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Objective To investigate the effect of NOD-like receptor family pyrin domain containing 3（NLRP3) knockdown on a mouse model of nonalcoholic steatohepatitis (NASH) induced by high-fat high-carbohydrate (HFHC) diet. Methods A total of 44 mice were randomly divided into normal diet group (CON group) with 20 mice and HFHC group with 24 mice. At the end of week 14 of modeling，4 mice were randomly selected from the HFHC group for the pre-experiment of adeno-associated virus (AAV) by tail vein injection, and NLRP3 knockdown was verified after 4 weeks. After NLRP3 knockdown was verified at the end of week 18, the remaining 40 mice were given a single tail vein injection of AAV, and then they were divided into CON+NLRP3 knockdown negative control group (CON+NLRP3-NC group), CON+NLRP3 knockdown group (CON+NLRP3-KD group), HFHC+NLRP3- NC group, and HFHC+NLRP3-KD group, with 10 mice in each group. At the end of week 24, the activation of NLRP3 inflammasome was observed； related indicators were measured, including body weight, liver weight, liver index, and glucose metabolism (fasting blood glucose, fasting insulin, and Homeostasis Model Assessment of Insulin Resistance [HOMA-IR] index)；the indicators of liver lipid content (liver triglyceride [TG] and oil red O staining), liver inflammation (serum alanine aminotransferase [ALT] activity, HE staining, and inflammation-related genes), and liver fibrosis (Sirius Red staining and fibrosis-related genes) were measured. A one-way analysis of variance was used for comparison of continuous data between multiple groups, and the least significant difference t-test was used for further comparison between two groups. Results Compared with the CON+NLRP3-NC group based on the results of Western Blot, the HFHC+NLRP3-NC group had significant increases in the protein expression levels of NLRP3, pro-Caspase1, Caspase1, ASC, and IL-1β, while the HFHC+NLRP3-KD group had significant reductions in these levels (all P<0.05). The HFHC+NLRP3-NC group showed varying degrees of increase in body weight, liver weight, liver index, and glucose metabolism indicators, while the HFHC+NLRP3-KD group showed significant improvements in these indicators (all P<0.05). As for hepatic fat deposition, compared with the CON+NLRP3-NC group, the HFHC+NLRP3-NC group had a significant increase in liver TG, with a large number of red lipid droplets shown by oil red O staining, and the HFHC+ NLRP3-KD group had significant reductions in liver TG and the number of lipid droplets in the liver (all P<0.01). In terms of liver inflammation, compared with the CON+NLRP3-NC group, the HFHC+NLRP3-NC group had significant increases in serum ALT, NAFLD activity score, and inflammation-related genes, while the HFHC+NLRP3-KD group had significant reductions in these indicators (all P<0.01). As for liver fibrosis, compared with the CON+NLRP3-NC group, the HFHC+NLRP3-NC group had significant increases in collagen fiber area and fibrosis-related genes, and the HFHC+NLRP3-KD group had significant reductions in fibrosis-related genes (all P<0.05) and a tendency of reduction in collagen fiber area (P>0.05). Conclusion NLRP3 knockdown can significantly improve hepatic fat deposition and inflammation in a mouse model of HFHC-induced NASH. [ABSTRACT FROM AUTHOR]

Chemistry is no longer a pure experimental science. Theoretical calculations and chemical experiments can be used to study the properties of compounds and chemical reactions from different perspectives. Now, calculation is an important branch of chemical research. In this manuscript, through a specific calculation study on the mechanism of CO2 functionalization reduction reaction of aniline, the basic methods and processes of quantum calculation are introduced and some reaction details that are easily overlooked or cannot be discovered during the experimental process are discovered by analyzing the calculation results. [ABSTRACT FROM AUTHOR]

目的 研究医院感染对肿瘤患者住院费用的影响, 为进一步加强医院感染管理提供数据支持。方法 选取某肿瘤专科医院2020年1月1日至12月31日47 648例住院患者作为研究对象, 应用SPSS25.0统计软件对医院感染患者和非医院感染患者进行1∶1 CCM匹配, 探讨医院感染对平均住院日、住院费用的影响。结果 2组患者比较显示, 每发生1例医院感染住院时间日增11.67 d、总住院费用增加31 803.80元, 其中西药费增加11 294.03元、治疗费增加5 161.45元, 差异均有统计学意义（P＜0.05）。2组患者位居前4位的感染部位依次为下呼吸道、血液、手术部位和泌尿道, 每发生1例上述部位感染, 住院费用依次增加41 088.77、17 654.40、24 609.56、27 834.30元, 差异均有统计学意义（P＜0.05）。除＞30～40年龄段以外, 其余各年龄段发生医院感染均使住院费用明显增加, 差异均有统计学意义（P＜0.05）。年龄越大, 发生医院感染所增加住院费用越多。结论 医院感染可导致肿瘤患者平均住院日和住院费用增加, 应进一步强化医院感染目标性监测和精准防控。 [ABSTRACT FROM AUTHOR]

This paper aims to understand the quality of the notifiable communicable disease epidemic report of primary medical institutions in Anhui Province in 2021 by conducting a comprehensive evaluation that adopts the fuzzy combination application of entropy weight TOPSIS and RSR method. The results showed that the quality of notifiable communicable disease epidemic reports was the highest in southern Anhui and the lowest in northern Anhui. Among the prefecture ⁃level cities in this evaluation, Anqing and Wuhu ranked the highest, while the bottom two were Lu’an and Xuancheng. The comprehensive quality of notifiable communicable disease epidemic reports decreased in the following order：community health service centers, township hospitals, other institutions like clinics and outpatient departments, and village clinics. The legal management of notifiable communicable disease epidemic reports of primary healthcare institutions in Anhui Province needs to be improved. Primary healthcare institutions should standardize the establishment of notifiable communicable disease epidemic reporting departments, appointing full⁃time personnel to manage the reporting. We also recommend refining the training mechanism for communicable disease prevention and control personnel, strengthening the coordination among multiple departments including the government, disease control and prevention center, health supervision, and healthcare institutions, while incorporating communicable disease modules into information construction. [ABSTRACT FROM AUTHOR]

BACKGROUND: As a coating material, chitosan can effectively improve biological properties of medical metal surfaces due to its excellent film-forming and metal binding abilities. However, the biological activity and osteoinductive ability of chitosan are insufficient. Graphene oxide can improve the mechanical properties and biocompatibility of various polymer materials, as well as promote osteogenic differentiation. The combination of chitosan and graphene oxide may have better biological properties and osteogenic activity. OBJECTIVE: To construct the graphene oxide-modified chitosan composite coating material, analyze the surface morphology, chemical composition, and physical properties of the coating material, as well as the effects of the coating materials on proliferation, adhesion, and osteogenic differentiation of osteoblasts. METHODS: The titanium surface was treated with 3-aminopropyltriethoxysilane to form silane groups on the surface, and then glutaraldehyde was used to cross-link chitosan with silane groups to prepare simple chitosan coating and graphene oxide/chitosan composite coating, respectively. The surface morphology, chemical structure and hydrophilic properties of the coatings were characterized by atomic force microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, and contact angle measurement system. Rat osteoblasts were seeded on the surface of the two materials, and the proliferation, spreading, and osteogenic differentiation of rat osteoblasts were analyzed by CCK-8 assay, scanning electron microscopy, and reverse transcription-polymerase chain reaction analysis. RESULTS AND CONCLUSION: (1) The thickness of the graphene oxide sheets was about 2 nm as measured by atomic force microscopy; the scanning electron microscopy showed that the surfaces of both coatings were smooth and dense, in which the chitosan molecules were closely arranged and the graphene oxide was uniformly distributed in the chitosan, while wrinkles appeared on the surface of chitosan due to the addition of grapheme. The contact angle of the graphene oxide-chitosan composite coating was significantly smaller than the chitosan coating alone (P < 0.05). (2) CCK-8 assay showed that the graphene oxide-chitosan composite coating better promoted the proliferation of osteoblasts compared with the chitosan coating alone. (3) The scanning electron microscopy showed that better spreading was found in osteoblasts on the surface of the graphene oxide-chitosan composite coating compared with the chitosan coating alone. (4) Reverse transcription-polymerase chain reaction analysis showed that the mRNA expression of alkaline phosphatase and Runx2 was significantly increased in the graphene oxide/chitosan composite coating group compared with in the chitosan coating alone group (P < 0.05). (5) These findings suggest that graphene oxide/chitosan composite coating had good biocompatibility, physicochemical, and osteoinductive properties. [ABSTRACT FROM AUTHOR]

