-BB on the differentiation of bone marrow mesenchymal stem cells of Sprague-Dawley rats into vascular endothelial cells. METHODS: (1) Bone marrow mesenchymal stem cells of Sprague-Dawley rats were isolated and cultured in vitro. The surface markers CD45, CD29 and CD34 of the third generation were identified by flow cytometry. Bone marrow mesenchymal stem cells with high purity were selected for platelet-derived growth factor BB intervention experiments. (2) Passage 3 bone marrow mesenchymal stem cells were induced by 25 μg/L platelet-derived growth factor BB for 3 days. Matrigel was selected for cell lumen formation experiments. The immunofluorescence method was used to detect the expression of FITC-UEA-1 and DIL-ac-LDL in cells to verify the fluorescence expression of CD31 in cells. Flow cytometry was used to detect the expression of CD31 in cells after induction. Finally, the mRNA level of vascular endothelial growth factor gene was detected by RT-PCR. RESULTS AND CONCLUSION: The cells were mixed with Matrigel and inoculated after platelet-derived growth factor BB induction. A clear tubular structure was formed; the expressions of FITC-UEA-1 and DIL-ac-LDL and CD31 expression were positive in bone marrow mesenchymal stem cells after induction. The expression of VEGF mRNA in platelet-derived growth factor BB-induced cells was higher than that in bone marrow mesenchymal stem cells without platelet-derived growth factor BB (P < 0.05). It is concluded that rat bone marrow mesenchymal stem cells can differentiate into functional vascular endothelial cells after being induced by platelet-derived growth factor BB. [ABSTRACT FROM AUTHOR]

BACKGROUND: Icariin is an effective active component of traditional Chinese medicine which is used to prevent osteoporosis, but the specific mechanism remains unclear. Existing studies have indicated that autophagy can participate in regulating bone metabolism; however, whether icariin can prevent osteoporosis by changing autophagy is unknown. OBJECTIVE: To investigate whether icariin can prevent osteoporosis by regulating autophagy and promoting osteoblast differentiation. METHODS: Mouse preosteoblast cell lines MC3T3-E1 in logarithmic phase were selected and treated by different concentrations of icariin (10-6 to 10-3 mmol/L). Cell viability, apoptosis rate, alkaline phosphatase activity, the number of mineralized nodules and the expression level of osteogenic differentiation markers were observed in order to determine the optimal concentration of icariin. Then cells were treated by the optimal concentration of icariin and divided into three groups: control group, icariin group, icariin+3-methyladenine group. The number of autophagosomes, the expression level of osteogenic differentiation markers and autophagy related genes, the expression level of autophagy related proteins were evaluated by MDC staining, RTqPCR and western blot assay, respectively. In an in vivo test, an ovariectomized rat model was established and all the rats were divided into four groups: sham group, ovariectomized group, icariin group, estradiol group. Micro-CT was used to detect the bone micro parameters, and western blot was used to detect the expression level of autophagy related proteins. RESULTS AND CONCLUSION: Icariin could promote the proliferation and differentiation of osteoblasts, and at the same time inhibit apoptosis of the cells. The maximum stimulatory effect of icariin was observed at the concentration of 10-5 mmol/L (P < 0.05). In addition, icariin could increase the number of autophagosomes and upregulate the expression of osteogenic differentiation markers and autophagy-related genes or proteins (P < 0.05), which were antagonized by 3-methyladenine (P < 0.05). Icariin could also improve the parameters of bone microstructure in ovariectomized rats and upregulate the expression of autophagy-related proteins (P < 0.05). All these findings indicate that icariin can prevent osteoporosis by activating autophagy and promoting osteoblast differentiation. [ABSTRACT FROM AUTHOR]

BACKGROUND: It has been reported that platelet-derived growth factor BB secreted by mouse osteoclasts can promote the formation of osteoblasts and vascular cells, but the optimal concentration and time of platelet-derived growth factor BB on bone marrow mesenchymal stem cells are not clear. OBJECTIVE: To investigate the optimal concentration and intervention time of platelet-derived growth factor BB to promote the proliferation of bone marrow mesenchymal stem cells in Sprague-Dawley rats. METHODS: Sprague-Dawley rat bone marrow mesenchymal stem cells were isolated and cultured in vitro. The third generation of cells was identified by surface cytometry CD45, CD29 and CD34, and bone marrow mesenchymal stem cells with higher purity were selected for intervention experiments. The selected cells were seeded in 96-well culture plates. Cells in the control group were cultured with α-MEM complete medium. Cells in the remaining groups were cultured with α-MEM complete medium combined with 6.25, 12.5, 25, 50, and 100 μg/L platelet-derived growth factor BB. CCK-8 method was used to detect the proliferation of cells in each group at 1, 3, 5, and 7 days, and the optimal time and concentration of platelet-derived growth factor BB for bone marrow mesenchymal stem cells intervention. RESULTS AND CONCLUSION: (1) Flow cytometry: mesenchymal stem cells were positive for CD29 (94.6%), negative for CD45 (3.3%), and negative for CD34 (0.2%). Rat bone marrow mesenchymal stem cells with higher purity were obtained. (2) Repeated measurement analysis of variance showed that the absorbance of platelet-derived growth factor BB changed at different times after intervention with bone marrow mesenchymal stem cells. The proliferation effect was the strongest on the third day, and the difference was statistically significant (F=27.773, P < 0.001). LSD method for a pairwise comparison of the grouping factors showed that 25 μg/L platelet-derived growth factor BB group had statistically significant differences with the other five groups (P < 0.05). (3) It was concluded that the 25 μg/L platelet-derived growth factor BB group intervened bone marrow mesenchymal stem cells had a proliferation effect on cells on the third day. Platelet-derived growth factor BB concentration greater than 50 μg/L can promote apoptosis. [ABSTRACT FROM AUTHOR]

[Objective] This paper analyzes the relationship between the ecological types of the peculiar fish in the Qinghai-Tibet Plateau and the evolution of the paleoenvironment. [Method] Using X-ray electron probe micro-area technology (EPMA), the Sr and Ca elements of the plateau loach and small head plateau loach collected from the Tongtian River in the source area of the Yangtze River were analyzed, and the inductance was used at the same time. The coupled plasma mass spectrometer (ICP-MS) measured the concentration of Sr in the water. [Results] It was found that both the plateau loach and the small head plateau loach can be divided into two life history types: (1) The Sr/Ca ratio from the core to the extreme edge of the otolith remains at a low level (<3), The distribution analysis chart is blue with low Sr, showing that the entire life history of the individual is in the water in a low Sr environment; (2) The Sr/Ca ratio fluctuates significantly between 2-15, and the Sr element intensity analysis chart shows a high Sr Green (Study's Plateau Loach) or even red (Small-head Plateau Loach), suggesting its history of high Sr environment. [Conclusion] The Sr element characteristics of the two plateau loach otoliths are consistent with the environmental background values of high Sr in the upper waters of the Tongtian River and relatively low Sr in the lower waters. This research provides a new perspective to understand the changes in the ancient environment of the Qinghai-Tibet Plateau. [ABSTRACT FROM AUTHOR]

