92 results on '"Yang, Wu"'
Search Results
2. Targeted metabolomics reveals the association between central carbon metabolism and pulmonary nodules.
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Yue-Yang Wu, Wen-Bin Shen, Jian-Wei Li, Meng-Yu Liu, Wen-Lei Hu, Sheng Wang, Jian-Jun Liu, Fen Huang, and Qi-Rong Qin
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Medicine ,Science - Abstract
With the widespread application of low-dose computed tomography (LDCT) technology, pulmonary nodules have aroused more attention. Significant alteration in plasma metabolite levels, mainly amino acid and lipid, have been observed in patients of PNs. However, evidence on the association between central carbon metabolism and PNs are largely unknown. The aim of this study was to investigate the underlying association of PNs and plasma central carbon metabolites. We measured the levels of 16 plasma central carbon metabolites in 1954 participants who gained LDCT screening in MALSC cohort. The inverse probability weighting (IPW) technique was used to control for bias due to self-selection for LDCT in the assessed high-risk population. The least absolute shrinkage and selection operator (LASSO) penalized regression was used to deal with the problem of multicollinearity among metabolites and the combined association of central carbon metabolites with PNs was estimated by using quantile g-computation (QgC) models. A quartile increase in 3-hydroxybutyric acid, gluconic acid, succinic acid and hippuric acid was positively associated with the PNs risk, whereas a quartile increase in 2-oxadipic acid and fumaric acid was negatively associated with the risk of PNs in multiple-metabolite models. A positive but insignificant joint associations of the mixture of 16 metabolites with PNs was observed by using QgC models analyses. Further studies are warranted to clarify the association between circulating metabolites and PNs and the biological mechanisms.
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- 2023
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3. Carbon peaking prediction scenarios based on different neural network models: A case study of Guizhou Province.
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Lian, Da, Yang, Shi Qiang, Yang, Wu, Zhang, Min, and Ran, Wen Rui
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ARTIFICIAL neural networks ,GREENHOUSE gases ,METAHEURISTIC algorithms ,SUSTAINABLE development ,CARBON emissions - Abstract
Global warming, caused by greenhouse gas emissions, is a major challenge for all human societies. To ensure that ambitious carbon neutrality and sustainable economic development goals are met, regional human activities and their impacts on carbon emissions must be studied. Guizhou Province is a typical karst area in China that predominantly uses fossil fuels. In this study, a backpropagation (BP) neural network and extreme learning machine (ELM) model, which is advantageous due to its nonlinear processing, were used to predict carbon emissions from 2020 to 2040 in Guizhou Province. The carbon emissions were calculated using conversion and inventory compilation methods with energy consumption data and the results showed an "S" growth trend. Twelve influencing factors were selected, however, five with larger correlations were screened out using a grey correlation analysis method. A prediction model for carbon emissions from Guizhou Province was established. The prediction performance of a whale optimization algorithm (WOA)-ELM model was found to be higher than the BP neural network and ELM models. Baseline, high-speed, and low-carbon scenarios were analyzed and the size and time of peak carbon emissions in Liaoning Province from 2020 to 2040 were predicted using the WOA-ELM model. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Time-course transcriptome and WGCNA analysis revealed the drought response mechanism of two sunflower inbred lines.
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Yang Wu, Yaru Wang, Huimin Shi, Haibo Hu, Liuxi Yi, and Jianhua Hou
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Medicine ,Science - Abstract
Drought is one of the most serious abiotic stress factors limiting crop yields. Although sunflower is considered a moderate drought-tolerant plant, drought stress still has a negative impact on sunflower yield as cultivation expands into arid regions. The extent of drought stress is varieties and time-dependent, however, the molecular response mechanisms of drought tolerance in sunflower with different varieties are still unclear. Here, we performed comparative physiological and transcriptome analyses on two sunflower inbred lines with different drought tolerance at the seedling stage. The analysis of nine physiological and biochemical indicators showed that the leaf surface area, leaf relative water content, and cell membrane integrity of drought tolerance inbred line were higher than those of drought-sensitive inbred line under drought stress, indicating that DT had stronger drought resistance. Transcriptome analyses identified 24,234 differentially expressed genes (DEGs). Gene ontology (GO) analysis showed the up-regulated genes were mainly enriched in gibberellin metabolism and rRNA processing, while the down-regulated genes were mainly enriched in cell-wall, photosynthesis, and terpene metabolism. Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway analysis showed genes related to GABAergic synapse, ribosome biogenesis were up-regulated, while genes related with amino sugar and nucleotide sugar metabolism, starch and sucrose metabolism, photosynthesis were down-regulated. Mapman analysis revealed differences in plant hormone-signaling genes over time and between samples. A total of 1,311 unique putative transcription factors (TFs) were identified from all DEGs by iTAK, among which the high abundance of transcription factor families include bHLH, AP2/ERF, MYB, C2H2, etc. Weighted gene co-expression network analysis (WGCNA) revealed a total of 2,251 genes belonging to two modules(blue 4, lightslateblue), respectively, which were significantly associated with six traits. GO and KEGG enrichment analysis of these genes was performed, followed by visualization with Cytoscape software, and the top 20 Hub genes were screened using the CytoHubba plugin.
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- 2022
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5. Acupuncture for glucose and lipid metabolic disorders of polycystic ovarian syndrome: A systematic review protocol
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Yang Wu, Tao Peng, Yu Chen, Li Huang, Bisong He, and Shaobin Wei
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Medicine ,Science - Abstract
Background Polycystic ovary syndrome (PCOS) is a common gynecological disease that is often accompanied by some metabolic abnormality such as insulin resistance and dyslipidemia. As a non-pharmacological therapy, acupuncture is widely used for the treatment of PCOS, but the effectiveness for insulin resistance and lipid metabolic disorder remains controversial. Objectives To assess the effectiveness and safety of acupuncture for insulin resistance and lipid metabolic disorder of women with PCOS. Search methods Eight databases will be searched from inception to June 2021, three clinical trial registration platforms will be searched for relevant trials. Selection criteria Randomized controlled trials (RCTs) of acupuncture therapy for insulin resistance and lipid metabolic of PCOS will be included. Data collection and analysis Study screening, data collection, and analysis will be performed by two or more reviewers independently. We will calculate mean difference (MD), standard mean difference (SMD) with 95% confidence intervals (CIs). Data synthesis will be performed with RevMan V.5.3 software and with Stata V.15.0 software when necessary. PROSPERO registration number CRD42020177846
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- 2021
6. Kinematics and workspace analysis of 4SPRR-SPR parallel robots.
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Lan Luo, Li Hou, Qi Zhang, Yongqiao Wei, and Yang Wu
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Medicine ,Science - Abstract
The 4SPRR-SPR parallel robot, which has considerable potential for application in the field of machining, is a novel closed-loop mechanism with a high rigid-weight ratio. Kinematics and workspace analyses of the 4SPRR-SPR parallel robot are key requirements for its application in machining. In this study, the inverse kinematics of the 4SPRR-SPR parallel robot is analyzed using a geometric method based on the mechanism arrangement of the robot. The forward kinematics model is derived by training the vector-quantified temporal associative memory (VQTAM) network, which originates from a self-organizing map (SOM). Furthermore, an improved algorithm is obtained by combining the locally linear embedding (LLE) and VQTAM methods. A boundary extraction algorithm for the workspace analysis of the parallel robot is proposed. The performance of the boundary extraction algorithm is analyzed and compared with that of a global search algorithm; the result indicates that the novel algorithm has the same computational accuracy in addition to higher efficiency. The workspace of the 4SPRR-SPR parallel robot is analyzed using the boundary extraction algorithm. Finally, the 3D model of the 4SPRR-SPR parallel robot is simulated using the ADAMS software to verify the reliability of the proposed algorithms. The simulation results demonstrate the effectiveness of the methods proposed in this study. In addition, the robot kinematics and workspace analysis methods described herein can be extended to other serial and parallel robots. This research provides a theoretical framework for trajectory planning of mechanisms, workspace optimization of robots, and robotic control.
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- 2021
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7. Does catheter material affect functional performance of intravenous ports via the superior vena cava?
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Ching-Feng Wu, Jui-Ying Fu, Tsai-Yang Hsieh, Chi-Tsung Wen, Sheng-Yueh Yu, Ming-Ju Hsieh, Yen Chu, and Ching-Yang Wu
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Medicine ,Science - Abstract
Introduction The catheter is the only intravascular portion of an implanted port and plays a crucial role in catheter related complications. Both polyurethane and silicone are biocompatible materials which are utilized for catheter manufacturing, but their correlation to complications remains controversial. The aim of this study was to try to analyze the relationship between catheter materials and complications. Materials and methods A total of 3144 patients who underwent intravenous port implantation between March 2012 and December 2018 at Chang Gung Memorial Hospital, Linkou, Taiwan were recruited. Of these, 1226 patients received silicone catheter port implantation and 1679 received polyurethane catheter ports. Case matching was done prior to analysis and catheter related complications and cumulative complication incidence for each group were compared. Results Intergroup differences were identified in entry vessel (p = 0.0441), operation year (p < 0.0001), operation method (p = 0.0095), functional period (p < 0.0001), patient follow up status (p < 0.0001), operating time for vessel cutdown (p < 0.0001) and wire assisted approach (p = 0.0008). Stratified by specific entry vessel, no statistical difference was found in complication rate or incidence between the silicone and polyurethane groups. We further compared the cumulative complication incidence of the silicone and polyurethane groups, and also found no statistical difference (p = 0.4451). Conclusion As long as external stress forces generated by surrounding structures and focused on potential weak points are avoided, both silicone and polyurethane materials provide sufficient structural stability to serve as reliable vascular access for patients.
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- 2021
8. Evaluation of single nucleotide polymorphisms in 6 candidate genes and carotid intima-media thickness in community-dwelling residents.
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Fang-Yang Wu, Chia-Ing Li, Li-Na Liao, Chiu-Shong Liu, Wen-Yuan Lin, Chih-Hsueh Lin, Chuan-Wei Yang, Tsai-Chung Li, and Cheng-Chieh Lin
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Medicine ,Science - Abstract
Evidence suggests the existence of association between a large panel of modifiable biomarkers representing inflammation, coagulation, paraoxonase, and endothelial activation pathways and carotid atherosclerosis. Thus, this study investigated whether CRP, FGA, FGB, FGG, PON1, and EDNRA gene variants affected plasma hs-CRP, fibrinogen levels, and thickness of carotid intima media thickness (IMT). Nineteen single-nucleotide polymorphisms of CRP, FGA, FGB, FGG, PON1, and EDNRA genes were examined in 480 participants from 160 families. Carotid IMT was measured by ultrasound. Generalized linear models with generalized estimating equation were utilized to consider the dependence of subjects within families. In the recessive model, homozygotes for the minor alleles of rs1800789, rs1800790 and rs4220 SNPs in FGB gene indicated a reduced risk of IMT (Exp. β = 0.89, 0.89, 0.88), which remained significant after adjustment for confounding factors. Significant interaction effects between CRP SNP rs1130864 and rs3093059 and gender for IMT were observed with a significant association in men only. Men carrying minor-minor genotype of CRP SNP rs1130864 and rs3093059 had 0.70- and 0.78-fold lower IMT than men carrying minor-major/major-major genotype. We also observed that the interaction of CRP SNP rs1130864 and rs3093059 with obesity on IMT, hs-CRP and fibrinogen levels. These results support the hypothesis that inflammatory genes are involved in atherosclerosis, most likely via complex gene-gender and gene-obesity interactions.
