147 results on '"Guangdong Yang"'
Search Results
2. Secure computing: logistic regression analysis with differential privacy
- Author
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Yao Qiu, Shulan Wang, Caiguo Li, Guangdong Yang, Haiyan Wang, Fucai Luo, and Yong Ding
- Published
- 2023
3. Genome-wide identification and expression analysis of the CHYR gene family in Phaseolus vulgaris under abiotic stress at the seeding stage
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Xianxin Meng, Zhengong Yin, Guangdong Yang, Shuhong Wei, Yifan Guo, Wei Zhang, and Qiang Wang
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Plant Science ,Ecology, Evolution, Behavior and Systematics - Published
- 2023
4. Spatial migration and distribution of suspended particles in saturated sand layer under the impact of temperature
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Xianze Cui, Jin Li, Guangdong Yang, Wenbin Fei, Shengyong Ding, and Yong Fan
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Water Science and Technology - Published
- 2023
5. Integrated Utilization of Vanadium-Titanium Magnetite Tailings for Synthesis of Lightweight Foamed Ceramics: Effect of Chemical Composition on the Properties and Phase Evolution
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Lin Li, Tao Jiang, Bojian Chen, Jing Wen, and Guangdong Yang
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Mechanics of Materials ,Metals and Alloys ,Environmental Science (miscellaneous) - Published
- 2022
6. H2S protects from oxidative stress-driven ACE2 expression and cardiac aging
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Kalem Barrow, Yuehong Wang, Ruihuan Yu, Jiechun Zhu, and Guangdong Yang
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Clinical Biochemistry ,Cell Biology ,General Medicine ,Molecular Biology - Published
- 2022
7. Phase Evolution Behavior and Oxidation Induration Mechanism of High-Chromium Vanadium–Titanium Magnetite Flux Pellets
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Bojian Chen, Tao Zhao, Guangdong Yang, Jing Wen, Li Lin, and Tao Jiang
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Materials science ,digestive, oral, and skin physiology ,Metals and Alloys ,Pellets ,Vanadium ,chemistry.chemical_element ,Slag ,Condensed Matter Physics ,chemistry.chemical_compound ,Chromium ,Compressive strength ,chemistry ,Chemical engineering ,Mechanics of Materials ,visual_art ,Materials Chemistry ,visual_art.visual_art_medium ,Porosity ,Magnetite ,Titanium - Abstract
The quality of vanadium–titanium magnetite sinters is poor. The high-pellet operation of the blast furnace ironmaking process is a feasible method for utilization of the High-chromium Vanadium–titanium magnetite (HVTM). To ensure the basicity of the burden, flux pellets need to be investigated. In this study, HVTM concentrate and CaCO3 were used to prepare flux pellets. The phase transition and oxidation induration mechanism of flux pellets were studied separately and analyzed by scanning electron microscopy and energy dispersive spectroscopy. Solid-phase and solid–liquid-phase experiments were used to verify the phase transition behavior. The results indicated that the basicity (CaO/SiO2) increased from 0.4 to 1.4, and the compressive strength of HVTM concentrate flux pellets first increased to 4916 N and then decreased to 2399 N at 1523 K for 20 minute. The porosity of the pellets increased from 8.236 to 20.734 pct. Perovskite produced due to increasing basicity reduced the slag content and had a low bonding strength, which reduced the overall compressive strength of the pellets. The increasing basicity was conducive to the solid solution of Ti forming an iron-titanium solid solution. The addition of CaCO3 increased the porosity of the pellets. The oxidation induration of the pellets changed from oxide-bonded to slag-bonded.
- Published
- 2021
8. Analysis of Toe Formation in Stress-Concentration Zones on High Rock Slopes
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Peng Yan, Zhendong Leng, Guangdong Yang, Ming Chen, and Ke Deng
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Stress (mechanics) ,Linear density ,Computer simulation ,Crack initiation ,Abutment ,Geotechnical engineering ,Fracture mechanics ,Lateral side ,Geology ,Civil and Structural Engineering ,Stress concentration - Abstract
Toe formation is a phenomenon resulting from unconnected cracks between blastholes, which occurred during blasting excavation of the abutment slot at Baihetan hydropower station. Analysis of crack development has tended not to focus on the high in-situ stress environment at the slope corner. Therefore, this study aimed to explore the toe formation process through theoretical analysis and numerical simulation. Firstly, a three-blasthole model was established to analyze the effects of blasting load and in-situ stress on crack extension. Then, a finite-element model of the abutment slot was built to simulate crack propagation under different in-situ stress environment. Finally, optimization measures for avoiding toe were proposed. The results demonstrate that cracks grow preferentially in the direction of the maximum tangential tensile stress and are influenced by the blasting load and in-situ stress. On the lateral side of the abutment slot, the crack initiation and extension occur far from the centerline due to the deviation of the maximum tangential tensile stress. The deviation increased as the in-situ stress. Consequently, cracks between blastholes not being able to connect with each other results in toe formation. Increment of the linear density of the charge and optimization of the excavated profile are both effective in reducing the impact of the stress concentration caused by in-situ stress and preventing toe formation.
- Published
- 2021
9. H2S signaling and extracellular matrix remodeling in cardiovascular diseases: A tale of tense relationship
- Author
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Jiechun Zhu and Guangdong Yang
- Subjects
0303 health sciences ,Cancer Research ,Physiology ,Cellular differentiation ,Clinical Biochemistry ,Cell migration ,Inflammation ,030204 cardiovascular system & hematology ,Biology ,medicine.disease_cause ,medicine.disease ,Biochemistry ,3. Good health ,Cell biology ,Extracellular matrix ,03 medical and health sciences ,0302 clinical medicine ,Fibrosis ,medicine ,medicine.symptom ,Cell adhesion ,Oxidative stress ,Tissue homeostasis ,030304 developmental biology - Abstract
Extracellular matrix (ECM) is a non-cellular three-dimensional macromolecular network that not only provides mechanical support but also transduces essential molecular signals in organ functions. ECM is constantly remodeled to control tissue homeostasis, responsible for cell adhesion, cell migration, cell-to-cell communication, and cell differentiation, etc. The dysregulation of ECM components contributes to various diseases, including cardiovascular diseases, fibrosis, cancer, and neurodegenerative diseases, etc. Aberrant ECM remodeling is initiated by various stress, such as oxidative stress, inflammation, ischemia, and mechanical stress, etc. Hydrogen sulfide (H2S) is a gasotransmitter that exhibits a wide variety of cytoprotective and physiological functions through its anti-oxidative and anti-inflammatory actions. Amounting research shows that H2S can attenuate aberrant ECM remodeling. In this review, we discussed the implications and mechanisms of H2S in the regulation of ECM remodeling in cardiovascular diseases, and highlighted the potential of H2S in the prevention and treatment of cardiovascular diseases through attenuating adverse ECM remodeling.
- Published
- 2021
10. H2S-mediated blockage of protein acetylation and oxidative stress attenuates lipid overload-induced cardiac senescence
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Guangdong Yang, Ruihuan Yu, Yuehong Wang, and Jiechun Zhu
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Senescence ,Physiology ,medicine.disease_cause ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Physiology (medical) ,medicine ,Viability assay ,030304 developmental biology ,0303 health sciences ,biology ,Chemistry ,Cystathionine gamma-lyase ,General Medicine ,equipment and supplies ,Cystathionine beta synthase ,3. Good health ,Cell biology ,Lipotoxicity ,biology.protein ,lipids (amino acids, peptides, and proteins) ,030217 neurology & neurosurgery ,Intracellular ,Oxidative stress - Abstract
Hydrogen sulphide (H2S), a newly identified gasotransmitter, can be endogenously produced by cystathionine gamma-lyase (CSE) in the cardiovascular system. This study investigated the role of the CSE/H2S system on lipid overload-induced lipotoxicity and cardiac senescence. Lipid overload in rat cardiomyocyte cells (H9C2) promoted intracellular accumulation of lipid, oxidative stress, mitochondrial dysfunctions, lipid peroxidation and inhibited cell viability, all of which could be reversed by exogenously applied H2S. Further data revealed that H2S protected H9C2 cells from lipid overload-induced senescence by altering the expressions of lipid metabolism-related genes and inhibiting cellular acetyl-CoA and global protein acetylation. Enhancement of protein acetylation abolished the protective role of H2S on cardiac senescence. In vivo, knockout of the CSE gene strengthened cardiac lipid accumulation, protein acetylation, and cellular ageing in high fat diet-fed mice. Taken together, the CSE/H2S system is capable of maintaining lipid homeostasis and cellular senescence in heart cells under lipid overload.