BACKGROUND: It is an effective strategy for the treatment of osteoporosis to promote the proliferation and differentiation of osteoblasts, but the influence of inokosterone onosteoblast proliferation and differentiation is rarely reported.OBJECTIVE: To investigate the effects of inokosterone on theproliferation and differentiation of primary osteoblasts and the underlying molecular mechanism.METHODS:Primary osteoblasts from neonatal Sprague-Dawley rats were isolated via the second enzyme digestion, and the cells were then cultured in osteogenic induction medium and identified. Cell counting kit 8 assay was performed to evaluate the effect of different concentrations of inokosterone (1, 5, 10 mg/L) on cell viability of primary osteoblasts. Early differentiation ability of osteoblasts was evaluated by alkaline phosphatase staining and alkaline phosphatase activity assay. To evaluate the mineralization ability of osteoblasts, alizarin red staining was performed to observe the number of calcium nodules. The expression level of osteogenic genes was detected by RT-qPCR at different time points. Furthermore, MDC staining was also used to observe the number of autophagosomes. RESULTS AND CONCLUSION: Compared with the control group, inokosterone could inhibit the cell viability of primary osteoblast to some degree (P < 0.05) while significantly promoting alkaline phosphatase activity and mineralized nodules formation (P< 0.05). In addition, inokosterone upregulated the expression of osteogenic genessuch as Collagen I, osteoprotegerin, osteopontin, osteocalcin and increased the number of autophagosomes. To conclude, inokosterone can promote osteogenic differentiation by upregulating osteogenic genes expression and activating autophagy of primary osteoblasts. [ABSTRACT FROM AUTHOR]

肝细胞癌 (HCC)是肝脏最常见的原发性肿瘤， 目前治疗手段包括手术切除、射频消融、经动脉化疗栓塞 术、免疫治疗等。 程序性死亡蛋白－1 (PD-1)是一种抑制性免疫检查点分子， PD-1 及其配体( PD-Ll) 在 HCC 肿瘤免 疫过程中诱导形成免疫抑制微环境，促进肿瘤增殖和转移。 研究发现在肝癌患者接受手术切除、免疫治疗或其他 常规治疗后， PD-1/PD-Ll 高表达均与患者生存率有关，但 PD-1/PD-Ll 作为临床预后标志物有其局限性。 [ABSTRACT FROM AUTHOR]

Objective: To investigate the clinical manifestations, pathological features and prognostic factors of patients with adrenal lymphoma. Methods: A retrospective analysis was made with the clinical and pathological data of 77 patients with adrenal non-Hodgkins lymphoma confirmedpathology or confirmedimaging evidence in the Department of Hematology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine January 2002 to June 2018. The clinical characteristics of primary adrenal lymphoma (PAL) and secondary adrenal lymphoma (SAL) were compared. Kaplan-Meier curve was used for survival analysis, and Cox regression model was used to analyze the prognostic factors. Results: Of the 77 patients, 28 were PAL and 49 were SAL. The predominant pathological type was difflarge B-cell lymphoma, accounting for 77.9%. There were 21 patients accompaniedbone and gastrointestinal involvement, respectively. The proportions of bilateral involvement (67.9%) and large mass (67.9%) were higher in the PAL patients, while the proportions of Ⅲ / Ⅳ stage (95.9%) and pancreas (24.5%) and bone (38.8%) involvement were higher in the SAL patients. The patients with T/NK cell-derived lymphoma or with incomplete remission after treatment had significantly shorter survival time than those with B cell-derived lymphoma or with complete remission after treatment (P0.005, P0.000). Conclusion: The clinical manifestations of adrenal lymphoma lack specificity. The main pathological type is difflarge B-cell lymphoma. Bilateral involvement and large mass are more common in PAL, while advanced disease and pancreas and bone involvement are more common in SAL. T/NK cell-derived lymphoma and incomplete remission after treatment are major adverse prognostic factors. [ABSTRACT FROM AUTHOR]

In recent years, molecular signaling pathways related to bone metabolism has become a hot topic. Studies have revealed that signaling pathways associated with osteoporosis include Wnt/β-catenin, RANKL/RANK/OPG, NF-κB, PPAR-γ, PTH, MAPK, PI3K/Akt, Hedgehog and Notch signaling pathways. However, there has been no study concentrated on ERα-AMPK-Sirt1 signaling pathway. Estrogen receptor alpha (ERα), AMP-activated protein kinase (AMPK) and Sirtuin 1(Sirt1) can regulate the function of osteoblast or osteoclast by ligand binding, phosphorylation and deacetylation, respectively. Studies have indicated that ERα can directly increase the activity of LKB1, while LKB1 is the most important upstream protein kinase of AMPK, as a result, AMPK is activated. In addition, the mutual promotion of AMPK and Sirt1 can also modulate the autophagy or apoptosis of osteoblast; all these suggested that ERα-AMPK-Sirt1 signaling pathway may play an important role in the pathogenesis of osteoporosis. To sum up, it is necessary to start the research of ERα-AMPK-Sirt1 signaling pathway in order to better guide the development of new drugs and clinical practice. [ABSTRACT FROM AUTHOR]

The specific rate of breakage(S○1)and primary breakage distribution(Bi,j)of untreated and microwave-treated vanadium titano-magnetite(VTM) with different particle size were investigated using grinding kinetics， and the variations of surface topography and phase composition of ground products were analyzed. The results show that the breakages of both untreated and microwave-treated VTM obey the first-order grinding kinetics. The S○1 of microwave-treated VTM is higher than that of untreated ore and the S1 increment increases with increasing particle size. The primary breakage distribution parameters(γ) are dependent upon the feed particle size. The γ value of Bi,j of microwave-treated VTM is less than that of untreated ore. The SEM analysis shows that the ground products have smaller particle size and rougher surface after microwave treatment. The XRD analysis shows that microwave-treated VTM has stronger diffraction peaks and larger amounts of gangue phases after ball mill， compared to those of untreated ore， which indicates that the degree of dissociation of ore is improved. [ABSTRACT FROM AUTHOR]

