1. Role of nitric oxide and signaling pathways modulating the stimulatory effect of snake venom secretory PLA 2 S on non-opsonized zymosan phagocytosis by macrophages.
- Author
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Zuliani JP, Gutiérrez JM, and Teixeira C
- Subjects
- Animals, Mice, Phospholipases A2, Secretory metabolism, Phagocytosis drug effects, Zymosan pharmacology, Signal Transduction drug effects, Nitric Oxide metabolism, Bothrops, Crotalid Venoms, Macrophages drug effects
- Abstract
The phagocytic activity of macrophages activated with MT-II, a Lys-49 PLA
2 homolog, and MT-III, an Asp-49 PLA2 , from Bothrops asper snake venom, was investigated in this study using a pharmacological approach. Stimulating thioglycollate-elicited macrophages with both venom components enhanced their ability to phagocytose non-opsonized zymosan particles. MT-II and MT-III-induced phagocytosis was drastically inhibited by pretreating cells with L-NAME, aminoguanidine or L-NIL, cNOS or iNOS inhibitors, or with ODQ (sGC inhibitor) or Rp-cGMPS (PKG inhibitor). These results indicate that the NO/sGC/GMP/PKG pathway plays an essential role in the β-glucan-mediated phagocytosis induced in macrophages by these venom-secretory PLA2 s., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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