- Published
- 2021
11. Effect of hydrogen sulfide on glycolysis‐based energy production in mouse erythrocytes
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Guangdong Yang, Ming Fu, Lingyun Wu, Rui Wang, Eden T Wondimu, Roberta Torregrossa, Zhuping Jin, Quanxi Zhang, and Matthew Whiteman
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Erythrocytes ,Physiology ,Clinical Biochemistry ,Endogeny ,Mitochondrion ,7. Clean energy ,Mice ,03 medical and health sciences ,Adenosine Triphosphate ,0302 clinical medicine ,medicine ,Animals ,Glycolysis ,Hydrogen Sulfide ,Viability assay ,Hypoxia ,030304 developmental biology ,Mammals ,0303 health sciences ,Chemistry ,Cell Biology ,Metabolism ,Cell biology ,Red blood cell ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Erythropoiesis ,Intracellular - Abstract
Hydrogen sulfide (H2 S) is a gasotransmitter that regulates both physiological and pathophysiological processes in mammalian cells. Recent studies have demonstrated that H2 S promotes aerobic energy production in the mitochondria in response to hypoxia, but its effect on anaerobic energy production has yet to be established. Glycolysis is the anaerobic process by which ATP is produced through the metabolism of glucose. Mammalian red blood cells (RBCs) extrude mitochondria and nucleus during erythropoiesis. These cells would serve as a unique model to observe the effect of H2 S on glycolysis-mediated energy production. The purpose of this study was to determine the effect of H2 S on glycolysis-mediated energy production in mitochondria-free mouse RBCs. Western blot analysis showed that the only H2 S-generating enzyme expressed in mouse RBCs is 3-mercaptopyruvate sulfurtransferase (MST). Supplement of the substrate for MST stimulated, but the inhibition of the same suppressed, the endogenous production of H2 S. Both exogenously administered H2 S salt and MST-derived endogenous H2 S stimulated glycolysis-mediated ATP production. The effect of NaHS on ATP levels was not affected by oxygenation status. On the contrary, hypoxia increased intracellular H2 S levels and MST activity in mouse RBCs. The mitochondria-targeted H2 S donor, AP39, did not affect ATP levels of mouse RBCs. NaHS at low concentrations (3-100 μM) increased ATP levels and decreased cell viability after 3 days of incubation in vitro. Higher NaHS concentrations (300-1000 μM) lowered ATP levels, but prolonged cell viability. H2 S may offer a cytoprotective effect in mammalian RBCs to maintain oxygen-independent energy production.
- Published
- 2021
12. Improving sulforaphane content in transgenic broccoli plants by overexpressing MAM1, FMOGS–OX2, and Myrosinase
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Ruining Liu, Guangdong Yang, Jinhua Zhang, Haiyan Cao, Zhiqiang Liu, Yanxi Pei, Sanhong Fan, and Zhuping Jin
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0106 biological sciences ,biology ,Myrosinase ,Agrobacterium ,Transgene ,food and beverages ,Genetically modified crops ,Horticulture ,biology.organism_classification ,01 natural sciences ,Metabolic pathway ,chemistry.chemical_compound ,Transformation (genetics) ,Biochemistry ,chemistry ,Arabidopsis thaliana ,010606 plant biology & botany ,Sulforaphane - Abstract
Sulforaphane (SF) is a potential secondary metabolite with anticancer activity and its metabolic pathway has been revealed in the model plant Arabidopsis thaliana. Generally, the SF extraction cycle from broccoli is long and the yield is low, which means that it no longer meets cancer treatment requirements. Therefore, the SF content needs to be urgently improved. In this study, MAM1, FMOGS–OX2, and Myrosinase, which are required for SF biosynthesis, were introduced into broccoli by Agrobacterium tumefaciens-mediated transformation. To obtain plants with a higher SF content, broccoli was transformed using Myrosinase-FMOGS–OX2-MAM1 (M-F-A) triple genes in tandem and by adding each of the genes on their own. The results showed that the SF contents in the MAM1, FMOGS–OX2, or Myrosinase transgenic plants improved by 1.7–3.4, 1.6–2.7, and 3.7-fold compared to the wild type (WT), respectively, However, the SF contents in transgenic plants with all three genes improved by 1.86–5.5fold. Furthermore, we examined the anticancer effect of the SF extracts from transgenic plants via cell viability detection of HCT116 colon cancer cells. The results showed that SF extracts from transgenic plants had more obvious anticancer activity than SF extracts from WT, and the SF extracts from the multiple genes plants had stronger anticancer activity than the single gene plants. In summary, the multiple genes transformation increased the SF content in broccoli more than single gene introductions. The aim of this research was to improve sulforaphane content by introducing FMOGS–OX2, MAM1, and Myrosinase genes, and Myrosinase-FMOGS–OX2-MAM1 (M-F-A) serial triple genes into broccoli.
- Published
- 2021
13. Interaction among estrogen, IGF-1, and H2S on smooth muscle cell proliferation
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Zhongming Qian, Rui Wang, Lingyun Wu, Ying Huang, Ming Fu, Tian Shuang, and Guangdong Yang
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Male ,0301 basic medicine ,medicine.medical_specialty ,Vascular smooth muscle ,medicine.drug_class ,Endocrinology, Diabetes and Metabolism ,Myocytes, Smooth Muscle ,Estrogen receptor ,030209 endocrinology & metabolism ,Endogeny ,Receptor, IGF Type 1 ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Downregulation and upregulation ,Internal medicine ,medicine ,Animals ,Hydrogen Sulfide ,Protein Phosphatase 2 ,Insulin-Like Growth Factor I ,Receptor ,Aorta ,Cell Proliferation ,Mice, Knockout ,Gene knockdown ,Estradiol ,Cell growth ,Chemistry ,Estrogen Receptor alpha ,030104 developmental biology ,Estrogen ,cardiovascular system ,Female - Abstract
Both estrogen and hydrogen sulfide (H2S) inhibit the proliferation of vascular smooth muscle cells (SMCs) and development of atherosclerosis. In the absence of endogenous H2S as occurred in CSE-knockout (KO) mouse, however, estrogen stimulates the proliferation of vascular SMCs. The underlying mechanisms for this seemingly controversial vascular effect of estrogen are unclear. In the present study, we demonstrated that the stimulatory effect of estrogen on the proliferation of CSE-KO SMCs was suppressed by the inhibitor of insulin-like growth factor-1 receptor (IGF-1R) or knockdown of IGF-1R protein expression. Estrogen downregulated the expression of insulin-like growth factor-1 (IGF-1) and IGF-1R in aortic tissues or aortic SMCs isolated from WT and CSE-KO mice. Furthermore, endogenous H2S downregulated IGF-1R, but upregulated estrogen receptor (ER)-α, in aortic tissues or SMCs. ER-α and IGF-1R were co-located in SMCs and co-immunoprecipitated, which was decreased by H2S. Finally, both endogenous and exogenous H2S induced the S-sulfhydration of IGF-1R, but not ER-α, in WT-SMCs and CSE-KO SMCs, which underlies the decreased formation of IGF-1R/ER-α hybrid in the presence of H2S. Thus, the absence of H2S favors the interaction of estrogen with IGF-1R/ER-α hybrid to stimulate SMCs proliferation. The appreciation of a critical role of H2S in preventing estrogen-induced SMCs proliferation will help better understand the regulation of complex vascular effects of estrogen and sex-related cardiovascular diseases.
- Published
- 2021
14. Experimental and numerical investigation on dynamic behavior of RC orifice targets subjected to underwater explosions
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Guangdong Yang, Yong Fan, Gaohui Wang, Xianze Cui, Qi Li, and Zhendong Leng
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Civil and Structural Engineering - Published
- 2023
15. Mitigation effects of air-backed RC slabs retrofitted with CFRP subjected to underwater contact explosions
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Guangdong Yang, Yong Fan, Gaohui Wang, Xianze Cui, Qi Li, Zhendong Leng, and Ke Deng
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Environmental Engineering ,Ocean Engineering - Published
- 2023
16. Hydrogen sulfide and metal interaction: the pathophysiological implications
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Devin Mantle and Guangdong Yang
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Mammals ,Carbon Monoxide ,Gasotransmitters ,Clinical Biochemistry ,Animals ,Cell Biology ,General Medicine ,Hydrogen Sulfide ,Nitric Oxide ,Molecular Biology ,Signal Transduction - Abstract
Hydrogen sulfide (H
- Published
- 2022
17. Preparation and Properties of Foamed Cement for Lightweight Thermal Insulation with Ti-Extraction Blast Furnace Slag and Sulfoaluminate Cement by Chemical Foaming
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Lin Li, Tao Jiang, Bojian Chen, Jing Wen, and Guangdong Yang
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General Materials Science ,Building and Construction ,Civil and Structural Engineering - Published
- 2022
18. H2S as a potential defense against COVID-19?
- Author
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Guangdong Yang
- Subjects
0301 basic medicine ,Physiology ,business.industry ,Inflammation ,Cell Biology ,Pharmacology ,medicine.disease_cause ,Entry into host ,TMPRSS2 ,Transmembrane Protease Serine 2 ,Virus ,3. Good health ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Immune system ,030220 oncology & carcinogenesis ,medicine ,Signal transduction ,medicine.symptom ,business ,Coronavirus - Abstract
The outbreak of COVID-19 pneumonia caused by a new coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) is posing a global health emergency and has led to more than 380,000 deaths worldwide. The cell entry of SARS-CoV-2 depends on two host proteins angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2). There is currently no vaccine available and also no effective drug for the treatment of COVID-19. Hydrogen sulfide (H2S) as a novel gasotransmitter has been shown to protect against lung damage via its anti-inflammation, antioxidative stress, antiviral, prosurvival, and antiaging effects. In light of the research advances on H2S signaling in biology and medicine, this review proposed H2S as a potential defense against COVID-19. It is suggested that H2S may block SARS-CoV-2 entry into host cells by interfering with ACE2 and TMPRSS2, inhibit SARS-CoV-2 replication by attenuating virus assembly/release, and protect SARS-CoV-2-induced lung damage by suppressing immune response and inflammation development. Preclinical studies and clinical trials with slow-releasing H2S donor(s) or the activators of endogenous H2S-generating enzymes should be considered as a preventative treatment or therapy for COVID-19.