The cavity perturbation method was used to measure the dielectric properties of ludwigite of different particle sizes at the frequency of 2.45GHz with the temperature ranging from 20 to 800℃， and heating characteristics under a microwave field were also measured.The results showed that with the decrease of particle size， the filling layer porosity of the ore sample decreases， while the dielectric properties and the heating rate increase.When the temperature is higher than 200℃， the thermal decomposition can generate a large number of micro-voids which increases the porosity， and the dielectric properties tend to be decreased， resulting in a reduce of heating rate during microwave heating.The effect of particle size on the dielectric properties and temperature rising characteristics of ludwigite provides a theoretical basis for the application of microwave in the fields of metallurgy and saving energy consumption. [ABSTRACT FROM AUTHOR]

ObjectiveTo investigate the changes in peripheral blood mononuclear cells ［CD8+ T lymphocytes and natural killer (NK) cells］ and levels of perforin and granzyme B in these cells in children with infectious mononucleosis (IM), as well as their clinical significance in liver injury. MethodsA total of 60 children who met the diagnostic criteria for IM were enrolled, and 30 healthy children were enrolled as control group. With the help of cell surface markers and cytokine staining, flow cytometry was performed to analyze CD8+ T lymphocytes, NK cells, and the expression of perforin and granzyme B. The t-test was used for comparison of continuous data between groups; a one-way analysis of variance was used for comparison between three groups, and the Dunnett-t test was used for further comparison between two groups. ResultsAmong the 60 children with IM, the incidence rate of liver injury was 50% (30/60); 18 (60%) children had an alanine aminotransferase (ALT) level of ＜200 IU/L, 10 (33.3%) had an ALT level of ≥200 IU/L and ＜400 IU/L, and 2 (6.67%) had an ALT level of ≥400 IU/L. All children had normal liver functions after one month of treatment. Compared with the control group, the non-liver injury IM group and the liver injury IM group had significant increases in the expression of perforin and granzyme B in CD8+ T lymphocytes (all P＜0.05). Compared with the non-liver injury subgroup of IM patients, the liver injury subgroup had a significantly higher percentage of CD8+ T lymphocytes and significantly higher expression of perforin and granzyme B in NK cells (all P＜0.05). ConclusionThere is a high incidence rate of liver injury in children with IM, mainly mild or moderate elevation of aminotransferases, which are self-limiting and can be returned to normal. High levels of perforin and granzyme B in NK cells and a high percentage of CD8+ T lymphocytes are the cause of liver injury. [ABSTRACT FROM AUTHOR]

Objective To evaluate the clinical characteristics and preoperative predictive factors in patients with parathyroid carcinoma. Methods From January 2010 to December 2016, 150 patients with pathologically and biochemically confirmed primary hyperparathyroidism were enrolled in this study. The clinical data were reviewed to define 28 cases of parathyroid hyperplasia, 14 cases of parathyroid carcinoma (PC), and 108 cases of parathyroid adenoma (PA). The clinical characteristics between PC and PA group were compared and analyzed to explore predictive factors for PC. Results Patients in PC group were significantly younger than those in PA group (47.9±13.9 vs 57.2±14.6 years, P=0.026). The diameter of tumor in PC group was larger than that in PA group (3.6±1.8 vs 2.2±1.4 cm, P =0.005). The levels of albumin-corrected serum calcium, parathyroid hormone (PTH), and alkaline phosphatase (AKP) were all higher in PC group than those in PA group (3.4±0.6 vs 2.9±0.3 mmol/l; 1 157.1 vs 201.6 pg/ml; 168 vs 97 IU/L； all P<0.05). PC correlated with age( r = -0.241, P = 0.007) , albumin-corrected serum calcium (r = 0.324, P<0.001) , PTH levels ( r = 0.301, P = 0.001) , serum AKP ( r = 0.217, P =0.019) and tumor size( r = 0.238, P = 0.011). Receiver operator characteristic curve analysis showed that the optimum cut-off values as follows ： albumin-corrected serum calcium 3.15 mmoL/L, serum PTH concentration 1 011 pg/ml and tumor diameter 3. 35 cm. These indicators can be helpful in the preoperative diagnosis of PC. Conclusions Patients with PC were younger than the patients with PA. Albumin-corrected serum calcium, serum PTH concentration, and tumor diameter can be helpful in preoperative diagnosis of PC. [ABSTRACT FROM AUTHOR]

Ｉｎ ｏｒｄｅｒ ｔｏ ｓｏｌｖｅ ｔｈｅｃｏｏｒｄｉｎａｔｅ ｔｒａｎｓｆｏｒｍａｔｉｏｎ ａｎｄ ｃｏｍｐｌｅｘ ｃａｌｃｕｌａｔｉｏｎｏｆ ｔｒａｄｉｔｉｏｎａｌ ＳＶＰＷＭ ａｌｇｏｒｉｔｈｍꎬ ｔｈｅ ｍｅｃｈａｎｉｓｍ ｏｆ ＳＶＰＷＭ ａｌｇｏｒｉｔｈｍ ｗａｓ ｄｅｄｕｃｅｄ ａｎｄ ａｎａｌｙｚｅｄꎻ ｔｈｅ ｉｎｈｅｒｅｎｔ ｌａｗ ｏｆ ｔｈｒｅｅ￣ｐｈａｓｅ ｓｐａｃｅ ｖｅｃｔｏｒ ｍｏｄｕｌａｔｉｏｎ ｗａｓ ｓｔｕｄｉｅｄ ａｎｄ ａ ｍｏｄｉｆｉｅｄ ｎｏｎ￣ｓｅｃｔｏｒ ａｌｇｏｒｉｔｈｍ ｂａｓｅｄ ｏｎ ａｂｃ ｃｏｏｒｄｉｎａｔｅｓ ｗａｓ ｐｒｏｐｏｓｅｄ. Ｔｈｅ ｐｒｏｐｏｓｅｄ ｍｏｄｕｌａｔｉｏｎ ａｌｇｏｒｉｔｈｍ ｗａｓ ｕｓｅｄ ｔｏ ｆｕｌｆｉｌｌ ｔｈｅ ｔｒａｄｉｔｉｏｎａｌ ＳＶＰＷＭ ｆｕｎｃｔｉｏｎ. Ｂｙ ｕｓｉｎｇ ｔｈｅ ｉｎｔｅｒｎａｌｒｅｌａｔｉｏｎｓｈｉｐ ｏｆ ｔｈｅ ｔｈｒｅｅ￣ｐｈａｓｅ ｖｏｌｔａｇｅꎬ ｔｈｅ ｍｏｄｕｌａｔｉｏｎ ｔｉｍｅ ｆｕｎｃｔｉｏｎ ｏｆ ＳＶＰＷＭ ａｌｇｏｒｉｔｈｍ ｗａｓ ｄｅｄｕｃｅｄꎬ ａｎｄ ｔｈｅ ｃｏｍｐｕｔａｔｉｏｎａｌ ｃｏｍｐｌｅｘｉｔｙ ｏｆ ＳＶＰＷＭ ａｌｇｏｒｉｔｈｍ ｗａｓ ｒｅｄｕｃｅｄ ｗｉｔｈｏｕｔ ｃｏｍｐｌｅｘ ｃｏｏｒｄｉｎａｔｅ ｔｒａｎｓｆｏｒｍａｔｉｏｎ ａｎｄ ｓｅｃｔｏｒ ｊｕｄｇｍｅｎｔ. Ｔｈｅ ｍｏｄｉｆｉｅｄ ＳＶＰＷＭ ａｌｇｏｒｉｔｈｍ ｗａｓ ｔｈｅｎ ａｐｐｌｉｅｄ ｔｏ ｔｈｅ ２４５ ｋＶＡ ｓｕｂｗａｙ ａｕｘｉｌｉａｒｙ ｉｎｖｅｒｔｅｒ ｔｏ ｒｅａｌｉｚｅ ｔｈｅ ａｃｃｕｒａｔｅ ｃｌｏｓｅｄ￣ｌｏｏｐ ｃｏｎｔｒｏｌ ｏｆ ｔｈｅ ｔｈｒｅｅ￣ｐｈａｓｅ ｖｏｌｔａｇｅ. Ｔｈｅ ｔｈｅｏｒｙ ａｎｄ ｅｘｐｅｒｉｍｅｎｔ ｓｈｏｗ ｔｈａｔ ｔｈｅ ａｌｇｏｒｉｔｈｍ ｄｏｅｓ ｎｏｔ ｒｅｑｕｉｒｅ ｃｏｍｐｌｅｘ ｃａｌｃｕｌａｔｉｏｎꎬ ａｎｄ ｈａｓ ｔｈｅ ａｄｖａｎｔａｇｅｓ ｏｆ ｑｕｉｃｋｎｅｓｓ ａｎｄ ｓｉｍｐｌｉｃｉｔｙꎬ ａｓ ｗｅｌｌ ａｓ ｐｒａｃｔｉｃａｌｉｔｙ ａｎｄ ｆｅａｓｉｂｉｌｉｔｙ ｉｎ ｔｈｅ ｔｈｒｅｅ￣ｐｈａｓｅ ｉｎｖｅｒｔｅｒ. [ABSTRACT FROM AUTHOR]

