1. Immunogenicity of a vaccinia virus-based severe acute respiratory syndrome coronavirus 2 vaccine candidate
- Author
-
Shan, Mei, Zhangling, Fan, Xiaoman, Liu, Fei, Zhao, Yu, Huang, Liang, Wei, Yamei, Hu, Yu, Xie, Liming, Wang, Bin, Ai, Chen, Liang, Fengwen, Xu, and Fei, Guo
- Subjects
Mice ,COVID-19 Vaccines ,SARS-CoV-2 ,Spike Glycoprotein, Coronavirus ,Immunology ,Animals ,COVID-19 ,Humans ,Immunology and Allergy ,Vaccinia virus ,Viral Vaccines ,Antibodies, Viral ,Pandemics - Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines provide essential tools for the control of the COVID-19 pandemic. A number of technologies have been employed to develop SARS-CoV-2 vaccines, including the inactivated SARS-CoV-2 particles, mRNA to express viral spike protein, recombinant spike proteins, and viral vectors. Here, we report the use of the vaccinia virus Tiantan strain as a vector to express the SARS-CoV-2 spike protein. When it was used to inoculate mice, robust SARS-CoV-2 spike protein-specific antibody response and T-cell response were detected. Sera from the vaccinated mice showed strong neutralizing activity against the ancestral Wuhan SARS-CoV-2, the variants of concern (VOCs) B.1.351, B.1.617.2, and the emerging B.1.1.529 (omicron). This finding supports the possibility of developing a new type of SARS-CoV-2 vaccine using the vaccinia virus vector.
- Published
- 2022