- Published
- 2020
19. Golgi Stress Response, Hydrogen Sulfide Metabolism, and Intracellular Calcium Homeostasis
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Lingyun Wu, Guangdong Yang, Ming Fu, Yanxi Pei, Ethan Read, Yuehong Wang, Rui Wang, and Yanjie Zhang
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Male ,0301 basic medicine ,Cell Survival ,Physiology ,Clinical Biochemistry ,Golgi Apparatus ,Apoptosis ,Sodium hydrosulfide ,Biochemistry ,Calcium in biology ,Mice ,03 medical and health sciences ,symbols.namesake ,chemistry.chemical_compound ,parasitic diseases ,medicine ,Animals ,Homeostasis ,Myocyte ,Hydrogen Sulfide ,Molecular Biology ,Cells, Cultured ,General Environmental Science ,Mice, Knockout ,030102 biochemistry & molecular biology ,Chemistry ,Skeletal muscle ,Cell Biology ,Golgi apparatus ,Brefeldin A ,equipment and supplies ,Angiotensin II ,Cell biology ,Oxidative Stress ,030104 developmental biology ,medicine.anatomical_structure ,symbols ,General Earth and Planetary Sciences ,Calcium ,Intracellular - Abstract
Aims: The physiological and pathological importance of hydrogen sulfide (H2S) as a novel gasotransmitter has been widely recognized. Cystathionine gamma-lyase (CSE) is one of the major H2S-producing enzymes and it regulates diverse functions in connection with intracellular calcium (Ca2+). The aim of this study is to examine the role of H2S in Golgi stress-related cell injury and skeletal muscle disorders. Results: Golgi stressors (brefeldin A [BFA] and monensin) decreased the expression of GM130 and ATP2C1 (two markers of Golgi stress response), induced Golgi apparatus fragmentation, and caused a higher level of oxidative stress and cell apoptosis in mouse myoblast cells. In addition, Golgi stressors upregulated CSE expression and endogenous H2S generation, and exogenously applied H2S was able to protect but inhibition of CSE/H2S system deteriorated Golgi stress response. Activating transcription factor 4 (ATF4) acted as an upstream molecule to increase CSE expression on Golgi stress response. Mechanically, Golgi stressors induced intracellular level of Ca2+, and chelating cellular Ca2+ markedly attenuated Golgi stress response, indicating the key role of Ca2+ in initiating Golgi stress and cell apoptosis. Further, administration of either angiotensin II or BFA initiated Golgi stress response and induced skeletal muscle atrophy in mice, which was further deteriorated by CSE deficiency but rescued by exogenously applied sodium hydrosulfide (NaHS). Innovation and Conclusion: The activation of the CSE/H2S pathway and the decrease of intracellular Ca2+ are two cellular protective mechanisms against Golgi stress, and the CSE/H2S system would be a target for preventing skeletal muscle dysfunctions.
- Published
- 2020
20. Failure investigation of a Kaplan turbine blade lever
- Author
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Jingwei Cao, Guangdong Yang, Yongyao Luo, Jie Chen, Run Liao, and Zhengwei Wang
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General Engineering ,General Materials Science - Published
- 2022
21. H
- Author
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Ruihuan, Yu, Yuehong, Wang, Jiechun, Zhu, and Guangdong, Yang
- Abstract
Hydrogen sulphide (H
- Published
- 2021
22. H
- Author
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Kalem, Barrow, Yuehong, Wang, Ruihuan, Yu, Jiechun, Zhu, and Guangdong, Yang
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Aging ,Mice ,Oxidative Stress ,Heart Diseases ,Cystathionine gamma-Lyase ,Animals ,Angiotensin-Converting Enzyme 2 ,Hydrogen Peroxide ,Hydrogen Sulfide ,Rats - Abstract
Cystathionine gamma-lyase (CSE)-derived hydrogen sulfide (H
- Published
- 2021
23. The interaction of disulfiram and H
- Author
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Ethan, Read, Jarod, Milford, Jiechun, Zhu, Lingyun, Wu, Marc, Bilodeau, and Guangdong, Yang
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Cell Survival ,Liver Neoplasms ,Acetaldehyde Dehydrogenase Inhibitors ,Temperature ,Antineoplastic Agents ,Aldehyde Dehydrogenase ,Hydrogen-Ion Concentration ,Mice ,Liver ,Cell Line, Tumor ,Disulfiram ,Animals ,Humans ,Hydrogen Sulfide ,Copper ,Alcohol Deterrents ,Cell Proliferation - Abstract
Disulfiram (DSF), a sulfur-containing compound, has been used to treat chronic alcoholism and cancer for decades by inactivating aldehyde dehydrogenase (ALDH). Hydrogen sulfide (H
- Published
- 2021
24. Cystathionine gamma‐lyase/H 2 S signaling facilitates myogenesis under aging and injury condition
- Author
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Amadeo M. Parissenti, Lingyun Wu, Yuehong Wang, Guangdong Yang, Simon J. Lees, Laura Masters, Baoqing Guo, Yanxi Pei, Yanjie Zhang, and Rui Wang
- Subjects
0301 basic medicine ,Cell signaling ,Chemistry ,Myogenesis ,Cellular differentiation ,Cystathionine gamma-lyase ,Skeletal muscle ,Biochemistry ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Genetics ,medicine ,Myocyte ,Molecular Biology ,C2C12 ,030217 neurology & neurosurgery ,Myogenin ,Biotechnology - Abstract
Hydrogen sulfide (H2 S) can be endogenously produced and belongs to the class of signaling molecules known as gasotransmitters. Cystathionine gamma-lyase (CSE)-derived H2 S is implicated in the regulation of cell differentiation and the aging process, but the involvements of the CSE/H2 S system in myogenesis upon aging and injury have not been explored. In this study, we demonstrated that CSE acts as a major H2 S-generating enzyme in skeletal muscles and is significantly down-regulated in aged skeletal muscles in mice. CSE deficiency exacerbated the age-dependent sarcopenia and cardiotoxin-induced injury/regeneration in mouse skeletal muscle, possibly attributed to inefficient myogenesis. In contrast, supplement of NaHS (an H2 S donor) induced the expressions of myogenic genes and promoted muscle regeneration in mice. In vitro, incubation of myoblast cells (C2C12) with H2 S promoted myogenesis, as evidenced by the inhibition of cell cycle progression and migration, altered expressions of myogenic markers, elongation of myoblasts, and formation of multinucleated myotubes. Myogenesis was also found to upregulate CSE expression, while blockage of CSE/H2 S signaling resulted in a suppression of myogenesis. Mechanically, H2 S significantly induced the heterodimer formation between MEF2c and MRF4 and promoted the binding of MEF2c/MRF4 to myogenin promoter. MEF2c was S-sulfhydrated at both cysteine 361 and 420 in the C-terminal transactivation domain, and blockage of MEF2c S-sulfhydration abolished the stimulatory role of H2 S on MEF2c/MRF4 heterodimer formation. These findings support an essential role for H2 S in maintaining myogenesis, presenting it as a potential candidate for the prevention of age-related sarcopenia and treatment of muscle injury.