According to the characteristics of the complex hard-processing vanadium titano-magnetite (VTM) resources in China， the influence of microwave irradiation on the grinding of VTM was investigated and the features of selective crushing and boundary cracking of VTM under microwave treatment were revealed. The results showed that the ore temperature increased with the increase of the particle size of VTM and the temperature rising capability of VTM was mostly influenced by microwave power. XRD and SEM analyses indicated that after being pretreated by microwave irradiation， a number of intergranular cracks appeared and more monomer was dissociated in VTM.The mass fraction of particle-size grade less than 0.074mm in the ground product increased from 72% to 95% after being radiated by 4kW microwave for 100s. [ABSTRACT FROM AUTHOR]

To meet the demand for simulating the 3D landform of flight simulator and other 3D holographic projection simulators， a curved surface interpolation subdivision rule was constructed and applied. The ideal terrain could be generated by adjusting the parameters of the interpolation polynomial. The rule is proved to be an easy，ast and effective way of landform simulation. [ABSTRACT FROM AUTHOR]

Experimental study on the steel casting with pin connection was carried out. Castings with three different thicknesses or 100,150 mm and 182 mm were tested and the maximum tensile force was 8 000 kN. Results show that there are plastic deformations in different degrees on castings and the area of plastic zone which is roughly shear type decreases significantly with the increase of specimens' thickness. Due to stress concentration the area where casting and pin contact directly yields quickly. The contact deformation results in stress redistribution. Then the strains of some gauges decrease and tension and compression strains are even reversed. Comparison with FEA results shows that there is a great stress concentration where casting and pin contact in early stage of loading when steel casting is under eccentric force. Results of design formulas of different countries' standards are compared with the test results and the recommended value of the strength amplification factor is 1.2. [ABSTRACT FROM AUTHOR]

Objective To evaluate the scientific quality of the patient-reported outcome instrument for primary osteoporosis. Methods We retested 52 primary osteoporosis patients and 92 healthy volunteers to evaluate the difficulty, discrimination, reliability, validity and feasibility of the OPPRO instrument by using the classical test theory and modern item response theory methods. Results The IRT analysis showed that the difficulty and discrimination of the OPPRO instrument are within acceptable parameters. For reliability, the Cronbach's coefficient of the whole instrument is 0. 801, the coefficient of the main symptoms aspect of the instrument is 0. 907, the systemic performance aspect 0. 780, the psychological aspect 0. 849, the social aspect 0. 807, the performance in daily activities aspect 0. 799,and the performance satisfaction aspect 0. 877. All of these results represented a good internal consistency of the instrument. The Pearson correlation coefficient of the retest reliability of the instrument is 0. 925, the Kendall correlation coefficient is 0. 759, both of them suggests a good retest reliability. For validity, the structural validity of the results indicates that the multidimensional structure of the instrument meets to our professional expectations. For the feasibility analysis,the sampling survey of the primary osteoporosis patients showed that the total completion rate of the instrument is 90. 34% and the time required to complete is about 15 minutes. Conclusion By using the classical statistical testing and the item response theory to analyze the items of the patient-reported outcomes of primary osteoporosis, we found that the OPPR instrument has good difficulty, discrimination, reliability, validity and feasibility. The study was conducted strictly in standard steps and took consideration of Chinese culture. The scope of the research, the methodology and the reporting methods are of a high quality, which represents good innovation, scientific quality and as well as clinical significance. The OPPRO is a useful tool for evaluating the clinical outcomes in patients with primary osteoporosis. [ABSTRACT FROM AUTHOR]

Aiming at characterizing the load of earthmoving machinery under non-stationary random cyclic conditions for design and tests of its components， a parameterized model was proposed. In order to study the multi-way valve in the hydraulic excavator， the outlet pressure at valve port was decomposed into random term and trend term by wavelet transform. The stationary random term was described by “random term function” using the estimation of power spectral density， and the non-stationary trend term was expressed by “trend term function” using the function to fit with condition-variables. Then the two functions were combined to generate “cyclic condition load function”. Comparisons between simulated and test data prove that this function can accurately reconstruct random character in multi-way valve under non-stationary random cyclic conditions. The results also indicate that the modeling method will be an applicative reference for realizing the parametric expression of non-stationary random load with cyclic features in engineering application. [ABSTRACT FROM AUTHOR]