Aiming at the problem that there is abnormal data during the process of collecting data from centrifugal pumps. The causes of abnormal data in the process of collecting data from centrifugal pumps, the optimization of the generative adversarial network（GAN） and the method of abnormal data detection were studied. A method for anomaly detection of centrifugal pump timing data using generative adversarial networks had been proposed（this method could optimize generative adversarial networks to solve the problem of gradient vanishing）. Firstly, the basic model in the framework of the GAN was established by using the long short-term memory neural network, and the temporal correlation of the capture data distribution was enhanced, and the problem of gradient disappearance was solved by using the Wasserstein distance method. Then, a centrifugal pump abnormal data detection experiment bench was built to collect data during the operation of the centrifugal pump and the reasons for the abnormal data were analyzed. Finally, the generator and discriminator of the GAN were trained based on normal data, and the loss score was constructed as a threshold to detect abnormal data by using reconstruction loss and discrimination loss. The research results show that the performance of the GAN in data anomaly detection is better than other unsupervised learning anomaly data detection algorithms such as isolated forest, auto-encoder（AE）, K-Means. The GAN can detect abnormal data of centrifugal pump with an accuracy rate of 89.5%, this method can effectively detect abnormal timing data of centrifugal pumps, achieving the goal of optimizing the database and improving the accuracy of rotating machinery fault diagnosis. In conclusion, the study provides a novel approach to detecting abnormal data during the process of collecting centrifugal pump data. [ABSTRACT FROM AUTHOR]

Practical teaching is an essential teaching link for applied disciplines, and collaborative education of industry-university-research effectively integrates resources such as education and scientific research to collaborate and cultivate talents. The applied chemistry major of Fujian Normal University is supported by industry-universityresearch projects, with reform measures such as built campus practical teaching platforms and established practical teaching bases. With the support of practical teaching after the reform, students' comprehensive application, practical ability, innovative thinking have been significantly improved. Students directly connected with the needs of enterprises should be cultivated to achieve a win-win situation for both schools and enterprises. [ABSTRACT FROM AUTHOR]

Personnel training is the foundation of Healthy Chin逅 and the new era has put fonvard high-quality requirements for the training of high-level applied public health personneL This study aims to construct the path and measures of domestic high-level applied public health personnel, in order to provide reference for the talent training in China. Through literature research on public health education in China and the United States, this study summarizes the background, current situation, and connotation of the training of high-level applied public health talents in China, draws on the experience and inspirations of the United States, and raises suggestions on the development of China's training of high-level applied public health personneL China can carry out the training of high-level applied public health talents from the perspective of macro-social ecology and micro-path. For the specific micro-path, we can start with educational concept, training plan, teaching staffs training mode, and evaluation mechanism to improve the quality of public health education. [ABSTRACT FROM AUTHOR]

Objective To investigate the prevalence of hyperhomocysteinemia (HHcy) its association with different types of obesity in middle-aged and elderly people in agricultural and pastoral areas of Tibet. Methods By using the method of stratified cluster sampling, the permanent residents over 40 years old in three counties/districts of Lhasa were selected as the survey subjects. Conditional logistic regression model was used to analyze the relationship between different types of obesity and hyperhomocysteinemia. Results A total of 2 951 cases were included in the analysis, and the prevalence rate of HHcy in agricultural and pastoral areas of Tibet was 67.6%. Participants of men, older-age} lower income level and frequency of fruit consumption were more likely to have HHcy. Taking the normal weight + non-central obesity group as the reference group, the general obesity + central obesity group increased the risk of HHcy by 55% (OR二L55, 95%CI: 1.16-2.08) and 51% (OR=L51, 95%CI: 1.17-1.96), respectively, and those who were ovenveight and central obesity had a 48% increased risk of HHcy (OR=L4& 95%CI: 1.14-1.92), respectively. Conclusion The prevalence of HHcy is high among residents in agricultural and pastoral areas of Tibet, and those who suffer from both general obesity and central obesity have a higher risk of HHcy. [ABSTRACT FROM AUTHOR]

Objective To solve the exposure -response model of digestive tract tumor of chrysotile workers by iterative reweighted least square method (IRLS) and Bayesian method, to provide reference for health protection of chrysotile workers. Methods Using the cohort data of 1 929 workers in a chrysotile asbestos mine in western China from 1981 to 2014 as the training set, IRLS and Bayesian methods were used to solve the exposure -response model of gastrointestinal tumors of chrysotile workers based on Poisson generalized linear model. Results The observed data were randomly divided into training set and validation set, and the model was solved by IRLS and Bayesian method, respectively. The average absolute error of the model solved by Bayesian method was smaller. The model prediction showed that the exposure concentration of chrysotile asbestos had a significant positive correlation with the number of digestive tract tumors (the average value of chrysotile asbestos exposure was 1.500, 95% HDI: 0.927-2,122), and the growth rate of the number of digestive tract tumors increased with the increase of exposure concentration. Conclusion In the case of small amount of data, Bayesian method is more suitable than IRLS method to solve the digestive tract tumor exposure-response model of chrysotile workers. This study supports that chiysotile asbestos exposure can increase the risk of digestive tract tumors, and this model can provide numerical reference for the risk monitoring and prevention of digestive tract tumors in chrysotile workers. [ABSTRACT FROM AUTHOR]

Objective To preliminarily investigate the role of the Ras‑related C3 botulinum toxin substrate 3 (RAC3) in type 1 diabetes mellitus (T1DM) and dendritic cells (DC). Methods Patients with T1DM were enrolled from the Guangdong Type 1 Diabetes Translational Medicine Study from 2011 to 2016. Healthy controls were volunteers with normal glucose tolerance who visited the Third Affiliated Hospital of Sun Yat‑sen University from 2011 to 2016. Whole exome sequencing was performed in the screening stage, and genotyping was performed in the validation stage among all participants. CRISPR/Cas9 gene editing technology was used to construct the RAC3 whole‑body knock‑out (KO) mouse model on C57BL/6 mouse background. Polymerase chain reaction (PCR), quantitative real‑time PCR (qRT‑PCR), and Western blotting assay were used to verify that RAC3 was knocked out on DNA, RNA and protein level. Flow cytometry was used to compare the differentiation ratio of DC and the expression levels of maturation and activation indicators, such as major histocompatibility complex‑Ⅱ (MHC Ⅱ), CD86 and CD80, between RAC3 KO mice and wild‑type (WT) littermate control mice (3 mice in each group). Logistic regression was used to compare the frequency distributions of the alleles between case and control groups. Intergroup comparisons were conducted by independent‑samples t‑tests. Results A total of 72 patients with T1DM and 487 healthy controls were included in the screening stage, and 122 patients with T1DM and 577 healthy controls were included in the validation stage. The results in the screening stage showed that the frequencies of the T allele of RAC3 rs4969478 in patients with T1DM were higher than that in healthy controls [7.64% (11/144) and 1.13% (11/974), respectively, OR=9.38, P<0.001]. The results in the validation stage also showed that the frequencies of the T allele of RAC3 rs4969478 in patients with T1DM were increased compared to the healthy controls [4.50% (11/244) vs. 1.13% (13/1 154), respectively, OR=4.14, P<0.01]. There was no significant difference in the percentage of differentiated DC between WT mice and RAC3 KO mice (85.6%±1.1% vs. 83.3%±0.25%, respectively, P=0.08). There was no significant difference in the expression levels of maturation and activation indicators (such as MHC Ⅱ, CD86 and CD80) in DC between WT mice and RAC3 KO mice (P>0.05). Conclusion Genetic studies suggest that RAC3 may be a susceptibility gene for human T1DM, but it may not be involved in the pathogenesis of T1DM via DC. [ABSTRACT FROM AUTHOR]

Objective To explore the metabolite profile and metabolic pathways of newly diagnosed multiple myeloma (MM). Methods Gas chromatography-mass spectrometry (GC-MS) was employed for the high-throughput detection and identification of serum samples from 55 patients with MM and 37 healthy controls matched for age and sex from 2016 to 2017 collected at the First Affiliated Hospital of Soochow University. The relative standard deviation (RSD) of quality control (QC) samples was employed to validate the reproducibility of GC-MS approach. The differential metabolites between patients with MM and healthy controls were detected by partial least squares discrimination analysis ( PLS-DA), and t-test with false discovery rate ( FDR) correction. Metabolomics pathway analysis ( MetPA) was employed to construct metabolic pathways. Results There were 55 MM patients, including 34 males and 21 females. The median age was 60 years old (42-73 years old). There were 30 cases of IgG type, 9 cases of IgA type, 1 case of IgM type, 2 cases of non-secreted type, 1 case of double clone type and 12 cases of light chain type, including 3 cases of kappa light chain type and 9 cases of lambda light chain type. The result of QC sample test showed that the proportion of compounds with the RSD of the relative content of metabolites < 15% was 70.21% obtained by the reproducibility of GC-MS experimental data, which implied that the experimental data were reliable. A total of 17 metabolites were screened differently with the healthy control group, including myristic acid, hydroxyproline, cysteine, palmitic acid, L-leucine, stearic acid, methionine, phenylalanine, glycerin, serine, isoleucine, tyrosine, valine, citric acid, inositol, threonine, and oxalic acid (VIP>1, P< 0.05). Metabolic pathway analysis suggested that metabolic disorders in MM patients comprised mainly phenylalanine metabolism, glyoxylic acid and dicarboxylic acid metabolism, phosphoinositide metabolism, cysteine and methionine metabolism, glycerolipid metabolism, glycine, serine, and threonine metabolism. Conclusion Compared with normal people, patients with newly diagnosed MM have obvious differences in metabolic profiles and metabolic pathways. [ABSTRACT FROM AUTHOR]