- Published
- 2021
25. Induction of cystathionine gamma-lyase expression and metallothionein-1 S-sulfhydration alleviate cadmium-induced cell death in myoblast cells
- Author
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Amr Ali, Zhuping Jin, Guangdong Yang, Yanxi Pei, and Yanjie Zhang
- Subjects
Programmed cell death ,Cell Survival ,Health, Toxicology and Mutagenesis ,Apoptosis ,Sulfides ,medicine.disease_cause ,Metal regulatory transcription factor 1 ,Cell Line ,Myoblasts ,Mice ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Humans ,Metallothionein ,Myocyte ,Hydrogen Sulfide ,Sulfhydryl Compounds ,030304 developmental biology ,0303 health sciences ,biology ,Chemistry ,Cystathionine gamma-lyase ,Cystathionine gamma-Lyase ,Public Health, Environmental and Occupational Health ,General Medicine ,equipment and supplies ,Pollution ,Cystathionine beta synthase ,Cell biology ,Oxidative Stress ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,biology.protein ,Environmental Pollutants ,Intracellular ,Oxidative stress ,Cadmium - Abstract
Hydrogen sulfide (H2S), a multifunctional gasotransmitter, participates in a wide range of cellular signal transduction and pathophysiological processes. Cystathionine gamma-lyase (CSE) acts as a major H2S-generating enzyme in peripheral organs and tissues. As a cysteine-rich and heavy metal-binding protein, metallothionein-1 (MT-1) is known to protect cells from various environmental stresses. Here we demonstrated that exposure of cadmium (Cd) induced oxidative stress, depleted intracellular thiols, and stimulated apoptotic cell death in mouse myoblast cells. CSE expression and H2S production were significantly enhanced by Cd treatment. NaHS, a well-known H2S donor, at physiologically relevant concentration significantly alleviated Cd-induced damage in both myoblasts and mouse skeletal muscles. In contrast, down-regulation of CSE/H2S system deteriorated Cd-stimulated oxidative stress and cell death. Exposure of the cells to Cd lead to increased expressions of metal regulatory transcription factor 1 and MT-1, while siRNA-mediated MT-1 knockdown alleviated Cd-induced CSE expression and caused more oxidative stress and cell death. In addition, H2S post-translationally modified MT-1 by S-sulfhydration and stabilized zinc-protein complex. Taken together, these data suggest that CSE/H2S system would protect myoblasts and skeletal muscles from Cd-induced damage by S-sufhydrating MT-1.
- Published
- 2019
26. Hydrogen Sulfide
- Author
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Yanxi Pei, Guangdong Yang, and Yanjie Zhang
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chemistry.chemical_compound ,Chemistry ,Hydrogen sulfide ,Plant defense against herbivory ,Combinatorial chemistry - Published
- 2019
27. Experimental and numerical study of damage characteristics of RC slabs subjected to air and underwater contact explosions
- Author
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Gaohui Wang, Peng Yan, Guangdong Yang, Ming Chen, Liang Wu, and Wenbo Lu
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Materials science ,business.industry ,Mechanical Engineering ,Bubble ,0211 other engineering and technologies ,Detonation ,020101 civil engineering ,Ocean Engineering ,02 engineering and technology ,Structural engineering ,Numerical models ,Reinforced concrete ,0201 civil engineering ,Mechanics of Materials ,General Materials Science ,Underwater ,business ,021101 geological & geomatics engineering - Abstract
Reinforced concrete (RC) is one of the most commonly used construction materials. However, impact and blast loads could completely destroy RC structures, causing tremendous casualties and property losses. Therefore, analyzing the damage mechanism and dynamic behaviour of RC structures under blast loading is significant. This paper presents a study on the behaviour of RC slabs subjected to air and underwater contact detonation. Two RC slabs were tested under real blast loads. One was tested under air contact explosion of 210 g TNT, the other was tested subjected to underwater contact explosion of 6 g TNT. Damage evolution and damage mechanism of the RC slabs subjected to the air and underwater contact explosions are investigated by using high-fidelity numerical models. The damage patterns obtained from the numerical models match well with those of the tests. The effect of experimental environments on the structural behaviour of the slabs subjected to the underwater explosions is also discussed. The different response of the RC slabs subjected air and underwater explosions with identical charge mass are analyzed contrastively by employing the validated coupled method. The results show that the present model can give a reliable prediction of damage characteristics for RC structures under various blast scenarios. The experimental environments have important influence on the structural response and bubble development. The damage of the RC slabs induced by underwater contact explosion is significantly larger than that caused by air contact blast with equal charge mass.
- Published
- 2019
28. Cross-section shape effects on anti-knock performance of RC columns subjected to air and underwater explosions
- Author
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Gaohui Wang, Ming Chen, Peng Yan, Guangdong Yang, Xiaohua Zhao, and Wenbo Lu
- Subjects
Environmental Engineering ,Materials science ,Mathematical model ,business.industry ,020101 civil engineering ,Ocean Engineering ,02 engineering and technology ,Structural engineering ,Underwater blast ,01 natural sciences ,Rc columns ,010305 fluids & plasmas ,0201 civil engineering ,Cross section (physics) ,Nonlinear system ,Column (typography) ,0103 physical sciences ,Underwater ,business ,Underwater explosion - Abstract
Columns are the key load-bearing elements in infrastructures. Common cross-section shapes of columns are square and circle. Those columns may be subjected to various blasting scenarios. This paper investigates the effects of cross-section shape on the anti-knock performance of RC columns subjected to air and underwater blast loads. The reliability of the fully coupled Lagrangian-Eulerian numerical model is verified by comparing with the results of the field blast test. The damage characteristics and dynamic response of the RC column with different cross-section shapes subjected to contact and close-in air explosions are investigated. The anti-knock performance of the RC column with different cross-section shapes subjected to contact and close-in underwater explosion is also studied. Nonlinear dynamic response behavior and damage mechanism of the RC column subjected to contact and close-in explosion are compared. The improvement effects of the reinforcement ratio and ultra-high performance concrete on the blast resistance of the underwater RC column are investigated. The results show that the cross-section shape has an important influence on the anti-knock performance of the column, whether subjected to air or underwater explosions. Employing circular cross-section can effectively improve the blast resistance of the column.
- Published
- 2019
29. Numerical study on dynamic response of submerged tunnel subjected to underwater explosion
- Author
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Guangdong Yang, Yong Fan, Gaohui Wang, Qidong Gao, Ke Deng, and Fei Wang
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Soil Science ,Geotechnical Engineering and Engineering Geology ,Civil and Structural Engineering - Published
- 2022
30. Hydrogen sulfide and hepatic lipid metabolism – a critical pairing for liver health
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Guangdong Yang, Lingyun Wu, and Julie J. Loiselle
- Subjects
0301 basic medicine ,Hydrogen sulfide ,Bioinformatics ,03 medical and health sciences ,chemistry.chemical_compound ,Liver disease ,0302 clinical medicine ,Diabetes mellitus ,Animals ,Medicine ,Hydrogen Sulfide ,Pharmacology ,Gasotransmitters ,business.industry ,Lipid metabolism ,Metabolism ,Lipid Metabolism ,medicine.disease ,Lipids ,3. Good health ,Metabolic pathway ,030104 developmental biology ,Liver ,chemistry ,Hepatic lipid ,Knockout animal ,business ,030217 neurology & neurosurgery ,Themed Section: Review Article - Abstract
Hydrogen sulfide (H(2)S) is the most recently recognized gasotransmitter, influencing a wide range of physiological processes. As a critical regulator of metabolism, H(2)S has been suggested to be involved in the pathology of many diseases, particularly obesity, diabetes and cardiovascular disorders. Its involvement in liver health has been brought to light more recently, particularly through knockout animal models, which show severe hepatic lipid accumulation upon ablation of H(2)S metabolic pathways. A complex relationship between H(2)S and lipid metabolism in the liver is emerging, which has significant implications for liver disease establishment and/or progression, regardless of the disease‐causing agent. In this review, we discuss the critical importance of H(2)S in hepatic lipid metabolism. We then describe the animal models so far related with H(2)S and lipid‐associated liver disease, as well as H(2)S‐based treatments available. Finally, we highlight important considerations for future studies and identify areas in which much still remains to be determined. LINKED ARTICLES: This article is part of a themed section on Hydrogen Sulfide in Biology & Medicine. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v177.4/issuetoc
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- 2018
31. Numerical modeling of surface explosion effects on shallow-buried box culvert behavior during the water diversion
- Author
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Gaohui Wang, Guangdong Yang, Xiaohua Zhao, Wenbo Lu, Peng Yan, and Ming Chen
- Subjects
Shock wave ,Surface (mathematics) ,Soil model ,Culvert ,Mechanical Engineering ,Flow (psychology) ,0211 other engineering and technologies ,Numerical modeling ,020101 civil engineering ,02 engineering and technology ,Building and Construction ,Reinforced concrete ,0201 civil engineering ,Water diversion ,Environmental science ,Geotechnical engineering ,021101 geological & geomatics engineering ,Civil and Structural Engineering - Abstract
This paper is aim to investigate the damage characteristics of a shallow-buried water diversion box culvert subjected to a surface explosion. For this purpose, the soil model is verified by comparing with the analytical formulas of the Technical Manual (TM5-855-1), and the accuracy of the reinforced concrete model is validated with a previous explosion test. Subsequently, a three-dimensional (3D) numerical model of the water diversion culvert is established. A fully coupled Eulerian and Lagrangian (CEL) approach is presented to model the soil-structure-water interaction. The shock wave propagation characteristics in the soil, culvert and water medium are captured. The dynamic responses and damage processes of the shallow-buried box culvert in design water diversion condition (i.e. unconfined full flow) subjected to surface explosion are investigated. Other three water diversion conditions (i.e. pressure flow, free flow and no water) are employed to evaluate the influence of the water diversion condition on the antiknock performance of the culvert. The effects of the burial depth and charge weight on the dynamic behavior of the culvert to surface explosion are also discussed. The results show that the surface blast loads will cause a great threat to the whole security of the shallow-buried water diversion box culvert. The water diversion condition has a significant influence on the dynamic behavior of the culvert when subjected to a surface explosion.