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- 2020
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9. Interactions among IGF-1, AKT2, FOXO1, and FOXO3 variations and between genes and physical activities on physical performance in community-dwelling elders.
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Tsai-Chung Li, Ching-Wei Wu, Chia-Ing Li, Fang-Yang Wu, Li-Na Liao, Chiu-Shong Liu, Chih-Hsueh Lin, Mu-Cyun Wang, Chuan-Wei Yang, and Cheng-Chieh Lin
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Medicine ,Science - Abstract
This study assessed the interactions among IGF-1, AKT2, FOXO1, and FOXO3 variations and the interactions of gene and physical activity on handgrip strength, arm muscle mass-adjusted handgrip (armGrip), gait speed (GS), timed up and go (TUG), and leg press strength (LPS). Nine single nucleotide polymorphisms (SNPs) containing three IGF-1 SNPs (rs6214, rs5742692, and rs35767), two AKT2 SNPs (rs892119 and rs35817154), two FOXO1 SNPs (rs17446593 and rs10507486), and two FOXO3 SNPs (rs9480865 and rs2153960) were genotyped in 472 unrelated elders with a mean age of 73.8 years. We observed significant interactions of IGF-1 SNP rs6214 and rs35767 with regular physical activity on TUG and GS; and AKT2 SNP rs892119 and FOXO3 SNP rs9480865 with regular physical activity on armGrip. Genotype GG of IGF-1 rs6214 and rs35767 in individuals without regular physical activity had poor performance in TUG and GS, as well as GG of AKT2 rs892119 decreased armGrip in individuals without regular physical activity. After FDR adjustment, no significant gene-gene interactions were found. A sedentary lifestyle may increase the risk of impairing physical performance and regular physical activity is a remedy for sarcopenia, even a little regular physical activity can overcome carrying some risk alleles in this pathway.
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- 2020
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10. Important gene-gene interaction of TNF-α and VDR on osteoporosis in community-dwelling elders.
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Li-Na Liao, Chia-Ing Li, Fang-Yang Wu, Chuan-Wei Yang, Chih-Hsueh Lin, Chiu-Shong Liu, Wen-Yuan Lin, Tsai-Chung Li, and Cheng-Chieh Lin
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Medicine ,Science - Abstract
Gene effects on osteoporosis have been studied separately and may have been masked by gene-gene and gene-environment interactions. We evaluated gene-gene and gene-physical activity interactions of the variants of tumor necrosis factor-α (TNF-α) and vitamin D receptor (VDR) genes on osteoporosis. A total of 472 elders were included. Seven variants (TNF-α: rs1799964, rs1800629, rs3093662; VDR: rs7975232, rs1544410, rs2239185, rs3782905) were genotyped. Bone mineral densities of the lumbar spine, femoral neck, and total hip were measured by dual-energy X-ray absorptiometry. Predictive models' ability to discriminate osteoporosis status was evaluated by areas under the receiver operating characteristics (AUROC) curve. After multivariable adjustment, significant interactions of TNF-α rs1800629 and VDR rs3782905 were observed on overall and lumbar spine osteoporosis. In elderly women, we found that those carrying the CG/CC genotype of VDR rs3782905 were significantly associated with increased odds of overall osteoporosis compared with those carrying the GG genotype of VDR rs3782905 among those carrying TNF-α rs1800629 GG genotype. The adjusted odds ratios (ORs) for VDR rs3782905 CG/CC genotype in elderly women carrying TNF-α rs1800629 AG/AA and GG genotypes were 0.1 (0.01, 0.98) and 3.54 (1.51, 8.30), respectively. We observed significant differences in AUROCs between the model with traditional covariates plus variants and their interaction term and the model with traditional covariates only (AUROCs: 0.77 and 0.81; p = 0.028). Although the sample size of this study may have been relatively small, our results suggest that the interaction of the CG/CC genotype of VDR rs3782905 with TNF-α rs1800629 GG genotype was associated with increased odds of overall and lumbar spine osteoporosis in elderly women.
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- 2019
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11. Correction: Correlation between image characteristics and pathologic findings in non small cell lung cancer patients after anatomic resection.
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Jui-Ying Fu, Yung-Liang Wan, Tzu-Yen Huang, Ching-Feng Wu, Yun-Hen Liu, Ming-Ju Hsieh, Yi-Cheng Wu, and Ching-Yang Wu
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Medicine ,Science - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0206386.].
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- 2019
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12. Recommended irrigation volume for an intravenous port: Ex vivo simulation study.
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Ching-Yang Wu, Chia-Hui Cheng, Jui-Ying Fu, Yen Chu, Ching-Feng Wu, Chien-Hung Chiu, Po-Jen Ko, and Yun-Hen Liu
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Medicine ,Science - Abstract
BACKGROUND:An intravenous port, which differs from a central venous catheter, has an injection chamber at the end of the catheter. This structural difference causes the irrigation flow pattern to be quite different from that of the central venous catheter. Furthermore, the intraluminal volume differs due to the size of the injection chamber and implanted catheter length. Hence, the ideal recommended irrigation volume varies because of differences in intraluminal volume, however, the recommended irrigation volume is 10 ml and may be a cause for reported port malfunctions. This study investigates the best irrigation volume for an intravenous port by simulating the clinical scenario ex-vivo to access its usefulness. MATERIALS AND METHODS:This study was composed of two tests. The irrigation volume test attempted to quantify the irrigation volume of an implanted port while the irrigation rate test attempted to simulate daily nursing practice in order to clarify the effect of irrigation flow. The human blood needed for the simulation was donated by volunteers and the total volume was 10 ml per test. The irrigation volume test was done by syringe pump with varying pre-set irrigation volume after the port and connected catheter were filled with volunteer blood. After irrigation with pre-set volume, the retained intraluminal solution was collected and quantified by Bradford assay in order to titrate the best irrigation volume. The irrigation rate test tried to simulate daily maintenance practice in different settings with the quantified irrigation volume as identified by the irrigation volume test. The retained intraluminal solution was collected and quantified by Bradford assay in order to confirm the efficacy of the quantified irrigation volume. RESULTS:In both SVC and IVC ports, we identified the twenty times the intravascular volume as sufficient for a complete wash out of the blood component in the irrigation volume test. The minimal irrigation volume for SVC and IVC port were 10 ml and 15.6 ml respectively. In irrigation rate test, the irrigation for SVC and IVC port was 10 and 20 ml, respectively, for the sake of preparation convenience. We not only identified the importance of preparation, i.e. irrigation of the extension line but also confirmed the efficacy of the recommended irrigation volume. CONCLUSION:The irrigation volume should be varied according to the intraluminal volume. Maintenance should be performed after the extension line has been irrigated. The recommended port irrigation volume for SVC and IVC route were 10 and 20 ml, respectively.
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- 2018
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13. Correlation between image characteristics and pathologic findings in non small cell lung cancer patients after anatomic resection.
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Jui-Ying Fu, Yung-Liang Wan, Tzu-Yen Huang, Ching-Feng Wu, Yun-Hen Liu, Ming-Ju Hsieh, Yi-Cheng Wu, and Ching-Yang Wu
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Medicine ,Science - Abstract
INTRODUCTION:Tumor characteristics in computed tomography (CT) are correlated to pathologic presentation and survival. However, most studies have been based on thin slice thickness CT while lymph node metastatic pattern has remained unclear. The aim of this study was to analyze the correlation between image characteristics under 5 mm slice thickness and pathology findings in non small lung cancer patients who have received curative resection. MATERIALS AND METHODS:From January 2010 to May 2014, 440 patients who underwent curative resection were included and medical records were reviewed retrospectively. The tumor size and consolidation tumor ratio were simultaneously evaluated and measured by a physician, a thoracic surgeon, and a radiologist. The correlation between image and pathology characteristics and its survival impact was analyzed. RESULTS:Tumor sizes, as measured by CT and by pathologic measurement were highly coincident. (p < 0.001) GGO predominant lesions were correlated to well-differentiated adenocarcinoma, (p< 0.001), and less tumor necrosis (p
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- 2018
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14. 5-aminolevulinic acid-mediated photodynamic therapy and its strain-dependent combined effect with antibiotics on Staphylococcus aureus biofilm.
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Qing-Zhao Zhang, Ke-Qing Zhao, Yang Wu, Xian-Hui Li, Chen Yang, Li-Min Guo, Chun-Hong Liu, Di Qu, and Chun-Quan Zheng
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Medicine ,Science - Abstract
Staphylococcus aureus (S. aureus) is hard to be eradicated, not only due to the emergence of antibiotic resistant strains but also because of its ability to form biofilm. Antibiotics are the major approach to treating biofilm infections, but their effects are unsatisfactory. One of the potential alternative treatments for controlling biofilm infections is photodynamic therapy (PDT), which requires the administration of photosensitizer, followed by light activation. 5-aminolevulinic acid (ALA), a natural photosensitizer prodrug, presents favorable characteristics, such as easy penetration and rapid clearance. These advantages enable ALA-based PDT (ALA-PDT) to be well-tolerated by patients and it can be repeatedly applied without cumulative toxicity or serious side effects. ALA-PDT has been proven to be an effective treatment for multidrug resistant pathogens; however, the study of its effect on S. aureus biofilm is limited. Here, we established our PDT system based on the utilization of ALA and a light-emitting diode, and we tested the effect of ALA-PDT on S. aureus biofilm as well as the combined effect of ALA-PDT and antibiotics on S. aureus biofilm. Our results showed that ALA-PDT has a strong antibacterial effect on S. aureus biofilm, which was confirmed by the confocal laser scanning microscope. We also found that lethal photosensitization occurred predominantly in the upper layer of the biofilm, while the residual live bacteria were located in the lower layer of the biofilm. In addition, the improved bactericidal effect was observed in the combined treatment group but in a strain-dependent manner. Our results suggest that ALA-PDT is a potential alternative approach for future clinical use to treat S. aureus biofilm-associated infections, and some patients may benefit from the combined treatment of ALA-PDT and antibiotics, but drug sensitivity testing should be performed in advance.
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- 2017
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15. Effect of continuous positive airway pressure on carotid intima-media thickness in patients with obstructive sleep apnea: A meta-analysis.