Water supply and drainage pipe networld system plays an important role in undergraduate teaching of water supply and drainage science and engineering. Due to the lack of experimental teaching equipment, there is only theoretical teaching in the course of water supply and drainage pipe network system,instead of relevant experimental teaching. In order to solve this problem, a simulation system of water logging in drainage network was independently developed on the basis of environmental online-monitoring technique. The network would be applied to undergraduate experimental teaching. This experimental equipment consists of two parts： the model of drainage network and on-line monitoring system. According to this experimental equipment, students can learn better the composition and operation of drainage network. They can also learn how to optimize the operation of drainage network by the analysis of on-line monitoring data. [ABSTRACT FROM AUTHOR]

Objective: To explore the expression and clinical significance of BMP - 7 in preeclampsia kidney damage. Methods: 98 cases of patients with preeclampsia kidney damage from obstetrics and gynecology department from March 2012 to December 2013 were selected as the research object, and another 30 cases of healthy pregnant women was considered as controls. The concentration of serum and urine BMP - 7 protein and beta 2 microglobulin (β2-MG) in urine was detected by ELISA, 24 h urine protein quantitative determination was performed with benzyl cable chloramine turbidimetry. Results: ① Compared with control group, the concentration of urine and serumBMP 7 protein in the preeclampsia group was decreased significantly (P < 0.05); ②With the progression of disease, the concentration of urine and serum BMP 7 protein, the quantity of urine albumin of β2-MG was significantly increased in preeclampsia group (P < 0.05);③the concentration of urine and serumBMP 7 protein in preeclampsia group was respectively positively correlated with quantity of urinary albumin and β2-MG (P < 0.05). Conclusions: The serum BMP 7 protein is significantly reduced in the patients with preeclampsia renal damage, which can be used to evaluate the progress of preeclampsia renal damage, and provide guidance for clinic. [ABSTRACT FROM AUTHOR]

Allogeneic hematopoietic stem cell transplantation has become one of the effective means of treatment of certain malignancies. But with its of graft versus host disease(GVHD) is one of the important complications of death patients after transplantation.Therefore, how to induce GVHD after transplantation immune tolerance to control, especially control the occurrence of acute graft versus host disease(a GVHD) has become an important part of the study. The occurrence of GVHD mechanism is very complicated. But ultimately identify for mature T cells within the bone marrow donor recipient cells on the surface of the MHC- I and MHC II and caused peptides presented by donor T cell activation, proliferation and infiltration of GVHD target organs such as skin, small intestine and the liver and lead to target organs damage. Clinical implantology and transplantation immunology is one of the contents of the main crack how to control the occurrence of GVHD development. The prevention and treatment is the key to success of allogeneic hematopoietic stem cell transplantation. Individual transplantation is one of the main factors of long-term survival. Related cytokines application and related mechanism research, to a great extent, effective control of the development of GVHD. such as CD4+CD25+Tregs, ecto-59-nucleotidase(CD73), natural killer cell(NK cells), Granulocyte colony-stimulating-factor(G-CSF), Mesenchymal stem cells(MSC), Inducible T cell costimnlator(ICOS) and so on.This article about the cause of GVHD phenomenon and summarize the latest progress. [ABSTRACT FROM AUTHOR]

Gastric cancer is one of the common malignant tumors. It has a higher morbidity than other tumors and its death toll accounts for a quarter. There are nearly 400,000 newly increased patients per year, but the early diagnostic rate is lower than 20%. So gastric cancer has become one of the most serious diseases that endanger our healthy. Interleukins, as the interaction lymphatic factors between white blood cells and immune cells, play an important role in mediating the activation, proliferation and differentiation of the T and B cells, as well as the inflammatory response. In recent years, more and more scholars found that, they have an intimate contact with the occurrence and development of tumor. So far, 29 kinds of interleukins have been discovered and have been named IL-1～IL-29, they bear the corresponding mission respectively. A large number of experiments and literature at home and abroad showed that interleukin-1, 6, 8 have a close relationship with the gastric cancer. It puts forward a new idea for the early diagnosis and treatment, and then improve the early diagnostic rate and treatment of gastric cancer. In this paper, the sources, molecular structure and receptor of IL-1, IL-6, IL-8 are summarized briefly, and the biological characteristics and the relation with gastric cancer are also described. [ABSTRACT FROM AUTHOR]

目的：探讨腹腔镜胆囊切除术治疗胆囊结石的临床疗效。方法：选取2020年2月至2022年11月在我院接 受胆囊切除术的患者100例作为研究对象，根据临床治疗情况分为腹腔镜组 (80例)和开腹组 (20例)。开腹组不进 行临床路径管理，腹腔镜组进行临床路径管理。比较两组患者的治疗效果。结果：腹腔镜组总有效率高于开腹组， 差异有统计学意义 (P < 0.05)，与开腹组差异有统计学意义 (P < 0.05)；治疗前社会功能、精神状态和主观症状、 生理状态、生活质量评分和总分无显着差异 (P > 0.05)，治疗后腹腔镜组的生活质量评分和总分均高于腹腔镜组患 者。组与开腹组比较，差异有统计学意义 (P < 0.05)。结论：腹腔镜胆囊切除术治疗胆囊结石的临床路径具有显着 影响，可有效应用于腹腔镜胆囊切除术后胆囊结石患者的治疗，有助于不断提高治疗质量和治疗效果。 [ABSTRACT FROM AUTHOR]