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- 2018
32. H2S is involved in ABA-mediated stomatal movement through MPK4 to alleviate drought stress in Arabidopsis thaliana
- Author
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Liping Zhang, Xin Liu, Xinzhe Du, Guangdong Yang, Zhuping Jin, and Yanxi Pei
- Subjects
0106 biological sciences ,MAPK/ERK pathway ,biology ,Kinase ,fungi ,Mutant ,food and beverages ,Soil Science ,Plant physiology ,04 agricultural and veterinary sciences ,Plant Science ,equipment and supplies ,biology.organism_classification ,01 natural sciences ,Cell biology ,chemistry.chemical_compound ,chemistry ,Arabidopsis ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Arabidopsis thaliana ,Protein kinase A ,Abscisic acid ,010606 plant biology & botany - Abstract
Hydrogen sulfide (H2S) is a gaseous signaling molecule that participates in multiple physiological processes in both animals and plants. Mitogen-activated protein kinase (MAPK) is important signaling molecule that links the growth and developmental signals and environment stimuli to cellular responses. In the current study we explored the relationship between H2S and MAPK in drought stress resistance in Arabidopsis. The quantitative real-time (qRT)-PCR, root tip bending experiment and stomatal aperture assay were used in this paper. Drought stress activated both H2S biosynthesis and gene expression of MAPKs. The increase in MAPK expression was depressed in lcd/des1, a double mutant of H2S synthesis. Then we selected MPK4 as our target and used mpk4 mutants for further studies. H2S was able to alleviate the drought stress in wild-type (WT) Arabidopsis but not in mpk4 mutants. Meanwhile, H2S-induced stomatal movement was impaired in mpk4 mutants. We then examined the role of H2S and MPK4 in stomatal movements in response to abscisic acid (ABA) and hydrogen peroxide (H2O2). ABA- and H2O2- mediated stomatal movements were impaired in lcd/des1 and mpk4 mutants, and H2S-induced stomatal closure was impaired in slac1–3 mutants. Our results suggested that MPK4 is important downstream of H2S in the drought stress response and in stomatal movement, and that the H2S-MPK4 cascade is involved in ABA-mediated stomatal movement to regulate the drought stress.
- Published
- 2018
33. Efflux inhibition by H2S confers sensitivity to doxorubicin-induced cell death in liver cancer cells
- Author
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Tian Shuang, Guangdong Yang, Amadeo M. Parissenti, Eric Stokes, Lingyun Wu, Ming Fu, Baoqing Guo, Yanjie Zhang, Rui Wang, and Yanxi Pei
- Subjects
0301 basic medicine ,Programmed cell death ,Chemistry ,General Medicine ,equipment and supplies ,General Biochemistry, Genetics and Molecular Biology ,3. Good health ,Blot ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,Annexin ,030220 oncology & carcinogenesis ,Cancer cell ,polycyclic compounds ,medicine ,Cancer research ,Doxorubicin ,Propidium iodide ,General Pharmacology, Toxicology and Pharmaceutics ,Liver X receptor ,Clonogenic assay ,medicine.drug - Abstract
Aims Hydrogen sulfide (H2S), an important gasotransmitter, is involved in a variety of cellular functions and pathophysiologic processes. Drug resistance due to alterations in drug trafficking and metabolism severely limits the effectiveness of cancer therapy. This study examined the role of H2S in drug resistance in liver cancer cells. Materials and methods Human primary hepatocellular carcinoma cell line (HepG2) and doxorubicin (Dox)-resistant cells were used in this study. Cell survival was analyzed by MTT, Annexin V-FITC/propidium iodide staining and clonogenic assay. Western blotting was used for analysis of protein expression, and immunoprecipitation was used to determine interactions of LXR/RXR. Key findings The expression of H2S-generating enzyme cystathionine gamma-lyase (CSE) was inhibited by doxorubicin treatment in HepG2 cells, and H2S sensitized Dox-inhibited cell survival and colony formation. In addition, H2S promoted cellular retention of Dox by suppressing the expressions of ABCA1 and ABCG8. H2S significantly blocked Dox-induced heterodimer formation between LXRα and RXRβ and attenuated the binding of LXRα/RXRβ to the promoters of ABCA1 and ABCG8 genes. RXRβ but not LXRα was S-sulfhydrated by H2S, and blockage of RXRβ S-sulfhydration abolished the inhibitory role of H2S on LXRα/RXRβ heterodimer formation. CSE expression was reduced in Dox-resistant cells in comparison with their parental cells, while H2S could reverse drug resistance in Dox-resistant cells. Significance Our study provides a novel solution for reversing drug resistance in cancer cells by targeting H2S signalling.
- Published
- 2018
34. Experimental investigation of RC slabs under air and underwater contact explosions
- Author
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Ming Chen, Xiaohua Zhao, Wenbo Lu, Gaohui Wang, Peng Yan, and Guangdong Yang
- Subjects
Environmental Engineering ,Materials science ,business.industry ,0211 other engineering and technologies ,02 engineering and technology ,Structural engineering ,Reinforced concrete ,Reinforced concrete slab ,021105 building & construction ,Underwater ,business ,Failure mode and effects analysis ,021101 geological & geomatics engineering ,Civil and Structural Engineering - Abstract
The blast experimental test is an effective method to observe the failure characteristics of reinforced concrete (RC) slabs to contact explosion. However, the existing explosion experiments on RC s...
- Published
- 2018
35. Reversal of Sp1 transactivation and TGFβ1/SMAD1 signaling by H2S prevent nickel-induced fibroblast activation
- Author
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Tian Shuang, Yanjie Zhang, Guangdong Yang, Lingyun Wu, Rui Wang, Yanxi Pei, Ming Fu, and Mélanie Racine
- Subjects
inorganic chemicals ,0301 basic medicine ,Pharmacology ,biology ,Cell growth ,Chemistry ,Cell migration ,equipment and supplies ,Toxicology ,Cell biology ,Fibronectin ,03 medical and health sciences ,Transactivation ,030104 developmental biology ,medicine.anatomical_structure ,otorhinolaryngologic diseases ,medicine ,biology.protein ,Viability assay ,Cell activation ,Fibroblast ,Intracellular - Abstract
Nickel as a heavy metal is known to bring threat to human health, and nickel exposure is associated with changes in fibroblast activation which may contribute to its fibrotic properties. H2S has recently emerged as an important gasotransmitter involved in numerous cellular signal transduction and pathophysiological responses. Interaction of nickel and H2S on fibroblast cell activation has not been studied so far. Here, we showed that a lower dose of nickel (200 μM) induced the activation of human fibroblast cells, as evidenced by increased cell growth, migration and higher expressions of α-smooth muscle actin (αSMA) and fibronectin, while high dose of nickel (1 mM) inhibited cell viability. Nickel reduced intracellular thiol contents and stimulated oxidative stress. Nickel also repressed the mRNA and protein expression of cystathionine gamma-lyase (CSE, a H2S-generating gene) and blocked the endogenous production of H2S. Exogenously applied NaHS (a H2S donor) had no effect on nickel-induced cell viability but significantly attenuated nickel-stimulated cell migration and the expression of αSMA and fibronectin. In contrast, CSE deficiency worsened nickel-induced αSMA expression. Moreover, H2S incubation reversed nickel-stimulated TGFβ1/SMAD1 signal and blocked TGFβ1-initiated expressions of αSMA and fibronectin. Nickel inhibited the interaction of Sp1 with CSE promoter but strengthened the binding of Sp1 with TGFβ1 promoter, which was reversed by exogenously applied NaHS. These data reveal that H2S protects from nickel-stimulated fibroblast activation and CSE/H2S system can be a potential target for the treatment of tissue fibrosis induced by nickel.