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Li-Da Chen, Li Lin, Xue-Jun Lin, Yang-Wu Ou, Zhi Wu, Yu-Ming Ye, Qiao-Zhen Xu, Ya-Ping Huang, and Zhi-Ming Cai
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Medicine ,Science - Abstract
Obstructive sleep apnea (OSA) is associated with increased carotid intima-media thickness (IMT), an early marker of atherosclerosis. Continuous positive airway pressure (CPAP) is the first-line treatment for OSA. A meta-analysis was performed to determine whether CPAP therapy could decrease carotid IMT.The PubMed, Embase, Web of Science, and Cochrane library were searched before March, 2017. Weighted mean difference (WMD) was calculated to estimate the treatment effects of pre and post-CPAP therapy. Seven studies were examined and the meta-analysis was performed using STATA 12.0.There was no change of carotid IMT before and after CPAP treatment in OSA patients (WMD = 0.052, 95% confidence interval (CI) = -0.002 to 0.105, z = 1.90, p = 0.057). Meanwhile, meta-analysis of the two RCTs showed that carotid IMT was not changed in CPAP group when compared with control group (WMD = 0.002 95% CI = -0.125 to 0.129, z = 0.03, p = 0.976). Subgroup analyses indicated that carotid IMT was significantly decreased after CPAP use in more severe OSA patients (AHI≥50) (WMD = 0.073, 95% CI = 0.022 to 0.124, z = 2.80, p = 0.005) and patients with therapeutic duration ≥6 months (WMD = 0.121, 95% CI = 0.019 to 0.223, z = 2.32, p = 0.021).CPAP had no impact on carotid IMT in OSA patients. However, carotid IMT was significantly decreased after CPAP treatment in more severe OSA patients and patients with longer CPAP usage.
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- 2017
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16. Prognostic implication of EGFR gene mutations and histological classification in patients with resected stage I lung adenocarcinoma.
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Chun-Yu Lin, Yen-Mu Wu, Meng-Heng Hsieh, Chih-Wei Wang, Ching-Yang Wu, Ying-Jen Chen, and Yueh-Fu Fang
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Medicine ,Science - Abstract
The prognostic value of epidermal growth factor receptor (EGFR) mutations and the correlation between EGFR mutations and the new International Association for the Study of Lung Cancer/American Thoracic Society/European Respiratory Society (IASLC/ATS/ERS) histological classification remain controversial. The current study aimed to investigate the pure prognostic role of EGFR mutations in treatment-naïve patients with resected stage I lung adenocarcinoma.We retrospectively reviewed 373 patients with stage I pulmonary non-small-cell lung cancer who underwent complete surgical resection between January 2010 and May 2014. The tumors were classified according to IASLC/ATS/ERS criteria. EGFR mutation status was determined by established methods.A total of 120 patients were included for analysis; 87 had tumors with EGFR mutations and 33 had wild-type tumors. More low- and intermediate-grade tumors had EGFR mutations, and nearly half of the high-grade tumors were wild-type (75.7% versus 46.2%, p = 0.041). Patients with low-grade tumors had significantly greater median disease-free survival (DFS) (76.8 versus 13 months, p < 0.0001) and better overall survival (OS) (median OS not reached, p = 0.0003) than those with intermediate- and high-grade tumors. Tumor recurrence was 41.4% and 30.3% in mutant and wild-type patients. The 5-years survival rate was 54% and 71.2%. Multivariate analysis revealed that the new histological classification and the pathologic stage were independent predictors of both DFS and OS. EGFR mutation status had no prognostic implications.Low grade tumors according to IASLC/ATS/ERS histological classification and the pathologic stage IA tumors of resected stage I lung adenocarcinomas independently predict better DFS and OS. EGFR mutations were frequently seen in histologically low- and intermediate-grade tumors but not a prognostic factor.
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- 2017
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17. Identification of a Group of GABAergic Neurons in the Dorsomedial Area of the Locus Coeruleus.
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Xin Jin, Shanshan Li, Brian Bondy, Weiwei Zhong, Max F Oginsky, Yang Wu, Christopher M Johnson, Shuang Zhang, Ningren Cui, and Chun Jiang
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Medicine ,Science - Abstract
The locus coeruleus (LC)-norepinephrine (NE) system in the brainstem plays a critical role in a variety of behaviors is an important target of pharmacological intervention to several neurological disorders. Although GABA is the major inhibitory neurotransmitter of LC neurons, the modulation of LC neuronal firing activity by local GABAergic interneurons remains poorly understood with respect to their precise location, intrinsic membrane properties and synaptic modulation. Here, we took an optogenetic approach to address these questions. Channelrhodopsin (ChR2) in a tandem with the yellow fluorescent protein (YFP) was expressed in GABAergic neurons under the control of glutamic acid decarboxylase 2 (GAD2) promoter. Immediately dorsomedial to the LC nucleus, a group of GABAergic neurons was observed. They had small soma and were densely packed in a small area, which we named the dorsomedial LC or dmLC nucleus. These GABAergic neurons showed fast firing activity, strong inward rectification and spike frequency adaptation. Lateral inhibition among these GABAergic neurons was observed. Optostimulation of the dmLC area drastically inhibited LC neuronal firing frequency, expanded the spike intervals, and reset their pacemaking activity. Analysis of the light evoked inhibitory postsynaptic currents (IPSCs) indicated that they were monosynaptic. Such light evoked IPSCs were not seen in slices where this group of GABAergic neurons was absent. Thus, an isolated group of GABAergic neurons is demonstrated in the LC area, whose location, somatic morphology and intrinsic membrane properties are clearly distinguishable from adjacent LC neurons. They interact with each and may inhibit LC neurons as well as a part of local neuronal circuitry in the LC.
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- 2016
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18. Time Trends in Fast Food Consumption and Its Association with Obesity among Children in China.
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Hong Xue, Yang Wu, Xiaoyu Wang, and Youfa Wang
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Medicine ,Science - Abstract
OBJECTIVE:Study the trends in Western fast food consumption (FFC) among Chinese school-age children and the association between FFC and obesity using nationwide survey data. DESIGN:Cross-sectional and longitudinal analyses were conducted to study the trends in FFC and the associations between FFC and weight status (overweight, obesity and body mass index (BMI) z-score). SETTING:Longitudinal data from families were collected in the 2004 and 2009 China Health and Nutrition Survey (covering nine provinces throughout China). SUBJECTS:The analysis included 2656 Chinese children aged 6 to 18 years (1542 and 1114 children in the 2004 and 2009 survey, respectively). RESULTS:FFC (reported having consumed Western fast food in the past three months) has increased between 2004 and 2009, from 18.5% to 23.9% in those aged 6-18, and increased more rapidly among those aged 13-17, from 17.9% to 26.3%. The increase was significant in almost all groups by age, sex, family income, and residence. Our cross-sectional and longitudinal analyses did not detect a significant association between FFC and obesity/overweight or BMI z-score (e.g., for BMI z-score, boys: β = 0.02, 95% CI: -0.71, 0.75; girls: β = -0.14, 95% CI: -1.03, 0.75). CONCLUSIONS:FFC has increased in Chinese school-age children, especially in older children, boys, and those from low- and medium-income families, rural areas, and East China, but decreased among those from high-income families during 2004-2009. The data did not show a significant association between FFC and obesity.
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- 2016
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19. The Impact of Variable Degrees of Freedom and Scale Parameters in Bayesian Methods for Genomic Prediction in Chinese Simmental Beef Cattle.
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Bo Zhu, Miao Zhu, Jicai Jiang, Hong Niu, Yanhui Wang, Yang Wu, Lingyang Xu, Yan Chen, Lupei Zhang, Xue Gao, Huijiang Gao, Jianfeng Liu, and Junya Li
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Medicine ,Science - Abstract
Three conventional Bayesian approaches (BayesA, BayesB and BayesCπ) have been demonstrated to be powerful in predicting genomic merit for complex traits in livestock. A priori, these Bayesian models assume that the non-zero SNP effects (marginally) follow a t-distribution depending on two fixed hyperparameters, degrees of freedom and scale parameters. In this study, we performed genomic prediction in Chinese Simmental beef cattle and treated degrees of freedom and scale parameters as unknown with inappropriate priors. Furthermore, we compared the modified methods (BayesFA, BayesFB and BayesFCπ) with their corresponding counterparts using simulation datasets. We found that the modified methods with distribution assumed to the two hyperparameters were beneficial for improving the predictive accuracy. Our results showed that the predictive accuracies of the modified methods were slightly higher than those of their counterparts especially for traits with low heritability and a small number of QTLs. Moreover, cross-validation analysis for three traits, namely carcass weight, live weight and tenderloin weight, in 1136 Simmental beef cattle suggested that predictive accuracy of BayesFCπ noticeably outperformed BayesCπ with the highest increase (3.8%) for live weight using the cohort masking cross-validation.
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- 2016
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20. Correction: An Analysis of the Binding Characteristics of a Panel of Recently Selected ICAM-1 Binding Plasmodium falciparum Patient Isolates.
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Aymen M Madkhali, Mohammed O Alkurbi, Tadge Szestak, Anja Bengtsson, Pradeep R Patil, Yang Wu, Saeed Alharthi, Anja T R Jensen, Richard Pleass, and Alister G Craig
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Medicine ,Science - Published
- 2016
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21. Outcomes and Treatment of Lumbosacral Spinal Tuberculosis: A Retrospective Study of 53 Patients.
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Tenglong Jiang, Jinming Zhao, Maolin He, Kun Wang, Mitra Fowdur, and Yang Wu
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Medicine ,Science - Abstract
A retrospective clinic study.To evaluate the efficacy of conservative and surgical treatment for lumbosacral tuberculosis.This study retrospectively reviewed 53 patients with lumbosacral tuberculosis who were treated in our institution between January 2005 and January 2011. There were 29 males and 24 females with average ages of 37.53 ± 17.28 years (range 6-72 years). 11 patients were given only anti-TB drugs; the remainder underwent anterior debridement, interbody fusion with and without instrumentation, or one-stage anterior debridement combined with posterior instrumentation. Outcome data for these patients included neurologic status, lumbosacral angle, erythrocyte sedimentation rate value(ESR) and C-reactive protein value(CRP) were assessed before and after treatment.The mean lumbosacral angles were 23.00°± 2.90° in the conservatively treated patients and 22.36°± 3.92o in the surgically treated patients. At the final follow-up, this had improved to 24.10o ± 2.96° in the conservatively treated patients and 28.13° ± 1.93° in the surgically treated patients (all P < 0.05). There were statistically significant differences before and after treatment in terms of ESR and CRP (all P < 0.05). All patients achieved bone fusion. The mean follow-up period was 32.34 ± 8.13 months (range 18 to 55 months). The neurological deficit did not worsen in any of the patients.It has been proven that conservative and surgical treatments are safe and effective and produce good clinical outcomes for patients with lumbosacral tuberculosis. The advantages of operation include thoroughness of debridement, decompression of the spinal cord, and adequate spinal stabilization.