Here the multi-source data fusion of soil water content was proposed to improve the regional applicability and accuracy of grid data using decomposition and reconstruction of the discrete wavelet transform. The products of soil water content were estimated to test the feasibility and applicability of the improved fusion from the ESA-CCI, SMAP, and ERA5-Land. The data was also collected from the ground station in the Yellow River Basin, including the main agroclimatic region from June to September 2016-2018. The results showed that the improved fusion effectively captured the multi-scale feature information from the original data source. These multi-source and multi-scale feature information was then weighted to merge and reconstruct at each decomposition level. Finally, the regional applicability was effectively improved in the different agroclimatic regions, together with the accuracy of spatiotemporal structure and fluctuation. The merged data of soil water content was fully assessed, with the root mean square error (RMSE) of 0.053 m3 /m3, the bias of 0.001 m3 /m3, and the correlation coefficient (r) of 0.721. Furthermore, the merged data of soil water content was also much better than any single original one, in terms of the overall performance of spatiotemporal decomposition. The higher time-series RMSEs of ERA5-Land soil water content were found in the transition region from the humid to the semi-arid agricultural climate zone, as well as the SMAP and ESA-CCI soil water content in the rainy region. By contrast, the spatial-series RMSEs steadily fluctuated with a narrow range interval (0.07-0.08 m³/m³), especially the amplitude of the fluctuation was controlled within 0.1 m³/m³ during increasing precipitation. The merged data of soil water content shared the lower positive bias (0.005 m³/m³) in the time series and lower negative bias (-0.002 m³/m³) in the spatial series, indicating the higher positive bias of ERA5-Land and ESA-CCI soil water content, together with the overall negative bias of SMAP soil water content. There was a similar spatiotemporal range in the correlation coefficient of the merged and ERA5-Land soil water content, indicating a slightly lower in the time series (0.578), and much higher in the spatial series (0.497). The higher matching of the merged data was achieved in the structural characteristics of the multi-scale spatiotemporal fluctuation spectrum in the observation. The higher total power of cross wavelet and stronger ability for the merged data were observed to characterize the spatiotemporal fluctuations of real soil water content. There was consistent spatiotemporal fluctuation within the time scale of 25 d. The fusion scheme was considered in the multi-dimensional trends and structure characteristics from the three data sources at different spatial scales in the fusion processing of multi-level wavelet decomposition and weighted merging level by level, compared with the Triple-Collection Analysis, and Cumulative Distribution Function Matching. Hence, the maximum layer was determined for the wavelet decomposition. There was a slightly lower linear slope of the merged soil water content, compared with the observations, due to the common feature of “overestimation of lower value, and underestimation of high value” of ERA5-Land, ESA-CCI, and SMAP soil water content. Feasible approaches in the future can be expected to improve the fusion data sources and algorithm, such as the spatiotemporal continuity and accuracy of ESA-CCI and SMAP soil water content. The observations were introduced into the fusion processing, and then the advanced fusion was developed to merge the more useful information relating to spatiotemporal characteristics of soil water content. In short, a better expectation was obtained to improve the regional soil water content. The finding can provide feasible and effective applications in the regional ecological environment, sustainable agricultural development, soil and water conservation, as well as disaster prevention and mitigation. [ABSTRACT FROM AUTHOR]

本文报道一例起搏器植入后改善运动性晕厥症状的病例。中年女性，半年内发生活 动后晕厥数次，可自行恢复意识，入院后查体未见明显异常，头颅磁共振成像及冠脉造影均未 见异常，行平板运动试验提示运动后出现窦性心动过缓，窦性停搏，考虑诊断为窦房结变时功 能不良，遂予起搏器植入治疗，随访半年患者再未出现运动后晕厥症状。 [ABSTRACT FROM AUTHOR]

本文报道一例起搏器植入后改善运动性晕厥症状的病例。中年女性，半年内发生活动后晕厥数次，可自行恢复意识，入院后查体未见明显异常，头颅磁共振成像及冠脉造影均未见异常，行平板运动试验提示运动后出现窦性心动过缓，窦性停搏，考虑诊断为窦房结变时功能不良,遂予起搏器植入治疗，随访半年患者再未出现运动后晕厥症状。 [ABSTRACT FROM AUTHOR]

Objective To explore the hepatic steatosis in osteoporosis (OP) model rats and the effect of the nourishing kidney, invigorating spleen, and promoting blood flow recipe. Methods Twenty-four 3-month-old SPF-grade female rats were optionally allocated into the sham-operation group (n=6) and the mock-up group (n=18). After the ovaries were removed to ascertain the success of modelling, 12 rats in the mock-up were randomly sampled, and the rats were gavaged with alendronate (n=6) and the nourishing kidney, invigorating spleen, and promoting blood flow recipe (n=6) for three months. BMD was detected using DXA. Micro CT was used to observe the bone microstructure. Biochemical tests were performed to detect ALT and AST. Oil-red O staining and HE sections were used to observe the changes in the liver. Immunohistochemistry was performed to observe the changes of glutathione peroxidase 4 (GPX4). Results Compared to those in the sham group, ALT and AST in the mock-up group increased (P<0.05). BMD of the lumbar spine in the model group was lower than that in the sham-operated group (P<0.05). The number of bone trabeculae in the mock-up group was reduced and the bone microstructure was damaged. Fat infiltration in the liver was obvious in the mock-up group, and the hepatic lobes were unclear and the hepatic cords were far from regular. After intervention with the nourishing kidney, invigorating spleen, and promoting blood flow recipe, the fat infiltration was reduced and the hepatic lobes were clearer, and the hepatic cords were regular. Comparing to that in the mock-up group, the expression of GPX4 decreased in the model group (P<0.05). Conclusion Osteoporosis may also lead to fatty lesions in the liver. The nourishing kidney, invigorating spleen, and promoting blood flow recipe has the ability to slow down the fatty lesions in the liver of the rats. GPX4 may be involved in the relationship between osteoporosis and the progression of fatty liver. [ABSTRACT FROM AUTHOR]

Using germinating seedlings of Schizonepeta tenuifolia as materials,the optimum concentration and treatment time of colchicine induced chromosomal ploidy variation were carried out,the agronomic characters of polyploid plants were investigated and compared. The results showed that 0. 10% colchicine solution was the most beneficial to tetraploid production at the growth point 3 d of Schizonepeta tenuifolia seedlings, and the induction rate reached 1. 15%. The number of chromosome in the root tip of the mutant plants was 2n = 4x = 24. Compared with diploid plants, tetraploid plants had thicker leaves, darker green leaves, more dense ears and larger seeds, and the content of effective components in tetraploid plants was higher than that in diploid plants. [ABSTRACT FROM AUTHOR]

Structural variation is an important type of genome variation, which affects molecular and cellular processes, regulatory functions, and brings great influence of the regulation of gene expression and individual phenotype. The accurate detection of population-scale structural variation helps to draw the full spectrum of population genome variation, which reveals the characteristics of population genetics and evolution, and gives support for disease analysis and precision medicine. This paper provides a workflow of structural variation detection from population genomes based on high-throughput sequencing data. The workflow achieves comprehensive and accurate structural variation detection through multiple high-performance structural variation detection algorithms. The multilayer integration and filter were applied to achieve set of candidate structural variation with high precision. By performing genotype correction, variation trimming, and type revising, the spectrum of structural variation of population genomes was obtained. In this study, structural variation detection was performed through the workflow on a population group containing 267 individuals, and 96 202 structural variations were reported. The types of variation and distributions of variation frequencies corresponded to those in other international genome projects, which indicates that the workflow has outstanding ability for structural variation detection from population genomes. Meanwhile, the parallel workflow significantly decreases the analysis time while maintaining the memory cost, which gives strong support for large-scale population structural variation detection. [ABSTRACT FROM AUTHOR]