- Published
- 2018
36. H2S Persulfidated and Increased Kinase Activity of MPK4 to Response Cold Stress in Arabidopsis
- Author
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Xinzhe Du, Zhuping Jin, Zhiqiang Liu, Danmei Liu, Liping Zhang, Xiaoli Ma, Guangdong Yang, Sha Liu, Yarong Guo, and Yanxi Pei
- Subjects
0106 biological sciences ,0301 basic medicine ,Activity level ,MPK4 ,Hydrogen sulfide ,Mutant ,hydrogen sulfide ,01 natural sciences ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Biochemistry ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,Arabidopsis ,Molecular Biosciences ,Kinase activity ,lcsh:QH301-705.5 ,Molecular Biology ,MEK2 ,Original Research ,biology ,Kinase ,Abiotic stress ,equipment and supplies ,biology.organism_classification ,Cell biology ,030104 developmental biology ,lcsh:Biology (General) ,chemistry ,cold stress ,Persulfidation ,010606 plant biology & botany - Abstract
Hydrogen sulfide (H2S) is a gasotransmitter along with nitric oxide and carbon oxide, which is involved in plant growth and development as well as biotic and abiotic stress resistance. In a previous study, we reported that mitogen-activated protein kinases, especially MPK4, are important downstream components of H2S involved in alleviating cold stress; however the underlying mechanism is unclear. In this study, we determined that the ability of H2S to alleviate cold stress is impaired in mpk4 mutants, but not in the upstream mek2 and crlk1 mutants. MPK4 was basically persulfidated, and NaHS (H2S donor) further increased the persulfidation level of MPK4. MEK2 was not persulfidated by H2S. NaHS treatments increased the MPK4 activity level nearly tenfold. The persulfidation signal of MPK4 did not disappear after eight cystein residues in MPK4 were site-mutated, respectively. Above all, our results suggested that H2S alleviates cold stress directly by persulfidating MPK4 and increasing the MPK4 kinase activity.
- Published
- 2021
37. Cystathionine gamma-lyase/H
- Author
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Yanjie, Zhang, Laura, Masters, Yuehong, Wang, Lingyun, Wu, Yanxi, Pei, Baoqing, Guo, Amadeo, Parissenti, Simon J, Lees, Rui, Wang, and Guangdong, Yang
- Subjects
Male ,Myoblasts ,Aging ,Mice ,Sarcopenia ,Cystathionine gamma-Lyase ,Animals ,Cell Differentiation ,Hydrogen Sulfide ,Muscle Development ,Muscle, Skeletal - Abstract
Hydrogen sulfide (H
- Published
- 2021
38. Disrupted H2S Signaling by Cigarette Smoking and Alcohol Drinking: Evidence from Cellular, Animal, and Clinical Studies
- Author
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Jiechun Zhu, Guangdong Yang, and Ethan Read
- Subjects
0301 basic medicine ,Antioxidant ,Physiology ,Chronic oxidative stress ,medicine.medical_treatment ,Clinical Biochemistry ,Regulator ,cigarette smoking ,Alcohol ,Inflammation ,medicine.disease_cause ,Bioinformatics ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cigarette smoking ,medicine ,oxidative stress ,Molecular Biology ,business.industry ,H2S ,lcsh:RM1-950 ,Cell Biology ,equipment and supplies ,alcohol drinking ,3. Good health ,Review article ,030104 developmental biology ,lcsh:Therapeutics. Pharmacology ,chemistry ,inflammation ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Oxidative stress - Abstract
The role of endogenous hydrogen sulfide (H2S) as an antioxidant regulator has sparked interest in its function within inflammatory diseases. Cigarette and alcohol use are major causes of premature death, resulting from chronic oxidative stress and subsequent tissue damage. The activation of the Nrf2 antioxidant response by H2S suggests that this novel gasotransmitter may function to prevent or potentially reverse disease progression caused by cigarette smoking or alcohol use. The purpose of this study is to review the interrelationship between H2S signaling and cigarette smoking or alcohol drinking. Based on the databases of cellular, animal, and clinical studies from Pubmed using the keywords of H2S, smoking, and/or alcohol, this review article provides a comprehensive insight into disrupted H2S signaling by alcohol drinking and cigarette smoking-caused disorders. Major signaling and metabolic pathways involved in H2S-derived antioxidant and anti-inflammatory responses are further reviewed. H2S supplementation may prove to be an invaluable asset in treating or preventing diseases in those suffering from cigarette or alcohol addiction.
- Published
- 2021
39. Gasotransmitter signaling in energy homeostasis and metabolic disorders
- Author
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Amr Ali, Guangdong Yang, Yuehong Wang, and Lingyun Wu
- Subjects
0301 basic medicine ,030102 biochemistry & molecular biology ,Gasotransmitters ,Hydrogen sulfide ,food and beverages ,General Medicine ,equipment and supplies ,Biochemistry ,Small molecule ,Energy homeostasis ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Metabolic Diseases ,Biophysics ,Homeostasis ,Humans ,Oxidation-Reduction ,Carbon monoxide ,Signal Transduction - Abstract
Gasotransmitters are small molecules of gases, including nitric oxide (NO), hydrogen sulfide (H2S), and carbon monoxide (CO). These three gasotransmitters can be endogenously produced and regulate ...
- Published
- 2020
40. Disrupted H
- Author
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Ethan, Read, Jiechun, Zhu, and Guangdong, Yang
- Subjects
H2S ,inflammation ,cigarette smoking ,oxidative stress ,Review ,equipment and supplies ,alcohol drinking - Abstract
The role of endogenous hydrogen sulfide (H2S) as an antioxidant regulator has sparked interest in its function within inflammatory diseases. Cigarette and alcohol use are major causes of premature death, resulting from chronic oxidative stress and subsequent tissue damage. The activation of the Nrf2 antioxidant response by H2S suggests that this novel gasotransmitter may function to prevent or potentially reverse disease progression caused by cigarette smoking or alcohol use. The purpose of this study is to review the interrelationship between H2S signaling and cigarette smoking or alcohol drinking. Based on the databases of cellular, animal, and clinical studies from Pubmed using the keywords of H2S, smoking, and/or alcohol, this review article provides a comprehensive insight into disrupted H2S signaling by alcohol drinking and cigarette smoking-caused disorders. Major signaling and metabolic pathways involved in H2S-derived antioxidant and anti-inflammatory responses are further reviewed. H2S supplementation may prove to be an invaluable asset in treating or preventing diseases in those suffering from cigarette or alcohol addiction.
- Published
- 2020
41. Spermine Protects Cardiomyocytes from High Glucose-Induced Energy Disturbance by Targeting the CaSR-gp78-Ubiquitin Proteasome System
- Author
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Guangdong Yang, Hongzhu Li, Yuwen Wang, Changqing Xu, Can Wei, Xinying Zhang, Yuehong Wang, and Fadong Li
- Subjects
0301 basic medicine ,FIS1 ,Proteasome Endopeptidase Complex ,Diabetic Cardiomyopathies ,MFN2 ,Cell Culture Techniques ,Spermine ,030204 cardiovascular system & hematology ,Ornithine decarboxylase ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Downregulation and upregulation ,MFN1 ,Medicine ,Animals ,Pharmacology (medical) ,Myocytes, Cardiac ,Rats, Wistar ,Pharmacology ,business.industry ,Ubiquitin ,Respiratory chain complex ,General Medicine ,Cell biology ,Mitochondria ,Rats ,030104 developmental biology ,Glucose ,chemistry ,Cardiology and Cardiovascular Medicine ,business ,Energy Metabolism ,Receptors, Calcium-Sensing ,Intracellular - Abstract
To determine the mediation of spermine on energy metabolism disorder and diabetic cardiomyopathy (DCM) development as well as the underlying mechanisms. An in vitro model of DCM was established by incubating primary cultured neonatal rat cardiomyocytes with high glucose (HG). Spermine content was assessed by RP-HPLC. The protein levels were detected by western blot. Mitochondrial functions were analyzed using the respiratory chain complex assay kit and immunofluorescence staining. The endogenous content of spermine was decreased in the HG group, and the protein levels of ornithine decarboxylase, respiratory chain complex (I–V), mitochondrial fusion-related protein (Mfn1, Mfn2), Cx43, N-cadherin, CaSR, and β-catenin (in cytomembrane) were also down-regulated by HG. In contrast, the protein levels of spermine-N1-acetyltransferase, gp78, Fis1, Drp1, and β-catenin were up-regulated by HG. Meanwhile, we observed that HG increased ubiquitination levels of Mfn1, Mfn2, and Cx43, decreased membrane potential (ΔΨm), and the opening of mitochondrial permeability transport pore (mPTP) followed by intracellular ATP leakage. The supplement of spermine or siRNA-mediated knockdown of gp78 significantly alleviated the detrimental effects of HG, while downregulation of CaSR aggravated the development of DCM. We further confirmed that the lower level of spermine by HG activates the gp78-ubiquitin-proteasome pathway via downregulation of CaSR protein level, which in turn damages mitochondrial gap junction intercellular communication and leads to reduced ATP level. The protective role of spermine on energy metabolism disorder is based on higher CaSR protein level and lower gp78 activation, pointing to the possibility that spermine can be a target for the prevention and treatment of DCM.
