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In order to improve the accuracy of respiratory signal to distinguish driving fatigue, the relationship was explored between respiratory signal and driver fatigue state through simulated driving test. The concept of respiratory fatigue node was proposed, and the driver's fatigue state was distinguished based on the respiratory fatigue node. Firstly, the respiratory signals of drivers were collected by simulated driving test, and the Karolinska sleepiness scale (KSS) was used to quantify the subjective self-evaluation of their fatigue degree. Secondly, the percentage of eyelid closure over the pupil over time (PERCLOS) was used as a reference, combined with subjective self-evaluation feedback to calibrate the respiratory fatigue nodes of drivers. Finally, based on the respiratory fatigue nodes, the random tree(RT) algorithm was used to obtain the discriminant model of mild / severe respiratory fatigue change nodes. The results show that the model can identify the driver's respiratory fatigue state more timely and accurately and the accuracy of the screening conditions based on the random tree algorithm for the identification of mild respiratory fatigue change nodes is higher than that of severe respiratory fatigue change nodes. The average recognition errors of mild / severe respiratory fatigue change nodes are 3. 50 min and 3. 66 min, respectively, the prediction accuracy is 92. 09% and 92. 03% respectively. [ABSTRACT FROM AUTHOR]

To address the challenges posed by the poor sensitivity of traditional degradation indices to the onset of bearing degradation and the inability to accurately predict the remaining using life (RUL) of wind turbine bearings due to trend shifts in degradation indices, a biexponential method for predicting bearing RUL was proposed. This method was based on the combination of Teager's energy arithmetic-ratio of failures to energy (TFER) and degradation trend (DT) similarity detection. Firstly, the TFER value of the original signal was calculated to determine the bearing degradation starting point following the 4σ principle. The trend feature of the TFER value was then extracted as a degradation indicator. Subsequently, a bi-exponential degradation model was fitted using historical TFER values. Then, the best DT segment was chosen by analyzing the similarity between the latest TFER values and the fitted curve. Finally, the wind turbine bearing RUL was predicted by extrapolating the DT segment with the best similarity to the failure threshold. The experimental results show that the prediction method achieves 98% accuracy in detecting the degradation onset time point, and improves 10. 04%, 6. 29%, and 5. 22% in bearing RUL prediction accuracy compared with the original exponential model, the long-and short-term memory neural network (LSTM), and the support vector regression (SVR), respectively. This method not only improves the accuracy of predictive maintenance of wind turbine bearings, but also provides strong support for reducing operating costs and improving maintenance efficiency. [ABSTRACT FROM AUTHOR]

African swine fever virus(ASFV)possesses multiple strategies for escaping host immune responses, making it difficult for the virus to be cleared by the host. The cGAS-STING pathway, which mediates host innate immune response, is critical for fighting ASFV infection. Nevertheless, numerous viral proteins targeting different molecules involving in the cGAS-STING pathway inhibit type I interferon production. The deletion of partial of these ASFV genes using genome editing technology attenuated the virulence of the virus, which provides targets for the development of gene deletion vaccine. In this review, we summarized current knowledge on the viral proteins of ASFV modulating host cGAS-STING signaling pathway and elucidate the mechanism of these proteins escaping cGAS-STING signaling pathway, which may deepen the understanding of ASFV immune evasion strategy and provide theoretical basis for the research of ASFV pathogenic mechanism and vaccine development. [ABSTRACT FROM AUTHOR]

In response to the copper cyanide barren solution generated by a gold production company, a joint recovery treatment using complex precipitation and sulfide precipitation was adopted. Under the conditions of adding 3.0 g/L of copper sulfate pentahydrate and 2.0 g/L of sodium metabisulfite in the complex precipitation process, and adding 0.35 g/L of sodium sulfide nonahydrate in the sulfide precipitation process, the mass concentrations of total cyanide compounds, copper, and thiocyanate in the copper cyanide barren solution decreased from 150.84 mg/L, 121.46 mg/L, 252.65 mg/L to 0.44 mg/L, 86.17 mg/L, 1.23 mg/L, respectively. The production value of gold, silver, and copper recovery was 116.62 yuan/m³, generating an economic benefit of 55.44 yuan/m³ after deducting the cost of reagents. The research results provide a reference for the purification treatment of similar cyanide barren solutions in cyanide enterprises. [ABSTRACT FROM AUTHOR]

In the process of silo discharge, the flow pattern of storage material changes constantly, and the pressure of silo wall shows different change rules. To investigate the flow pattern and velocity distribution, dynamic lateral pressure and corresponding overpressure coefficient during the discharge process, discrete element simulation software engineering discrete element method (EDEM) was used to simulate under two different discharge speed conditions, and the dynamic lateral pressure was compared and analyzed combined with the test results. The results show that the flow pattern of the storage material is related to its position by direct observation and overall flow index analysis, and the development process is integrated flow, tubular flow and funnel flow. The EDEM simulation results show that the static lateral pressure value and the dynamic lateral pressure distribution law are consistent with the test results, and the overpressure coefficient error is within a reasonable range. In the whole process of discharge, the overpressure coefficient under the fast velocity condition is greater than that under the slow velocity condition. The dynamic lateral pressure surge is the most obvious in the lower 1/3hn of the silo wall(hn is the height of the silo loading line to the bottom of the silo wall), and the repeated dynamic arch process inside the silo results in different vibration distribution of discharge pressure on the wall, and the overpressure coefficient at this location is higher than the current standard correction coefficient of horizontal lateral pressure Ch. It is feasible to study dynamic lateral pressure by EDEM, and the research results can provide reference for the safety design of silo structures. [ABSTRACT FROM AUTHOR]

In order to improve the ability of remote monitoring and intelligent prevention and control of the operating environment and public safety status of intelligent substation equipment, a substation intelligent auxiliary control system based on three-dimensional model was proposed. According to the equipment selection and layout requirements of substation intelligent auxiliary control system, the database was developed and designed. The 3D model was used to share the 3D design results of substation intelligent auxiliary control system. The 3D design of substation intelligent auxiliary control system was completed through 3D design model verification and infrared dual-authentication 3D modeling, and the 3D modeling algorithm was optimized to obtain the 3D modeling output results. The simulation results show that the system has good output power stability, strong anti-interference ability, high efficiency of closed-loop management and processing of operation and maintenance, which is conducive to improving the remote monitoring and intelligent prevention and control ability of substation equipment. [ABSTRACT FROM AUTHOR]

[Objective] To solve the problem of difficult valuation of agricultural scientific and technological achievements, a method for dynamically evaluating the transaction price of agricultural scientific and technological achievements using computers was studied, providing data support and pricing basis for the transformation, transaction or transfer of agricultural scientific and technological achievements. [Method] A five-layer Fuzzy neural network model FNN (Fuzzy neural network) was constructed by combining fuzzy theory and neural network, which Abstract: [Objective] To solve the problem of difficult valuation of agricultural scientific and technological achievements, a method for dynamically evaluating the transaction price of agricultural scientific and technological achievements using computers was studied, providing dalearned and stored rule knowledge from the historical transactions data of agricultural scientific and technological achievements and used it to predict the transaction price of the achievements. The accuracy rate of FNN was only 80% after the actual business data test. In order to improve the evaluation accuracy, adapt to the changes caused by increasing transaction samples, reduce the resource consumption of retraining all sample data, so introduce incremental learning, an improved fuzzy neural network model IFNN (Improved fuzzy neural network) and an improved valuation process were proposed. [Result] In the actual business data processing, by applying the improved valuation process and completing the training of incremental data, the IFNN's valuation accuracy rate reached 86.7%. Fifteen rapeseed varieties were selected to compare the results of FNN and IFNN model. The fuzzy membership degrees of FNN and IFNN were different, and the comparison showed that the accuracy of IFNN was higher than FNN. The IFNN model algorithmically met the requirement that the estimation accuracy increased with incremental learning. However, in order to further improve the accuracy of the estimation, more complete training data was needed. [Conclusion] The IFNN can dynamically estimate agricultural scientific and technological achievements based on objective data in practical business, and can adapt to the constantly changing and developing society, and has high practical application value. [ABSTRACT FROM AUTHOR]