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- 2015
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22. Polydatin Restores Endothelium-Dependent Relaxation in Rat Aorta Rings Impaired by High Glucose: A Novel Insight into the PPARβ-NO Signaling Pathway.
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Yang Wu, Lai Xue, Weimin Du, Bo Huang, Cuiping Tang, Changqing Liu, Hongmei Qiu, and Qingsong Jiang
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Medicine ,Science - Abstract
Polydatin, a natural component from Polygonum Cuspidatum, has important therapeutic effects on metabolic syndrome. A novel therapeutic strategy using polydatin to improve vascular function has recently been proposed to treat diabetes-related cardiovascular complications. However, the biological role and molecular basis of polydatin's action on vascular endothelial cells (VECs)-mediated vasodilatation under diabetes-related hyperglycemia condition remain elusive. The present study aimed to assess the contribution of polydatin in restoring endothelium-dependent relaxation and to determine the details of its underlying mechanism. By measuring endothelium-dependent relaxation, we found that acetylcholine-induced vasodilation was impaired by elevated glucose (55 mmol/L); however, polydatin (1, 3, 10 μmol/L) could restore the relaxation in a dose-dependent manner. Polydatin could also improve the histological damage to endothelial cells in the thoracic aorta. Polydatin's effects were mediated via promoting the expression of endothelial NO synthase (eNOS), enhancing eNOS activity and decreasing the inducible NOS (iNOS) level, finally resulting in a beneficial increase in NO release, which probably, at least in part, through activation of the PPARβ signaling pathway. The results provided a novel insight into polydatin action, via PPARβ-NO signaling pathways, in restoring endothelial function in high glucose conditions. The results also indicated the potential utility of polydatin to treat diabetes related cardiovascular diseases.
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- 2015
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23. Acupuncture for glucose and lipid metabolic disorders of polycystic ovarian syndrome: A systematic review protocol
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Bisong He, Shaobin Wei, Li Huang, Yu Chen, Tao Peng, and Yang Wu
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Physiology ,Acupuncture Therapy ,Biochemistry ,law.invention ,Database and Informatics Methods ,Medical Conditions ,Endocrinology ,Randomized controlled trial ,Glucose Metabolism ,law ,Medicine and Health Sciences ,Database Searching ,Randomized Controlled Trials as Topic ,Multidisciplinary ,Metabolic disorder ,Research Assessment ,Polycystic ovary ,Lipids ,Oncology ,Medicine ,Carbohydrate Metabolism ,Female ,Polycystic Ovary Syndrome ,medicine.medical_specialty ,Systematic Reviews ,Science ,MEDLINE ,Research and Analysis Methods ,Insulin resistance ,Complementary and Alternative Medicine ,Internal medicine ,Registered Report Protocol ,medicine ,Acupuncture ,Humans ,Endocrine Physiology ,business.industry ,Biology and Life Sciences ,Cancers and Neoplasms ,medicine.disease ,Lipid Metabolism ,Clinical trial ,Glucose ,Metabolism ,Metabolic Disorders ,Insulin Resistance ,business ,Gynecological Tumors ,Dyslipidemia - Abstract
Background Polycystic ovary syndrome (PCOS) is a common gynecological disease that is often accompanied by some metabolic abnormality such as insulin resistance and dyslipidemia. As a non-pharmacological therapy, acupuncture is widely used for the treatment of PCOS, but the effectiveness for insulin resistance and lipid metabolic disorder remains controversial. Objectives To assess the effectiveness and safety of acupuncture for insulin resistance and lipid metabolic disorder of women with PCOS. Search methods Eight databases will be searched from inception to June 2021, three clinical trial registration platforms will be searched for relevant trials. Selection criteria Randomized controlled trials (RCTs) of acupuncture therapy for insulin resistance and lipid metabolic of PCOS will be included. Data collection and analysis Study screening, data collection, and analysis will be performed by two or more reviewers independently. We will calculate mean difference (MD), standard mean difference (SMD) with 95% confidence intervals (CIs). Data synthesis will be performed with RevMan V.5.3 software and with Stata V.15.0 software when necessary. PROSPERO registration number CRD42020177846
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- 2021
24. Does catheter material affect functional performance of intravenous ports via the superior vena cava?
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Jui-Ying Fu, Sheng-Yueh Yu, Yen Chu, Chi-Tsung Wen, Ming-Ju Hsieh, Ching-Feng Wu, Tsai-Yang Hsieh, and Ching-Yang Wu
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Male ,Medical Implants ,Polymers ,Polyurethanes ,Silicones ,Cannulation ,Jugular Vein ,Vascular Medicine ,chemistry.chemical_compound ,Catheters, Indwelling ,Jugular vein ,Medicine and Health Sciences ,Materials ,Multidisciplinary ,Incidence ,Venous Thromboembolism ,Middle Aged ,Physical Functional Performance ,Deep Vein Thrombosis ,Catheter ,Chemistry ,Macromolecules ,Physical Sciences ,Engineering and Technology ,Medicine ,Administration, Intravenous ,Equipment Failure ,Female ,Anatomy ,Research Article ,Biotechnology ,medicine.medical_specialty ,Catheterization, Central Venous ,Catheters ,Vena Cava, Superior ,Science ,Materials Science ,Biomaterial Implants ,Vascular access ,Taiwan ,Bioengineering ,Surgical and Invasive Medical Procedures ,Veins ,Silicone ,Superior vena cava ,Thromboembolism ,medicine ,Operating time ,Humans ,business.industry ,Chemical Compounds ,Biology and Life Sciences ,Biocompatible material ,Polymer Chemistry ,Surgery ,chemistry ,Cardiovascular Anatomy ,Blood Vessels ,Medical Devices and Equipment ,Complication ,business - Abstract
Introduction The catheter is the only intravascular portion of an implanted port and plays a crucial role in catheter related complications. Both polyurethane and silicone are biocompatible materials which are utilized for catheter manufacturing, but their correlation to complications remains controversial. The aim of this study was to try to analyze the relationship between catheter materials and complications. Materials and methods A total of 3144 patients who underwent intravenous port implantation between March 2012 and December 2018 at Chang Gung Memorial Hospital, Linkou, Taiwan were recruited. Of these, 1226 patients received silicone catheter port implantation and 1679 received polyurethane catheter ports. Case matching was done prior to analysis and catheter related complications and cumulative complication incidence for each group were compared. Results Intergroup differences were identified in entry vessel (p = 0.0441), operation year (p < 0.0001), operation method (p = 0.0095), functional period (p < 0.0001), patient follow up status (p < 0.0001), operating time for vessel cutdown (p < 0.0001) and wire assisted approach (p = 0.0008). Stratified by specific entry vessel, no statistical difference was found in complication rate or incidence between the silicone and polyurethane groups. We further compared the cumulative complication incidence of the silicone and polyurethane groups, and also found no statistical difference (p = 0.4451). Conclusion As long as external stress forces generated by surrounding structures and focused on potential weak points are avoided, both silicone and polyurethane materials provide sufficient structural stability to serve as reliable vascular access for patients.
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- 2021
25. Interactions among IGF-1, AKT2, FOXO1, and FOXO3 variations and between genes and physical activities on physical performance in community-dwelling elders
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Cheng-Chieh Lin, Ching-Wei Wu, Li-Na Liao, Fang-Yang Wu, Chih Hsueh Lin, Chia-Ing Li, Chuan-Wei Yang, Chiu-Shong Liu, Mu-Cyun Wang, and Tsai-Chung Li
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Male ,Sarcopenia ,Heredity ,Single Nucleotide Polymorphisms ,0302 clinical medicine ,Elderly ,Gene Frequency ,Polymorphism (computer science) ,Medicine and Health Sciences ,Public and Occupational Health ,Biomechanics ,030212 general & internal medicine ,Insulin-Like Growth Factor I ,Leg press ,Musculoskeletal System ,Multidisciplinary ,Hand Strength ,Forkhead Box Protein O1 ,Muscles ,Forkhead Box Protein O3 ,Physical Functional Performance ,Genetic Mapping ,Medicine ,Legs ,Female ,Anatomy ,Research Article ,medicine.medical_specialty ,Genotype ,Science ,030209 endocrinology & metabolism ,Single-nucleotide polymorphism ,Variant Genotypes ,Biology ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,Signs and Symptoms ,Hand strength ,Internal medicine ,medicine ,Genetics ,SNP ,Adults ,Humans ,Exercise physiology ,Exercise ,Alleles ,Sedentary lifestyle ,Aged ,Biology and Life Sciences ,Physical Activity ,medicine.disease ,Endocrinology ,Skeletal Muscles ,Age Groups ,Body Limbs ,People and Places ,Population Groupings ,Clinical Medicine ,Sedentary Behavior ,human activities ,Proto-Oncogene Proteins c-akt - Abstract
This study assessed the interactions among IGF-1, AKT2, FOXO1, and FOXO3 variations and the interactions of gene and physical activity on handgrip strength, arm muscle mass-adjusted handgrip (armGrip), gait speed (GS), timed up and go (TUG), and leg press strength (LPS). Nine single nucleotide polymorphisms (SNPs) containing three IGF-1 SNPs (rs6214, rs5742692, and rs35767), two AKT2 SNPs (rs892119 and rs35817154), two FOXO1 SNPs (rs17446593 and rs10507486), and two FOXO3 SNPs (rs9480865 and rs2153960) were genotyped in 472 unrelated elders with a mean age of 73.8 years. We observed significant interactions of IGF-1 SNP rs6214 and rs35767 with regular physical activity on TUG and GS; and AKT2 SNP rs892119 and FOXO3 SNP rs9480865 with regular physical activity on armGrip. Genotype GG of IGF-1 rs6214 and rs35767 in individuals without regular physical activity had poor performance in TUG and GS, as well as GG of AKT2 rs892119 decreased armGrip in individuals without regular physical activity. After FDR adjustment, no significant gene-gene interactions were found. A sedentary lifestyle may increase the risk of impairing physical performance and regular physical activity is a remedy for sarcopenia, even a little regular physical activity can overcome carrying some risk alleles in this pathway.