- Published
- 2020
42. H
- Author
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Guangdong, Yang
- Subjects
SARS-CoV-2 ,H2S ,Pneumonia, Viral ,Serine Endopeptidases ,COVID-19 ,ACE2 ,Peptidyl-Dipeptidase A ,Virus Internalization ,Mini-Review ,Virus Replication ,Antiviral Agents ,COVID-19 Drug Treatment ,Betacoronavirus ,Host-Pathogen Interactions ,Animals ,Humans ,Angiotensin-Converting Enzyme 2 ,Hydrogen Sulfide ,Coronavirus Infections ,Lung ,Pandemics ,TMPRSS2 ,Signal Transduction - Abstract
The outbreak of COVID-19 pneumonia caused by a new coronavirus (severe acute respiratory syndrome coronavirus 2, SARS-CoV-2) is posing a global health emergency and has led to more than 380,000 deaths worldwide. The cell entry of SARS-CoV-2 depends on two host proteins angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2). There is currently no vaccine available and also no effective drug for the treatment of COVID-19. Hydrogen sulfide (H(2)S) as a novel gasotransmitter has been shown to protect against lung damage via its anti-inflammation, antioxidative stress, antiviral, prosurvival, and antiaging effects. In light of the research advances on H(2)S signaling in biology and medicine, this review proposed H(2)S as a potential defense against COVID-19. It is suggested that H(2)S may block SARS-CoV-2 entry into host cells by interfering with ACE2 and TMPRSS2, inhibit SARS-CoV-2 replication by attenuating virus assembly/release, and protect SARS-CoV-2-induced lung damage by suppressing immune response and inflammation development. Preclinical studies and clinical trials with slow-releasing H(2)S donor(s) or the activators of endogenous H(2)S-generating enzymes should be considered as a preventative treatment or therapy for COVID-19.
- Published
- 2020
43. Hydrogen sulfide signaling in regulation of cell behaviors
- Author
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Lingyun Wu, Ruihuan Yu, Yuehong Wang, and Guangdong Yang
- Subjects
0301 basic medicine ,Senescence ,Cancer Research ,Programmed cell death ,Physiology ,Cell Survival ,Cellular differentiation ,Clinical Biochemistry ,Cell ,030204 cardiovascular system & hematology ,Biology ,Biochemistry ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Humans ,Epigenetics ,Hydrogen Sulfide ,Gene ,Cellular Senescence ,Cell Death ,Cell Differentiation ,equipment and supplies ,3. Good health ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Histone ,DNA methylation ,biology.protein ,Signal Transduction - Abstract
Recent advances in the biomedical importance of H2S have help us understand various cellular functions and pathophysiological processes from a new aspect. Specially, H2S has been demonstrated to play multiple roles in regulating cell behaviors, including cell survival, cell differentiation, cell senescence, cell hypertrophy, cell atrophy, cell metaplasia, and cell death, etc. H2S contributes to cell behavior changes via various mechanisms, such as histone modification, DNA methylation, non-coding RNA changes, DNA damage repair, transcription factor activity, and post-translational modification of proteins by S-sulfhydration, etc. In this review, we summarized the recent research progress on H2S signaling in control of cell behaviors and discussed the ways of H2S regulation of gene expressions. Given the key roles of H2S in both health and diseases, a better understanding of the regulation of H2S on cell behavior change and the underlying molecular mechanisms will help us to develop novel and more effective strategies for clinical therapy.
- Published
- 2020
44. Cystathionine gamma-lyase/H
- Author
-
Amr, Ali, Yanjie, Zhang, Ming, Fu, Yanxi, Pei, Lingyun, Wu, Rui, Wang, and Guangdong, Yang
- Subjects
Male ,Mice, Knockout ,Cystathionine gamma-Lyase ,Hep G2 Cells ,Fatty Acids, Nonesterified ,Lipid Metabolism ,Mice, Inbred C57BL ,Disease Models, Animal ,Mice ,Acetyl Coenzyme A ,Non-alcoholic Fatty Liver Disease ,Animals ,Humans ,Hydrogen Sulfide - Abstract
Hydrogen sulfide (HBoth in vitro cell model and in vivo animal model of lipid accumulation were used in this study. Western blotting and real-time PCR were used for analysis of protein and mRNA expression. Acetyl-CoA was analyzed by a coupled enzyme assay, and lipid accumulation was observed with Oil Red O staining.Incubation of human liver carcinoma (HepG2) cells with a mixture of free fatty acids (FFAs) or high glucose reduced CSE expression and HCSE/H
- Published
- 2020
45. Combined effects of penetration and explosion on damage characteristics of a mass concrete target
- Author
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Guangdong Yang, Peng Yan, Ming Chen, Gaohui Wang, and Wenbo Lu
- Subjects
Mass concrete ,business.industry ,lcsh:Mechanical engineering and machinery ,Mechanical Engineering ,high strength concrete material ,SPH-Lagrange ,020101 civil engineering ,02 engineering and technology ,Structural engineering ,Penetration (firestop) ,0201 civil engineering ,020303 mechanical engineering & transports ,mass concrete target ,0203 mechanical engineering ,Environmental science ,Gravity dam ,lcsh:TJ1-1570 ,General Materials Science ,combined effects of penetration and explosion ,damage characteristics ,business ,High strength concrete - Abstract
With the development of the precision guidance technology, the earth-penetrating weapon (EPW) is a huge threat to infrastructures. The objective of this research is to investigate the damage characteristics of mass concrete targets under the combined action of penetration and explosion. The validity of the penetration model is discussed by reproducing a previous experimental test reported in the literature. Meanwhile, a field test about the internal explosion in a concrete cube is conducted to verify the validity of the internal explosion model. Subsequently, damage characteristics of a mass concrete target from the upper part of a concrete gravity dam are discussed when subjected to the combined action of the penetration and explosion. In order to improve the structural performance of the mass concrete target to penetration and explosion loadings, high strength concrete material is selected as a preventive measure. Penetration processes and damage patterns of the mass target with normal and high strength concrete material are compared. Then, three internal explosion models are presented to investigate the influence of the initial penetration damage on the failure characteristics of the mass concrete target. The results show that the resistance of the mass target to the combined action of the penetration and explosion can be enhanced significantly by using the high strength concrete material. The initial penetration damage has a significant influence on the damage processes of the mass concrete target subjected to internal blast loading.
- Published
- 2018
46. The interaction of IGF-1/IGF-1R and hydrogen sulfide on the proliferation of mouse primary vascular smooth muscle cells
- Author
-
Tian Shuang, Ming Fu, Lingyun Wu, Guangdong Yang, and Rui Wang
- Subjects
0301 basic medicine ,Vascular smooth muscle ,Myocytes, Smooth Muscle ,Sodium hydrosulfide ,Biochemistry ,Muscle, Smooth, Vascular ,Receptor, IGF Type 1 ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Western blot ,medicine ,Animals ,Hydrogen Sulfide ,Insulin-Like Growth Factor I ,Receptor ,Mesenteric arteries ,Cells, Cultured ,Cell Proliferation ,Podophyllotoxin ,Mice, Knockout ,Pharmacology ,medicine.diagnostic_test ,Cell growth ,Cystathionine gamma-Lyase ,3. Good health ,Cell biology ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,chemistry ,cardiovascular system ,RNA ,Picropodophyllin ,Bromodeoxyuridine - Abstract
Hydrogen sulfide (H2S) is mostly produced by cystathionine-gamma-lyase (CSE) in vascular system and it inhibits the proliferation of vascular smooth muscle cells (SMCs). Insulin-like growth factor-1 (IGF-1), via its receptor (IGF-1R), exerts multiple physiological and pathophysiological effects on the vasculature, including stimulating SMC proliferation and migration, and inhibiting SMC apoptosis. Since H2S and IGF-1/IGF-1R have opposite effects on SMC proliferation, it becomes imperative to better understand the interaction of these two signaling mechanisms on SMC proliferation. SMCs isolated from small mesenteric arteries of CSE knockout (KO) and wild-type (WT) mice were used in the present study. The effects of IGF-1 and H2S on SMC proliferation were evaluated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and bromodeoxyuridine (BrdU) assays. Protein expression was determined by western blot, and H2S-induced protein S-sulfhydration was assessed with a modified biotin switch assay. We found that IGF-1 dose-dependently increased the proliferation of both WT-SMCs and KO-SMCs, and this effect was more significant in KO-SMCs. Supplement of sodium hydrosulfide (NaHS) inhibited IGF-1-induced cell proliferation, while this effect was abolished by blocking IGF-1/IGF-1R signaling with picropodophyllin (PPP) or knocking out of the expression of IGF-1R. H2S significantly down-regulates the expression of IGF-1R, stimulates IGF-1R S-sulfhydration, and attenuates the binding of IGF-1 with IGF-1R. This study provides novel insight on the involvement of IGF-1/IGF-1R in H2S-inhibited SMC proliferation and suggests H2S-based innovative treatment strategies for proliferative cardiovascular diseases such as atherosclerosis.