[ Objective/Meaning ] Based on the actual situation of the old revolutionary base areas, the path to give play to the advantages of red culture was explored, in order to provide beneficial theoretical and practical reference for the development of rural areas in the old revolutionary base areas and the realization of common prosperity.[ Methods/Procedures ] On the basis of field investigation, through the literature research and the analysis of induction, the role of red cultural resources on the rural development in the old revolutionary base areas was analyzed. Then, the value implication of developing the red culture to realize the common prosperity of the whole people, the comprehensive common prosperity, the co-construction common prosperity, and the gradual common prosperity in the old revolutionary base areas was summarized. And the realistic predicament of the development of rural red culture in the old revolutionary base areas was analyzed. Finally, the corresponding countermeasures and suggestions were put forward.[ Results/Conclusions ] There were some practical difficulties in the development of rural red culture in the old revolutionary base areas, such as the weak economic foundation, the remote geographical location, the lagging cultural construction, and the difficulties in grassroots governance. It was necessary to promote the red culture to boost the common prosperity of the old revolutionary base areas through the practical paths including creating the characteristic brand industry, building the red “nostalgic” villages, carrying forward the spirit of the old revolutionary base areas, and improving the ability of thematic governance. [ABSTRACT FROM AUTHOR]

As the background noise content in the actual measurement of horizontal centrifugal pump was large, and the fault characteristics were often submerged, resulting in poor mechanical fault diagnosis effect. In order to obtain its operation state and fault diagnosis in current time and accurately, a mechanical fault diagnosis method of horizontal centrifugal pump based on complementary ensemble empirical mode decomposition（CEEMD）-optimized k nearest neighbor（KNN） was proposed. Firstly, the mechanical fault acceleration signal of horizontal centrifugal pump was collected, and the signal was decomposed once by CEEMD to obtain the intrinsicmode function（IMF）. The correlation coefficient of IMF was obtained by the correlation coefficient method to determine the correlation component and uncorrelated component. Secondly, the uncorrelated component was processed by the improved wavelet threshold denoising method to extract the time-frequency fault features that could be analyzed by the reconstructed signal. Finally, a centrifugal pump experimental bench was built, and the above fault diagnosis methods were used to classify and diagnose the mechanical faults of the centrifugal pump.The results show that the signal-to-noise ratio（SNR） of the signal evaluation index after CEEMD noise reduction is 2.257 1, which is 0.438 1 higher than the original denoising method. After optimization, the accuracy of KNN classification for mechanical fault diagnosis of horizontal centrifugal pump can reach 96.7%, which can effectively identify faults and achieve the purpose of intelligent diagnosis. [ABSTRACT FROM AUTHOR]

The comprehensive evaluation mode of the classified supervision and control of infectious diseases was an innovative measure to improve the level of supervision and management of infectious disease prevention and control in medical and health institutions at this stage in China. Since the pilot of this model in 2014，it has not only achieved remarkable results but also exposed the problems in the supervision and management of infectious diseases in China’s medical and health institutions. On the basis of summarizing the current situation of the implementation status of the comprehensive evaluation model，this paper analyzed the current situation and progression of the mode by using the SWOT（strengths weaknesses opportunities threats） method for strategic management. This study also discussed the strengths，weaknesses，opportunities and threats of the internal and external environment faced during the development of the model. It also proposes development strategies and policy recommendations according to the SWOT analysis matrix to provide a reference for effectively improving the level of the supervision and management of infectious disease prevention and control. [ABSTRACT FROM AUTHOR]

目的 分析湖北省恩施市乡镇卫生院的运行效率、全要素生产率及其分解指数，为少数民族地区乡镇卫生院效率改进提供参考。方法 收集恩施市 17 家乡镇卫生院 2016—2019 年的投入、产出指标面板数据, 利用 Bootstrap-DEA 方法测算卫生院的运行效率, 利用 Bootstrap-Malmquist-DEA 方法测算卫生院全要素生产率及其分解指数。结果 2016—2019 年乡镇卫生院经 Bootstrap-DEA 纠偏后的效率值均低于纠偏前, 纠偏后效率值属于“优秀” (≥ 0.900且＜1.000) 的乡镇卫生院分别有4、12、15、5 家。2016—2019 年乡镇卫生院全要素生产率降低15.3%, 其中技术进步指数下降 17.8%, 技术效率上升 3.1%, 纯技术效率下降 0.2%, 规模效率增长 3.3%。结论 恩施市 17 家乡镇卫生院运行效率差异明显, 整体技术水平偏低。建议恩施市在重视卫生资源投入的同时, 加强先进医疗技术的引进与优秀卫生人才的培养, 提升乡镇卫生院综合服务能力。 [ABSTRACT FROM AUTHOR]

Objective: To investigate and analysis the correlation between the expression levels of serum Interferon-γ (IFN-γ), erythrocyte sedimentation rate (ESR) and soluble interleukin receptor (sIL-2R) in children with acute lymphoblastic leukemia (ALL). Methods: From February 2017 to October 2020, 62 cases of children with acute lymphoblastic leukemia who were diagnosed and treated in the Department of Hematology of our hospital were selected as the ALL group, and the other 62 cases of healthy children who had physical examination in our hospital during the same period were selected as the control group. The serum IFN-γ, ESR, and sIL-2R levels were used to determine the immunophenotype and chromosome analysis of children in the ALL group, followed up the prognosis of the children and given correlation analysis. Results: The proportions of CD34+, CD117+, and karyotype abnormalities in the ALL group were 77.4 %, 58.1 %, 54.8 %, respectively, which were higher than those of the control group (27.4 %, 17.7 %, 14.5 %)(P<0.05). The levels of serum ESR and sIL-2R in the ALL group were higher than those in the control group, and the levels of IFN-γ were lower than those in the control group (P<0.05). In children in the ALL group, followed-up until December 1, 2021, the average follow-up time were 29.14± 4.28 months, the overall survival time were 18.29± 1.22 months, and the event-free survival time were 10.48± 0.33 months; there were 22 cases were died and 40 cases were survived, the mortality rate were 35.5 %. Spearman correlation analysis showed that serum IFN-γ, ESR, sIL-2R levels were correlated with CD34+ positive, CD117+ positive, abnormal chromosomal karyotype, overall survival time, event-free survival time (P<0.05). Multivariate logistic regression analysis showed that IFN-γ, ESR, sIL-2R were important factors affected the prognosis and death of children with acute lymphoblastic leukemia (P<0.05). Receiver operating characteristic curve (ROC) showed that the area under the curve of IFN-γ, ESR, sIL-2R predicted the prognostic death of children with acute lymphoblastic leukemia were 0.867, 0.735, and 0.796, respectively. Conclusion: Children with acute lymphoblastic leukemia are mostly accompanied by high expression of serum IFN-γ and low expression of serum ESR and sIL-2R. All three of them are related to the immune phenotype and chromosome karyotype of the child, and are also the important factors for the prognosis of children. [ABSTRACT FROM AUTHOR]

Liver fibrosis is the common consequence of various chronic liver injuries and is mainly characterized by the imbalance between the production and degradation of extracellular matrix, which leads to the accumulation of interstitial collagen and other matrix components. Matrix metalloproteinases ( MMPs) and their specific inhibitors, i.e., tissue inhibitors of metalloproteinases ( TIMPs), play a crucial role in collagen synthesis and lysis. Through a literature review, this article reviews the experimental studies of liver fibrosis based on MMPs/ TIMPs, summarizes the components that may exert an anti - liver fibrosis effect by affecting the expression or activity of MMPs/TIMPs, and attempts to clarify the mechanism of MMPs/TIMPs in regulating collagen homeostasis, so as to provide support for the development of anti - liver fibrosis drugs. [ABSTRACT FROM AUTHOR]