Belt and Road countries and regions have increasingly recognized the urgent need for climate mitigation and adaptation in order to achieve the Paris Agreement and the Sustainable Development Goals (SDGs). Transferring and disseminating climate technologies are effective ways in which developing countries in the region can enhance their capacity to address the challenges of climate change. This study assesses the characteristics of climate change technology needed in Belt and Road countries and regions, based on the United Nations Framework Convention on Climate Change's Technology Needs Assessment (TNA) report as well as the Cooperative Patent Classification (CPC) system. It identifies and analyzes the priority needs for mitigation and adaptation technology needs in different areas. Results shows that climate technologies are in high demand in the Belt and Road region. A total of 726 climate technologies are included in the TNAs of 66 Belt and Road countries and regions, of which 262 are mitigation technologies and 464 are adaptation technologies. Almost all countries prioritize renewable energy for electricity generation (Y02E-10) for mitigation, with 135 technologies listed, covering Gross Domestic Production (GDP) of $5.3 trillion and a population of 1.9 billion. The most urgent need for adaptation technology is in agriculture, forestry, livestock, and fisheries production (Y02A-40), with 48 countries claiming 234 technologies for adaptation across this sector, covering $3.8 trillion in GDP and 1.61 billion in population. There is a high degree of heterogeneity in mitigation technology demands across regions. African countries are most in need of renewable energy generation technologies (Y02E-10), non-fossil fuel technologies (Y02E-50), and mitigation technologies for the land sector (Y02A-60). Asian countries are most interested in renewable energy generation and combustion technologies (Y02E-20), while Latin American and Oceania are most interested in mitigation technologies for road transport (Y02T-10). There is a high degree of commonality in the adaptation technology needs of all regions, with the priority needs of each region being primarily for adaptation technologies in agriculture, forestry, livestock and fishery production (Y02A-40) and technologies for harvesting, conserving, and efficiently using water (Y02A-20). In order to promote South-South technology cooperation between China and BRI developing countries, the proposed suggestions include: (1) better matching the technology supply by China with the demands in BRI countries and regions; (2) enhancing the transparency of technology information in China through various measures; (3) strengthening country-specific studies on climate technology needs and transfer strategy for BRI countries. [ABSTRACT FROM AUTHOR]

Objective The supply and sales difference has always been a key point in the management and control of LNG filling stations. Reducing the supply and sales difference of LNG filling stations, especially inefficient LNG filling stations, is of great significance for cost control. Methods According to the actual production and operation status of the station, the specific reasons for the supply and sales difference of LNG filling stations are comprehensively analyzed from the aspects of sales measurement, trade procurement, unloading process and storage. For some factors that enlarging supply and sales difference, the targeted control measures shall be taken from the aspects of gas source procurement, process pipeline design, thermal insulation construction, submersible pump installation, tank release control and station construction mode, etc. Results Through the measurement of practical data after the process transformation and the refined management of production and operation, the supply and sales difference of LNG filling stations has been effectively reduced. Conclusions In the initial design stage and actual operation and management stage of LNG filling stations, we should confirm the station construction standard and strengthen the management and control of LNG filling stations to reduce supply and sales difference according to characteristics of LNG properties, so as to improve the profit of LNG filling stations. [ABSTRACT FROM AUTHOR]

脊髓损伤 (SCI) 导致患者运动和感觉功能丧失, 至今仍缺乏理想的治疗方法。干细胞具有神经再生和神 经保护特性, 干细胞移植有望成为治疗 SCI 的有效方法。但是, 干细胞治疗 SCI 需要趋化因子介导其定向迁移至损 伤组织部位, 基质细胞衍生因子 1α（SDF-1α）则在其中起重要作用。SDF-1α/CXCR4 轴在 SCI 干细胞治疗中发挥重 要作用, 它既对多种干细胞的迁移、增殖和分化起调控作用, 又能促进轴突生长和重新髓鞘形成, 进而改善 SCI。 [ABSTRACT FROM AUTHOR]

BACKGROUND: With the increase of population, urbanization development, the large discharge of industrial wastewater and the excessive application of agricultural nitrogen fertilizer, the problem of “three nitrogen” (nitrate nitrogen, ammonia nitrogen, and nitrite nitrogen) pollution in groundwater has become increasingly serious. Due to the slow flow, weak alternation degree and poor self-purification ability, the nitrogen polluted groundwater is difficult to rehabilitate and the repair cost is high. It is of great practical significance to carry out prevention and control from the pollution source. With the concealment, complexity and hysteretic groundwater pollution property, it is difficult to conduct the source analysis of nitrate in groundwater. Positive matrix factorization (PMF) model, as a new type of source analysis model, has more interpretable and clear physical significance in terms of factor load and source factor score. At present, the application of the PMF model in the source analysis of “three nitrogen” in groundwater media is rarely reported, and the research in this field needs to be deepened. Gas-phase molecular absorption spectrometry is a practical analytical method for nitrogen compounds with high accuracy, wide linear range without color and turbidity interference. This method does not require chemical separation, and uses few chemical reagents and innoxious reagents. At present, this method has been widely used in the analysis and determination of water quality, nitrite nitrogen, ammonia nitrogen, nitrate nitrogen, total nitrogen, and other substances. OBJECTIVES: To identify the source and distribution characteristics of the “three nitrogen” in shallow groundwater in the Hailun area. METHODS: (1) Determination of “three nitrogen” and other elements in groundwater samples. With GPS coordinates, the 40 shallow groundwater samples were collected in Helen River Basin and Zaying River Basin. The collection and preservation of groundwater samples were performed in accordance with the environmental standards HJ/T 164—2004, HJ/T 195—2005, HJ/T 197—2005 and the ecological industry standards DZ/T 0064—2021. The quality control of the “three nitrogen” test in groundwater was based on the requirements of the national standard GB/T 14848—2017, the technical requirements for analytical quality control of groundwater pollution investigation (DD 2014-15), and the specification of testing quality management for geological laboratories (DZ/T 0130—2006). Each batch contained twenty samples and the test process of each batch was equipped with laboratory blank, reference materials, laboratory duplicate samples and quality control of external monitoring samples. All of the laboratory blank results were less than 2 times the detection limit of the method. The added standard recovery rate of the sample matrix was between 80%-120%, with the relative deviation (RD) of the laboratory repeat samples under 15%. The qualified rate of external quality control sample was 100%. (2) Analysis of detection data. Statistics and analysis of “three nitrogen”, heavy metal content correlation and principal components in groundwater were carried out using WPS Office 2016 and SPSS 22.0. The spatial distribution map of “three nitrogen” content in groundwater was obtained by CoreDRAW X5.0 software. The Ⅲ class water criterion in Standard for Groundwater Quality (GB/T 14848—2017) was used as the criterion for drinking water source and industrial and agricultural water. The Nemerow pollution index was calculated and compared with the corresponding grade standard index. The PMF model was used to identify and quantify the contribution rate of each nitrogen source to comprehensively judge the source of “three nitrogen”. RESULTS: (1) The contents of “three nitrogen” and other elements in the groundwater samples in the study area were determined. According to the contents of “three nitrogen” and related parameters shown in Table 3, the detection rates of the nitrate nitrogen, ammonia nitrogen and nitrite nitrogen were 100%, 77.5% and 90.0%, respectively. The maximum detection content of nitrate nitrogen was 123.05mg/L, and average content was 15.27mg/L. The maximum detection content of ammonia nitrogen was 3.91mg/L, and average content was 0.33mg/L. The maximum detection content of nitrite nitrogen was 0.65mg/L, and average content was 0.12mg/L. Compared with the Ⅲ class water criterion, the nitrate nitrogen over standard rate was 20%, with the maximum value 6.18 times; the ammonia nitrogen over standard rate was 12.5%, with its maximum value 7.8 times; the nitrite nitrogen did not exceed the Ⅲ class water criterion. The content of Mn was ND-3.20mg/L, and the proportion of Mn in I-III class water range was 40%. The contents of 8 heavy metals including Cd were within 10 times the detection limit, with 100% Ⅰ-Ⅲ class water. The content of anion F− was 0.029-0.69mg/L. The content of anion Cl− ranged from 0.0027 to 310mg/L, with the 97.5% Ⅰ-Ⅲ class and 2.5% over standard rate. The content of SO4 2− was 0.46- 433mg/L, with the 95% Ⅰ-Ⅲ class and 5% over standard rate 　(2) The pollution and spatial distribution of groundwater were studied. The results of Nemerow comprehensive pollution evaluation showed that the pollution degree of “three nitrogen” in the study area was relatively low. The proportion of unpolluted samples, mild pollution samples, heavy pollution samples and serious pollution samples was 65%, 25%, 2.5% and 0, respectively (Table 4). “Three nitrogen” spatial distribution is shown in Fig.1a-c. Samples with ammonia nitrogen content ranging in Ⅰ-Ⅲ class water was mainly concentrated in the north, west, east and central; the Ⅳ class water samples were concentrated in the middle; the class V water samples were concentrated in the center. Fig.1a shows the trend of high ammonia nitrogen in the middle and low ammonia nitrogen on both sides. In Fig.1b, Ⅰ-Ⅲ class water samples of nitrate nitrogen were mainly concentrated in the central, east and north, with the Ⅳ class water in the middle and the class V water in the west. The samples with the highest and sub highest nitrate nitrogen content were located in the center of the study area, radially distributing along the Helen River Basin and the Zaying River Basin. The nitrate nitrogen content was high near the central area, with the low content far from the central area. The nitrite nitrogen in Fig.1c was Ⅰ-Ⅲ class water. The spatial distribution of the nitrite nitrogen shows the trend of high in the north and low in the south along the Helen River Basin. (3) Multivariate statistical analysis and source of “three nitrogen” in groundwater were studied. Multivariate statistical analysis of Pearson correlation coefficient showed that nitrate nitrogen had some homology with Cd, Co, Ni. Nitrate nitrogen in groundwater was significantly correlated with Cl− and SO4 2−, while ammonia nitrogen and nitrite nitrogen showed no correlation with anion. Sample detection data imported into EPA PMF5.0 software for PMF source analysis, and the industrial source, agricultural source, living source and natural factor source were taken as the four source analysis factors of “three nitrogen” in the groundwater in the study area. The PMF source analysis results are presented in Table 6 and Fig.2. The main sources of “three nitrogen” in the groundwater in the study area were the compound source of living and industrial source, which further indicated that human activity was the root cause of “three nitrogen” pollution in the groundwater in Helen area. According to the spatial distribution of “three nitrogen” in groundwater, the highest point of nitrate nitrogen pollution was distributed in the center of the research area, with the relatively developed human life and industrial activities in this area. The ammonia nitrogen pollution samples were related to the dense human activities in the middle of the study area. The over-standard area of nitrate nitrogen was higher than the ammonia nitrogen, which indicated that the compound source of living and industrial source was the main factor affecting the “three nitrogen” content in the groundwater in study area. CONCLUSIONS: The nitrate nitrogen and ammonia nitrogen are the nitrogen pollution components in the groundwater in the study area. Compared with the water index in groundwater quality standard (GB/T 14848—2017), the over standard rates of the nitrate nitrogen and ammonia nitrogen are 20% and 12.5% respectively. The groundwater quality with pollution evaluation grade I (unpolluted) to grade Ⅲ (moderate polluted) accounts for 92.5%, which indicates relatively light pollution. The spatial distribution of ammonia nitrogen in the study area shows a trend of high in the middle and low on both sides. Nitrate nitrogen and ammonia nitrogen pollution samples are mainly distributed in the center of the intensive human activity research area. The quantitative analysis results of the “three nitrogen” PMF analysis model show that the compound source of living and industry are the main source of nitrate nitrogen pollution in the study area, and the compound source of living and agricultural production is the main source of ammonia nitrogen pollution. Combining the situation with the source of the “three nitrogen” pollution in the research area, the project of the “three nitrogen” pollution prevention can be carried out to strengthen protection, sustainable development and utilization of groundwater resources in the Helen area. [ABSTRACT FROM AUTHOR]