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- 2020
26. Kinematics and workspace analysis of 4SPRR-SPR parallel robots
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Lan Luo, Yongqiao Wei, Yang Wu, Qi Zhang, and Li Hou
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0209 industrial biotechnology ,Kinematics ,Computer science ,02 engineering and technology ,Machine Learning ,020901 industrial engineering & automation ,Search algorithm ,Animal Cells ,0202 electrical engineering, electronic engineering, information engineering ,Neurons ,Robot kinematics ,Multidisciplinary ,Robotic control ,Physics ,Applied Mathematics ,Simulation and Modeling ,Parallel manipulator ,Classical Mechanics ,Robotics ,Biomechanical Phenomena ,Physical Sciences ,Medicine ,Engineering and Technology ,020201 artificial intelligence & image processing ,Cellular Types ,Algorithm ,Robots ,Algorithms ,Research Article ,Forward kinematics ,Computer and Information Sciences ,Neural Networks ,Science ,Acceleration ,Workspace ,Research and Analysis Methods ,Computer Science::Robotics ,Machine Learning Algorithms ,Artificial Intelligence ,Computer Simulation ,Inverse kinematics ,Mechanical Engineering ,Reproducibility of Results ,Biology and Life Sciences ,Cell Biology ,Surface Plasmon Resonance ,Cellular Neuroscience ,Robot ,Software ,Mathematics ,Actuators ,Neuroscience - Abstract
The 4SPRR-SPR parallel robot, which has considerable potential for application in the field of machining, is a novel closed-loop mechanism with a high rigid-weight ratio. Kinematics and workspace analyses of the 4SPRR-SPR parallel robot are key requirements for its application in machining. In this study, the inverse kinematics of the 4SPRR-SPR parallel robot is analyzed using a geometric method based on the mechanism arrangement of the robot. The forward kinematics model is derived by training the vector-quantified temporal associative memory (VQTAM) network, which originates from a self-organizing map (SOM). Furthermore, an improved algorithm is obtained by combining the locally linear embedding (LLE) and VQTAM methods. A boundary extraction algorithm for the workspace analysis of the parallel robot is proposed. The performance of the boundary extraction algorithm is analyzed and compared with that of a global search algorithm; the result indicates that the novel algorithm has the same computational accuracy in addition to higher efficiency. The workspace of the 4SPRR-SPR parallel robot is analyzed using the boundary extraction algorithm. Finally, the 3D model of the 4SPRR-SPR parallel robot is simulated using the ADAMS software to verify the reliability of the proposed algorithms. The simulation results demonstrate the effectiveness of the methods proposed in this study. In addition, the robot kinematics and workspace analysis methods described herein can be extended to other serial and parallel robots. This research provides a theoretical framework for trajectory planning of mechanisms, workspace optimization of robots, and robotic control.
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- 2020
27. Important gene-gene interaction of TNF-α and VDR on osteoporosis in community-dwelling elders
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Chiu-Shong Liu, Chuan Wei Yang, Chih Hsueh Lin, Li Na Liao, Fang Yang Wu, Chia Ing Li, Tsai-Chung Li, Cheng-Chieh Lin, and Wen-Yuan Lin
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0301 basic medicine ,Heredity ,Bone density ,Physiology ,Osteoporosis ,Organic chemistry ,Pathology and Laboratory Medicine ,Calcitriol receptor ,0302 clinical medicine ,Elderly ,Polymorphism (computer science) ,Bone Density ,Immune Physiology ,Genotype ,Medicine and Health Sciences ,Public and Occupational Health ,Vitamin D ,Connective Tissue Diseases ,Immune Response ,Aged, 80 and over ,Innate Immune System ,Multidisciplinary ,Vitamins ,Physical sciences ,Chemistry ,Genetic Mapping ,medicine.anatomical_structure ,Connective Tissue ,Area Under Curve ,Medicine ,Cytokines ,Female ,Independent Living ,Anatomy ,Research Article ,medicine.medical_specialty ,Science ,Immunology ,Variant Genotypes ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,Chemical compounds ,Signs and Symptoms ,Gene interaction ,Rheumatology ,Diagnostic Medicine ,Internal medicine ,Organic compounds ,medicine ,Genetics ,Humans ,Bone ,Femoral neck ,Aged ,Inflammation ,business.industry ,Tumor Necrosis Factor-alpha ,Genetic Variation ,Biology and Life Sciences ,Epistasis, Genetic ,Odds ratio ,Physical Activity ,Molecular Development ,medicine.disease ,030104 developmental biology ,Endocrinology ,Biological Tissue ,ROC Curve ,Age Groups ,Immune System ,People and Places ,Receptors, Calcitriol ,Population Groupings ,business ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Gene effects on osteoporosis have been studied separately and may have been masked by gene-gene and gene-environment interactions. We evaluated gene-gene and gene-physical activity interactions of the variants of tumor necrosis factor-α (TNF-α) and vitamin D receptor (VDR) genes on osteoporosis. A total of 472 elders were included. Seven variants (TNF-α: rs1799964, rs1800629, rs3093662; VDR: rs7975232, rs1544410, rs2239185, rs3782905) were genotyped. Bone mineral densities of the lumbar spine, femoral neck, and total hip were measured by dual-energy X-ray absorptiometry. Predictive models' ability to discriminate osteoporosis status was evaluated by areas under the receiver operating characteristics (AUROC) curve. After multivariable adjustment, significant interactions of TNF-α rs1800629 and VDR rs3782905 were observed on overall and lumbar spine osteoporosis. In elderly women, we found that those carrying the CG/CC genotype of VDR rs3782905 were significantly associated with increased odds of overall osteoporosis compared with those carrying the GG genotype of VDR rs3782905 among those carrying TNF-α rs1800629 GG genotype. The adjusted odds ratios (ORs) for VDR rs3782905 CG/CC genotype in elderly women carrying TNF-α rs1800629 AG/AA and GG genotypes were 0.1 (0.01, 0.98) and 3.54 (1.51, 8.30), respectively. We observed significant differences in AUROCs between the model with traditional covariates plus variants and their interaction term and the model with traditional covariates only (AUROCs: 0.77 and 0.81; p = 0.028). Although the sample size of this study may have been relatively small, our results suggest that the interaction of the CG/CC genotype of VDR rs3782905 with TNF-α rs1800629 GG genotype was associated with increased odds of overall and lumbar spine osteoporosis in elderly women.
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- 2019
28. Evaluation of single nucleotide polymorphisms in 6 candidate genes and carotid intima-media thickness in community-dwelling residents
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Chih Hsueh Lin, Chia Ing Li, Wen-Yuan Lin, Li Na Liao, Fang Yang Wu, Cheng-Chieh Lin, Chiu-Shong Liu, Chuan Wei Yang, and Tsai-Chung Li
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Male ,0301 basic medicine ,Candidate gene ,Heredity ,Physiology ,Glycobiology ,Cardiovascular Medicine ,030204 cardiovascular system & hematology ,Biochemistry ,Vascular Medicine ,Carotid Intima-Media Thickness ,0302 clinical medicine ,Genotype ,Medicine and Health Sciences ,Multidisciplinary ,biology ,Confounding ,Middle Aged ,Receptor, Endothelin A ,PON1 ,Body Fluids ,Genetic Mapping ,Blood ,C-Reactive Protein ,Physiological Parameters ,Cardiovascular Diseases ,cardiovascular system ,Medicine ,Female ,Independent Living ,Anatomy ,Research Article ,medicine.medical_specialty ,Science ,Variant Genotypes ,Single-nucleotide polymorphism ,Polymorphism, Single Nucleotide ,Blood Plasma ,Molecular Genetics ,03 medical and health sciences ,Internal medicine ,Genetics ,medicine ,Humans ,SNP ,Obesity ,Molecular Biology ,Alleles ,Glycoproteins ,Aryldialkylphosphatase ,business.industry ,Body Weight ,Paraoxonase ,Biology and Life Sciences ,Fibrinogen ,Atherosclerosis ,030104 developmental biology ,Endocrinology ,Intima-media thickness ,Genetic Loci ,biology.protein ,business - Abstract
Evidence suggests the existence of association between a large panel of modifiable biomarkers representing inflammation, coagulation, paraoxonase, and endothelial activation pathways and carotid atherosclerosis. Thus, this study investigated whether CRP, FGA, FGB, FGG, PON1, and EDNRA gene variants affected plasma hs-CRP, fibrinogen levels, and thickness of carotid intima media thickness (IMT). Nineteen single-nucleotide polymorphisms of CRP, FGA, FGB, FGG, PON1, and EDNRA genes were examined in 480 participants from 160 families. Carotid IMT was measured by ultrasound. Generalized linear models with generalized estimating equation were utilized to consider the dependence of subjects within families. In the recessive model, homozygotes for the minor alleles of rs1800789, rs1800790 and rs4220 SNPs in FGB gene indicated a reduced risk of IMT (Exp. β = 0.89, 0.89, 0.88), which remained significant after adjustment for confounding factors. Significant interaction effects between CRP SNP rs1130864 and rs3093059 and gender for IMT were observed with a significant association in men only. Men carrying minor-minor genotype of CRP SNP rs1130864 and rs3093059 had 0.70- and 0.78-fold lower IMT than men carrying minor-major/major-major genotype. We also observed that the interaction of CRP SNP rs1130864 and rs3093059 with obesity on IMT, hs-CRP and fibrinogen levels. These results support the hypothesis that inflammatory genes are involved in atherosclerosis, most likely via complex gene-gender and gene-obesity interactions.
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- 2020
29. Effect of continuous positive airway pressure on carotid intima-media thickness in patients with obstructive sleep apnea: A meta-analysis
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Zhi-Ming Cai, Li Lin, Yuming Ye, Qiao-Zhen Xu, Xue-Jun Lin, Zhi Wu, Yang-Wu Ou, Ya-Ping Huang, and Li-Da Chen
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Male ,Pulmonology ,Apnea ,medicine.medical_treatment ,lcsh:Medicine ,Cochrane Library ,Pathology and Laboratory Medicine ,Vascular Medicine ,Carotid Intima-Media Thickness ,0302 clinical medicine ,Mathematical and Statistical Techniques ,Medicine and Health Sciences ,Continuous positive airway pressure ,lcsh:Science ,Cognitive Impairment ,Stenosis ,Clinical Trials as Topic ,Sleep Apnea, Obstructive ,Multidisciplinary ,Continuous Positive Airway Pressure ,Cognitive Neurology ,Sleep apnea ,Middle Aged ,Carotid Arteries ,Neurology ,Research Design ,Meta-analysis ,Physical Sciences ,Cardiology ,cardiovascular system ,Observational Studies ,Female ,Statistics (Mathematics) ,Algorithms ,Research Article ,Adult ,medicine.medical_specialty ,Drug Research and Development ,Sleep Apnea ,Cognitive Neuroscience ,Research and Analysis Methods ,Sensitivity and Specificity ,03 medical and health sciences ,Signs and Symptoms ,Diagnostic Medicine ,Internal medicine ,medicine ,Humans ,In patient ,Clinical Trials ,cardiovascular diseases ,Statistical Methods ,Pharmacology ,business.industry ,lcsh:R ,Biology and Life Sciences ,medicine.disease ,Atherosclerosis ,Confidence interval ,Randomized Controlled Trials ,nervous system diseases ,respiratory tract diseases ,Obstructive sleep apnea ,030228 respiratory system ,Intima-media thickness ,Cognitive Science ,lcsh:Q ,Clinical Medicine ,business ,Sleep Disorders ,030217 neurology & neurosurgery ,Mathematics ,Meta-Analysis ,Neuroscience - Abstract
Objective Obstructive sleep apnea (OSA) is associated with increased carotid intima-media thickness (IMT), an early marker of atherosclerosis. Continuous positive airway pressure (CPAP) is the first-line treatment for OSA. A meta-analysis was performed to determine whether CPAP therapy could decrease carotid IMT. Methods The PubMed, Embase, Web of Science, and Cochrane library were searched before March, 2017. Weighted mean difference (WMD) was calculated to estimate the treatment effects of pre and post-CPAP therapy. Seven studies were examined and the meta-analysis was performed using STATA 12.0. Results There was no change of carotid IMT before and after CPAP treatment in OSA patients (WMD = 0.052, 95% confidence interval (CI) = -0.002 to 0.105, z = 1.90, p = 0.057). Meanwhile, meta-analysis of the two RCTs showed that carotid IMT was not changed in CPAP group when compared with control group (WMD = 0.002 95% CI = -0.125 to 0.129, z = 0.03, p = 0.976). Subgroup analyses indicated that carotid IMT was significantly decreased after CPAP use in more severe OSA patients (AHI≥50) (WMD = 0.073, 95% CI = 0.022 to 0.124, z = 2.80, p = 0.005) and patients with therapeutic duration ≥6 months (WMD = 0.121, 95% CI = 0.019 to 0.223, z = 2.32, p = 0.021). Conclusions CPAP had no impact on carotid IMT in OSA patients. However, carotid IMT was significantly decreased after CPAP treatment in more severe OSA patients and patients with longer CPAP usage.