Objective　To investigate the correlation of pulmonary function indexes and bronchial wall thickness measured as computed with high resolution computed tomography in patients with chronic obstructive pulmonary diseases. Methods　Eighty COPD patients were assigned to four groups based on the new COPD Global Initiative Classification, meanwhile，fifty normal people with physical examination in the hospital were selected as controls simultaneously. All subjects underwent chest HRCT scanning to measure the bronchial wall thickness at the bilateral apical bronchial opening，and the correlation between the bronchial wall thickness and lung function was studied. Results　Bronbronchial wall thickness was significantly increased in COPD patients compared with normal population，and the difference was statistically significant （ P < 0.05）. From the mild group to the very severe group， the bilateral bronchial wall thickness gradually increased，FEV1，FVC，FEV1/FVC gradually decreased，with statistically significant differences （ P < 0.05） . Correlation analysis： bilateral bronchial wall thickness was significantly inversely associated with each index of lung function （ P < 0.05）. Conclusions　HRCT can be used to measure bronchial wall thickness and evaluate the degree of bronchial wall remodeling in COPD patients. With the deepening of the disease grade，the thickness of the bronchial wall of COPD patients gradually increased. There is a significant correlation between lung function indexes and bronchial wall thickness，that is，the thickening of bronchial wall，the decline of lung function indexes and the deterioration of lung function. HRCT measurement of bronchial wall thickness in COPD patients has clinical significance for the evaluation of the severity of the disease. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To probe into the effects of Zishen Tiaochong method and growth hormone on outcome of in vitro fertilization and embryo transfer (IVF-ET) in women with diminished ovarian reserve (DOR) at different age. METHODS: Pregnancy outcomes of 217 patients with DOR undergoing IVF-ET in Guangdong Women and Children Hospital from Jan. 2017 to Jan. 2019 were analyzed retrospectively, including 105 patients < 35 years old and 112 patients ≥35 years old. All patients were on antagonist regimens and were on the first cycle of superovulation. In each age group, according to different pretreatment methods, all patients were divided into the traditional Chinese medicine group, the GH group and the control group. The traditional Chinese medicine group was given intervention of Zishen Tiaochong method, the GH group was pretreated with GH, and the control group was not pretreated with TCM or GH. Differences in clinical and laboratory indexes and pregnancy outcomes were observed in the three groups within the two age groups. RESULTS: There was no significant change in ovarian response and clinical outcome in patients with DOR aged <35 years old after GH intervention. The thickness of intrauterine membrane on the day of transplantation, number of harvested eggs and two prokaryotes (2PN) fertilization rate of the traditional Chinese medicine group were slightly higher than those of the GH group and the control group, but the difference was not statistically significant (P>0. 05). For patients with DOR aged ≥35 years old, the total application time and total dose of Gonadotropin (Gn) in the CH group were significantly lower than those in the traditional Chinese medicine group and the control group; the optimal embryo rate in the GH group was significantly higher than that in the traditional Chinese medicine group and the control group, with statistically significant difference (P<0. 05). Regardless of patients aged ≥35 years old or aged <35 years old, the endometrial thickness of patients in the traditional Chinese medicine group was the lowest during menstruation, which was significantly different from the GH group and the control group (P<0. 05). Within the two age groups, there was no statistically significant difference in the implantation rate, pregnancy rate and clinical pregnancy rate in the traditional Chinese medicine group, CH group and the control group, the difference was not statistically significant (P>0. 05). CONCLUSIONS: The intervention of GH in the elderly (≥35 years old) patients with DOR can improve the ovarian reactivity to a certain extent, while Zishen Tiaochong method is more effective in younger (< 35 years old) patients with DOR, and Zishen Tiaochong method can promote the endometrium spallation during menstruation and increase the endometrium thickness on the day of transplantation. [ABSTRACT FROM AUTHOR]

Intrahepatic cholestasis (IHC) is a dysfunction of bile production, secretion, and excretion caused by various factors including genetics, immunity, inflammation, stones, and tumors, and therefore, the bile is not normally discharged into the duodenum and reversely flows into blood, which causes a series of clinical diseases with organic damage, metabolic disorders, and dysfunction. In recent years, with the deepening of clinical and basic research, intestinal microecology is found to play an important role in the development and progression of liver diseases. This article reviews the mechanism of action of intestinal microecology and inflammatory factors in intrahepatic cholestasis and explores the research advances in the role of probiotics in regulating intestinal flora stability and improving intestinal microenvironment, so as to provide a basis for clinical treatment, scientific research, and drug research and development. [ABSTRACT FROM AUTHOR]

BACKGROUND: Studies have found that Telocyte interstitial cells may play a role in heart diseases, such as acute myocardial infarction, atrial fibrillation, valvular heart disease and myocardial amyloidosis, but the mechanism is unclear. The basic research of cardiac Telocyte interstitial cells is of important clinical value. OBJECTIVE: To explore the existence and characteristics of Telocyte interstitial cells in the mouse heart. METHODS: Fifty-nine Kunming mice aged from 10 days to 10 weeks (provided by Fuzhou Wu’s Experimental Animal Center in China) were chosen. The gastrointestinal tract Cajal interstitial cells and bladder Telocyte interstitial cells were used as controls. ACK2 (c-kit monoclonal antibody) was used as an indicator for the immunohistochemical staining of myocardial tissue in 13 mice. Cardiac Telocyte cells from another 41 mice were isolated, cultured in vitro and observed for cell morphology and protrusion under immunofluorescence laser confocal microscope. Ultrastructure of Cajal and Telocyte interstitial cells from the remaining five mice was observed by transmission electron microscopy. RESULTS AND CONCLUSION: (1) Positive expression of c-kit was found in the gastrointestinal and bladder tissues by immunohistochemical staining, but not in the cardiac tissues. However, in vitro cultured cells were positive for c-kit. (2) Under the immunofluorescence laser confocal microscope and transmission electron microscope, the cardiac Telocyte cells with spindle or irregular bodies were discovered in myocardial fibers or around the capillaries, and dense strips were found between cell protrusions from cardiac Telocyte cells and the capillaries. There was no difference in the shape of cell body and the number of cell protrusion compared with the control group, but a significant difference in the length of cell protrusions was found (P < 0.05). In conclusion, the presence of Telocyte cells with positive expression of c-kit in the mouse heart is confirmed by primary culture, immunofluorescence staining and transmission electron microscopy. The body size and number of cell protrusions are similar to those of gastrointestinal tract Cajal interstitial cells or bladder Telocyte interstitial cells. Ventricular Telocyte interstitial cell protrusion is the longest, atrial Telocyte interstitial cell protrusion is the shortest, and ventricular Telocyte interstitial cell protrusion is tightly connected with the capillaries. [ABSTRACT FROM AUTHOR]

Objective To investigate whether menaquinone-4 （MK-4） can exert a protective effect against carbon tetrachloride （CCl4）-induced acute liver injury （ALI） in mice by alleviating ferroptosis. Methods After adaptive feeding， adult male ICR mice， aged 8 weeks， were divided into Control group， MK-4 group， CCl4 model group （6-hour， 12-hour， and 24-hour）， and MK-4+CCl4 group （6-hour， 12-hour， and 24-hour）， with 6 mice in each group. The mice in the Control group were given intraperitoneal injection of an equal dose of corn oil； the mice in the MK-4 group were given intraperitoneal injection of 40 mg/kg MK-4 solution， followed by an equal dose of corn oil after 1 hour； the mice in the MK-4+CCl4 group （6-hour， 12-hour， and 24-hour） were given intraperitoneal injection of 40 mg/kg MK-4 solution， and after 1 hour， the mice in this group and the CCl4 model group （6-hour， 12-hour， and 24-hour） were given intraperitoneal injection of 0.3 mL/kg CCl4 solution， with samples collected at 6， 12， and 24 hours. HE staining was used to observe the pathological changes of mouse liver； Prussian blue staining was used to observe iron accumulation in liver tissue； a biochemical analyzer was used to measure the serum levels of aspartate aminotransferase （AST） and alanine aminotransferase （ALT）； related kits were used to measure the levels of tissue iron content and the oxidative stress indices malondialdehyde （MDA） and glutathione （GSH） in liver homogenate； RT-PCR was used to measure the expression levels of ferroptosis marker genes （acyl-CoA synthetase long-chain family member 4 ［ACSL4］， prostaglandin-endoperoxide synthase 2 ［PTGS2］， and glutathione peroxidase 4 ［GPX4］） and iron metabolism-related genes （hemojuvelin ［HJV］， transferrin receptor 1 ［TFR1］， and ferroportin ［FPN］）， and Western blot was used to measure the protein expression level of GPX4. A one-way analysis of variance was used for comparison of continuous data between multiple groups， and the least significant difference t-test was used for further comparison between two groups. Results In the aging study， compared with the Control group， the CCl4 model group （6-hour， 12-hour， and 24-hour） had significant increases in liver weight coefficient and the serum levels of ALT and AST （all P<0.05）， and HE staining also showed that liver injury gradually aggravated over time. Meanwhile， compared with the CCl4 model group （6-hour， 12-hour， and 24-hour）， the MK-4+CCl4 （12-hour） group had significant reductions in liver weight coefficient and the serum levels of ALT and AST （all P<0.05）， with a reduction in the necrotic area of liver tissue， and therefore， 12-hour mouse tissue samples were used for detection in the following study. Compared with the Control group， the CCl4 group had a significant increase in MDA and a significant reduction in GSH （both P<0.05）， and compared with the CCl4 group， the MK-4+CCl4 group had a significant reduction in MDA and a significant increase in GSH （both P<0.05）. Compared with the Control group， the CCl4 group had significant increases in the key ferroptosis indices ASCL4 and PTGS2 and a significant reduction in GPX4 （all P<0.05）； compared with the CCl4 group， the MK-4+CCl4 group had significant reductions in the mRNA expression levels of ASCL4 and PTGS2 and a significant increase in the mRNA expression level of GPX4 （all P<0.05）. Western blotting showed that compared with the Control group， the CCl4 group had a significant reduction in the protein expression level of GPX4 （P<0.05）， and compared with the CCl4 group， the MK-4+CCl4 group had a significant increase in the protein expression level of GPX4 （P<0.05）. Prussian blue staining showed that compared with the Control group， the CCl4 group had a significant increase in iron accumulation； after MK-4 intervention， compared with the CCl4 group， the MK-4+CCl4 group had a significant reduction in iron accumulation. As for the measurement of iron metabolism genes in mouse liver， compared with the Control group， the CCl4 group had a significant increase in iron content， significant reductions in the mRNA expression levels of FPN and HJV， and a significant increase in the mRNA expression level of TFR1 （all P<0.05）； after protection with MK-4， there was a significant reduction in iron content， significant increases in the mRNA expression levels of FPN and HJV， and a significant reduction in the mRNA expression level of TFR1 （all P<0.05）. Conclusion MK-4 intervention in advance can alleviate CCl4-induced ALI in mice， possibly by inhibiting ferroptosis and improving the expression of iron metabolism-related genes in mouse liver. [ABSTRACT FROM AUTHOR]