Objective For the gasification station or gas filling station that uses LNG as the gas source, due to the current LNG unloading process, after the unloading of LNG tanker, there is still a certain amount of paid but unusable BOG in storage tank. In view of this common problem, an effective method for recovering BOG is studied. Methods Taking a gasification station as an example, by calculating the recoverable BOG amount in the LNG tanker storage tank, combined with the recovered BOG state parameters, the unloading and operation process flow of the gasification station, the corresponding process transformation scheme is formulated. Results The operation conditions of the main equipment to be selected are simulated. The state diagram of the exhaust capacity and power changing of the single-stage and two-stage compressors with time, and the state diagram of the BOG pressure and margin changing in the tanker with time are drawn. The efficiency of the equipment to be selected is compared visually. Conclusions In the transformation scheme of LNG tanker BOG recovering process in the gasification station, the selection of two-stage low-temperature compressor can reduce the emission and the unloading loss of tanker effectively, which has more advantages. [ABSTRACT FROM AUTHOR]

Objective To explore the expression of miR-101-3p in gastric cancer and its mechanism on the invasion, metastasis, and angiogenesis of gastric cancer cells by targeting the STC-1 gene to regulate the PI3K/AKT signaling pathway. Methods qRT-PCR was used to detect the expression of miR-101-3p and STC-1 mRNA in gastric cancer tissues and BGC-823 cell and analyze the relationship between miR-101- 3p expression and patients’ clinical pathological factors. The cells were transfected with miRNA mimics and plasmids separately or in combination with LipofectamineTM 2000. TargetScanHuman prediction and dualluciferase assay were used to verify the targeted regulation of miR-101-3p on STC-1. The effect and possible mechanism of miR-101-3p targeting the STC-1 gene on the invasion, metastasis, and angiogenesis of cancer cells were verified by scratch test, Transwell chamber test, Matrigel in vitro tube forming test, and Western blot assay. The development of the transplanted tumor was detected by nude mouse tumorigenicity test. Results The expression of STC-1 in gastric cancer tissues was higher than that in normal tissues. Compared with normal gastric tissues and GES-1 cells, miR-101-3p was down-regulated, and STC-1 mRNA was upregulated in gastric cancer tissues and BGC-823 cell. The level of miR-101-3p was negatively correlated with the level of STC-1, and significantly correlated with the degree of tumor differentiation, TNM stage, and lymph node metastasis (P<0.05). miR-101-3p directly targeted STC-1. The overexpression of miR-101- 3p inhibited STC-1 expression and downregulated the expression of p-PI3K/PI3K, p-AKT/AKT, MMP-2, MMP-9, VEGF, and Ang2, consequently, inhibited tumor cell invasion, metastasis, and angiogenesis and reduced the size and weight of the transplanted tumors (P<0.05). Conclusion miR-101-3p is down-regulated in gastric cancer and can target the STC-1 gene to regulate the PI3K/AKT signaling pathway and inhibit the invasion, metastasis, and angiogenesis of BGC-823 gastric cancer cells and the development of transplanted tumors in vivo. [ABSTRACT FROM AUTHOR]

【Objective】The study aimed to understand the temporal and spatial distribution of planktonic viruses and abnormal bacteria between different functional divisions in Zhoushan sea area. 【Method】In the winter of 2017, spring of 2018, summer and autumn, six typical stations were selected in different functional areas of the coastal waters of Zhoushan island to collect surface seawater and obtain 24 samples. Flow cytometry was used to detect the abundance of planktonic viruses and abnormal bacteria, and the related environmental factors and their temporal and spatial distribution characteristics were analyzed. 【Result】The seasonal distribution of the Virus（Viruses, Vir） was: Summer>autumn>winter>spring, and the seasonal distribution of Heterotrophic Bacteria（HB） was: Summer>spring>autumn>winter. Correlation analysis showed that there was a significantly positive correlation between planktonic virus and abnormal bacteria and pH in spring, and a significant negative correlation with COD. There was a significantly positive correlation between planktonic virus and VBR. In summer, the abnormal bacteria showed a positive correlation with Chl-a. COD showed a significant negative correlation; In autumn, the floating virus was positively correlated with Chl-a and negatively correlated with COD. The abnormal bacteria were positively correlated with pH and negatively correlated with SiO32- and COD. In winter, planktonic virus and Chl-a positive correlation was observed, and the abnormal bacteria showed a positive correlation with pH and a significant negative correlation with COD. 【Conclusion】It was found that the abundance of planktonic viruses and abnormal bacteria in the coastal waters of Zhoushan can directly or indirectly reflect the pollution of the sea area, and can be used as one of the indicators for detecting water pollution. The above results provided valuable reference materials for the protection of the ecological environment in the different functional areas of Zhoushan, and provided basic information for a more comprehensive study of the ecological environment of the coastal waters of Zhoushan. [ABSTRACT FROM AUTHOR]

Breast cancer is a malignant tumor with the highest incidence in women in the world, and it represents the leading cause of cancer-related deaths in women. Breast cancer is a highly heterogeneous disease, including the heterogeneity between tumors and even within the tumor microenvironment, making precise classification and treatment of the disease difficult. The application of traditional high-throughput sequencing technology in breast cancer research has provided an important reference for revealing the occurrence and development of breast cancer, driving genes, precise classification, diagnosis and treatment. However, the limitation of traditional high-throughput sequencing technology is its inability to explore heterogeneity within the tumor microenvironment. The single-cell sequencing technology emerged in recent years has been greatly improved in terms of sensitivity, accuracy and efficiency, enabling us to analyze single-cell genome information in complex mixed cells, which has shown great performance in studying tumor heterogeneity. In recent years, there are a number of important research results obtained by harnessing single-cell sequencing technology in breast cancer research, including analyzing breast cancer transcriptome at the single-cell level, exploring the heterogeneity of breast cancer microenvironment, breast cancer drug resistance mechanisms, the clonal heterogeneity and clonal evolution of breast cancer, and comprehensively mining single-cell public data resources to reveal the heterogeneity of breast cancer. This article reviewed important research in recent years regarding the application of single-cell sequencing technology in breast cancer era. We aimed to provide references for breast cancer researchers to manipulate the single-cell sequencing technology and promote the in-depth use of single-cell sequencing technology in breast cancer research. [ABSTRACT FROM AUTHOR]