目的 探讨中医辨证治疗联合人工泪液在干眼症的临床治疗中的作用.方法 选取该院2014年9月至2016年9月收治的干眼症患者60例,并采用随机数字表法将其分为对照组和观察组,各30例.对照组使用人工泪液,即右旋糖酐70滴眼液治疗.观察组在对照组的基础上加用中医辨证疗法,根据不同的症型选择不同方剂加减治疗.比较两组患者治疗后的中医症候积分和治疗效果,以及治疗后泪膜破裂时间(BUT)、泪液分泌实验(SIT)、角膜荧光染色(FL)和角膜表面规则性指数(SRI)、角膜表面不对称指数(SAI).结果 观察组患者治疗后中医症候积分较对照组明显下降,且治疗有效率高于对照组,差异均有统计学意义(P<0.05);此外,治疗后观察组患者BUT和SIT较对照组延长,而FL、SRI和SAI则低于对照组,差异均有统计学意义(P<0.05).结论 人工泪液联合中药辨证治疗干眼症疗效较好,值得临床推广应用. [ABSTRACT FROM AUTHOR]

中医养生学是我国传统文化的集大成者,是中医学宝库中的一颗璀璨明珠。随着疾病谱和医 学模式的转变,老龄化社会进程日益加剧,中医养生成为时下养生保健的有效选择。因此,中医养生学的教学 也面临巨大挑战,作者根据该校具体实际情况及中医养生学教学经验,对中医养生学课程的教学方法进行了 改革。 [ABSTRACT FROM AUTHOR]

: In order to explore the copper absorption characteristics of Anodonta woodiana, this study was based on the juveniles with higher Cu absorption efficiency as the experimental subjects. Five concentration gradients (2.0, 1.0, 0.1, 0.01 and 0.005 mg·L-1) of aquatic Cu were set according to its 96 h-EC50 to the mussels and China Fisheries Water Quality Standard (GB11607-89). This exposure experiment was conducted for 24 h. The results showed that the Cu absorption efficiency increased rapidly with the increase of exposure concentration, and the highest value was (0.69±0.11) μg/(g·h) in 2.0 mg·L-1 exposure group. However, the Cu removal rate showed a general decreasing trend, in which the removal rate of 0.005 mg·L-1 exposure group was the highest 84.8%, and the removal rate of 1.0 mg·L-1 exposure group was the lowest 28.9%. In conclusion, the high Cu uptake efficiency of juvenile A. woodiana indicates that it has a very high application potential in the prevention and control of heavy metal Cu pollution in freshwater fishery waters and in the development of model organisms for monitoring and evaluating the environmental Cu pollution in freshwater fishery waters. [ABSTRACT FROM AUTHOR]

In order to efficiently and accurately predict traffic conflicts between motorized and non-motorized vehicles at unsignalized roundabouts, a combined prediction model (SVR-GA-BP) based on genetic algorithm optimized BP neural network (GA-BP) and support vector machine regression (SVR) was proposed. The high-precision mixed traffic flow trajectory data was collected using drone video at an unsignalized roundabout. The video recognition software Tracker was used to extract the trajectory data of motorized and non-motorized vehicles conflicts. The time to collision (TTC) parameter was chosen as the discriminant index to determine the severity of motorized and non-motorized vehicles conflicts. Based on partial correlation analysis, the traffic volume, average speed and percentage of heavy vehicles were determined as the significant influencing factors for conflicts. Five evaluation metrics, such as root mean square error (RMSE) and mean absolute error (MAE), were selected to analyze the accuracy of the predicted values of SVR model, BP neural network and SVR-GA-BP model. The results show that the accuracy of the combined model in minor conflict prediction is 97. 1%, which is 6. 9% and 2. 5% higher than that of SVR and BP model respectively. The accuracy of the combined model in serious conflicts prediction is 96. 1%, which is 7. 3% and 5. 1% higher than that of SVR and BP model respectively. It can be seen that the SVR-GA-BP combined model can effectively predict the motorized and non-motorized vehicles traffic conflict of unsignalized roundabout with the highest accuracy, which can provide reference for the safety evaluation of the same type of intersections. [ABSTRACT FROM AUTHOR]

目的 比较氨磺必利和利培酮对精神分裂症患者血清泌乳素水平的影响。方法将80例成人精神分裂症患者随机分为A、B组各40例,分别采用氨磺必利和利培酮治疗12周,治疗前及治疗后4、12周检测血清泌乳素。结果治疗后12周末,两组血清泌乳素水平均较治疗前升高（P均〈0.05）,且B组女性患者血清泌乳素水平均高于A组（P〈0.05）;泌乳素水平显著升高（高于正常值上限4倍以上）A组16例、B组18例,P〉0.05。结论 氨磺必利对女性精神分裂症患者泌乳素水平影响较利培酮小。 [ABSTRACT FROM AUTHOR]

目的：观察注射用鼠神经生长因子治疗视神经挫伤的效果及安全性。方法将54例（54眼）视神经挫伤患者随机分为观察组30例及对照组24例。两组均给予口服强的松、甲钴胺片及维生素等常规治疗，观察组给予注射用鼠神经生长因子30μg，肌内注射，1次／d，21 d为1个疗程，共注射2个疗程。比较两组治疗前后最佳矫正视力（BCVA）、视野平均缺损（MD），并统计不良事件发生情况。结果两组治疗后BCVA较治疗前不同程度提高，1个疗程后，观察组及对照组总有效率分别为70．00％、41．67％，2个疗程后分别为93．33％、54．17％，两组总有效率比较，P均＜0．05。1个疗程后观察组与对照组MD分别为12．72±5．04、15．73±5．21，两组比较，P＜0．05；2个疗程后观察组与对照组MD分别为10．64±5．86、15．11±5．41，两组比较，P＜0．01。两组治疗过程中均未出现严重不良事件。结论注射用鼠神经生长因子治疗视神经挫伤疗效确切，且较为安全。 [ABSTRACT FROM AUTHOR]