The first class serves as the main avenue for cultivating chemistry teachers' quality, while the second class also plays an indispensable role. Using the Chemistry Branch of the Future Outstanding Teachers' Association at Fujian Normal University as a case study, this paper evaluates the feasibility of the association as the second classroom to reinforce teachers' quality training of chemistry normal students. This evaluation encompasses three facets: community systems, community activities and community advantages. In conclusion, we suggest that the college and community level expand incentive policies and reinforce the development of related micro-specializations, offering insights for the training of chemistry normal students in other normal universities. [ABSTRACT FROM AUTHOR]

According to the short of injection volume and high injection pressure in polymer injection block of Shuanghe Oilfield, the characteristics of the polymer injection such as injection speed, the pressure gradient, polymer concentration, the core permeability, as well as the effect of polymer viscosity on injection properties were studied. The experimental results showed that the polymer injection concentration was the most influential factor, and the injection rate was the least one. Rock permeability affected the injection of polymer solutions and propagation in the formation. When core permeability was less than 0.8 μm 2, the rear plugging was greater than the front because of the combined action of mechanical trapping and adsorption. With the core permeability increased (>0.8 μ.m2), polymer solution could be injected further and the plugging mechanism was only adsorption of polymers, which caused minor blockage. At the same time, the pressure gradient at the rear of the core in the polymer flooding was the highest, which indicated that the blocking part mainly occurred in the posterior part of the core. After adjusting the polymer concentration in the field, it was found that the maximum drop rate of the short of injection volume is 35%, which showed that the injection properties of polymer injection wells could be improved effectively. It is a favorable way to increase the pressure and increase the injection. [ABSTRACT FROM AUTHOR]

目的观察利拉鲁肽联合胰岛素强化治疗难治性2型糖尿病的临床价值。方法选取难治性2型糖.病患者76例，将其随机分为观察组和对照组各38例。对照组给予胰岛素强化治疗，观察组给予利拉鲁肽联合.岛素强化治疗。分别于治疗前和治疗后6,12周，记录胰岛素用量，取静脉血检测空腹血糖、糖化血红蛋白、C肽，.算胰岛P细胞功能指标基础胰岛素分泌功能指数（HOMA-β)、胰岛素敏感性指标胰岛素抵抗指数（HOMA-IR)和.岛素作用指数（IAI)，评价临床疗效，记录血糖及血红蛋白均达标时间。结果治疗前两组各指标比较，差异均.统计学意义。治疗后第6、12周，两组胰岛素用量减少、空腹血糖水平和糖化血红蛋白降低（P均<0.05)，HOMA-P、IAI升高而HOMA-IR降低，且观察组较对照组更明显（P均.0.05)。观察组临床总有效率97.37%，高于对.组的81.58%(P<0.05);观察组血糖及血红蛋白均达标时间为（7.85±1.63)(1，短于对照组的（10.95±2.56)(P<0.05)。结论利拉鲁肽联合胰岛素强化治疗难治性2型糖尿病可通过提高胰岛p细胞功能及胰岛素敏感性.从而尽快控制血糖并提高临床疗效。 [ABSTRACT FROM AUTHOR]

结核性胸膜炎是由结核分枝杆菌抗原引起的T淋巴细胞介导的胸膜炎症,其发病机制复杂,至今仍不明确。成熟的T淋巴细胞是一群高度异质性且存在若干亚群的免疫细胞,研究发现结核性胸膜炎患者体内存在Ⅰ型T辅助细胞/Ⅱ型T辅助细胞免疫应答功能紊乱,Th17/Treg失衡及Th9、Th22、黏膜相关恒定T细胞免疫增强现象,CD4+IL-27+T细胞也可通过干预T淋巴细胞亚型的转换来参与疾病的进程。 [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of rosiglitazone injection on cellular immune function and Caspase-3 expression in mice with multiple myeloma. Methods: Multiple myeloma mice were randomly divided into three groups-model group, red blood cell group and rosiglitazone group. The red blood cell group and rosiglitazone group were given tail vein injection of recombinant mouse erythropoietin 5.0 mg/kg and rosiglitazone 5 mg/kg 100 μL, the model group were given equal volume of normal saline via tail vein injection, once a day, the cellular immune function and Caspase-3 expression changes were detected. Results: The tumor volume on the 7th and 14th day of treatment in the three groups were higher than that on the 1st day of treatment (P<0.05), the red blood cell group and rosiglitazone group were lower than the model group(P<0.05), and the rosiglitazone group were lower than the red blood cell group (P<0. 05). The serum levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-琢in the red blood cell group and rosiglitazone group on the 7th and 14th day of treatment were lower than those of the model group (P<0.05), the rosiglitazone group were lower than the red blood cell group (P<0.05). The ratio of splenic B lymphocytes and T lymphocytes on the 14th day and 28th day of treatment between the red blood cell group and the rosiglitazone group were higher than that of the model group (P<0.05), and the rosiglitazone group were higher than the red blood cell group (P<0.05). The expression of Caspase-3 protein in the substantia nigra of the erythrocyte group and rosiglitazone group were lower on the 14th and 28th day of treatment than the model group (P<0.05), and the rosiglitazone group were lower than that of the red blood cell group (P<0.05). Conclusion: The application of rosiglitazone injection in multiple myeloma mice can improve cellular immune function and inhibit the expression of Caspase-3 in the substantia nigra of the brain. At the same time, it can also promote the weight recovery of mice and inhibit the expression of inflammatory factors. [ABSTRACT FROM AUTHOR]

Drug-induced liver injury (DILI) is one of the common adverse drug reactions and is the main cause of withdrawal of drugs after marketing, which has attracted more and more attention of the public, and herb-induced liver injury (HILI) is a special type of DILI. In recent years, the frequent occurrence of HILI not only seriously endangers the health of patients, but also causes the controversy over the safety of traditional Chinese medicine. Therefore, this article reviews the potential risk factors for HILI from the three aspects of "patient", "drug", and "use", so as to provide a basis for the objective identification, prevention, and control of HILI and a reference for the construction of traditional Chinese medicine pharmacovigilance system represented by liver injury. [ABSTRACT FROM AUTHOR]

Inflammasomes play an important role in the innate immunity of the liver; however, the excessive activation of inflammasomes can lead to liver inflammation and injury. The mechanism of nucleotide - binding oligomerization domain - like receptor protein 3 ( NLRP3) inflammasome - mediated liver injury has been extensively studied. Related studies have shown that the development of various liver diseases may be associated with the excessive activation of inflammasomes, especially NLRP3 inflammasome. This article reviews inflammasomes, the activation mechanism of NLRP3 inflammasome, and the role of NLRP3 inflammasome in different liver diseases, so as to provide a reference for the treatment targets of liver diseases from the perspective of NLRP3 inflammasome. [ABSTRACT FROM AUTHOR]