Objective: The ultra-small superparamagnetic iron oxide particles were used as the carrier to construct and characterize nuclear magnetic/fluorescent molecular probes through polyethylene glycol-linked fluorescent Cy5.5. Methods: Ake a small amount of Cy5.5-nhs fluorescent powder and dissolve it in dimethyl sulfoxide （DMSO） solution. After centrifugation and ultrafiltration of PEG ferric oxide particles, resuspend the nanoparticles with phosphate buffered saline （PBS） to change the pH of PEG ferric oxide nanoparticle solution. The configured cy5.5 fluorescence was added to Fe3O4particles, incubated in a constant temperature shaking table, and the larger iron ions and unbound fluorescence were removed through a centrifugal filter. After standing, the hydrated particle size and zeta potential were detected, the relaxation rate was detected by newwheat nuclear magnetic resonance, the cytotoxicity was detected by CCK-8 experiment, and the uptake of the probe by cells was observed by laser confocal microscope. Results: The Cy5.5-PEG-Fe3O4probe was successfully synthesized. Transmission electron microscope （TEM） showed that the diameter of the probe was 16.8 ±2.4nm,the water and diameter of the nanoparticles were 43.4±17.6 nm, and the Zeta potential was -18.0 m V. The relaxation rate is 39.5 m M-1·s-1and the slope is 0.98. The results of cytotoxicity experiments show that it is slightly toxic to cells, and the toxicity is dependent on the concentration. Laser confocal results show that this probe can be taken up by cells smoothly. Conclusion: This experiment successfully synthesized Cy5.5-PEG-Fe3O4probe. [ABSTRACT FROM AUTHOR]

Lithium residue is a solid waste generated in the production of lithium carbonate from zinnwaldite, typically rich in silicon dioxide, aluminum oxide, calcium oxide, and iron oxide. Moreover, lithium residue after calcination can produce a glassy phase, which is a potential precursor for preparing construction materials. By testing the physical and chemical property of lithium residue, experiments were designed to improve the filling ratio of lithium residue cement using an activator. The compressive strength of the filling body at 7 d and 28 d was tested, and the mixture formula was optimized using Design Expert software. The results show that the compressive strength of the optimized lithium residue filling body meets the filling requirements at 28 d, indicating its potential to substitute cement as underground filling and cementitious material, thus reducing filling costs and realizing the utilization of solid waste resources. [ABSTRACT FROM AUTHOR]

Granular grain can be stored into the warehouse in food industry. The high loading efficiency is often required to optimize the land use for the effective grain preservation. The crucial warehouse mode has emerged in the regions with limited land resources. The diverse grain varieties can be stored to promote the sustainable development. Currently, the granular grain warehouse normally employs a load-bearing system that constituted by a reinforced concrete frame structure. The vertical gravity load of the grain is supported by the well-beam floors and large-section frame columns, while the lateral pressure exerted by the grain is directly absorbed by reinforced concrete horizontal tie-up beams integrated within the warehouse walls. The structural integrity of granular grain warehouses can also depend on the dynamic lateral pressure exerted by the storage material on the warehouse wall during seismic events. In this study, a scaled-down 1:25 three-story warehouse was designed and then produced to explore the effect of granular grains on the dynamic lateral pressure. Shaking table tests were carried out to simulate the varying ground vibration levels under three seismic waves. The data was collected on the acceleration and the lateral pressure that exerted on its walls. Analysed the seismic response characteristics of the storage material. Furthermore, the overpressure coefficient was obtained from the patterns of dynamic lateral pressure on the walls. The dynamic lateral pressure on the warehouse walls was also calculated. Acorrelation analysis was conducted between the height of warehouse and the timing of the peak moment, when the dynamic lateral pressure was exerted on the warehouse walls. Specifically, there was a noticeable lag in the occurrence of this peak moment, as the height of the building increased. Moreover, the peak moment of dynamic lateral pressure on the warehouse wall was consistently lagged behind that of acceleration on the same wall, particularly with the elevation of the height. The dynamic lateral pressure on the warehouse walls escalated progressively with each ascending floor. In the given floor, this dynamic lateral pressure incrementally intensified along the vertical extent of the warehouse walls. Each floor was found with an average increase of approximately 29% per floor. The dynamic lateral pressure exerted on the upper and middle sections of the warehouse walls was 2.5 and 1.4 times greater than that on the lower sections within the same floor. Consequently, the structural design of granular grain warehouses should consider these variations across different floor levels. The overpressure coefficient of the warehouse wall increased with the elevation of the floor, indicating a higher overall overpressure at upper levels. Within a single floor, the overpressure coefficient peaks at the upper section of the warehouse wall, with the middle and lower sections exhibiting lower and closer. Specifically, the maximum overpressure coefficients were 2.9, 3.4, and 4.1, respectively, for the first, second, and third floors of the warehouse walls. The overpressure on the warehouse walls was of significant concern, when subjected to seismic activity. It is very necessary to consider the influence of the dynamic lateral pressure on the warehouse wall across various floors. The seismic response of the storage materials can be used to elucidate the distribution pattern of dynamic lateral pressure. The overpressure on the warehouse walls can be assessed to calculate the lateral pressure on these walls. The findings can offer a strong reference for the warehouses of granular grains under seismic conditions. [ABSTRACT FROM AUTHOR]

The pathological diagnostic criteria for early hepatocellular carcinoma( eHCC) have been updated in 2009, but the imaging diagnostic criteria for eHCC have not yet been established by domestic or foreign academic organizations. Promoted by Radiology of Infection Sub - branch, Radiology Branch, Chinese Medical Association, associating with Committee on Radiology of Infection, Radiology Branch, Chinese Medical Doctor Association; Radiology Committee on Infectious and Inflammatory Disease, Chinese Research Hospital Association; Radiology of Infection Branch, Working and Treating Committee of HIV/ AIDS and STD Association; Radiology of Infectious Disease Management Sub - branch, Infectious Disease Management Branch, Hospital Management Association in China; Beijing Imaging Diagnosis and Treatment Technology Innovation Alliance, this consensus was formed under the efforts of more than 40 experts from more than 10 domestic provinces and cities, so as to help related professional doctors in diagnosis of eHCC. [ABSTRACT FROM AUTHOR]

To investigate the effect of betaine ( TCJ ) on bone loss in type 2 diabetic rats and explore the possible mechanisms. Methods Thirty rats were randomly divided into a control group (CON), a model group (MOD), and a betaine group ( TCJ ), 10 in each group. Among them, type 2 diabetes models were established for MOD and TCJ group. Then TCJ group rats received 50 mg per day I kg betaine treatment for 12 weeks; after the treatment, they were detected by Micro-CT, HE stained sections and Western blot. Results After 12 weeks of treatment, compared with the MOD group, the result of the three-point bending test, Micro-CT and HE staining showed that the number of trabeculae, bone strength and bone density of the TCJ group were significantly improved(P<0.05).The maximum load and elastic modulus, BMD, TV/BV, Th.N, Tb.Th and Tb.Sp of the TCJ group were significantly improved compared with the MOD group ( P-catenin in the TCJ group was significantly increased, while the expression levels ofp-IGF-lR, p-GSK-3r1>, p-r1>-catenin, IGF-lR and GSK-3~ were significantly reduced ( P

Chuanshen 1 well, which is an ultra-deep prospecting well, has five-spud section drilled with the depth of 8 120 m. The fifth spud liner cementing confronted hash conditions such as ultrahigh temperature, ultra-high pressure, small annular space, great risk of gas channeling etc. Besides, the request of long term integrity and pack-off capability is higher for the cement sheath during production stage. A system of elastic anti-gas migration latex slurry is established in accordance,with the research of relationship between compressive strength of cement stone and dosage, particle size of silica sand, and with the research of influence on mechanic property by function material. The cement slurry system has stable performance, API Water loss is 18 mL, anti-channel coefficient SPN is 0. 89 elasticity, modulus is ·1. 8 GPa, permeability is 0.10 X 10³ μm², high-temperature settling stability is 0.01 g/cm³, and the compressive strength of 2,1 h is 16. 3 MPa. The cement system is successfully applied in super-deep small annular space liner cementing of Chuanshen 1 well combined with other technological measures, the cementing quality is excellent. [ABSTRACT FROM AUTHOR]

The aluminum alloy surfaces with circle-shaped micro cavities are fabricated by pulsed Nd : YAG laser. Under the conditions of sliding speed of 0. 15 m/s and 10 N load, a series of frictional experiments with uball- surface" friction pairs are conducted on a HSR-2M high-speed reciprocating friction and wear tester under the starved-oil lubrication condition. The influences of geometrical parameters of micro cavities on the surface of the circular cavity tribological properties are investigated. The influence of laser processing on the micro-hardness of aluminum alloy surface was analyzed and the wear characteristics o£ the test piece and the dual friction pair surface were studied by using the ultra-deep microscopy system. The results show that the micro-hardness of aluminum alloy substrates after laser processing is significantly improved. Under the starved-oil lubrication condition，the average friction coefficient of the micro-textured aluminum alloy surface is significantly reduced compared with the non-textured surface, and the fluctuation is more stable. According to the analysis of wear scar morphology, the surface wear of micro-textured aluminum alloy is reduced. When the diameter of the micro cavity is 60 pm and the areal density is 4. 91%，the surface o£ the micro-textured aluminum alloy has the best anti-friction and anti-wear properties. [ABSTRACT FROM AUTHOR]