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- 2017
30. Prognostic implication of EGFR gene mutations and histological classification in patients with resected stage I lung adenocarcinoma
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Meng-Heng Hsieh, Ying-Jen Chen, Chih-Wei Wang, Yueh-Fu Fang, Chun-Yu Lin, Yen-Mu Wu, and Ching-Yang Wu
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0301 basic medicine ,Oncology ,Male ,Lung Neoplasms ,Cancer Treatment ,lcsh:Medicine ,Gene mutation ,Lung and Intrathoracic Tumors ,0302 clinical medicine ,Adenocarcinomas ,Medicine and Health Sciences ,Epidermal growth factor receptor ,lcsh:Science ,Multidisciplinary ,biology ,Adenocarcinoma of the Lung ,Middle Aged ,Prognosis ,ErbB Receptors ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Adenocarcinoma ,Female ,Anatomy ,Research Article ,medicine.medical_specialty ,Histology ,Surgical and Invasive Medical Procedures ,Carcinomas ,03 medical and health sciences ,Internal medicine ,medicine ,Adenocarcinoma of the lung ,Genetics ,Humans ,Point Mutation ,Lung cancer ,Survival rate ,Aged ,Lung ,business.industry ,lcsh:R ,Cancers and Neoplasms ,Biology and Life Sciences ,medicine.disease ,030104 developmental biology ,Mutation ,biology.protein ,lcsh:Q ,business - Abstract
Introduction The prognostic value of epidermal growth factor receptor (EGFR) mutations and the correlation between EGFR mutations and the new International Association for the Study of Lung Cancer/American Thoracic Society/European Respiratory Society (IASLC/ATS/ERS) histological classification remain controversial. The current study aimed to investigate the pure prognostic role of EGFR mutations in treatment-naive patients with resected stage I lung adenocarcinoma. Methods We retrospectively reviewed 373 patients with stage I pulmonary non-small-cell lung cancer who underwent complete surgical resection between January 2010 and May 2014. The tumors were classified according to IASLC/ATS/ERS criteria. EGFR mutation status was determined by established methods. Results A total of 120 patients were included for analysis; 87 had tumors with EGFR mutations and 33 had wild-type tumors. More low- and intermediate-grade tumors had EGFR mutations, and nearly half of the high-grade tumors were wild-type (75.7% versus 46.2%, p = 0.041). Patients with low-grade tumors had significantly greater median disease-free survival (DFS) (76.8 versus 13 months, p < 0.0001) and better overall survival (OS) (median OS not reached, p = 0.0003) than those with intermediate- and high-grade tumors. Tumor recurrence was 41.4% and 30.3% in mutant and wild-type patients. The 5-years survival rate was 54% and 71.2%. Multivariate analysis revealed that the new histological classification and the pathologic stage were independent predictors of both DFS and OS. EGFR mutation status had no prognostic implications. Conclusion Low grade tumors according to IASLC/ATS/ERS histological classification and the pathologic stage IA tumors of resected stage I lung adenocarcinomas independently predict better DFS and OS. EGFR mutations were frequently seen in histologically low- and intermediate-grade tumors but not a prognostic factor.
- Published
- 2017
31. 5-aminolevulinic acid-mediated photodynamic therapy and its strain-dependent combined effect with antibiotics on Staphylococcus aureus biofilm
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Li-Min Guo, Chun-Hong Liu, Chunquan Zheng, Ke-Qing Zhao, Qing-Zhao Zhang, Yang Wu, Di Qu, Xian-Hui Li, and Chen Yang
- Subjects
0301 basic medicine ,Confocal Microscopy ,Staphylococcus ,medicine.medical_treatment ,Antibiotics ,lcsh:Medicine ,Photodynamic therapy ,Pathology and Laboratory Medicine ,Toxicology ,medicine.disease_cause ,Medicine and Health Sciences ,Photosensitizer ,lcsh:Science ,Microscopy ,Multidisciplinary ,biology ,Antimicrobials ,Chemistry ,Drugs ,Light Microscopy ,Staphylococcal Infections ,Anti-Bacterial Agents ,Bacterial Pathogens ,Medical Microbiology ,Staphylococcus aureus ,Methicillin-resistant Staphylococcus aureus ,Pathogens ,Research Article ,030103 biophysics ,medicine.drug_class ,Microbial Sensitivity Tests ,Research and Analysis Methods ,Microbiology ,03 medical and health sciences ,Antibiotic resistance ,Vancomycin ,Microbial Control ,medicine ,Humans ,Microbial Pathogens ,Pharmacology ,Bacteria ,Toxicity ,lcsh:R ,Organisms ,Biofilm ,Biology and Life Sciences ,Bacteriology ,Aminolevulinic Acid ,biology.organism_classification ,Multiple drug resistance ,030104 developmental biology ,Photochemotherapy ,Biofilms ,Antibiotic Resistance ,lcsh:Q ,Antimicrobial Resistance ,Bacterial Biofilms ,Confocal Laser Microscopy - Abstract
Staphylococcus aureus (S. aureus) is hard to be eradicated, not only due to the emergence of antibiotic resistant strains but also because of its ability to form biofilm. Antibiotics are the major approach to treating biofilm infections, but their effects are unsatisfactory. One of the potential alternative treatments for controlling biofilm infections is photodynamic therapy (PDT), which requires the administration of photosensitizer, followed by light activation. 5-aminolevulinic acid (ALA), a natural photosensitizer prodrug, presents favorable characteristics, such as easy penetration and rapid clearance. These advantages enable ALA-based PDT (ALA-PDT) to be well-tolerated by patients and it can be repeatedly applied without cumulative toxicity or serious side effects. ALA-PDT has been proven to be an effective treatment for multidrug resistant pathogens; however, the study of its effect on S. aureus biofilm is limited. Here, we established our PDT system based on the utilization of ALA and a light-emitting diode, and we tested the effect of ALA-PDT on S. aureus biofilm as well as the combined effect of ALA-PDT and antibiotics on S. aureus biofilm. Our results showed that ALA-PDT has a strong antibacterial effect on S. aureus biofilm, which was confirmed by the confocal laser scanning microscope. We also found that lethal photosensitization occurred predominantly in the upper layer of the biofilm, while the residual live bacteria were located in the lower layer of the biofilm. In addition, the improved bactericidal effect was observed in the combined treatment group but in a strain-dependent manner. Our results suggest that ALA-PDT is a potential alternative approach for future clinical use to treat S. aureus biofilm-associated infections, and some patients may benefit from the combined treatment of ALA-PDT and antibiotics, but drug sensitivity testing should be performed in advance.
- Published
- 2017
32. Myocyte enhancer factor 2A promotes proliferation and its inhibition attenuates myogenic differentiation via myozenin 2 in bovine skeletal muscle myoblast
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Wang, Ya-Ning, primary, Yang, Wu-Cai, additional, Li, Pei-Wei, additional, Wang, Hong-Bao, additional, Zhang, Ying-Ying, additional, and Zan, Lin-Sen, additional
- Published
- 2018
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33. Angiotensin receptor blockers are associated with lower mortality than ACE inhibitors in predialytic stage 5 chronic kidney disease: A nationwide study of therapy with renin-angiotensin system blockade
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Lin, Chih-Ching, primary, Wu, Yu-Te, additional, Yang, Wu-Chang, additional, Tsai, Min-Juei, additional, Liu, Jia-Sin, additional, Yang, Chi-Yu, additional, Li, Szu-Yuan, additional, Ou, Shuo-Ming, additional, Tarng, Der-Cherng, additional, and Hsu, Chih-Cheng, additional
- Published
- 2017
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34. Metabolic phenotyping in the mouse model of urinary tract infection shows that 3-hydroxybutyrate in plasma is associated with infection
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Han, Pei, primary, Huang, Yong, additional, Xie, Yumin, additional, Yang, Wu, additional, Wang, Yaoyao, additional, Xiang, Wenying, additional, Hylands, Peter J., additional, and Legido-Quigley, Cristina, additional
- Published
- 2017
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35. Effect of continuous positive airway pressure on carotid intima-media thickness in patients with obstructive sleep apnea: A meta-analysis
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Chen, Li-Da, primary, Lin, Li, additional, Lin, Xue-Jun, additional, Ou, Yang-Wu, additional, Wu, Zhi, additional, Ye, Yu-Ming, additional, Xu, Qiao-Zhen, additional, Huang, Ya-Ping, additional, and Cai, Zhi-Ming, additional
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- 2017
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36. The Impact of Variable Degrees of Freedom and Scale Parameters in Bayesian Methods for Genomic Prediction in Chinese Simmental Beef Cattle
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Xue Gao, Yanhui Wang, Huijiang Gao, Yang Wu, Lingyang Xu, Bo Zhu, Hong Niu, Jianfeng Liu, Junya Li, Lupei Zhang, Miao Zhu, Jicai Jiang, and Yan Chen
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0301 basic medicine ,Male ,Normal Distribution ,lcsh:Medicine ,Beef cattle ,Bayes' theorem ,Mathematical and Statistical Techniques ,Animal Products ,Statistics ,Medicine and Health Sciences ,lcsh:Science ,Mathematics ,Hyperparameter ,Mammals ,Multidisciplinary ,Small number ,Simulation and Modeling ,Agriculture ,04 agricultural and veterinary sciences ,Genomics ,Ruminants ,Vertebrates ,Physical Sciences ,Female ,Beef ,Research Article ,Veterinary Medicine ,Livestock ,Meat ,Bayesian probability ,Quantitative Trait Loci ,Bayesian Method ,Research and Analysis Methods ,Genome Complexity ,Polymorphism, Single Nucleotide ,Normal distribution ,03 medical and health sciences ,Bovines ,Prior probability ,Genetics ,Animals ,Nutrition ,Models, Statistical ,business.industry ,lcsh:R ,0402 animal and dairy science ,Organisms ,Biology and Life Sciences ,Computational Biology ,Bayes Theorem ,Heritability ,Probability Theory ,Probability Distribution ,040201 dairy & animal science ,Biotechnology ,Diet ,030104 developmental biology ,Genetic Loci ,Food ,Amniotes ,Cattle ,Veterinary Science ,Livestock Care ,lcsh:Q ,business - Abstract
Three conventional Bayesian approaches (BayesA, BayesB and BayesCπ) have been demonstrated to be powerful in predicting genomic merit for complex traits in livestock. A priori, these Bayesian models assume that the non-zero SNP effects (marginally) follow a t-distribution depending on two fixed hyperparameters, degrees of freedom and scale parameters. In this study, we performed genomic prediction in Chinese Simmental beef cattle and treated degrees of freedom and scale parameters as unknown with inappropriate priors. Furthermore, we compared the modified methods (BayesFA, BayesFB and BayesFCπ) with their corresponding counterparts using simulation datasets. We found that the modified methods with distribution assumed to the two hyperparameters were beneficial for improving the predictive accuracy. Our results showed that the predictive accuracies of the modified methods were slightly higher than those of their counterparts especially for traits with low heritability and a small number of QTLs. Moreover, cross-validation analysis for three traits, namely carcass weight, live weight and tenderloin weight, in 1136 Simmental beef cattle suggested that predictive accuracy of BayesFCπ noticeably outperformed BayesCπ with the highest increase (3.8%) for live weight using the cohort masking cross-validation.