As an important technical lorm ol electrochemical energy storage,lithium ion battery is still limited in practical application due to the possibility ol thermal runaway. Therefore，it is necessary to understand the variation rule ol battery temperature field alter thermal runaway trigger and analyze the factors allecting temperature change. In this paper, ANSYS FLUENT is used to simulate the lithium ion battery, and the internal thermal dillusion law ol the battery is studied by simulating the temperature field change ol the battery under dilierent charge and discharge Iluctuation power input caused by the instability of the generator end. The thermal diffusion law is analyzed by simulating the temperature field alter the thermal runaway triggered by local short circuit in lithium ion battery. The results show that the temperature and wall heat Ilux ol the lithium-ion battery fluctuate with the input current. When triggered by local short circuit, the rate ol thermal runaway extending lengthwise along the electrode is greater than that ol radially spreading outwards. Increasing convective heat transfer coefficient can effectively reduce the rate and amplitude ol battery temperature rise,reducing the possibility ol thermal runaway ol battery. The study ol thermal dillusion law provides a basis for predicting thermal runaway in practical application, improving safety, and improving battery cooling technology. [ABSTRACT FROM AUTHOR]

AIM: To explore the effects of CD38 on lysosome reformation and cholesterol efflux in macrophages. METHODS: Bone marrow-derived macrophages from low-density lipoprotein （LDL） receptor knockout （LDLr-/-） mice were cultured as cell model. Live cell imaging system was applied to evaluate the effect of nicotinic acid adenine dinucleotide phosphate （NAADP） on lysosome number. ELISA was conducted to measure NAADP level in macrophages. After the cells were treated with nicotinic acid （NA）, RT-qPCR was conducted to detect CD38 mRNA expression, and Western blot was conducted to observe CD38 protein expression and phosphorylated transcription factor EB（TFEB） level. Laser scanning confocal microscopy was applied to evaluate the influence of CD38/NAADP signaling on lysosome number and cholesterol egression. RESULTS: NAADP remarkably increased lysosome number （P<0. 05）, and this effect was significantly inhibited by NAADP antagonist NED-19, Ca2+ chelator BAPTA, and calcineurin inhibitor CsA （P<0. 05）. CD38 markedly enhanced NAADP synthesis in macrophages （P<0. 05）. NAADP synthetic substrate NA prominently elevated the expression of CD38 mRNA and protein （P<0. 05）. NA significantly decreased the phosphorylated TFEB level; this effect was also attenuated by NED-19, BAPTA and CsA （P<0. 05）. Disrupting CD38/NAADP signaling pathway markedly inhibited NA-induced enhancement of lysosome number, lysosomal free cholesterol and cytosol cholesterol ester efflux in macrophages （P<0. 05）. NA-induced enhancement of lysosome number, lysosomal free cholesterol and cytosol cholesterol ester efflux abolished in LDLr/CD38 DKO macrophages （P<0. 05）, whereas these effects induced by NA were recovered after CD38 gene rescue. CONCLUSION: CD38 triggers lysosome reformation via TFEB and consequently promotes the efflux of lysosomal free cholesterol and cytosol cholesterol ester. [ABSTRACT FROM AUTHOR]

Decades of research using remotely sensed data have extracted evidence for the presence of an ocean in the northern lowlands of Mars in the Hesperian (∼3.3 Ga), but these claims have remained controversial due to the lack of in situ analysis of the associated geologic unit, the Vastitas Borealis Formation (VBF). The Tianwen-1/Zhurong rover was targeted to land within the VBF near its southern margin and has traversed almost 2 km southward toward the interpreted shoreline. We report here on the first in situ analysis of the VBF that reveals sedimentary structures and features in surface rocks that suggest that the VBF was deposited in a marine environment, providing direct support for the existence of an ancient (Hesperian) ocean on Mars. [ABSTRACT FROM AUTHOR]

Time-sensitive networking flow scheduling algorithms often generate a large number of gate control events, exceeding the capabilities of network devices and making it difficult to deploy scheduling algorithms in practical networks. To address this issue, a novel flexible gate control-based flow scheduling strategy is proposed, which relaxes the strict isolation constraints between real-time traffic flows and best-effort traffic flows, allowing some nodes to not enable the gate control mechanism for real-time flows. This strategy can flexibly select to enable the gate control for real-time business at various network device ports, reducing the gate control events required for scheduling real-time traffic flows by up to 91.6%. It even allows the existence of network devices on the transmission path of real-time traffic that do not support gate control scheduling, achieving a mixed deployment with ordinary networks. [ABSTRACT FROM AUTHOR]

Decades of research using remotely sensed data have extracted evidence for the presence of an ocean in the northern lowlands of Mars in the Hesperian (∼3.3 Ga), but these claims have remained controversial due to the lack of in situ analysis of the associated geologic unit, the Vastitas Borealis Formation (VBF). The Tianwen-1/Zhurong rover was targeted to land within the VBF near its southern margin and has traversed almost 2 km southward toward the interpreted shoreline. We report here on the first in situ analysis of the VBF that reveals sedimentary structures and features in surface rocks that suggest that the VBF was deposited in a marine environment, providing direct support for the existence of an ancient (Hesperian) ocean on Mars. [ABSTRACT FROM AUTHOR]

Due to the fact that graphene possesses high conductivity, large specific surface area and ex- cellent mechanical properties, it has excellent application prospects in the field of supercapacitors. However, when it's directly used as the active electrode materials of supercapacitors, pure graphene face the problem of relatively low specific capacitance. In this paper, 3D porous nanocomposites com- posed of 2D graphene nanosheets, which was modified by OD molybdenum dioxide (MoO2) nanodots, were synthesized by one-step hydrothermal method. And the electrochemical properties of this kind of 0D-2D-3D multi-scale composites were studied as the active electrode materials of supercapacitors. The results showed that the specific capacitance was as high as 499 F/g at 2 mV/s, displaying excellent ca- pacity performance. [ABSTRACT FROM AUTHOR]

Using zinc nitrate hexahydrate and 2-methylimidazole as raw materials and absolute ethanol as solvent, the precursor ZIF-8 was prepared by the mechanical stirring method, and zinc oxide nanorods at different temperatures were prepared by thermal deposition of the precursor, respectively.ZnO-X（X=700,800,900）.The X-ray diffractometer（XRD） patterns showed that the ZnO nanotubes at different temperatures had complete crystallization and high purity.It can be seen from the scanning electron microscope（SEM） that the size distribution of the precursor is 0.5～2 μm; as the thermal deposition temperature increases, the diameter gradually increases from 150 nm to 2 μm, and the length increases from 1 μm to 30 μm.The photocatalytic degradation experiments showed that the degradation efficiencies of ZnO-700,ZnO-800,and ZnO-900 to rhodamine（RhB） were 63.1%,62.1%,and 62.8%,respectively, after 150 min of illumination.The obtained ZnO nanorods have an obvious photocatalytic degradation effect on RhB. [ABSTRACT FROM AUTHOR]

As an important organic chemical raw material, benzil could be synthesized through the oxidization of benzoin by using Salen complexes as catalyst, which is a mature synthetic route but still suffered from issues such as environmentally unfriendly, complicated operation procedures and so on. From the viewpoint of green chemistry, a series of new Salen ligands have been designed and synthesized from salicylaldehyde and ethylene diamine precursors and several Salen complexes [M(Salen)] (17 =Co, Cu, Zn, Ni) were then obtained, which have been successfully used in the catalytic oxidization reaction of benzoin compounds. The investigation data revealed that benzil compounds could be obtained in a high yield of 90. lc/c when benzoin, (Co) Salen catalyst and base (KOH) were mixed in a ratio of I .()(): 0. 08: 1.40 in ethanol at room temperature. Moreover, the (Co) Salen catalyst could be recovered effectively by precipitating and washing, and the yield of benzil products still remained as high as about 60% alter three cycles, which make this new green approach takes the advantages of more environmentally friendly, lower energy consumption and easier operation procedures compared to that of traditional method. [ABSTRACT FROM AUTHOR]

This paper discusses the experimental studies on the compatibility of drilling fluid lubricants with screw rubbers at high temperature and deep well conditions. At different temperatures and additive concentrations, eight kinds of rubbers were tested for rates of volume increment, rates of mass increment and changes in tensional strength when they were in contact with drilling fluid additives, and the test results were compared and evaluated. The experimental results show that the rubber sample has big changes in drilling fluids treated with the lubricants R-1 and R-3; in the drilling fluid treated with R-1, the rates of volume increment of the rubbers were distributed between 56% and 175%, while in the drilling fluid treated with R-3, the rates of volume increment were distributed between 49% and 148%. When the concentrations of the lubricants were increased, the rates of volume and mass increments of the two rubbers are all increased, and the rubbers swell at higher rates. These results indicate that the raw oil of a lubricant is the key factor affecting the swelling of rubber. Using GC-MS technology, the raw oils were analyzed, and it was found that a lubricant whose raw oil has high content of aromatics has relatively large influence on the swelling of the rubbers. Based on the application properties of the lubricants, some lubricants suitable for use in high temperature deep well drilling were selected. The selected lubricants were used to replace those lubricants with high contents of unsaturated hydrocarbons in the third interval and the reservoir section of the wells drilled in the Taipen block, and rubber disintegration from the screw was significantly reduced. Statistic data showed that times of rubber disintegration was reduced from 11 well times/month in the past to 0.5 well times/month, and this greatly reduced the number of times of tripping and improved the productive efficiency. [ABSTRACT FROM AUTHOR]