Osteoporotic fracture is one of the most common skeletal system diseases in the elderly, which has the characteristics of high incidence, high disability rate, high mortality, and high medical cost. Because of the poor bone quality, comminuted fractures occur frequently, the stability of internal fixation is poor, bed stay time is long, and incidence of refracture is high. Therefore, the surgical treatment of osteoporotic fractures continues to challenge orthopedic surgeon. This paper reviews and summarizes the principles and techniques of surgical treatment for osteoporotic fractures in recent years. [ABSTRACT FROM AUTHOR]

[Objectives] To discuss the possibility of the non-destructive prediction of the total viable count of bacteria in the polluted eggs, this paper explored a non-destructive method that using near infrared hyperspectral imaging to predict and visualize the total viable count of bacteria in eggs. [Methods] Egg samples were inoculated with the mixture solution of Escherichia coli and Pseudomonas aeruginosa, then the original hyperspectral information and the total viable count of bacteria of eggs in different pollution levels during storage were collected. After selecting the best pretreatment method in this experiment, the predicting model by partial least squares (PLS) and support vector machine (SVM) model were developed based on full wavelengths and the feature wavelengths via the successive projections algorithm (SPA). Finally, the relative best prediction model of the viable count of bacteria was selected and the visualization study of the bacteria was realized in eggs. [Results] The second derivative method was the relative best pretreatment method, the correlation index of cross validation (RCV) was 0.88, and the root mean square error of cross validation was 0.82 lg (CFU·g-1). The relative best prediction model was the SVM model which was based on the feature wavelengths, the correlation index of calibration (RC) and prediction (RP) was 0.88 and 0.84, and the root mean square error of calibration and prediction was 0.86 and 0.97 lg (CFU·g-1), respectively. The visualized images of the polluted levels in eggs were built based on the difference of the pollution levels and spectral characteristics. [Conclusions] The near infrared hyperspectral imaging technology, and multivariate statistical and image processing methods can realize the prediction and visualization of the total viable count of bacteria in eggs, and this technology can also provide the foundation for on-line detection of the egg security. [ABSTRACT FROM AUTHOR]

There are about 1%-2% genomic DNAs encoding proteins in the human genome, and the coding products of the remaining 98% genomic DNAs are called noncoding RNAs (ncRNAs). NcRNAs play roles in many life activities including spermatogenesis. NcRNAs can regulate the gene expression at the transcriptional and post-transcriptional levels, in which ncRNAs participate in physiological and pathological processes such as gene imprinting, apoptosis, tumorigenesis and epigenetic modification. Spermatogenesis undergoes a long and complex change, from spermatogonial stem cells(SSC) to spermatozoa, which is regulated by multiple hormones and transcription factors. It has been found that ncRNAs also play some roles in spermatogenesis. NcRNAs can regulate the self-renewal and differentiation of SSC and participate in meiosis and other processes. NcRNAs are also expressed in spermatozoa. In this review, three kinds of ncRNAs and their effects in different stages of spermatogenesis were summarized. [ABSTRACT FROM AUTHOR]

The risk of kidney damage doubles during pregnancy. Acute kidney injury in pregnancy (P-AKI) is a high risk obstetrical disease that threatens the lives of mothers and their children. Despite the decline in the incidence of P-AKI in the past three decades, China still has a high incidence and mortality rate. P-AKI is a serious complication of pregnancy. It has clinical features of rapid onset, rapid progress and serious illness, often accompanied by respiratory, cardiovascular and digestive symptoms. P-AKI related diseases such as pre -eclampsia, acute fatty liver in pregnancy, HELLP syndrome and thrombotic microangiopathy have similar clinical manifestations and are prone to diagnosis difficulties. This article analyzes the pathophysiological changes, the incidence and causes of different countries, the diagnostic criteria, the related diseases of PAKI and the preventive and therapeutic measures, further analyzes the dialysis for P-AKI. It aims to improve the prevention and treatment of P-AKI, reduce the incidence, mortality and disability, and improve maternal and child outcomes. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to investigate the characteristics and effective factors of phytoplankton’s community structure in Dongji sea area of Zhoushan. [Method] Based on the continuous survey in four seasons on phytoplankton which were carried out from February, 2017 to October, 2017. The species composition, dominant species, abundance of phytoplankton, die diversity index and their relationships with environmental factors were analyzed. [Result]A total of 142 phytoplankton species were identified, which belong to 8 phyla, 70Taxa, including Bacillariophyta (47 genera and 104 species) , Pvrrophyta (12 genera and 27 species) , Gryptophyta (4 genera and 4 species) , Chiysophyta and Cyanophyta (2 genera and 2 species) , Chlorophyta, Xanthophyta and Euglenophyta (1 genera and 1 species) were found. Bacillariophyta and Pvrrophyta were the main phytoplankton, and there were totally 20 kinds of dominant species. The mean abun-dance of phytoplankton was 0.46 x 103 cells,abundance in summer and winter was obviously higher than in spring and autumn. The mean of phytoplankton’s Shannon-wiener diversity index was 3.07, the mean of Margalef richness index was 4.59, and the mean of Pielou evenness index was 0. 65. Three indexes show that the changing tendency was higher in autumn and winter and lower in spring and summer. [Conclusion] RDA analysis analysis indicated that the distributions of phytoplankton were related to water temperate, salinity , dissolved oxygen and nutrient. In addition, it may be affected by geographical location, climate change and anthropological activity. [ABSTRACT FROM AUTHOR]

As increasing reporting and disclosure of vulnerability code samples and extensive applications of machine learning methods, software vulnerability analysis methods based on machine learning have become a hot research direction in information security. After analysis of existing research work, we propose a software vulnerability analysis framework based on machine learning. We then review and classify existing machine learning based vulnerability methods, and conduct comparative analysis. We briefly analyze the challenges for machine learning based software vulnerability analysis methods, and discuss future research trends. [ABSTRACT FROM AUTHOR]

A reinforced concrete subsoil granary has been the promising type in agricultural production in recent years. Among them, a steel plate is used as the waterproof layer, which is connected to the reinforced concrete silo wall by welded bolts on the contact side of the steel plate and the reinforced concrete silo wall. The moisture-proof layer is taken as the aerated concrete blocks using epoxy structural adhesive. Specially, the aerated concrete block is bonded to the steel plate, thus forming a structural layer of epoxy structural adhesive bonding the steel plate and aerated concrete block. This study aims to evaluate the safety and reliability of this structural layer under the horizontal lateral pressure and friction of grain. The results are as follows: 1) Once the adhesive area was the same, there was the increase in the deformation, ultimate bearing capacity and bond strength at the interface of steel plate aerated concrete block under horizontal-vertical bidirectional loading, compared with the vertical unidirectional loading. The better bonding performance was then achieved under the horizontal lateral pressure that generated by the grain storage in the silo; 2) The damage pattern of vertical unidirectional loading was obtained, where the aerated concrete blocks were peeled off from the epoxy structural adhesive; In horizontal-vertical bidirectional loading, a penetrating crack was formed in the aerated concrete blocks, and then the whole block moved downward under the horizontal load, but without peeling off. A comparison was made on the damage patterns of vertical unidirectional loading and horizontal-vertical bidirectional loading. Therefore, it was beneficial to the stabilization of the interface, in the case of aerated concrete blocks under vertical loading while applied horizontal lateral pressure. 3) The bond strength was closely related to the friction force on the silo wall, when the adhesive area was A, 0.8A, 0.5A (A was the contact area between a single aerated concrete block and a steel plate). Once the adhesive area was 0.5A, the smallest height of grain storage was achieved under the vertical unidirectional loading, indicating the larger than the actual height. Therefore, the structural layer with the gluing area of 0.5A was fully met the requirements of the bearing capacity and bonding strength under grain storage loads in the design of underground granaries. 4) It was feasible to adopt the construction practice of epoxy structural adhesive bonding steel plates and aerated concrete blocks for underground granaries under the horizontal lateral pressure and vertical friction of grain. 5) The bearing capacity and bond strength were fully met the requirements, when the bonding area of epoxy structural adhesive exceeded 50% of the total area of aerated concrete block. Further consideration should be given to the influence of the epoxy structural adhesive thickness and application mode on the displacement, bearing capacity, bond strength and damage pattern of the structural layer. The findings can provide a strong reference for the safety and reliability of the waterproof and moisture-proof structural layer in the new underground granary. [ABSTRACT FROM AUTHOR]