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- 2016
37. Performance Evaluation Model for Application Layer Firewalls
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Xuan, Shichang, primary, Yang, Wu, additional, Dong, Hui, additional, and Zhang, Jiangchuan, additional
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- 2016
- Full Text
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38. Outcomes and Treatment of Lumbosacral Spinal Tuberculosis: A Retrospective Study of 53 Patients
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Yang Wu, Jinming Zhao, Tenglong Jiang, Kun Wang, Mitra Fowdur, and Maolin He
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Adult ,Male ,medicine.medical_specialty ,Sacrum ,Adolescent ,Decompression ,medicine.medical_treatment ,Antitubercular Agents ,lcsh:Medicine ,Lumbar vertebrae ,Blood Sedimentation ,Young Adult ,medicine ,Back pain ,Animals ,Humans ,lcsh:Science ,Child ,Aged ,Retrospective Studies ,Multidisciplinary ,Lumbar Vertebrae ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Retrospective cohort study ,Middle Aged ,Decompression, Surgical ,Surgery ,Radiography ,medicine.anatomical_structure ,C-Reactive Protein ,Spinal Fusion ,Treatment Outcome ,Debridement ,Back Pain ,Spinal fusion ,Erythrocyte sedimentation rate ,Lumbosacral angle ,lcsh:Q ,Female ,Tuberculosis, Spinal ,medicine.symptom ,business ,Lumbosacral joint ,Research Article ,Follow-Up Studies - Abstract
Study Strategy A retrospective clinic study. Purpose To evaluate the efficacy of conservative and surgical treatment for lumbosacral tuberculosis. Methods This study retrospectively reviewed 53 patients with lumbosacral tuberculosis who were treated in our institution between January 2005 and January 2011. There were 29 males and 24 females with average ages of 37.53 ± 17.28 years (range 6–72 years). 11 patients were given only anti-TB drugs; the remainder underwent anterior debridement, interbody fusion with and without instrumentation, or one-stage anterior debridement combined with posterior instrumentation. Outcome data for these patients included neurologic status, lumbosacral angle, erythrocyte sedimentation rate value(ESR) and C-reactive protein value(CRP) were assessed before and after treatment. Results The mean lumbosacral angles were 23.00°± 2.90°in the conservatively treated patients and 22.36°± 3.92o in the surgically treated patients. At the final follow-up, this had improved to 24.10o ± 2.96°in the conservatively treated patients and 28.13° ± 1.93°in the surgically treated patients (all P < 0.05). There were statistically significant differences before and after treatment in terms of ESR and CRP (all P < 0.05). All patients achieved bone fusion. The mean follow-up period was 32.34 ± 8.13 months (range 18 to 55 months). The neurological deficit did not worsen in any of the patients. Conclusions It has been proven that conservative and surgical treatments are safe and effective and produce good clinical outcomes for patients with lumbosacral tuberculosis. The advantages of operation include thoroughness of debridement, decompression of the spinal cord, and adequate spinal stabilization.
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- 2015
39. Basic Study for Ultrasound-Based Navigation for Pedicle Screw Insertion Using Transmission and Backscattered Methods
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Chengcheng Liu, Chao Li, Xiaodong Zhu, Dean Ta, Yingchuan Zhao, Chuanfeng Wang, Yang Wu, Mingyuan Yang, Ming Li, Xianzhao Wei, Ziqiang Chen, Yushu Bai, Luo Bei'er, Kailiang Xu, Xiao Chen, Kai Chen, Bing Wu, and Xiao Zhai
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Materials science ,medicine.medical_treatment ,lcsh:Medicine ,Cadaver ,Pedicle Screws ,medicine ,Humans ,lcsh:Science ,Multidisciplinary ,business.industry ,Attenuation ,lcsh:R ,Ultrasound ,Navigation system ,Anatomy ,Acoustics ,medicine.anatomical_structure ,Spinal Fusion ,Ultrasonic Waves ,Spinal fusion ,lcsh:Q ,Ultrasonic sensor ,Cortical bone ,business ,Cancellous bone ,Biomedical engineering ,Research Article - Abstract
The purpose of this study was to understand the acoustic properties of human vertebral cancellous bone and to study the feasibility of ultrasound-based navigation for posterior pedicle screw fixation in spinal fusion surgery. Fourteen human vertebral specimens were disarticulated from seven un-embalmed cadavers (four males, three females, 73.14 ± 9.87 years, two specimens from each cadaver). Seven specimens were used to measure the transmission, including tests of attenuation and phase velocity, while the other seven specimens were used for backscattered measurements to inspect the depth of penetration and A-Mode signals. Five pairs of unfocused broadband ultrasonic transducers were used for the detection, with center frequencies of 0.5 MHz, 1 MHz, 1.5 MHz, 2.25 MHz, and 3.5 MHz. As a result, good and stable results were documented. With increased frequency, the attenuation increased (P0.05). At about 0.6 cm away from the cortical bone, warning signals were easily observed from the backscattered measurements. In conclusion, the ultrasonic system proved to be an effective, moveable, and real-time imaging navigation system. However, how ultrasonic navigation will benefit pedicle screw insertion in spinal surgery needs to be determined. Therefore, ultrasound-guided pedicle screw implantation is theoretically effective and promising.
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- 2015
40. Genome-Wide Association Studies Using Haplotypes and Individual SNPs in Simmental Cattle
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Huizhong Fan, Junya Li, Yanhui Wang, Yan Chen, Yang Wu, Xue Gao, Lupei Zhang, Huijiang Gao, and Hongyan Ren
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Male ,Meat ,Science ,Genome-wide association study ,Biology ,Quantitative trait locus ,Research and Analysis Methods ,Polymorphism, Single Nucleotide ,Genetics ,Animals ,Genetic association ,Multidisciplinary ,Haplotype ,Biology and Life Sciences ,Agriculture ,Genomics ,Tag SNP ,SNP genotyping ,Bovine genome ,Haplotypes ,Animal Studies ,Medicine ,Cattle ,Haplotype estimation ,Research Article ,Genome-Wide Association Study - Abstract
Recent advances in high-throughput genotyping technologies have provided the opportunity to map genes using associations between complex traits and markers. Genome-wide association studies (GWAS) based on either a single marker or haplotype have identified genetic variants and underlying genetic mechanisms of quantitative traits. Prompted by the achievements of studies examining economic traits in cattle and to verify the consistency of these two methods using real data, the current study was conducted to construct the haplotype structure in the bovine genome and to detect relevant genes genuinely affecting a carcass trait and a meat quality trait. Using the Illumina BovineHD BeadChip, 942 young bulls with genotyping data were introduced as a reference population to identify the genes in the beef cattle genome significantly associated with foreshank weight and triglyceride levels. In total, 92,553 haplotype blocks were detected in the genome. The regions of high linkage disequilibrium extended up to approximately 200 kb, and the size of haplotype blocks ranged from 22 bp to 199,266 bp. Additionally, the individual SNP analysis and the haplotype-based analysis detected similar regions and common SNPs for these two representative traits. A total of 12 and 7 SNPs in the bovine genome were significantly associated with foreshank weight and triglyceride levels, respectively. By comparison, 4 and 5 haplotype blocks containing the majority of significant SNPs were strongly associated with foreshank weight and triglyceride levels, respectively. In addition, 36 SNPs with high linkage disequilibrium were detected in the GNAQ gene, a potential hotspot that may play a crucial role for regulating carcass trait components.
- Published
- 2014
41. Dual Effects of Alpha-Arbutin on Monophenolase and Diphenolase Activities of Mushroom Tyrosinase
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Yang Wu, Peng Zhang, Liang Qin, Yi-Ming Chen, and Youting Liu
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Tyrosinase ,lcsh:Medicine ,Bioengineering ,Biochemistry ,Protein Chemistry ,Melanin ,chemistry.chemical_compound ,Chemical Biology ,Biomechanics ,lcsh:Science ,chemistry.chemical_classification ,Multidisciplinary ,biology ,Chemistry ,Activator (genetics) ,Plant Biochemistry ,Monophenol Monooxygenase ,Arbutin ,lcsh:R ,Substrate (chemistry) ,Active site ,Biology and Life Sciences ,Biochemical Reactivation ,Biochemical Activity ,Enzyme structure ,Enzyme ,biology.protein ,Biocatalysis ,Enzymology ,lcsh:Q ,Agaricales ,Oxidoreductases ,Research Article ,Biotechnology - Abstract
The effects of α-arbutin on the monophenolase and diphenolase activities of mushroom tyrosinase were investigated. The results showed that α-arbutin inhibited monophenolase activity but it activated diphenolase activity. For monophenolase activity, IC50 value was 4.5 mmol · L(-1) and 4.18 mmol · L(-1) of α-arbutin could extend the lag time from 40.5 s to 167.3 s. Alpha- arbutin is proposed to be regarded as a triphenolic substrate by the enzyme during catalyzation, leading to the suicide inactivation of the active site of tyrosinase. For diphenolase activity, α-arbutin acted as an activator and its activation mechanism was mixed type activation. To reveal such activation, it should be mainly refered to the conformational changes in tyrosinase caused by the interaction of α-arbutin with residues located at the entrance to the active site, and the decrease of the effect of suicide inactivation.