- Published
- 2018
47. H2S protects lipopolysaccharide-induced inflammation by blocking NFκB transactivation in endothelial cells
- Author
-
Caitlyn Bourque, Adam Greasley, Ming Fu, Lingyun Wu, Yanjie Zhang, Mélanie Racine, Guangdong Yang, Yanxi Pei, and Rui Wang
- Subjects
0301 basic medicine ,Pharmacology ,Lipopolysaccharide ,Cell ,Inflammation ,Transfection ,equipment and supplies ,Toxicology ,3. Good health ,Cell biology ,03 medical and health sciences ,Transactivation ,chemistry.chemical_compound ,030104 developmental biology ,Immune system ,medicine.anatomical_structure ,chemistry ,parasitic diseases ,medicine ,TLR4 ,lipids (amino acids, peptides, and proteins) ,Viability assay ,medicine.symptom - Abstract
Hydrogen sulfide (H2S) is a novel gasotransmitter and acts as a multifunctional regulator in various cellular functions. Past studies have demonstrated a significant role of H2S and its generating enzyme cystathionine gamma-lyase (CSE) in the cardiovascular system. Lipopolysaccharide (LPS), a major pathogenic factor, is known to initiate the inflammatory immune response. The cross talk between LPS-induced inflammation and the CSE/H2S system in vascular cells has not yet been elucidated in detail. Here we showed that LPS decreased CSE mRNA and protein expression in human endothelial cells and blocked H2S production in mouse aorta tissues. Transfection of the cells with TLR4-specific siRNA knockdown TLR4 mRNA expression and abolished the inhibitory role of LPS on CSE expression. Higher dose of LPS (100μg/ml) decreased cell viability, which was reversed by exogenously applied H2S at physiologically relevant concentration (30μM). Lower dose of LPS (10μg/ml) had no effect on cell viability, but significantly induced inflammation gene expressions and cytokines secretion and stimulated cell hyper-permeability. H2S treatment prevented LPS-induced inflammation and hyper-permeability. Lower VE-cadherin expression in LPS-incubated cells would contribute to cell hyper-permeability, which was reversed by H2S co-incubation. In addition, H2S treatment blocked LPS-induced NFκB transactivation. We further validated that LPS-induced hyper-permeability was reversed by CSE overexpression but further deteriorated by CRISPR/Cas9-mediated knockout of CSE. In vivo, deficiency of CSE sensitized the mice to LPS-induced inflammation in vascular tissues. Take together, these data suggest that CSE/H2S system protects LPS-induced inflammation and cell hyper-permeability by blocking NFκB transactivation.
- Published
- 2018
48. Dynamic response and performance of submarine tunnel subjected to surface explosions
- Author
-
Wenbo Lu, Ke Deng, Gaohui Wang, Yong Fan, JinShuai Zhao, and Guangdong Yang
- Subjects
Surface (mathematics) ,Structural safety ,Explosive material ,business.industry ,Mechanical Engineering ,Numerical analysis ,Detonation ,Submarine ,Ocean Engineering ,Structural engineering ,Water depth ,Mechanics of Materials ,General Materials Science ,business ,Geology - Abstract
Recently, external terrorist activities have become one of the most influential events on structural safety because of the absence of proper mechanisms to detect these events. In this study, the effects of surface explosions on the dynamic response and blast resistance of a submarine tunnel are investigated by using a coupled Lagrange and Euler (CLE) method. The feasibility and accuracy of the numerical method and material models are verified against the experimental data. After that, the numerical model is utilized to investigate the dynamic behavior and damage evolution of the submarine tunnel subjected to surface explosions. The dynamic behavior of the tunnel under various detonation scenarios in terms of the explosive weight and water depth is explored. Both the localized damage mechanism and the global structural response of the tunnel are examined. Empirical formulas are proposed to predict the failure modes of tunnel. Besides, studies of tunnel protection against potential attacks by using carbon fibre reinforced polymer (CFRP) and ultra-high performance concrete (UHPC) are also discussed. Numerical results in this study provide tunnel owners and engineers with thorough and important information on the structural performance of submarine tunnels subjected to blast loads, helping them in choosing effective protection strategies for potential explosion events.
- Published
- 2021
49. A SPH-Lagrangian-Eulerian Approach for the Simulation of Concrete Gravity Dams under Combined Effects of Penetration and Explosion
- Author
-
Wenbo Lu, Gaohui Wang, Guangdong Yang, Ming Chen, Xin-xia Wu, and Peng Yan
- Subjects
Shock wave ,Engineering ,Explosive material ,Projectile ,business.industry ,020101 civil engineering ,02 engineering and technology ,Penetration (firestop) ,Penetration test ,0201 civil engineering ,Cracking ,020303 mechanical engineering & transports ,0203 mechanical engineering ,Gravity dam ,Geotechnical engineering ,business ,Underwater explosion ,Civil and Structural Engineering - Abstract
The damage prediction of high dams under the attacks of earth-penetrating weapons has gained significant importance in recent years. For this purpose, a SPH-Lagrangian-Eulerian coupled approach is proposed to describe the damage processes of concrete gravity dams subjected to the combined action of the penetration and explosion. The SPH method is used to model the concrete material with the large deformation near the penetration and explosion regions. The Lagrangian algorithm is adopted to simulate the high-velocity projectile and dam body with the small distortion. And the Eulerian algorithm is employed to describe the dynamic behavior of the water and air media. The validity of the penetration model is calibrated against a previous penetration test. Meanwhile, the SPH-Lagrangian-Eulerian coupled method is verified by implementing an underwater explosion test in a concrete cube. The computed distribution of cracking damage is consistent with the result of the experimental test, which validates the validity of the proposed SPH-Lagrangian-Eulerian coupling method. Subsequently, the penetration processes of a concrete gravity dam under the high-velocity projectile are presented. After the rapid penetration, the explosives are detonated in the dam with the initial penetration damage. The shock wave propagation characteristics in the dam and reservoir water are discussed. The failure processes and dynamic responses of the dam subjected to the combined action of the penetration and explosion are investigated. The influence of the initial penetration damage and the reservoir water on the failure processes of the dam subjected to the internal blast loading is also discussed. The results show that the penetration of the high-velocity projectile only causes a local damage to the concrete gravity dam. However, the combined effects of the penetration and explosion cause significantly more damage to the upper region of the dam.
- Published
- 2017
50. Hydrogen sulfide alleviates the cold stress through MPK4 in Arabidopsis thaliana
- Author
-
Zhuping Jin, Guangdong Yang, Yanxi Pei, Xinzhe Du, and Danmei Liu
- Subjects
0106 biological sciences ,0301 basic medicine ,MAPK/ERK pathway ,Physiology ,Arabidopsis ,Plant Science ,01 natural sciences ,Gene Expression Regulation, Enzymologic ,03 medical and health sciences ,Downregulation and upregulation ,Gene Expression Regulation, Plant ,Genetics ,Arabidopsis thaliana ,Inducer ,Hydrogen Sulfide ,Gene ,biology ,Arabidopsis Proteins ,Cold-Shock Response ,equipment and supplies ,biology.organism_classification ,Cold shock response ,Cell biology ,030104 developmental biology ,Biochemistry ,Mitogen-activated protein kinase ,Plant Stomata ,biology.protein ,Mitogen-Activated Protein Kinases ,010606 plant biology & botany - Abstract
Hydrogen sulfide (H2S) is a gaseous signaling molecule that mediates physiological processes in animals and plants. In this study, we investigated the relationship of H2S and mitogen activated protein kinase (MAPK) under cold stress in Arabidopsis. H2S up-regulated MAPK expression levels and was involved in the cold stress-related upregulation of MAPK genes expression. We then chose MPK4 whose expression level was influenced the most by H2S as a target and found that H2S's ability to alleviate cold stress required MPK4. Both H2S and MPK4 regulated the expression levels of the cold response genes inducer of CBF expression 1 (ICE1), C-repeat-binding factors (CBF3), cold responsive 15A (COR15A) and cold responsive 15B (COR15B). H2S inhibited the opening of stomata under cold stress, which required the participation of MPK4. In conclusion, MPK4 is a downstream component of H2S-related cold-stress resistance, and H2S and MPK4 both regulated the cold response genes and stomatal movement to response the cold stress.
- Published
- 2017
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