目的观察不同介入时机行持续性肾脏替代治疗(CRRT)对剖宫产术后急性肾损伤(AKI)患者肾功能的影响。方法选择剖宫产术后发生AKI的产妇87例,随机分为早期组30例、中期组30例、晚期组27例。早期组确诊12 h内行CRRT,中期组确诊12～24 h内行CRRT,晚期组确诊24 h后行CRRT。比较各组治疗前及治疗第3、6天血清尿素氮(BUN)、肌酐(Cr)、β2微球蛋白(β2-MG)及胱抑素C(Cys C),尿量恢复时间(尿量恢复至60 mL/h)及ICU住院时间,气管插管率及随访半年内慢性肾炎发生率、生存情况。结果与治疗前比较,三组治疗后第3、6天BUN、Cr、β2-MG、Cys C均降低(P均<0. 05)。早期组、中期组治疗前及治疗后第3、6天BUN、Cr、β2-MG均低于晚期组,中期组治疗前及治疗后第3天Cys C均低于早期组和晚期组,早期组、中期组治疗后第6天Cys C均低于晚期组(P均<0. 05)。早、中、晚期组尿量恢复时间及ICU住院时间均依次升高(P均<0. 05)。晚期组气管插管率高于早期组、中期组(P均<0. 05),三组慢性肾炎发生率比较差异无统计学意义(P均>0. 05)。三组均无死亡病例。结论剖宫产术后AKI患者早期进行CRRT有助于肾功能的恢复,并可缩短病程、降低气管插管率。 [ABSTRACT FROM AUTHOR]

Objective To observe the clinical efficacy of arthroscopic minimally invasive surgery combined with sodium silicate and warm acupuncture in the treatment of knee osteoarthritis (KOA). Methods A total of 73 KOA patients who met the inclusion criteria from June 2015 to June 2017 were enrolled in the study. They were randomly divided into the treatment group (n = 39) and the control group (n = 34 ) . The control group was treated with intra articular injection of sodium hyaluronate and warm acupuncture. In addition to the above treatment, the treatment group assisted arthroscopy. The visual analogue scale ( VAS score) and the Lysholm knee function score were compared between the two groups. By comparing the levels of serum transforming growth factor β (TGF β) and insulin like growth factor 1 (IGF 1) before and after treatment, the clinical effects of the two groups before and after treatment were studied. Results Compared with before treatment, the VAS scores of the two groups were followed up at 1, 3, 6 and 12 months after treatment, and the differences were statistically significant ( P < 0.05 ) . The Lysholm knee fuction scores in both groups showed an upward trend, and the scores of the treatment group were better than that in the control group, and the differences were statistically significant ( P < 0 05). The serum levels of TGF β and IGF 1 were higher in the two groups than in the control group, and the differences were statistically significant ( P < 0.05). Conclusion Arthroscopic debridement combined with sodium hyaluronate and warm acupuncture for the treatment of knee osteoarthritis can significantly relieve symptoms, improve joint function, and is associated with elevated serum levels of TGF β and IGF 1. [ABSTRACT FROM AUTHOR]

Objective To systematically evaluate the clinical efficacy of ABO-incompatible living donor liver transplantation (ABO-I LDLT) and compare with ABO-compatible LDLT (ABO-C LDLT). Methods A systematic search of multiple databases at home and abroad was conducted to retrieve the literatures related to the statistical comparison of clinical efficacy between ABO-I LDLT and ABO-C LDLT. The literature screening was conducted, the quality of literatures was evaluated and data extraction was performed. Using Rev Man 5.3 software, a Meta-analysis was performed by random effect model or fixed effect model. Results A total of 432 articles were searched, and 6 articles published in English were eventually included according to the inclusion criteria. The Meta-analysis demonstrated that there was no significant difference in the postoperative 1-, 3- and 5-year survival rate of the recipients and grafts and the incidence of rejection responses between the ABO-I LDLT and ABO-C LDLT groups (all P≥0.05). The incidence of postoperative biliary complications and hepatic artery embolization in the ABO-I LDLT group was significantly higher than that in the ABO-C LDLT group [odds ratio (OR) =2.08, 95% confidence interval (CI) 1.25-3.45, P=0.005; OR=2.24, 95%CI 1.03-4.89, P=0.04]. Conclusions Compared with the ABO-C LDLT, ABO-I LDLT yields lower clinical efficacy, whereas it is still an effective method for the treatment of end-stage liver disease. [ABSTRACT FROM AUTHOR]

[Objectives] This study was conducted to investigate the effect of different levels of dietary palygorskite supplementation on pellet quality, growth performance, slaughter performance, meat quality and antioxidant ability in Cherry Valley ducks. [Methods] According to the randomized block design, a total of 720 one-day-old Cherry Valley ducks were randomly divided into 4 dietary treatment groups with 6 replicates of 30 ducks per replicate, and fed a basal diet supplemented with 0(control group), 5, 10 and 20 g·kg-1 palygorskite. The experiment period was 42 days, which consisted of the starter period(1 to 14 days)and grower period(15 to 42 days). At the end of the experiment, 1 male duck per replicate was randomly selected for measuring the relevant indexes. [Results] Compared with the control group, three levels palygorskite supplementation increased the hardness and decreased the fines percentage of pellet during starter period(P < 0.05). The inclusion of 20 g·kg-1 palygorskite increased the pellet hardness of pellet during grower period(P < 0.05), and other levels palygorskite supplementation did not affect the pellet hardness and the fines percentage of pellet during grower period(P>0.05). Palygorskite supplementation, regardless of levels, did not affect the final body weight, average daily feed intake, average daily weight gain and the ratio of feed intake to gain(F/G)of ducks. However, the percentage of breast muscle, leg muscle and lean muscle increased when supplementing 10 g·kg-1 palygorskite(P < 0.05), and the inclusion of 20 g·kg-1 palygorskite increased the percentage of leg muscle and lean muscle(P < 0.05), but had no effect on the percentage of breast muscle(P>0.05). 10 g·kg-1 palygorskite supplementation showed higher leg muscle percentage than 20 g·kg-1 palygorskite supplementation(P < 0.05). In addition, three levels palygorskite supplementation all significantly decreased the drip loss of breast muscle(P < 0.05). The inclusion of 20 g·kg-1 palygorskite decreased the cooking loss of breast muscle(P < 0.05). Similarly, the redness(a*)of breast muscle and the drip loss of leg muscle decreased significantly(P < 0.05)when the palygorskite inclusion dosage was 10 g·kg-1. 10 and 20 g·kg-1 palygorskite supplementation all significantly increased the superoxide dismutase(SOD)activity of breast muscle(P < 0.05), and 5 g·kg-1 palygorskite supplementation increased the glutathione peroxidase(GSH-Px)activity of breast muscle(P < 0.05). The inclusion of 10 g·kg-1 palygorskite supplementation increased the superoxide dismutase activity of leg muscle. Whereas the malonaldehyde(MDA)contents of leg muscle decreased significantly(P < 0.05)by three levels palygorskite supplementation. [Conclusions] The basic dietary palygorskite supplementation could improve the feed pellet quality, enhance slaughter performance and meat quality, and increase antioxidant ability, and had no disadvantageous effect on the growth performance of Cherry Valley ducks. In addition, the optimal level of supplemented palygorsskite in the pellet diets of ducks was 10 g·kg-1. [ABSTRACT FROM AUTHOR]

Network and information systems are developed as the core of key infrastructures, economy and society. Once such systems are attacked by adversaries or severe security events, the security of national economy and the common value of the society can suffer from adverse impacts. Hence, how to detect network threats and how to ensure the security of network infrastructure are vital for protecting key technologies and constructing national cybersecurity assurance systems. We systematically review several studies on intrusion detection framework, automatic signature generation, security detection theories and methods, network topology monitoring and routing control. Furthermore, we summarize several scientific findings, such as cybersecurity detection algorithm and framework, wireless network security detection, network monitoring and security enhancement. Finally, we discuss several interesting future directions on cybersecurity detection and control. [ABSTRACT FROM AUTHOR]

Objective To explore the influence of Wuhuang decoction lavage on the expression of IT-8 and intercellular cell adhesion molecule-1 (ICAM-1) in sinus secretions of fungal rhinosinusitis (FRS) after operation. Methods A randomized controlled trial was used ,60 FRS patients after nasal endoscopic surgery,who were received in the hospital from January 9th 2013 to September 30th 2016,were divided into the treatment group and the control group according to the random number generated by computer,30 cases in each group. The patients in the treatment group were lavaged with 100 mL Wuhuang decoction through sinuses,once a day for 4 weeks as a period; the patients in the control group were lavaged with fluconazole injection,the frequency and the dosage were the same as the treatment group. The expression of IT-8 and ICAM-1 in sinuses secretion before and 1 month after treatment were determined, moreover the efficacy was evaluated by endoscopic examination 6 months after operation. Simultaneously,5 healthy participants from the school were enrolled as the healthy control group,the expression of IT-8 and ICAM-1 were detected in 1 mC recycling residual in nasal cavity after lavaging with 100 mh normal saline. Results The difference had statistical significance (p