BACKGROUND: Relaxin can significantly improve cardiac and renal dysfuction caused by pathological factors, inhibit myocardial hypertrophy, have an anti-fibrosis effect, and improve ischemia-reperfusion injury. However, its protective mechanism against hypoxia-reoxygenation injury of endothelial cells remains unclear. OBJECTIVE: To investigate the protective mechanism of relaxin against hypoxia-reoxygenation injury of myocardial microvascular endothelial cells. METHODS: Mouse myocardial microvascular endothelial cell line (H5V cells) was used for the experiment. Cells were treated by three different interventions: in control group, cells were normally cultured for 33 hours; in model group, cells were treated by 6-hour hypoxia followed by 3-hour reoxygenation); and in relaxin group, 24 hours of routine culture (180 ng/mL relaxin), 6 hours of hypoxia and 3 hours of reoxygenation (180 ng/mL relaxin) were given to simulate myocardial hypoxia-reperfusion injury. Cell permeability and Caspase-3 activity were then detected. Levels of tumor necrosis factor-α, interleukin-1β and interleukin-6 in cell supernatants were detected by ELISA. Expressions of VE-cadherin, Akt, and GSK-3β at mRNA and protein levels were detected by RT-PCR and western blot, respectively. RESULTS AND CONCLUSION: Compared with the control group, the cell permeability and expression of caspase-3 increased significantly in the model group (P < 0.05). Compared with the model group, the cell permeability and expression of caspase-3 decreased in the relaxin group (P < 0.05). Moreover, the levels of tumor necrosis factor-α, interleukin-1β and interleukin-6 were elevated in the model group, while the levels were significantly decreased after relaxin treatment (all P < 0.05). There were no significantly changes in the mRNA and protein expressions of VE-cadherin, Akt1, and GSK-3β mRNA among three groups (all P > 0.05). Compared with the control group, the expression of phosphorylated VE-cadherin, Akt1 and GSK-3β were decreased in the model group (P < 0.05), and relaxin treatment reversed these changes to the control levels (P < 0.05). To conclude, relaxin treatment could enhance VE-cadherin expression, reduce hypoxiareoxygenation-induced microvascular endothelial cell damage, inhibit inflammatory cytokine release, and reduce cell apoptosis, which may be related to the activation of the Akt/GSK-3β signaling pathway. [ABSTRACT FROM AUTHOR]

In order to improve the ability of transmission towers to resist extreme meteorological disasters in service and ensure the safe and stable operation of transmission lines, a typical 220 kV transmission tower is taken as an example, and the finite element method is used to study its wind resistance ultimate load bearing capacity and analyze the collapse mechanism and damage mode of transmission towers under strong wind. In view of the failure mode of the transmission tower, a non-destructive reinforcement scheme is proposed. By establishing finite element models of angle steel members with different length to slenderness ratios of the main material, the axial compression load capacity of the main material before and after reinforcement is numerically simulated, also the reinforcement design is carried out for the main material of the weak part of the transmission tower, and the ultimate wind bearing capacity of the reinforced transmission tower is studied. The results show that the reinforced main material angle bearing capacity is greatly increased, up to 37.2%. The local stiffness of the weak parts of the transmission tower is improved, the top displacement and the maximum stress of the tower are significantly reduced compared with those before reinforcement, the ultimate bearing capacity of the tower is greatly increased, and the design importance factor of the transmission tower is increased from 1.15 to 1.35. The proposed reinforcement method can provide an important reference for the wind resistance reinforcement design of the same type of transmission tower. [ABSTRACT FROM AUTHOR]

This study was conducted to investigate the purification capacity of the freshwater mussel Anodonta woodiana on nitrite ( NO2- -N) in rearing water. The effect of nitrite on the mussels’ clearance rates was analyzed. Furthermore, the nitrite concentrations, removal efficiencies, and purification efficiencies were compared at the control (0 mussels/ m³ ), low (10 mussels/ m³ ), medium (20 mussels/ m³ ), and high (30 mussels/ m³ ) culture densities and different treatment times (24, 48, 72, and 96 h). The results showed that the clearance rates of A. woodiana in the blank (0 mg / L) and nitrite-treated (1. 0 mg / L) groups were 232. 1 ±122. 3 L / (h·kg) and 69. 0±17. 7 L / (h·kg), respectively, they did not reach a significant difference level (P>0. 05). The lowest nitrite concentration in the rearing water was 0. 20±0. 06 mg / L for A. woodiana at low culture density and 96 h treatment, and the corresponding removal efficiencies and purification efficiencies could reach(64. 99±10. 32)% and 90. 16±13. 54 mg / ( h·kg), respectively. The effect of A. woodiana on nitrite concentrations and removal efficiencies was significantly correlated with culture density and treatment time (P<0. 05), while the interaction with both was not significant(P>0. 05). The purification efficiencies of nitrite was only significantly correlated with culture density ( P < 0. 05 ). These findings showed that A. woodiana could effectively purify the higher levels of nitrite in the rearing water, and the best purification effect was achieved at a culture density of 10 mussels/ m³. [ABSTRACT FROM AUTHOR]

BACKGROUND: Serelaxin is a recombinant form of human relaxin 2. As endogenous anti-fibrosis active substances, serelaxin may relieve the symptoms and improve the prognosis of patients with acute heart failure. However, there is no report on the effect of serelaxin on hypoxia/reoxygenation injury of myocardial microvascular endothelial cells (H5V) in mice through regulating oxidative stress. OBJECTIVE: To investigate the role on serelaxin in a model of hypoxia/reoxygenation-induced mouse cardiac microvascular endothelial cells (H5V)injury. METHODS: To establish a mouse H5V hypoxia/reoxygenation injury model, cells were incubated under hypoxia for 3, 6, and 12 hours followed by 3 hours of reoxygenation. The optimal time of cellular hypoxia/reoxygenation was determined by cell viability and lactate dehydrogenase activity assay. Different concentrations of serelaxin (0, 60, 100, 140, 180 ng/mL) were selected to act in the H5V cellular hypoxia/reoxygenation injury model, and the optimal drug concentration for drug treatment of cellular hypoxia-reoxygenation injury was determined by the cell viability and lactate dehydrogenase activity assays. H5V cells were then divided into three groups: control, model (hypoxia/reoxygenation), and serelaxin (hypoxia/reoxygenation+180 ng/mL serelaxin) groups. Malondialdehyde, superoxide dismutase and reactive oxygen species levels were detected in each group. RESULTS AND CONCLUSION: Compared with the control group, cell proliferation was significantly reduced after hypoxia 6 hours/reoxygenation 3 hours and hypoxia 12 hours/reoxygenation 3 hours (P < 0.05), while the lactate dehydrogenase activity in cell supernatant was significantly increased (P < 0.05). Therefore, cells cultured under hypoxia 6 hours/reoxygenation 3 hours were selected for subsequent experiments. Cell viability was significantly increased in the serelaxin group compared with the model group (P < 0.05), while the lactate dehydrogenase activity in cell supernatant was significantly decreased (P < 0.05). Therefore, 180 ng/mL serelaxin was selected for subsequent experiments. Compared with the model group, the serelaxin group showed a significant decrease in reactive oxygen species and malondialdehyde levels but a significant increase in the level of superoxide dismutase (all P < 0.05). To conclude, H5V cells subjected to hypoxia 6 hours/reoxygenation 3 hours could establish a model of H5V vascular endothelial cell injury, and 180ng/mL serelaxin could improve the cell viability and anti-oxidative stress ability. [ABSTRACT FROM AUTHOR]