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- 2014
42. Removing Phosphorus from Aqueous Solutions Using Lanthanum Modified Pine Needles
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Wang, Xianze, primary, Liu, Zhongmou, additional, Liu, Jiancong, additional, Huo, Mingxin, additional, Huo, Hongliang, additional, and Yang, Wu, additional
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- 2015
- Full Text
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43. Removing of Disinfection By-Product Precursors from Surface Water by Using Magnetic Graphene Oxide
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Liu, Zhongmou, primary, Wang, Xianze, additional, Luo, Zhen, additional, Huo, Mingxin, additional, Wu, Jinghui, additional, Huo, Hongliang, additional, and Yang, Wu, additional
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- 2015
- Full Text
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44. Diagnostic Accuracy of Urine Protein/Creatinine Ratio Is Influenced by Urine Concentration
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Yang, Chih-Yu, primary, Chen, Fu-An, additional, Chen, Chun-Fan, additional, Liu, Wen-Sheng, additional, Shih, Chia-Jen, additional, Ou, Shuo-Ming, additional, Yang, Wu-Chang, additional, Lin, Chih-Ching, additional, and Yang, An-Hang, additional
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- 2015
- Full Text
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45. Identification of a Group of GABAergic Neurons in the Dorsomedial Area of the Locus Coeruleus
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Shuang Zhang, Yang Wu, Weiwei Zhong, Max F. Oginsky, Christopher M. Johnson, Ningren Cui, Xin Jin, Brian Bondy, Chun Jiang, and Shanshan Li
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Male ,0301 basic medicine ,Patch-Clamp Techniques ,Light ,Physiology ,lcsh:Medicine ,Action Potentials ,Channelrhodopsin ,Cell Separation ,Biochemistry ,Synaptic Transmission ,Inhibitions ,Mice ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,GABAergic Neurons ,lcsh:Science ,Neurons ,Light Pulses ,Brain Mapping ,Multidisciplinary ,Neuronal Morphology ,Physics ,Electromagnetic Radiation ,Anatomy ,Electrophysiology ,Bioassays and Physiological Analysis ,medicine.anatomical_structure ,Cell Tracking ,Physical Sciences ,GABAergic ,Locus Coeruleus ,Cellular Types ,Research Article ,Yellow Fluorescent Protein ,Neurophysiology ,Mice, Transgenic ,Neurotransmission ,Optogenetics ,Biology ,Research and Analysis Methods ,Inhibitory postsynaptic potential ,Membrane Potential ,03 medical and health sciences ,Interneurons ,medicine ,Animals ,Behavior ,lcsh:R ,Biology and Life Sciences ,Proteins ,Cell Biology ,Neurophysiological Analysis ,Electrophysiological Phenomena ,Mice, Inbred C57BL ,Luminescent Proteins ,030104 developmental biology ,nervous system ,Cellular Neuroscience ,Locus coeruleus ,lcsh:Q ,Nucleus ,Neuroscience ,030217 neurology & neurosurgery - Abstract
The locus coeruleus (LC)-norepinephrine (NE) system in the brainstem plays a critical role in a variety of behaviors is an important target of pharmacological intervention to several neurological disorders. Although GABA is the major inhibitory neurotransmitter of LC neurons, the modulation of LC neuronal firing activity by local GABAergic interneurons remains poorly understood with respect to their precise location, intrinsic membrane properties and synaptic modulation. Here, we took an optogenetic approach to address these questions. Channelrhodopsin (ChR2) in a tandem with the yellow fluorescent protein (YFP) was expressed in GABAergic neurons under the control of glutamic acid decarboxylase 2 (GAD2) promoter. Immediately dorsomedial to the LC nucleus, a group of GABAergic neurons was observed. They had small soma and were densely packed in a small area, which we named the dorsomedial LC or dmLC nucleus. These GABAergic neurons showed fast firing activity, strong inward rectification and spike frequency adaptation. Lateral inhibition among these GABAergic neurons was observed. Optostimulation of the dmLC area drastically inhibited LC neuronal firing frequency, expanded the spike intervals, and reset their pacemaking activity. Analysis of the light evoked inhibitory postsynaptic currents (IPSCs) indicated that they were monosynaptic. Such light evoked IPSCs were not seen in slices where this group of GABAergic neurons was absent. Thus, an isolated group of GABAergic neurons is demonstrated in the LC area, whose location, somatic morphology and intrinsic membrane properties are clearly distinguishable from adjacent LC neurons. They interact with each and may inhibit LC neurons as well as a part of local neuronal circuitry in the LC.
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- 2016
46. Rice osa-miR171c Mediates Phase Change from Vegetative to Reproductive Development and Shoot Apical Meristem Maintenance by Repressing Four OsHAM Transcription Factors
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Fan, Tian, primary, Li, Xiumei, additional, Yang, Wu, additional, Xia, Kuaifei, additional, Ouyang, Jie, additional, and Zhang, Mingyong, additional
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- 2015
- Full Text
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47. Basic Study for Ultrasound-Based Navigation for Pedicle Screw Insertion Using Transmission and Backscattered Methods
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Chen, Ziqiang, primary, Wu, Bing, additional, Zhai, Xiao, additional, Bai, Yushu, additional, Zhu, Xiaodong, additional, Luo, Beier, additional, Chen, Xiao, additional, Li, Chao, additional, Yang, Mingyuan, additional, Xu, Kailiang, additional, Liu, Chengcheng, additional, Wang, Chuanfeng, additional, Zhao, Yingchuan, additional, Wei, Xianzhao, additional, Chen, Kai, additional, Yang, Wu, additional, Ta, Dean, additional, and Li, Ming, additional
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- 2015
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48. Impact of Water Content and Temperature on the Degradation of Cry1Ac Protein in Leaves and Buds of Bt Cotton in the Soil
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Zhang, Mei-jun, primary, Feng, Mei-chen, additional, Xiao, Lu-jie, additional, Song, Xiao-yan, additional, Yang, Wu-de, additional, and Ding, Guang-wei, additional
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- 2015
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49. Novel Detection of Insecticide Resistance Related P450 Genes and Transcriptome Analysis of the Hemimetabolous Pest Erthesina fullo (Thunberg) (Hemiptera: Heteroptera)
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Yang Liu, Hao-Yang Wu, Wenjun Bu, and Qiang Xie
- Subjects
Genetics ,Genome evolution ,Multidisciplinary ,biology ,Sequence analysis ,Gene Expression Profiling ,Science ,Genome, Insect ,High-Throughput Nucleotide Sequencing ,Molecular Sequence Annotation ,biology.organism_classification ,Hemiptera ,18S ribosomal RNA ,Heteroptera ,Insecticide Resistance ,Gene expression profiling ,Transcriptome ,Cytochrome P-450 Enzyme System ,GenBank ,Animals ,Medicine ,Gene ,Research Article - Abstract
Erthesina fullo (Thunberg, 1783) is an economically important heteropteran species in China. Since only three nucleotide sequences of this species (COI, 16S rRNA, and 18S rRNA) appear in the GenBank database so far, no analysis of the molecular mechanisms underlying E. fullo's resistance to insecticide and environmental stress has been accomplished. We reported a de novo assembled and annotated transcriptome for adult E. fullo using the Illumina sequence system. A total of 53,359,458 clean reads of 4.8 billion nucleotides (nt) were assembled into 27,488 unigenes with an average length of 750 bp, of which 17,743 (64.55%) were annotated. In the present study, we identified 88 putative cytochrome P450 sequences and analyzed the evolution of cytochrome P450 superfamilies, genes of the CYP3 clan related to metabolizing xenobiotics and plant natural compounds, in E. fullo, increasing the candidate genes for the molecular mechanisms of insecticide resistance in P450. The sequenced transcriptome greatly expands the available genomic information and could allow a better understanding of the mechanisms of insecticide resistance at the systems biology level.
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- 2015
50. Polydatin Restores Endothelium-Dependent Relaxation in Rat Aorta Rings Impaired by High Glucose: A Novel Insight into the PPARβ-NO Signaling Pathway
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Changqing Liu, Qingsong Jiang, Bo Huang, Cuiping Tang, Hongmei Qiu, Lai Xue, Weimin Du, and Yang Wu
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Male ,medicine.medical_specialty ,Nitric Oxide Synthase Type III ,Endothelium ,Vasodilator Agents ,lcsh:Medicine ,Nitric Oxide Synthase Type II ,Aorta, Thoracic ,Vasodilation ,Pharmacology ,Nitric Oxide ,Nitric oxide ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Enzyme activator ,Glucosides ,Enos ,Fallopia japonica ,medicine.artery ,Internal medicine ,Stilbenes ,medicine ,Animals ,Thoracic aorta ,lcsh:Science ,PPAR-beta ,Multidisciplinary ,biology ,business.industry ,lcsh:R ,Endothelial Cells ,biology.organism_classification ,Rats ,Enzyme Activation ,Glucose ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Hyperglycemia ,lcsh:Q ,Female ,Plant Preparations ,Signal transduction ,business ,Signal Transduction ,Research Article - Abstract
Polydatin, a natural component from Polygonum Cuspidatum, has important therapeutic effects on metabolic syndrome. A novel therapeutic strategy using polydatin to improve vascular function has recently been proposed to treat diabetes-related cardiovascular complications. However, the biological role and molecular basis of polydatin's action on vascular endothelial cells (VECs)-mediated vasodilatation under diabetes-related hyperglycemia condition remain elusive. The present study aimed to assess the contribution of polydatin in restoring endothelium-dependent relaxation and to determine the details of its underlying mechanism. By measuring endothelium-dependent relaxation, we found that acetylcholine-induced vasodilation was impaired by elevated glucose (55 mmol/L); however, polydatin (1, 3, 10 μmol/L) could restore the relaxation in a dose-dependent manner. Polydatin could also improve the histological damage to endothelial cells in the thoracic aorta. Polydatin's effects were mediated via promoting the expression of endothelial NO synthase (eNOS), enhancing eNOS activity and decreasing the inducible NOS (iNOS) level, finally resulting in a beneficial increase in NO release, which probably, at least in part, through activation of the PPARβ signaling pathway. The results provided a novel insight into polydatin action, via PPARβ-NO signaling pathways, in restoring endothelial function in high glucose conditions. The results also indicated the potential utility of polydatin to treat diabetes related cardiovascular diseases.
- Published
- 2015
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