To remedy the limitation of the existing outer rotor scheme which increases the unbalance of rotor system due to screw connection, a vernier gimballing magnetically suspended spherical flywheel with inner rotor based on reluctance force and Lorentz force hybrid configuration is presented. The structure and working principle of the flywheel are introduced, and the control model of rotor system is analyzed. It is concluded that the disturbance of translation suspension on radial tilting control can be eliminated and the controller can be simplified as well when the centroid of rotor coincides with the geometrical symmetrical center of rotor and testing center. In consideration of the maximum equivalent stress, the first-order resonance frequency, the ratio of polar moment of inertia to equinoctial inertial moment, the polar moment of inertia, the maximum deformation and the deviation of the centroid of rotor and the sphere center of rotor are increased as constraint condition. The multidisciplinary optimization design of rotor system is performed by taking the high sensitivity parameters as optimal design variables and the minimum rotor mass as the optimization objective. The results indicate that the mass of rotor is decreased from 5.600 kg to 5.389 kg, which is reduced by 3.8%, under the condition satisfying the design requirements. The optimization design method can improve the design efficiency and control torque precision of flywheel system. [ABSTRACT FROM AUTHOR]

目的 探讨过氧化物酶体增殖物激活受体家族(PPARs)激动剂非诺贝特对动脉粥样硬化形成的影响及作用机制.方法 将25只雄性新西兰白兔随机分为五组,每组5只,A组予标准饲料喂养,其余四组予高脂饲料喂养,均喂养8周.C、D、E组喂养4周结束时均行经股动脉球囊损伤术,并分别于手术当天给予生理盐水、非诺贝特、WY14643(非诺贝特酸的人工合成物)连续4周.喂养8周结束时,五组均进行股动脉内膜剥脱术,取股动脉,并留取血清.取各组股动脉标本,HE染色后显微镜下观察血管形态,计算血管狭窄率;采用免疫组化SP法及Western blotting法检测股动脉PPARα蛋白表达;采用RT-PCR法检测股动脉PPARα mRNA表达;采用ELISA法检测血清TNF-α、IL-10和P-选择素水平.结果 C、D、E组股动脉均有动脉硬化表现,D、E组表现均较C组轻,且E组较D组轻.与A组比较,其余四组管腔狭窄率均升高(P<0.05或<0.01);与C组比较,D、E组管腔狭窄率降低(P均<0.05).D、E组股动脉PPARα蛋白和mRNA表达均高于C组,血清IL-10水平高于C组,血清TNF-α和P-选择素水平均低于C组;组间比较P<0.05或<0.01.结论 非诺贝特可减轻由高脂饲料和球囊损伤引起的兔动脉粥样硬化病变程度,增加IL-10表达、减少TNF-α和P-选择素表达,抑制炎症反应可能是其作用机制. [ABSTRACT FROM AUTHOR]

Nowadays, wearable devices are widespread in our daily life. The rapid growth of users' number results in data re-dissemination by data holders. Nevertheless, improper re-dissemination methods can lead to unacceptable information loss or privacy leakage. In addition to privacy preserving concern, the data republishing methods for wearable devices should also be efficient enough due to the rapid increase of the number of wearable devices. In order to enhance the efficiency under the premise of information security of users and acceptable information loss, we propose a wearable data re-dissemination method based on clustering K-anonymity. The proposed method jointly considers data privacy, information loss and the overheads. Specifically, the proposed method processes the incremental data directly to enhance the efficiency and the clustering K-anonymity can limit information loss. The proposed method can reduce information loss when the amount of the data is huge. Experimental results demonstrate its effectiveness. [ABSTRACT FROM AUTHOR]

Aquaculture park is an important form to promote scale breeding, which has an important significance for the transformation of aquaculture production style and the structural reform of supply side. In this paper, we use the stochastic frontier approach to analyze the production efficiency of aquaculture park with large scale in Zhejiang Province and its influencing factors. The stochastic frontier analysis method is widely used in the studies on agricultural production, yet the research using this method to calculate the technology efficiency of aquaculture park and analyze its influencing factors is relatively few. The technical efficiency of tilapia culture in Guangxi and the technical efficiency of freshwater aquaculture in China have been studied by some scholars. However, most of these studies are limited to a single aquatic species or based on macro data, lacking the analysis of the operating efficiency of the micro main varieties. This paper chooses Zhejiang Province as the research area because of its developed aquaculture industry, typical park construction, standard breeding and industrial organization system. Based on previous studies, we investigate the technical efficiency of aquatic breeding park and its influencing factors, which aimed to provide reference for the healthy development of the aquaculture industry in China. The data of empirical analysis come from the Agriculture Department and the Financial Department of Zhejiang Province, which cover 116 modern fishery parks. Cross-sectional data of both before and after the construction of modern fishery park are collected. Statistical indicators mainly include the scale and efficiency (breeding area, total production, total output value, labor productivity per capita), capital investments (provincial capital investment, capital investment of owners), facilities and equipment investments (reservoir, cold storage preservation, supporting house for production, oxygen increasing machine, feeding machine), technology application and brand building (standardized production area, sewage purification treatment, disease prevention and treatment, aquatic products brand), and business entities (main operating company, cooperatives and farmers). Case data are discarded if key information is missing. At last, a total of 108 valid cases are obtained. In order to eliminate the impact of price changes, the output value of fishery park, the capital investments and other variables are divided by production price index. The results show that: (1) Scale production exerts a positive effect on the aquaculture production efficiency which has increased from 0.673 to 0.712 since the completion of the park; (2) The standardization level effectively improves the breeding technology efficiency of the park, with a regression coefficient of -0.0003; (3) Disease control equipment improves the technical inefficiency (the regression coefficient is 0.0033), which means that excessive investment in infrastructure will lead to idle equipment and hinder the improvement of technical efficiency; (4) Brand building positively influences the breeding technology efficiency of the park, with a regression coefficient of -0.0033; (5) Leading enterprises have the greatest positive effect on the level of technical efficiency, followed by the organization of fishery cooperation and the large professional farmers, with the regression coefficients of technical inefficiency being -0.0017, 0.0015 and -0.0008 respectively. But the regression coefficient of ordinary farmers is 0.0012, which implies no significant effect on the efficiency of aquaculture technology. Some policy implications can be drawn from the results of the empirical study. Increasing production of aquaculture park inputs, investing infrastructure following principle of moderate scale, paying attention to brand building and innovating the mode of fishery organization and so on may enhance the technical efficiency of aquaculture park. [ABSTRACT FROM AUTHOR]

Objective To explore the optimal scheme of Chinese medicine Wuhuang decoction for nasal lavage after operation of chronic rhino-sinusitis. Methods 45 patients with chronic rhino-sinusitis diagnosed by otorhinolaryngology Department of the Affiliated Hospital of Traditional Chinese University from April to December 2017 were selected and randomly divided into treatment group 1, treatment group 2 and treatment group 3, each group of 15 patients, who were treated with Wuhuang decoction for nasal lavage. The scores of conscious symptoms, tolerance to nasal lavage and endoscopic Lund-Kennedy were given at half month, one month and three months after operation respectively. Results At half a month of lavage, one month of lavage and three months after operation, the scores of conscious symptoms and Lund-Kennedy scores of nasal endoscopy in the treatment group 1 and the treatment group 2 were lower than those in the treatment group 3, and the differences were statistically significant (P<0.05). At 1 month of lavage and 3 months after operation, there was no significant difference in the scores of conscious symptoms between the treatment group 1 and the treatment group 2(P>0.05). At 1 month of lavage, the tolerance score of nasal lavage in the treatment group 2 was lower than that in the treatment group 1 and the treatment group 3, and the difference was statistically significant (P<0.05). Conclusion The best treatment for chronic rhino-sinusitis after operation is to lavage once a day in the first half month and once every other day. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of RNA interference CMTM7 on apoptosis of lung adenocarcinoma A549 cell by the. Methods: Selected lung adenocarcinoma A549 cell were divided into si RNA group, negative control group and blank control group,and the RNA interference CMTM7 vectors were transfected into the logarithmic growth of A549 cells, observed A549 cell growth, and the apoptosis and cell cycle status was detected. Results: MTT experiment showed that the inhibition rate in si RNA transfection group was significantly higher than that of the negative control group and blank control group(P>0.05), while the difference between the negative control group and the control group were not significant(P<0.05). Flow cytometry showed the apoptosis rate in the si RNA transfection group was significantly higher than that of the negative control group and blank control group(P<0.05), while the difference between the negative control group and the control group were not significant(P>0.05). Flow cytometry experiments showed that the number of G0 / G1 phase cell in the si RNA transfection group was increased with significantly difference compared with blank control group and negative control group(P<0.05), while the number of G2 / M phase cell in the si RNA transfection group was less than that of the control group and negative control group(P>0.05), but it had no significant difference between the blank control group and negative control group(P>0.05). Conclusions: RNA interference CMTM7 can promote lung adenocarcinoma A549 cell apoptosis, inhibit the growth of tumor cells, and its mechanism may be achieved by interfering with the cell cycle. [ABSTRACT FROM AUTHOR]