The experimet was to study the antioxidant activity of non-volatile components in Artemisia argyi leaves with different polarity solvent extracts and their antioxidant capacity, to investigate the relationship between antioxidant activity of non-volatile extract of Artemisia argyi leaf and content of polyphenols, flavonoids and polysaccharides. The lipid soluble pigment and volatile substances in Artemisia argyi leaves were removed by petroleum ether at boiling range of 60~ 90 ℃, the residues were extracted by ethyl acetate, n-butanol, ethanol and water, respectively. The antioxidant activities of four extracts were determined by ferric ion reduction method (FRAP) and 1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH) method. The antioxidant activities were converted into Trolox equivalent, determining the content of polyphenols, flavonoids and polysaccharides in extract. The results showed that in water phase, ethanol phase, n-butanol phase and ethyl acetate phase, polyphenols, flavonoids and polysaccharides were mainly enriched in water phase and ethanol phase. The content of flavonoids directly could affect the antioxidant activity of polyphenols. The study indicates that the antioxidant activity of non-volatile extracts of Artemisia argyi leaves mainly exists in aqueous and ethanol phases, and flavonoids are the main components of antioxidant activity, which can be further developed as natural antioxidant active components. [ABSTRACT FROM AUTHOR]

System biology, as an integrative medicine, elucidates the complex physiological and pathological states of organisms from the whole. It mainly includes genomics, proteomics, transcriptomic, metabolomics, and network pharmacology. Osteoporosis has the characteristics of high fracture risk, reduced bone strength, and deterioration of bone microstructure. Fragility fracture caused by osteoporosis is one of the main causes of disability and death in the elderly. The characteristics of overall analysis of systems biology have some commonalities with the characteristics of systemic metabolic diseases of osteoporosis to some extent. In recent years, the omics technology contained in system biology has been applied in the research work of osteoporosis, showing unique advantages. In this paper, the application progress of system biology technology in osteoporosis research in recent years is reviewed in order to provide a basis for the further application of system biology technology in osteoporosis research. [ABSTRACT FROM AUTHOR]

In order to solve the problems of easily fall into the local optimal value, low convergence accuracy and slow convergence speed in the basic slime mould algorithm（SMA）,this paper put forward an improved ISMA based on elite opposition-based learning and quadratic interpolation. Elite opposition-based learning strategy was conducive to improve the diversity and quality of population, improved the global optimization performance and convergence accuracy of the algorithm. It used quadratic interpolation to generate new individuals and fitness evaluation to update the global optimal solution was propitious to enhance the local development ability, reduced the convergence time and made the algorithm jump out of the local extremum. By solving multiple unimodal, multi-modal and high-dimensional test functions, the results show that the ISMA combined with the two strategies has higher optimization accuracy, optimization speed as well as robustness. [ABSTRACT FROM AUTHOR]

Objective The correlation between pulmonary artery diameter measured by high resolution computedtomography （HRCT） and pulmonary artery pressure measured by echocardiography in patients with chronic obstructive pulmonary disease （COPD） was investigated to find a new method to assess pulmonary artery pressure and to add a new method to the clinical assessment of the disease. Methods Seventy-seven patients with stable chronic obstructive pulmonary disease who were seen in Kunming First People’ s Hospital from May 2018 to May 2020 were set as the test group, and 50 healthy individuals who underwent health checkups in same period were selected as the control group, all of whom underwent chest HRCT to measure right pulmonary artery diameter, left pulmonary artery diameter, and main pulmonary artery diameter, and also underwent cardiac ultrasound to measure right ventricular diameter, pulmonary artery pressure, and main pulmonary artery diameter. The data collected were analyzed for correlation. Results The data of left and right pulmonary artery diameter and main pulmonary artery diameter measured by HRCT in the COPD group were larger than those in the normal group （P < 0.05）, and there were differences in the data of right pulmonary artery diameter and main pulmonary artery diameter between the COPD groups （P < 0.05）, but not in the left pulmonary artery diameter （P > 0.05）; the right intraventricular diameter and pulmonary artery pressure measured by echocardiography were different between the COPD groups （P < 0.05）. The right intraventricular diameter, pulmonary artery pressure, and main pulmonary artery diameter were significantly correlated with the left and right pulmonary artery diameters and main pulmonary artery diameter by Pearson linear correlation analysis （P < 0.05）, and all were positively correlated. Conclusio There is a significant correlation between the values of pulmonary artery pressure and the values of pulmonary artery diameter in patients with COPD. HRCT measurement of pulmonary artery trunk diameter and right pulmonary artery diameter can be used as a method to assess pulmonary artery pressure. [ABSTRACT FROM AUTHOR]

Objective To study the effect and clinical value of supervised quitting smoking and lung rehabilitation on patients with chronic obstructive pulmonary smoking. Methods The general data and pulmonary function indexes of male patients with chronic obstructive pulmonary disease （COPD） who had been hospitalized in our department from June 2018 to August 2019 were collected. According to the principle of order， they were randomly divided into control group and supervised group. The differences of general data， lung function indexes （1 week，half year） and smoking expenditure were compared. Results After six months of follow-up， the number of cigarrette [supervised group （11±11.995） and control group （17.1±11.784） ] decreased， and the difference was statistically significant （ ＜0.05） . Half a year later， compared with the two groups， the supervised group smoked much less than the control group， and the difference was statistically significant （ ＜0.05） . Estimated FEV1 （%） [supervised group （71.14±14.077%） and control group （65.20±14.028%） ] were different， with statistically significant difference （ ＜0.05） . Half a year later， compared with the two groups， the supervised group was superior to the control group， and the difference was statistically significant （ ＜0.05） . FEV 1 （supervised group supervised group 1.656 ±0.4708） L， control group （1.462 ±0.4637） L） showed no significant difference （ ＞0.05） . The control group decreased，and the difference was not statistically significant （ ＞0.05） . Half a year later， the difference between the two groups was statistically significant （ < 0.05） . FVC [supervised group （3.0144±0.5326） L， control group （2.9118±0.5048） L] in the supervised group increased， control group decreased，and the difference was not statistically significant （ ＞0.05） . Six months later， there was no significant difference between the two groups （ > 0.05） . Conclusion The supervision of quitting smoking and lung rehabilitation in patients with smoking COPD can reduce the number of cigarette， improve the success rate of quitting smoking， delay and even improve the pulmonary function of some patients， and the clinical effect is remarkable. [ABSTRACT FROM AUTHOR]

Objective: To investigate the phenotypic differences among homologous tumor clones derived from a single cancer cell. Methods: Using limited dilution method, the HCTI 16 colon cancer cells from the suspended culture condition were dissociated into single-cell suspension and seeded in ultra low attachment plates with average one cell per well. Within several weeks, the progeny clones derived from a single cell were obtained and expanded. By the same limited dilution method, the second and third generation of sub-monoclones, derived from one of their ex-generation clones respectively, were also acquired and expanded. Then, we chose the three representative clones of the third generation based on their distinct morphology for the further experiment The expression of SOX2, EpCAM and Vimentin were detected via western blot or immunofluorescence. Through X -ray irradiation, the temporal heterogeneity of the three representative clones was evaluated by their dynamic alteration of Vimentin expression before and after treatment The invasive ability of the three clones was also compared by transwell assay. Results: The considerable diversity of biological phenotype (eg: morphology; protein expression; invasive ability) was strll present among the three clones, even they were the homologous progeny oftlnrd generation derived from a single HCTI 16 cancer cell. Their morphology was obvious differences (irregular or well-rounded shape). The protein expression of the irregular clone (expression of SOX2 low and Vimentin high) was also different from other clones. Furthermore, the distinct protein expression was present at the single-cellular level (Vimentin; EpCAM), even they were among the same clone expanded from a single cancer cell. When the three homologous clones were treated with radiation, the temporal heterogeneity of Vimentin protein expression was present The distinct invasive ability was also observed among the three clones. Conclusions: There was still obvious biological heterogeneity among the third-generation clones, even they derived from consecutive single-cell expansion. It provided the evidence to consider that the heterogeneity is the tumor inherent characteristics, and the influence of treatment intervention will also induce the temporal heterogeneity in the tumor cells [ABSTRACT FROM AUTHOR]

Objective To study the efficacy and safety of bronchoalveolar lavage in the treatment of patients with severe CAP．Methods 76 patients with severe CAP were selected fromAugust，2016 to November，2018 in Ganmei Hospital Affiliated to Kunming Medical University(The First People's Hospital of Kunming) .All patients were randomly divided into 2 groups:treatment group (n = 38) and control group (n = 38) .The treatment group was treated with bronchoscopy and alveolar lavage. After 7 days， body temperature fluctuation， white blood cell， blood gas analysis (PaO2 and PaCO2) and total hospitalization time were observed.At the same time，we observedwhether there were serious adverse events during hospitalization in the two groups．Results After treatment， body temperature[treatment group(37.082±0.7822)℃，control group (37.603±0.7779) ℃]， white blood cell[treatment group (9.058±1.8232) ×109，control group (11.41±.0599) ×109] and PaCO2[treatment group (42.84±6.092) mmHg，control group (48.63±6.162) mmHg] were decreased in the two groups，and the difference was statistically significant(P<0.05) .At the same time， the effect of bronchofiberscope lavage in the treatment group was more significant， and the decrease was more obvious， the difference was statistically significant (P<0.05) .After treatment， PaO2 [treatment group(82.12±8.867) mmHg，control group (67.87± 9.154) mmHg ]increased， and the difference was statistically significant compared with that before treatment( < 0.05) .At the same time， the curative effect of bronchoscopy lavage in the treatment group was better and the increase was more significant than the control group，the difference was statistically significant(P<0.05) .Hospitalization time [treatment group (13.97±3.537) d，control group (20.19±3.763) d]of patients in the treatment group was shorter than that in the control gorup， the difference was statistically significant(P<0.05) ．Conclusion Bronchoscope alveolar lavage has significant clinical effect and no serious adverse reactions in the treatment of patients with severe CAP. [ABSTRACT FROM AUTHOR]

In order to develop a safe and efficient antibacterial material, the PHMB antibacterial film was prepared by preparing a polyhexamethylene biguanide hydrochloride (PHMB) antibacterial masterbatch and adding the antibacterial masterbatch to a polyethylene substrate. The effects of the amount of PHMB masterbatch (0~7%) on the antibacterial and physical properties of the film were compared, and the antibacterial film preservation experiment was carried out. The results showed that the antibacterial property of the film increased with the increase of the amount of PHMB masterbatch added. The antibacterial film has good physical properties and preservation effect. In the inhibition of Escherichia coli, when the amount of PHMB masterbatch added amount was 3%, the number of plaques after bacterial suspension was significantly reduced after contact with the film for 24 hours, and the antibacterial rate was 85.2%. When the amount of PHMB masterbatch addition was 4%, only 3 colonies eluted from the film, and the antibacterial rate was 99.1%. Moreover, the antibacterial rate data has a small variance, and the antibacterial film has a relatively stable and strong antibacterial effect. In the inhibition of Staphylococcus aureus, when the amount of PHMB masterbatch addition was 2%, 3%, the plaque eluted from the antibacterial membrane was significantly reduced compared with the CK membrane, and the antibacterial rate was 60.3%, 72.6%. When the amount of PHMB masterbatch addition was 4%, only 2 colonies were cultured on the plate and the antibacterial rate increased to 99.2%. The antibacterial film had strong antibacterial activity against Staphylococcus aureus. The antibacterial rate of PHMB (4%) antibacterial film against Shanghai green-loaded colonies was 83.4%, and the antibacterial rate of PHMB (6%) antibacterial film against Shanghai Qing loaded colonies was more than 90%. Shanghai Qing does not have any treatment, and various microorganisms in the field accompany it. The microbial species in the flora are diversified, and the antibacterial effect of the PHMB antibacterial membrane is still significant. This shows that PHMB antibacterial film has the characteristics of broad-spectrum antibacterial and strong antibacterial. After 6 months of storage of PHMB (4%) antibacterial film, the antibacterial rates against Escherichia coli and Staphylococcus aureus were 97.5% and 96.9%, respectively. The antibacterial rate of the film decreased slightly, but it still maintained strong antibacterial properties. It shows that PHMB antibacterial film has antibacterial long-term effect. After the addition of the PHMB masterbatch, the transmittance of the film began to increase gradually, followed by a slight decrease. At 560 nm wavelength, the transmittance of the control film was 86.9%, and the transmittance of the antibacterial film of PHMB (5%) was the highest, reaching 89.1%. In general, the transmittance of the antibacterial film did not change much and was not affected at all. After the addition of PHMB masterbatch, the water vapor transmission rate of the film showed an increasing trend, but the increase was not obvious. As the amount of PHMB masterbatch addition increased from 0 to 3%, the water vapor transmission rate of the antibacterial film increased from 28.86 to 35.25 g/(m2·d), and then tended to be steady. After the addition of the PHMB masterbatch, the moisture permeability of the film does not change much, and the moisture permeability is still maintained, which is beneficial to the storage and preservation of the food. With the increase of the amount of PHMB masterbatch added, the mechanical properties of the antibacterial film increased first and then decreased. The mechanical strength is the highest when the amount of the masterbatch is 4%, and the mechanical properties of the antibacterial film which added PHMB masterbatch is superior to those of the CK film. The PHMB (4%) antibacterial membrane is effective in inhibiting the growth of total colonies, Escherichia coli and Staphylococcus aureus in dried salmon samples. Samples packaged in PHMB antibacterial film can reduce the irradiation dose and save energy in the subsequent irradiation sterilization process. Therefore, it is determined that the optimal amount of PHMB masterbatch in the antibacterial film is 4%. The film has good processability, mechanical strength, and long-lasting antibacterial properties, while achieving non-toxic, odor-free, environmentally friendly requirements. The subsequent application of PHMB antibacterial film in various food storage and preservation can be further explored. This study provides a reference for the development of antimicrobial materials. [ABSTRACT FROM AUTHOR]

OBJECTIVE: To investigate the application of antibiotics in Traditional Chinese Medicine Hospital of Yangjiang (hereinafter referred to as “our hospital”), so as to provide reference for improvement of rational drug application. METHODS: Excel software was used, statistical analysis was conducted on consumption sum, defined daily dose system (DDDs), defined daily cost (DDC) and B/ A of antibiotics in our hospital during 2015-2017. RESULTS: During 2015-2017, the ratio of consumption sum of antibiotics to the total consumption sum of drugs (12%-14%) met the relevant requirements, and the overall trend was downward. The top 5 categories of antibiotics ranked by ranking of consumption sum and DDDs were respectively cephalosporins, penicillins and fluoroquinolones, among which the ranking of consumption sum and DDDs of cephalosporins took the lead. CONCLUSIONS: The distribution of antibiotics in our hospital and the application of most antibiotics are basically reasonable, in line with the requirements of the Guiding Principles for Clinical Application of Antimicrobial Drugs (2015 Edition). Yet there are still some problems. It is necessary to further strengthen the rational application of antibiotics, optimize the structure and promote the continuous improvement of the clinical application of antibiotics. [ABSTRACT FROM AUTHOR]

[Objective] This study aimed to discuss the important effect of thinning on the artificial forest. [Method] Taken four transformed stands (CK:0%, Ml:30% , M2:70% , M3:100%) of 12-year-old Eucalyptus urophylla plantations in the Dongguan Forest Research Park, Guangdong as study objects, the effects of thinning on the physical and chemical characteristics,microorganism and enzyme activities of soils in winter and summer 7 years after thinning were studied in this paper. [Result] (i) Thinning had some effects on soil physical and chemical properties between seasonal variations. In winter, the thinning treatment could increase soil conductivity, NH4-N, available K, exchangeable Mg and Na content, total N significantly reduced in Ml stand, and exchangeable Ca significantly reduced in Ml and M3 stands; In summer, thinning increased soil total N, NH4-N, N03-N and total P but decreased exchangeable Ca. (ii)There were significant differences in soil microorganism between seasonal variations, and thinning had significant effects on soil microbial biomass C and N, and the soil microorganism in summer was significantly higher than that in winter. The soil microbial biomass C and N showed a trend of increasing first and then decreasing in winter and summer with the increase of thinning intensity. (iii) thinning had no significant effects on soil enzyme but could increase phosphatase and urease activities; The phosphatase activity was higher in summer and lower in winter; However, the urease activities were opposite, (iv) PCA indicated that the soil factors responses the thinning and unthinned stands in winter and summer. Thinning stand was scattered in winter, while the M2 and M3 stand in summer were sorted together, which related to the soil pH, NH4-N, N03-N and catalase activity. [Conclusion] The soil physical and chemical properties would closely related to the balance and sustainable development of soil ecosystem after thinning of Eucalyptus urophylla plantation. [ABSTRACT FROM AUTHOR]