To solve the poor covert of arrive sonar detection system, a bionic active sonar covert detection technology is proposed on the basis of dolphin whistle fiignals. At the transmitting terminal, the LFM sig­nals arc hidden in dolphin whistles to design an covert active sonar hinnic detection signal. This signal is similar to dolphin whistles in the ocean, so we can use the biological environmental noise in (he ocean tu achieve active sonar covert detection. At the receiving terminal, the adaptive correlator is used as an ac­tive sonar processor. The udaplive correlator has a strong ability to match the marine environment-Tt can uulomutieully mulch the murine channels, and reduce the multipath spreading loss effectively, thus getting Liyjiri signal to noise ratio processing guru than the matching Glter, The adaptive correlator contrib­utes to long-range detection. The simulation and the sea experiment proof the eoverlness and effectiveness of The proposed bionic active sonar covert detection technology based on dolphin whistle signals. [ABSTRACT FROM AUTHOR]

[Objectives] The aim of the study was to investigate the effects of guanidinoacetic acid (GAA) on growth performance, slaughtering performance, and antioxidant capacity in Cherry Valley ducks. [Methods] A total of 720 healthy 10 day-old Cherry Valley ducks with roughly equal weight were randomly assigned to 4 groups with 6 replicates of 30 ducks in each replicate (pen). Ducks were fed a basal diet supplemented with 0% (control), 0.025% (experiment I), 0.05% (experiment II) and 0.10% (experiment III) GAA. At 42 day-old, 1 duck per pen was randomly selected for measuring the relative indexes. [Results] GAA supplementation tended to increase average daily gain (ADG) and decrease feed gain ratio (F/G) respectively, but it was not significant (P>0.05). The percentage of half eviscerated yield, eviscerated yield, and leg muscle also tended to be increased, especially in the experiment II (P<0.05), compared with the control. The creatine content of breast muscle in experiment II and the plasma creatine content in experiment III were higher (P<0.05) than those in control. In serum, total antioxidant capacity(T-AOC), catalase (CAT) and superoxide dismutase (SOD) activity in the three experiment groups were numerically increased(P>0.05)and malondioldehyde (MDA) content tended to decrease, especially in experiment II (P<0.05), compared with the control. Compared with the control, glutathione peroxidase (GSH-Px)activity in the three experiment groups were increased respectively by 9.96%, 32.46%, 12.34%, and the experiment II was significantly higher than other groups (P<0.05). Reduced glutathione tablets (GSH) content was markedly increased (P<0.05) in experiment Ⅱ compared with the control. In liver, the activity of T-AOC, CAT, SOD, GSH-Px and the content of GSH tended to be increased when GAA was supplemented, especially the SOD activity and GSH content in the experiment II, which were higher (P<0.05) than the other groups, and the GSH-Px activity was higher (P<0.05) than the control. [Conclusions] The results suggest that diets supplemented with GAA has no significant effect on growth performance of Cherry Valley ducks, but can improve slaughter performance and antioxidant capacity. Under this experiment conditions, the optimum addition content of GAA in diets was 0.05%. [ABSTRACT FROM AUTHOR]

目的探讨颈动脉粥样硬化斑块影像学特征及血清炎症因子与老年脑梗死发病的相关性。方法采用彩色多普勒超声检测的老年脑梗死患者100例,其中首发急性脑梗死患者50例(首发脑梗死组),继发性脑梗死患者50例(继发脑梗死组),分析患者颈动脉超声的特征及斑块性质。采用ELISA法检测脑梗死患者血清中脑钠肽(BNP)、脑钠肽前体(NT-pro BNP)、炎症小体3(NLPR3)、凝集素样氧化型低密度脂蛋白受体(LOX-1)、IL-1β水平。以本院同期健康体检者130例作为对照组。结果与对照组比较,老年脑梗死患者颈动脉内-中膜厚度、颈动脉内径、颈动脉管壁增厚比例和颈动脉内膜毛糙、回声增强比例高,颈动脉粥样斑块检出率高,血清中BNP、NT-pro BNP、NLPR3、LOX-1、IL-1β水平升高,动脉粥样硬化危险因素如高血压史、糖尿病史的发生率高,血清总胆固醇、甘油三酯、低密度脂蛋白水平高,高密度脂蛋白水平低(P均0.05)。首发与继发脑梗死组比较,P均0.05。结论颈动脉粥样硬化斑块影像学特征及血清炎症因子与老年脑梗死发病均有关。 [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of sevoflurane combined with remifentanil on neurologic function, cognitive function and inflammatory factors in patients with intracranial aneurysms undergoing interventional therapy. Methods: 80 patients with intracranial aneurysms who underwent interventional surgery in our hospital from January 2020 to June 2022 were selected and divided into group A(sevoflurane combined with remifentanil) and group B(propofol combined with remifentanil) according to different anesthesia methods, with 40 patients in each group. Neurological function, cognitive function and inflammatory factor level were compared between the two groups. Results: The heart rate(HR) and mean arterial pressure(MAP) of group A were better than those of group B from T1 to T3(P＜0.05). The national institutes of health stroke scale(NIHSS) score of group A was significantly lower than that of group B at 24 h and 7 d after operation(P＜0.05), and the mini-mental state examination(MMSE) score was higher than that of group B(P＜0.05).Serum tumor necrosis factor-α(TNF-α), C-reactive protein(CRP), and interleukin-6(IL-6) levels at 24 h and 3 d after operation in group A were lower than those in group B(P＜0.05). The recovery time of spontaneous respiration, recovery time, extubation time and the incidence of agitation after extubation in group A were lower than those in group B(P＜0.05). The adverse effects were not different(P＞0.05).Conclusion: The application of sevoflurane combined with remifentanil maintenance anesthesia in interventional IA patients can smooth hemodynamics, improve postoperative neurological function and cognitive function, reduce inflammatory response, and improve the quality of anesthesia awakening. [ABSTRACT FROM AUTHOR]

目的 探讨仰卧位后伸定点旋转扳法治疗颈型颈椎病的效果及作用机制.方法 选取颈型颈椎病患者80例,随机分为观察组和对照组各40例,观察组给予仰卧位后伸定点旋转扳法进行理疗康复,对照组给予传统推拿法进行康复.两组治疗前后均行颈椎X线检查,统计颈椎曲度改变、骨质增生、韧带钙化、椎体紊乱例数;采用皮尺测量颈椎活动度,包括前驱、后伸、左右侧屈、左右旋转体位下颈椎活动度;采用ELISA试剂盒检测血清IL-2、IL-6、TNF-α;评价治疗效果,统计两组治疗后优良率.结果 观察组治疗后颈椎曲度变直、椎体紊乱例数均少于对照组(P均<0.05),两组骨质增生、韧带钙化例数比较差异无统计学意义(P均>0.05).两组治疗后前屈、后伸、左旋、右旋、左侧屈、右侧屈颈椎活动度均较治疗前增加,观察组增加更显著(P均<0.05).两组治疗后血清IL-2、IL-6、TNF-α水平均下降,观察组下降更显著(P均<0.05).观察组治疗优良率为82.5%,对照组为67.5%,两组比较P<0.05.结论 仰卧位后伸定点旋转扳法可促进颈型颈椎病患者颈椎活动度恢复,减轻炎症反应可能是其作用机制. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of lycopene（Lyc）on the proliferation，migration and tube-forming capacity of human endothelial progenitor cells（EPCs）and to explore their mechanisms. Methods EPCs were cultured for 24 h and then were divided into 4 groups. In the group A，the medium containing 10% fetal bovine serum and ox-LDL （10 mg/L）were added；after 24 h of culture，the supernatant was discarded，and the same serum and Lyc（10 mg/L） were added for 24 h of culture. In the group B，EPCs were cultured in the medium containing the same fetal bovine serum and ox-LDL（100 mg/L）for 48 h. EPCs in the group C were cultured with the same serum and Lyc（10 mg/L）for 48 h. scratch healing rate，tubule diameter and relative protein expression levels of p62，LC3-Ⅱ and Beclin-1 were compared among groups. Results Compared with the group B，cell proliferation activity， cell scratch healing rate，tubular diameter and relative expression levels of LC3-Ⅱ，Beclin-1 and p-AMPK/AMPK increased in the group A，while the relative expression levels of p62 and p-mTOR/mTOR decreased（all P<0. 05）. Compared with the group B，the cell proliferation activity，cell scratch healing rate，tubular diameter and p-AMPK/AMPK protein relative expression levels increased in the group D，while the protein relative expression levels of p62，LC3-Ⅱ，Beclin- 1 and p-mTOR/mTOR decreased（all P<0. 05）. Compared with the group F，the cell proliferation activity，cell scratch healing rate and tubular diameter decreased in the groups E and G（all P<0. 05）. Compared with the group G，the relative expression level of p62 protein increased in the group E，but the relative expression levels of LC3-Ⅱ and Beclin-1 protein and the relative expression levels of p62，LC3-Ⅱ and Beclin-1 protein in the group H decreased（all P<0. 05）. Conclusions Lyc can promote the proliferation，migration and tube formation of EPCs，and enhance autophagy. The mechanism may be that it up-regulates AMPK phosphorylation and down-regulates mTOR phosphorylation，thereby inhibiting mTOR signaling pathway. [ABSTRACT FROM AUTHOR]